LOCUS       NZ_CP022413            13503 bp    DNA     linear   CON 29-JAN-2025
DEFINITION  Blautia hansenii DSM 20583 chromosome, complete genome.
ACCESSION   NZ_CP022413 REGION: 515673..529175
VERSION     NZ_CP022413.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07340700
            Assembly: GCF_002222595.2
KEYWORDS    RefSeq.
SOURCE      Blautia hansenii DSM 20583
  ORGANISM  Blautia hansenii DSM 20583
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Wang,J.R. and Hansen,J.
  TITLE     Economical hybrid assembly and finishing of multiplexed bacterial
            genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13503)
  AUTHORS   Wang,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2017) Genetics, University of North Carolina at
            Chapel Hill, 120 Mason Farm Road 5000 D, Genetic Medicine Building
            Cb#7264, Chapel Hill, NC 27599, USA
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 13503)
  AUTHORS   Wang,J.R. and Hansen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2017) Genetics, University of North Carolina at
            Chapel Hill, 120 Mason Farm Road 5000 D, Genetic Medicine Building
            Cb#7264, Chapel Hill, NC 27599, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP022413.2.
            
            On Sep 29, 2017 this sequence version replaced NZ_CP022413.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 17-AUG-2017
            Assembly Method        :: SPAdes v. 3.10.0
            Assembly Name          :: b_hansenii_nanopore_peillumina
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 40x
            Sequencing Technology  :: Oxford Nanopore MinION, Illumina HiSeq
                                      4000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002222595.2-RS_2025_01_26
            Annotation Date                   :: 01/26/2025 11:45:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 2,985
            Genes (coding)                    :: 2,936
            CDSs (with protein)               :: 2,936
            Genes (RNA)                       :: 90
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 9 of 50
            Pseudo Genes (incomplete)         :: 38 of 50
            Pseudo Genes (internal stop)      :: 11 of 50
            Pseudo Genes (multiple problems)  :: 8 of 50
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13503
                     /organism="Blautia hansenii DSM 20583"
                     /mol_type="genomic DNA"
                     /strain="DSM 20583"
                     /culture_collection="DSM:20583"
                     /type_material="type strain of Streptococcus hansenii"
                     /db_xref="taxon:537007"
                     /collection_date="2016-08-01"
     gene            complement(1..981)
                     /locus_tag="CGC63_RS02540"
                     /old_locus_tag="CGC63_02520"
     CDS             complement(1..981)
                     /locus_tag="CGC63_RS02540"
                     /old_locus_tag="CGC63_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221158.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_004221158.1"
                     /translation="MSKVKITDIAERSGVSMTTVSRYFNHPELISQTTQDKIKTAIKE
                     LNYQQDNLARILVTGESNLVGVIFPHLHLSFYTELLNQLIKYGKEKGYNLIVYTSNDS
                     REEELQLIQNLISYRIKGLILLSHLLSTQDIENLPVPVISIERVGGNFKQINNDNFTG
                     GKLAANLLIRNNCEVFIHINNDFREDWPSFKRILGFEYEVKDLCYERFIENDLTDPYL
                     PKATEAMNKLVQHITTKYTGKKIGVFCSNDDIASLFERQCLKNNIDIPNTIELIGYDN
                     SPASNYAVHPITSIDQNISLMAQIAIQSLENYIPYESMVPASLIEKETTS"
     gene            1339..2556
                     /locus_tag="CGC63_RS02545"
                     /old_locus_tag="CGC63_02525"
     CDS             1339..2556
                     /locus_tag="CGC63_RS02545"
                     /old_locus_tag="CGC63_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221156.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_004221156.1"
                     /translation="MKKKILSIVLAVAMAGTMLAGCGAKKEAKNKNSITVLVESGSPA
                     EALAKETAASFEEETGCEVIIDAVAYTGMYDKLSTEIKAKQVAHDVACMDVVWLAAFA
                     DAIEAINDADTSDFLPTLQESGTIDGSLLGYPMWVNTKVLIYRKDLIPEDKVPQTWEE
                     YQALAKELKTDDMSGTTVFGSGSDAVCSFLDFACQAGAEGLVFDKDGNVNITDQAYVD
                     ALNFMVENATADYTPDDSLATAATESQELFTNGKTAMQLNWSHQYPAAVEALGADKVG
                     CAPMIGGSAGVGATTGPWYECVMKGSENKEMAKKYVEYMYNHNADYMDLTLKIAGRTS
                     VYEEAGKETGNEHTTAVLETLRAEQSQARPMVTTWAQIEEVLIGVVEASLGGADVNET
                     LESAKAEIEAIAR"
     gene            2651..3535
                     /locus_tag="CGC63_RS02550"
                     /old_locus_tag="CGC63_02530"
     CDS             2651..3535
                     /locus_tag="CGC63_RS02550"
                     /old_locus_tag="CGC63_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221152.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_004221152.1"
                     /translation="MRLISRKTLLLFFLPGAIFMGAFLIYPIITMVVDSFFEIGITGD
                     KTFVGLENYIRAFTAGGFLKQLKNTLLYIVIAVSIETAIGLLFALFFELNYKGSKIIR
                     SLMMAPLMIAPLVAGLTWKLMMSSSFGIVNELLAKIGILSSSSDILWLADEKWSLLAC
                     CIADIWLTTPFMMLMILAGLQGLDSSMLEAAKMDGASLLQQVFAIKLPVIKPVLLTAL
                     SVRIIDAARTFDIIWAMTEGGPNSSSETLSIIIYKTLTRYNDVGYASAMAVVFIAVLV
                     IFTLVFMQSLWNPKKKNC"
     gene            3560..4390
                     /locus_tag="CGC63_RS02555"
                     /old_locus_tag="CGC63_02535"
     CDS             3560..4390
                     /locus_tag="CGC63_RS02555"
                     /old_locus_tag="CGC63_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341277.1"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_004221150.1"
                     /translation="MNRKGEIGKKLGIGLSVIFTVFWLVPYIYVICCSFKPGSEVIAV
                     PPSFFPKAFSIENFTGLLERMDALQFLWNSLSVSLISTVIALILGSLAAYAIQRSGAK
                     LSIILVVLVLCLKMIPTSSIVVPIYELICNLGLYDTKIALIIVYAAINMPFVMWTMLS
                     FYEGIPTTLDEAAFVDGASSFKTFWKVILPICKPGLATAFIFTLFLAWNDFLVALLLT
                     STNAKTFTVGLAGFLSAYNLDLGPMCAGAFLFSFPVMIISLAAQKYIVQGMIAGAVKG
                     "
     gene            4409..5893
                     /locus_tag="CGC63_RS02560"
                     /old_locus_tag="CGC63_02540"
     CDS             4409..5893
                     /locus_tag="CGC63_RS02560"
                     /old_locus_tag="CGC63_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221148.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_004221148.1"
                     /translation="MSKELLKQNIEKAQFEIDRKKDMIKSGKMRQHYHFMPQTGWLND
                     PNGLIYFRGKYHFFFQHNPYSGFWDSMHWGHAVSDDMLHWEYLPLALVPSEKYDNHQK
                     GGCFSGSAIEHDGKLFLMYTGVTNEGNGYEQVQCIAYSEDGIHFEKYEGNPVLTAPEG
                     IPTDMFRDPKVWKHEDTYYMVCGASRNNRGMALLYRSKNMLNWEFFNVLAESRGEWGF
                     MWECPDFYPIGDKYVLTFSPMGSGDHTSVYLVGDFDYETGSFYSHVSGEINWGFDYYA
                     PQSFLAPDGRRIMVGWANEWEWMPSWKDWGPTYKEGWCGSFNIPMEVRLMKDSTLQFL
                     PIKEIESLRQGGQFWDNLVVTEDELELKAGDGVTFELKMKIDLEKTDADKLEFTLRCG
                     EGKKTVCMFDFKKAEMTVNRNNADGWSKGISRSVMYLNNKKDLDVHILSDQSSLEIFT
                     SEYQNNHSNNIFAGNFQNQLKVRAYGGSVAIKDIETYGLKDCYC"
     gene            5906..6880
                     /locus_tag="CGC63_RS02565"
                     /old_locus_tag="CGC63_02545"
     CDS             5906..6880
                     /locus_tag="CGC63_RS02565"
                     /old_locus_tag="CGC63_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="WP_004221146.1"
                     /translation="MMQKEIDVTALGELLIDFTENGVSSQGNALLEANPGGAPCNVLA
                     MLGKLGKKTAFIGKVGNDQFGNMLRNTIESVGIDAEGLVIDDKVHTTLAFVHTYPDGD
                     RDFSFYRNPGADMMLRKEEVSLDKICASKIFHFGTLSFTHAGIRAASQYAIQCAKEAG
                     ALISFDPNLREPLWENLEDARKAIEYGMECCDILKISDNELTFMTGEKDYDKGAVLLQ
                     QKYQIPLVCVTLGKDGSRAYYKGLTIKAEPFLQKNTIETTGAGDTFTGCMLNTVLDKG
                     LDNLTGEDIRGMLRFANAGAALITTKRGALRVMPEKEEIEALLEEQTE"
     gene            6901..8256
                     /locus_tag="CGC63_RS02570"
                     /old_locus_tag="CGC63_02550"
     CDS             6901..8256
                     /locus_tag="CGC63_RS02570"
                     /old_locus_tag="CGC63_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="WP_004221144.1"
                     /translation="MSSKNYYDVTKWNVGNPYEDIGEVINSIIADIKKRQTDSNMNEG
                     GKPGAVIYIPSGDYHLRTQVVIDISYLKIMGSGHGFVSSSIRYNLPENEWADLHEVWP
                     GGSRILVDLSPKPGDEESAGAAFYVERDGNPRISSVEFENFCIDGLHFVDDGSGMPNP
                     ENTYTNGKTGIYIGSAQDSFRLTGMGIVYLEHGVTIYHADALSIHDNFIAECGNCIEL
                     RGWGQASKITDNLMGAGYKGYSIFAENFGGLLITANNIFPRGTSSVHFRNVARSTITA
                     NRFHSFYPGMLVFEGNCSENMVSSNHFLRDNEPWAPMLEYDNGLDDLYGILCISGNNN
                     SVIANHISEIIHTQSIKPAGAKPVIIHIVSGKGNYISDNHIVATTEATDEQTKASDSC
                     FSAQVDALTTTDKLVALDVTAVLVEKESVQNTILDSGSEGQVIIDRTVNAFRATPVPG
                     V"
     gene            complement(8360..10225)
                     /locus_tag="CGC63_RS02575"
                     /old_locus_tag="CGC63_02555"
     CDS             complement(8360..10225)
                     /locus_tag="CGC63_RS02575"
                     /old_locus_tag="CGC63_02555"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221141.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_242648432.1"
                     /translation="MTGKKTTIQSTWKMNIRAWKIWWKLCPRFFFSAGISSMVKALTP
                     YVTIFLSAQIINELSGNQDVAVLAKWVILTLTYTSLCALLNSILKHWLNYEKSSAQSI
                     DRQVYMKKMLDLDYEDIERQPIFDLYSQIIQTKYWSGWGIYKTLLFFEELVTAFFQIL
                     GGISLSVSLFAAKVPKGSELEPINHPLFAVGILMLLLLIAVLSPICKTMGDKYMTNYA
                     KEARLGNRFFYFFGFMSHDKKRAADLRMYQQQENICHPRMAECTMFSAHSDIAKSAMG
                     PMGLLHALSQGMSVILTGVVYLYVCLKAWAGAFGVGSVTQYIGAITQLFGGVSAFLTT
                     VGAMQSNGEFLVSCFHFLDIPNRMYQGSLTTEKRSDRQYEVEFRDVSFRYPDTEQWAL
                     RHINLKFQIGKRLAVVGMNGSGKTTFIKLLCRLYDPTEGQIFLNEIDIRKYKYDDYIN
                     IFSVVFQDFQLLSLPLGENVAGAARYDSELAKSCLKNAGFETRFQSMPKGLDTYLYKD
                     LDENGIQISGGEAQKIAIARALYKDAPFIILDEPTAALDPVAEAEIYSKFNDIVTDKT
                     TVYISHRLSSCKFCDEIAVFHEGNVVQKGTHEELISDKTGKYYELWNAQAQYYNE"
     gene            complement(10215..12038)
                     /locus_tag="CGC63_RS02580"
                     /old_locus_tag="CGC63_02560"
     CDS             complement(10215..12038)
                     /locus_tag="CGC63_RS02580"
                     /old_locus_tag="CGC63_02560"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221139.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_004221139.1"
                     /translation="MEEQQKPKYNMWQNTGFMVKNAWKNCKSVLFLCVALAVTTATKS
                     AAELFLAPTILEKVETTAPLSELLFTIAVFSCGLLFLSGLKQYIDTNTQFGRISVRTS
                     ILVQISDKIAKTSYLNLMDAKFNDMESKASNACSSNVEATEYVWTIWTSILTNVLGFS
                     IYLALLSHLNPFLVCIAIFTTLLGYLISKRTSRWGYEHREEEGEYAKQLDYLHDVATK
                     RSYAKDIRIFGLDTWINDVWTSILRLYQGFLRKRESVYIWADIADLLLTLLRNGAAYA
                     YLLWLTLTQGLNASEFLLYFTAISGFTQWIGGILEMFAQLHKCSLDLSTVREFLEYPE
                     PFLFEEGKPVSKDDFSAYELCLKNVSFRYPKAKQDTIHCLNLTIHAEEKLAVVGLNGA
                     GKTTLVKLICGFLDPTEGQILLNGKDIRQYNRQNYYSLFSAVFQDFSVLEASVAENVA
                     QHTTHIDEKKVWNCLKQAGLFEKISSLPKNINTIIGRQVYDNGIELSGGQIQRLMLAR
                     ALYKNAPVLILDEPTAALDPIAENDIYLKYNEMTAHKTSVFISHRLASTRFCHRILFL
                     EKGRIAEQGTHEELIALQGKYAKLFDIQSQYYKEGGIQRDR"
     gene            complement(12134..12796)
                     /locus_tag="CGC63_RS02585"
                     /old_locus_tag="CGC63_02565"
     CDS             complement(12134..12796)
                     /locus_tag="CGC63_RS02585"
                     /old_locus_tag="CGC63_02565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221137.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0000156 - phosphorelay response regulator activity
                     [Evidence IEA]; GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]; GO:0004673 - protein histidine
                     kinase activity [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="WP_004221137.1"
                     /translation="MKLFSKRMDKELAIYQQELIETHYQEVDNMYRQIRGWRHDYRNH
                     IQTMKAYAATENWEALSHYLDLLDEDLTTVDTVIKTGNPMTDAILNSKISLAKSKNIQ
                     VIADAHIPVKLTSSEIDLCCIIGNLFDNAIDASLKLPEEHRLIRIYMGMKNTQLYISF
                     TNFTAEKKMKKEGNLFRSTKGAGHGFGLARIDAIVERQEGYISRNSEDGAFTTEILLP
                     QL"
     gene            complement(12805..13503)
                     /locus_tag="CGC63_RS02590"
                     /old_locus_tag="CGC63_02570"
     CDS             complement(12805..13503)
                     /locus_tag="CGC63_RS02590"
                     /old_locus_tag="CGC63_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221135.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000156 - phosphorelay response regulator activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="WP_004221135.1"
                     /translation="MYYQIAICDDSKIDTQYITNFVKCWAQEANISVHIDAFPSAEAF
                     LFHYEEKKDYDILLLDVEMGAMNGVTLAKTLRKENDTIQIVFLTGYSDYISEGYEVAA
                     LHYLLKPVQKEKLFSVLYRALDKVKKNEKILNLETGSEILRIPIYEIRYADVTGNYVT
                     IHAKKDYTLKMTLGKLEKLLDERFYRAGRSVIVNITNISRVTKAEIKLRDGTVIALPR
                     GAYEGINRAIINMR"
ORIGIN      
        1 ttaactggtt gtttcttttt caatcaatga agctggtacc atgctttcgt atggtatata
       61 gttttccagt gactgaattg caatctgcgc cataagggaa atattctggt cgatagaggt
      121 aatgggatga acggcatagt tggatgcagg tgagttgtcg tatccaatca attcaatggt
      181 gttgggaata tcaatattat ttttgaggca ctgacgttca aaaagacttg caatatcgtc
      241 atttgagcag aaaactccta tttttttacc ggtgtatttt gtggtaatat gctgaaccag
      301 tttattcatg gcttctgtag ctttgggcag gtatggatct gtgagatcat tttcaataaa
      361 ccgttcataa caaagatctt ttacctcata ttcaaatccc aggattcgtt taaaggatgg
      421 ccagtcttct cgaaaatcat tattaatatg aatgaaaact tcacagttgt tacgtatcaa
      481 aagatttgcg gcaagtttgc caccagtaaa gttatcattg ttaatttgct taaaattacc
      541 gccgacacgt tcaatagaga taaccgggac tggcaggttt tcaatgtcct gagtggaaag
      601 aagatgactc aaaagaatca gtcctttgat tcggtaggaa attagatttt gaataagctg
      661 taattcttct tctctggaat cgttggaagt atagacgatt agattatagc ctttttcttt
      721 accgtatttt attagttgat tgagtaattc tgtatagaag cttaaatgta ggtgggggaa
      781 aataacccca accagattgg attctccggt taccagaatg cgggccagat tgtcctgctg
      841 gtaattcaat tctttaattg ctgttttgat tttatcctgt gttgtctgcg atatgagctc
      901 aggatggttg aagtaacggg aaacggtagt catggaaaca ccgctccgtt cggcaatatc
      961 tgtaattttg actttactca taaaagaacc tcacttattt ggtaaatata gtcttgtgtt
     1021 atatacgata tgagacaaaa tatatctcac tttatgaatt tgattatatc acaagattat
     1081 gaaaaatgga agaataaatg aaaataattt catgaaaaaa ttttcataca agttttattg
     1141 atgaaaagaa ataccgtaaa aataaagaaa taagctatga aatatataca aattaaacaa
     1201 aaaaatattg tgaatagtgc tgaatagtga aaaaatacat gaaaacgatt gacattataa
     1261 atttcatgac atacaatcga atcatcagct agaaagtaac gaagagaaat taaaaaagaa
     1321 aacaggagga agataggaat gaagaaaaaa attttaagta ttgtgcttgc agtagcaatg
     1381 gcaggaacaa tgttagcggg gtgtggagct aaaaaagaag caaaaaataa aaacagtatt
     1441 acagtactgg tggaaagtgg ttcgccggca gaggcacttg caaaagaaac agcagcatct
     1501 tttgaagaag aaactggatg tgaagttatt attgatgcag ttgcttatac aggaatgtat
     1561 gacaaacttt ctacagagat taaagcaaag caggttgcac atgatgtggc atgtatggat
     1621 gtggtatggt tagcagcatt tgcagatgca attgaagcaa tcaatgatgc ggatacaagt
     1681 gatttcctgc caaccctaca ggaaagtgga acaattgatg gtagcctttt aggatatcct
     1741 atgtgggtaa atacaaaagt gttgatttac agaaaagatc ttattccgga agataaagtt
     1801 ccgcagacat gggaagagta tcaggcactt gcaaaagaac tgaaaacaga tgatatgagt
     1861 ggaacaacag tattcggaag cggttctgat gcagtatgtt cattccttga ttttgcatgt
     1921 caggctggtg cagaagggct tgtatttgat aaagatggaa atgtaaacat tacagatcag
     1981 gcttatgtag acgcattgaa ttttatggta gagaacgcaa ctgctgatta tactccagac
     2041 gattcacttg ctacagcagc cacagagtct caggaactct ttacaaacgg aaaaacagca
     2101 atgcagctga actggagtca tcagtatcca gcagcagtag aagctcttgg agcagataaa
     2161 gttggatgtg caccgatgat tggcggaagt gcaggtgttg gtgcaacaac aggaccttgg
     2221 tatgagtgtg taatgaaagg ttctgaaaac aaagagatgg ctaaaaaata tgtagaatat
     2281 atgtataatc acaatgcgga ttatatggat cttacattaa agattgcagg aagaacatct
     2341 gtatatgaag aggccggaaa agaaactggt aatgaacata caacagcagt tctcgaaaca
     2401 ttaagagcag aacagtcaca ggccagacct atggtaacta catgggctca gattgaagaa
     2461 gttttaattg gtgtcgtaga agccagtctg ggtggcgctg atgttaatga aacattggaa
     2521 agtgcaaagg ctgaaattga ggcaattgca agatagctga attaacgcaa ataaatggat
     2581 tagcagagga tattctaaaa agataatcaa gaaagaatat cctttttgct tagaaaaggt
     2641 ggtaaataga atgagattga tttcaagaaa aaccttatta ttattctttc tgccaggtgc
     2701 catatttatg ggggcatttc ttatatatcc gattattaca atggtagttg acagtttctt
     2761 tgagattgga attacagggg ataagacatt tgttggactg gaaaattaca ttcgtgcttt
     2821 tacagcgggt ggatttttaa aacagttaaa gaatacatta ctttatattg taatagcggt
     2881 gagtattgaa actgcaatcg gtttattgtt tgcattgttc tttgaattga attataaagg
     2941 aagcaaaata atccgttcat taatgatggc acctcttatg atagcaccat tggtagctgg
     3001 tcttacatgg aaattgatga tgagttccag ctttggaatt gttaatgaat tactggcaaa
     3061 aatcggaatt ttatcaagca gcagtgatat tctttggctt gcagatgaaa agtggtcatt
     3121 gcttgcatgt tgcattgcag atatctggct cacaacaccg tttatgatgt tgatgatatt
     3181 ggctggattg cagggactgg attccagtat gcttgaagct gccaaaatgg atggagcaag
     3241 tttactgcag caggtgtttg ctattaagct tccggttatc aaaccggttt tgttgaccgc
     3301 attgtctgta agaattattg atgccgccag aacttttgat attatctggg cgatgactga
     3361 gggaggtccg aatagttctt ctgaaacact cagcatcatt atctacaaga cattgacacg
     3421 atataatgat gttggttatg caagtgcgat ggcagtggta tttattgcag tattagtaat
     3481 atttacgctt gtgtttatgc agagtttatg gaatccgaag aaaaagaatt gttagtgggt
     3541 ttgataggag gcgaaaagag tgaacagaaa aggtgaaatc ggaaaaaagc tgggaatcgg
     3601 tttatcagtg atctttacag tgttctggct tgttccgtac atatatgtaa tttgttgttc
     3661 gtttaaaccg ggttcagagg taattgcagt tcctccaagt ttttttccaa aagcattctc
     3721 tattgagaac tttacgggac tgcttgagag aatggatgca cttcaatttt tgtggaacag
     3781 tttgagtgtt tcgttgataa gtacagtgat tgcattaatt ttgggttcgc ttgcagcgta
     3841 tgcgattcaa cgttcaggcg ctaaattatc aattatattg gttgttttgg ttttgtgttt
     3901 gaagatgata cctacttcaa gtattgtagt tccgatttac gaactgattt gtaatttggg
     3961 tctgtatgat acaaaaattg ctttgattat tgtttatgca gctattaata tgccgtttgt
     4021 aatgtggaca atgttaagct tctatgaagg tattccgaca acattggatg aggcggcatt
     4081 tgtggatggt gcaagtagct ttaaaacatt ttggaaagtt attctgccaa tttgtaaacc
     4141 ggggttggca acagcattta tatttacatt gttccttgca tggaatgatt tccttgtagc
     4201 attattactg accagtacaa atgcaaagac atttacagtt ggtctggctg gatttttatc
     4261 agcatacaat ctggatttgg gaccgatgtg tgcaggcgca tttttgttta gttttccggt
     4321 aatgattatt tctcttgcag ctcaaaaata tattgttcag ggaatgatag ctggagctgt
     4381 aaaaggttaa aaataatgga ggagtcctat gtctaaagaa ttattgaaac agaatattga
     4441 aaaggctcag tttgagatag acagaaaaaa agatatgata aaaagcggca aaatgcgtca
     4501 gcattaccat tttatgccgc agacaggatg gttgaatgat cccaatggac tgatttattt
     4561 cagaggaaag taccatttct ttttccagca taatccctat agtggatttt gggacagtat
     4621 gcattggggg catgcagtca gtgatgacat gcttcattgg gagtatttac cgttagcact
     4681 ggttcctagt gaaaaatatg ataatcatca aaaaggtggc tgcttttcag gaagtgctat
     4741 tgaacatgat ggaaaactgt ttctgatgta cactggtgta acaaatgaag gcaatgggta
     4801 tgaacaggtg cagtgtattg catatagtga agatggaatt cattttgaaa aatatgaggg
     4861 aaatccggtg ctgactgcac cggaaggaat cccgacagat atgttccgtg atcccaaagt
     4921 atggaagcat gaagatactt attatatggt atgtggcgca agtcgcaata acagagggat
     4981 ggcactgctg tatcgttcca agaatatgct gaactgggag ttctttaatg tgctggcgga
     5041 aagccgtgga gaatggggat ttatgtggga atgcccagat ttttatccaa ttggtgataa
     5101 atatgttctg acattttcgc ctatgggttc aggtgaccat acttcggtgt atttggttgg
     5161 cgattttgat tatgaaacgg gcagttttta ttcccatgtc agtggagaga ttaactgggg
     5221 atttgattat tatgcaccgc agtcattctt ggcgccggat ggaagaagaa ttatggtagg
     5281 atgggccaat gaatgggaat ggatgccatc gtggaaagat tggggaccaa catacaagga
     5341 aggttggtgt ggatctttta atattccaat ggaagtacgt ttaatgaaag attccacctt
     5401 acagtttctt ccaattaagg aaatagaatc tttgagacag ggtggacagt tttgggacaa
     5461 tcttgtggtt acagaagatg aactggaact gaaggccgga gatggtgtga catttgaatt
     5521 gaagatgaag attgacctgg agaaaacaga tgcagataag ctggaattta ccttgcgttg
     5581 tggagaaggt aagaagacag tgtgtatgtt cgattttaaa aaggcagaaa tgactgtaaa
     5641 tcgaaataat gcagatgggt ggagcaaagg catttcaaga agcgttatgt atctgaataa
     5701 taaaaaagac ttggatgtgc atattttatc tgatcagagt tcccttgaaa tttttacgag
     5761 tgaatatcag aataatcatt ccaataatat atttgcagga aattttcaga atcagttgaa
     5821 agtccgggct tatggaggaa gtgtagcaat caaagatatt gaaacatacg gattgaaaga
     5881 ttgctattgt taggaggaaa attagatgat gcaaaaggaa atagatgtga cagctttggg
     5941 cgaacttctg attgatttta cagagaatgg cgtcagcagt cagggaaatg cattattaga
     6001 agcaaatcca ggtggggctc catgtaatgt gctggcaatg cttggaaagc tgggcaaaaa
     6061 aacagcattt atcggaaagg ttggaaacga tcaatttggt aatatgctaa gaaatacgat
     6121 agagtctgtt ggaattgatg cagaaggttt agtgattgat gacaaggtac ataccacatt
     6181 ggcatttgta catacttatc cagatggtga cagagatttt tctttttacc gtaatccggg
     6241 agcggacatg atgttgagaa aagaagaggt ttctttggat aagatatgtg catccaaaat
     6301 ctttcatttt ggaacgttat cctttactca tgcgggaatc agggcagctt cacaatatgc
     6361 aattcagtgt gcgaaagaag ctggagcgtt gatttccttt gatcctaatt tacgtgaacc
     6421 attgtgggag aatcttgagg acgcacgaaa agctattgag tatggtatgg agtgttgtga
     6481 tatcttaaaa atatcggaca atgaactgac ttttatgaca ggtgaaaagg attatgacaa
     6541 aggagcagtt cttcttcagc agaaatatca gattccactt gtatgtgtta ctctgggaaa
     6601 agatggaagt cgggcatact ataaggggct tacgattaaa gcggagccat ttttgcagaa
     6661 aaacacgata gaaacaacgg gtgcaggaga tacctttacc ggatgtatgc tgaatacagt
     6721 tttggataag gggcttgata atcttaccgg agaggacatc agagggatgc ttcgatttgc
     6781 aaatgcaggc gcagctctga ttacaacaaa gcgaggtgct ttgagggtta tgccggaaaa
     6841 agaagaaatt gaagcgttat tagaagagca gacagagtaa acatatagga ggatttattg
     6901 atgtcaagta aaaattatta tgacgttaca aaatggaacg ttggaaatcc ttacgaggat
     6961 attggggaag taatcaacag tattattgcg gatattaaaa agcgacaaac agattcaaat
     7021 atgaatgaag gtggaaaacc gggagcagtc atttacattc cgtctggtga ttatcatctt
     7081 cgtacacaag tagtgattga tatcagttac ttgaaaatta tgggttcagg acatggtttt
     7141 gtatcatcca gtattcgtta taatcttccg gagaatgaat gggcagattt acatgaagtg
     7201 tggccgggcg gaagccgtat ccttgttgac ctttctccaa aaccaggaga tgaagaatcg
     7261 gcaggagcag cattctatgt tgagcgagat ggaaatcccc gaattagttc tgtagaattt
     7321 gaaaacttct gtattgatgg tttgcatttt gtggatgatg gctcgggaat gccaaatcca
     7381 gaaaatacat atactaatgg aaaaacaggt atttatattg gaagtgcaca ggattcattc
     7441 aggcttacag gtatgggaat cgtatattta gaacatgggg ttacgattta tcatgcagat
     7501 gcgttgtcta ttcatgataa tttcatagca gaatgcggta attgcataga gctgagagga
     7561 tggggacagg catctaaaat aacagataat ctgatgggcg caggttataa gggatattcc
     7621 atctttgcgg aaaattttgg tggacttttg attacagcaa ataatatttt ccctcgaggc
     7681 acaagcagtg ttcatttccg taatgtggca cgttccacta ttacagccaa ccgatttcat
     7741 tccttctatc cgggaatgct ggtatttgaa ggaaactgtt cggaaaatat ggtttcttca
     7801 aatcattttt tacgtgacaa tgaaccatgg gcgcctatgt tggaatatga taatggatta
     7861 gatgatttgt acggtattct gtgtatcagt ggtaataata attcggtaat tgccaaccat
     7921 atttcggaaa taatccatac acagtctatc aagccggccg gggcaaaacc tgtaatcata
     7981 catattgttt caggtaaagg aaattatatt tcggataacc atattgttgc aactaccgaa
     8041 gctacggatg agcagacaaa ggcatcagat tcttgctttt ctgcacaagt agatgcactg
     8101 acgactactg ataagttggt tgcgcttgat gtaacagctg ttttggtaga aaaagaatcc
     8161 gttcaaaata cgattcttga ctccggaagc gagggacagg ttattataga caggactgta
     8221 aatgctttta gagccactcc agtgccagga gtataaaatt atggtgcaaa tagcaagtat
     8281 atgaaagaaa aagagccttg gcgtgatgtc agggctcttt tagtggaacg ataaaaagta
     8341 ttccaaatgc tcggtatttt tattcattat aatactgcgc ctgtgcattc cagagttcat
     8401 aatattttcc agttttatcg ctaatcaatt cctcatgtgt tcctttctga actacatttc
     8461 cctcatgaaa tacggcaatt tcatcacaaa atttgcaaga agacagacgg tgggaaatat
     8521 aaacggttgt tttatccgtt acaatatcat tgaacttact gtaaatttct gcctctgcaa
     8581 caggatctaa agcagcagtc ggttcatcta aaataatgaa cggagcatct ttgtagagtg
     8641 ctcttgcaat ggcaattttc tgggcttctc ctccggatat ctgaataccg ttttcatcca
     8701 aatctttata aagataagtg tctaagcctt tgggcatgga ctgaaagcgt gtttcaaatc
     8761 cggcattttt taagcaggat tttgccagtt cagagtcata gcgtgcagcg ccggcaacat
     8821 tttctcccag aggcagcgac aatagctgaa aatcttgaaa aacaacggaa aaaatgttga
     8881 tataatcgtc atatttatac tttcgaatgt caatttcatt taagaaaatc tgtccttctg
     8941 tgggatcata caggcggcac aaaagtttga taaacgtagt ttttccactg ccgttcatac
     9001 ctactacggc aaggcgtttg cctatttgga actttaaatt aatgtgccgc aaagcccatt
     9061 gttctgtatc aggatagcga aaggaaacat ctcgaaattc cacttcatat tgtctgtccg
     9121 accgtttttc cgtggtaaga ctgccttgat acatgcggtt tggaatatcc agaaaatgaa
     9181 aacaggagac taaaaattca ccgttgcttt gcatagcgcc tactgtggta aggaaggcag
     9241 aaacaccgcc aaaaagctga gtaattgctc cgatatattg tgtgacagag cctactccga
     9301 aagcgcctgc ccatgctttt aaacatacat acaaatatac aacgccggtt aaaataacag
     9361 acataccttg tgaaagtgcg tgcaaaagtc ccataggtcc catggcagat tttgcgatat
     9421 cggaatgtgc gctgaacatg gtacattccg ccattcgagg atgacagata ttttcctgtt
     9481 gttgatacat acgaaggtct gccgctcttt ttttatcatg gctcataaaa ccaaagaaat
     9541 agaaaaagcg attgccaaga cgtgcctcct tggcataatt ggtcatatat ttgtctccca
     9601 tggttttgca tataggggaa agaactgcaa taagcagtaa aagcatgaga attccaacgg
     9661 caaaaagagg atgattgatg ggttccagtt cacttccttt agggactttg gctgcaaata
     9721 aactgacaga caggctaatt cccccaagga tttggaaaaa tgcagtaacc agttcttcga
     9781 aaaaaaggag ggttttataa attccccatc cggaccaata tttcgtctga ataatctgag
     9841 aatataaatc aaaaatcggc tgccgttcta tgtcctcata atctaaatcc agcatttttt
     9901 tcatgtaaac ctgtcggtca atggactgag cagagctttt ttcataattg agccaatgct
     9961 ttaaaatgct gtttaacaga gcacagagtg aggtataggt cagagtcaga attacccatt
    10021 ttgcaaggac ggccacatct tgatttccgg aaagctcatt gatgatttga gcggacagaa
    10081 agatggttac ataaggagtt aatgctttta ccatggagga aatgcctgcc gaaaaaaaga
    10141 agcggggaca gagcttccac cagattttcc aagctcttat attcattttc caagtagatt
    10201 gtatagttgt ttttttaccg gtcacgttga attcctccct ccttataata ttggctttgt
    10261 atatcaaaaa gtttggcata tttgccctgt aatgcaatta attcttcatg agttccctgc
    10321 tctgcaatgc ggcctttttc cagaaacaga atgcggtgac aaaagcgggt agaggcaagg
    10381 cggtgagaaa taaaaacaga tgttttatgg gcggtcattt cattatattt gagataaata
    10441 tcattctcgg caatcggatc aagagcagca gtaggctcgt ctaaaatgag aacaggagca
    10501 tttttataaa gcgctcttgc cagcattaaa cgctgtatct gccctccgga aagctcaatg
    10561 ccgttatcat aaacctgtct gccgataatg gtatttatat ttttaggcaa agaagaaatc
    10621 ttttcaaaaa gtcctgcctg tttcaaacaa ttccaaactt ttttctcatc aatatgtgtt
    10681 gtgtgctggg caacattttc agcaacactt gcctctaata cagaaaaatc ctgaaataca
    10741 gcagaaaata atgagtaata attttgccga ttgtattgtc gaatatcctt tccgtttaga
    10801 aggatttgtc cttccgttgg gtctaaaaat ccgcaaatca gtttcaccag agtagttttt
    10861 ccggcgccgt ttagacctac cactgcaagc ttttcttcgg catgtatcgt aagattgaga
    10921 cagtgaatag tgtcctgttt cgccttagga tagcggaaag atacgttttt caagcaaagt
    10981 tcgtaagcgg aaaaatcatc tttggaaaca ggttttcctt cttcaaaaag gaagggctcc
    11041 ggatattcta aaaattccct taccgtggat aaatcaaggc tgcatttgtg taattgcgca
    11101 aacatttcca aaattccgcc tatccactga gtaaagccgc ttattgctgt gaaataaagc
    11161 agaaattctg aagcatttag tccctgtgtc aaggtaagcc ataacaggta agcataagca
    11221 gctccgtttc gcagaagcgt caggagtaaa tcagcaatgt ctgcccagat atacacagac
    11281 tcacgtttgc gaagaaatcc ctgatatagc cttaaaatac tcgtccacac atcgtttatc
    11341 catgtatcca gaccaaagat acggatgtct tttgcataag aacgctttgt ggcaacatca
    11401 tgaagataat caagctgctt tgcgtattct ccttcttctt cccgatgttc atatccccat
    11461 ctgctcgttc ttttacttat cagatagccc agaagcgtag tgaaaattgc gatacagacc
    11521 aggaagggat taagatgaga caaaagcgcc agatagatgg aaaatcccag aacatttgta
    11581 aggatagaag tccagattgt ccagacatat tctgttgctt ctacgttact ggaacaggca
    11641 ttggaagcct tgctttccat atcgttaaac tttgcatcca ttagattgag ataggaagtt
    11701 ttggctattt tgtcggaaat ttgtaccaga atagatgtgc ggacggaaat acgcccaaac
    11761 tgtgtattgg tatcaatata ttgttttaat ccggatagaa aaagcaatcc acagctaaat
    11821 acggcaatgg taaaaagtaa ttctgaaagc ggggcagttg tctctacttt ttccagaatt
    11881 gtaggagcaa gaaaaagctc tgcggctgat tttgtagcag ttgtaactgc aagagcgaca
    11941 cataaaaaca aaacgctttt gcagtttttc caagcatttt tcaccataaa tccggtattt
    12001 tgccacatat tatattttgg tttttgttgt tcttccattt gtgtttcacc tccaaggtta
    12061 ctttagcata aaaattcgtc cgccatatac tttggggacg aattgttttg tgacggtgat
    12121 gaattgtaaa aaattatagc tgaggcagta aaatttctgt ggtaaaagct ccgtcttcac
    12181 tgtttctgct gatgtagccc tcctgacgtt caacaatggc gtcaattcgt gccagaccaa
    12241 agccatgtcc ggctcctttt gtactgcgaa agagattacc ctcttttttc atcttttttt
    12301 cggcagtaaa gtttgtaaaa gaaatataaa gctgcgtatt tttcataccc atatagatgc
    12361 gaatgagacg atgttcttca ggaagcttca gactggcatc aatggcattg tcaaaaagat
    12421 ttccgataat acagcataaa tcaatttctg aagatgttaa ttttacagga atatgggcgt
    12481 ctgcaattac ctgaatattt tttgactttg caagagaaat tttggaattc agaatggcat
    12541 ctgtcatagg atttccagtt ttaataacgg tatctaccgt agttaaatct tcatccaata
    12601 aatccagata atggctgagc gcttcccaat tttcagtggc agcataggct ttcatagttt
    12661 gaatatgatt gcgataatca tgccgccagc ctcgtatttg ccggtacata ttgtccactt
    12721 cctgatagtg agtttctata agttcttgct gatagattgc cagttcttta tccattcttt
    12781 ttgaaaaaag tttcatattt tgtcttacct catgttaata attgcccgat ttataccttc
    12841 ataagcgcct ctgggaagtg cgataacagt tccgtcacgg agcttaattt ctgcttttgt
    12901 tactcggctg atattggtta tatttacgat tacagaacgc cctgcacgat aaaaacgttc
    12961 gtcaagaagt ttttctaatt tacctaaagt catttttaat gtgtaatctt ttttggcatg
    13021 aatagttaca taatttcctg ttacatcggc ataacggatt tcataaatgg gaattcgcag
    13081 gatctcactt ccggtttcta aattcaggat tttttcattc ttttttacct tatcaagagc
    13141 acgataaagt acagaaaaca gcttttcttt ttgtaccggt tttaggagat aatgaagggc
    13201 ggcaacctca tagccttcgg aaatatagtc agaatagccc gtaagaaaaa caatctggat
    13261 ggtatcgttt tcttttctga gggttttggc taaggtgacg ccattcatag cgcccatttc
    13321 aacatctaaa agaagaatat cgtaatcctt cttttcttca taatgaaaca gaaatgcctc
    13381 tgcggaggga aatgcgtcaa tatgtacgga aatattcgct tcctgcgccc aacactttac
    13441 aaaatttgtg atgtattggg tatctatttt gctgtcatcg caaattgcta tttgataata
    13501 cat
//
