LOCUS       NZ_CP084061            13877 bp    DNA     linear   CON 14-DEC-2024
DEFINITION  Blautia parvula strain NBRC 113351 chromosome, complete genome.
ACCESSION   NZ_CP084061 REGION: 2698236..2712112
VERSION     NZ_CP084061.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21529767
            Assembly: GCF_020215665.1
KEYWORDS    RefSeq.
SOURCE      Blautia parvula
  ORGANISM  Blautia parvula
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 13877)
  AUTHORS   Shimamura,M., Yamazoe,A., Miura,T. and Kawasaki,H.
  TITLE     Blautia puncti sp. nov., isolated from Japanese fecal
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13877)
  AUTHORS   Miura,T., Shimamura,M., Furukawa,M., Ohyama,Y. and Kawasaki,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2021) Biotechnology center, Biological Resource
            Center, National Institute of Technology and Evaluation (NBRC),
            2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP084061.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Unicycler v. v0.4.7
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 200x
            Sequencing Technology  :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020215665.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 15:37:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,663
            CDSs (total)                      :: 5,579
            Genes (coding)                    :: 5,491
            CDSs (with protein)               :: 5,491
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 16 of 88
            Pseudo Genes (incomplete)         :: 69 of 88
            Pseudo Genes (internal stop)      :: 12 of 88
            Pseudo Genes (multiple problems)  :: 8 of 88
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13877
                     /organism="Blautia parvula"
                     /mol_type="genomic DNA"
                     /strain="NBRC 113351"
                     /isolation_source="faecal matter"
                     /host="Homo sapiens"
                     /type_material="type strain of Blautia parvula"
                     /db_xref="taxon:2877527"
                     /geo_loc_name="Japan"
                     /collection_date="2017-06-27"
     gene            complement(1..978)
                     /locus_tag="LC508_RS12670"
                     /old_locus_tag="LC508_12670"
     CDS             complement(1..978)
                     /locus_tag="LC508_RS12670"
                     /old_locus_tag="LC508_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267319.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_103731127.1"
                     /translation="MANIRDVARAAGVGIGTVSRALNDSGYVEAGKKEKIRRIAKELN
                     YRPSELSRSFVQKKSGLVGIVLPDVSFPFYGAFLKYAELELNHIGYKTLVCNTLGVQK
                     RVSDTLDLIEAGSLDGMIINTDVTREEIERMKGMPVVSFERVLAKEIPMVASDHIKGG
                     QIAAKLLKENGCKNVMILGARFATKVHAENRIKECRRILEQSGIQVTAVEINGAFLSY
                     PFIEEMISQYLDMYSRIDGIFTEDVEAYCCLMQAKKRGIYVPRDLKIVGYDGNEMTRM
                     IDPQVTTIAQDVPLLARTCVEVLAKKIAKEKVDAHYYIPVKLLQGGTTV"
     gene            complement(1287..2642)
                     /locus_tag="LC508_RS12675"
                     /old_locus_tag="LC508_12675"
     CDS             complement(1287..2642)
                     /locus_tag="LC508_RS12675"
                     /old_locus_tag="LC508_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296882.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_033139388.1"
                     /translation="MATEMKKISLFEKLIYGSGDVGLNAMYTLFSSYVLMFYTDVLMI
                     NAALIGTVIMISKIFDGISDLIAGQLIDKHKNKYGHCIPVLMKWAIPMVLSVILVFTV
                     PDGSVGLRVAYIFITYNLFNTILYTYVGMAHGSLASYVTNDSKDRSQMLIFKMMFAAA
                     TQTIMASVMIPMVEYFGGQQMQSAWIKAIMVFGVIGLIPIALNVFFVKERVENDAPAE
                     NILIGVKCAVVNKYWWMAFIVNLMANFILTFNLSVSLYYLKDVVGNIALMGTFVACSN
                     LPGVIISAVAPFLLNKFTKQQMVIFGAGCMLAAQIAFIFMPFTTTMLFITALLRGIGM
                     GFAMGMAGALIGDTIDYGEWKTGVRVQSVLFSAASVGAKVGQGALTAAFGFVLSAIAY
                     DGSLAVQTAGTISGIDAFFKYGPLVVAIILLIDILMLDVEKKNPQFIREITERKAGKN
                     A"
     gene            complement(2791..3699)
                     /locus_tag="LC508_RS12680"
                     /old_locus_tag="LC508_12680"
     CDS             complement(2791..3699)
                     /locus_tag="LC508_RS12680"
                     /old_locus_tag="LC508_12680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597727.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_054352228.1"
                     /translation="MEVKEWTYEEFPEFAEEVEGAERIPTTGDEIGVFYQHDVEYAEI
                     DGEKLHLQILRPLTRNRPNMICPCVVFVQGSAWFKQDVYIQLPRVAKLAERGYVVAVV
                     EYRHSGIAPFPAQIMDTRNAVRFMRVHAEEYGIDPEKIVVAGDSSGGHTAMFAGMIHD
                     DVENNLFPGISAEVKGILNFYGSCSVMKEDSNPTQLLHCQPDSPEGMVMGHVDLRESE
                     ELRRRLSVECNIEKDSVIPPTIIFHGTKDRVVNCEGSVILYRRMKECGKDVKLYLIEG
                     ADHSGNEFWMEQVLDIMEDFIQKAVR"
     gene            complement(3943..6417)
                     /locus_tag="LC508_RS12685"
                     /old_locus_tag="LC508_12685"
     CDS             complement(3943..6417)
                     /locus_tag="LC508_RS12685"
                     /old_locus_tag="LC508_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013400.5"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH39 family glycosyl hydrolase"
                     /protein_id="WP_103731126.1"
                     /translation="MDEIRFEFDCDTSVPHFHGELEILYVLTGRCAVMTAKNNYLLSS
                     EDFAVYNPYEHHEIYREAGAHTVSAYISLPILQQANLGRVKCCSKLQPDQADYLSMIR
                     MNLAVVFKEVTDSPDLRRLQILSHLFGLLAILKSQFELPESYELHPTQEIERIQKVLI
                     YIGQHFSEELSLQTVSDLVHLSKSQLSRDFHKILRISFSDYLRQLRLNKAEYLLRTKR
                     QSVTDIALECGFSNTNTFILNFRQQYHITPGNYRRCYEKQASPQPQEGIAAISGTLSY
                     MNLLKYAAKEEAVLPLNKRSTLPTRIEIDARENLGKLQLHHNDSTSLGWAEQLLHEEV
                     RDAVRDSVKTIGFQSVLFFGIFDDSLDFYHEDKNGRPWLSFTYIDLILDFLVDLNITL
                     QIQLGYTPKLLTKDRQHVFGNSCIGLPDNLEKWKFLVSGFLEHLVYRYGIELVSLWSF
                     TPEQLLFVQYGVFSLEDYLYYYETTWKILQKQLPGAMWKARVFDIDFLSVDGFDILYR
                     FLDYCRQKSCLPTQLSVQSFCMDHAKYDRAVTENRIQAKMDQQPGEPAPISADESVLQ
                     HRLQKLRSCLDEYGYSDLPISVFGWNTTLWQNDLGNDTCFKAAAVVKHCLESRNLVTA
                     MAMAHLTDNSERRVMNSNPYHGGYGILNYYGIPKAAYYAMMFLTLLKNNGDIILAEGD
                     GYIVTRSGEGRKLQILLYHYCHYDEQNHLTEWIPLEEQRTIDRYMGFVQKGTRSFAIH
                     LQNMIPGNYLKRSFSVNREHGSSYDQWMTMGAPDLQNRTQKLYLTKISTYGALYENVL
                     IGKSGSFNLSTVLDEHEVRLILLEKR"
     gene            complement(6523..7881)
                     /locus_tag="LC508_RS12690"
                     /old_locus_tag="LC508_12690"
     CDS             complement(6523..7881)
                     /locus_tag="LC508_RS12690"
                     /old_locus_tag="LC508_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="WP_103731125.1"
                     /translation="MAGKNYYDVTEWNVGDPYKDIGEVINSMLADIKSRQTDSNVEEG
                     GKPGAVIYIPSGDYHLRTQVVIDISYLKIMGAGHGFVSSSIRYNLPKSEWEDLHEVWP
                     GGSRILVDLSPKDADGEAAGAAFYVERSGNPRISSVEFENFCIDGLHFADDGSGKNDP
                     ENTYVNGKTGIYVASAQDSFRITGMGFVYLEHGVIIYHADALSVHDNFIAECGNCIEL
                     RGWGQASKITDNLMGAGYRGYSVFAQNFGGLLITSNNIFPRGTSCVHFDKVARSVITG
                     NRFHSFYPGALVFEGNCSENTVSSNHFLRDHEPWAPMLKNDNGLDDRYGLLRISGNHN
                     SVIANHISETIHRQSIKPAGAKPVIIHVVSGKGNYISDNHIVATTEAADAQTGASDSC
                     FSAQVDALTATDKLAVLDVTTVLVAKESFQNTILDSGSEEQVVLDRRVNAFRATPVPG
                     GV"
     gene            complement(7941..9425)
                     /locus_tag="LC508_RS12695"
                     /old_locus_tag="LC508_12695"
     CDS             complement(7941..9425)
                     /locus_tag="LC508_RS12695"
                     /old_locus_tag="LC508_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_103731124.1"
                     /translation="MSKEILKLNIEKAQMETDRKKEDVKNGVMRQRYHFMPQTGWLND
                     PNGLIYFRGKYHFFFQHNPYSGFWDSMHWGHAISEDMLHWEYLPPALAPSEVYDDHPK
                     GGCFSGSAIEHDNKLFLMYTGTANKGRGYEQTQCIAYSEDGIHFEKYEGNPVLTAPEG
                     ILPDMFRDPKVWKHEDMYYMVCGASRENRGLALLYRSGDMLHWKFFQVLAESRGEWGY
                     MWECPDFYPIGDKYVLTFSPMGAGDHTAVYLVGDFDYKTGRFYPHVSGEINWGFDYYA
                     PQSFPVPDGRRIMAGWSNEWEWMPLWKDWGPTYREGWCGFFNIPLEVRLMEDSTLQFL
                     PIEEIKALRQNPEYLDSLVITEEETELRAGDGVAFELKLKIDLENTDADKLELLLRCG
                     EGKKTVCLFDLENAEMSVDREKADGWSRGISRSVMYLKGKRELDVHILSDRSSLEIFT
                     DNYRNNHSNNIFAGETQNQLKIRAIGGSAVIKEIETYGIKDCYC"
     gene            complement(9444..10274)
                     /locus_tag="LC508_RS12700"
                     /old_locus_tag="LC508_12700"
     CDS             complement(9444..10274)
                     /locus_tag="LC508_RS12700"
                     /old_locus_tag="LC508_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341277.1"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_103731123.1"
                     /translation="MKFKGEVGKKLGISLSVVLTVFWLFPYIYVICCSFKPGSEVIAV
                     PPSFFPKVFSVENFGGLFERMDALGFLFNSLSASLISTVIALILGSLAAYAIQRSGAK
                     LSIILVVLVLCLKMIPTSSIVVPIYELICNLGLYDTKIALIIVYAAINMPFVMWTMLS
                     FYEGIPTTLDEAAFVDGAGSFQTFLKVILPICKPGLATAFIFTLFLAWNDFLVALLLT
                     STGAKTFTVGLAGFLSAYNLDLGPMCAGAFLFSFPVMIISLAAQKYIVQGMTAGALKG
                     "
     gene            complement(10300..11184)
                     /locus_tag="LC508_RS12705"
                     /old_locus_tag="LC508_12705"
     CDS             complement(10300..11184)
                     /locus_tag="LC508_RS12705"
                     /old_locus_tag="LC508_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221152.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_033139383.1"
                     /translation="MRLISRKTLLLFFLPGAVFMGAFLIYPIITMVADSFFQIGITGE
                     KTFIGLENYIRAFTAGGFLKQLKNTLIYIAVAVSFETAIGLLFALFFELNYKGSKVIR
                     SFMMAPLMIAPLVAGLTWKLMMSSSFGIVNELLAKIGILSSSSDILWLADERWSLAAC
                     CIADIWLTTPFMMLMILAGLQGLDSSMLEAAKIDGAGMLQQVFAIKLPVIKPVLLTAL
                     SVRIIDAARTFDIIWAMTEGGPNSSSETLSIIIYKTLTRYNDVGYASAMAVVFIAVLV
                     IFTLVFMQSLWNPKKKNC"
     gene            complement(11303..12529)
                     /locus_tag="LC508_RS12710"
                     /old_locus_tag="LC508_12710"
     CDS             complement(11303..12529)
                     /locus_tag="LC508_RS12710"
                     /old_locus_tag="LC508_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221156.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_103731122.1"
                     /translation="MKKKMLSIILAAMMAGTMATGCGADGGKKEENHKNSITVLVESG
                     SPAEALAKETAASFKEETGCEVIIDAVAYTGMYDKLSTEIKAKQAAHDVACMDVVWLA
                     AFADAVEAVNDADTSDFLPTLQESGTIDGNLLGYPMWVNTKVLIYRKDLIPEEKVPKT
                     WEEYQAVAEEMKTDDMSGTTVFGSGSDAVCSFLDFACQAGAKGLVFDKDGNVNITDQA
                     YADALHFMVENANADYTPSDSLATASTESQELFTNGKTAMQLNWSHQYPAAVKALGAD
                     KVGCTPMISGSAGIGATTGPWYECVMKGSENKEMAKKYVEYMYSHNADYMDLTLKIAG
                     RTSVYEEAGKEAGNEHTAAVLDTLKAKQSQARPMVTTWAQIEEVLIGVVEASLGGADI
                     NETLESAKNEIEAIAR"
     gene            12897..13877
                     /locus_tag="LC508_RS12715"
                     /old_locus_tag="LC508_12715"
     CDS             12897..13877
                     /locus_tag="LC508_RS12715"
                     /old_locus_tag="LC508_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221158.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_033139381.1"
                     /translation="MSKVKITDIAERSGVSLTTVSRYFNHPELLSQETQDKIKAAIKE
                     LNYQQDNLARILVTGESNLVGVIFPHLHLSFYTELLNQLIKYGKEKGYNLIVYTSNDS
                     REEELQLIQNLISYRIKGLILLSHLLSAEDIEKLPVPVISVERVGGNFKQINNDNFTG
                     GKLAANLLVRNGCEVFIHINNDFREDWPSFKRILGFEYEVRDLCYERVIEHDFTDTYT
                     LKAAESMNRLVQHIITKYAGKKIGVFCSNDDIASLFERQCIKNGIAIPNTIELVGYDN
                     SPVSNYAVHSITSIDQNIPLMAQIAIQSLENYIPYESMVPATLVEKETTS"
ORIGIN      
        1 tcacacagta gttccaccct gcaataattt caccggaata taatagtggg catctacttt
       61 ttcttttgcg atcttcttcg caagaacctc cacacaggtg cgtgccagaa gaggtacatc
      121 ctgggctatg gtagttacct gcggatcgat catccgcgtc atttcattgc cgtcataccc
      181 cacaatcttc aaatcccttg gcacatagat tcctcttttt tttgcctgca tcagacagca
      241 gtatgcttcc acatcctctg taaaaatacc gtcaatccgg ctgtacatat ccagatactg
      301 gcttatcatc tcttcaataa acggatagga caaaaatgcc ccgttgatct ccacagctgt
      361 aacctgtatt cccgattgtt ccagtatccg tctgcactcc ttaatcctgt tctccgcatg
      421 tacttttgtg gcaaacctgg cccccaggat cataacattt ttgcacccgt tttctttcag
      481 cagctttgcc gcaatctgac caccctttat atgatcagag gcaaccatgg gtatttcttt
      541 tgccagcacc cgctcaaaac tgactaccgg catgcccttc attctctcga tttcctctcg
      601 cgttacatct gtattgatga tcatgccgtc aagactgccc gcctcaatca aatccagcgt
      661 atccgaaact ctcttctgaa ctcccagcgt attgcacact aaagttttat aaccaatatg
      721 attcaattcc agttctgcat actttaaaaa ggccccatag aacggaaagg acacatccgg
      781 cagtacaatc ccaaccagtc cgctcttttt ctgaacaaaa ctacgtgaca gctcactggg
      841 tctataattt aactcttttg cgattctcct gattttctct tttttaccgg cttccacata
      901 accgctgtca ttcagcgccc tggataccgt tccgatcccc acgcctgcag ctctcgccac
      961 atctcttata tttgccatgc tgacctctta tttgactttt ttaataccca ttccatctat
     1021 ttaacaacac gggctatgtt tacaatatca tacatttttt agtaatgtca aactattata
     1081 agttcttcca gaaccagctg aatcccaata ggctgtttga attttcttgc tcattcaatt
     1141 aagcataaaa attatttatt aaataacttg gctgtctggg caactgccgc gagtatttag
     1201 tgataattac cttataacat gtaacggaga gaagacgcgt ggaaaccaca acctattatg
     1261 taactatata ccattacgag acaagtttag gcgtttttac ctgctttacg ttctgtaatt
     1321 tccctgataa actgcggatt cttcttttca acatccagca taagaatatc aatcaaaagg
     1381 ataatggcaa caaccagcgg accgtattta aagaatgcat ctataccgga aatggtgcct
     1441 gcagtctgaa ctgccaggga accgtcataa gcgatagctg agaggacgaa accgaatgcc
     1501 gcggtcagcg caccctggcc gactttcgcc ccaactgaag ctgcggagaa gagaacggac
     1561 tgaacgcgca ctcctgtttt ccattcaccg taatcgatgg tatcaccaat caaagcacct
     1621 gccattccca tcgcaaagcc cataccgata ccgcgaagca atgcggtgat gaaaagcatg
     1681 gtcgtcgtaa acggcatgaa gataaatgca atttgtgcag caagcataca tcctgcgcca
     1741 aaaatgacca tctgctgttt cgtaaattta ttgagcagaa acggggcaac tgctgaaatg
     1801 atcacacccg gaagattgga acaggcaaca aacgtgccca tcaaagcgat attgccaaca
     1861 acgtccttca aatagtacaa ggataccgaa aggttgaaag tcaggataaa gtttgccatc
     1921 aaattgacaa taaacgccat ccaccagtat ttattaacaa ctgcacattt cacaccaatc
     1981 aaaatgtttt ctgccggagc gtcattttcc actcgctctt tcacaaaaaa gacgttcagc
     2041 gcaatcggaa tcaaaccgat aacaccgaat accatgatgg ctttgatcca tgcagactgc
     2101 atctgctgac cgccaaagta ttccaccatg ggaatcataa ccgatgccat gatggtctgg
     2161 gtagcggctg caaacatcat cttaaaaatg agcatctggg aacggtcctt ggaatcattc
     2221 gtgacatacg atgcaagcga cccatgggcc ataccgacat aagtgtataa tatcgtgtta
     2281 aaaaggttgt aagttataaa aatgtaagca acacgaagcc ctacacttcc atccggcacg
     2341 gtaaatacca gaataacgga aagtaccatc ggaattgccc atttcatcaa aaccggaata
     2401 cagtgaccgt atttattttt gtgtttgtca atcaactgac ctgcgatcaa atcagaaata
     2461 ccgtcgaaaa tttttgaaat catgatcacg gtaccgatca atgcagcatt tatcatcaat
     2521 acgtccgtat agaacattaa aacataggag ctgaacaaag tatacattgc attcagaccc
     2581 acatcaccgg atccatagat cagtttttca aataagctga tttttttcat ttctgtagcc
     2641 atttcaatat ccttctttcc tttttatagg tccctcagta taacagttca gacaggtctg
     2701 tgcatttact gcgctgaccg tatgaaaacg taccggagac agacatccag cccatcgtga
     2761 agcgattctt aatgtctgga tgtattctga ctatcgcact gccttttgga tgaagtcctc
     2821 catgatgtcc agaacctgtt ccatccagaa ttcgttgccg ctgtggtccg ctccttctat
     2881 caaatacagc tttacatcct ttccgcattc tttcatgcgc cgataaagaa tcacgctgcc
     2941 ttcacagttt acaacacggt cttttgtacc atgaaatatg atcgtcggcg ggatcacact
     3001 gtctttctct atgttgcatt ccacagaaag cctgcggcgc agctcctcgc tttcgcgcag
     3061 gtccacatgc cccatcacca taccttccgg actgtccggc tgacagtgga gaagctgggt
     3121 tggatttgaa tcttccttca tgacactgca gcttccgtag aaattcagaa tgcccttaac
     3181 ctccgcagaa atacccggga agagattatt ttccacatca tcgtggatca tgccggcaaa
     3241 catggcagtg tgcccaccgg aagagtcgcc tgcaacaacg attttttcag gatcgatccc
     3301 gtattcttcc gcatgtacgc gcataaagcg tacggcattt ctggtatcca taatttgtgc
     3361 cgggaaaggt gcaatgcctg aatgacgata ttccaccaca gccactacat aaccacgttc
     3421 cgcaagcttt gccactcgcg gaagctgtat atatacgtcc tgcttaaacc atgcggaacc
     3481 ctgtacaaac actacgcatg gacagatcat gttcggccgg tttcgggtca gcgggcgtaa
     3541 aatctgcaga tgcagttttt caccgtcaat ctccgcgtac tccacatcat gttgataaaa
     3601 aactccgatt tcgtcgccgg tggtcggaat cctttccgct ccttccacct cttctgcaaa
     3661 ttccggaaac tcttcatagg tccattcttt tacttccata agatcacctc gttcagttat
     3721 tgttttacaa attgtatcat acgttttgcc cggctttctc ttgattcctc cgcaggtttt
     3781 attcattttt cacccacatt ttttttaaaa atgacattca cagatctttt ttcatgaaat
     3841 aaatcgtgat ttattgtgct gtaaatcgtg atttttgaca gcaaaaaggg aaatgccgaa
     3901 catttcagca tttcccacta aataatcact ttgctatttt ccttaacgct tttccagcaa
     3961 tatcagccgc acttcatgtt catccagtac ggtggacagg ttgaagctcc cgctttttcc
     4021 gatcaggaca ttttcataaa gagcgccata ggtactgatc ttcgtcagat acagcttttg
     4081 tgtacggttc tgcaaatccg gcgcgcccat tgtcatccat tggtcataac tgctcccatg
     4141 ctcacggttt acggaaaagc tgcgtttcaa ataattgcct gggatcatgt tttgcagatg
     4201 aatggcaaag ctgcgagttc ctttctgcac aaatcccata tagcggtcga ttgttcgctg
     4261 ttcctccagc ggaatccatt ccgtcagatg attttgttca tcatagtggc agtaatggta
     4321 cagcagaatc tgcagtttcc tgccctcccc ggaacgagta actatatatc cgtccccctc
     4381 agcaagaatt atatcgccgt tgttctttaa aagcgtcaga aacatcatgg cataataggc
     4441 cgcctttggt atcccgtaat agtttaaaat accataaccg ccatgatagg gattagagtt
     4501 catgacacgg cgttccgagt tatctgtcaa atgtgccatt gccattgcag tgaccagatt
     4561 gcggctctcc aggcagtgct tcactactgc ggctgcctta aaacaggtat cattccccaa
     4621 atcattctgc cacaatgtgg tattccaacc gaaaacagag atcggcagat ccgaataccc
     4681 atactcatcc agacaagaac ggagtttctg cagacggtgc tgcagtacac tttcatcagc
     4741 actgatcggc gccggctccc ccggctgttg gtccatcttt gcctgaatac gattctcggt
     4801 gacggcacgg tcatatttgg catgatccat gcagaaactc tgtacactca gctgggtggg
     4861 aaggcagctc ttttgtctgc agtagtctaa aaagcgataa agaatgtcaa atccatccac
     4921 agataaaaaa tcaatatcga acacacgtgc cttccacatg gcccccggta gctgcttctg
     4981 caatatcttc caggtggttt cgtaataata aagatagtcc tccaaggaga aaacaccata
     5041 ctgtacaaac aggagctgtt ccggtgtgaa actccacagc gaaaccaact ctattccata
     5101 acggtaaaca agatgctcta aaaaaccact cactaaaaat ttccactttt ccagattatc
     5161 cggaagccca atacaagaat tcccaaaaac atgctgacga tctttggtca aaagttttgg
     5221 ggtataaccc agttggatct gcagggtaat attaagatcc accaaaaaat ccagaattaa
     5281 atcaatatag gtaaaactca gccatggcct gccgtttttg tcctcatgat aaaagtccag
     5341 agaatcgtca aaaatcccga aaaacaatac actctgaaag ccgattgttt tcacagaatc
     5401 gcggactgca tcacggacct cctcgtgcaa gagctgttct gcccagccaa gtgacgtgga
     5461 atcgttgtga tgaagctgca atttaccaag attttcccgg gcatcaattt caatacgggt
     5521 tggcagagtg ctgcgtttgt ttaacggcag cactgcttct tcttttgccg catattttaa
     5581 aagattcata taagacagtg taccggaaat agcggcgatt ccctcctgcg gctgcgggga
     5641 tgcctgtttt tcataacatc ggcgatagtt tccgggcgta atgtggtatt gctgccgaaa
     5701 gttcaatata aaggtgttgg tattggagaa accgcattcc aatgcaatgt ccgtcacgct
     5761 ctgccgtttc gtgcgcaaaa gatactctgc tttatttaag cgcagctgac gcagataatc
     5821 tgaaaatgag atgcgcagta ttttatgaaa atcacgggac aactggcttt tggagagatg
     5881 caccagatcc gaaaccgtct gtaaactcaa ttcctctgaa aaatgctgtc cgatatagat
     5941 caggactttc tgtattcgtt ctatttcctg tgtcgggtgc agttcgtatg actccggtaa
     6001 ctcaaactgt gatttcagga tagccagcag tccgaaaagg tgacttaaga tctgcagccg
     6061 gcgcagatca ggcgaatcgg taacctcttt gaacaccacc gccaggttca tgcgaatcat
     6121 ggaaagataa tcggcttgat ccggctgcaa tttactgcag catttaacac gtcctaaatt
     6181 tgcctgctgt aaaatcggca gcgaaatata tgccgaaaca gtatgcgcac ccgcttcgcg
     6241 ataaatctca tggtgttcat acgggttata tacagcaaag tcctcactgc tcaaaagata
     6301 attgtttttt gccgtcatca cagcacaccg gccggtcagc acataaagta tttccagctc
     6361 cccatgaaaa tgcggaacgg atgtgtcgca gtcaaattca aaacgaattt catccatgtg
     6421 aaatcacccc ttttttaatt ataccgataa aaaaggtatc tgtcgaattc tttttcagat
     6481 tgtaggcgat atcacagaaa ggaccatttg tcctgtcccc ggtcacactc ccccaggcac
     6541 cggcgtagcc ctaaaagcat tcacacgcct gtcaaggaca acctgctcct cacttcctga
     6601 atccagtata gtattttgaa aggattcttt tgccaccaga acagttgtca catcaagcac
     6661 cgccagttta tccgtagcag taagagcatc cacctgtgca gaaaagcagg aatccgatgc
     6721 cccggtctgt gcatctgctg cctccgttgt ggcaacgata tgattatcag atatataatt
     6781 tcccttgccc gagacaacat gtatgatcac aggttttgcg ccggccggct tgatagactg
     6841 cctatggatc gtttctgaaa tatggttggc gatcaccgaa ttatgattgc cgctgatgcg
     6901 cagaaggcca tacctgtcat ctaagccatt gtcattttta agcataggcg cccatggttc
     6961 atgatcacgt aaaaagtggt tggaagagac cgtgttttcc gaacagttcc cttcaaatac
     7021 cagtgccccc ggataaaagg aatgaaatct gttgcctgtg ataacagagc gggcaacctt
     7081 atcgaaatgg acacagcttg tacctctcgg aaaaatatta tttgaagtga tcaaaagtcc
     7141 tccgaaattc tgcgcaaaaa cagaatatcc cctgtacccg gctcccatca ggttatcagt
     7201 tatcttagat gcctgtcccc agccgcgcag ttcgatacag tttccgcatt cagctataaa
     7261 attatcatgg actgacaatg cgtctgcatg gtaaataata accccatgct ctaaatatac
     7321 aaagcccatg cctgtgatcc ggaaggaatc ctgtgcactg gcaacataaa tgcctgtttt
     7381 gccattcaca tatgtatttt ccggatcatt ttttcctgaa ccgtcgtccg caaagtgtaa
     7441 tccgtcaata cagaaatttt caaattccac agagctgatc ctgggatttc cgctccgttc
     7501 cacataaaag gcggctcctg ccgcctcacc atctgcgtcc ttcggagaaa ggtcaactag
     7561 tatgcggctg cctcccggcc acacttcatg cagatcctcc cattcactct ttggaagatt
     7621 ataacgaatg cttgaagata caaaaccatg tccggcaccc atgattttca aataactgat
     7681 atcaatcacc acctgcgtac gaagatgata atcccctgac ggaatgtaga tgacagcccc
     7741 cggttttccg ccctcctcca cattggaatc tgtctgtctg cttttaatat ccgccaacat
     7801 gctgttgatc acttctccta tatctttata gggatctccg acgttccatt ccgtaacatc
     7861 atagtaattt ttacctgcca tacataaatc ctcctatata tttaacgccg cccggcctga
     7921 caataatgct tccatccgtt ctaacaataa caatccttta ttccgtatgt ctcaatctct
     7981 ttgatcaccg cactcccgcc aatcgcccgg attttcaact gattctgcgt ctctcccgca
     8041 aaaatattat tagaatggtt attccggtaa ttatctgtga aaatttcaag tgaactccgg
     8101 tctgacagaa tatggacgtc cagctccctt tttcccttca gatacataac gcttctcgaa
     8161 atacctctgc tccatccatc tgctttctca cgatctacac tcatttccgc attctcaaga
     8221 tcaaacagac ataccgtctt ttttccttct ccgcagcgca aaagcagctc cagtttatcc
     8281 gcatctgtat tctcaagatc aatcttcagc ttcagttcaa aagccacacc gtctccggct
     8341 ctcagttctg tctcctcttc cgtgatcaca agactgtcca gatattccgg gttctgcctc
     8401 aaagctttta tttcctcgat tggcagaaac tgtaacgtgc tgtcttccat caaacgtacc
     8461 tccagaggga tgttaaaaaa gccgcaccag ccttccctgt aggttggccc ccagtccttc
     8521 cataatggca tccattccca ttcatttgac catcctgcca tgatccttct tccatccggt
     8581 accggaaatg actgcggtgc ataataatca aatccccagt tgatttcacc gctcacatga
     8641 ggataaaacc ttcctgtctt ataatcaaaa tcccccacca gatacacggc agtatggtcg
     8701 cctgccccca taggtgaaaa ggtcagaaca tatttatcgc ctatcggata aaaatccggg
     8761 cattcccaca tgtatcccca ttccccacgg ctttccgcca gaacctggaa gaacttccag
     8821 tggagcatat ccccggaacg gtagagaagt gccaagcccc tgttttcacg gcttgcgccg
     8881 cataccatat aatacatgtc ctcgtgcttc cataccttcg ggtcacgaaa catatccggc
     8941 aaaatccctt ccggcgctgt cagcaccggg ttcccttcat atttctcaaa atggattcca
     9001 tcctcactgt atgcgataca ctgtgtctgc tcataacctc ttcccttatt ggctgttccg
     9061 gtgtacatga ggaaaagttt attgtcatgt tctatcgcac ttccggaaaa acatccgcct
     9121 tttggatgat cgtcatacac ttcactgggt gccagtgccg gcggcagata ctcccaatgg
     9181 agcatatcct cgctgatggc atgcccccaa tgcatactgt cccagaaacc actataggga
     9241 ttgtgctgaa aaaagaaatg gtattttccc ctgaaataaa tcaatccatt gggatcattc
     9301 agccaccctg tctgcggcat aaaatgatag cgctgacgca tcacgccatt cttaacatct
     9361 tccttttttc tgtctgtctc catctgagcc ttttcaatat tcagcttcaa tatttcttta
     9421 ctcataagcc tcctccgtac tccttaacct tttaaagctc ctgctgtcat tccctgcaca
     9481 atatattttt gagccgcaag ggaaatgatc atcaccggaa aactaaacaa aaaagctcct
     9541 gcacacattg gccccagatc caaattgtat gccgacaaaa atccggccag tccaaccgta
     9601 aatgtctttg caccggtact ggtcagcaat aatgccacaa ggaaatcatt ccatgcaagg
     9661 aacagggtaa atataaacgc tgttgccaaa ccgggtttac agatcggcag aataaccttc
     9721 aaaaatgttt ggaagctgcc tgccccgtct acgaatgctg cctcgtccag tgtcgtggga
     9781 attccctcat aaaaacttaa catggtccac atgacaaacg gcatattgat ggctgcgtaa
     9841 acaatgatca aggcaatttt ggtatcatac aggccaagat tacagatcaa ttcataaatc
     9901 ggcactacaa tacttgaagt aggtatcatc ttcaggcata aaaccaaaac caccagtatg
     9961 atcgataatt tggcacctga gcgctgtatt gcatacgctg caagagatcc caatatcaac
    10021 gcgatcactg tacttatcaa ggaagcactc aaactgttga acagaaatcc gagtgcatcc
    10081 attctttcaa acagtccccc aaaattttca accgagaaca cttttgggaa aaaactcggc
    10141 ggaactgcga tcacttctga tcccggcttg aaagaacaac aaattacata tatgtaaggg
    10201 aaaagccaga atacagttaa aaccactgac agactgattc ccagcttctt cccgacctca
    10261 cctttaaatt tcaccttttt tcgcctccta tccactcagc taacagttct ttttcttcgg
    10321 attccacaga ctctgcataa ataccagcgt aaatattact aatactgcaa taaataccac
    10381 tgccattgcg ctggcatacc ccacatcatt atagcgcgtc aatgtcttgt atataataat
    10441 actgagtgtt tccgatgagc tgttcggacc tccctccgtc attgcccaga taatatcaaa
    10501 ggttctggcc gcgtcaatga tccttacgga caatgctgtc agtaaaaccg gtttgatcac
    10561 aggaagcttt atggcaaaga cctgctgcag catacctgcc ccgtcaattt tcgcggcctc
    10621 cagcatactg gaatccaggc cctgcagccc ggccaatatc atcagcatca taaacggtgt
    10681 agtgagccaa atatcagcga tacagcatgc agctaacgac cacctttcat ccgcaagcca
    10741 cagaatgtca ctgctgcttg acaaaatccc aattttagcc aatagttcat tgacaattcc
    10801 gaaactggaa ctcatcatca atttccatgt aagaccggct actaaaggcg cgatcatgag
    10861 gggtgccatc atgaaggatc gtatcacttt acttccttta tagttcaatt caaagaataa
    10921 tgcgaataat aaaccgattg ccgtctcaaa acttacagcc actgcaatat atatcaaagt
    10981 attctttaat tgttttaaaa atccgccggc ggtaaatgca cgtatatagt tttccaaacc
    11041 gataaatgtc ttctcacctg tgattccgat ttgaaagaaa ctgtcggcta ccattgtaat
    11101 gattggatat atgagaaatg cccccataaa taccgcaccc ggcagaaaaa atagtaataa
    11161 tgtttttctt gaaatcaatc tcattctatt taccaccttc tataaacaga ggggaaaaat
    11221 cgctgttcac tccgtgccca gcaacacgct tcgcgggatt cccctctgct gctcacttta
    11281 tctgtttcac tgcgcgcctc cgttatcggg cgatcgcttc aatctcattc tttgctgatt
    11341 ctaatgtctc gtttatatct gcgccgccca gactggcttc cacaacaccg attaaaacct
    11401 cttcgatctg agcccatgtt gtcaccatag gccttgcctg tgactgcttt gcttttaacg
    11461 tatcaagaac tgccgctgta tgctcattgc ccgcctcttt acctgcttct tcatatacag
    11521 atgttctgcc tgcgatcttt aaggtaagat ccatataatc cgcattgtgg ctgtacatat
    11581 attctacata ttttttagcc atctctttat tttcagagcc tttcataacg cattcatacc
    11641 agggaccggt tgttgcccca atacccgcac ttccgctgat catcggtgtg catccgactt
    11701 tatcggcacc gagagccttt actgccgccg gatactgatg gctccagttc agctgcatgg
    11761 ctgtttttcc gttcgtaaag agttcctgcg actctgtaga ggctgtggca agtgaatctg
    11821 acggagtgta atcagcgttg gcattttcca ccataaaatg caatgcatct gcgtaagcct
    11881 gatctgttat atttacattg ccgtctttgt caaatacaag tccctttgca ccggcctgac
    11941 atgcaaagtc aagaaaagaa cacactgcat ccgatccact tccaaagact gttgttccgc
    12001 tcatatcatc ggtcttcatt tcttccgcaa ctgcctgata ctcttcccat gtctttggca
    12061 ctttttcttc cggtataagg tcttttctgt agatcagcac ttttgtattt acccacatcg
    12121 ggtaccccaa aagattccca tcaatcgttc cgctttcctg taatgtaggc agaaaatcac
    12181 tggtatctgc atcattgacg gcttccactg cgtctgcaaa tgctgccagc cataccacat
    12241 ccatacacgc cacgtcatgg gccgcctgct ttgccttgat ctctgtagaa agcttatcgt
    12301 acattcctgt ataggcaacc gcgtcaataa taacctcgca tcctgtctcc tctttaaaag
    12361 atgccgctgt ttcttttgca agcgcctctg cgggtgaacc gctttctacc agtacagtaa
    12421 tgctgttctt atgattctct tccttttttc ctccatctgc cccgcacccg gttgccattg
    12481 ttcctgccat cattgccgca agtatgatac taagcatctt tttcttcatt tttcaatcct
    12541 cctgttttcc tattgatcat ctgttttcgt tacttttgct ttgatggctc tattgtattc
    12601 catgaaactt ataatgtcaa acgttttcat tactaatttt cattttcttt caattttcac
    12661 aagatatttc ccttattttt atctatattc cataatacct tacccgattt tcatcgaatt
    12721 tatcttcaaa tgataaaaca tatatgaaat ttttttcatg aaattatttt cattttccct
    12781 ttcatttttc ataatactgt gctataattt ttgctaaaca gtgagcattt ataactattc
    12841 cgtatgtctg cgcttttgct gcgctgaccg taaaaaacga tataagtgag gtttttatga
    12901 gtaaagtaaa gattacagat atcgcagaga gaagcggcgt ttccctgact accgtttccc
    12961 ggtatttcaa tcatcctgaa cttttgtctc aggagacaca agataaaatc aaagctgcga
    13021 tcaaagaatt gaattaccag caggacaatc tggcccgtat tttagtaacc ggggaatcca
    13081 atctggtagg agtcattttt ccccatcttc atttaagttt ctataccgag ctgctaaatc
    13141 agcttattaa gtacggcaaa gaaaaggggt ataatttaat cgtctatact tccaacgatt
    13201 caagggagga ggaactgcag ctgatccaaa acctgatttc ctaccggatc aagggattga
    13261 tactgctgag ccatctcctt tcagccgaag acattgaaaa gctgcccgta ccggtgatct
    13321 ccgttgaacg tgtcggcggt aatttcaaac agatcaacaa cgataacttt accggtggaa
    13381 agctggccgc aaatcttctg gtccgcaacg gctgcgaggt tttcattcat atcaataatg
    13441 attttcggga agactggcct tccttcaaga gaattttggg atttgaatat gaggtaagag
    13501 acttatgtta tgaacgtgtg atagaacatg atttcacaga tacttacaca ctaaaagctg
    13561 ccgaatccat gaaccggtta gttcagcata ttatcaccaa atatgccggt aagaaaatag
    13621 gtgtcttttg ttccaacgat gatattgcga gtctgtttga acgccaatgc atcaaaaatg
    13681 ggattgctat tccaaataca attgaactgg tcggatacga caactccccg gtgtccaact
    13741 atgccgtaca ttccatcact tccatcgatc agaatatccc tctcatggca cagattgcaa
    13801 tccagtcact ggaaaactat atcccatatg agagtatggt gcctgctaca ttggttgaaa
    13861 aagaaacgac aagttaa
//
