LOCUS       OZ022674                6361 bp    DNA     linear   ENV 15-MAR-2024
DEFINITION  Uncultured bacterium DNA fragment from in-reactor enriched cow
            rumen, clone ferm-41o1, contig-1.
ACCESSION   OZ022674 REGION: 14403..20763
VERSION     OZ022674.1
DBLINK      BioProject: PRJEB67889
KEYWORDS    ENV.
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
REFERENCE   1
  AUTHORS   Potocki-Veronese,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) INSA Toulouse, Toulouse Biotechnology
            Institute, 135 avenue de Rangueil, 31400 Toulouse, France
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="uncultured bacterium"
                     /mol_type="genomic DNA"
                     /isolation_source="Cow rumen"
                     /db_xref="taxon:77133"
                     /clone="ferm-41o1, contig 1"
                     /environmental_sample
                     /note="AZCL-Barley beta glucan active clone"
     CDS             complement(1..2250)
                     /EC_number="3.2.1.21"
                     /codon_start=1
                     /transl_table=11
                     /product="GH3"
                     /protein_id="CAK9712109.1"
                     /translation="MKVLKIFPACILLASVVSCKPSAEATGEAAVEQRVETLLKEMTL
                     EEKIGQMNQISPPEDVSQIADLIKKGEVGSILNLVDPEAINALQQIAVEETRMKIPLI
                     VGRDVIHGFKTIFPIPLGQAASFNPELVKAGARVAAVESASQGVHWTFAPMIDIARDP
                     RWGRIAESLGEDTYLTSVLGAAMIQGFQGDELSDPTAIAACPKHFVGYGAAESGRDYN
                     STFIPERRLRNVYLPPFQAAVEAGAATFMTSFNDNDGVPSTGNRFILDEVLRKEWNFR
                     GFVVSDWASVSEMISHGFAKDGKEAAMKAINAGVDMEMVSYTYVTYVKELLAEKKISM
                     ESIDNAVRNILRIKIQLGLFENPYVDTSAPSVLYAEEHLQKAKEAAIESAILLKNENG
                     ILPLNESVKSIAVVGPMADAPHDQLGTWVFDGDKNYTQTPLKALQAAYGDQVRILYAP
                     GTAFSRDTRTEGIAAAVNAARNADVVLVFVGEEAILSGEAHSLADLNLQGAQSELIAA
                     VAKTGKPVVTIVMAGRPLTIGRDAALSQAVLYNFHPGTMGGPAIADLLFGKAVPSGKL
                     PVTFPKMVGQIPIYYGHNSTGRPYHGNETLLADIPLEAGQTSLGNTSFHLDAGFAPLY
                     SFGYGLSYTTFDYSNLKISADRLKATDVMTVTADITNTGKYDATEIVQLYVQDCFGSV
                     TRPVKELKDFARVFLKSGETKTVVFSLPIEKLAFFNLDMNYGVEAGDFNLWVGPNSQE
                     GLKTSFVVE"
     CDS             complement(2263..3360)
                     /EC_number="3.2.1.73"
                     /codon_start=1
                     /transl_table=11
                     /product="GH16_3"
                     /protein_id="CAK9712112.1"
                     /translation="MKIKNMIPLLILILASMGCGGSEDTPVVDEKITCSPAAISAGYA
                     GETATIEVVSTREWTAYSNDEWLTCTPSSSIETTGTVTIQVDANPKTAIREGLVIVKA
                     GSSRDTVRVTQAAKPETPVDPNITVPEGYQLVWNDEFESGTAPGAEWWYETGDHGWGN
                     NELQNYVAGSYGSEQLASVKDGILTITAKKIGDTVYSIRMNTGQGWKYGYFEARLKLP
                     TGKGTWPAFWMMPKNYTAWPDDGEIDIMEEVGYNPNYVSSAIHCKAYNHSIGTQKTKD
                     VLIPTAQTDFHVYALEWTEDYIRTYVDGKELFYFANDKTGNKDTWPFNAAFYLKLNLA
                     WGGNWGGAQGVDESVLPATYEIDYVRVFQKK"
     CDS             complement(3434..4798)
                     /codon_start=1
                     /transl_table=11
                     /product="FIG00412289: hypothetical protein"
                     /protein_id="CAK9712117.1"
                     /translation="MKKRYNYAKSILMMLALALGITACSPDDFTSPNEAGIPLAANYE
                     DAITILVNQETNWVTFSFNATGVMPLWIIDGTTYTSTFNMTKYYRKAGDYSVDVKIVN
                     ANGISDGVITKSFTINKTIMNGFGGFVYDSDFNLWKKATIATPTFWYAPGWSQIADPT
                     FKSDGASYSISLPEATTDTWQAQMLMGTDIATNANTHYDFSAILTATQDHPHVMVKLV
                     DSTDDNVFYFAETVKLTANEPVCFWKSDMEGIDIGSLKMVFDFGGNAAGTDITIENIV
                     LKDHANDDGTIVPEEELVPEPNWSAVDSDDNLWKNVTFTNTFYYAPGWSQLADPDLTI
                     EGTQYSVDFPSATTDQWQNQVTFVTDNLSTTSAENYDFKVTLKASNDIQGVTVKLAQN
                     DDDNVFLVMERIDLLAGEDVVAKVINAEGVDITQGKLVFDFGGNPANTHVVIKDIILQ
                     KHKD"
     CDS             complement(4819..6243)
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD domain protein"
                     /protein_id="CAK9712120.1"
                     /translation="MTQETIDTYFTTDEHIQEAVVAAYDPLHWSDWGIGQYNPLCIMS
                     DIMADDIWVGGADKNDNQYWHLMMNYEALPTNCMTGLWTVAYSGVKRSNDVLTYIGWA
                     GDNLTAENATYYEAQARVLRVLYYNWLWKFWGNIPYYEENLTAPYIAEQLSADAVYAN
                     IIADLEGAIALDALPMKASSSDYGRVTKAMAYMLYAEIVLYQNDESRYAKALAYMQEI
                     INSGQYELVDDYTTIFKESGEWNSESIFEVNYKDDNAARSWGSPLVAGGTVLPRLISP
                     SGWADGTDGHDNGWGFCPVRLETYERYSNNDARREATCWNAAAIGSYNTRYQDTGFFL
                     EKYAAKTGDNADQIADADLNYNNNLRIYRFSETLLNAAELIVRGGGSGDAKEYLNRVH
                     KRAGLTIEVAATIDNIIEERHLEFVGEGKRYWDLIRTGKAASVLVPDSYGYRTNAWTE
                     NKKYLPIPQSEIDAAQGTLTQNNY"
     CDS             6242..6361
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAK9712123.1"
                     /translation="MDSTSKNDSEHPVSNKAEAMNPATILFLYLFITIYLRFE"
ORIGIN      
        1 ttattccacc acaaaggatg tttttaaacc ctcctgactg ttggggccta cccacagatt
       61 aaaatcaccg gcttcaacgc cataattcat gtctaaatta aaaaatgcga gcttttctat
      121 aggtaatgag aatacgaccg tcttcgtttc gccggacttc aggaagacac gggcaaagtc
      181 tttcaactct tttacgggac gggtcacgga tccaaaacaa tcttgtacgt acagctgaac
      241 aatttctgtt gcgtcgtact tgcctgtatt tgtgatatcg gcggtaacgg tcatcacgtc
      301 tgtcgctttg agcctgtctg ccgagatttt taaattactg taatcgaacg tcgtatagga
      361 caatccatat ccaaaggaat acagcggagc gaatccagca tccaaatgga aagaggtgtt
      421 ccccaaagag gtttgaccgg cttccaaggg gatatccgcc aacaaggttt cattgccatg
      481 ataggggcgg ccggtgctat tatgtccgta ataaattgga atctgtccga ccatcttcgg
      541 gaatgtgaca ggcaacttcc cactgggtac cgcttttccg aaaaggagat cggctatcgc
      601 cggaccgccc atcgtaccgg gatggaaatt gtataacacc gcctgcgaaa gcgccgcatc
      661 gcgtccgatc gttaagggac gtcccgccat cacaatggtc actaccggct tgccagtctt
      721 ggccaccgcc gcaatcaatt cgctctgtgc accctgcagg ttcaagtctg ccagtgaatg
      781 agcttctccg gaaaggatcg cttcttcacc gacaaagact aaaactacat ccgcgttgcg
      841 ggctgcgttt acggctgctg caatgccctc ggtacgggta tcccgactaa acgccgttcc
      901 cggcgcgtag aggatacgta cctgatcgcc ataagccgcc tgcaaggctt tcaatggagt
      961 ctgcgtgtaa ttcttgtctc catcaaaaac ccatgtgcct agttggtcgt gtggcgcatc
     1021 cgccatcgga ccgactaccg cgattgattt tacagattca ttaagtggta aaatgccgtt
     1081 ttcgtttttt aagaggatgg ccgactctat cgccgcttcc ttggctttct gcagatgctc
     1141 ttctgcatac aaaacggaag gcgccgaggt gtcgacataa ggattctcga acaagcctaa
     1201 ttggattttt atacgtagga tgttgcgtac ggcattgtca atcgactcca tgctgatttt
     1261 cttttcagct agcaactcct tcacgtaggt gacatacgtg taactcacca tttccatatc
     1321 aacaccggca ttgatcgcct tcatggccgc ttcttttccg tctttcgcaa atccgtgcga
     1381 gatcatctcg ctgacagaag cccagtcgga aacaacaaag ccgcggaagt tccattcttt
     1441 gcggagaact tcatccagaa taaagcgatt acccgtggac ggtacgccgt cgttatcgtt
     1501 gaacgaggtc atgaacgtcg ccgcaccggc ttcaaccgca gcttggaatg gcggcaaata
     1561 gacgttgcgc aatcggcgct cagggataaa ggtcgaatta tagtcacgac cactctcggc
     1621 cgcaccgtaa cctacgaaat gcttcggaca agcggcaatg gcagtcggat cgctcagctc
     1681 gtcaccctga aatccctgaa tcatcgctgc tccgagaaca gatgtcaggt aggtatcttc
     1741 gcccaaactt tcggcgattc gtccccaacg cggatcgcgg gcgatgtcaa tcatcggtgc
     1801 gaaagtccaa tgcacacctt gcgaagccga ttcgaccgca gcgacacgtg cacccgcttt
     1861 caccagctca ggattaaacg aggctgcctg acctaacgga atggggaaga tggttttgaa
     1921 accgtgtatc acatcgcgac cgacaatcaa agggatcttc atgcgggtct cttcaaccgc
     1981 aatctgctga agcgcgttga tggcttccgg atcgaccaga ttcaaaatag aaccgacttc
     2041 gcctttcttg atcaggtcgg ctatttggga tacatcttcg ggaggactga tctgattcat
     2101 ctgaccgatc ttttcctcca acgtcatttc tttcaatagg gtttccactc gttgctcgac
     2161 ggcagcttct ccggtcgcct cggcagatgg tttacaggaa accacggatg ccaacaggat
     2221 acaagcggga aaaatcttta acactttcat tttgtttctc tattattttt tctggaatac
     2281 gcgtacatag tctatttcgt aagtggccgg gagaacggat tcatcgactc cctgcgcgcc
     2341 gccccagttg ccgccccaag cgagattgag cttcaagtaa aacgctgcat taaagggcca
     2401 ggtatcttta tttcccgttt tgtcgtttgc aaaatagaac aactctttcc catccacata
     2461 ggtacgtata taatcttcgg tccattccaa ggcgtacaca tgaaaatcgg tttgggcggt
     2521 tggaataagc acatctttcg ttttctgcgt gccaatcgaa tggttatagg ccttgcaatg
     2581 aatggccgat gagacatagt tcggattgta tccgacttct tccatgatat cgatctcgcc
     2641 atcatcgggc caagcggtat agttcttagg catcatccaa aatgccggcc aggtgccctt
     2701 cccggtcggg agttttaagc gggcctcaaa ataaccgtat ttccatcctt ggcctgtgtt
     2761 catacggatg gaatagaccg tgtcgccaat ctttttggcg gtgatagtca gtatgccgtc
     2821 tttcacggac gccaactgct cggaaccata tgatccggct acataattct gcagttcgtt
     2881 attcccccat ccatggtcgc cggtctcgta ccaccactcc gcaccgggag ctgtgccgga
     2941 ctcaaattca tcgttccaga ccagctgata accttcgggc acggtgatat tggggtcgac
     3001 cggcgtttca ggtttcgctg cctgcgtcac ccgaacggta tcacgactac tgccggcttt
     3061 cacaataacc agtccttcgc gtatcgctgt cttgggattg gcatcgacct ggatcgttac
     3121 cgtacccgtc gtttcaatcg aactcgaagg cgtacaagtc agccactcat cattactgta
     3181 ggccgtccat tcacgagtag aaacaacctc aatcgttgct gtttctccgg cgtaaccggc
     3241 gctgatggca gcgggtgaac acgtgatttt ctcgtcaacg acaggtgtgt cttcactacc
     3301 gccacagccc atagaggcaa gtattagaat taataaggga atcatattct ttattttcat
     3361 gattattctc tcgtttgcga atccggatga cctcaagaac gagttttttc tcaaggtcat
     3421 ccttattcat ccattagtct ttatgctttt gcaaaataat gtctttaatg actacatggg
     3481 tattggccgg attccctccg aagtcgaata caagtttgcc ttgcgtaata tcaacccctt
     3541 cggcattgat caccttggct acgacatctt cacctgcgag taaatcgatc cgctccatca
     3601 ccagaaagac gttgtcgtca tcgttctggg ccaattttac ggtcactccc tggatatcgt
     3661 tagaggcttt caacgtcact ttaaaatcgt aattctcagc cgaagtcgtg gaaaggttgt
     3721 ctgttacaaa ggtaacttga ttttgccatt gatcggtcgt tgcactcggg aaatcgaccg
     3781 aatattgtgt gccttcaatc gttaagtccg gatccgccaa ctgtgaccag cccggagcgt
     3841 aatagaacgt atttgtgaag gtgacgttct tccacagatt gtcgtcgctg tcgacagcgc
     3901 tccaattggg ttcgggcacc aattcctctt ccggcacgat ggtgccgtcg tcgttggcat
     3961 gatctttcag gacaatattt tcgatggtga tatcggttcc ggcggcattg cctccaaaat
     4021 cgaataccat tttaagtgaa ccgatatcga tgccttccat gtcgctcttc cagaaacaaa
     4081 ccggttcatt agctgtcaac ttcaccgttt ctgcaaaata gaacacatta tcgtctgtgc
     4141 tgtctacgag tttcaccatg acatggggat gatcctgtgt agcagtcaga atggcggaga
     4201 agtcataatg cgtattggca ttggtagcga tatccgtacc catcagcatc tgtgcctgcc
     4261 aggtatcggt tgttgcttcc ggcagactga ttgaatagga agcgccatcc gatttgaagg
     4321 tcgggtctgc aatctgtgac cagcccggag cgtaccagaa tgtcggcgtg gcaatcgttg
     4381 cctttttcca taagttgaag tccgagtcgt aaacaaatcc gccgaacccg ttcatgatcg
     4441 tcttattgat cgtaaatgat ttggtgatca cgccgtcact gattccgttg gcattgacaa
     4501 tcttcacatc cacactatag tcgcctgcct tacggtaata cttcgtcatg ttgaacgtag
     4561 aggtataggt tgtcccgtca atgatccaga gcggcataac gcccgtcgcg ttgaatgaga
     4621 acgtgaccca gttggtttct tggttgacca ggatcgtgat tgcatcttcg tagttggcag
     4681 ccaatggaat gccggcttca ttcggagaag taaagtcgtc cggagaacaa gccgtaatcc
     4741 ccaaagccaa ggcaagcatc atcaaaatgc ttttggcata attatatcgt tttttcatga
     4801 ttcttctact ttaatagatt aataattgtt ttgagtcaat gtcccctgag ccgcgtcgat
     4861 ttcgctctga ggaatgggca ggtatttttt attctccgtc caagcgttcg tacggtatcc
     4921 gtagctgtcg ggcaccaata cactggccgc ctttccggta cggatcaaat cccagtagcg
     4981 cttaccttca cctacaaact ccaggtgacg ctcttcgatg atattatcga ttgttgcagc
     5041 gacctcgatc gtcagaccgg cccgtttatg aacccgattc aggtattcct tcgcatcacc
     5101 tgaaccgcca ccacgaacaa tcagttccgc cgcattcaaa agcgtttccg aaaaacgata
     5161 gatacgcagg ttattattgt agttcaagtc ggcatccgca atctgatcgg cattatcccc
     5221 cgtcttggct gcatatttct ccaagaagaa tccggtatcc tggtagcggg tattgtaact
     5281 tccgatagct gctgcgttcc agcaggtagc ctcacgacgc gcgtcgttat tggaataccg
     5341 ttcgtaggtt tccaaacgta caggacagaa tccccagccg ttatcgtgac cgtctgttcc
     5401 gtcggcccag ccactcggac tgatcagacg cggtagaacg gttcccccgg ccaccagcgg
     5461 acttccccat gagcgtgccg cattatcatc tttatagttc acttcaaaga tggattcgct
     5521 gttccattcg cccgactcct tgaagatcgt cgtatagtca tcgactaact catattgacc
     5581 gctattgatg atttcctgca tgtatgccaa agctttggca tagcggcttt catcattttg
     5641 gtagaggacg atctcggcgt aaagcatgta agccatggct ttcgtcaccc gaccgtaatc
     5701 cgagctgctc gctttcatag gaagcgcatc caaagcgata gccccttcca aatcggcgat
     5761 gatattcgca taaacagcat ccgcactcaa ttgttccgcg atatagggag cggtcaggtt
     5821 ttcttcgtaa taaggaatat tgccccagaa tttccaaagc cagttataat aaagcacgcg
     5881 aagaaccctt gcctgcgctt cataataggt ggcattttcc gccgtcaggt tatcaccggc
     5941 ccaaccgata taggtcagca cgtcgttgct tcgtttcaca ccggaataag cgaccgtcca
     6001 aaggcccgtc atgcaattcg tcggcagtgc ctcgtaattc atcatcaggt gccagtactg
     6061 attgtcgttc ttatcggcac cacccaccca gatatcatcg gccatgatat cactcataat
     6121 acataacggg ttatattgtc cgatacccca atccgaccaa tgcaaagggt cgtaagcggc
     6181 gactaccgct tcttgaatat gttcatcggt cgtaaaatac gtatcgattg tttcttgcgt
     6241 catggactca acctccaaga atgattcgga acatcccgtc agcaacaagg cggaagccat
     6301 gaatcctgct actatattgt ttttatatct tttcataacg atttatttga gatttgaatg
     6361 a
//
