LOCUS       NZ_CP012938            13877 bp    DNA     linear   CON 08-JAN-2025
DEFINITION  Bacteroides ovatus strain ATCC 8483 chromosome, complete genome.
ACCESSION   NZ_CP012938 REGION: 402704..416580
VERSION     NZ_CP012938.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03852683
            Assembly: GCF_001314995.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides ovatus
  ORGANISM  Bacteroides ovatus
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Bacteroidaceae; Bacteroides.
REFERENCE   1  (bases 1 to 13877)
  AUTHORS   Wu,M., McNulty,N.P., Rodionov,D.A., Khoroshkin,M.S., Griffin,N.W.,
            Cheng,J., Latreille,P., Kerstetter,R.A., Terrapon,N., Henrissat,B.,
            Osterman,A.L. and Gordon,J.I.
  TITLE     Genetic determinants of in vivo fitness and diet responsiveness in
            multiple human gut Bacteroides
  JOURNAL   Science 350 (6256), aac5992 (2015)
   PUBMED   26430127
REFERENCE   2  (bases 1 to 13877)
  AUTHORS   Wu,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2015) Center for Genome Sciences and Systems
            Biology, Washington University School of Medicine, 4444 Forest Park
            Ave., Saint Louis, MO 63108, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP012938.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: HGAP 2.0 and Celera v. AUG-2014
            Genome Coverage       :: 350X
            Sequencing Technology :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001314995.1-RS_2025_01_08
            Annotation Date                   :: 01/08/2025 18:05:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,927
            CDSs (total)                      :: 4,834
            Genes (coding)                    :: 4,763
            CDSs (with protein)               :: 4,763
            Genes (RNA)                       :: 93
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 14 of 71
            Pseudo Genes (incomplete)         :: 53 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13877
                     /organism="Bacteroides ovatus"
                     /mol_type="genomic DNA"
                     /strain="ATCC 8483"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:8483"
                     /type_material="type strain of Bacteroides ovatus"
                     /db_xref="taxon:28116"
                     /geo_loc_name="USA:Woods Hole"
                     /collection_date="2009-10-11"
     gene            1..1635
                     /locus_tag="Bovatus_RS01515"
                     /old_locus_tag="Bovatus_00310"
                     /db_xref="GeneID:29454568"
     CDS             1..1635
                     /locus_tag="Bovatus_RS01515"
                     /old_locus_tag="Bovatus_00310"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="WP_004297908.1"
                     /db_xref="GeneID:29454568"
                     /translation="MRRIFYLLFLVLLGYSFDVKASDTVFIHETQIPVLIERQDNVLF
                     YIRLDAKESKILDEVVLDFSKSTNLADVQAIKLYYGGTEALQDQNKNRFAPVEYISSH
                     RPGATLAANPSYSIKCAEVGPSEKVVLRGNYNLFPGVNFFWISLQIKTDASLHTKIVS
                     DLRAVKVDGKELYCKFISPKAITHRMAVGVRHAGNDGSASFRIPGLVTTNKGTLLGVY
                     DVRYNSSVDLQEYVDVGLSRSTDGGKSWEKMRLPLSFGEYGGLPKAQNGVGDPSILVD
                     TQTNTVWVVAAWTHGMGNQRAWWSSHPGMDINHTAQLVLAKSTDDGKTWSKPINITEQ
                     VKDPSWYFLLQGPGRGITMSDGTLVFPTQFIDSTRIPNAGIMYSKDRGKTWKMHHLAR
                     TNTTEAQVAEIEPGVLMLNMRDNRGGSRAIAITKDLGKTWTEHPSSRKALQEPVCMAS
                     LIHVDAKDNVLNKDLLLFSNPDTTKGRNHITIKASLDKGLTWLPEHQIMLDEAEGWGY
                     SCLTMIDKETIGILYESSVAHMTFQAVKLTDLLGMK"
     gene            1789..4383
                     /locus_tag="Bovatus_RS01520"
                     /old_locus_tag="Bovatus_00311"
                     /db_xref="GeneID:29454565"
     CDS             1789..4383
                     /locus_tag="Bovatus_RS01520"
                     /old_locus_tag="Bovatus_00311"
                     /EC_number="3.2.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308806.1"
                     /GO_function="GO:0004567 - beta-mannosidase activity
                     [Evidence IEA]; GO:0005537 - D-mannose binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="WP_052587916.1"
                     /db_xref="GeneID:29454565"
                     /translation="MMINLIGKKAQIACELLCCCSMAYAQSNDNSEVVVLNTGWEFSQ
                     AGTELWRPAQVPGTVHQDLINHKQLPDPFYGINEQKIQWVENEDWEYRTAFTVTPEQL
                     KRDDAQLVFEGLDTYADVYLNGALLLKADNMFVGYTIPVKSQLRIGENLLHIYFHSPI
                     RQTLPQYNSNGFNYPADNDHHDKHLSIFSRKAPYSYGWDWGIRMVTSGIWRPVTIRFY
                     DAASISDYHVKQLALTDQLANLSNELEINNILSRPLQAEVRINTSFEGSAEKSISQAI
                     TLQPGINHVSIPSEVASPVRWMPNGWGKPALYDFSAQIIVEDKVVAEQSHRIGLRTVR
                     LVNEKDKDGESFYFEVNGVPMFAKGANYIPQDALLTNVTTERYQTLFRDIREANMNVI
                     RVWGGGTYEDDRFYDLADENGILVWQDFMFACTPYPFDPTFLKRVEAEACYNIRRLRN
                     HASLAMWCGNNEILEALKYWGFDKNFPPEIYQEMFRGYDKLFHQLLPAKVKELDADRF
                     YIHSSPYFANWGRPESWGIGDSHNWGVWYGQKTFESLDTDLPRFMSEFGFQSFPEMKT
                     ISTFAAPEDYQIESEVMNAHQKSSIGNALIRTYMERDYIIPEKFEDFVYVGLVLQGQG
                     MRHGLEAHRRNRPYCMGTLYWQLNDSWPVVSWSSIDYYGNWKALHYQAKRVFAPILIN
                     PIRQNDSLNIYLISDCPDTKDHLMLEMKVTDFDGKKQGKPIQLNTLTVPANTSQCVYR
                     IKPDTWLSPEEQQRCFMQLTLKDKAGNTLAETVYFFRKTKDLLLPETTVSCKMKQKDG
                     MCELTLFSPALAKDVFIEIPLQGARFSDNFFDLLPGERKTVVITSPQIKKGEELPLTI
                     KHIRETYN"
     gene            4418..6742
                     /locus_tag="Bovatus_RS01525"
                     /old_locus_tag="Bovatus_00312"
                     /db_xref="GeneID:29454563"
     CDS             4418..6742
                     /locus_tag="Bovatus_RS01525"
                     /old_locus_tag="Bovatus_00312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777494.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="WP_004297902.1"
                     /db_xref="GeneID:29454563"
                     /translation="MKQLFKLTGCLALAGLFASCQSVQQEANYQIIPMPQEIVTAQGS
                     PFILKSSVKILYPEGNEKMQRNAKFLADYLKTATGKDFAIEAGTEGKNAIVLALGTEN
                     ENPESYQMKVTGDGITITGPTEAGVFYGIQSLRKSLPVAVGADIAMPAVEINDAPRFG
                     YRGAHFDTSRHFFTVDEIKTYIDMQALHNMNRLHWHITDDQGWRLEIKKYPKLTEIGA
                     NRTETVIGRNSGEYDGKPYGGFYTQEQAKEIVDYAAERYITVVPEIDLPGHMQAALAA
                     YPELGCTGGPYEVWRQWGVSEDVLCAGNDQVLKFLEDVYGELIEIFPSQYIHVGGDEC
                     PKVRWEKCPKCQARIKALGLKSDQSHSKEERLQSFVINHIEKFLNDHGRQIIGWDEIL
                     EGGLAPNATVMSWRGEKGGIEAAKQKHDVIMTPNTYLYFDYYQAKDIENEPFGIGGYL
                     PMERVYSYEPMSASLTPEEQKYIKGVQANLWTEYIATFPHAQYMVLPRWAALCEIQWS
                     SPEKKNYANFLSRLPQLIKWYDAEGYNYAKHVFDVQAEFDPNPAEGTMDVTLSTIDGA
                     PVYYTLDGTEPTAASPVYEGVLKIKENVTLSAKAIRPNGESKTVTEKIDFSKSSMKPI
                     VANQPINEQYLFKGASTLIDGLKGNSSYKSGRWIAFNGNDMDMTIDLQQPTEISSVAI
                     STNVAKGDWVFDARNLSVETSDDGKTFKKIASEEYPEMKETDKDGIVEHKLTFAPVTT
                     QYVRVIASPEKSLPAWHGGKGKNAFLFVDEIKID"
     gene            6742..8817
                     /locus_tag="Bovatus_RS01530"
                     /old_locus_tag="Bovatus_00313"
                     /db_xref="GeneID:29454561"
     CDS             6742..8817
                     /locus_tag="Bovatus_RS01530"
                     /old_locus_tag="Bovatus_00313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651708.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="WP_004297901.1"
                     /db_xref="GeneID:29454561"
                     /translation="MNIRKEYPKVCLFLWILGMCFHAHPILAQSVIPVPLKMEQGTGS
                     FLLSEKTKLYTNLQGGEAELWENYLKALPVQLKEARMKDRKQMLFLLITPKTPQLPSP
                     ESYTLSVTSQRIEIRATSGAGLFYGMQTLLQLMQPASTGSYSVPSVEIEDTPRFAYRG
                     LMLDVSRHFSTKEFIKKQIDALAYYKINRLHLHLTDAAGWRLEIKKYPLLTDFAAWRT
                     DPTWKKWWNGGRKYLRYDEPGASGGYYTQDDIREILEYARQHYITVIPEIEMPSHSEE
                     VLAAYPQLSCSGEPYKNSDFCVGNEETFTFLENVLTEVMELFPSEYIHVGGDEAGKSA
                     WKTCPKCQKRMKDEHLANVDELQSYLIHRIEKFLNNHGRRLLGWDEILQGGIAPNATV
                     MSWRGEEGGIAAVTSGHHAIMTPGAYCYLDSYQDAPYSQPEAIGGYLPLKKVYAYDPV
                     PASLTAEQAKLVYGVQGNLWVEYIPTPEHVEYMIYPRMLALAEVAWSAPERKSWPDFH
                     TRALSAVADLQKKGYHPFDLSKEIGSRPESLQPVSHLALGKKVTYNSSYSPHYPAQGN
                     TALTDGIRGDWTYGDGSWQGFISDNRLDVTIDMEKETPIHSITAAFMQVVGAEVFLPE
                     TVIISISDDGINFTELQKQHFEVSKETPIRFTDISWQGEAKGRYVRYQAQAGSEFGGW
                     IFTDEIIVK"
     gene            8916..11660
                     /locus_tag="Bovatus_RS01535"
                     /old_locus_tag="Bovatus_00314"
                     /db_xref="GeneID:29454556"
     CDS             8916..11660
                     /locus_tag="Bovatus_RS01535"
                     /old_locus_tag="Bovatus_00314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644161.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="WP_004297899.1"
                     /db_xref="GeneID:29454556"
                     /translation="MVQHARLIFYSLLLLVIPCESTLAQKIPVTPIDSLITVGYATGS
                     LKTLSGSVEKITETQMNKDQITNPLEAIRGRVPGLTIQRGSNGPAALDAVRLRGTTSL
                     TSGNDPLIIVDGVFGDLSMLTSIYPTDIESFTILKDASETAQYGSRGASGVIEVTTKK
                     GMSGRTQVAYNGSFGISTVYKNLKMLSGDEYRRIASERGISILDKGYNTDFQKEIEQT
                     GLQQNHHIAFYGGSSESSYRVSLGFMDRQGVILNEDMKNFTSNMNMNQKMFDGFLNCE
                     LGMFGSIQKNHNLVDYQKTFYSAATFNPTYPNHKDPVTNSWDGITTASQITNPLAWME
                     VQDDDATSHISTHARLTFNLLEGLKLNLFGAYTYNIVENSQYLPTSVWANGQAYKGTK
                     KRESLLGNMMLTYKKNWKKHFFDVLALAELQKETYTGYYTTVSNFSTDKFGYNNLQAG
                     ALRLWEGTNSYYDQPRLASFMGRFNYTYADRYVLTLNARTDASSKFGANHKWGFFPSA
                     SAAWVISEEEFMKQLPMVDNLKFRIGYGLAGNQSGIDSYTTLNLVKPNGVVPVGNSAV
                     VSLGDLRNTNPDLKWEVKHTFNTGIDVALFGNRLLLSANYYNSRTTDMLYLYNVSVPP
                     FTYNTLLANIGSMRNWGTEIAIGITPLKTKDMELNINANITFQRNKLLSLSGMYNGEM
                     LSAPEYKSLSGLDGAGFHGGYNHIVYQMVGQPLGVFYLPHSTGLESDGNGGYTYGIAD
                     LNGGGVSLEDGEDRYVAGQAVPKTILGSNISFRYKRFDLSLQINGAFGHKIYNGTSLT
                     YMNMNIFPDYNVMKKAPKQNIKDQTATDYWLEKGDYVNFDYVTLGWNVPIEKVQKLKK
                     YVRSLRLAFTVNNLATISGYSGLSPMINSSTVNSTLGVDDKRGYPLARTYTLGLSINF
                     "
     gene            11672..13390
                     /locus_tag="Bovatus_RS01540"
                     /old_locus_tag="Bovatus_00315"
                     /db_xref="GeneID:29454555"
     CDS             11672..13390
                     /locus_tag="Bovatus_RS01540"
                     /old_locus_tag="Bovatus_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_004326849.1"
                     /db_xref="GeneID:29454555"
                     /translation="MMKQYMKNKSLFLQKEELLSRIAELFSKKILRPSGVLVFLLITC
                     LGLFSCSKFLEENPKDKLPEDDVYNTISEVYLNAVASLYTYVGGYSDSQGLQGTGRGV
                     YDLNTFTSDEAIIPTRGGDWYDGGFWQGLYLHDWGIENDAIQATWEYLYKVVMLSNKS
                     LERIDKFAETHSATELPAYRAEVRAMRAMYYYYLMDLFGRIPLVQSSSVAMKDVVQSE
                     RKTVFDFVVKELQEAAPLLSDAHSNQSGPYYGRITRPVVTFLLAKLALNSEVYTDNDW
                     TDGQRPDGKNIKFTVNGSELNAWETVIYYCDQLKTMGYKLEPEYETNFSIFNEPSVEN
                     VFTIPMNKTLYTNQMQYLFRSRHYNHAKAYGLSGENGPSATIEALETFGYETAEQDPR
                     FDICYFAGIVHDLKGNIIKLDNGTVLEYLPWKVSLDITDTPYEQTAGARMKKYEVDPT
                     ATKDGKLMENDIVLFRYADALLMKSEAKVRNGANGDEELNEVRSRVNASPRTATLENI
                     LAERQLELAWEGWRRQDLVRFGKFTRAYSSRPQLPDEASGYTTVFPIPEKIRVMNERL
                     KQNPGY"
     gene            13485..13877
                     /locus_tag="Bovatus_RS01545"
                     /old_locus_tag="Bovatus_00316"
                     /db_xref="GeneID:29454554"
     CDS             13485..13877
                     /locus_tag="Bovatus_RS01545"
                     /old_locus_tag="Bovatus_00316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832518.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_004297896.1"
                     /db_xref="GeneID:29454554"
                     /translation="MKTVEVIVEHAGKNLSAYIEDAPVITVGNDMREIEDNMREAIEL
                     YLEDNSNPCEVLSGEFELKFKIDAATFINYYSNIFTKAALSRITGINERQLWHYAAGV
                     HKPRRQQLEKIQKGIQALSKELSAINLL"
ORIGIN      
        1 atgagaagaa ttttttattt acttttcctt gtcttattag gctattcttt tgacgtaaaa
       61 gcttcagaca cagtatttat ccatgaaaca caaatcccgg ttttgataga gcggcaggat
      121 aatgttctgt tctatatccg gttggatgca aaggaaagca agatactcga tgaagttgtt
      181 ttagatttca gtaaaagtac caacttagcg gatgttcagg caatcaaact ctattatgga
      241 ggaacagagg ccttacaaga tcaaaacaaa aatcgctttg cccctgtaga gtacatatcc
      301 agccatcgac cgggtgcgac attggcggct aatccttcct attccataaa atgtgcagag
      361 gtcggaccat cggaaaaagt tgtattaaga ggtaattaca atttatttcc aggtgtcaac
      421 tttttttgga tcagtttgca gataaaaaca gatgcctctt tgcatactaa aatagtctct
      481 gatttacgtg ctgtaaaagt ggatgggaaa gaactttatt gtaaatttat ttctccgaaa
      541 gctattactc accggatggc agtcggagtt cgtcatgctg gtaatgacgg ttctgcttcc
      601 ttccgtattc ccggattagt gacaaccaat aaaggaacat tattgggagt gtacgatgtc
      661 cgatataata gtagtgtcga tttacaggaa tatgtagatg tgggtttgag tcgtagcact
      721 gacggaggga agagctggga gaaaatgcgt cttccgcttt catttggtga gtatggcggt
      781 ttgcctaaag cacagaatgg ggtaggagat ccctctattt tggtagatac tcaaacgaat
      841 accgtctggg tggttgccgc atggacacat ggtatgggta atcaacgtgc ctggtggagc
      901 tcccatccgg gaatggatat aaaccatacc gcacagctgg tattggcaaa gagcacagac
      961 gatggtaaaa catggtcgaa accgatcaat ataacagagc aggtaaaaga tccgtcctgg
     1021 tattttctcc ttcaaggacc aggacgagga ataacgatga gcgacggtac attagtattt
     1081 ccaacacagt ttatcgactc tacccgtatc ccgaatgcag gaatcatgta tagtaaagac
     1141 cggggaaaaa catggaaaat gcatcatctg gcacgaacaa atacaacgga agcacaagtt
     1201 gcggaaatag aaccgggagt attgatgctg aatatgcgtg acaatagagg aggaagccgg
     1261 gctattgcca taacgaaaga tttgggaaaa acctggacag aacacccatc ttcacgtaag
     1321 gcacttcagg aaccggtatg tatggccagt ctgattcatg tcgatgccaa ggacaacgtt
     1381 ttgaataaag acctcctgtt gttttcaaat ccggatacga ctaaagggcg caatcatata
     1441 acaataaaag caagtctgga taaaggactt acctggttgc ccgaacatca aatcatgctc
     1501 gacgaagcag aaggatgggg atatagttgc ctgacgatga ttgataaaga gacaatcggt
     1561 attctatatg agagcagtgt ggcacacatg acttttcagg cggtgaaatt aacagatttg
     1621 ttaggaatga aatgatagat ataatagcgg ttaaactatc tgatatgata aaagaataaa
     1681 accgtatatt tatcgtggcg tgcaaaaaca acatattttg ttaaattatc agtgaaattt
     1741 gctgaaaaaa taatatcttt gaatccgtta atatctaatg cccccactat gatgattaat
     1801 ttgatcggaa aaaaggctca aatagcatgt gaactactat gctgttgcag tatggcatat
     1861 gcacagagca atgacaattc cgaagttgtc gttctaaata caggctggga gttttcccag
     1921 gcagggacgg agctgtggcg accggcacag gttccgggca ccgttcatca ggatcttatt
     1981 aaccataaac aacttcccga tcctttttat ggcatcaatg aacaaaagat tcagtgggtg
     2041 gaaaatgaag actgggaata ccggactgct tttacagtta cccccgaaca actgaaacgg
     2101 gatgacgccc aattggtttt tgaaggcctt gacacatacg ccgatgtata cctcaatgga
     2161 gcactgttgc tgaaagccga taacatgttt gtcggttata ccatacccgt caagtcccaa
     2221 ctccgtatcg gagaaaacct tcttcacatc tattttcatt cccccatccg gcagacactg
     2281 cctcaatata actcgaacgg attcaactat ccggcagaca acgaccatca cgacaaacat
     2341 ttaagcatat tctcccgtaa agccccttac agctatggct gggactgggg aatccgcatg
     2401 gtgaccagcg gcatctggcg tccggtgacc atccgttttt atgacgcagc ttctatcagt
     2461 gactaccacg taaaacaact cgcactgacc gaccagctcg caaacctgtc taatgaatta
     2521 gaaataaaca acatactctc ccggccactt caggcagagg tgagaatcaa tacctcattc
     2581 gaaggaagtg cagaaaaaag catcagccag gcaatcacct tacagcccgg aatcaatcat
     2641 gtttccatac cttccgaagt ggcatccccc gtacgttgga tgccgaatgg atggggaaag
     2701 cccgccctgt atgatttctc cgctcaaatc atagtagaag ataaagtcgt agccgagcaa
     2761 tcgcaccgga taggactgcg tactgttcgc ctcgttaatg aaaaagataa agacggagaa
     2821 tccttctatt ttgaagtaaa tggtgtacct atgtttgcca aaggagccaa ttacatcccc
     2881 caggatgcac tcttgactaa cgtcactacc gaacgctacc aaaccttatt ccgcgacatc
     2941 cgcgaagcga acatgaatgt cattcgtgta tggggaggtg gaacgtacga agatgaccgt
     3001 ttctatgacc tggcagatga aaacggaata ctggtgtggc aagacttcat gtttgcctgc
     3061 actccctatc cgttcgaccc cacctttctg aaaagggtag aggcggaagc ctgctataac
     3121 atccgtcgcc ttcgcaatca tgcttccctg gcgatgtggt gcggcaataa cgaaatactg
     3181 gaagccttaa aatattgggg attcgacaag aacttcccgc ccgaaatcta tcaggaaatg
     3241 tttcgaggct acgacaaact gttccatcag ctccttccag ccaaggttaa ggaactggat
     3301 gccgaccgtt tctacataca cagctccccg tattttgcca actgggggcg tcctgaatcc
     3361 tgggggatag gagacagcca taactgggga gtctggtatg gtcagaaaac attcgagtca
     3421 ctcgatacag acctgccccg tttcatgagc gaattcggtt ttcagtcctt cccggaaatg
     3481 aagaccatct ccactttcgc cgctccggaa gattatcaga tagaatcgga agtgatgaat
     3541 gctcatcaga aaagcagcat cggcaacgcc ctgattcgta cctacatgga gcgcgactat
     3601 atcatccccg aaaaatttga agactttgtc tatgtcggac tagtactgca aggacaagga
     3661 atgcgtcacg gactggaagc acaccgccgg aaccgtcctt actgtatggg taccctgtat
     3721 tggcaactga atgacagctg gccggtagta tcctggtcga gcatcgacta ttacggtaac
     3781 tggaaagccc tgcattacca agccaaacga gtctttgctc caatcctcat caatcctatc
     3841 cggcagaacg acagcctcaa tatctacctg atttccgact gtccggacac caaagaccac
     3901 ctgatgttgg aaatgaaagt aacagacttt gacggaaaga aacaaggcaa gcctatacaa
     3961 ctcaacacat tgacagttcc ggctaacact tcccaatgcg tgtaccgtat aaaacccgac
     4021 acctggctat caccggaaga acagcaacgc tgtttcatgc aactgacgct aaaagacaag
     4081 gcaggaaaca cccttgccga aaccgtatac ttctttagaa aaacaaaaga cctgcttctg
     4141 cctgaaacaa ccgtttcctg caagatgaaa cagaaagacg gcatgtgtga actgacattg
     4201 ttttctcccg ctcttgcaaa agatgtcttt attgaaattc ctttgcaggg agcccgtttc
     4261 agcgacaact ttttcgacct tcttcccgga gaacgtaaaa cggtcgtcat cacttctccc
     4321 caaataaaga aaggagagga gctgcctttg acaatcaaac atattcgtga aacttataat
     4381 taatctatta ataacttaac acttaatttt ttagtttatg aagcaactct ttaaattaac
     4441 cggatgtctg gcactagcag gactttttgc ctcttgccaa tcggtgcaac aggaagccaa
     4501 ctaccagatc atccccatgc cacaagaaat tgtaaccgct caaggaagtc ccttcatcct
     4561 caagagcagt gtgaaaattc tctatccgga aggaaacgag aagatgcaac gcaacgcaaa
     4621 attcctggca gactatctga aaacggctac cggaaaagat ttcgccattg aagccggaac
     4681 agaaggtaaa aacgcaatcg tactggcatt aggcacggag aacgaaaatc cggaatccta
     4741 ccagatgaaa gttaccggtg acggaatcac gattactggt cctacggaag ccggcgtatt
     4801 ctatggcatc cagtccctgc gcaaatcatt gcctgtggct gtcggtgcgg atattgccat
     4861 gccagccgta gaaatcaatg acgctccccg ctttggctat cgtggcgcgc atttcgatac
     4921 cagccgtcat ttctttaccg tggatgaaat aaagacctac atcgacatgc aggctttaca
     4981 taacatgaac cgcctccact ggcatatcac cgacgatcaa ggttggcgtc tggaaatcaa
     5041 aaaatacccg aaactgactg aaatcggtgc taacagaaca gaaacagtca tcggacgtaa
     5101 ctccggcgaa tacgacggca aaccttacgg tggattctac acccaggaac aagccaaaga
     5161 aattgtagat tatgcagccg aacgctacat caccgttgtt cccgaaatcg accttccggg
     5221 acacatgcaa gctgcccttg ccgcttaccc tgaactgggt tgtaccggtg gtccatacga
     5281 agtatggaga caatggggtg tatccgagga tgtgctttgc gccggaaacg accaagtatt
     5341 gaaattcttg gaagatgttt atggtgaatt gattgaaatc ttcccatcac aatatattca
     5401 tgtagggggc gacgaatgcc cgaaagtaag atgggaaaaa tgtccgaagt gccaggcacg
     5461 tatcaaagca ttgggattga agtcagatca gagccacagc aaagaagaac gtttgcagag
     5521 ctttgtcatc aaccatattg agaaattcct gaacgaccac ggacgtcaaa tcatcggttg
     5581 ggatgaaatc ctcgaaggcg gacttgctcc gaatgctacc gtgatgtcat ggcgtggtga
     5641 gaaaggtggt atcgaagctg cgaagcagaa acacgatgtc atcatgacac cgaacaccta
     5701 tctgtatttc gactactacc aagctaaaga catcgaaaac gaaccgttcg gtatcggtgg
     5761 ttatctgccg atggaaagag tgtatagcta cgagccgatg tcggcttctc ttactccgga
     5821 agaacagaaa tacatcaagg gagtgcaagc caacctctgg acagaataca tcgctacctt
     5881 cccgcatgca caatacatgg tattgcctcg ttgggctgct ttgtgtgaaa ttcagtggtc
     5941 cagccccgaa aagaaaaact atgccaactt cctgtcccgt cttccacaac tgattaagtg
     6001 gtacgatgca gaaggataca attacgctaa acacgtattt gacgtacaag ccgagtttga
     6061 cccgaatccg gcagaaggaa caatggacgt taccctgtct accatcgacg gtgcccctgt
     6121 ttactataca ttagacggca cagaacctac tgccgcttcc cccgtttacg aaggagtatt
     6181 gaaaatcaag gaaaatgtaa ccctgtccgc caaagccatt cgtcccaacg gcgaaagcaa
     6241 gacagtgaca gagaagattg atttcagcaa atccagcatg aagccgattg tagccaatca
     6301 gcctatcaat gaacaatacc tgttcaaagg agcttccact ttgatagatg gtctgaaagg
     6361 aaatagcagc tataagtcag gccgttggat cgctttcaac ggaaacgaca tggatatgac
     6421 tatcgacctc cagcaaccta ctgaaatctc aagcgtggca atctctacca atgtagcaaa
     6481 aggggactgg gtatttgatg ccagaaacct gtccgtagaa acttcagatg atggcaagac
     6541 cttcaaaaag attgcttccg aagaatatcc ggagatgaaa gagacagaca aagacggaat
     6601 cgttgagcac aaactaacct ttgctcctgt gaccacccaa tacgtgagag tcattgcttc
     6661 accggagaaa tcacttccgg catggcatgg aggaaaaggt aaaaacgcat ttttatttgt
     6721 agacgaaatc aaaattgatt aatgaatata agaaaagaat accctaaagt ttgtcttttc
     6781 ttatggatat tggggatgtg cttccatgca catcccattt tggcacagtc cgtcatacct
     6841 gtcccattaa aaatggagca ggggacgggc tcttttttac tgtcagaaaa gactaaactt
     6901 tatacaaacc tgcaaggtgg agaagcggaa ctctgggaga attacctgaa agcattacct
     6961 gttcagctga aagaagcgag aatgaaagac aggaaacaga tgcttttctt attgataacc
     7021 cccaagaccc cccaattgcc atcaccggaa agctatacac tctccgtcac ctctcaacga
     7081 attgagatcc gggcaacttc gggagccgga ttattctatg gaatgcaaac cctgttacag
     7141 ctgatgcaac cggcaagcac aggcagctat tccgtaccct ctgtcgagat agaagacacc
     7201 cctcgttttg cttatcgcgg actgatgctg gacgtatccc gtcatttctc taccaaagag
     7261 tttataaaga agcagataga cgcattggca tattacaaaa tcaaccgttt gcacctgcac
     7321 ctgaccgatg cggcaggctg gcggcttgaa atcaagaaat accctttact gaccgatttt
     7381 gcagcctggc gtacagaccc cacctggaag aaatggtgga acggcggacg taaatacctc
     7441 cgttatgatg aacccggagc ttccggcggc tattacaccc aggacgatat tcgtgaaata
     7501 ctggaatatg cccgtcagca ttacatcacg gtgattccgg agattgaaat gccttcccat
     7561 tcggaagaag tgctggctgc ctacccgcag ctatcctgtt cgggagaacc ttacaagaac
     7621 tccgatttct gtgtcggtaa tgaagaaact ttcacctttc tggagaatgt actgacggaa
     7681 gtcatggaac ttttcccttc cgaatacatt catgtaggag gtgatgaggc cggcaaatcg
     7741 gcttggaaga cctgtccgaa atgtcagaag agaatgaaag acgagcatct ggccaatgta
     7801 gacgagctgc aaagctatct gatacaccgt atcgaaaagt tcctcaacaa tcacggacgc
     7861 cgtctcttgg gatgggacga gattctgcaa ggaggtatcg cgcccaatgc aaccgttatg
     7921 tcgtggcgtg gagaagaggg tggtattgct gccgtcacct ccggccatca tgccatcatg
     7981 acaccgggtg catactgtta tctggacagc tatcaggacg ccccgtactc ccagccggaa
     8041 gctatcggtg gctacctgcc attgaagaaa gtatatgcct acgatcctgt ccccgcttcc
     8101 ttaactgcgg agcaggcaaa gttagtctat ggtgtgcagg gtaacctatg ggtggaatat
     8161 atccccactc ccgaacacgt ggaatatatg atttatcccc gtatgctggc attggcggaa
     8221 gtagcctggt cggctccgga acgtaagtcg tggcccgatt tccatacccg cgcactgtca
     8281 gcagtggccg atttacagaa aaaaggatac catcctttcg atttgagcaa agaaatcggc
     8341 agccgtcccg aatctcttca accagtcagc catttggcgc tcggtaaaaa agtgacctat
     8401 aactcctcct atagccccca ttatcccgca caaggcaaca cagccctgac agacggcata
     8461 cgtggtgact ggacttacgg tgatggttca tggcaaggat ttatctcgga caaccgtctc
     8521 gatgtgacga ttgatatgga aaaagaaacc cctatccatt ccatcaccgc cgcttttatg
     8581 caagtggtcg gcgcggaagt attcctgccg gagacagtga ttatttccat ttccgatgat
     8641 ggaattaact tcacggagtt gcaaaaacaa cactttgaag tgagcaaaga aacccctatt
     8701 cggtttacgg atatttcatg gcagggagaa gccaaaggaa gatacgtacg ttaccaggca
     8761 caggccggaa gcgaattcgg aggttggata tttacggatg agataatcgt gaaatagaca
     8821 ttgtcaatat catttatgaa taaaagaggg aatctactca accagattcc ctcttttttt
     8881 gttatatttg tattattacc ttaaaaacta agaatatggt acaacacgct agactcattt
     8941 tctacagcct tcttcttctg gtaatcccgt gcgagagcac cttggcgcag aagattcccg
     9001 tcacgcccat agactcattg ataactgttg gatatgccac cggaagtttg aaaaccctct
     9061 ccggttccgt agagaagatt acggagacac agatgaataa agaccagata acgaaccctt
     9121 tggaagcaat ccgcggacgt gtgccgggct tgaccatcca acgcggatca aacggtccgg
     9181 cagccttgga tgcggtacgc ctgcggggaa ctacctcact gaccagtggc aatgaccctt
     9241 tgataatcgt cgacggagta tttggcgact tgagcatgct cacctccatt taccctacgg
     9301 atattgagag cttcaccatt ttgaaagacg cttccgaaac cgcacaatac ggttcacgag
     9361 gagcttccgg tgtgatcgaa gtcaccacta aaaaaggaat gagcggcaga acacaagtag
     9421 cctacaacgg tagcttcgga atttccaccg tctacaagaa tctgaaaatg ctgtccggcg
     9481 atgagtaccg ccgtatcgct tcggaacgcg ggatttccat tctggacaaa ggatataata
     9541 ccgattttca gaaagagatt gaacagacag gactacaaca aaaccatcac atcgcctttt
     9601 atggtggttc cagtgagtcc agctatcgcg tttctcttgg tttcatggat cgtcagggag
     9661 tgattttgaa tgaagacatg aaaaacttca cttcgaatat gaacatgaat cagaaaatgt
     9721 tcgatggctt cctgaactgc gagctgggaa tgttcggttc cattcagaag aatcataatc
     9781 tggtggatta tcagaaaacg ttctattcgg cagccacatt caatcccacc tatcccaacc
     9841 ataaagaccc cgtcaccaat tcctgggacg gaataacaac cgccagccaa ataaccaatc
     9901 cattggcatg gatggaagtg caggatgatg atgccacttc gcacatcagc acgcacgccc
     9961 gtctgacttt caatctgctg gaagggctga aacttaacct cttcggtgcc tatacctata
    10021 atatcgtgga aaattcgcaa taccttccca cttccgtctg ggcgaacggc caggcttaca
    10081 aaggaaccaa aaagcgagaa tccttgctgg gcaacatgat gctgacctat aagaaaaact
    10141 ggaaaaaaca tttctttgac gtgctggcac ttgccgaact tcaaaaagaa acctacacgg
    10201 gatattatac aacagtaagc aatttcagca ccgacaaatt cgggtataat aatcttcagg
    10261 caggcgcact ccgcttgtgg gaaggcacta attcttacta cgaccaaccc cgtctggcat
    10321 ctttcatggg acgtttcaac tatacatacg ccgaccgcta cgtactgacg ctgaacgccc
    10381 gtacagatgc ttcctccaaa ttcggagcga accataaatg gggattcttc ccctccgcat
    10441 ctgccgcatg ggtgattagt gaagaagaat ttatgaagca gttgccgatg gtggataatc
    10501 tgaaattccg tattggctac ggtctggcag gtaatcagag tgggattgac tcatacacta
    10561 cattgaacct tgtaaagccg aacggtgtag tccccgtcgg aaactccgcc gtcgtatctt
    10621 taggagactt gcggaacacg aatcccgacc tgaaatggga agtgaaacat acctttaata
    10681 ccggtatcga cgtagctctt ttcggcaacc gcctgctgct ttccgccaac tattacaact
    10741 cccgaaccac agacatgctt tacctttaca acgtaagcgt accgcctttc acctataaca
    10801 ccttgcttgc caacatcggt tccatgcgca actggggtac ggaaatcgcc atcggcatca
    10861 cccccttgaa gaccaaggat atggaactga atatcaatgc caatatcact ttccagcgca
    10921 acaaactgct ctcattaagc ggtatgtaca acggcgaaat gctttctgct cccgaatata
    10981 aaagtctttc tggcctcgac ggagccggct ttcatggagg atacaaccac atcgtatatc
    11041 agatggtggg tcaaccgttg ggcgtgttct atcttcccca cagtacggga ctggaatccg
    11101 acggaaacgg cggatacacc tatggcatcg ccgatctgaa tggcggaggt gtcagccttg
    11161 aagatggtga agaccgctac gtggcaggac aagccgtacc taaaacgatt cttggctcca
    11221 acatcagttt ccgctacaag cgtttcgatc tgtctttgca gattaacgga gctttcggac
    11281 ataaaatcta caacggaacc tccctgacct atatgaacat gaatatattt cccgactata
    11341 acgtaatgaa gaaagccccg aaacagaaca tcaaggatca aaccgccacc gactattggt
    11401 tggaaaaggg agattatgtc aacttcgact atgtgacgct gggttggaac gtgccgattg
    11461 agaaagtaca gaaactgaag aaatatgtcc gctccctgcg tctggcattt acagtcaaca
    11521 acctggcaac catttccggt tattcgggac tttcacccat gattaacagt tcaacggtga
    11581 actccacttt aggcgtggat gataaacggg gatatccgtt ggcgcggacg tacacactgg
    11641 gattgagtat taacttttaa tggaggagga gatgatgaaa caatatatga aaaataaaag
    11701 tctttttttg cagaaagaag aactgctttc aaggatagcg gaactgtttt caaagaagat
    11761 cttacgtcct tcgggagtgc tggtattcct tcttataacg tgcctgggtt tattctcatg
    11821 tagtaagttt ttggaggaaa atccgaaaga taagttaccc gaagatgacg tttacaatac
    11881 aatctcggaa gtatatctca atgcagtagc ttcgctttat acatacgtag gcggttatag
    11941 tgatagtcag ggattgcagg gaacgggtag gggagtctac gacctgaaca cctttacctc
    12001 cgacgaagcc attatcccca cccgtggagg tgactggtat gacggtggtt tttggcaagg
    12061 actttacctg cacgattggg gaatagagaa tgatgccatt caggctacat gggaatatct
    12121 ctataaagtc gtcatgttaa gtaacaaatc actggaacgg atagacaaat tcgccgaaac
    12181 tcattccgct acggaactgc cggcataccg tgccgaagta cgtgccatgc gggctatgta
    12241 ttattattac ctgatggatt tgttcggacg catcccgcta gttcaatcct cttccgtagc
    12301 aatgaaagat gtagtgcaaa gtgaacgaaa aacggtcttt gactttgttg tcaaggaatt
    12361 gcaagaggcg gctcctttgt tgagcgatgc acatagcaac cagtccggcc cttactatgg
    12421 ccgtatcact cgccccgtag taactttcct actggccaaa ctggctttga actccgaagt
    12481 ttatacagac aatgattgga cggacggaca acgcccggat ggaaaaaata taaagttcac
    12541 agtgaacggc agcgaactaa atgcctggga aacagtcatc tactattgtg accaactcaa
    12601 gactatgggc tacaaactgg aaccggaata cgaaacgaat ttctctatat tcaacgaacc
    12661 gtccgtagag aatgtcttca ctatcccgat gaataaaact ctgtacacca accagatgca
    12721 atatctcttc cgttcccgcc actataatca cgccaaggct tacggactaa gcggtgagaa
    12781 cggtcccagc gccaccattg aagccctcga aactttcggt tatgaaaccg cggaacaaga
    12841 tccccgtttt gatatttgct attttgcagg aattgtccac gacctgaaag gaaacataat
    12901 caaactggat aatggaacgg tactggaata tctgccctgg aaagtatctc tggatataac
    12961 agatacccca tacgaacaaa ccgcaggagc ccgcatgaaa aagtatgaag ttgatccgac
    13021 tgccacaaaa gacggtaagt tgatggagaa tgacattgta ttgttccgct atgcagacgc
    13081 tttgttgatg aagagtgaag cgaaagtccg gaacggagca aacggagatg aagaactcaa
    13141 tgaagttcgt agccgtgtca acgcttctcc ccgcactgcc actttagaga atatccttgc
    13201 cgaacgtcag ctggaactgg cttgggaagg atggagacga caggacttag tccgcttcgg
    13261 gaagtttaca agagcttaca gcagccgtcc gcaactaccg gatgaggcaa gtggatatac
    13321 aactgtattc cctataccgg agaaaatacg ggtgatgaat gagaggttga aacagaatcc
    13381 ggggtattag gaagaaatca tagtgaaaat atgaatactt cttttggtag tgttcatata
    13441 attactatat ttgtgcagta attaagtccg atataggagg agatatgaaa acagtagaag
    13501 taattgtgga acacgcagga aagaacttga gtgcttatat tgaagatgct ccggttatca
    13561 ctgttggcaa tgacatgaga gagattgagg acaacatgcg agaggcgatt gaattgtatt
    13621 tggaagataa ttccaatccc tgtgaagtat tgtccggaga gtttgaatta aaattcaaga
    13681 ttgatgctgc tacatttata aactattata gcaacatctt cactaaagct gcattgagtc
    13741 ggattacggg tattaatgaa cggcaattat ggcattatgc tgccggagtg cataagcctc
    13801 gcaggcaaca acttgaaaaa atacaaaaag gtattcaggc tttatcaaag gagttatcgg
    13861 caatcaattt attgtaa
//
