LOCUS       NC_011898              18498 bp    DNA     linear   CON 07-APR-2024
DEFINITION  Ruminiclostridium cellulolyticum H10, complete sequence.
ACCESSION   NC_011898 REGION: 1548963..1567460
VERSION     NC_011898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00623037
            Assembly: GCF_000022065.1
KEYWORDS    RefSeq.
SOURCE      Ruminiclostridium cellulolyticum H10
  ORGANISM  Ruminiclostridium cellulolyticum H10
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 18498)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O.,
            Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M.,
            Hauser,L., Kyrpides,N., Ivanova,N., Zhou,J. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Clostridium cellulolyticum H10
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18498)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O.,
            Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M.,
            Hauser,L., Kyrpides,N., Ivanova,N., Zhou,J. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2009) US DOE Joint Genome Institute, 2800
            Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP001348.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4002584
            Source DNA and bacteria available from Jizhong Zhou
            (jzhou@rccc.ou.edu)
            Contacts: Jizhong Zhou (jzhou@rccc.ou.edu)
                      David Bruce (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000022065.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 08:30:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,555
            CDSs (total)                      :: 3,465
            Genes (coding)                    :: 3,413
            CDSs (with protein)               :: 3,413
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 12 of 52
            Pseudo Genes (incomplete)         :: 38 of 52
            Pseudo Genes (internal stop)      :: 9 of 52
            Pseudo Genes (multiple problems)  :: 7 of 52
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18498
                     /organism="Ruminiclostridium cellulolyticum H10"
                     /mol_type="genomic DNA"
                     /strain="H10"
                     /culture_collection="ATCC:35319"
                     /type_material="type strain of Clostridium cellulolyticum"
                     /db_xref="taxon:394503"
     gene            1..1725
                     /locus_tag="CCEL_RS06265"
                     /old_locus_tag="Ccel_1250"
     CDS             1..1725
                     /locus_tag="CCEL_RS06265"
                     /old_locus_tag="Ccel_1250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616937.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="WP_015924755.1"
                     /translation="MVKLKMRNSIFSRLVITFMIIIIPLYSLGIYIYGSSLRTVKSEI
                     SKSTVAQASFYLESLEKEIERIKILQYDCLNDEQLNKLAIRWEIMSEYEISQTLLQLK
                     QRLVAIKSSSIYVNDVNVHILPISKTVSSNSGVDDIHINEYNGIYVPAGLKGAQIINY
                     NGQLYLSTFKKYSGATKPLFTINIELNQNALKQALEQFNTYAGSGSILITPTNIIANK
                     SQEDNSQLIQSILLSMKERDRDGTVFTKIGNKQYYVVHSNSSYLNMLLLRYFPQEFIL
                     KPLENFKTWAWVFSIAAILIIIIYSFSTYKFMHQPLNELVKSFRKIENGDLKVFISHD
                     SNNEFGYLYKCFNDMVKNLNMLIDQVYNQKILMQKAELKHLQSQINPHFLYNSFFMIN
                     TMARIGDENLVPFTKHLGEYFRFVTRNSMDNLSLEEEINHAKVYTEIQLMRFSKRIQI
                     HFGECPNMYKDLKVPRLILQPIIENAFEHGLEKKKNGGLLLVNFEGNERELRIVVEDN
                     GFDITDSELEKLQNSLEDNGLEIETTGTLNIHRRIRLVFGEESGLIISRSVIGGLKAV
                     LKIVLQKE"
     gene            1730..3430
                     /locus_tag="CCEL_RS06270"
                     /old_locus_tag="Ccel_1251"
     CDS             1730..3430
                     /locus_tag="CCEL_RS06270"
                     /old_locus_tag="Ccel_1251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312574.1"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_015924756.1"
                     /translation="MYRLLIVDDEEIIVNGLYEIFRSLKNLDLDVYRAYSGEEAVEWL
                     SRTRMDIVLTDIRMPEMDGLQLLDVIYRRWPQCKVIFLTGYNEFEYVYKAIQHQGVSY
                     ILKTEDNDKVISVVENAIEEIKKEIKTEDLIHNAKEQIELALGLFQKDYFIHLLHEEN
                     SSDVNKAQFEQLAIPMYPDHPVILLLGHIDSIPGNLSYWDKIQYINSIKVIITKNLNT
                     NIRSICILDDRYRFIFFIQPNELMTTGFSKTEENAFYDKAISFVKGTLEVIQTACMES
                     LNASISFSLSGEPCNWEDVSKKYFSLNQLLNYKIGTGTEMLLIDNELKNNILASGTEV
                     PELDTNDEPLEILLRRKNMDLIQQYMESGQKEKYFEVLEELLTPIKSVKSKNSNLAIE
                     AYSMVSLSILSYINRWKITEKLAFHIGLNSLMRIDKHETWSDAVKYLINISDIIFQLQ
                     TDEQKKRADNAVDFVQSFINEHLSEDLSLVRLAEQVYLNPSYLSRLYKQVTNTNLSDF
                     IDNARIERAKELLKKEKVKINEVAKAVGYETAASFTRFFKKLMGCSPQEYHDTMLSGK
                     "
     gene            3589..5304
                     /locus_tag="CCEL_RS06275"
                     /old_locus_tag="Ccel_1252"
     CDS             3589..5304
                     /locus_tag="CCEL_RS06275"
                     /old_locus_tag="Ccel_1252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="WP_015924757.1"
                     /translation="MRFNYLKKAVSLAVVLSLTASIVVACGSQNNTSDSTASSSQSTT
                     SSVANKDPFGKYSPEIDISFVRGIDDDLAANILPKTPGETLEDNRWTKLYKDELGINV
                     KYAWTVKGNETSDAYTQKINVTLASGELPDVVLVNPSQLKQLVDSDMIEDMTQYYNDY
                     ASEDFKKLMTEEGTGNIDSAMFDGKMMAIPEPVSTNETAHYLWIRNDWLKKLNLQAPK
                     TMDDVLKISEAFTTQDPDGNGKNDTFGLPITKDIYSGCMGTEGFFAGYHAYPNMWIED
                     DSGKIVWGSTLPETKVALQKLADMYKSGQLDKEFGVKDGGKVAETIAAGKVGINYGQQ
                     WNPMYPLISNFNNDKNADWTAYPIVSNDDKKVMVPLKFNQTRIFAVKKGYEHPEALVK
                     LFNAHVEKNWGKTADFNKYYMPVENGGVGVWKFSPVCPAPVFKNLEAFVAIDEARNNN
                     DFSKLTGEPKIIQGNIEAYAKGDTSQWGWEKIYGKEGAFRNMVEYKKNDQLLKEKFVG
                     APTTTMAEKKTTLEKMEKEVFIKIIMGAAPISEFDKFVSDWNKLGGADMTKEVNEWYD
                     SVKSK"
     gene            5410..6312
                     /locus_tag="CCEL_RS06280"
                     /old_locus_tag="Ccel_1253"
     CDS             5410..6312
                     /locus_tag="CCEL_RS06280"
                     /old_locus_tag="Ccel_1253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312576.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_015924758.1"
                     /translation="MKNLNIKKQLPLHLMILPGFITLLIFSYLPMAGIVIAFQKFIPA
                     KGLFGDQKWIGFKNFEYVLNMPNFDNIMWNTISIAMAKIILGLIIPIIFAILINEVTN
                     NALKRGIQTLIYLPHFLSWVVLGGILIDILSPSGGILNSFIKSLGFEPIFFLGDNKWF
                     PSTMVLTETWKEFGYGTIIYLAAITGIDPSLYEAARIDGANRWKQTLHITLPGMRMVI
                     VLLMVLSLGNILNAGFDQIFNLYSPPVYESGDIIDTFVYRIGLLEAQYGVATAVGLFK
                     SVVSFTLISVSYFFAYKFADYRIF"
     gene            6327..7211
                     /locus_tag="CCEL_RS06285"
                     /old_locus_tag="Ccel_1254"
     CDS             6327..7211
                     /locus_tag="CCEL_RS06285"
                     /old_locus_tag="Ccel_1254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816921.1"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_015924759.1"
                     /translation="MKHRKLKKVSVFTIVNNSFLIFAALLCIIPLVHILAVSFSSSSA
                     AAAGKVVFWPVEFTLNSYNYVAKRVAFWHSMLVSVERIALGGFINLVLTILCAYPLSK
                     EKTEFRFRTFYAWAFFITMLFNGGLIPWYIVIKQMGLLDSIWALVLPGAVPVFSVILL
                     LNFFREIPKELSEAAYIDGAGHWTTLWRIMVPVSTPALATITLFSLVGHWNSWFDGLI
                     LMNKAENYPLQSYIQTIVVQRSYSMLSRQEISELATISDRTLRASQIFLGTLPIVMVY
                     PFLQKYFVKGIVLGGVKG"
     gene            7357..8835
                     /locus_tag="CCEL_RS06290"
                     /old_locus_tag="Ccel_1255"
     CDS             7357..8835
                     /locus_tag="CCEL_RS06290"
                     /old_locus_tag="Ccel_1255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="WP_015924760.1"
                     /translation="MVKMILNADIKKGKINKNIYGHFSEHLGRCIYEGLWVGEDSEIP
                     NINGFRTDVVEALKKMKIPVLRWPGGCFADEYHWVDGIGPKENRPCMVNTHWGGVVEN
                     NHFGTHEFLELCEMLGAEPYICGNVGSGTVHEMQQWVEYMTFDGKSPMADLRRANGKE
                     EPWRVKYFGVGNENWGCGGNMTAEFYADQYKRYATYVRNFGDNKIYKIAGGASVDDYH
                     WTEVLMREAGKQMDGLSVHYYTRISKDWSEQGSATEFDENHWFSVMQNALFTEELVVR
                     HSNIMDKYDPEKKVGMIVDEWGTWFAVEPGTNPGFLYQQNTMRDALVAGIHLNIFNNH
                     CDRVQMANIAQMVNVLQAVILTEGKKMLLTPTYHVFDMYKVHQGAELLSLDFESPEYA
                     YNGEKVPQLSASSSVDSEGKIHVTICNLNPSADVNIDIDLRGVKSKSSTGKIITSNAM
                     NAKNTFEETTNVTIKNFEDINLENNHISGKIPSKSVVLLEIV"
     gene            8944..9198
                     /locus_tag="CCEL_RS06295"
                     /old_locus_tag="Ccel_1256"
     CDS             8944..9198
                     /locus_tag="CCEL_RS06295"
                     /old_locus_tag="Ccel_1256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="WP_015924761.1"
                     /translation="MKDNCLKCHRNIHITHNDIEMEVKRVVKSGVPLARNEQYEKRLE
                     VCNSCKHLADGITCMICGCIVKARAMNALRICPHPSGNKW"
     gene            9246..10748
                     /locus_tag="CCEL_RS06300"
                     /old_locus_tag="Ccel_1257"
     CDS             9246..10748
                     /locus_tag="CCEL_RS06300"
                     /old_locus_tag="Ccel_1257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="WP_015924762.1"
                     /translation="MKIQNPIIWADMPDPDIIRVGDIYYMVSTTMFVMPGAPILKSKD
                     LYHWELVSYVFNTIEDNDIYQLKNGKTAYGKGQWATSLRFFNGLYYACFVCHDMQKTY
                     IYYTDDIEKSGWERYVINNVFHDMSFLFDNNRSYLIYGNGDIKIVELKDDLSGIKDGG
                     VNQLLFSTPSENIMLRCEGCRAYKINGYYYLLFIEWPNDGNSRRRVVCYRSKDLLGEY
                     DRKILLDDDMEYQNQGIAQGAIIDTPEGEWYSILFQDHGAVGRIPYLLPVSWEDDWPN
                     LGVNEKVPESFEIPFEAYDTKPLIVSDSFNHNENKLDLRWQWNHNPEEDCWSFTEHPG
                     YLRLRTQSLATDILTARNTLTQRTSGPQCAFSVELKTEGMKAGDYAGLVALQGNYGAI
                     GIRADENNLKRIIVSKKGSDGRQIEEEYRLFADSRILIKIEFDFRNSSDIAKFYYSQD
                     GIGWIKLGSDLHMTYTLDLFIGYRIGIFYYSQKAAGGFADFRNFSYESRQ"
     gene            10766..11896
                     /locus_tag="CCEL_RS06305"
                     /old_locus_tag="Ccel_1258"
     CDS             10766..11896
                     /locus_tag="CCEL_RS06305"
                     /old_locus_tag="Ccel_1258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616212.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_015924763.1"
                     /translation="MSKGAYFTKQYPNLFAELGISDEQINKKVNDTFNTMFFDPEEKI
                     YFEIGKDMGYMMDTGNNDARTEGMSYGMMMTLQMDRKDIFDRLWLFSKTYMYQNEGKY
                     QGYFAWSVSTDGKKNAEGPAPDGEEYFAMALFFAGKRWGDGKPPFDYSIQARDILKHC
                     IHQSEIVEGGEPMWDSTNHYIKFVPETPFSDPSYHLPHFYELFALLANEEDKDFWKKA
                     AEASRNYLHISCDRDTGMASEYAEFDGTPKKLFRDFQFYSDSYRVAMNIGLDAAWFSK
                     DESLGDIVDKLQSFFSENTVLGEYKAYTVKGEPFDAPAMHPVAIIATNAAGSLAAKGK
                     YRDQWVKDFWELPLRKGVHRYYDNCLYFFSLLMLAGKYKIYI"
     gene            11963..13468
                     /locus_tag="CCEL_RS06310"
                     /old_locus_tag="Ccel_1259"
     CDS             11963..13468
                     /locus_tag="CCEL_RS06310"
                     /old_locus_tag="Ccel_1259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816916.1"
                     /GO_function="GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH39 family glycosyl hydrolase"
                     /protein_id="WP_015924764.1"
                     /translation="MNKIKISDKVTGSFRDNWNFCVGTERMGIALQKEYQDTLEFVQK
                     AINFKYIRGHGLFHDDTAIYREYEIDGEMKPFYNFTYIDRIFDSFLEKGLKPFVELGF
                     MPSQLASGGQTVFYWKGNVTPPKDYDKWKNLIQALIKHFISRYGIEEVLQWPFEVWNE
                     PNLKVFWKDADESEYFKLYKVSATAIKEINKDLQVGGPAICGGADYWIKDFLEFCKRE
                     NVPVDFVSRHLYSAHMPKLQTHEFCYQDLKEPEEMLKELQTVRQMIDNSPFPHLPFHI
                     TEYNTSYNPLNPVHDTCLNAAYLARIISEAGDIVDSFSYWTFSDVFEEADVPRAQFHG
                     GFGLVALNNIPKPTFHMFSFFEQMGKDILYRDENMLATRKADGTVTITAWNPVMEKGD
                     HFNMDFSLELNLPKGEYFINRTIVNETHGNPWRTWIQMGRPRFPGKKQVEILRNAARP
                     MVISERVSCTDEILLLEFTQSKNEVSFIEIIPVADETNTYYKLDDSLIPGY"
     gene            13516..14313
                     /locus_tag="CCEL_RS06315"
                     /old_locus_tag="Ccel_1260"
     CDS             13516..14313
                     /locus_tag="CCEL_RS06315"
                     /old_locus_tag="Ccel_1260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_015924765.1"
                     /translation="MAIFQANFFAKTMKRSICFNALIPNDKYQAPDQEIKDEPKGPMK
                     SIYLLHGYSGNHTDWLTWTKIQELSLKYNIAVFMPSGENFFYVDDEEVGAYYGELIGN
                     ELVDYTRSTFHLSDKREDTFIGGLSMGGYGAIRNGLKYSERFGRIIALSSALITNQIA
                     GITPDFKGPVADYPYYRRVFGDLDRLLGGDKDPEALIRSLKEKNADIPKIYMACGTED
                     FLINENRELHEFLVSEKVEHTYIEDSGVHDWKFWNEYIDKGLAWATE"
     gene            14366..16564
                     /locus_tag="CCEL_RS06320"
                     /old_locus_tag="Ccel_1261"
     CDS             14366..16564
                     /locus_tag="CCEL_RS06320"
                     /old_locus_tag="Ccel_1261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015924766.1"
                     /translation="MKLICGDYTLIFKDGGLEVQKTGRTIYFNKRPMFVTIKTAMAIN
                     EFYNKAYSEVNIFGNSIIAKGILTAPSGSEFSFSDVYEAGDSGFKVSRNVKVLKAGDD
                     LGFSTKISFTMAESNDTHDYNCFAPGVWYRQNQFAPDFAFGKDLDCEYFWQMETRYAL
                     PLFAMQNIASGETAAISRWASDVTMRSLDIMQSENNLDAKFTIGSIGMSRPQNKTMNY
                     MYYGFEVRKEIETRTDGLSIDYVYPGCDGQMPGVNHYGGLDYNGRVKTFQRINHPVEP
                     GFEQNYSVAVNFGNYDSYQLMMRDIWRVTYDRLRDKLFDVDNELFFHNCMNILDRYTR
                     QYGDSYGLPFACQLPHMDINCVSFQFGFVGQQPGIGYQLLRYGDKENIPETFEKGVNI
                     IDFWVRTAMTESGLPNMCYNPGISTFEPYPHYIRMLADGIEAILDAYIYMKKKGDERP
                     KWREFCQKTADWLISNQNEDGSFYRAYNTDGSVRMKSKSNTPSVIRFLVQFYLVTGDE
                     RYKNAAIKAGDWSYENAYLNMEYRGGTCDNTDIQDKEAGIYAMFGFLALYDLTEEKKW
                     LEGAVGAADYTETWTYAWKFPVRAPMEKHPFNRYSISGQSIITIGGAADVYMAACSYT
                     YYRLYVITGDEHYLDFAEFIHKNTRQSSDIDGSIGYIMPGLGHESGDFTRQTLQSQYH
                     WLPWCTFVEVDPSSRLYDTFGGYEIADAQKLSSEERIRKNRIYANYVDSL"
     gene            16603..18498
                     /locus_tag="CCEL_RS06325"
                     /old_locus_tag="Ccel_1262"
     CDS             16603..18498
                     /locus_tag="CCEL_RS06325"
                     /old_locus_tag="Ccel_1262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="WP_041706618.1"
                     /translation="MNSNRPKIRLPRIISDGMVLQRNTDVKIWGWASPGEAVTVRFIG
                     KAYHAFADDSGEWFVRLEQAKAGGPYDMYIETEESLEKITIKNILMGDVWLCSGQSNM
                     EMKMDSVKDTYPDEIVHSCNDYIRHFLVPVKYDFEKPQTDLEAGIWEAANPESILDFT
                     ATGYFFALKLFEKYNIPIGLINASLGGSPAEAWLSENALREFPEHYESAKQLSNRDYL
                     DKVLREDQESAEAWYTALNQNDEGLKSNDIPFHDTEYDAPFWQNIKVPSYWEDEGVGN
                     FNGVVWFRKEIDIPSTLADKPARLVLGNIVDEDTAYINGIEVGTTPNQYITRKYSIQE
                     GLLKEGKNTIVLRVVNTAGKGGFYKDKPYQLEIGDNIIELSGEWQYMIGAKSSPMPAP
                     AFVQWRPLGLYNGMIAPVTSYSIKGFIWYQGESNTKNPGEYENLLKALIADWRQKWNM
                     GNLPFLYVQLPNFMEASEIPMESSWAELREAQRRTLSVPCTGMAVAIDLGEWNDIHPV
                     NKKDVGFRLALAAMKTAYRDKTIVAYGPMYQSGKIDGNRIILSFTDTGSGLTVKNGDR
                     PEAFAIAGADRTFVRADAEIIGDSVAVWSEKVSHPVYVKYAWADNPADANLYNLEGLP
                     ASPFTTE"
ORIGIN      
        1 atggtcaagc taaagatgag aaacagtatt ttcagccgtt tggtaataac ctttatgata
       61 ataataattc ctctttatag tttaggtatt tatatatacg gtagtagctt acgtaccgta
      121 aaaagcgaaa tatcaaaatc aactgttgcc caggcatcat tttatctgga aagtcttgaa
      181 aaagagatag agaggataaa aatactccaa tatgattgct tgaatgatga acaattgaac
      241 aagcttgcca taagatggga aatcatgagt gaatatgaaa tatcccaaac actgctccag
      301 ctaaagcaaa gacttgttgc tataaaaagc agtagtatct atgtaaacga tgtaaatgtg
      361 cacattctcc caatttcaaa gacagtttct tcaaacagcg gagtagatga tattcacatt
      421 aatgaataca atggaatata tgttccggca ggattaaagg gagcacaaat tattaactat
      481 aacggtcagt tatatctgag tacttttaaa aaatacagcg gagcaaccaa gcctttattt
      541 actattaata tagaattgaa ccagaatgca ttaaagcaag cccttgaaca attcaatacc
      601 tatgcgggaa gcggttcgat actgataaca cctacaaata ttattgcgaa taagtcgcaa
      661 gaggataatt ctcaattaat tcagagtatt ttattgagta tgaaagaacg ggatcgtgat
      721 gggactgtat ttacaaaaat tggaaataaa cagtattatg tagttcactc aaattcctca
      781 tatttaaata tgttactatt aagatatttt cctcaggaat ttatactaaa gcctctagaa
      841 aattttaaaa cctgggcgtg ggtattttca attgctgcta ttttaataat aatcatatac
      901 tcattttcaa cttataagtt tatgcatcag ccattgaatg agctggtaaa gtccttccgt
      961 aaaattgaaa atggtgattt aaaggtgttc ataagccatg attcgaataa tgaattcgga
     1021 tacctgtaca aatgttttaa tgatatggtt aaaaatctta atatgctgat agatcaggta
     1081 tataatcaaa aaatattaat gcagaaagcc gaattgaagc atttgcagtc tcaaatcaat
     1141 cctcactttc tgtataacag tttttttatg attaatacca tggctaggat aggtgatgag
     1201 aatttagtac cgtttacaaa acaccttggg gaatatttcc gttttgtaac cagaaattct
     1261 atggataatt tatcactgga ggaagaaatt aaccatgcca aagtatatac ggagattcag
     1321 ctaatgagat tttccaaaag aattcaaatt cactttggag aatgtcccaa tatgtataag
     1381 gatttgaaag taccgagact tatattgcag cccatcattg agaacgcatt tgaacatggc
     1441 cttgaaaaga aaaaaaatgg tggattattg cttgttaatt ttgaaggaaa tgaaagagaa
     1501 ttaagaatag ttgttgaaga taacggattc gatataacag attctgaact tgaaaaattg
     1561 caaaattcat tggaagataa cggactggaa attgagacaa cgggaacatt gaatatacac
     1621 agacgaataa ggctggtttt cggtgaagaa agcggcttga ttatttccag gagcgtaatc
     1681 gggggactga aggcggtatt gaaaatcgtt ttacagaagg agtagaaaaa tgtataggct
     1741 attgattgta gatgatgagg aaattattgt aaacggatta tatgagatat tccgaagctt
     1801 aaaaaatctt gatctggatg tatacagggc atattcgggc gaagaggcgg ttgagtggct
     1861 aagcagaaca agaatggata ttgttttaac agatattaga atgccggaaa tggatggttt
     1921 gcagttattg gatgtcatat atagaagatg gcctcaatgc aaagtaatat tcctaacagg
     1981 gtataacgaa tttgaatatg tatataaagc aattcagcac cagggtgtaa gctatatact
     2041 aaaaacagaa gataatgaca aggtaataag tgttgttgaa aatgccatag aggagataaa
     2101 aaaagaaata aaaacagaag acttgattca taacgccaag gagcaaattg aacttgcgtt
     2161 gggtctcttc caaaaggatt actttattca tctgctccac gaagagaatt catcagatgt
     2221 aaataaagcc cagtttgaac aactggcaat tccaatgtac cctgatcatc ctgtaattct
     2281 gctgcttgga catattgaca gcattccggg taatctgtcc tactgggata agatacagta
     2341 tataaattct attaaagtga ttattactaa aaatctgaat accaatatca ggtccatatg
     2401 cattttggat gatagatata ggtttatttt tttcatacaa ccaaatgagc ttatgacaac
     2461 aggtttcagt aaaactgaag aaaatgcttt ttatgataag gctatttctt ttgtaaaggg
     2521 tacccttgaa gtaatacaga ctgcgtgtat ggagtcgttg aatgcctcta taagcttttc
     2581 cctcagcgga gaaccctgta actgggaaga cgtatctaaa aagtattttt ctcttaatca
     2641 actgctgaac tataaaatag gcacagggac agaaatgcta ttgattgata atgaattaaa
     2701 aaataatatt ctagcgtcag gtacagaagt acctgagctg gacacaaatg atgagccatt
     2761 ggaaattctt ttaagaagga aaaatatgga cctaattcag caatacatgg agtctggcca
     2821 aaaagaaaaa tattttgagg tattggagga attattaact ccaataaaat cagtcaaaag
     2881 taaaaacagc aatttagcca ttgaagcata ttccatggta tcgctcagta tcctttcata
     2941 tatcaatcgt tggaaaataa cggagaaact ggcatttcat ataggtctta atagccttat
     3001 gaggattgac aaacatgaaa catggtcaga tgcagtaaag tatctgataa atatatctga
     3061 tataatattc caactccaaa ccgacgaaca gaagaagagg gcggataatg cagttgattt
     3121 cgttcagagt tttataaatg agcatttgag tgaagatctt tctttggtaa gacttgcaga
     3181 acaggtttac ttgaacccat catatttgtc acgcctctat aagcaggtta caaataccaa
     3241 cctttctgac tttattgaca acgcccgtat agagcgggca aaagagcttc tgaaaaaaga
     3301 aaaggtgaag ataaatgaag tagcaaaagc cgtagggtat gaaactgcag catcctttac
     3361 aaggttcttc aagaaactaa tggggtgttc tccacaggaa tatcatgata ccatgctgtc
     3421 aggtaaataa atgataacaa ggtaaacaaa agatacgaaa ataatgaata taataaaaaa
     3481 caagtaaaca aaagataaca aaagtaaaaa tgtgtgaatg gtgttgttgc tgacctcaat
     3541 ctataatata gatgaggcct caaataaata tatattagga ggaaacaaat gagattcaat
     3601 tacttaaaaa aagctgtatc attagcagta gttttatctt taacagcttc aatcgttgta
     3661 gcttgcggta gtcaaaacaa tacatccgac agcacagcat catcatctca aagtactact
     3721 tcttcagtag caaataagga tcctttcgga aaatatagtc ctgagattga tatttctttt
     3781 gttcgtggaa tagacgatga ccttgcagca aacatattgc caaagacacc gggggaaact
     3841 cttgaagaca atcgttggac gaagttatat aaagatgaat taggtataaa tgtaaaatat
     3901 gcttggacag taaaaggaaa tgaaacatca gatgcttata cacagaaaat caatgtaact
     3961 ctggcatctg gagaactccc tgatgtagtt cttgtaaatc cttctcagct taaacaactg
     4021 gttgattcag atatgataga ggatatgacc caatattata atgattatgc ttcagaggat
     4081 ttcaagaaac ttatgacaga agaaggaacc ggtaatattg attcagctat gtttgatggt
     4141 aaaatgatgg ctattccgga acctgtttca actaatgaaa ccgcacacta tctatggatt
     4201 agaaatgact ggttgaaaaa attaaaccta caggcaccta agactatgga tgatgtttta
     4261 aaaatatcag aagcatttac aactcaagac cctgatggaa acggaaagaa cgatactttc
     4321 ggacttccga tcacaaaaga tatctacagc ggatgtatgg gaacagaagg cttctttgca
     4381 ggatatcatg catacccgaa tatgtggata gaagatgatt caggtaaaat agtatggggt
     4441 agtactttac ctgaaacaaa ggtagcactc cagaaattgg ctgacatgta caagagcggc
     4501 cagcttgaca aagaatttgg tgtcaaggat ggaggtaagg ttgccgagac tatagcagca
     4561 ggtaaagtag gtataaacta tggtcaacag tggaacccaa tgtatcctct aataagcaat
     4621 tttaacaatg ataaaaatgc cgattggaca gcatatccta ttgtttcaaa tgatgataaa
     4681 aaggttatgg tacctttaaa gttcaatcag acaagaatat ttgcagtaaa aaaaggatat
     4741 gaacatccag aagcacttgt aaaactgttc aatgcacatg ttgagaagaa ctggggtaaa
     4801 acagcagact tcaacaagta ctatatgccg gttgagaacg gcggtgtagg agtttggaaa
     4861 ttctctcctg tgtgccctgc tcctgtattc aagaaccttg aagcattcgt ggcaattgat
     4921 gaagccagaa ataacaatga cttcagcaag ctgacaggtg agcctaaaat tatccaaggt
     4981 aatatagagg cttatgctaa aggcgacact tcacaatggg gatgggaaaa gatttacggt
     5041 aaagaaggtg cattccgtaa tatggttgag tacaagaaga atgatcagct gttaaaagaa
     5101 aagtttgttg gagctccaac tacaacaatg gctgaaaaga aaacaacact tgaaaaaatg
     5161 gaaaaagaag tatttataaa aattataatg ggtgcagctc ctattagtga attcgacaag
     5221 tttgttagcg attggaataa acttggtggt gctgatatga caaaggaagt taacgaatgg
     5281 tatgactcag ttaaatccaa gtaatattaa ctagttaaca tagaaggaaa ggatacagcc
     5341 gtttcatatc ctttccttct cgtttactag gcaagatttt aaatctatac caagtgggga
     5401 ggaataagct tgaaaaattt aaacataaaa aaacaactac ctttgcatct catgatcctt
     5461 ccgggtttta tcactttatt gatattcagt tatctgccaa tggcgggcat tgttatagcg
     5521 tttcagaaat ttatacccgc aaaaggcttg ttcggggatc aaaaatggat aggctttaag
     5581 aatttcgaat atgtcctgaa tatgccgaat tttgacaaca taatgtggaa taccatatcc
     5641 atagctatgg caaaaatcat actaggactt attattccga taatatttgc aattctaatc
     5701 aatgaagtta caaataatgc tttaaaaaga ggtattcaga cacttatata ccttccgcac
     5761 tttttatcat gggttgtact aggaggtata ctaattgata tactgtctcc gtccggcgga
     5821 attttaaact catttataaa gtcccttggt tttgaaccta tattttttct tggagataat
     5881 aaatggtttc ctagcacaat ggtattgacg gagacttgga aagaatttgg ctacggtaca
     5941 ataatctatc ttgcagcgat taccggaatt gaccccagtt tatacgaagc agctcgtata
     6001 gatggagcta acagatggaa gcaaacctta cacattactc ttccgggtat gaggatggtt
     6061 atagttcttc ttatggtgtt aagtcttgga aatattttaa atgccggttt tgaccagatt
     6121 ttcaatttat atagtccccc tgtctatgaa agcggagaca taatcgacac ctttgtttac
     6181 agaataggtc tattggaagc ccaatatgga gttgccacag cggtaggact attcaaatca
     6241 gtagtctcgt ttacacttat ttcagtatct tacttctttg cttataaatt tgcagattat
     6301 aggattttct aaagggggtt tattaaatga agcatagaaa acttaagaaa gtcagtgtat
     6361 ttacaatagt aaataacagt tttctaatat ttgcagcatt actttgtatt ataccgcttg
     6421 tgcatatatt ggcagtatca ttcagctcca gctccgcagc cgcagcaggt aaggttgtat
     6481 tctggcctgt tgagtttaca ttaaattcat ataactatgt tgcaaaaagg gttgcttttt
     6541 ggcattcaat gttggtttca gttgaaagaa tcgcactggg tggatttatt aacctggtac
     6601 ttactatatt atgtgcgtat cccttatcaa aggaaaagac ggagtttcgt ttccgtacat
     6661 tttacgcatg ggcatttttt ataacaatgt tgtttaacgg aggcttgatt ccatggtata
     6721 ttgtaatcaa gcagatggga ttacttgata gtatatgggc tttggttctt ccaggtgcag
     6781 ttccggtttt cagtgtaata ctattactaa attttttcag ggaaataccg aaggaattaa
     6841 gtgaagcagc ttacattgac ggagcaggcc actggactac actctggaga ataatggtac
     6901 ctgtatctac cccagctttg gcaacaataa ctctgttctc attggtaggt cactggaaca
     6961 gctggtttga cggtctgata ttaatgaata aggcggaaaa ttaccctctt caaagctata
     7021 tacagactat tgtagtgcag agatcctaca gtatgctttc aagacaggaa atttccgaac
     7081 tggcaactat ttcagacagg acattaagag cttctcagat tttcctggga accttgccta
     7141 tagttatggt atatcctttc ctacagaaat attttgtaaa gggaatagtt ctcggtggtg
     7201 taaaaggata acagcaaaaa ttgagatgat atattctcaa tgaagggaaa acacgtcttg
     7261 tgctatgcaa gagaagcaca ggatcaaatg taatattgaa gggatgtaaa atattatttt
     7321 ttagaaatat ttcaggtgtt aaggagagtg aacgagatgg taaaaatgat tttaaatgca
     7381 gatataaaaa aaggaaagat aaataaaaac atatacgggc atttttcgga gcatctgggt
     7441 agatgtatat atgaggggtt gtgggttggc gaagactcag aaatacctaa tataaacgga
     7501 tttcgcacag acgttgtcga agccctcaaa aagatgaaga tacctgtgtt aagatggccg
     7561 ggcggatgct ttgcagatga atatcactgg gtagacggaa taggacctaa agaaaacaga
     7621 ccttgcatgg ttaacaccca ttggggcgga gtcgtagaaa ataaccattt tggaactcat
     7681 gaatttctgg aactttgcga aatgcttggt gcagaaccat atatatgcgg taatgttgga
     7741 agcggcacag tacatgaaat gcagcagtgg gttgaataca tgacctttga cggaaaatct
     7801 ccaatggctg atttaagaag ggcaaacggc aaggaggaac catggagggt taagtatttc
     7861 ggcgttggta acgaaaactg gggctgcggc ggaaacatga ctgctgagtt ctacgcagat
     7921 caatataaaa ggtatgcaac atatgtaaga aacttcggcg ataacaagat atacaaaata
     7981 gccggaggtg cttcggtaga tgactatcac tggaccgagg tgcttatgag agaagcagga
     8041 aagcaaatgg acggtctcag tgtccattat tatacaagaa tctcaaagga ctggtctgag
     8101 caggggtctg caactgaatt tgatgaaaat cattggtttt ctgtaatgca gaatgctttg
     8161 tttacggaag agttggtggt acgtcattca aacattatgg ataagtatga tcctgagaaa
     8221 aaagtaggta tgattgttga tgaatggggt acatggtttg ctgttgaacc cggaacaaat
     8281 ccgggattcc tgtaccagca aaatacaatg agggatgccc ttgttgcagg aatccacctg
     8341 aatatcttca ataaccactg cgacagggtt cagatggcaa acatagcaca aatggtaaat
     8401 gtccttcagg cagtaattct aactgagggt aaaaagatgc ttctaacgcc cacctaccat
     8461 gtatttgata tgtacaaggt acatcagggt gcggagttgc tttctcttga ctttgaaagt
     8521 ccggagtatg cctataacgg tgaaaaggta cctcagctga gtgcttcttc ttctgttgac
     8581 agcgaaggaa aaattcatgt aaccatttgc aaccttaacc catcagcaga tgttaatatt
     8641 gatattgacc tgcgaggagt aaagtctaag agtagcacag gtaaaataat tacctccaat
     8701 gccatgaatg caaaaaacac atttgaagaa acaactaatg taactataaa gaattttgaa
     8761 gatatcaacc ttgaaaataa ccatatatca ggaaaaatac cttctaaatc agttgtattg
     8821 cttgaaattg tataattagg gcagtattgt gacttattta actgaatatg gtaaaatctc
     8881 attagaatgt cccggcagaa tataattaat gccgggatat ttagtagtta aaggagggtg
     8941 gcggtgaagg ataattgctt aaaatgtcat agaaatatcc atattactca taatgacatt
     9001 gaaatggaag taaaaagggt agttaaatcg ggcgtaccct tggcaaggaa tgaacaatat
     9061 gaaaagagat tggaagtttg taacagctgt aagcatttag cggatggaat cacttgtatg
     9121 atttgcggat gcattgtaaa ggctagggca atgaatgctt tacgtatttg ccctcacccg
     9181 tctggcaaca agtggtaatt acttagagag gtaaatcaaa atcctaaaca agagaggact
     9241 ttgccatgaa aattcagaac cccattatat gggcggatat gccggacccg gacattatac
     9301 gtgtaggtga tatatattat atggtatcta caaccatgtt tgtaatgcca ggagctccaa
     9361 ttttaaaatc aaaggattta tatcattggg aacttgtttc atatgttttc aatactattg
     9421 aggataatga catatatcag ctgaaaaatg gcaaaacagc atatggaaag ggtcaatggg
     9481 caaccagttt gagatttttc aacggccttt actatgcttg ttttgtatgc catgatatgc
     9541 aaaagacata tatttattat acggatgata tagaaaaaag cggttgggaa cgttatgtga
     9601 tcaataatgt tttccatgat atgtcgtttt tgtttgataa taacaggtca tatttgatat
     9661 atggcaacgg ggacattaaa atagttgaac taaaagatga tttatcagga ataaaagatg
     9721 gaggggtcaa ccaactcctg ttcagtactc cctctgaaaa tataatgctc cgttgtgagg
     9781 gctgcagagc ctacaaaatt aatggctact attatttgtt attcattgaa tggcctaatg
     9841 acggtaacag cagaaggcgt gttgtgtgtt acaggtcaaa ggatttactt ggagagtacg
     9901 atagaaaaat actccttgat gatgatatgg agtatcagaa tcagggaatt gctcaggggg
     9961 ctataattga tacacctgaa ggagaatggt attcaatttt gtttcaggat catggggcgg
    10021 taggcagaat tccttattta cttcctgttt catgggaaga tgactggcct aatttgggag
    10081 ttaatgaaaa ggtgccggaa agttttgaga ttccttttga ggcctacgat accaaaccat
    10141 taatagtaag tgatagcttt aatcataacg aaaataagct ggatttaagg tggcaatgga
    10201 atcacaatcc tgaggaggat tgttggtcat ttacagaaca tccgggctat ttgagactgc
    10261 gtacgcaatc cttggccact gatattttga ccgccaggaa tacattaact caaaggacaa
    10321 gcggtcctca atgtgcattt tcagtggagc ttaaaactga gggaatgaaa gcaggggact
    10381 atgccggact tgttgcgtta cagggtaatt atggggccat aggaataaga gccgacgaga
    10441 ataatttgaa aagaattatt gtttctaaaa aaggcagcga cggaagacaa atagaagaag
    10501 aatacaggct gtttgcagac agtagaatat taataaaaat tgaattcgat ttccgaaaca
    10561 gcagtgatat tgcaaaattt tattattccc aggatggtat cggatggata aaattgggca
    10621 gtgatttgca tatgacatat acccttgacc tttttatagg ctatcgtatc ggtatattct
    10681 attattctca gaaggcagcc ggagggtttg ctgatttcag aaatttttca tatgaatcac
    10741 gacaatgaac aaaaggagag taattatgtc aaaaggagct tattttacaa aacagtaccc
    10801 taatttattt gccgaactgg gtatttctga cgaacaaatt aataaaaagg ttaatgacac
    10861 ttttaacact atgttcttcg atcccgagga gaaaatttat tttgaaatag gtaaagacat
    10921 gggatatatg atggatacag gtaataatga tgcacgtaca gagggtatga gctatgggat
    10981 gatgatgact ctgcaaatgg atcggaaaga catttttgac cgcttgtggt tattttccaa
    11041 aacatatatg tatcaaaacg agggaaagta tcagggctat tttgcatggt cggtatctac
    11101 cgacggaaag aaaaatgccg aagggcctgc acctgacgga gaagagtatt tcgctatggc
    11161 tcttttcttt gcaggcaaaa gatggggtga cggtaagccg ccctttgact atagtattca
    11221 agccagggat attttaaaac attgtataca ccagtcggag attgttgaag gtggagaacc
    11281 tatgtgggat agtaccaacc attatataaa atttgttcct gaaacgcctt tctctgatcc
    11341 gtcttaccat ctgccccatt tctatgagct ttttgcgctt ctggctaatg aagaggataa
    11401 agacttctgg aaaaaagctg ctgaggcaag tcgtaattac ctgcatattt catgcgacag
    11461 ggacactggg atggcatcgg aatatgctga atttgacggt actcccaaaa agctgttccg
    11521 tgattttcag ttttattctg attcataccg cgttgcaatg aatataggat tggatgcggc
    11581 gtggttcagt aaggacgagt cattagggga tatcgttgac aagcttcagt ccttctttag
    11641 tgaaaatacg gtgttaggcg aatataaggc ctatactgtt aaaggtgagc cttttgatgc
    11701 tcctgccatg caccccgttg caattatcgc tacaaatgcc gccggttcac ttgctgctaa
    11761 agggaaatac agagatcagt gggtaaagga tttctgggag cttccattaa gaaaaggagt
    11821 tcataggtat tatgataact gtctgtactt tttcagttta ctgatgctgg caggaaaata
    11881 taaaatttac atctaagtat aatatcatta attaaatctc aaacctatat attaatagtg
    11941 taaaaaatac gagggggata atatgaacaa aataaagatt tcagataagg ttacaggaag
    12001 tttcagggat aattggaatt tctgtgtagg tacagagcgt atggggatcg ctcttcaaaa
    12061 ggaataccag gatactctcg agtttgttca gaaggcaata aatttcaaat atatacgagg
    12121 acatggtctt tttcatgatg acactgccat ctacagggaa tatgaaatag acggagaaat
    12181 gaaacctttt tataacttta catacataga caggattttt gactcattcc ttgaaaaggg
    12241 tctcaagcct tttgtagagc tgggatttat gccgtcacaa cttgcatcgg gaggacagac
    12301 tgtattttat tggaaaggga atgtcactcc tccaaaggat tatgacaagt ggaaaaatct
    12361 cattcaagct ttgataaaac actttataag tagatatggt atagaggagg ttctccagtg
    12421 gccttttgag gtctggaacg agccaaattt gaaggtattc tggaaggatg ccgatgagtc
    12481 cgaatacttt aagctttata aggtgtcggc aacagctatt aaggaaatca ataaggattt
    12541 acaggtaggg ggccctgcta tatgcggagg tgccgactac tggataaagg attttttgga
    12601 gttctgcaaa agagaaaatg ttcctgtaga ttttgtttcc cgccatttat attctgcaca
    12661 tatgccaaaa cttcagacac atgaattttg ttatcaggat ttgaaagaac ctgaggaaat
    12721 gcttaaagaa ttacagacag tcaggcaaat gattgacaat tcaccatttc cacacctgcc
    12781 atttcacata acagaataca acacctcata caatccatta aaccctgttc atgatacatg
    12841 tctcaatgct gcgtatctgg cacggattat aagtgaagcc ggggatattg tggattcatt
    12901 ttcatactgg accttcagtg atgtatttga agaggcagat gttccaagag ctcagtttca
    12961 cggagggttt ggccttgttg cccttaacaa tattccaaaa cctacttttc atatgttcag
    13021 cttttttgaa caaatgggta aggatatatt gtacagagat gaaaatatgc tggcaacaag
    13081 aaaagctgac ggtacagtga caataaccgc ttggaaccct gtaatggaga aaggtgacca
    13141 ttttaatatg gatttctcac tggaactgaa tctgccaaaa ggagaatact tcatcaatcg
    13201 gactattgta aatgaaactc atggtaatcc atggagaact tggatacaaa tggggaggcc
    13261 tagattcccg ggtaagaagc aggttgaaat attaagaaat gctgcacgcc ccatggtaat
    13321 ctccgaaagg gtaagctgta ccgatgaaat attgcttctt gagtttacac aaagtaaaaa
    13381 cgaggtatct tttattgaaa tcattcctgt agcagatgag actaacactt actataaact
    13441 tgatgacagt ttgattcccg ggtattaaaa tatattatat acttataata caaattttat
    13501 aaaaggagaa ttattatggc aatttttcaa gcgaattttt ttgctaaaac gatgaaaaga
    13561 tcgatatgtt ttaatgcttt gataccaaat gataaatatc aggcacctga tcaggagatt
    13621 aaagacgaac caaaagggcc tatgaaatcc atttatctac tgcatggcta ttccggcaat
    13681 catacggatt ggttgacatg gactaaaata caggagctgt ctctaaaata taatattgcg
    13741 gtatttatgc cttccggtga aaatttcttt tatgtggatg acgaagaggt tggagcatat
    13801 tatggagaat taatcggaaa tgagctggtt gactatacac gcagcacatt ccatttatct
    13861 gataaaagag aggatacttt tataggtgga ttatccatgg gtggttacgg tgcaataaga
    13921 aacggtttaa aatactctga aagattcggc cgtataattg cactgtcctc agcacttatt
    13981 accaatcaga ttgccggaat tacccctgat ttcaagggcc ctgttgcaga ctatccttat
    14041 tacagaagag tatttggaga tcttgaccgg cttttgggag gtgacaaaga ccctgaggca
    14101 ctaatccgta gccttaagga aaaaaatgca gacataccta agatatatat ggcttgcgga
    14161 acagaagatt ttcttataaa tgaaaataga gagctccatg aattccttgt ttcagaaaaa
    14221 gtagagcata cgtatattga agacagcggt gttcacgatt ggaaattctg gaacgagtat
    14281 atagacaagg gacttgcttg ggctactgaa tgaggatgaa aataacaata ccgaagatta
    14341 cttaaatacc agaggaggaa ttcccatgaa gctgatatgc ggcgactaca cacttatatt
    14401 taaagatggc ggtctggagg ttcaaaaaac cggtaggacc atatatttca acaaacgtcc
    14461 catgtttgtg actattaaga cagcaatggc gattaacgaa ttttataata aggcctacag
    14521 cgaggtaaat attttcggta atagtattat tgcaaagggt atactgaccg caccctcagg
    14581 atcagaattt tccttttcag atgtatatga agcgggagac tctggtttca aggtcagcag
    14641 gaatgtaaag gtgctgaagg ctggggatga ccttggcttt tcaaccaaaa tatccttcac
    14701 gatggctgaa tcaaatgata cacatgatta taactgcttt gcaccgggag tatggtacag
    14761 acagaaccaa tttgcaccgg attttgcatt tggaaaggat ctggattgcg aatacttctg
    14821 gcaaatggaa acacgctatg ctttgcctct atttgccatg caaaatatag cgtccggtga
    14881 aacagcggct atatctcggt gggcatcgga tgtaacaatg cggtctttgg atattatgca
    14941 atcagaaaat aatctcgatg ccaaatttac aataggttca attggtatga gcagaccgca
    15001 aaataagacc atgaactata tgtattacgg atttgaggta cgaaaggaaa tcgaaacaag
    15061 aaccgacggg ctctcaattg attatgttta tcccggctgt gacggacaga tgcccggagt
    15121 aaaccactat ggaggtcttg actacaacgg cagagtaaaa acctttcaac gtattaatca
    15181 ccctgtagaa ccgggctttg agcagaatta ctccgttgca gtgaattttg gaaactatga
    15241 cagctatcag cttatgatga gagatatctg gagagtgact tatgacagat taagagacaa
    15301 attattcgat gtggacaatg aactcttttt ccataactgt atgaatatac ttgacaggta
    15361 tacaaggcag tacggtgatt cttacggact cccatttgcc tgccagcttc cccatatgga
    15421 tataaattgt gtatcctttc aattcggttt tgtagggcaa cagccgggaa tcggctatca
    15481 gcttctgagg tacggagata aggaaaacat cccggaaacc tttgaaaaag gtgtaaatat
    15541 cattgatttc tgggtcagga cggccatgac agaatcaggc ttacccaata tgtgctacaa
    15601 tcccggcata agtacttttg aaccgtatcc ccattatata agaatgcttg cagatggtat
    15661 tgaggccatt ctggatgcat acatctatat gaaaaagaag ggggacgagc ggcctaaatg
    15721 gagagaattc tgtcagaaaa cagctgattg gcttattagt aatcaaaatg aggacggcag
    15781 cttttaccga gcatacaata ccgatggctc tgtaaggatg aaatccaagt caaatactcc
    15841 aagcgtaata cgttttctgg ttcagttcta ccttgtgaca ggtgacgaaa ggtataaaaa
    15901 tgcagccata aaggctggag actggtctta tgaaaatgct tacctgaaca tggagtacag
    15961 aggcggtaca tgcgacaata ccgatataca ggacaaggaa gcaggcattt atgcaatgtt
    16021 tggctttctg gctctctatg atttgactga agaaaagaaa tggcttgagg gtgccgtagg
    16081 tgctgctgac tacactgaga cttggaccta tgcatggaaa ttccctgtac gtgctccaat
    16141 ggagaagcat cccttcaata ggtattccat cagcggtcag agtattataa caatcggtgg
    16201 tgcagcagat gtatatatgg ccgcttgctc gtacacatac tacagattgt acgttataac
    16261 cggggatgaa cattatctgg attttgccga gttcattcac aaaaatacca gacaatccag
    16321 cgatatagac ggaagtattg gttatataat gccggggttg ggccatgaaa gcggagactt
    16381 tacgaggcaa actttacaaa gccagtacca ttggcttcca tggtgtactt ttgtggaggt
    16441 cgacccatca tccagacttt acgatacctt tgggggatat gagattgctg atgcacaaaa
    16501 actcagttca gaggaacgca ttcggaaaaa ccgaatatac gctaattatg ttgatagttt
    16561 ataaatatag taattaatac tgttttgaaa gtgggaatta tattgaattc taatcgccct
    16621 aaaataagat tacccaggat tataagcgac ggcatggtat tacaaagaaa cacagacgta
    16681 aagatttggg gttgggcatc accgggtgaa gccgttactg tccgctttat aggcaaagcc
    16741 tatcatgctt tcgcagatga tagcggagag tggtttgtaa gacttgaaca agcgaaagca
    16801 ggcgggccgt atgatatgta tattgaaacg gaagagtccc ttgaaaagat aaccataaag
    16861 aatattctta tgggcgatgt ctggttgtgc tccgggcaat caaacatgga aatgaaaatg
    16921 gattcagtga aggacaccta ccctgatgaa atcgtgcact cttgtaatga ttatataaga
    16981 cattttcttg ttcctgtgaa atacgacttc gaaaaacctc aaactgattt ggaagcagga
    17041 atctgggagg cagccaatcc tgaaagtata ttggatttta cagctacagg ttatttcttt
    17101 gccttaaagc tgtttgaaaa atataacatt cctattggtc tgattaatgc cagcctagga
    17161 ggctcaccag cagaagcatg gctcagtgaa aatgcattaa gggagttccc tgaacattat
    17221 gaatcagcaa aacaactcag caaccgggat tatctggata aagtgctcag ggaagatcaa
    17281 gagtccgcag aagcatggta cacagcgtta aaccaaaacg atgagggatt aaaaagcaat
    17341 gatataccat tccatgatac agaatatgat gcccccttct ggcagaacat aaaagtacct
    17401 tcatattggg aagacgaggg tgtgggaaac ttcaacggtg tcgtctggtt caggaaggaa
    17461 atcgatattc ccagcactct tgcagataaa cctgcaagac ttgtacttgg aaatattgtt
    17521 gatgaagata ctgcctatat caatggtata gaggttggaa caacgccaaa ccagtatatt
    17581 acgagaaaat acagtataca ggaaggcttg ctcaaggaag gtaaaaacac tattgtgctc
    17641 agagtagtaa ataccgcggg taaaggggga ttttacaaag ataagcccta ccaactggaa
    17701 ataggggata acatcattga gttaagcggt gaatggcagt atatgattgg tgcaaaaagt
    17761 agtccaatgc ctgctcctgc cttcgtacag tggagaccgt taggtttgta caacggaatg
    17821 attgccccgg ttaccagtta ctcaatcaaa ggtttcatat ggtatcaggg ggaatccaat
    17881 accaaaaatc cgggagaata tgaaaatctt ctgaaagcac tgattgcaga ctggagacag
    17941 aaatggaata tgggaaatct gccgtttttg tatgttcagc tgccaaactt tatggaggcc
    18001 tcggaaatac ctatggaaag cagctgggcg gagttgaggg aagctcagcg aaggacactt
    18061 tctgtacctt gtacgggaat ggctgttgca attgatttgg gtgaatggaa cgatattcat
    18121 cctgtaaata aaaaggatgt gggatttcgc ttggcacttg ctgccatgaa aactgcatat
    18181 agggacaaaa cgattgttgc atacgggcca atgtatcagt ctggcaaaat cgacggaaac
    18241 agaataattc tatccttcac tgatacggga agcgggttga ctgttaaaaa cggagaccgt
    18301 ccggaagctt ttgctatagc aggagcagat aggacatttg tcagggctga tgccgagata
    18361 ataggtgaca gcgtagcagt gtggagtgaa aaggtttctc accctgttta tgtaaagtat
    18421 gcatgggctg ataatccggc ggatgccaac ctctataacc ttgaaggctt gccggcttca
    18481 ccttttacaa cggagtag
//
