LOCUS       NC_020238               9680 bp    DNA     linear   CON 08-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. HD73, complete
            sequence.
ACCESSION   NC_020238 REGION: 5336873..5346552
VERSION     NC_020238.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603412
            Assembly: GCF_000338755.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. HD73
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. HD73
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Liu,G., Song,L., Shu,C., Wang,P., Deng,C., Peng,Q., Lereclus,D.,
            Wang,X., Huang,D., Zhang,J. and Song,F.
  TITLE     Complete genome sequence of Bacillus thuringiensis subsp. kurstaki
            strain HD73
  JOURNAL   Genome Announc 1 (2), e0008013 (2013)
   PUBMED   23516207
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Liu,G., Song,L., Shu,C., Wang,P., Deng,C., Peng,Q., Lereclus,D.,
            Wang,X., Huang,D., Zhang,J. and Song,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2013) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan West Road Haidian
            District, Beijing, Beijing 100094, China
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP004069.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The bacteria is available from Fuping Song (fpsong@ippcaas.cn).
            
            ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.3
            Sequencing Technology :: Sanger dideoxy sequencing; 454; Illumina
            ##Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000338755.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 03:12:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,160
            CDSs (total)                      :: 6,015
            Genes (coding)                    :: 5,686
            CDSs (with protein)               :: 5,686
            Genes (RNA)                       :: 145
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 329
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 155 of 329
            Pseudo Genes (incomplete)         :: 186 of 329
            Pseudo Genes (internal stop)      :: 48 of 329
            Pseudo Genes (multiple problems)  :: 50 of 329
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     HD73"
                     /mol_type="genomic DNA"
                     /strain="HD73"
                     /serovar="kurstaki"
                     /db_xref="taxon:1279365"
     gene            1..2676
                     /locus_tag="HD73_RS27950"
                     /old_locus_tag="HD73_5607"
     CDS             1..2676
                     /locus_tag="HD73_RS27950"
                     /old_locus_tag="HD73_5607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820936.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="WP_000820970.1"
                     /translation="MKRIDLIFNAIQKGNYTEGVTASELATFLQLDRANVSSDLNKLV
                     KDKRLLKTNTRPVRFYIETNTSLETHSKDITSLDTFAIENTSLKIAIEKAKASILYPP
                     NGMHTLLLGETGVGKSMFASLMHEYAIEVEQLPKDAPFIVFNCADYANNPQLLLGQLF
                     GIKKGAYTGASDQKGLIEKAHEGILFLDEVHRLPPEGQEMLFTFIDRGVYRRLGETEN
                     ERKAQVLIITATTEEPNSFLLKTFTRRIPMTVTLPPLRERTHKERFALLQRFFTNEAI
                     RLRKDIHVSPNAMRAFVFYNCPNNIGQLKTDVQIACAKAYSDLVTKKRDSVYVSSSDL
                     PWYMKEGLFIERKSRHLYQIPNETFVFTGEEGWSNHKTEDEKRSSIYDYIDYKYEELQ
                     ARGIEEEELELLIENDVQSFFVQYFNQISKKTSHENVFKIVDRNIVSVCEKIAELAEK
                     HLSKTFDEKVFLALSLHVQTTLQRLQSGKQIHHPQLNQIRTKYKEAFSVAMQCIQLLE
                     EELQITMPIDEAGFLTMFFVVDPIPASQTEVKVLILAHGNGIATEMANVANELLGIEE
                     VTGIDMPLHESPKDFLERVKVYMKTLQNVNGLLLLVDMGSLAYIGDILETEFKIPVRV
                     LSMTSTPHALEAARKAQLGYSLDALYETVKNLTPFYLNVQEEKKKPLSPMKSVILTAC
                     LTGEGSALAIQKMLENYLRFDKDLIEIIPISIVHEKDLTKMIENIKKERNIICIVTNF
                     DVQVPCLTYHFQDIVNYTAIQPIQELITYEETYAKMADILEQQMQRNDGALLIKTIRY
                     ALNTIQELISLQLTPDSLMGVILHMSCMVDRLQKGESLLPHPDKEKRRQDEYWMYMKV
                     KKALQPIENTFEIQIPDDEVFYVMDFFIKNQPVKN"
     gene            complement(2981..3394)
                     /gene="exsM"
                     /locus_tag="HD73_RS27955"
                     /old_locus_tag="HD73_5608"
     CDS             complement(2981..3394)
                     /gene="exsM"
                     /locus_tag="HD73_RS27955"
                     /old_locus_tag="HD73_5608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016118901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium regulatory protein ExsM"
                     /protein_id="WP_000207046.1"
                     /translation="MTTHFFARLTGKREVPPVNTEAFGVAEFIFSDDLTKLHYRVILK
                     NIEKVTSCQIHLGKSTQIGPVVLYLYGPLEQGISLNEGSITGVVNVEDFEGPLQGKSF
                     VHLLQEIIQANVYVNVHTKSQKRGEIRGRVRKVKK"
     gene            3582..4763
                     /locus_tag="HD73_RS27960"
                     /old_locus_tag="HD73_5609"
     CDS             3582..4763
                     /locus_tag="HD73_RS27960"
                     /old_locus_tag="HD73_5609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="WP_000856213.1"
                     /translation="MKTNKYTFHTLLEIFLVSFKLGLTSFGGPVAHLGYFHHEYVQKR
                     KWMDERSYGDLVALCQFLPGPASSQVGMGVGLLRGGLLGAIISWIGFTLPSVLVLVFF
                     ASFLNQFDLGSAGWIHGLKLVAVAIVAHAIWGMARKLTPDRNRATIAIVTAAIALLWP
                     SSWTQVILIIICGFIGWFLYRNQPISQSQNIRVPISKKIAVSCLVLFFGLLLLLPILR
                     PFSYYIALFDSFYRSGALVFGGGHVVLPLLEGEFVQNGMMTKEQFLAGYGLTQAVPGP
                     LFTFASYIGAVLNGTLGAIIATIAIFLPAFLLVIGVLPFWNNVRKISFIQGALLGVNA
                     AVVGILIAAFYDPIWTSTIINASDFVFASLLFCLLAFWKAPPWVIVILGAFGGYILSI
                     L"
     gene            complement(4802..5506)
                     /gene="chbG"
                     /locus_tag="HD73_RS27965"
                     /old_locus_tag="HD73_5610"
     CDS             complement(4802..5506)
                     /gene="chbG"
                     /locus_tag="HD73_RS27965"
                     /old_locus_tag="HD73_5610"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593491.1"
                     /GO_function="GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /GO_process="GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="WP_000593487.1"
                     /translation="MIKLIVNADDFGLTEGTNYGIIDGHINGLVNSTTMMMNMPGTEH
                     AVRLAKEYNLLGVGVHLVLTAGEPLLGDVPSLVGSDGSFHKQSVVREGNINPEEVERE
                     WTAQIEKFLSYGLTPTHLDSHHHVHGLPILHDVLERLAAKYNVPIRRCGEDRAVHPFS
                     DVFYSDFYADGVTEDYFMKLKERVQGEQTVEIMVHPAYIDPELVKRSSYVMDRVKELR
                     ILTESELPEGIELVKF"
     gene            complement(5556..6881)
                     /gene="celF"
                     /locus_tag="HD73_RS27970"
                     /old_locus_tag="HD73_5611"
                     /gene_synonym="chbF"
     CDS             complement(5556..6881)
                     /gene="celF"
                     /locus_tag="HD73_RS27970"
                     /old_locus_tag="HD73_5611"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="WP_000145762.1"
                     /translation="MTGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGK
                     EKLEIVGNLAKRMVKKSGLPIEVHLTLDRREALKDADFVTTQLRVGLLEARAKDEAIP
                     LKYDVIGQETNGPGGLFKALRTIPVILDICKDMEELCPNAWLINFANPAGMVTEAVLR
                     YTNIQRVVGLCNVPIGIRMGLARLLEVDASRVHVDFAGLNHMVYGLDVYLDGVSVMDR
                     VLELVTDPEKQITMENIAALNWEPDFIRGLRAIPCPYHRYYYKTREMLEEEKEASVEK
                     GTRAEVVKQLENDLFELYKDPNLDIKPPQLEKRGGAYYSDAACSLITSIYNNKGDIQP
                     VNTRNNGTIASLPHDSAVEVNCIITKEGPKPIAVGDLPVPVRGLVQQIKSFERTTIEA
                     AVTGDYHKALLAMTINPLVPSDKVAKQILDEMLEAHKEYLPQFFKKVEK"
     gene            complement(6885..7208)
                     /locus_tag="HD73_RS27975"
                     /old_locus_tag="HD73_5612"
     CDS             complement(6885..7208)
                     /locus_tag="HD73_RS27975"
                     /old_locus_tag="HD73_5612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989046.1"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="WP_000989059.1"
                     /translation="MMTTAEQIPFQLILNSGNARSFAMEALQFAKQGKMVEADEAMVK
                     AKEAINEAHHFQTELIQSEARGEKTEVSVLLIHAQDHLMNAITVKELAAEFIDLYKKL
                     EAKGE"
     gene            complement(7390..8697)
                     /gene="celB"
                     /locus_tag="HD73_RS27980"
                     /old_locus_tag="HD73_5613"
     CDS             complement(7390..8697)
                     /gene="celB"
                     /locus_tag="HD73_RS27980"
                     /old_locus_tag="HD73_5613"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168817.1"
                     /GO_function="GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA]; GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="WP_001168848.1"
                     /translation="MQKFIAFMEKYLVPVAGRIGSQRHLVAIRDGFIAVMPLILVGSF
                     AVLINNLPIDAFQNFMKGTFGDAWKDVGGGMWTGSFAILALIVAVGISYNLAKSYGVD
                     ALSAAIITFGALIIISPTTPKEGGIDLAWTGAQGLFVSIIVALLVTEVFRFFVQRDIT
                     IKMPDGVPPAVMKSFAALVPAFVILIVTAGIQLAVKLAGTSIHKMVFETIQLPLQGLA
                     GTLPSAIIIALLIHVLWFFGLHGPNIIGGIIDPIYLPALEKNMKLFNDGMSAYDVPHI
                     FTKPFFDVYVYIGGSGATLAFLVAVMLVAKSAQLRGVGRVSIGPGFFNINEPVIFGTP
                     IVLNPTLIIPFVLTPVVLVITSYTAITLGWVPKTVALVPWTMPPIFSGYLVSGGSIAG
                     AALQVVNFAIAVVIYYPFVLMCDRTYVQAAKAETKGSDNSVSL"
     gene            complement(8714..9016)
                     /locus_tag="HD73_RS27985"
                     /old_locus_tag="HD73_5614"
     CDS             complement(8714..9016)
                     /locus_tag="HD73_RS27985"
                     /old_locus_tag="HD73_5614"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002089627.1"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_001023567.1"
                     /translation="MNILLCCSAGMSTSLLVTKMEAAAKARGLEGKIWAVSGDAVKTN
                     IDQADVLLLGPQVRYMLSSMKTLADEKNVGIDVINPMHYGMMNGEAVLDHALTLKK"
     gene            complement(9363..9680)
                     /locus_tag="HD73_RS27990"
                     /old_locus_tag="HD73_5615"
     CDS             complement(9363..9680)
                     /locus_tag="HD73_RS27990"
                     /old_locus_tag="HD73_5615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068354.1"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="WP_000379645.1"
                     /translation="MDTIETKAFHLILHGGNARSCSMEAIDCAKRGEFTEAEAKLQEA
                     LEELKEAHRVQTELIQKEAGGEKTEVTLLMVHAQDHLMNAITVKELASEFVELYRKMS
                     VDE"
ORIGIN      
        1 atgaaacgaa tagatctcat ttttaacgca atacaaaaag gcaattatac agaaggtgta
       61 accgcatcag aactcgctac atttctgcaa ctagatcgcg ccaatgtaag tagcgattta
      121 aacaaacttg taaaagataa acgtttatta aaaacaaata cgcgacctgt tcgtttttac
      181 atagaaacga acacttcgtt ggaaacgcat tcaaaagaca taacttcatt agatacattt
      241 gcaattgaaa atacgagcct taaaattgca attgaaaaag cgaaagcttc tattctctat
      301 cctccaaacg gtatgcatac tttactactg ggagaaacag gggtcggaaa gtctatgttt
      361 gcttctttaa tgcacgaata tgcaattgaa gttgaacagc ttccaaaaga tgcaccattt
      421 atcgtattta actgtgctga ttatgcaaat aatccacagt tactactcgg gcaattattt
      481 gggattaaaa aaggagctta tacaggagca agtgatcaaa aagggttaat tgaaaaagca
      541 catgaaggaa ttcttttctt agatgaagta catcgcctgc ctccagaggg acaagaaatg
      601 cttttcacct ttattgaccg cggagtatac cgccgccttg gagaaacaga gaacgaacgt
      661 aaagcacaag tattaatcat tactgcaaca acagaagaac caaattcatt tttattaaaa
      721 acatttacaa gacgtatacc aatgactgtt acgctgccac cacttcgtga gcgtacacat
      781 aaagagcgct ttgctttact acagcgattt ttcacaaatg aagcaattcg tctgcgaaaa
      841 gacattcacg ttagcccaaa tgcaatgcgt gctttcgtct tttacaattg tcccaataac
      901 attggtcaat taaaaacaga tgttcaaatt gcctgtgcga aagcctattc tgatttggtc
      961 acaaagaaac gtgattctgt ctatgtttca agttcagatt taccttggta tatgaaagaa
     1021 gggttgttca ttgaaaggaa gagccgtcac ttataccaaa taccaaatga aacatttgta
     1081 tttacaggtg aggaaggttg gagtaatcat aaaacagaag atgaaaaacg ctcttctatt
     1141 tacgattata ttgattataa atacgaagaa cttcaagcac gtggtattga agaggaagaa
     1201 ttagaattac taatagaaaa tgatgttcaa agctttttcg tacaatattt taaccaaata
     1261 tcaaaaaaaa caagccatga aaatgttttt aaaattgtgg atcgtaatat cgtttccgta
     1321 tgtgaaaaaa tagcggaact tgctgaaaag catttatcta agacgtttga tgaaaaagta
     1381 tttctcgctt taagtttaca tgtacagaca actctacaac gtttacaaag cggcaaacaa
     1441 atacatcacc cacaattaaa tcaaattcgt acgaaatata aagaagcttt ttccgtagct
     1501 atgcaatgca ttcaactact ggaggaagaa ttacaaataa caatgcctat tgatgaagct
     1561 ggctttttaa caatgttctt cgttgttgat ccaattcctg cctcacaaac agaagtaaaa
     1621 gtattaatat tagcacacgg taatggcatc gcaactgaaa tggcaaacgt cgcaaacgaa
     1681 cttctcggca ttgaggaagt aaccggtatt gatatgccgt tacacgaatc accgaaagat
     1741 ttcttggaac gtgtgaaagt gtatatgaaa acactacaaa atgtaaatgg gcttctctta
     1801 cttgtcgata tggggtcact tgcttatatc ggtgacatat tagaaacgga gttcaaaatc
     1861 cctgtacgtg ttctctcgat gacgagcaca ccacacgcac tagaagcagc tcgaaaagct
     1921 cagctcggtt attcattaga tgcgctgtat gaaacagtaa aaaatttaac accattttac
     1981 ttaaacgtac aagaagaaaa gaaaaaacct ctctctccaa tgaaatctgt tattttaaca
     2041 gcttgcttaa ctggtgaagg aagtgcttta gctattcaaa aaatgttaga gaactattta
     2101 cgatttgata aagacttaat tgaaattatt ccaattagta tcgtccatga aaaagattta
     2161 acgaaaatga ttgagaacat aaaaaaagag cgtaacatca tttgtattgt cacgaatttc
     2221 gatgtgcaag taccatgctt aacatatcat ttccaagaca tcgtaaacta cacagcgatt
     2281 cagccaattc aagaattaat tacgtatgaa gaaacatatg caaaaatggc ggatattctt
     2341 gaacaacaaa tgcagcgtaa cgatggagca ttacttatca aaacaattcg ttacgcatta
     2401 aatacaattc aggaattaat ttccttacag ctaactcctg atagcttaat gggtgttatt
     2461 ctgcatatga gctgtatggt tgaccgtctt caaaaaggag aaagccttct tcctcaccct
     2521 gataaagaga aaagaagaca agatgagtac tggatgtata tgaaagtaaa aaaagcgttg
     2581 caaccaattg aaaacacatt tgaaatacaa atacctgatg acgaagtatt ttatgttatg
     2641 gacttcttta ttaaaaatca gcctgtgaaa aattaatcga ggccgttttt aaaaatatcg
     2701 atcgtaacgt gagttatatc agcgattttc aaaatatatc ggcgcaaccc agattatatc
     2761 agcgattttc aaaatatatc ggcgtaactc agattatatc agcgattttc aaaatatatc
     2821 ggcgtaactc agattatatc agcgattttc aaaatatatc ggcgtaactc agattatatc
     2881 agcgattttc aaaatatatc gatttaccga caataattgc taaaactagc accttatata
     2941 atgagaaaag gctgttccaa catatggaca gcctttgcct ctatttcttt acttttctaa
     3001 ctcgccctct tatttctcct cttttttgtg atttcgtatg gacattaaca tatacatttg
     3061 cttgaataat ttcttgaagt aaatgtacga atgatttccc ttgtaaaggt ccctcaaaat
     3121 cctctacatt cactacacca gtaatacttc cctcgttcaa gctaatccct tgctccagtg
     3181 gaccatacaa atataaaaca acaggaccaa tttgagtaga cttccctaaa tgaatttgac
     3241 aagatgtaac cttttctata ttttttagta ttactctgta atgcaatttc gttaaatcat
     3301 cactaaaaat aaactctgct accccaaaag cttctgtatt tacaggaggc acttccctct
     3361 tacctgttaa cctggcaaag aaatgtgttg tcataaggca ttctccttat ataaatttcc
     3421 cagcactact tctactactt tatggtttaa acataatcat ttatgacttt ttcaatttta
     3481 attacaaaga aaagattcac aaaatcttta cattgtgatt gttcctgtag attgaaagtc
     3541 tcttgtataa tgcacataga aaaacattat aggaggttcc attgaaaact aacaaataca
     3601 cttttcatac attattagag atttttctcg tctcatttaa attaggactt acttcattcg
     3661 gcggtcctgt cgctcatctc ggctatttcc accacgaata cgtgcaaaaa agaaaatgga
     3721 tggatgaacg aagctatgga gatttagtag cactatgtca attccttccc ggtcctgcca
     3781 gcagtcaagt cggtatgggg gttggattat tacggggcgg tctattagga gctattattt
     3841 catggattgg attcacttta ccgtctgtgc ttgttttggt tttcttcgcc tcattcctta
     3901 atcagttcga tcttggaagt gctggctgga ttcatggact aaaacttgta gcagtcgcta
     3961 ttgtcgctca tgccatatgg ggaatggcgc gaaagttaac tccagatcga aatcgtgcaa
     4021 cgattgcgat tgtaactgcc gcaatcgcct tattatggcc aagtagttgg acacaagtca
     4081 tccttattat aatatgtggc tttatcggtt ggtttttata tcgcaaccaa ccaattagcc
     4141 aatctcaaaa tataagagta cctatttcaa aaaagatagc agtttcttgc ctcgtcttat
     4201 tctttggact attactactg ctaccaatat taagaccgtt ctcttattac atcgctttat
     4261 ttgacagttt ctatcgctct ggcgcacttg tatttggagg aggacacgtc gtgctgcccc
     4321 ttcttgaagg tgagttcgta caaaacggaa tgatgacgaa agaacagttc ctagctggat
     4381 acggattaac acaagcagta ccaggaccac tatttacatt cgcctcttat ataggagcag
     4441 tgttaaacgg gacgcttggg gcaataatcg caacaattgc gattttcctc cctgctttct
     4501 tactcgttat tggtgtttta ccattttgga acaatgtgag aaaaatatca ttcatacaag
     4561 gcgcactact tggtgttaac gccgcagttg ttggtatttt aattgccgct ttttacgatc
     4621 ccatttggac gagtacaatt ataaatgctt cagatttcgt tttcgcttcg cttctttttt
     4681 gcttactagc tttttggaaa gcaccgcctt gggttatcgt tatacttgga gcttttggcg
     4741 gatatattct atccattttg taaataaaaa acgacggcat gatgtagccg tcgtttttct
     4801 attaaaactt cacaagttct atcccttcag gcaactcact ctctgttaaa atacgcaatt
     4861 ccttcacacg atccattacg taagaagaac gcttcacaag ctctggatca atataagctg
     4921 gatgaaccat aatttctacc gtttgttcac cttgtactct ttcttttaac ttcataaagt
     4981 aatcttccgt cacaccatcc gcataaaagt cactgtaaaa tacatcagaa aatggatgca
     5041 ccgctctatc ttccccacaa cgacgaattg gaacattata tttagctgct aatctctcaa
     5101 gaacatcgtg taagatcggt aatccgtgta catgatgatg actatctaaa tgagttggtg
     5161 ttaatccgta agataggaat ttctcaattt gagcagtcca ctctctctca acttcttctg
     5221 gatttatatt cccttccctt acaacacttt gtttatgaaa cgatccgtcg ctacctacaa
     5281 gtgatggtac gtctccaaga agtggctccc ctgctgttaa tacgagatgt acgcctactc
     5341 cgagtaggtt atactctttc gctaagcgta ccgcatgctc tgttcctggc atattcatca
     5401 tcatcgtcgt tgaatttaca agtccattta tatgaccatc aataatgccg taatttgtac
     5461 cttctgtaag accgaaatca tctgcattta caattaactt aatcatcata atacctccta
     5521 atagaataaa aaaagcgggc taaatagcgc cgcttttact tctctacctt tttaaagaac
     5581 tgtggaagat actctttatg tgcttccaac atttcatcta aaatttgttt tgcaacttta
     5641 tctgatggta caagcggatt gattgtcata gcaagcagcg ccttatgata atcccctgta
     5701 acagcagctt caattgttgt gcgctcgaat gatttaattt gttgtactaa accgcgaact
     5761 ggtactggaa gatctccaac tgcaattggt tttgggcctt ctttcgtaat aatacagttc
     5821 acttcaacag ctgaatcatg tggtaagctt gcaattgttc cgttgtttcg tgtattaaca
     5881 ggctggatat cacctttatt attgtaaata gacgtaatta agctacatgc tgcatcacta
     5941 taataagctc cgccacgttt ttctaattgt ggtggtttaa tatctaaatt cgggtcttta
     6001 tataactcaa ataaatcatt ttctaattgt tttactactt ctgcacgtgt acctttttca
     6061 acagaagctt ctttttcttc ttctaacatt tcacgtgttt tgtagtagta acgatggtat
     6121 ggacatggaa tcgcacgaag tcctcgaata aagtctggct cccagttaag cgctgcgata
     6181 ttttccatcg taatttgttt ttctggatct gtcacaagct ctaacacacg gtccattaca
     6241 cttacgccat ctaagtatac atctagtccg tataccatat gatttaaacc tgcgaaatca
     6301 acgtgaacac gactcgcatc tacttcaagt aatcttgcaa gacccatacg aattccgatt
     6361 ggaacgttac atagaccaac tactctttga atatttgtat aacgaagaac tgcctctgtt
     6421 accataccag ctggatttgc aaagttaatt aaccatgcat tcggacagag ctcctccata
     6481 tctttacaaa tatctaaaat aacaggaatc gttctcaacg ctttaaataa accaccagga
     6541 ccatttgttt cctgaccgat tacatcatat tttaatggga ttgcttcatc ttttgcacgt
     6601 gcttctaata aaccaacgcg aagttgtgtt gttacgaagt ctgcatcttt taatgcctca
     6661 cggcgatcaa gcgttaaatg tacctcgatt ggtaaacctg attttttcac catacgtttc
     6721 gctaagttac caactatttc caacttttct tttcctgcct caatatctac taaccaaatt
     6781 tcacgaacag gaagctcatc ataacgctta ataaatcctt caattaactc tggtgtataa
     6841 ctagatccac cgccgattgt agcaatttta attccagtca tgctttattc ccctttcgct
     6901 tcaagctttt tataaagatc gataaactct gctgctaatt ctttcactgt aatcgcattc
     6961 attaaatgat cttgtgcgtg aattaaaaga acactaacct ctgttttttc tcctcttgct
     7021 tctgattgta tgagctctgt ttggaaatga tgcgcttcat taatcgcttc tttcgccttt
     7081 accatcgctt catctgcttc taccattttc ccctgtttgg caaattgaag tgcctccatc
     7141 gcaaagcttc gtgcgttacc actatttaaa atcaattgga atggaatttg ttctgctgta
     7201 gtcatcataa ttaccgccct ctctataagg ttcaaccgat ttaaataccc cttcagtttg
     7261 aacatgtcta ttaaattatt attttatgaa tgatagtttg gtgttcttgt agatgagtat
     7321 catctttttg aacatgatat aaaggtaatt attctgacga gctataggaa ctcgccagaa
     7381 taatattttt taaagtgata ctgaattgtc gcttcctttt gtttcagctt ttgcagcctg
     7441 tacataagta cggtcacaca ttaatacgaa tggatagtaa attactaccg caatagcaaa
     7501 gttcaccact tgtagtgctg caccagcgat gcttccgccc gatacaagat aaccactaaa
     7561 gattggtggc attgtccatg gaactaacgc aactgttttt ggtacccatc ctaaagtgat
     7621 tgctgtataa gaagtaataa ctaatacaac tggtgtaagt acgaacggga taattaacgt
     7681 tgggtttaat acaattggcg taccgaaaat aactggttcg ttaatattaa agaaaccagg
     7741 accgatagat acacggccta caccgcgaag ctgcgcactt ttcgctacaa gcattactgc
     7801 cactaagaat gctaacgttg cacccgatcc cccgatatat acgtatacat caaagaatgg
     7861 ttttgtgaag atatgcggta cgtcatatgc agacatacca tcattaaata atttcatatt
     7921 tttctctaat gctggtaaat agattgggtc aataatcccc ccaataatat ttggaccatg
     7981 taatccgaag aaccataata cgtgaataag tagagcaata ataattgcac ttggtaatgt
     8041 acccgctaaa ccttgtaatg gtaattgaat tgtttcaaat accattttat gaatgcttgt
     8101 tcccgcaagt ttcacagcta attgaatacc tgctgttaca atcaaaataa cgaatgctgg
     8161 aactaacgct gcgaatgatt tcattactgc cggtggaaca ccatctggca ttttaattgt
     8221 aatatccctt tgaacaaaga atctaaacac ttctgttaca agaagagcca ctataatgga
     8281 aacgaataga ccttgtgccc ctgtccacgc taaatcaata ccgccttctt ttggcgttgt
     8341 cggagaaata ataattaatg caccgaatgt aatgattgct gctgacaacg catcgacgcc
     8401 gtaagatttc gctaaattgt aacttatccc aactgcgact atgagggcga gtatagcaaa
     8461 ggaaccagtc cacataccgc caccgacatc tttccaagcg tcaccaaatg tgcccttcat
     8521 aaagttttgg aaagcatcaa ttggtaagtt atttataagt actgcaaatg atccaactaa
     8581 aataagaggc ataactgcga taaatccgtc acggattgca actaagtggc gctgcgatcc
     8641 aattctacct gcgactggaa ctaaatattt ctccataaat gcaataaact tttgcatttc
     8701 gcttcccccc taattatttt ttaagtgtta atgcgtgatc taaaactgct tctccattca
     8761 tcatgccgta gtgcattgga ttgataacgt cgattccaac atttttctcg tcagcaagcg
     8821 ttttcattga agagagcatg taacgaactt gtggtcctag taataacaca tctgcttgat
     8881 cgatattcgt ttttactgca tccccagata cagcccaaat cttgccttct agaccgcgag
     8941 cttttgcagc cgcttccatt tttgtaacta gtaaacttgt agacatccct gctgaacaac
     9001 ataataaaat attcatttga aaatccccct tgtgtagtat aaaaaattta tgtttattta
     9061 tttttcttta cgctatttat taatgcaatt agcgtgccaa ctttttaaaa ccactaaaat
     9121 cagtgctttt tcgagcattt tctttgtgtg ttaacgcttt caattagtgt gtcgtccaaa
     9181 aaatacacag tgtgttataa aacaacacgc ttttaacaca taactgtatg aaaatagatt
     9241 agtgtttagt cgtctagttg tgtttaatat gaaaaaacac taaaaaaact ttttcaatga
     9301 aaaataattc attattttaa cccactattt agtgaaataa aaaaaccttg cataagcaag
     9361 gttcattcat ccaccgacat cttcctatat aactctacaa actcactcgc taattctttc
     9421 accgtaattg cattcattaa atggtcttgc gcgtgtacca ttaaaagagt aacttcggtc
     9481 ttttcgccac cagcctcttt ctggataagc tccgtttgta cgcggtgcgc ctcctttaat
     9541 tcctctagtg cttcttgtaa tttagcttca gcttctgtaa actccccacg cttcgcacaa
     9601 tcaattgctt ccattgaaca acttctcgcg tttcccccat gcaagattaa atgaaaagct
     9661 ttcgtctcta tcgtatccat
//
