LOCUS       NC_012034               6571 bp    DNA     linear   CON 13-DEC-2024
DEFINITION  Caldicellulosiruptor bescii DSM 6725, complete sequence.
ACCESSION   NC_012034 REGION: 697213..703783
VERSION     NC_012034.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02598462
            Assembly: GCF_000022325.1
KEYWORDS    RefSeq.
SOURCE      Caldicellulosiruptor bescii DSM 6725
  ORGANISM  Caldicellulosiruptor bescii DSM 6725
            Bacteria; Bacillati; Bacillota; Bacillota incertae sedis;
            Caldicellulosiruptorales; Caldicellulosiruptoraceae;
            Caldicellulosiruptor.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meincke,L., Brettin,T.,
            Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Ovchinnikova,G., Kataeva,I. and Adams,M.W.W.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of chromosome of Caldicellulosiruptor becscii DSM
            6725
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meincke,L., Brettin,T.,
            Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Ovchinnikova,G., Kataeva,I. and Adams,M.W.W.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2009) US DOE Joint Genome Institute, 2800
            Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP001393.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4084710
            Source DNA available from Michael Adams (adams@bmb.uga.edu)
            Bacteria available from DSMZ: DSM 6725
            Contacts: Michael Adams (adams@bmb.uga.edu)
                 David Bruce (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Meta information:
             Organism display name: Caldicelulosiruptor becscii Z-1320, DSM
            6725
             Culture Collection IDs: DSM 6725
             GOLD ID: Gi03121 http://genomesonline.org/GOLD_CARDS/Gi03121.html
             Sequencing Platforms: Sanger, 454
             Phenotypes: Thermoacidophile, Cellulose degrader
             Diseases: None
             Habitat: Fresh water, Hot spring
             Oxygen Requirement: Anaerobe
             Temperature Range: Thermophile
             Biotic Relationship: Free living
             Isolation: Hot spring on the Kamchatka peninsula in Russia.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000022325.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 12:57:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,844
            CDSs (total)                      :: 2,783
            Genes (coding)                    :: 2,660
            CDSs (with protein)               :: 2,660
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 71 of 123
            Pseudo Genes (incomplete)         :: 66 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 46 of 123
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Caldicellulosiruptor bescii DSM 6725"
                     /mol_type="genomic DNA"
                     /strain="DSM 6725"
                     /type_material="type strain of Caldicellulosiruptor
                     bescii"
                     /db_xref="taxon:521460"
                     /note="type strain of Caldicelulosiruptor becscii DSM
                     6725"
     gene            1..1308
                     /locus_tag="ATHE_RS03060"
                     /old_locus_tag="Athe_0614"
                     /db_xref="GeneID:31771969"
     CDS             1..1308
                     /locus_tag="ATHE_RS03060"
                     /old_locus_tag="Athe_0614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="WP_015907191.1"
                     /db_xref="GeneID:31771969"
                     /translation="MKRLGKIVALVTLVVFMLSLVLVFSPSEKAFSSAGGTKKIVFWH
                     ITTDAVGKQMIQNQVNRFLKAHPDFKVEVVPLQNDSFKTKLKIAMGSNQAPDVFVTWG
                     GGPFFEYVRAGKVKDITAYMNEKNYKSRFVDAAFGPITYNNKIWAVPVEGAGVALVWY
                     NKEIFKKYNLKVPTTYDEFLNIVKVLKSKGITPITLANKTKWPGCFWYWNLVNKLGGA
                     SAFEKAAERTGGSFADEPFVKAGQMLQDLVKMGAFPKGFNGLDWDTGQSRMLLYSGKA
                     AMELMGTWQIPIVKAEKKEFYEKNLDFFPFPSIKGGKGDPTSLVGMASSNYYAVTTTC
                     KYPKEAFNMIQYLIDDQAVKERINYGQIPPVKGLKFSDPMLQKVYNIVAKAKSIQLWW
                     DQYLPPQLAETHKDIVQSLFGLTITPKAAAEKMEKAAKEYFKK"
     gene            1440..2330
                     /locus_tag="ATHE_RS03065"
                     /old_locus_tag="Athe_0615"
                     /db_xref="GeneID:31771970"
     CDS             1440..2330
                     /locus_tag="ATHE_RS03065"
                     /old_locus_tag="Athe_0615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430929.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_015907192.1"
                     /db_xref="GeneID:31771970"
                     /translation="MQRDKASKLMQNLGILLFLAPALILIGVYIIWPIIMSFDLSMYE
                     WDGVSPQKLYLGFKNWIDLIHDSIFWHAFGNNIKFIFFSIIIEMPIAILIAVMLDRGG
                     KKFDAFKSLFYLPMLMSSVAIGVLFKYIYDPSFGLLAGILNFLHLDFLNQDWLGDPNV
                     AFYSVVAVICWQYIPFYMIFFIAAISNIPHELYEAARIDGATQGQYFWKIELPLLAPS
                     IKTACILSLIGSLKYFDLIYVMTEGGPSNATELMATYMYKNAFASFKMGYGSTIASAM
                     FIIITTAGILAYFATKKKEA"
     gene            2368..3195
                     /locus_tag="ATHE_RS03070"
                     /old_locus_tag="Athe_0616"
                     /db_xref="GeneID:31771971"
     CDS             2368..3195
                     /locus_tag="ATHE_RS03070"
                     /old_locus_tag="Athe_0616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042943.1"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_015907193.1"
                     /db_xref="GeneID:31771971"
                     /translation="MGFAKEIKKWYFIFLAIWTLVADVPFLFMFFTSIKTQSELLEGN
                     TWQIPKQPTVGNYSTVIHGSFFNYLKNSVIAVSISVILILIISSMAAYVFARMKFSLN
                     NILYSLIIAGMAIPIHVTLIPIYVLTNKIKLYDTIFALIGPYVALSLPMSIFILTEFM
                     REIPIELEEAAKIDGCSMFRLYSDILLPLSRPALITVGIYNGTYLWNEFVFALVLTSS
                     PDKRTLPLGIWDFQARYGSDIPAIMAFLTLSLLPMLIAYILGQDKIIKGMMAGAVKG"
     gene            3288..4487
                     /locus_tag="ATHE_RS03075"
                     /old_locus_tag="Athe_0617"
                     /db_xref="GeneID:31771972"
     CDS             3288..4487
                     /locus_tag="ATHE_RS03075"
                     /old_locus_tag="Athe_0617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="WP_015907194.1"
                     /db_xref="GeneID:31771972"
                     /translation="MGNHTLLKQINKLLVLKTILDNKIISRTKISKLVDLNKATVSNL
                     TDELIKEGYVVEKGYGKSKGGRRPVLLQVNKDVGSIIGIDLGVDYIHIILSNFVGEVI
                     FEEYANMKIGEDKEKLLRLLFDLIEKSVKKAPQTPKGILGIGIGVPGIIEKESGTVLL
                     APNLKWQNVPLRSIVQQKFNLPVYIDNEANAGALGEKWFGEWGKVSDLIYLSVGIGLG
                     AGIIIDNKLFRGAAGFAGEVGHTTINFQDDVCSCGNIGCLENFASERALLSVIKKLVK
                     QGVEDRYISWENVDEITPSRIIQAAKEGSRVCRMAILEVAEKMGIGVANLVNIFNPEM
                     VIIGNKASFFGELFLEKLREVINQRSFIAQFYNLKIEVSKLKDRAVVLGCIAMVISDM
                     LSFPEYA"
     gene            4502..6571
                     /locus_tag="ATHE_RS03080"
                     /old_locus_tag="Athe_0618"
                     /db_xref="GeneID:31771973"
     CDS             4502..6571
                     /locus_tag="ATHE_RS03080"
                     /old_locus_tag="Athe_0618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907195.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="WP_015907195.1"
                     /db_xref="GeneID:31771973"
                     /translation="MSVCLYKRLKRFSVLILVILFLIVTLIGIETTKTSRVIGETKKS
                     FVEYNFENRFIAPFKASGKSMSLRIDNTTAAEGTFSLLASGRKQIDDGILLDVTNLID
                     YANEYKITLYVYHKSSKMQRFVVSSEIETKSGKENKLLCEKIIIPKSWKKLDANLNLT
                     EQKGIKKVWLKVYVPTSTTNFYVDLFTLKVADKSHLIKFENFEDKSIAGFISQDKKCK
                     LSISKEKAYQGTYSIKVQQTVKRQNTTVILPVKGTFEKGKSYSISFYMHQSILKSLNF
                     AVGIRFLESGKNMREIVLGRVTIPKNKWTEVFASYTPSLDSKIKDFVIFIRPLSDISY
                     YYIDNFTISDDGWYSAVPDLDLPSLREKYKNYFKIGVAVPYRALTNPVDVAFIKRHFN
                     SITAENEMKPEALEPIEDNFNFSIADEYLNFCRKNNIAIRGHTLVWHQQTPNWFFENP
                     QTGKKLTNSEKDKKILLERLKKYIQTVVSRYKGRIYAWDVVNEAIDENEPDGFRRSDW
                     FNVLGPEYIEKAFIYAHQADPNAQLFYNDYSTENPVKREYIYKLIKSLREKGIPIHGV
                     GLQCHISVSWPSVEEVEKTIKLFSSIPGIKIHVTEIDISVAKEYGEDIDEETKRYLLI
                     QQARKLKDLFDVFKKYKNVVTSVSFWGLKDDYSWLQGDFPLLFDKDYQPKFAFWSLID
                     PSVVPEE"
ORIGIN      
        1 gtgaagaggc ttggaaaaat tgtagcgttg gtaactttgg ttgtttttat gttgagctta
       61 gttttagttt tttcaccatc tgaaaaggct ttcagctcag ctggtggaac aaagaagatt
      121 gtattttggc acatcacaac tgatgctgtc ggaaagcaaa tgattcaaaa ccaagtaaac
      181 agatttttga aagcgcatcc agactttaag gttgaagtag ttccgctaca gaatgattca
      241 ttcaagacaa aattgaaaat tgctatgggt tctaaccagg caccagatgt atttgttaca
      301 tggggtggcg gtcctttctt tgaatatgta agggctggta aggtaaaaga tattacagct
      361 tatatgaacg agaaaaatta taagagcaga tttgttgatg cagcatttgg gccaattact
      421 tataacaata agatatgggc agttccagtt gaaggcgctg gtgttgcgct tgtatggtac
      481 aataaggaaa tattcaaaaa atacaatttg aaagtaccaa cgacttatga tgagtttcta
      541 aatattgtaa aggtattaaa atcaaaagga ataactccaa taactcttgc taataaaaca
      601 aaatggcctg gttgtttctg gtactggaat ttggtaaata aattaggtgg tgcttcagcg
      661 tttgaaaaag ctgctgagag aacaggtggt agctttgcag atgaaccgtt tgtgaaagca
      721 ggtcaaatgc ttcaggattt ggtcaaaatg ggggcattcc caaaaggatt taatggactt
      781 gactgggata cgggacaatc aagaatgctt ttatactctg gtaaagctgc aatggaatta
      841 atgggaactt ggcagatacc tatagtaaag gctgaaaaga aagaatttta tgaaaagaat
      901 cttgatttct tcccattccc atcgattaag ggtgggaaag gagatccaac aagtttagtt
      961 gggatggcgt cttcaaacta ctatgctgtg acaactacat gtaagtatcc aaaagaagca
     1021 ttcaatatga ttcaatactt gattgatgat caggctgtaa aagagagaat taactatgga
     1081 caaattcctc ctgtaaaagg attaaagttt tctgatccaa tgcttcaaaa ggtttacaac
     1141 atagttgcaa aagcaaagag tattcagctt tggtgggatc aataccttcc accacaactt
     1201 gcagagactc ataaggatat tgtgcaatct ctctttggct tgacaattac accaaaagct
     1261 gctgcagaaa aaatggagaa agctgctaag gaatatttca agaagtaaga attaaatatt
     1321 ttatactgat aaagaaaatt ggtattagcc atggaaagta aatctccatg gctaatacca
     1381 attcaatttt ctataaaact gtttaaatta aaacttaatt ttggggagtg aagttaaagt
     1441 tgcagagaga taaggcatca aaactaatgc aaaatttagg cattttattg tttttggctc
     1501 ctgctttaat tcttattgga gtttatataa tttggccaat aattatgtct tttgacctca
     1561 gtatgtacga atgggacggt gtttctcctc aaaaattata tcttggtttt aaaaactgga
     1621 ttgatcttat acatgactcg atattttggc acgcatttgg taataatatt aaatttatat
     1681 ttttttctat aataattgaa atgccaattg caatattgat agcagtaatg cttgatagag
     1741 gtggcaaaaa gtttgatgca tttaagtcac tgttttatct tccgatgtta atgtcatcag
     1801 tggcgattgg tgtactattt aaatatatat atgatccatc atttgggctt ttagctggaa
     1861 tacttaactt tttacacctt gactttttaa atcaagattg gctgggtgat ccaaatgtag
     1921 ctttctactc tgttgttgct gttatatgtt ggcaatatat acctttttat atgatattct
     1981 ttatagctgc aatttctaac attcctcatg aactttacga agcagcaaga atagacgggg
     2041 caacacaagg tcagtatttt tggaaaatag aacttcctct tttagctccg tccataaaga
     2101 ctgcttgtat tttatccttg attggttctc ttaaatattt tgatttgatt tatgttatga
     2161 cagagggcgg accgtcaaac gcaacagaac tcatggcaac ttatatgtat aaaaacgctt
     2221 ttgcctcatt taagatggga tatggcagca caattgcttc ggcaatgttc ataataatta
     2281 caacagcagg aattttagca tattttgcga caaagaaaaa agaagcttaa attttaagtt
     2341 tgcgccaaaa ggaggtacaa tactataatg ggttttgcaa aagaaattaa aaaatggtat
     2401 ttcatatttt tagctatatg gacattagtt gcagatgtgc catttttgtt tatgtttttt
     2461 acgtctataa aaacacaatc agagctactt gaaggtaaca catggcaaat accaaaacag
     2521 cctactgttg gaaactattc aactgtaatt catggtagtt tttttaacta cctcaaaaat
     2581 agtgttattg cagtttcaat ttcagtaatt ttaatactta ttatatcttc aatggcagcg
     2641 tatgtatttg cgagaatgaa attttctctg aataatatat tgtattctct tataattgca
     2701 ggaatggcaa ttcctattca tgttacttta atcccaattt atgttcttac taataaaatt
     2761 aagttatatg atactatatt tgcattaatt gggccgtatg tagcattaag tttgcctatg
     2821 tcaatattta tcctgacaga gtttatgcga gagattccta ttgagcttga agaagcagct
     2881 aaaatagatg gatgttccat gtttagattg tattctgata tccttcttcc tctttcgaga
     2941 cctgctttaa ttacagttgg catatacaat gggacatacc tttggaatga gtttgttttt
     3001 gctttagtat taacaagttc gccagataaa aggacactcc cacttggcat ttgggatttt
     3061 caggcgaggt atggttcaga cataccagct atcatggcat tcttaacatt atcgcttttg
     3121 ccaatgttga ttgcgtatat tttgggtcag gacaaaatta taaaaggtat gatggcaggt
     3181 gcagtaaaag gataagaagt tgttttagcg tcaaaaactg ttttcccgca acctattttt
     3241 gttgtataat aatttgcaaa gcttaattag caaaggaaaa tgacaggatg ggtaaccaca
     3301 cgctactaaa gcaaataaac aagcttttgg ttttgaaaac aattttggac aacaagataa
     3361 tatctcgtac aaagatatcg aaacttgtag atttaaataa agcaacagtc tcaaacctca
     3421 ccgatgagct cataaaagaa gggtatgtag tagaaaaagg atacggtaag tccaaaggcg
     3481 gaagaagacc tgtcctttta caggtaaaca aagatgtagg ttcaatcatc ggaattgact
     3541 taggtgttga ctatattcat attattctct caaactttgt gggagaagtt atttttgaag
     3601 aatatgccaa catgaaaata ggtgaggata aggaaaaact tttaaggctt ctttttgacc
     3661 tgattgaaaa atcagtaaaa aaggcgccac aaactccaaa agggatttta ggtattggaa
     3721 ttggtgttcc aggtattata gaaaaagagt ctggaactgt ccttcttgct cccaatttga
     3781 aatggcaaaa tgtccctttg aggtcaattg ttcagcaaaa gttcaacctc cctgtttata
     3841 ttgacaatga agcaaatgca ggcgcactgg gcgaaaagtg gtttggtgag tggggaaaag
     3901 ttagtgattt gatttatttg agtgttggaa ttgggcttgg tgcaggaatt attatcgaca
     3961 acaaactttt cagaggtgct gcaggatttg caggtgaagt tggacatacc actatcaact
     4021 ttcaggacga tgtttgcagc tgcggcaata ttggctgtct tgagaacttt gcatccgaga
     4081 gggcactttt gagtgttata aaaaagcttg taaaacaagg agtagaggat aggtatataa
     4141 gctgggaaaa tgtagatgaa atcactcctt ctcggataat acaagcagca aaagaaggaa
     4201 gcagagtttg cagaatggct atacttgagg ttgctgaaaa gatggggata ggcgtggcaa
     4261 atcttgtaaa tatttttaat cctgaaatgg taattatagg taataaggca tcattttttg
     4321 gagagttgtt tttagaaaaa ttgagggaag taattaacca gagatccttt atcgcccagt
     4381 tttataatct taagatagag gtttcaaaac tgaaagacag agctgtggtg ctgggatgta
     4441 tagctatggt gatttccgac atgctttctt ttccagaata tgcatgagga ggaggtacta
     4501 agtgagtgtt tgtctttata aaaggttgaa aaggttttca gtattgattt tagtaatttt
     4561 atttttgatt gtaaccttaa ttggtataga aactactaaa actagtagag tcattggaga
     4621 aacaaaaaag agttttgtgg agtataattt tgaaaatagg tttatagcac catttaaagc
     4681 gtcaggaaaa tctatgagtt taaggattga caatacaaca gcagcagaag ggaccttttc
     4741 acttcttgca agtggccgaa agcaaataga tgatggaatt ttgcttgatg taaccaatct
     4801 tatagattat gcaaatgaat ataaaattac tctatatgtt tatcacaagt caagcaaaat
     4861 gcagcgtttt gttgtgagta gcgaaattga gacaaaaagc ggaaaagaaa acaaactgct
     4921 gtgtgaaaaa attattattc caaaaagttg gaaaaaactt gatgcaaatt taaatttgac
     4981 tgagcaaaaa gggataaaaa aagtttggct aaaggtatat gtgccaacct caaccacaaa
     5041 tttttacgtt gaccttttta cactcaaagt tgctgacaaa tctcatctaa taaaatttga
     5101 aaactttgaa gataaaagta tagcaggatt tatctctcaa gataaaaaat gtaaattatc
     5161 catttcaaaa gaaaaagctt accaaggtac atatagcata aaagttcagc aaacagttaa
     5221 aagacaaaac acaacagtta tcttgcctgt aaaaggaaca tttgaaaagg ggaaaagtta
     5281 ttcaatatct ttttatatgc atcaatccat tctaaagagt ttaaattttg cagttggtat
     5341 aaggtttctg gaaagtggaa agaatatgag agagattgtg cttgggaggg taaccattcc
     5401 aaagaataag tggacagaag tatttgcaag ttatacgccc tctttggatt caaagataaa
     5461 agactttgtg attttcataa gacccctctc agatatttcc tattactata tcgacaactt
     5521 tacaatttca gatgatggat ggtattcagc tgtacctgat ttagatttac cttctttgag
     5581 agagaaatac aaaaactact ttaaaattgg tgttgctgta ccatacaggg ctttgacaaa
     5641 tccagttgat gttgctttca taaagagaca ttttaacagc attacagcag agaatgagat
     5701 gaagccagaa gcacttgaac caatagagga caatttcaac ttttcaattg ctgatgagta
     5761 tcttaatttc tgcagaaaga ataatatagc tattcgtggt cacacacttg tgtggcatca
     5821 gcaaacccca aactggtttt ttgaaaaccc tcagacaggg aaaaagctga cgaatagtga
     5881 aaaagacaaa aagatacttt tagagaggct aaaaaagtat atccagacag ttgtttcaag
     5941 atacaagggg agaatatatg catgggatgt ggtaaatgaa gctattgatg agaatgagcc
     6001 agatggattt agaagaagtg actggtttaa tgtattgggt ccagagtata tcgaaaaagc
     6061 gttcatatac gctcatcagg cagaccctaa cgctcagctt ttttacaatg actacagcac
     6121 agaaaatcca gtaaagagag aatatattta caagctaata aagagtctaa gagaaaaagg
     6181 aatacccatt catggtgttg gtcttcagtg tcatatttct gtaagctggc caagcgttga
     6241 agaggtagaa aaaactataa aactttttag cagcattcct ggtattaaga tacatgtcac
     6301 agaaattgat ataagtgtgg caaaagaata tggtgaagac atagatgaag aaacaaaaag
     6361 atatcttttg attcagcagg caagaaagtt aaaagatctt tttgatgtgt tcaaaaaata
     6421 caaaaatgta gtaacaagtg tttcgttctg gggactcaaa gatgactatt catggcttca
     6481 aggtgatttt ccacttttgt ttgacaaaga ttatcaacca aaatttgctt tctggagctt
     6541 aatagacccg tcagttgtgc cagaagaata a
//
