LOCUS       NZ_CP090616            21550 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Vibrio pelagius strain WXL662 plasmid p_1, complete sequence.
ACCESSION   NZ_CP090616 REGION: 203954..225503
VERSION     NZ_CP090616.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24583363
            Assembly: GCF_024362845.1
KEYWORDS    RefSeq.
SOURCE      Vibrio pelagius (Listonella pelagia)
  ORGANISM  Vibrio pelagius
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Vibrionales; Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 21550)
  AUTHORS   He,X.
  TITLE     Alginate degradation mechanism of Vibrio pelagius WXL662
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21550)
  AUTHORS   He,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2022) marine ecology, ocean university of China,
            yushan, qiangdao 266000, China
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            CP090616.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: canu oct-2019 v. oct-2019
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 100.0x
            Sequencing Technology  :: 100.0
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024362845.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 07:57:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,113
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,891
            CDSs (with protein)               :: 3,891
            Genes (RNA)                       :: 182
            rRNAs                             :: 14, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 138
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 21 of 40
            Pseudo Genes (incomplete)         :: 23 of 40
            Pseudo Genes (internal stop)      :: 7 of 40
            Pseudo Genes (multiple problems)  :: 9 of 40
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21550
                     /organism="Vibrio pelagius"
                     /mol_type="genomic DNA"
                     /strain="WXL662"
                     /isolation_source="surface seawater"
                     /db_xref="taxon:28169"
                     /plasmid="p_1"
                     /geo_loc_name="China: Qingdao offshore"
                     /collection_date="2015-07"
     gene            5..700
                     /locus_tag="LZI70_RS20395"
                     /old_locus_tag="LZI70_20365"
     CDS             5..700
                     /locus_tag="LZI70_RS20395"
                     /old_locus_tag="LZI70_20365"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="WP_255232885.1"
                     /translation="MITLRHANDRGTANFGWLDSKHTFSFGHYYDPKHMGFSALRVIN
                     DDLVKPGAGFDTHGHRDMEIISYVLEGVIEHKDSEGNVQTLPAGEFQLMSAGSGIYHS
                     EYNGSQSEDLKFLQIWIQPNTSGNKPGYQQKDFGKNTGLTAIATPDGRDGTLHIKQDA
                     SLFQLILEPGQSIELPITNGRKVYVHQVTGQLSVDEVLLDAGDGAKVEAQNQVNFANQ
                     SSESVTALVFDLP"
     gene            complement(1023..3059)
                     /locus_tag="LZI70_RS20400"
                     /old_locus_tag="LZI70_20370"
     CDS             complement(1023..3059)
                     /locus_tag="LZI70_RS20400"
                     /old_locus_tag="LZI70_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017028305.1"
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="WP_255232886.1"
                     /translation="MIQFSEKQIQSIKQRASEQTIAQLIEDNRVVLNSDTLVPPDGRA
                     TWNLYYFCPEHGVRLTWDREKPLSHCCPVDGKEFTGEPYDGAWWRWLNGLNAKACYDL
                     GLLWNLTGEQRFFDKVKDILLQSAKYYPDYQVHGGIPYNGPGKANAQTLCEANCHLDL
                     ARGYDFIREQLTSDEQSYIEQRLLREGAEFLMEYRTDQLHNHEMKINATIGVIGLILE
                     ESRYIDFALNTDYGIHYQLNHGCSEEGMWFEGSIHYHYYALQALLNFEKIAYSTPHSV
                     SSNPNFVKMLKFPLNLVLNTGDFPRLNDCIAGQERITHSHLFEFAYAQYPCGEFASVL
                     SSIYQNISRDNIDALLYGVDELPKAAPLQCQSIHTTQAGVTIHYDQSNDHSLLLKHSP
                     YGGEHDHYDRLGLIIVRNGKEILPDLGTTGYGAELHYGYYKNTATHNTLVVNQQNQSP
                     IDPILLHFSDSPICKVIDTLADWNQPPAEVDSHTIEQWDSQAYQNVMFRRILFCVGEA
                     YVETNLIANPHKQQLDLTYHVRGEHTDTDNFENCSNPLPGALNRMTNTRQLSSVSAHS
                     HRYTIEGQTPFSQRMVFSSPCDVLIGEAPDNPATSNLAYTLIRSNATALNTLVLHDLS
                     ESAELLLEDVSWSDTEITFSINQDGNKTTITYNLHSHSLRTRSWHPEHEKLTFS"
     gene            complement(3056..4489)
                     /gene="uxaC"
                     /locus_tag="LZI70_RS20405"
                     /old_locus_tag="LZI70_20375"
     CDS             complement(3056..4489)
                     /gene="uxaC"
                     /locus_tag="LZI70_RS20405"
                     /old_locus_tag="LZI70_20375"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431359.1"
                     /GO_function="GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="WP_255232887.1"
                     /translation="MNHYIHDDYLLTSDLARHLFHDYAKAMPIIDYHNHLDAKQIWEN
                     HRASNIAECWLHSDHYLWRAMRSNGIEEHYITGDASDMEKFAKWCQTAPYLLGNPLYQ
                     WSHLELERFFNCSLILNPDNCDAIWSHCEAELATSRLTTQNVLKQCNVTALCTTDSPL
                     SDLRYHQLIAESDFDVEVLPTFRADDLFAFDSPTAFRNMIDTLSTITALHIASINDFL
                     NAISKRVEAFHDAGCRLSDLGLTQVNFAPCSDNTAQQLFEKLLSGQVLKDTESTQLSS
                     YLFNHLGQHYHSRGWAMQLHIGVLVNVNERRKQELGGGTGFSVMNDRLIAENLTQLLS
                     QLDLTNQLPKTVLYSINPNHNALLSCIAGAFQDSDSAAGKIQFGAAWWFNDHKDGMEA
                     QLTTLKNLGALGRFIGMLTDSRNVFSMSRHEYFRRVLCNQLASWVENGELPNNDALLK
                     ETIENICYYNAERYFNFPQSELTGDAQ"
     gene            complement(4619..6385)
                     /locus_tag="LZI70_RS20410"
                     /old_locus_tag="LZI70_20380"
     CDS             complement(4619..6385)
                     /locus_tag="LZI70_RS20410"
                     /old_locus_tag="LZI70_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017028303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="WP_255232888.1"
                     /translation="MNIDIVVVGVYFVFMIAVGVIFKRLAGNSTSDYFRGGGKMLWWM
                     VGSTAFMTQFSAWTFTGAAGKAFTDGFPIMVIFMANAFGFLLSWLFFSYRFRQMRVVT
                     PVEGVRRRFGTTNEQVFTWATMPTSIVYTGIWLNGLALFVSAVFKIDIETTIVVTGLI
                     VLFISVIGGSWGVVASDVVQMVVIMAVTVVCAVAALIKIGGPSNLIEQFPADSIMGAN
                     MNYPLLFVAWFIFMFVKQLQNINNMQDSYRFLTAKDSTNARKAALLAFVLMLIGPAIW
                     FMPPWVTAVLYPDAALAHADALGGKAADAVYLVFVERVMPVGMVGLLMSAVFAATMSS
                     MDSGLNRNAGVFVRNFYSPIINKKASEKKLMRVSQGVTMVFGALIIAVALFINSLRGL
                     SLFDAMMYVSTLLQMPILVPLFFGMFIKKTPDWAAWATLTVGMVVSYMVSFVITPDVI
                     ANLLGIEGGFTSREASDLTVMNGIIGHLVFTGGFFCLTTKFYKEPQGERKTELNEFWT
                     DVATPVIEEEGQDAVDRQQRSMLGKLILIFGVLVVAMVLIPNPFWGRMAFLFCGGVVL
                     TVGALLLKSAKETKVLKVQTQS"
     gene            6909..8675
                     /locus_tag="LZI70_RS20415"
                     /old_locus_tag="LZI70_20385"
     CDS             6909..8675
                     /locus_tag="LZI70_RS20415"
                     /old_locus_tag="LZI70_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016768627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="WP_255232889.1"
                     /translation="MNVDMLVVGVYFIFMIAIGVIFKRFAGSSTSDYFRGGGKMLWWM
                     VGSTAFMTQFSAWTFTGAAGKAFTDGFPIMVVFMANAFGFLLSWLFFSYRFRQMRVVT
                     PIEGVRRRFGGTNEQVFTWATMPTSIVYTGIWLNGLALFVSAVFKVDIETTIVVTGLI
                     VLFISVIGGAWGVVASDFVQMVVIMAVTVVCAIAALVKIGGPTNLIEQFPADSIMGSD
                     MNYPLLFVAWFIFMFVKQLQNINNMQDSYRFLTAKDSTNARKAALLAFVLMLIGPAIW
                     FLPPWVTAVMYPDAATVHAAELGKKAADAVYLVFVENAMPVGMVGLLMSAVFAATMSS
                     MDSGLNRNSGIFVRNFYAPILDKKADDKKLMRVSQIVTMCFGGLIILVALFINSLRGL
                     SLFDAMMYVSTLLQMPILVPLFFGIFIKKTPDWAAWATLAVGIVVSYMVSFVITADVV
                     ANWLNLDTPFTGREAKDLKVLLGIMGHLFITGGFFCLTTRFYKEPVGERKSELTEFWN
                     DVATPVVEEAGQDELDRQQRNMLGKLILVFGLLVTLMVLIPNPFWGRMAFVFCGAVIV
                     TVGSLLLKSAKQADKLTASSAT"
     gene            8784..10778
                     /locus_tag="LZI70_RS20420"
                     /old_locus_tag="LZI70_20390"
     CDS             8784..10778
                     /locus_tag="LZI70_RS20420"
                     /old_locus_tag="LZI70_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="WP_255233015.1"
                     /translation="MNVNTLAILIPLTLYSAPGLSAPGYKQGATLPTTFPNTIYAVDH
                     GIIADDGIDDSAALQAIIDNEVTASTSNNTLIVLPKGTINLSDEIHIDKSGVVIKGAG
                     SDPDTGTQIVVKSWSPYGVDASNAPDFDKKYWPGFGVFRVETREKHAKEPAYEGSVNF
                     HWKHSIEFAQPAKMGDTKIVLESGKAGEFSQGDLIYVGAATDRAFLDEGQVPESRRSA
                     SHIKTGHMRTQIFKVTEVNTSSNTVTLDGALEFDIPLNNEGGYNSRVMPVRAIENFGL
                     QDFFLTMDIEGSHCEGYNRNQFSESNPNGVLYRYENVCAQDAIHGIILKWAYNGWVDN
                     VRIDMIGSHPIVSEFAKAVTISNNTIDGSWNKGAGGNGYVRGSKLYNSWIHNNDIRNI
                     RHLALQWSATGNIVENNTLNVDMNFHGGWERNNLVRSNHIAVPFEHRSWSNGAPESGA
                     TWQPIWVGSGDHASKWSGPTGPNNVLVNNTLKKAKNSGDSITRWGLFDEPDVEYALNW
                     DGSQYKHLNINGEPIATWSQTLAEGVHQQIPQSGVTVAGGSWPPEGVDPEEPVDPVDP
                     PVSEGCDSVTQYSWKQQMEIDLSQANCIAIDRDLSGKTVQVWDSDANSSCDFRGTLSS
                     VDGTGSISVTSNYVSSNALTGKLIKMTENNNCQYVKFRSY"
     gene            10981..12162
                     /locus_tag="LZI70_RS20425"
                     /old_locus_tag="LZI70_20395"
     CDS             10981..12162
                     /locus_tag="LZI70_RS20425"
                     /old_locus_tag="LZI70_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004852026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="WP_255232890.1"
                     /translation="MIIKKSMIAVSLVLSWGAIAGNIDFISYDEVALEYNKQNVTVKQ
                     DSIDELIAKAEKALDASIDPVTNKTLLPASGDKHDYFSFGPYWWPNPDTKDGLPYVRR
                     DGEYNMATKSAATDKQRFIRFTKDVTDLSLAYYFTDNQAYADKAKAQLIAWFVDPNTR
                     MNPNLNHAQAIPGIVDGRGIGIIDSRLLIPVLDSINILKQQLTPQDYQAMVDWFSDFN
                     HWLLTSNHGFEEDNWHNNHGTWYDAQVAAFAIFVGNEDIAKQRLRITQMRRIGSQFDM
                     NGRQHGELERTRPWHYSNFNLEAYNHLGHFGDKLGVDVWNYEIDKHSLAKGYQYIAKH
                     ANQPDKWQYKELKGFDGSKAFVNLLYAKKAYGDPLFTDAISELSKEKVNRKKVANLLF
                     K"
     gene            12178..12852
                     /locus_tag="LZI70_RS20430"
                     /old_locus_tag="LZI70_20400"
     CDS             12178..12852
                     /locus_tag="LZI70_RS20430"
                     /old_locus_tag="LZI70_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010438391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="WP_255232891.1"
                     /translation="MSKFYKIASITVLALVSSASVSAASLDFRQEYKHESETYASRVK
                     LGAGVGNMYFGVEAMQKGKPFSEWESKGNEFDFGYKYKIDDNWMLIPGMPITFFPGHV
                     TYKPQLRAQYSFDSGVVTKLRYRHEFRNFSDDRSAEQKSKVTANVDYKYNGFQFGLEA
                     NYEKGLDDQVFFDNGDTNWDALFKLGYKGEDWSWRPYVEFANVSVSSSSSDRQLRSRI
                     GITYSF"
     gene            12925..14496
                     /locus_tag="LZI70_RS20435"
                     /old_locus_tag="LZI70_20405"
     CDS             12925..14496
                     /locus_tag="LZI70_RS20435"
                     /old_locus_tag="LZI70_20405"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_255232892.1"
                     /translation="MTLIKRGALLLVTAMLGIALIVFVSLDSIKTSLVETKKHEVASL
                     LSFAKKQASDAILQSRSPEEAEQRVITVLSTYRLGNAYIWANDHNAIARVHVRSEKLG
                     TFQSSYARHVNLLKNNEFNYVIGEVSKPNSSEKVTKVSAITMIPELKWVMGLGIYMDD
                     VEESYQQLVMRLLIYSGGILAVILLISSLIARSIFRSVGGEPSVVVSLTKRIANGQLT
                     HSVTKSAPQGSILESVGQMQTSLKQFVDKIASVSSMVNQSTDSLDRNVVATTQSLGQI
                     RSISDDTLNSMKALELSIEEINTGIDKAQSNSLQSQSLSEEGVEAIQLMEGSVEDIRT
                     SLDSSLQSFGLLAAKYEQITGVVEVIQGIAEQTNLLALNAAIEAARAGEQGRGFAVVA
                     DEVRSLAARTSDATVEISNMSAHILSESEHVSNELRSLLEKISQSEEKSEQVSVIFSQ
                     VKQGASDVQSIVSDLKRSTKTELAISEQVYLSIEQLLKIQEANLSRIEQFSLSSSELK
                     QLSEDLQSNSHGFEK"
     gene            complement(14525..15247)
                     /locus_tag="LZI70_RS20440"
                     /old_locus_tag="LZI70_20410"
     CDS             complement(14525..15247)
                     /locus_tag="LZI70_RS20440"
                     /old_locus_tag="LZI70_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011221747.1"
                     /GO_function="GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="WP_255232893.1"
                     /translation="MLTKTLHKLFSNGDNIEDLHQSMRAKHWPSRALEHYFAHPFPNA
                     ERPVSEQQFVALDFETTGLEPDSDRILSIGTVELSYEEIELTSVSEVILRQDVTIRPE
                     SAVVHEIMPMQVQEQGVEAELALDSLIEHLAGKVIIAHSACIEYSFLSSYLKRHYQLQ
                     KLPCYMIDTLLLEKRFSYLGKNRGHASFQLDDLRQHYNLPDYQAHSAASDALGCAELF
                     LAQVKRLKLCHRPLTDVIYKPC"
     gene            complement(15249..17117)
                     /locus_tag="LZI70_RS20445"
                     /old_locus_tag="LZI70_20415"
     CDS             complement(15249..17117)
                     /locus_tag="LZI70_RS20445"
                     /old_locus_tag="LZI70_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011081826.1"
                     /GO_function="GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="WP_255232894.1"
                     /translation="MGTSASNNIFDFLIHLDPFDLLPRQVVKSLASSVTVKYLAKGEC
                     IEFHSMCQKRYLYVIRKGAIEQRTQINKLRARLGEEDQFGFTFLEPLKEAEDGYRAEA
                     IEDSLLYLIPHANIQETCELHPEFKDYFAATAKTRLNSAIRSIVNKEEKSLFFRTVGE
                     VASENITVSDYCESIMDVAKAMCGKRRSSCAVVLKGREIVGLVTDRDMTQHVVARGVD
                     TQRPIHEVMTRNPILINSEDKVIQAISLMLQYNIRCLPVIAKHEVKGLLTTTHLVHNH
                     RTQSLFLIEKIKYANSISDLANLRDEKETIFQALVESGVSAEIHGQVMSMIMDAFNRR
                     IIQLTEEVLGPPPCDYAWMVAGSHARNEVHMLSDQDNALVLAEDATEDDRFYFAYFAM
                     RVCNGLAAVGYPLCSGKYMAATAKWNQNVGRWKDYYTKWVASPEYNKLLSISVFMEIR
                     CVYGNAELVDTLQNHLHETIHANPRFIPALTRDAIETSPPLGIFNSLVLEKSGDNTKS
                     LNIKKFALNLIIDLARIYSLSAGGSLTGTEERFRYAVEQGTLSQDSCDNIIGAYRFLM
                     QVRFRHQLNAQLRGDSPDNNIRPDEFSSFERKHLKEAFKIISELQDVAKLKFMSGF"
     gene            complement(17203..17985)
                     /gene="kdgR"
                     /locus_tag="LZI70_RS20450"
                     /old_locus_tag="LZI70_20420"
     CDS             complement(17203..17985)
                     /gene="kdgR"
                     /locus_tag="LZI70_RS20450"
                     /old_locus_tag="LZI70_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006069853.1"
                     /GO_function="GO:0003700 - DNA-binding transcription
                     factor activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="WP_255232895.1"
                     /translation="MEKSTQPEAVSSVLKVFNILSALADQKEIGVSELSQRLMMSKAT
                     TYRFLQTMKTLGFVEQEGEADKYSLTLKLFELGSKALEHVDLVDLANAEMAKISKIIN
                     ETVHLGSIDEDSIIYLHKIDSSYHLRMHSRIGRRNPLYSTAIGKVLMSHMEDQDVRDL
                     LANVEFKKHTQRTHENVDQLLEELDLVREQHFGEDNEEQEPGLRCIAVPIYDRFGDPI
                     AAISVSFPTIRFEESRKREYIALLHTAGRNVSEHLGYHNYPV"
     gene            18245..19006
                     /gene="kduD"
                     /locus_tag="LZI70_RS20455"
                     /old_locus_tag="LZI70_20425"
     CDS             18245..19006
                     /gene="kduD"
                     /locus_tag="LZI70_RS20455"
                     /old_locus_tag="LZI70_20425"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431375.1"
                     /GO_function="GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA]; GO:0051287 - NAD
                     binding [Evidence IEA]; GO:0016491 - oxidoreductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="WP_255232896.1"
                     /translation="MILDSFSLEGKVAVVTGCDTGLGQGMALGLAAAGCKVVGVNYVE
                     PTETIEKMNAAGYTFLDVRANLLKQDDIPHIIDSTVTEFGKVDILVNNAGIIRREDAI
                     DFSEQNWDDVMNINSKTVYFMSQAVARQFIAQGTGGKIINIASMLSFQGGIRVPSYTA
                     SKSAVMGITRAMANEWARHNINVNAIAPGYMATNNTAALRADEDRNAAILERIPADRW
                     GTPEDVAGPCVFLASDAASYINGYTVAVDGGWLAR"
     gene            19059..19619
                     /locus_tag="LZI70_RS20460"
                     /old_locus_tag="LZI70_20430"
     CDS             19059..19619
                     /locus_tag="LZI70_RS20460"
                     /old_locus_tag="LZI70_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014233475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="WP_255232897.1"
                     /translation="MEKNTIAQILGFLSFFLGVCTFYQKDDKKLKVLMLVFHLNHLLH
                     FFLLGSLVSVVSSGLSALRTATAIYISSKRVAAIFIVISIASGFYIMESPQDIWPILG
                     TVIGTFSVFVLKGVAMRIGFLVGALCWLANNFIIGSIGGTLLEMTLISVNLMTTAKLL
                     REQKKVKLVDNEEKEDPCLYTTKTSN"
     gene            19589..19909
                     /locus_tag="LZI70_RS20465"
                     /old_locus_tag="LZI70_20435"
     CDS             19589..19909
                     /locus_tag="LZI70_RS20465"
                     /old_locus_tag="LZI70_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_255232898.1"
                     /translation="MFVYNKDIKLEDLGDGISRKVLAHSDNMMAVEVHFEKGAIGSMH
                     NHPHEQLTYVLSGKFEFTIGDEKKIVEAGDTMYKEPNIEHGCVCLEAGVLIDNFTPMR
                     KDFV"
     gene            19972..20607
                     /locus_tag="LZI70_RS20470"
                     /old_locus_tag="LZI70_20440"
     CDS             19972..20607
                     /locus_tag="LZI70_RS20470"
                     /old_locus_tag="LZI70_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010431365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="WP_255232899.1"
                     /translation="MKIALTMENSQAPKNAMVAAELNTVAGGLGHEVFNVGMTDENDH
                     HLTYIHLGIQASILLNSKAVDFIVTGCGTGQGALMASNLHPGVVCGYCLEPSDAFLFN
                     QINNGNAIALAFAKGFGWAGELNVRYIFEKAFTGARGEGYPIERAAPQQANAAILNNV
                     KAAVCKDVVDSLKAIDQDLVKQAVGSAQFQECFFANCQVTEIAEYVRSLID"
     gene            complement(20918..21541)
                     /locus_tag="LZI70_RS20475"
                     /old_locus_tag="LZI70_20445"
     CDS             complement(20918..21541)
                     /locus_tag="LZI70_RS20475"
                     /old_locus_tag="LZI70_20445"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017033514.1"
                     /GO_function="GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]; GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA]; GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="WP_255232900.1"
                     /translation="MTTLNEQLASLKVIPVIAINKVEDAIPLGKALVDNGMPCAEITF
                     RTECAADAIAAMRQAYPEMLIGAGTVLTNDQVDQAIEAGVDFIVSPGFNPRTVQYCID
                     KNVPIVPGVNNPSLVEQAMEMGLRTLKFFPAEPSGGVGMLKALTAVYPVKFMPTGGVS
                     LKNVDDYLSIKSVLACGGTWMVPTNLIDEGRWDELGQLVKDAVAHVN"
ORIGIN      
        1 caatatgatc acattacgtc atgcaaacga tcgaggaaca gcaaacttcg gttggttaga
       61 tagcaaacat acattctcat ttggtcacta ctacgatcca aaacacatgg ggttttcggc
      121 tttgcgtgtg atcaatgatg acctagtcaa gccgggcgcg ggcttcgata cccatggaca
      181 ccgtgacatg gagatcatta gttacgtcct agagggtgtg attgagcaca aagatagcga
      241 aggtaatgtt caaacgctac ctgctggaga gttccaactt atgtctgctg ggagtggtat
      301 ttatcacagt gagtacaacg gttcacagag tgaggactta aaattcctac aaatttggat
      361 tcaaccaaat acctctggta acaagccggg ctaccaacag aaagattttg gtaagaacac
      421 tggccttacg gcgatagcga cccccgatgg tcgcgatggt accttacata taaaacaaga
      481 tgcttcactg ttccaactta tccttgaacc gggacagagt attgaactgc cgattaccaa
      541 tggccggaag gtatatgttc atcaggtcac tgggcagcta tctgttgatg aggtgcttct
      601 cgatgcagga gatggcgcta aagtggaagc tcagaatcag gtgaattttg ctaaccaatc
      661 aagtgagtca gtaacggcac tggtgtttga tctgccgtag tgaagctcta accctcctgc
      721 ctaattgaca aaagtagccc cagaactcaa cagtttgggg ctgttttcgt tttaaaaggt
      781 tggaggatta ccattcaacc tctgaaccat cgaatacttt gaaactacca gactcttcga
      841 acggcaggga ttcctttcct attgctgatg atatgaggcc ctctcgttta aactgaggtc
      901 gcagtaatct gatatgttct agattatctg aatagactca aactctcgat tggagttcca
      961 agttacataa ggttttcata gaactgaagc cagctcttgg ttgaaagagc tggctttatc
     1021 aattaactga atgtcagttt ctcgtgttca gggtgccaac tgcgagttcg caatgaatgc
     1081 gaatgcaagt tataggtgat ggtggttttg ttgccatctt gattaatact gaatgtgatt
     1141 tcagtgtcgc tccaagagac gtcttcaagt agaagctctg cgctttccga taggtcgtgc
     1201 agcaccaagg tgtttaacgc ggtcgcatta gagcgaatca aggtgtatgc aaggtttgaa
     1261 gtcgcagggt tatctggcgc ttcaccaatc aagacatcac atggcgaact gaataccatt
     1321 ctctggctaa atggtgtttg tccttcaatg gtatagcgat gggagtgtgc tgaaaccgag
     1381 cttaactgac gtgtatttgt catccggttg agtgcgcccg gcaacgggtt agagcagttc
     1441 tcaaagttat ccgtgtcggt atgctcaccg cgaacgtggt aggtcaaatc caactgttgt
     1501 ttatgtggat tggcgatcag gttggtttca acataggctt cgccaacaca gaataagata
     1561 cgtctgaaca taacgttctg atatgcctga ctatcccatt gctcgatggt gtgactatcc
     1621 acttctgctg gtggttggtt ccaatctgca agcgtatcaa tgactttgca aatcggtgag
     1681 tcagagaagt gaagcagtat agggtcgatt ggcgattggt tctgctgatt cacaaccaga
     1741 gtgttatgcg tagcggtatt cttgtaatag ccgtagtgta gctctgcgcc atatccggtg
     1801 gtgccgagat cgggcaggat ctccttgcca ttgcgaacaa tgatcaagcc caatcgatcg
     1861 tagtgatcgt gttcaccgcc atacggactg tgtttgagaa ggagcgaatg atcattgctc
     1921 tggtcgtaat gaatcgtcac gccagcttga gtggtatgga tagattgaca ttgcagtggc
     1981 gccgcctttg gcagttcatc gacaccgtaa agtaatgcgt cgatgttatc tctagagata
     2041 ttttgataga tgctgcttag aacacttgca aattcgccac acgggtactg cgcgtaggca
     2101 aactcaaaca agtgtgaatg agtaatgcgt tcttgcccgg caatgcagtc attgagacgc
     2161 gggaagtcac ccgtattcag taccaagtta agtgggaact tgagcatctt aacaaaatta
     2221 gggttagagc ttactgaatg aggcgtagag taggcgatct tttcaaagtt caacagcgct
     2281 tgtaacgcgt aatagtgata gtggattgag ccttcaaacc acatcccctc ttctgagcag
     2341 ccgtggttga gttgatagtg gataccgtag tcagtattca gagcaaaatc gatgtagcgg
     2401 ctttcttcta agataagccc gattacacca atcgtggcgt tgattttcat ctcatgattg
     2461 tggagttggt cagttcggta ctccattaag aactcagccc cttcacgcaa taggcgctgt
     2521 tcgatgtacg actgctcgtc cgaggtcagt tgctcacgaa tgaagtcata accgcgagca
     2581 agatctaggt gacagtttgc ctcgcatagg gtttgcgcat tggctttacc ggggccgttg
     2641 tatgggatac cgccatgaac ctggtaatcc ggatagtatt tagcggattg gagtaggatg
     2701 tcttttacct tgtcaaagaa acgttgctca ccggttagat tccacagtaa gccaaggtcg
     2761 tagcacgctt ttgcattcag gccatttagc catcgccacc aagcgccgtc ataaggttca
     2821 cctgtaaact ctttgccgtc aacagggcag cagtgtgaga ggggtttttc acgatcccac
     2881 gttaggcgga cgccatgttc agggcaaaag taatagaggt tccaagtcgc acgaccatca
     2941 gggggaacca aggtgtcgct attaagcaca acacgattgt cttctattag ttgggcgatg
     3001 gtttgctcag aagcgcgctg tttgatggat tggatttgtt tttcgctaaa ctggatcatt
     3061 gagcatcccc tgtgagttcg gattgaggaa aattaaagta gcgctctgcg ttgtaataac
     3121 agatgttttc gatggtttct ttgagcagag cgtcattatt tggaagctcg ccattctcaa
     3181 cccatgaggc gagttggttg cagagtacac ggcggaaata ttcgtgacga ctcatggaaa
     3241 atacattgcg agaatcggtc aacataccga tgaatcgccc tagagctcct aagttcttta
     3301 atgtggtgag ctgcgcttcc atgccgtctt tgtggtcgtt aaaccaccat gcagcgccaa
     3361 attggatttt tccagcggcg gagtcactgt cttgaaaagc tccggcaata caactgagta
     3421 acgcattgtg attcggattg atgctgtaca ataccgtctt cggtagctgg ttggttaaat
     3481 ctaattgact gagaagttgg gttaggtttt cggcaatgag gcgatcattc ataacgctaa
     3541 atccggtgcc accgccaagc tcctgttttc tgcgttcgtt gacattaacc agaacgccta
     3601 tatggagctg cattgcccag ccacggctgt gatagtgttg acctaaatgg ttgaatagat
     3661 aagagctaag ctgtgtgctt tcggtatctt tgaggacttg cccggatagc agcttctcaa
     3721 acagttgttg agccgtattg tctgagcaag gagcaaaatt cacctgagtg agcccaagat
     3781 cggacaagcg gcagccagcg tcatgaaatg cttcgactcg ctttgaaatt gcgttgagga
     3841 agtcattgat ggaggcaata tgaagagcag tgattgtgct caatgtatca atcatattgc
     3901 gaaatgcagt tggcgagtca aatgcaaaga gatcgtctgc tctaaatgta ggaagcacct
     3961 caacgtcaaa gtcggactcg gcaatcagtt ggtgatagcg aagatcggag agcggtgagt
     4021 ctgtcgtgca aagggcggtg acgttgcact gctttaatac attctgagtt gtgagtctgg
     4081 atgtagcaag ctcggcttca cagtgtgacc aaatagcatc gcaattgtct gggttcaata
     4141 tcagtgaaca attgaagaat cgctctagct ctaggtgaga ccactggtaa agcgggttgc
     4201 cgagtaggta aggggccgtt tgacaccact ttgcgaactt ttccatatcg gaagcgtcgc
     4261 cagttatata gtgttcttca attccattgc tgcgcatcgc tcgccataaa taatgatcac
     4321 tatgcagcca gcattcagcg atgttactcg cacggtggtt ttcccagatc tgctttgcat
     4381 ctaggtgatt gtgataatca ataatcggca tggctttggc atagtcatga aacaagtggc
     4441 gagccaaatc tgaggttaga agataatcgt catggatgta atggttcatg ggtttcatct
     4501 cgaaatattt tttgaaaact caacagtgac attggttaca atgatgaaat aggtcactgg
     4561 ttgctaacgg tttgctaaac gcaaaatagg ggctggtggc cagccccttg tcgctacgtt
     4621 aactttgagt ttgcaccttg agtactttgg tctctttggc gctttttaac agtaaagcgc
     4681 ctacggttag taccacgccg ccacagaaca agaatgccat gcgaccccag aatggatttg
     4741 gtatcagtac catagcgaca accaaaacac cgaagattaa gatgagctta ccaagcatgc
     4801 tgcgctgctg gcggtcgacc gcatcttgtc cctcttcctc gataacaggg gttgccacat
     4861 ccgtccaaaa ctcgttaagt tctgttttac gttcgccttg tggctcttta tagaacttag
     4921 tggttaggca gaagaaaccg ccagtgaaca caaggtgacc aatgatgcca ttcattaccg
     4981 ttaggtctga tgcctcgcga ctggtgaagc cgccctcgat accgagtagg tttgcgatga
     5041 catctggtgt gataacaaag ctcaccatgt aagagaccac cataccgaca gttagcgttg
     5101 cccaagctgc ccaatctggt gtcttcttaa tgaacatgcc gaagaacaga ggaactagga
     5161 taggcatttg tagcaatgta cttacgtaca tcatggcatc aaataggctc aatccacgaa
     5221 gcgagttgat gaatagagct accgcgataa tcagagcgcc aaacaccatg gtgacgccct
     5281 ggctgacacg catcagtttt ttctcagatg ctttcttgtt gatgattgga ctgtagaagt
     5341 tacgaacaaa aacacccgcg ttacgattga ggcctgagtc cattgacgac atggttgccg
     5401 caaataccgc tgacataagc agaccgacca taccgacagg catcacacgt tcaacgaata
     5461 ccagataaac cgcatccgct gctttgccgc caagcgcgtc tgcgtgggct agtgcagcat
     5521 cagggtaaag aaccgcagtc acccatggcg gcatgaacca aatcgcagga cctattagca
     5581 taagaacaaa ggctagcagt gccgctttac gcgcattggt cgagtctttg gccgtcaaga
     5641 atctgtatga gtcttgcatg ttgttgatgt tttgaagctg tttaacaaac atgaaaatga
     5701 accatgccac gaacagtaac gggtagttca tgttcgcacc cataattgag tctgctggga
     5761 actgttcaat tagattactc ggacctccaa tttttataag ggctgcaacg gcacacacga
     5821 cggtgaccgc cataatgacg accatttgca caacgtcgga cgcaaccaca ccccaagagc
     5881 cgcctataac ggaaataaac agaacaatca gaccggtcac aacaatggtt gtctcaatat
     5941 cgattttgaa tactgcacta acgaatagcg ctagaccgtt gagccaaata cccgtgtaaa
     6001 cgatgctggt tggcatggtt gcccaagtaa agacttgctc gtttgtcgtg ccaaatcgac
     6061 gtcgtacacc ttctactggg gttacaacgc gcatttgacg gaaacggtag gagaaaaaca
     6121 accaagaaag taggaagccg aatgcgttgg ccatgaaaat gaccatgata gggaaaccat
     6181 cggtaaaggc tttgcctgct gctccagtga acgtccatgc actgaattga gtcataaagg
     6241 ctgtggagcc caccatccac cacagcattt tgcctccccc acgaaagtaa tcgctggttg
     6301 agttgcctgc tagacgcttg aatatgacac ctacagcgat cataaagacg aagtagacac
     6361 cgacaacaac aatgtcaata ttcataacac ttacctctaa agttaaattg attaatttga
     6421 aaaaataaaa cgatggttca atattgcttt aggtaggtgt taattaccaa aaaaactgtg
     6481 cgcttttgtg atgaatgaaa cggtttttca tttttttatt cggattgatg ctttttcttt
     6541 catggtttat atctaactta aagagctccc cccaattttg ccttcaatcc ttcaatgagt
     6601 cttggctgtg gttattacac aggcagtaat agactcgtac ttttctttta tgttgctcgt
     6661 taactacaca gtacggactc atttgtggac gcctatttga cctttttaca actgtatcag
     6721 taacgcctgg ttgtgcatgt tgcgcattct ttgaccgcgt taacataaaa caacacgccc
     6781 agaaataatt agttacataa gtcacataat gacctaaacg agctttataa aaacagcatt
     6841 tactaaataa tgaaacggag ttttgaaaat taatcttaaa aagtaaagaa atgtaaaagg
     6901 agttcagaat gaatgtcgat atgctcgtcg tgggcgttta ctttatcttc atgatagcga
     6961 taggcgtcat ctttaagcgc tttgcgggta gttccactag cgactatttc cgaggcggcg
     7021 gtaaaatgct gtggtggatg gtgggctcca cagcctttat gacccaattc agtgcatgga
     7081 cgttcactgg agcagcaggc aaagccttta ccgatggttt ccctatcatg gttgttttta
     7141 tggccaacgc atttggcttc ctactttctt ggttgttttt ctcctaccgt ttccgtcaaa
     7201 tgcgcgttgt aacccctata gagggggtcc gtcgtcgctt cggtggcacc aatgaacagg
     7261 tatttacttg ggcaaccatg ccaaccagca tcgtttacac gggtatttgg ctcaacggtc
     7321 tagcactttt cgtaagcgcg gtatttaaag tggacatcga gacaaccatt gttgtgaccg
     7381 gccttatcgt tctattcatt tctgtaattg gcggtgcttg gggtgtggtt gcgtccgact
     7441 ttgtgcaaat ggtcgtcatt atggcggtca ccgtcgtttg tgccattgct gctttagtta
     7501 agattggcgg tccaacaaac cttattgagc agtttcccgc tgattcaatt atgggttcag
     7561 atatgaatta tccattactg ttcgtggcat ggttcatctt catgtttgtt aaacagcttc
     7621 aaaacatcaa caacatgcaa gactcatatc gtttcctaac tgcgaaagac tcgaccaatg
     7681 cgcgtaaagc cgcactactg gcgtttgttc ttatgttaat cggtcctgca atttggttcc
     7741 taccaccttg ggtaacggca gtcatgtacc cagatgcagc tactgttcat gctgcagaac
     7801 tgggtaaaaa agctgcggat gcggtatatc tagtgttcgt agagaacgca atgcctgttg
     7861 gtatggttgg cttgcttatg tctgcggtat ttgcagcgac catgtcgtca atggattcag
     7921 gtcttaaccg taactctggc atctttgttc gcaacttcta tgcgccaatc cttgataaaa
     7981 aggctgacga caaaaaacta atgcgcgtaa gccaaattgt cactatgtgt tttggtggtc
     8041 tcattattct cgtcgcactg tttatcaact cgcttcgtgg attgagtcta tttgatgcca
     8101 tgatgtacgt gagtacattg ctgcagatgc caattctcgt accactattc tttggtatct
     8161 tcattaagaa gacaccagat tgggctgcat gggcgacact ggccgtcgga atcgtggtct
     8221 cttacatggt gagctttgtg attacggctg atgtggtggc aaactggctg aacctagata
     8281 caccatttac cggtcgtgaa gcgaaagatc ttaaggtgct actaggcatt atgggtcacc
     8341 tattcatcac tggtggtttc ttctgcctca caactcgatt ctacaaagag ccagtgggtg
     8401 agcgtaagtc tgaactgacg gagttctgga atgatgttgc gactccagtt gtagaagaag
     8461 ctggtcaaga cgagctagat cgtcaacaac gcaatatgct aggtaaattg attcttgtat
     8521 ttggccttct agtgacacta atggtactta tccctaatcc attctgggga cgaatggcct
     8581 ttgtcttctg tggtgcggtc attgtgaccg taggtagttt gcttcttaag agtgccaaac
     8641 aggcggataa gctaacagca agtagtgcta catagcctga ttaaccccga ttgaggagct
     8701 acgcagttca cagtagctcc tttttttaac cagtaatgaa acgaagtaca ccttaaacac
     8761 ggaagggaga tccaaacgtg aaaatgaacg tgaacacact cgccatactt atacctctta
     8821 ctctatatag tgcccctggt ctttctgctc caggttataa acaaggtgca acgttaccta
     8881 cgacatttcc aaacactatt tatgctgttg atcatggcat tatcgcagat gacgggatcg
     8941 atgattctgc agcactgcaa gccatcattg ataatgaggt tactgcctcg acgagtaaca
     9001 atactttgat tgtactgcca aagggcacta tcaacctgag cgatgaaatc catatcgaca
     9061 agtcaggggt ggtaatcaaa ggggcgggca gcgatcccga tacgggtacg caaattgtcg
     9121 tcaaatcttg gtccccttac ggagtggatg ccagcaatgc gcctgatttt gataagaaat
     9181 attggcctgg ttttggcgta ttccgtgtcg agactcgaga aaaacatgcc aaagagcctg
     9241 cttacgaggg gagtgttaac tttcattgga aacatagcat tgagtttgca cagccggcga
     9301 aaatgggtga taccaaaatt gtcttagaat caggaaaagc tggcgaattt agtcagggtg
     9361 atcttatata tgttggcgcc gctactgacc gagcgttttt ggatgaagga caagtgccgg
     9421 aatcgagaag aagtgctagt cacattaaaa ctggccacat gcgcacacag atttttaaag
     9481 taacggaggt aaataccagc agtaatacag tcacccttga tggcgcgctt gaatttgata
     9541 ttccgctaaa caatgagggt ggttataaca gccgtgttat gccagtgcgt gcaatagaaa
     9601 attttggttt acaagatttc tttctaacta tggacataga aggtagtcac tgtgaagggt
     9661 acaaccgcaa ccaattctcg gagagtaacc ctaatggtgt cttgtatcgt tatgagaacg
     9721 tatgtgcaca agatgcgatt cacggcatca tactcaagtg ggcgtataac ggctgggtcg
     9781 ataatgtccg tatcgacatg attggctcgc accctattgt tagtgagttt gctaaagcag
     9841 tcaccatttc caacaatact atcgacggtt catggaacaa gggtgctggt ggtaatggtt
     9901 atgtacgtgg ttcaaagtta tacaatagct ggatccacaa taacgatatt cgaaacattc
     9961 gacaccttgc tctacagtgg tcagcaacag gaaatatcgt tgagaacaat acactgaatg
    10021 ttgatatgaa cttccacggt ggctgggaac gtaacaattt ggttcgttct aatcatattg
    10081 cagtgccgtt tgagcatcga agttggtcaa atggagcgcc agaatctgga gcaacgtggc
    10141 aacctatttg ggtcggttct ggcgatcatg caagcaagtg gtcaggacca actggaccaa
    10201 ataacgtcct agttaataac acgctgaaga aagcaaagaa ctcgggtgac agcattactc
    10261 gatgggggct gtttgatgaa ccggatgtcg agtacgcgct caactgggat ggtagccaat
    10321 ataaacacct gaatatcaac ggggagccaa tagcgacttg gagtcaaaca ttggcagagg
    10381 gcgttcatca gcagatcccg cagtctggtg ttactgttgc aggcggatca tggccaccag
    10441 aaggtgtgga tccggaagag cctgttgatc cggtcgatcc gccggtttct gaggggtgtg
    10501 attcggtgac ccaatatagt tggaagcagc aaatggaaat agacctttct caagccaatt
    10561 gtatagcgat tgatagagat ctctctggga aaacggtaca agtttgggat agtgatgcaa
    10621 acagcagttg tgacttcaga ggtacgttat ccagtgtgga tggtactggc tcaattagcg
    10681 tgacgtcaaa ctatgtgagc agcaatgctc ttacaggtaa gctgattaaa atgacagaaa
    10741 ataataattg tcagtatgtt aagtttaggt cctattgatt tcaaatgggt attaaataaa
    10801 aaaggagctc atcgagagct ccttttttat ttaataaccc tcactcataa gatttaaaaa
    10861 gtttgataaa aatcacaatt actagagtgc atataacatc tatgtaatta aatgatcttg
    10921 atcttgtttt tgaaccgttg tttcaattaa tattgaattt aatttcatta aggggttttt
    10981 atgattatca agaaaagtat gattgctgtt tctcttgttc tttcttgggg ggcaatagct
    11041 gggaatatag atttcatctc atacgatgaa gtcgcacttg aatataacaa gcaaaatgta
    11101 acggtgaagc aagattcaat tgacgagtta atcgcaaagg ctgaaaaagc attggatgca
    11161 tcaatagatc ctgtgactaa caaaacgtta ttaccggcga gtggcgacaa gcatgactat
    11221 ttcagcttcg gaccttattg gtggccgaac cctgatacca aagatggtct accttatgtc
    11281 cgccgcgacg gtgaatataa tatggcgacc aaaagtgcag caacggacaa acagcgcttt
    11341 atccgtttta ccaaagacgt gacagacttg agccttgctt attacttcac tgataatcaa
    11401 gcttacgctg acaaagcgaa agctcagctt atcgcctggt ttgtcgaccc gaatacacga
    11461 atgaatccaa acttaaacca tgcacaagcg atacctggaa ttgttgatgg gcgtggtatc
    11521 ggcattattg atagccgctt gctgatacca gttttagata gtatcaatat tttaaaacaa
    11581 cagcttaccc cacaagatta tcaggctatg gttgattggt ttagtgattt taatcactgg
    11641 ttgctaacca gtaatcatgg ttttgaagag gacaattggc ataacaatca tggcacatgg
    11701 tatgacgcac aagtcgctgc ctttgccatc tttgtgggta acgaagatat tgctaagcag
    11761 cgtctgagaa tcacacaaat gcgccgaatt ggtagtcagt ttgatatgaa tggtcgtcag
    11821 catggagagc tagaacgcac gcgtccttgg cattattcaa acttcaatct tgaagcctac
    11881 aatcacctag ggcacttcgg agataagctc ggtgttgatg tttggaatta tgaaattgat
    11941 aaacattctc tagcgaaagg ttatcaatat attgcaaagc atgccaatca acctgataaa
    12001 tggcaataca aagaactaaa aggatttgat ggttctaaag ctttcgtaaa tttactttat
    12061 gcgaaaaaag catacggtga tccgttattt accgatgcga tctctgaatt aagtaaagag
    12121 aaggtaaata gaaaaaaagt agctaatcta ttgtttaaat aaaaaaggaa ttatactgtg
    12181 tctaagtttt ataaaatagc ttcaattacg gttttggcat tagtttcgag tgcctctgtt
    12241 tctgctgcat ctcttgattt ccgtcaagaa tacaaacacg aaagtgaaac ttatgcgagc
    12301 cgcgttaaac ttggtgcggg cgtaggaaat atgtactttg gtgttgaagc aatgcagaaa
    12361 ggtaagccgt tttcagaatg ggaatcgaaa ggcaatgaat ttgacttcgg ctataaatat
    12421 aaaattgatg ataattggat gctgattcca ggtatgccaa ttaccttctt ccctggtcac
    12481 gtgacctata aacctcagct tcgcgctcag tactcttttg attcaggggt tgttactaag
    12541 cttcgctacc gccatgagtt ccgtaacttc tctgatgatc gctctgccga gcaaaagagc
    12601 aaagtcacgg ccaacgtcga ttacaaatac aacggttttc agtttggtct agaggcgaac
    12661 tacgagaaag gtttggatga ccaagtattc tttgataacg gcgacaccaa ttgggatgca
    12721 cttttcaaac ttggctacaa aggcgaagac tggagctggc gtccatatgt ggagttcgcc
    12781 aatgtttcag tttcgagctc ttcaagcgat cgtcaacttc gaagccgtat cggcattact
    12841 tatagcttct aggcgtttac tgtaattgac tgtccgtttg gccacactag gttgttagtg
    12901 tggccatttt tatagtgagg gattatgaca ctaataaaaa gaggtgcact gctgttagta
    12961 acagccatgt tgggtatcgc gctgattgtt tttgtaagct tggactcaat caagacgtca
    13021 ttggttgaga ctaaaaagca tgaagttgct tcgttacttt catttgcaaa gaaacaagca
    13081 tcagatgcta ttttacaaag tcgttctccg gaagaagctg agcagcgtgt aattactgtg
    13141 ttgtctacct atcgtctggg aaatgcttac atttgggcga atgatcacaa tgccattgct
    13201 cgcgtgcatg tacgtagcga aaaactggga acgtttcaaa gctcctacgc tcgccatgta
    13261 aatctgctta aaaataatga gttcaattac gtaatcggtg aagtgagtaa gcctaactcg
    13321 tcggaaaagg tgaccaaggt gagtgcgatc accatgattc ccgagcttaa atgggttatg
    13381 gggcttggga tctacatgga cgatgttgaa gagagttatc aacaactcgt gatgcgcctt
    13441 ctaatctaca gtggtgggat tctagcggta atattgctga ttagcagtct tattgcgcgc
    13501 agtatttttc gctcggtggg tggagagcca agcgttgttg tttctttgac gaagcgtatt
    13561 gcaaatgggc agttaactca ctctgtgacg aaatccgctc cgcagggaag tattttagaa
    13621 tccgtaggcc agatgcaaac gtccctcaaa cagtttgttg ataaaatcgc ctcggtttca
    13681 tcaatggtta atcagtctac agacagtcta gatcgaaatg ttgttgcgac gacgcaaagc
    13741 ctagggcaaa tacgctcgat ttcggatgat acgctcaatt ctatgaaagc gcttgaactg
    13801 agtatagagg aaatcaatac aggcattgat aaagcacaat cgaatagttt gcagtctcaa
    13861 tcattgtctg aagaaggagt agaggctatt caactgatgg agggttctgt cgaggacatt
    13921 cgcactagcc ttgatagttc actacaaagc tttggcctgt tggcagcaaa gtatgaacag
    13981 ataaccgggg ttgttgaggt gatccaaggc atcgctgaac agacaaactt gcttgcccta
    14041 aacgctgcga tagaagccgc gagagctggc gaacaggggc gtggctttgc tgtcgttgct
    14101 gatgaagttc gaagtcttgc cgccagaacg tccgatgcaa cggtagaaat atctaatatg
    14161 agcgcgcata tcttgtcgga gtctgaacat gtttccaatg aattgagatc gcttctcgaa
    14221 aaaatctctc aaagcgaaga gaaatcggaa caagtaagtg tgatttttag tcaagttaag
    14281 caaggtgcga gtgacgttca gagcattgtt tctgacttaa aacggtcaac aaagactgaa
    14341 ctggctattt ctgagcaggt ttatctttct attgagcaac ttctcaagat tcaagaggca
    14401 aacttgagtc gaatagaaca gttttctttg agctcaagtg agcttaaaca gctttcggaa
    14461 gatctacaat ccaatagcca tggctttgaa aaataacagc atgtggcagt tcaagctgcc
    14521 acacttaaca aggcttatat atcacgtcgg tgagtgggcg atggcagagt ttaagtcttt
    14581 ttacttgtgc caaaaagagc tcagcgcaac cgagtgcatc actggctgct gaatgagctt
    14641 gataatcggg aaggttgtag tgttggcgca gatcatcaag ttggaagctg gcatggccgc
    14701 gattcttacc caagtagcta aatcgcttct caagcaatag agtgtctatc atatagcacg
    14761 gcagtttttg cagttggtag tgccttttta gatacgacga taaaaagcta tattcaatac
    14821 atgcactatg agcgatgata actttacctg caagatgttc gatcaagcta tcgagcgcaa
    14881 gctcagcctc aactccttgt tcttgaactt gcattggcat aatttcatga acgaccgcgc
    14941 tttccggtcg gatagtgaca tcttggcgca aaatcacttc gctgacactg gttagttcta
    15001 tctcttcgta gctcaactcc accgttccaa tagacaggat tctatctgag tctggttcca
    15061 gccccgttgt ttcaaaatcg agggcgacga actgttgctc ggaaactggg cgctctgcat
    15121 tgggaaacgg gtgggcaaag tagtgctcta gtgcacgaga tggccaatgc ttggccctca
    15181 tggactggtg caaatcttcg atgttgtcgc cattagaaaa tagcttatgt aaggttttgg
    15241 tcagcatgtt agaacccact catgaacttg agtttagcga cgtcttgcag ttcgctaatg
    15301 atcttgaagg cttctttaag gtgtttgcgt tcaaaactgc tgaactcgtc cggacgtata
    15361 ttgttgtcag gagaatcacc acgaagctgc gcgttgagtt ggtgtctaaa tcgcacctgc
    15421 ataagaaaac ggtaagcgcc aataatgtta tcgcagctgt cttgagataa ggtaccttgc
    15481 tcgacggcat atcgaaaacg ctcttccgtg cctgttaaag agcctccagc tgataagctg
    15541 tatatgcgag cgaggtcgat aattaggttc aaagcaaact ttttgatatt gagtgatttg
    15601 gtattgtctc cgcttttctc aagaaccaga ctgttgaaaa tgccaagtgg gggggacgtt
    15661 tcgatggcat cgcgtgtcag tgctgggata aagcgaggat ttgcatgaat ggtttcgtgc
    15721 aggtggtttt gaagcgtatc gacaagctcg gcgtttccgt agacgcagcg tatctccata
    15781 aagacactga tactaagcag tttgttgtac tcaggactcg ccacccattt cgtgtagtag
    15841 tccttccagc gaccgacatt ctgattccat ttggctgtcg cggccatgta tttaccgctg
    15901 cataaagggt agcccacggc cgctaatcca ttacaaacgc gcatcgcaaa gtaggcgaag
    15961 tagaatcggt catcctcagt ggcatcttcc gcgagaacca gagcattgtc ttgatccgac
    16021 agcatgtgca cttcgttccg cgcatgtgaa ccagctacca tccatgcata gtcacaaggc
    16081 ggcggaccga gcacctcttc ggttagttga attattcgtc tattgaacgc atccatgatc
    16141 atcgacatca cttggccatg aatctcggca ctcacgccac tctcgactaa ggcctggaaa
    16201 atggtttcct tttcgtctcg aagattcgcg agatcactta tcgagttcgc atacttgatc
    16261 ttttcaatta aaaataacga ctgcgttcga tgattgtgaa ctaggtgagt ggtggtcaat
    16321 aagcctttta cttcgtgctt ggcaatgact ggtaggcagc gaatgttgta ttgcagcatc
    16381 agtgaaatcg cttgaattac cttatcttcg ctgttaatca agataggatt gcgcgtcatg
    16441 acttcatgaa tagggcgctg ggtgtctacc cctcgagcga cgacgtgttg tgtcatatct
    16501 cgatcggtaa ccaagccgac gatttctcgc ccttttagta cgacggcaca cgaactgcgc
    16561 cgtttgccac acatggcttt agcgacgtcc atgatggatt cgcaatagtc agaaacagtg
    16621 atgttttcgc tggcaacttc gccaaccgtt cgaaagaaca ggcttttctc ttctttattg
    16681 acgatcgaac gtattgcaga gttcaatctc gtttttgctg tagcggcaaa gtaatctttg
    16741 aactctgggt gcagctcgca cgtttcttga atgtttgcgt gaggtattaa gtaaagtagt
    16801 gagtcttcaa tagcttctgc tcgatagcca tcttcggctt ctttgagtgg ttccagaaaa
    16861 gtaaacccaa attgatcctc ttctccaaga cgagccctta gtttattaat ttgggttcgc
    16921 tgttcgattg caccttttcg gattacatat agataccttt tttggcacat cgagtgaaac
    16981 tcaatacatt ctcccttagc gagatatttg accgttacgg agcttgccag tgatttgact
    17041 acctgtcttg gaagaaggtc gaagggatca aggtgaatca aaaagtcgaa aatattattt
    17101 gaagctgaag tgcccatata aatccttggt tgaaacggtg tttcattaaa ttattgggtg
    17161 gtaaaaaaag cagcacgaat gctgcttttt tctttattac ttttagacag ggtaattgtg
    17221 gtagcccagg tgctccgata cattgcgacc agctgtgtgg agaagagcga tgtactcgcg
    17281 cttgcggctc tcttcaaatc gaatagtagg aaacgaaacg gagatggctg caataggatc
    17341 accaaaacga tcgtaaattg gtacggcgat acaacgcaaa cctggttctt gctcttcatt
    17401 gtcttcacca aagtgctgct cacgaacaag gtctaactct tcaagtagtt ggtctacatt
    17461 ctcatgcgtg cgctgtgtat gctttttaaa ttcaacatta gccaaaagat ctcgaacgtc
    17521 ttggtcttcc atatgagaca tcaatacctt accaattgct gtgctgtaga gcgggtttcg
    17581 acgcccaatg cgtgagtgca tacgtagatg gtaactggag tctatcttgt gcagatagat
    17641 gatagagtct tcgtcaatgg agcctaagtg aacagtttca ttgatgatct ttgagatttt
    17701 cgccatttca gcattggcga gatcaaccag atcgacatgc tctagagctt ttgagcccag
    17761 ctcaaatagt tttagagtta gggagtattt atcggcttcc ccttcttgct caacaaaccc
    17821 gagtgtcttc atggtttgca aaaaacgata agtcgtcgcc tttgacatca tcaagcgttg
    17881 agaaagttct gatacaccaa tctctttttg gtcagctagc gccgagagaa tgttgaacac
    17941 ttttaatact gacgatacag cttccggttg agttgatttt tccatttcac gctctgagtc
    18001 attgttgata acgccaaatt atacatgaca tacttggacg agtcagtagt ttcgaaaagc
    18061 catttcattt atttaaaacc aatatttgag acattaatca catttgatat ggtgaatcta
    18121 aaaaaataaa acgcaatttc aaaaataact ttcagtggtg tcattttttg tgtaatgttg
    18181 gttttaacag agtgagcagc cccttttatt tgttcaccac acattcacta caaaggaaca
    18241 gactatgatt cttgattcat ttagccttga aggaaaggta gcagtggtaa ctggttgcga
    18301 tactggtcta ggtcaaggta tggcattagg tcttgcagcg gcaggttgta aagttgttgg
    18361 tgtcaactac gttgaaccaa ctgaaactat cgaaaaaatg aatgctgcag gctacacgtt
    18421 cctagatgtt cgcgctaacc tactaaaaca agacgatatc cctcatatca tcgacagcac
    18481 tgtgactgaa ttcggtaaag tggatatcct agtaaacaac gcaggtatca ttcgtcgtga
    18541 agatgcgatc gatttctcag agcaaaactg ggatgacgtg atgaacatca attcgaaaac
    18601 cgtttacttc atgtctcaag cagtagcacg tcagtttatt gctcaaggca ctggcggtaa
    18661 gatcatcaac atcgcttcta tgctttcttt ccaaggcggc atccgtgtac cttcttacac
    18721 tgcatctaag agtgcggtta tgggcatcac tcgtgcaatg gcaaacgagt gggctcgtca
    18781 taacatcaat gtcaacgcaa ttgcacctgg ctacatggca accaacaaca ctgcggcact
    18841 gcgtgcagat gaagatcgta acgcagctat cctagagcgt attcctgctg accgctgggg
    18901 tactccagaa gatgttgctg gcccatgtgt attcctagcg tctgacgctg cgtcttacat
    18961 caatggttac actgttgctg tagatggtgg ctggttggcg cgctaatttg gaatctcgcg
    19021 tttaaccttg gttgggcgct gacaaaagag tagatggtgt ggaaaaaaat acaattgcac
    19081 aaatcctcgg attcttgagt ttctttttag gtgtgtgcac cttttatcaa aaagacgata
    19141 agaagctgaa ggttttgatg ttggtttttc atctaaacca tctactgcat tttttcttgc
    19201 taggttcttt agtgtcggta gtcagttcag ggctttcagc acttcgaact gctaccgcga
    19261 tttatatttc atcgaaaaga gtcgcagcaa tattcatcgt catcagtata gccagtggct
    19321 tctatattat ggaatcgcca caggacatct ggccaatatt gggcactgtc atcgggacgt
    19381 tctcggtgtt tgttctcaaa ggcgttgcga tgagaatcgg ttttttggtc ggggcgttat
    19441 gctggctggc caataatttc atcataggat cgattggtgg aacgctgctg gagatgacgc
    19501 taataagcgt taacttaatg accacagcaa aattgctcag agagcaaaag aaagtgaagc
    19561 tcgtcgataa cgaagagaaa gaagacccat gtttgtatac aacaaagaca tcaaactaga
    19621 agacttaggc gacggtatct cacgtaaagt gcttgcgcac agtgacaaca tgatggccgt
    19681 tgaagtgcac tttgaaaaag gtgccattgg ttctatgcac aaccacccac atgagcagtt
    19741 gacttacgta ctgtctggta agttcgaatt cactatcggc gatgagaaga aaatcgttga
    19801 agcgggcgac accatgtaca aagagccaaa tattgagcac ggctgtgtgt gtctagaagc
    19861 tggagtgtta atcgataact tcacgccaat gcgcaaagat tttgtataag ttttaaaaag
    19921 atttcgtata agttttaaaa ttagttacgc ggttcgccgc aggagaattc aatgaaaatt
    19981 gcactaacta tggaaaacag ccaagcacct aaaaacgcta tggttgctgc tgagctaaac
    20041 acggttgctg gtggtctagg tcacgaagta ttcaatgtag gtatgactga cgaaaacgat
    20101 catcacctaa cgtacatcca cctaggtatc caagcgagca tcttactgaa ctctaaggca
    20161 gtggatttca tcgtaactgg ttgtggtaca ggtcaaggcg cactaatggc aagcaaccta
    20221 cacccaggtg tcgtttgtgg ttactgccta gagccatctg atgcgttcct attcaaccaa
    20281 atcaacaacg gtaacgcgat tgctctagcg ttcgcaaaag gttttggttg ggctggtgaa
    20341 ctgaacgttc gttacatctt cgaaaaagcg ttcactggcg ctcgtggcga aggttaccca
    20401 atcgagcgtg ctgcacctca gcaagcaaac gctgccatcc taaacaacgt gaaagcagca
    20461 gtatgtaaag acgttgttga ttcactaaaa gcgattgatc aagacttagt taagcaagct
    20521 gttggcagcg cccagttcca agagtgtttc tttgctaact gtcaagttac tgagattgca
    20581 gagtacgttc gcagtctgat tgactaattt aatttgctgt ggcttcagaa ttcgttaaac
    20641 cctaaccttt gaggtcactg cttaattaaa cgtaacacta cttattgact gttttattct
    20701 ttattttgct atccatatcg ggattttcca gccagtgttg tcactggctt ttttttattt
    20761 ttggtcaaag gcttataccc attcttttac agtaaaaaaa tagcagcttg agcagatgtt
    20821 tctggctcct taataaccca attaatgttg atcctctaaa acgcattcag ccccacggca
    20881 aaagctgttg gggctgactt tttaatcgaa gatcgaatta gttaacgtga gcaaccgcgt
    20941 ctttaaccag ttggccaagc tcatcccagc gaccttcgtc gataaggttg gttggaacca
    21001 tccaagtacc gccacaagcc aataccgatt tgatagatag gtagtcatca acattcttaa
    21061 ggcttacacc acctgttggc atgaacttta ctgggtaaac agcggttagt gcctttagca
    21121 tgcccacacc accagatggt tctgcaggga agaacttcaa tgtacgcaga cccatttcca
    21181 tagcttgctc aacaaggctt gggttgttaa cacccggtac gattggaacg tttttgtcga
    21241 tgcagtattg aactgtgcgt gggttgaacc cagggctaac gataaaatca acaccagctt
    21301 caatcgcttg atcgacttga tcgttagtaa gaacggtacc tgcaccaatt agcatctctg
    21361 ggtaagcttg acgcattgct gcaatcgcgt cagctgcaca ctcggtacgg aacgtgattt
    21421 ctgcgcaagg catgccgttg tctacgagtg ctttacctag aggaatagca tcttcaactt
    21481 tgttaatggc aattacagga attactttaa ggcttgctag ttgttcattt agagtagtca
    21541 tttatgttgt
//
