LOCUS       NZ_GG694015            17603 bp    DNA     linear   CON 15-JAN-2025
DEFINITION  Granulicatella adiacens ATCC 49175 SCAFFOLD1, whole genome shotgun
            sequence.
ACCESSION   NZ_GG694015 REGION: 628137..645739
VERSION     NZ_GG694015.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00139322
            Assembly: GCF_000160675.1
KEYWORDS    WGS; RefSeq.
SOURCE      Granulicatella adiacens ATCC 49175
  ORGANISM  Granulicatella adiacens ATCC 49175
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Carnobacteriaceae; Granulicatella.
REFERENCE   1  (bases 1 to 17603)
  AUTHORS   Muzny,D., Qin,X., Deng,J., Jiang,H., Liu,Y., Qu,J., Song,X.-Z.,
            Zhang,L., Thornton,R., Coyle,M., Francisco,L., Jackson,L.,
            Javaid,M., Korchina,V., Kovar,C., Mata,R., Mathew,T., Ngo,R.,
            Nguyen,L., Nguyen,N., Okwuonu,G., Ongeri,F., Pham,C., Simmons,D.,
            Wilczek-Boney,K., Hale,W., Jakkamsetti,A., Pham,P., Ruth,R., San
            Lucas,F., Warren,J., Zhang,J., Zhao,Z., Zhou,C., Zhu,D., Lee,S.,
            Bess,C., Blankenburg,K., Forbes,L., Fu,Q., Gubbala,S., Hirani,K.,
            Jayaseelan,J.C., Lara,F., Munidasa,M., Palculict,T., Patil,S.,
            Pu,L.-L., Saada,N., Tang,L., Weissenberger,G., Zhu,Y., Hemphill,L.,
            Shang,Y., Youmans,B., Ayvaz,T., Ross,M., Santibanez,J., Aqrawi,P.,
            Gross,S., Joshi,V., Fowler,G., Nazareth,L., Reid,J., Worley,K.,
            Petrosino,J., Highlander,S. and Gibbs,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2009) Human Genome Sequencing Center, Baylor
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   2  (bases 1 to 17603)
  AUTHORS   Qin,X., Bachman,B., Battles,P., Bell,A., Bess,C., Bickham,C.,
            Chaboub,L., Chen,D., Coyle,M., Deiros,D.R., Dinh,H., Forbes,L.,
            Fowler,G., Francisco,L., Fu,Q., Gubbala,S., Hale,W., Han,Y.,
            Hemphill,L., Highlander,S.K., Hirani,K., Hogues,M., Jackson,L.,
            Jakkamsetti,A., Javaid,M., Jiang,H., Korchina,V., Kovar,C.,
            Lara,F., Lee,S., Mata,R., Mathew,T., Moen,C., Morales,K.,
            Munidasa,M., Nazareth,L., Ngo,R., Nguyen,L., Okwuonu,G., Ongeri,F.,
            Patil,S., Petrosino,J., Pham,C., Pham,P., Pu,L.-L., Puazo,M.,
            Raj,R., Reid,J., Rouhana,J., Saada,N., Shang,Y., Simmons,D.,
            Thornton,R., Warren,J., Weissenberger,G., Zhang,J., Zhang,L.,
            Zhou,C., Zhu,D., Muzny,D., Worley,K. and Gibbs,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2009) Human Genome Sequencing Center, Baylor
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 17603)
  AUTHORS   Qin,X., Bachman,B., Battles,P., Bell,A., Bess,C., Bickham,C.,
            Chaboub,L., Chen,D., Coyle,M., Deiros,D.R., Dinh,H., Forbes,L.,
            Fowler,G., Francisco,L., Fu,Q., Gubbala,S., Hale,W., Han,Y.,
            Hemphill,L., Highlander,S.K., Hirani,K., Hogues,M., Jackson,L.,
            Jakkamsetti,A., Javaid,M., Jiang,H., Korchina,V., Kovar,C.,
            Lara,F., Lee,S., Mata,R., Mathew,T., Moen,C., Morales,K.,
            Munidasa,M., Nazareth,L., Ngo,R., Nguyen,L., Okwuonu,G., Ongeri,F.,
            Patil,S., Petrosino,J., Pham,C., Pham,P., Pu,L.-L., Puazo,M.,
            Raj,R., Reid,J., Rouhana,J., Saada,N., Shang,Y., Simmons,D.,
            Thornton,R., Warren,J., Weissenberger,G., Zhang,J., Zhang,L.,
            Zhou,C., Zhu,D., Muzny,D., Worley,K. and Gibbs,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2009) Human Genome Sequencing Center, Baylor
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            GG694015.1.
            This sequence is generated as a part of the Human Microbiome
            Project (HMP), funded by the National Institutes of Health (NIH),
            National Human Genome Research Institute (NHGRI) Large-Scale
            Sequencing Research Network and the National Institute of Allergy
            and Infectious Diseases (NIAID) Microbial Genome Sequencing Centers
            Program.  The mission of the HMP is to generate resources to enable
            the characterization of the human microbiota, the microbial
            communities living within human bodies, and analyze the role of
            these organisms in human health and disease.  See
            http://www.hmpdacc.org/ for more information.
              The sequenced strain was obtained from ATCC.  The genomic DNA was
            prepared from a single bacterial isolate.  The sequence generated
            included at least 10 fold coverage of Roche/454 Lifesciences FLX
            fragment data and at least 8 fold clone coverage of Roche/454
            Lifesciences paired-end data.
              The Roche/454 Lifesciences sequence was assembled using the
            Roche/454 Lifescience Newbler assembler.  The contigs from the
            Newbler assembly were aligned with mapping tools such as Mosaic and
            Crossmatch and these data were used for error correction for this
            version of the draft assembly.  This draft assembly meets the HMP
            draft quality standards (more than 90% of the genome is included in
            contigs, more than 90% of a core set of bacterial genes are found
            with > 30% identity and > 30% length; more than 90% of the bases in
            the assembly have more than 5 fold sequence coverage, the contig
            N50 length is greater than 5kb, the scaffold N50 length is greater
            than 20kb, and there is less than 1 gap per 5kb).
              This is a reference genome for the Human Microbiome Project.
            This project is co-owned with the Human Microbiome Project DACC.
            Sequencing Technology: 454; ABI.
            This sequence is generated as a part of the Human Microbiome
            Project (HMP), funded by the National Institutes of Health (NIH),
            National Human Genome Research Institute (NHGRI) Large-Scale
            Sequencing Research Network and the National Institute of Allergy
            and Infectious Diseases (NIAID) Microbial Genome Sequencing Centers
            Program.  The mission of the HMP is to generate resources to enable
            the characterization of the human microbiota, the microbial
            communities living within human bodies, and analyze the role of
            these organisms in human health and disease.
             The sequenced strain was obtained from ATCC.  The sequence
            generated included at least 10 fold coverage of Roche/454
            Lifesciences FLX fragment data and at least 8 fold clone coverage
            of Roche/454 Lifesciences paired-end data.
             The Roche/454 Lifesciences sequence was assembled using the
            Roche/454 Lifescience Newbler assembler.  The contigs from the
            Newbler assembly were aligned to the Illumina/Solexa data with
            mapping tools such as Mosaic and Crossmatch and these data were
            used for error correction for this version of the draft assembly.
            This draft assembly meets the HMP draft quality standards (more
            than 90% of the genome is included in contigs, more than 90% of a
            core set of bacterial genes are found with > 30% identity and > 30%
            length; more than 90% of the bases in the assembly have more than 5
            fold sequence coverage, the contig N50 length is greater than 5kb,
            the scaffold N50 length is greater than 20kb, and there is less
            than 1 gap per 5kb).
             This is a reference genome for the Human Microbiome Project.
            This project is co-owned with the Human Microbiome Project DACC.
            Genome Coverage: 32x
            Sequencing Technology: 454
            
            Annotation was added to the contigs in September 2009.
            Product names were updated in June 2013.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000160675.1-RS_2025_01_15
            Annotation Date                   :: 01/15/2025 00:59:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,883
            CDSs (total)                      :: 1,852
            Genes (coding)                    :: 1,825
            CDSs (with protein)               :: 1,825
            Genes (RNA)                       :: 31
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 9 of 27
            Pseudo Genes (incomplete)         :: 19 of 27
            Pseudo Genes (internal stop)      :: 5 of 27
            Pseudo Genes (multiple problems)  :: 5 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17603
                     /organism="Granulicatella adiacens ATCC 49175"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SCAFFOLD1"
                     /strain="ATCC 49175"
                     /isolation_source="biological product [ENVO:02000043]"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:49175"
                     /type_material="type strain of Streptococcus adjacens"
                     /db_xref="taxon:638301"
     gene            complement(1..1005)
                     /locus_tag="HMPREF0444_RS02850"
                     /old_locus_tag="HMPREF0444_0572"
     CDS             complement(1..1005)
                     /locus_tag="HMPREF0444_RS02850"
                     /old_locus_tag="HMPREF0444_0572"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="WP_005605764.1"
                     /translation="MRVITLGEILLRLSTADDVRLKSTKEFRACYGGAEANVGISLAH
                     FGHDVSVATVLPQDNPLNDAVSSRLRMNGIDTNLVATEYGRLGTYFLEQGNAGRASRV
                     TYDRLYSSISVLNELCWDLDEIFIDTDLLHISGILPALSKKWQAWAVEVVRKAKEYGC
                     YVSFDMNYRSKLWSYEEAYPCFQQILPYVDILSAGRLDAIHFLQVATAEEDVSLEEIY
                     RRVKEKYANIKVLYSTKRNVISTNHHELQGFYVGEDNNFVESKIYDIQPVIDRVGGGD
                     AFAAGILNGVLKGWDSQQIVDFGTASSVLKHTVFGDWNPFDENEVFEFMNQKSGQIVR
                     "
     gene            complement(1059..1688)
                     /locus_tag="HMPREF0444_RS02855"
                     /old_locus_tag="HMPREF0444_0573"
     CDS             complement(1059..1688)
                     /locus_tag="HMPREF0444_RS02855"
                     /old_locus_tag="HMPREF0444_0573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="WP_039848813.1"
                     /translation="MSSVLEQLKENYIFAVIRGKSSDEAIEISKHSVLGGIKNIEVTY
                     TTPEASKAITELKALYAKKSEVVIGAGTIMTVELAKEAIAAGAEFLVSPHYDAHIQKV
                     AHEAHIDYFPGCATTTEIVQAMNGGAKIIKLFPGGVLGPSFIKDIHGPIPNVNLMPSG
                     GVSLANIKEWKEKGAVAVGVGSALGAKVATEGYESVTRIAKEFVNALVD"
     gene            complement(1743..3602)
                     /locus_tag="HMPREF0444_RS02860"
                     /old_locus_tag="HMPREF0444_0574"
     CDS             complement(1743..3602)
                     /locus_tag="HMPREF0444_RS02860"
                     /old_locus_tag="HMPREF0444_0574"
                     /inference="COORDINATES: protein motif:HMM:NF028199.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="WP_005605768.1"
                     /translation="MFTNLETYQSRFWLKEEFLKKIGKSSSGFLLELFSRVDALVDQD
                     LVYYDHQMDMETCFEKYSFHEFKWHQTPNGDMEWIFMLNRQGYLVDLAVASYVTKDEK
                     YLKKWKEIVFDFILENEEEISHPEASWRVLDAGIRLMNWVKSLTYLPLSEFSEVERKQ
                     IQEAMSLHVNYIRTHLQEKHLLSNWGVLAVSGVLSFVYFFDEQPELYLWAKETLLNQA
                     SLQFTKKGLHWEQSPLYHHQVVMCFAYLYQIACYMGYEDQHLWKEVLIEPVRASYYLS
                     NSNHMLLALNDSDTVDFSWVHSYYHLLGFESECPEYTMGVLYSGRAFDKKHKVNFENW
                     FYDEVGGIAVVKSSDIYFSLFCGLHGSSHGHASHGAISLEMDGAPFITHPGRYTYTEC
                     EKRVHFKSEQMQNTVLVEGGHYPSIESSWGYHWVAKPLSMQMKKEDELYYFEAQWSIP
                     MKDNETSSRAYVSRKMILDTQAKLLLVIDEEECGHALTQNFIIPTKHSNDVYSRFWTN
                     GEMVTEAIQYSKRYNSLDEGIQLKIRNKKDPIFTCIALEPYSVEKESIPRLDCPGEER
                     GTKIKCKTKSGEITIYYSPEDIHNGLKYLKDSNGNIVYGRMVAEKNNQIKRFR"
     gene            complement(3612..4427)
                     /locus_tag="HMPREF0444_RS02865"
                     /old_locus_tag="HMPREF0444_0575"
     CDS             complement(3612..4427)
                     /locus_tag="HMPREF0444_RS02865"
                     /old_locus_tag="HMPREF0444_0575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359831.1"
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]; GO:0016310 -
                     phosphorylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="WP_005605769.1"
                     /translation="MESKQNKLTKRDYFRTSLRSYILQNGFNYNTYQGVSYLNVILPG
                     LKKIYKDDPEKLKETAKANLEFYNTSPHLVPFISNLQLAMYEDGQSIDSVRSIKMALM
                     GPLAGIGDSIAQFGLAPLFSTIFAGLALDGLGFAPMGYWLAMLLSMFAIKVFMGYLGY
                     RLGTNAIKTLSAQISKISEAANIVGVTVISALAASFVKAKIAIQYATTVSSGEEQIVS
                     IQKILDKMMPRLLPVLITVLVFYLLKKKKWNTYQLLILLFAIGILASVLGILA"
     gene            complement(4417..5217)
                     /locus_tag="HMPREF0444_RS02870"
                     /old_locus_tag="HMPREF0444_0576"
     CDS             complement(4417..5217)
                     /locus_tag="HMPREF0444_RS02870"
                     /old_locus_tag="HMPREF0444_0576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="WP_005605771.1"
                     /translation="MEISLMQGILIGLVTAFCYSGMLLGIYTNRAIVMAFFVGLILGD
                     IPTALTFGALAELAYMGFGVGAGGTVPPNPVGPGIVGTIMVITLKDQGITPENALALS
                     FPFAVLFQFVTTFLYTFFAGNPKMAEKAIENGHFGKFKLIAHSTYIGLAVAGFIIGIL
                     SALSRPALEAFVNALPKWLISGFGVAGGLIPAIGFAMILSVMLKKELTPYVILGYICV
                     AYLELPTMAVALVGSVFALIAYYQNSNKQEVVVETVQTSEVEDFSNGI"
     gene            complement(5232..5732)
                     /locus_tag="HMPREF0444_RS02875"
                     /old_locus_tag="HMPREF0444_0577"
     CDS             complement(5232..5732)
                     /locus_tag="HMPREF0444_RS02875"
                     /old_locus_tag="HMPREF0444_0577"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605773.1"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_005605773.1"
                     /translation="MENKPNIKMVRIDERLIHGQGQLWIKSLGVNLAICANDAVSTDE
                     LQQTLMTTVLPKEVNVRFWTIQRTAEIIWKAAPHQTIFVVVSSPADALRLCELGFPME
                     NVNVGNIHAAPGKDKVSQFIYLGEEDKQALRTMKEKFNVQFNTKTSPVTNDGAQTLEK
                     LLEMIS"
     gene            complement(5756..>5934)
                     /locus_tag="HMPREF0444_RS09880"
                     /old_locus_tag="HMPREF0444_0578"
                     /pseudo
     CDS             complement(5756..>5934)
                     /locus_tag="HMPREF0444_RS09880"
                     /old_locus_tag="HMPREF0444_0578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="incomplete; too short partial abutting assembly
                     gap; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            complement(<5985..6987)
                     /locus_tag="HMPREF0444_RS02880"
                     /old_locus_tag="HMPREF0444_0579"
                     /pseudo
     CDS             complement(<5985..6987)
                     /locus_tag="HMPREF0444_RS02880"
                     /old_locus_tag="HMPREF0444_0579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611817.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
     gene            complement(7052..10366)
                     /locus_tag="HMPREF0444_RS02885"
                     /old_locus_tag="HMPREF0444_0580"
     CDS             complement(7052..10366)
                     /locus_tag="HMPREF0444_RS02885"
                     /old_locus_tag="HMPREF0444_0580"
                     /inference="COORDINATES: protein motif:HMM:NF019735.5"
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="WP_169304263.1"
                     /translation="MMKKVWSSCLVLAFALVSSLGVSQGNLVYAEDHKETQAEQTVNL
                     VKNGTFDSTLDTGEKWTGKSAVDWNTPWIAKGEKGKYKIQITDEANLLMEAESEMRAV
                     VGQDIPVEPNQKYTFSVRIKTEKLDSKIGARVRIMSYDSNNKQLSPLWYSKSLVGDND
                     WTTITEEFVAGSEATKIRVELFFETGKGKVQFDDVSLTKKKEKKETQPQKTKEIDFDD
                     SVTLTTDKRWVLDSKYTYKIKDTSVATIENGAIVPKKAGSTELVVSAPGSKDKVVPLK
                     VLEPNATYENLLKKWDAMISGNDFYSTESSYMKEIFNINEKEVEGYLSSFLANDEKKI
                     WDAVGDYSKSANLTKTYRRVEQIAKSVTNKNSKYYNDDKVIQVVKHSMKFMYDHYYNE
                     NTESKGNWWDYEIGVPRAINNILTIMNRYFSKEEIAKYLKPVSKMVPDPSKIMVSQNR
                     GKTAVGGNLSDLGKVKIIEALLLEDDAKLERSIQAVSNVLALVSEGEGFYYDGSYVDH
                     TNIAYTGAYGNVLIDGFSQLLPVIQASPYKISNDKLNVLYHWIHQGFLPLIYKGGLMD
                     MTRGRSLSRKVQNDHYAAVEVLRGILRIAEASDQQEKAKLQGLVKDIVSSDDFYDTFK
                     SLKSFYDVHLFESLMSNQMVEATPRDTYLKLYQAMDKVAYYNKEREFAFGISMYSNKI
                     QNYEFMNKENAKGWYTSDGASYLYNDDLSYYSDDYWATVDPYKLAGITETNEARAKGS
                     GMTTMDHSFVGSTALGNKYGTVAMDFQNWNKTLTAKKSWFILGDRIVFLGSDIKNSTD
                     NEAYTVVDNRKVQRESRNRSASKNDYSVVADGKEVPVSDADTKMQVKDLLLKSKNTQM
                     NIGYKFLEPTTVNVKKENRKGTWKDINEGQSDEEVENSFISIVQPHDATNNKYAYVLY
                     PNRSAKEFEEEKNNDDIQVVENSEKTQAVFDKTNQIYGVVKYDDSELTLEDGLVLKEK
                     GIYTIKKEGNKLDIAFLNPEDPSAGLPALNTDKYELQNSTEPTVENKIRYYSYLVKKQ
                     TEEETTQEQTQEQTQQSSSESTTEIRNTEDSKDSNTTKEETLPATGAKNFEVYTLLGI
                     LLLTISLIFSKKRLQK"
     gene            complement(10500..11189)
                     /locus_tag="HMPREF0444_RS02890"
                     /old_locus_tag="HMPREF0444_0581"
     CDS             complement(10500..11189)
                     /locus_tag="HMPREF0444_RS02890"
                     /old_locus_tag="HMPREF0444_0581"
                     /inference="COORDINATES: protein motif:HMM:NF012610.5"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_005605781.1"
                     /translation="MLPKYEIIKQNILKEIDEGKFKPGSKIYSEGDLKKIYQVSNTTV
                     VKALNDLVAEGYLIRRQGEGTFVRKNMLHRKVSFSERLTKKFNPKKNSERVETSIQIC
                     EDKRVLSQFEGKAPLYEIVQRAMIDDTLWKIQKRFVQTTLLSEEAVRHITDGGSVSKE
                     LGIENNLVNMPMSMTIEFKTVDILETNELQTNSVVIVKKTIFNNTTKEVIEYSESFIH
                     PDFYEVNLIAE"
     gene            complement(11291..12088)
                     /locus_tag="HMPREF0444_RS02895"
                     /old_locus_tag="HMPREF0444_0582"
     CDS             complement(11291..12088)
                     /locus_tag="HMPREF0444_RS02895"
                     /old_locus_tag="HMPREF0444_0582"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605782.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="WP_005605782.1"
                     /translation="MTNDMFSLKGKVALVTGAVYGIGFAIAEALASAGATIVFNDRNE
                     SGVEKGLANYAEKGIKAYGYVCDVTDEPGVQEMVKKIEAEVGVIDILVNNAGIIKRIP
                     MHEMAAEDFRQVIDVDLNAPFIVSKAVIPSMIKKGGGKIINICSMMSELGRETVSAYA
                     AAKGGLKMLTRNIASEYGCYNIQCNGIGPGYIATPQTAPLREIQPDGERHPFDKFIIA
                     KTPAERWGEAEDLKGPAIFLASGASDFVNGHILYVDGGILAYIGKQP"
     gene            complement(12104..12934)
                     /gene="kduI"
                     /locus_tag="HMPREF0444_RS02900"
                     /old_locus_tag="HMPREF0444_0583"
     CDS             complement(12104..12934)
                     /gene="kduI"
                     /locus_tag="HMPREF0444_RS02900"
                     /old_locus_tag="HMPREF0444_0583"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875393.1"
                     /GO_function="GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="WP_005605784.1"
                     /translation="METRYTHSPEDIRHYSTEQLRKEFLVEKVFEPNQVKLTYTHNDR
                     MIFGGVMPVEGKLTIELSTELGVDYFLQRREMGVINIGGPGVIEIDGVSENMVKQDGY
                     YIGKETKEIAFSSVSAEDPAKFYIVSVPAHHKYPNKKLSIEKIVPLCMGESNTLNQRK
                     IYQYVHPNQCESCQLQLGYTVLEPGSSWNTMPCHTHERRMETYLYFDMEPDTRVFHFM
                     GTPQETKHLVVGNEEACISPSWSIHSGVGTSNYTFIWGMAGENITYTDMDMVPMQDLK
                     "
     gene            complement(13363..13884)
                     /locus_tag="HMPREF0444_RS02905"
                     /old_locus_tag="HMPREF0444_0584"
     CDS             complement(13363..13884)
                     /locus_tag="HMPREF0444_RS02905"
                     /old_locus_tag="HMPREF0444_0584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="WP_005605786.1"
                     /translation="MSVFQKEFKVETKEKAVSYVDITQSVKDTILESGIQSGTCTVVT
                     AHTTCSIFFEEDTHDRDEDGVDFLQLDLNRILESIVPPHINAQSYKYPGEAHYKEVES
                     WPNPEEWLPNGNRSYLWNGDAHIKATIIGASETLAVVDGQLGIGKTGYVYFADFDKTR
                     KRTRKYRVIVLGE"
     gene            complement(13887..14576)
                     /locus_tag="HMPREF0444_RS02910"
                     /old_locus_tag="HMPREF0444_0585"
     CDS             complement(13887..14576)
                     /locus_tag="HMPREF0444_RS02910"
                     /old_locus_tag="HMPREF0444_0585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="WP_005605788.1"
                     /translation="MKIVKSKFFIFNPKSYLFGDELLELAKEADHLAEVYPEISIFVT
                     CPYADISRVASETKNIIVTAQHLDGIDCGRGMGAVLPASLYNAGARATFLNHAEHPLT
                     TSQVVASVNKAKELGMITVLCADSIQEARMLAMLEPTIMLCEPTELIGTGQTSDDSYI
                     VKTNEQVKSVYPNILMMQGAGIMNEKDVYRTIKLGAEGTGCTSGIVKAENPKEMLRLM
                     VEAIDQVMNEG"
     gene            complement(14592..15416)
                     /locus_tag="HMPREF0444_RS02915"
                     /old_locus_tag="HMPREF0444_0586"
     CDS             complement(14592..15416)
                     /locus_tag="HMPREF0444_RS02915"
                     /old_locus_tag="HMPREF0444_0586"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="WP_005605791.1"
                     /translation="MKARMRDVLGKAKKEHYAVPAANMIDWNCAKAYSETSEKLELPL
                     ILAFAQVHSQYLSLEDAAAIGRYFQEVTNTPVVLHLDHGQDFEFIKRAIELGFNSVMI
                     DASLDTFEENVRKSKEVVEYAHQFGVDVEAEIGFVGANAASNNEEVKSIYTSVEDAKK
                     FVEETNVDSLAVSIGTSHGLYKGTPKLNFERLEELNEALTIPLVLHGGSGSGDDNLNR
                     CATHGISKINIYSDFIYSGAKAVADGYVENYIDIVNLSRKGMENTLEHYYKVFETK"
     gene            complement(15419..15703)
                     /locus_tag="HMPREF0444_RS02920"
                     /old_locus_tag="HMPREF0444_0587"
     CDS             complement(15419..15703)
                     /locus_tag="HMPREF0444_RS02920"
                     /old_locus_tag="HMPREF0444_0587"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090362.1"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_005605792.1"
                     /translation="MKKLLVMCGSGIATSTVVVGKVKQWLKDNGYEDKVQVFQSKVSE
                     EVNRLDEYDVVISTTVVPDSVKDKVIMGLPLLTGMGIDSLWNELKQALEA"
     gene            complement(15715..17040)
                     /locus_tag="HMPREF0444_RS02925"
                     /old_locus_tag="HMPREF0444_0588"
     CDS             complement(15715..17040)
                     /locus_tag="HMPREF0444_RS02925"
                     /old_locus_tag="HMPREF0444_0588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="WP_005605794.1"
                     /translation="MLELFQKFIDLGAIVVLPILIFIFGMALGTKPKKAFVSGIIVGV
                     GFVGLNMVVDLLGGSLGPAAQAMVERFGLNLTTIDVGWPAAAAISYGTLLGSLAIPIG
                     IGINILLLFLGLTKTLMVDMWNFWHAAFVASLVYAVTQDFTLGLYATATYLVMIYLLA
                     DLIAPAIKKFYGFPNITFPHGTSAPGFLVAIPLNWLFDRIPGFNKIEANPESIQKKFG
                     VFGESTVLGLLIGIAMGLLAGYDAKGVLQLGVKMSAVMVLMPRMVSLLMEGLTPISEA
                     ANEFVQKRFPGREVNIGMDSALSVGHPAVLSSSLLLVPITILLAVILPGNQTLPFGDL
                     ATIPFVVCLMAAVFKGNIVRTVVGGAIYMTSIFYITSWVAPLVTQAAKAANFDLGGNT
                     SITALAEGGLWPTWLFVFAGQTAPWIILTVVLVGSLAGLFYVNKVRVAK"
     gene            complement(17139..17603)
                     /locus_tag="HMPREF0444_RS02930"
                     /old_locus_tag="HMPREF0444_0589"
     CDS             complement(17139..17603)
                     /locus_tag="HMPREF0444_RS02930"
                     /old_locus_tag="HMPREF0444_0589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605796.1"
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]; GO:0016310 -
                     phosphorylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="WP_005605796.1"
                     /translation="MLNEEVFEMNVPNMKTKEEALSYLADKLISKGIVKESYKQAIID
                     REHVFPTGLQFEAYGIAIPHTDIEHVNKEQIALMTLKDAVSFYQMGTNDVEVPVRVIF
                     MLALKEAHSQLTMLQQLMEVLQDKDIMERILSMDENTPNSEIKEVLALKNIK"
     gene            complement(17593..>17603)
                     /locus_tag="HMPREF0444_RS02935"
                     /old_locus_tag="HMPREF0444_0590"
     CDS             complement(17593..>17603)
                     /locus_tag="HMPREF0444_RS02935"
                     /old_locus_tag="HMPREF0444_0590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605798.1"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]; GO:0006355
                     - regulation of DNA-templated transcription [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="WP_005605798.1"
                     /translation="MNARQIFLLEFLLKQNEYLSANQLSEKYGVSTKTVYQDIDKINY
                     FLEEGALNSRIIKVPRKGIKLSADEERKRIHAFLLENKFESGAENYSPEYRELEIIKR
                     LFINQDKLDIYEFAEEMYVTESTVHRDIDKLENNLGEFDLKIRIKHDRFYVDGDEWSI
                     RKALHSYITQNPTIRSEEGLEGFFTKGDVEKCREAIDRLSQKYHHQFSDDYSWLLLTE
                     CLVFKKRTDQNNYLTKRTSNLINDLNHLEVYFFSGELLENIIDKPFSEISPYEIEAMA
                     YSLLAYGFSIQSADYIQNIEEQVNELIEKVSSLLSLDLTKDKHLKLMLSNHISKMIFR
                     LRNQIYITNPAIEEIKKQYSSLFNVIWIAIRDLGKYYEINIPNEELAFIVIHFQLAIE
                     KILKPLNVGVICQNGIATSELIMSKLHKIFDADAKISNISVREIDFYDLSNIDLIIST
                     ISLPDITIPVIEVSPILTKGEIESIRSFYTDHMTDNYTLMRTSLDGRKFNMESLQTLI
                     KTPKLVHVSVKNKKECIEKMIDECDLSNRRIQGFKESILERETLGSTSVYTGVALPHC
                     DPRFVNQSELIFMTLDKPINWGKNNVKLIILIAVAENDIPIFKDSLIALYSVIENQEL
                     MNELVFMNNGDEIKERLLKEVSQNAK"
ORIGIN      
        1 ttaacgaaca atctgtcctg atttttggtt cataaattca aagacttcat tttcgtcgaa
       61 cggattccag tcgccaaaaa ccgtatgttt taataccgaa cttgctgttc caaaatcgac
      121 aatttgttgg ctatcccaac cttttagaac tccgtttaga atcccagcgg caaaagcatc
      181 accaccgcca actcgatcaa ttacaggttg aatatcatag atttttgact ctacaaagtt
      241 attatcttct cctacataaa aaccttgtaa ctcatggtga ttggttgaaa tgacatttct
      301 ttttgtggaa tataacactt tgatattcgc atatttttct ttcacgcgac gatagatttc
      361 ttcaagagat acgtcttctt cagctgttgc aacttggaga aaatgaatcg catcgagtct
      421 ccctgcagat aaaatatcta cataaggcaa gatttgttgg aaacaaggat aggcttcctc
      481 ataactccac aacttactac gatagttcat atcaaaactc acatagcatc catattcttt
      541 cgctttacgg acgacttcaa ccgcccaagc ttgccacttc ttagaaagag ctggtagaat
      601 tcctgaaata tgaagcaaat cagtatcaat aaagatttcg tctagatccc agcatagttc
      661 atttaaaacg gagatactag aatataagcg atcgtatgta acacgagaag cacgtcctgc
      721 atttccttgt tctaaaaagt aagttcctaa tcttccatat tctgtcgcta ctagattcgt
      781 atcaattcca ttcattcgta atcttgaact tacagcatcg tttaaaggat tatcttgcgg
      841 tagaaccgtt gccacagaaa cgtcatggcc aaagtgcgct aaggaaatcc ctacatttgc
      901 ttcagctcca ccatagcagg ctctgaactc ttttgtactt tttaaccgta catcatctgc
      961 ggtcgataat cttaacaaaa tttcacctaa agtgatgact ctcatcttgt ctcctctttt
     1021 cttaaataga attttttaga aggatgaatt acctccaatt aatcaaccaa agcattcaca
     1081 aattctttag caatgcgtgt gacactttca tacccttctg ttgcaacttt cgcaccaaga
     1141 gcacttccaa ctcctactgc tactgcgcct ttttctttcc actctttaat attggctaga
     1201 gaaactccac ctgaaggcat taaatttaca tttggaattg ggccatgaat atctttaatg
     1261 aacgaaggcc ctagaactcc acccgggaat aatttaatga tttttgctcc cccattcatc
     1321 gcttgaacga tttctgttgt tgttgcgcat cccgggaagt aatcgatatg agcttcatga
     1381 gcaacctttt gaatatgtgc atcatagtga ggacttacta aaaattccgc accagcagca
     1441 attgcttctt tagccaactc tactgtcata atcgttccag caccaatgac aacttctgat
     1501 ttttttgcat aaagagcttt caattcagta attgctttag aagcctcagg tgttgtataa
     1561 gtcacttcaa tatttttaat tccacctaat acagaatgtt ttgaaatttc aatcgcttca
     1621 tctgatgatt tcccgcggat aactgcaaaa atatagtttt cttttaattg ttctaataca
     1681 cttgacatgt cctttctctc ctttataaat ctggctttgt ttgtatacat agtctagtct
     1741 ttttatctga atctttttat ctgattattt ttctcagcaa ccattctccc ataaacgata
     1801 tttccatttg aatcttttag atatttcagt ccattatgaa tatcttccgg agaataatag
     1861 atcgttattt ctccactctt ggttttacat tttatttttg tccctctttc ttctcctgga
     1921 caatctaggc gagggatgct ctccttctcc actgaataag gttccaatgc aatacatgta
     1981 aaaatgggat cttttttatt tctgattttt aattgaatgc cttcatccaa cgaattgtat
     2041 cgtttagaat attggatagc ctctgtaacc atctctccat tcgtccagaa cctggaatac
     2101 acatcattcg aatgtttcgt tgggatgata aagttttggg ttaaagcatg tccacattct
     2161 tcttcatcaa tcaccaataa aagtttagct tgcgtatcca aaatcatttt tcgactaaca
     2221 taagctctac tagaagtttc attatctttc attggaatac tccattgtgc ttcaaaataa
     2281 tagagttcgt cctccttttt catctgcatt gataaaggtt tggctaccca atgatatccc
     2341 catgagcttt cgatagaagg ataatgaccg ccttctacta ggaccgtatt ttgcatctgt
     2401 tctgatttaa agtgaactcg tttttcacat tctgtataag tatatctccc tggatgtgtt
     2461 ataaaaggag ctccatccat ttctagactg attgctccgt gggatgcatg accatgtgag
     2521 cttccatgaa ggccacaaaa cagactgaag taaatatctg aactttttac aaccgctatt
     2581 ccgcctactt catcataaaa ccagttttcg aaattcactt tatgtttttt atcaaacgct
     2641 ctccctgaat ataaaactcc cattgtatac tctggacatt cactctcaaa ccccagcaaa
     2701 tgatagtagg aatggaccca agaaaaatca accgtatcgc tgtcatttaa cgctaaaagc
     2761 atatgattag aattcgataa ataataactc gcacgaacag gttcgattaa aacttccttc
     2821 caaaggtgtt gatcctcata tcccatataa caagcaatct gatataaata tgcaaaacac
     2881 atgacgactt gatgatgata cagtggactt tgttcccaat gaagtccctt ttttgtaaac
     2941 tgaagagacg cttgatttaa aagtgtttct ttagcccaca aatacagctc aggttgttca
     3001 tcaaagaaat atacgaaaga aagaacacca ctgacggcaa gaactcccca gttactaaga
     3061 agatgcttct cttgtaaatg agtacgaata taattaacat gcaaagacat cgcttcctga
     3121 atttgctttc tttctacctc actaaactct gatagtggaa gataggttaa acttttcacc
     3181 caattcatta aacgaatgcc agcatccaac actctccatg aagcttctgg gtgagaaatc
     3241 tcctcttcat tttccaaaat aaaatcaaaa acgatttcct tccacttttt caaatacttt
     3301 tcatctttgg ttacataaga cgctactgct aaatcaacaa gatatccttg tcgatttaac
     3361 atgaagatcc attccatatc tccatttggc gtttggtgcc atttaaactc atgaaatgaa
     3421 tatttttcaa aacacgtttc catatccatt tgatgatcat agtagactaa atcctggtca
     3481 actaaagcat ctactctaga aaaaagttca agcagaaacc cacttgaact tttccctatc
     3541 ttttttagaa attcttcttt taaccaaaat ctagattgat atgtctctag attcgtaaac
     3601 atctcatcac cttatgctaa aattcctaga acagaagcta agataccaat tgcgaataat
     3661 aggattaata attgataagt gttccatttt ttcttcttca atagatagaa cactaatact
     3721 gtaattaata ctggtaataa tcttggcatc attttatcta agattttttg aatagaaacg
     3781 atttgttctt caccagaact tacagttgtc gcatattgaa tggcaatttt agcttttaca
     3841 aatgaggctg ctaatgcaga gataactgtt accccaacaa tattggctgc ttcagagatt
     3901 ttgctaattt gagcgcttaa agttttaata gcatttgtcc ctaaacgata tccaagatat
     3961 cccatgaata ctttgatagc aaacatactt aataacattg ccaaccaata tcccattgga
     4021 gcaaatccta gaccatctag agctaaacct gcaaagattg tagagaacaa tggcgctaaa
     4081 ccgaattgtg cgatagaatc tccaatccct gctaaaggac ccataagagc cattttaata
     4141 ctacgtacag aatcaatact ttgaccatct tcgtacatcg ctaattgcaa gttcgaaata
     4201 aacggtacta aatgtggact tgtgttatag aactccaagt ttgctttagc agtttctttt
     4261 aatttttctg gatcatcttt ataaatcttc tttaagccag gtaagataac gtttaagtag
     4321 ctgacccctt gataagtatt atagttgaat ccattttgta agatgtatga tcttaatgat
     4381 gttctaaaat aatcacgttt cgttaattta ttttgtttag attccattgc taaaatcctc
     4441 cacttcgcta gtttgaactg tttccacaac tacttcttgt ttattagaat tttggtagta
     4501 agcaattaat gcaaatactg aaccaactaa tgctacagcc attgttggta attctaagta
     4561 agccacgcag atataaccta ggattacgta tggagttaat tcttttttca acattacaga
     4621 taaaatcatt gcaaaaccga ttgctggaat taacccacca gctacaccga aaccactaat
     4681 caaccatttt ggtaaagcat taacaaatgc ttctaatgct ggacggctta gtgcagataa
     4741 gataccaata atgaaaccag caactgctaa accgatataa gttgagtgtg cgattaattt
     4801 aaatttacca aagtgaccat tttcgatcgc tttttcagcc atttttggat taccagcgaa
     4861 gaatgtgtat aagaatgttg taacgaattg gaataatacc gcaaatggga atgaaagtgc
     4921 taatgcattt tcaggtgtaa tcccttgatc ttttaatgta attaccatga ttgtacctac
     4981 gataccaggt cctactgggt taggaggaac tgtaccacca gcacctaccc cgaatcccat
     5041 ataagctaat tcagctaaag ctccgaatgt taatgctgta ggaatgtctc ctaaaattaa
     5101 ccctacaaaa aacgccataa caattgctct gtttgtataa attcctagta acatccctga
     5161 ataacagaat gctgtcacta gaccaatcaa aataccttgc attaatgaaa tttccattgt
     5221 tctccctctt ttcagctaat catttctaat aatttttcta aagtttgtgc tccgtcattc
     5281 gtaactggag aagtttttgt attgaattga acattgaatt tttctttcat tgttcgaagt
     5341 gcttgtttat cttcttctcc tagataaatg aattgtgata ctttatcctt tccaggagcc
     5401 gcgtgaatgt tacccacatt cacattttcc attggaaatc ctagttcaca taagcgaagt
     5461 gcatccgctg gactagatac cactacaaaa attgtttggt gtggggcagc tttccaaata
     5521 atttcggcag ttctttgaat cgtccaaaaa cgtacattca cttcttttgg caatacagtt
     5581 gtcattaatg tttgttgtaa ttcatccgtt gacactgcgt catttgcaca aatggctaag
     5641 ttgactccta aactcttaat ccataattgt ccttgaccat ggataagtct ttcgtcaatt
     5701 ctgaccattt taatgtttgg tttattttcc atattctcat ccctttcttt tctttttacc
     5761 agtacatttc ccagtctttg tttttacgca taagcgcttc tagatagaag taatcacccc
     5821 aaatatttcc ttcgttaacc cccttccctg aatgccatga atatactcct tcatttaata
     5881 aggcaacgat agtagatcct tttttatccg tatatttggt gattaagtta tatannnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaattc tgtctgccca
     6001 tttttcatac tctttaaccg cttcattatt tggaagctct ttagccattt gctggatacc
     6061 gcaaactgca atggcagctg cagaagtatc tcttggttga cctgaagtat catcaaagat
     6121 taagtcccaa taacatacaa agtcttccgg cacacgattt aagaagtagt ttgttaccgc
     6181 ttggaaatat tcaacattag tttcggatgg agcatacgca ttgcaaagag caattccata
     6241 aattaaccat gcttgccctc ttgcccatgc agagtcatct gaataccctt gtcttgtttt
     6301 tccatatgca ggctctcctg tttctggatc aaagtagaaa gtatggaaag ctgaagcatc
     6361 ttgacgaata gcgtttttca aagttgtctg gaagtgagct ttagccattt cttcatattt
     6421 actatctcca gtttcattac ttgcccagaa tagaagagga atatttaata aacaatccac
     6481 aattaagcgg tagttatcac gtgctcctaa ttctccccaa gcttgaataa agtttccttt
     6541 ttcttgatat cttgaagcga gttggtccgc agctttaatg gccgcttctt ttgctttctc
     6601 atttcctgtt agcttataag cgctgacaca agaaggcgta tataggaagc ctaagtcgtg
     6661 gtgattaact tcgattaatc gttcaattcg ttcaatgaag gaatccacat tcttttcggc
     6721 agctacttta tatttctctt ccccagtaaa ttcataagcg agccacaaca ttccagtcca
     6781 aaaaccattg gtccattcga tatttcccat cggtttatag tcattatcga atgttgctgg
     6841 cgttgggtat tgttccccca actgctctaa gttactatca attttttcta aacaaacttt
     6901 gatcgcttca tctactaaag caggagttaa gacctccgcc tcattcggtt gatacacttt
     6961 caaaagttcg atatttttat caatcattct tgcacctctc tataaggcaa cttaacacgt
     7021 taactcgtta agttcattgt accctatttt tttatttttg taaacgtttt tttgaaaaaa
     7081 taagtgaaat tgttaataat aaaattccaa gtaacgtata cacttcaaaa ttcttagccc
     7141 ctgtggcagg taaagtctct tcttttgttg tattggagtc ttttgaatct tctgtgtttc
     7201 taatttcagt agttgattca cttgaacttt gttgtgtttg ctcttgtgtt tgttcctgag
     7261 ttgtttcttc ttctgtttgt tttttcacta aatacgagta atatctaatc ttgttttcaa
     7321 cagtaggttc tgttgaattt tgtaattcgt atttgtctgt gttgagtgct ggtaggcctg
     7381 ctgaaggatc ttctggattt aagaatgcaa tatctagttt attgccctct ttcttaatgg
     7441 tataaatccc tttttctttt aatacgagac catcttctaa cgtcaactca gagtcatcat
     7501 attttacaac tccgtaaatt tgattcgtct tatcaaagac tgcttgcgtt ttttcagaat
     7561 tttctacaac ttgaatgtca tcgttgttct tttcctcttc aaattcttta gctgaacgat
     7621 ttggatagag gacatacgca tatttattat tcgtagcatc atgtggttga acaattgaaa
     7681 tgaagctatt ttctacttct tcatcacttt gtccttcatt aatatccttc caagttcctt
     7741 tacggttttc ttttttcaca ttaacagttg tcggttctag gaatttatat ccaatattca
     7801 tttgcgtatt tttagatttt aagagtaaat ctttcacttg cattttggta tcagcgtctg
     7861 aaacaggaac ttcttttccg tctgcaacca cactataatc attcttcgaa gcactgcgat
     7921 ttcttgattc tctttgaact ttacggttat cgactactgt ataagcttca ttatccgtac
     7981 tgttcttaat atccgaaccg aggaatacga ttcgatcgcc tagaataaac catgattttt
     8041 tggcggttag tgttttattc cagttttgga agtccatcgc aaccgttccg tatttattac
     8101 ctagcgctgt acttccaaca aatgaatgat ccattgttgt catcccagac ccttttgctc
     8161 tagcttcgtt cgtttcagta atccctgcta gtttataagg atcaacggtt gcccaataat
     8221 catcactata ataacttaaa tcatcattgt agagataaga ggcaccatca cttgtatacc
     8281 aacctttggc attttcttta ttcatgaatt cgtagttttg aatcttgttc gaatacatgc
     8341 taattccgaa agcaaattca cgttctttat tgtagtaagc cactttgtcc atcgcttgat
     8401 agagtttgag atacgtatct cttggagtcg cctctaccat ctgattagac attagacttt
     8461 caaagagatg aacatcgtag aaagacttca atgatttaaa ggtatcgtag aaatcatcgg
     8521 aagaaacgat atctttcact agtccttgaa gtttcgcttt ttcttgttga tcacttgctt
     8581 cggcaattct taaaatacct cttaaaactt ccactgcagc atagtgatca ttttgtactt
     8641 ttctgctaag tgagcgacct cttgtcatat ccatcaaccc acccttataa atcaaaggta
     8701 agaatccttg atgaatccag tgataaagaa catttaattt gtcattacta atcttataag
     8761 gacttgcttg aataacaggg agtaattgag aaaatccatc aattaataca tttccgtatg
     8821 cccctgtata agcaatattt gtatgatcta cataagaacc atcatagtag aacccttccc
     8881 cctcagaaac taatgctaat acattactta cggcttgaat tgagcgttct aatttcgcgt
     8941 catcttcaag gagtagcgct tcaatgattt taacttttcc caaatctgaa aggttcccac
     9001 caacagctgt tttaccacgg ttttggctga ccatgatttt agatgggtct ggaaccattt
     9061 tgctcactgg ttttaagtat ttcgcaattt cttctttact gaaataacga ttcataatcg
     9121 ttaggatatt gttgatggca cgaggaactc caatttcata atcccaccaa tttcctttag
     9181 attcagtatt ttcattgtag taatggtcat acatgaattt catgctatgt ttgactactt
     9241 gaataacttt gtcgtcgtta tagtattttg agtttttatt cgtaactgat tttgcaattt
     9301 gttccacacg tctatacgtc ttcgttaaat tggctgattt agagtaatcc ccaactgcat
     9361 cccaaatttt cttttcatca tttgctaaga aactagatag atacccttct acttcttttt
     9421 cattaatgtt aaagatttct ttcatataac tactctctgt cgagtaaaag tcattcccag
     9481 aaatcatggc atcccatttt tttaacaagt tttcataggt tgcatttggt tcaagcactt
     9541 tcagtggaac tactttatcc ttacttcctg gagcgcttac aactaattca gtacttccag
     9601 cctttttagg aacaattgct ccgttttcaa tggtagcaac agaagtgtct ttaatcttgt
     9661 acgtatattt ggaatcgagc acccatcgtt tgtcagtcgt taaagtaact gaatcatcaa
     9721 aatcaatttc ttttgtcttt tgcggttgag tctctttctt ctcctttttt ttggtcaagc
     9781 tcacatcatc aaattgaact tttccttttc ctgtttcaaa gaataactcc actcggattt
     9841 ttgtcgcttc acttcctgcg acaaactcct ccgtaatcgt cgtccaatcg ttatctccga
     9901 ctaaactctt gctataccaa agtggtgaaa gctgtttgtt attgctgtca taagacataa
     9961 tgcgaactct agcaccaatt tttgagtcta atttttctgt tttgattctc actgaaaaag
    10021 tatacttttg attcggttca actggaatat cttgtcctac taccgctctc atttcagatt
    10081 cagcttccat taaaagattc gcttcatctg taatttgaat tttatacttt cctttttctc
    10141 ctttagcaat ccaaggagta ttccaatcaa ctgctgattt tccagtccat ttttcccctg
    10201 tatcgagggt cgaatcaaac gttccatttt ttacaagatt gactgtttgt tctgcctgag
    10261 tttccttatg atcctcagca tatacaagat ttccctgaga gactcccaga gacgaaacca
    10321 aagcaaaagc taaaactaaa cagctcgacc aaactttttt catcatgacc atccttccgt
    10381 ttttaactta acgtgttaac cctataaatg aattctacca ctccttttag aatttgtaaa
    10441 cggtttattt aaaaaaatta aaaaaaataa gaatccccca cattaggagg attcttattt
    10501 tattcagcaa tcaaattaac ttcataaaaa tcaggatgga taaagctttc cgagtattca
    10561 atcacttctt tggttgtatt gttaaagatt gttttcttta caatcactac cgaattggtt
    10621 tggagctcgt ttgtttcaag aatatccact gtcttaaatt caatcgtcat gctcataggc
    10681 atatttacta agttattttc aatacccaac tctttagaga cacttccacc atcagtaata
    10741 tgacgaactg cctcttcact taataaagtc gtttgaacaa aacgcttttg aattttccat
    10801 aaagtatcgt caatcatcgc tctttgaacg atttcatata aaggagcttt cccttcaaat
    10861 tgacttaaaa cgcgtttatc ttcacagatt tgtatcgaag tctctactcg ttccgaattc
    10921 tttttcggat tgaatttctt cgttagtctt tcactaaaac taacttttcg atgcaacatg
    10981 tttttacgaa cgaaggtccc ttcaccttgt cttcgaatca agtacccttc agccactaaa
    11041 tcatttaatg ctttaacgac tgttgtatta ctaacctgat agattttttt caaatcacct
    11101 tcggaataaa ttttagatcc gggtttaaat ttgccttcgt caatctcttt taaaatgttt
    11161 tgttttataa tttcatactt tggtagcaca cacaaacact tccgtccttt tattttaaag
    11221 ccatcataac aaaataattt cctcaagtaa acagaataaa caccccttct atgggaaggg
    11281 gcgtttagaa ttaaggttgt ttaccgatgt acgctaaaat accgccgtcc acatagagga
    11341 tatgaccatt tacaaagtca cttgcaccac ttgcaaggaa gatggccgga cctttcaagt
    11401 cttctgcttc tccccatctt tctgctggtg tttttgcgat aataaattta tcaaatggat
    11461 gtctttctcc atccggttga atttcacgaa gaggagctgt ttgcggtgtc gcaatataac
    11521 caggtccaat tccattacat tgaatattgt agcatccgta ttctgatgca atattacgtg
    11581 ttaacatctt caatccgcct ttagccgctg cataagccga aacggtttcg cgtcctaatt
    11641 cactcatcat tgaacaaatg ttaatgattt taccgccacc ctttttaatc attgaaggaa
    11701 taacggcttt tgatacgata aatggcgcat ttaagtccac atcgatcact tggcggaaat
    11761 cttcagccgc catttcatgc attggaattc gtttgatgat tccagcattg ttgactaaga
    11821 tatcaatcac gcctacttct gcttcgattt ttttcaccat ttcttgaaca ccaggttcat
    11881 ccgtaacgtc acaaacatat ccgtaagctt taataccctt ttccgcatag tttgctagcc
    11941 ctttttctac tccactttca tttctatcat taaatacgat agtggcacca gcacttgcta
    12001 aagcttctgc aatcgcaaac ccgattccat atactgcacc tgttacgagt gcaactttac
    12061 cttttaatga aaacatgtca tttgtcattt tacttactcc tcactattta agatcttgca
    12121 ttggaaccat atccatgtcg gtataggtga tgttttctcc agccattccc cagatgaatg
    12181 tgtagtttga ggttcccaca cctgagtgaa tagaccaact tggtgaaata catgcttctt
    12241 cgtttccaac cactaagtgt tttgtttctt gcggagttcc catgaagtgg aagactcttg
    12301 tatcaggttc catatcaaag tagaggtatg tttccatacg acgttcatgc gtatgacatg
    12361 gcattgtatt ccaagagcta cctggttcaa gcactgtgta ccctaattgt aattggcagc
    12421 tttcgcattg atttggatga acatattgat aaatctttct ttggtttaat gtattagatt
    12481 cacccatgca aagtggaaca attttttcaa tacttaattt tttatttgga tatttatgat
    12541 gtgctggaac ggacacaatg tagaatttcg cagggtcttc agcagaaaca cttgagaaag
    12601 caatttcttt tgtttctttc ccgatgtagt atccatcttg tttcaccata ttttctgaaa
    12661 caccatcaat ttcaatgaca cctgggcccc caatattaat cacacccatt tcacgtcttt
    12721 gtaggaagta gtccacgcct aactctgttg ataattcaat agttaatttc ccttcaactg
    12781 gcattacccc accaaaaatc atgcggtcgt tatgagtgta cgtcaacttc acttgatttg
    12841 gttcgaatac tttctcaact aagaattcct ttcttaattg ctccgtagag taatgtctga
    12901 tgtcctctgg actgtgtgta tatctcgttt ccatagttct cctccttaat tggttcataa
    12961 aagcttttta ctaccaaatc ttttttctct tttggtagcg ctctcgcgtt cagtctaaca
    13021 ctaaacgaaa ccagtttcaa gtaaaactta acgtgttaac acgtaaactt atgcttttca
    13081 aaagtttttc cggagtattt cacaatatcg ccgttgaatg catacttcta aaacgcatta
    13141 aaatagatat aaattagaat tagattccag ttcaaaattc tcgatttcac aaatcgaggt
    13201 gacaaaaatt ttttttgata tttttaaaaa aggtgcaata agaaaaatct tattgcacct
    13261 aaaatgaata gatggcgacc agtttttggc aattctagtt ttcgttttta ttcattttct
    13321 taataagtaa ttaaaatata ttgcatgatt attttcatgc ctttattctc ctaaaacgat
    13381 gactctatac tttcttgttc tttttcgtgt tttatcaaag tcggcaaaat aaacataccc
    13441 agttttacca attcctaatt gaccatccac tactgctaat gtttcacttg caccgataat
    13501 ggtggccttg atatgcgcat ctccattcca aaggtagcta cgatttccat ttggtagcca
    13561 ttcttctgga ttaggccatg attccacttc tttatagtgt gcttcgcctg gatacttgta
    13621 actttgcgca tttatatggg gcggaactat actttctaag attcgattca aatctaattg
    13681 taagaaatcc actccgtctt catcacggtc atgtgtatct tcttcaaaga aaatagaaca
    13741 tgtcgtatgt gctgttacaa ctgtacaagt tcccgattga ataccacttt ctaaaatagt
    13801 atcttttaca ctttgcgtaa tatccacata ggatacagcc ttttcttttg tttcaacttt
    13861 aaactctttt tgaaatacac tcatgattaa ccctcattca tcacttgatc aatcgcttca
    13921 accattaatc tcaacatttc ttttggattt tcagctttta cgataccact tgtgcaacca
    13981 gtaccttctg cgcctaattt aatagtccga tagacatcct tttcattcat aattcctgca
    14041 ccttgcatca taagaatatt aggatatact gacttcactt gttcattcgt tttcacaata
    14101 taagaatcat cgctggtttg tccagtacca ataagctctg tcggttcaca aagcataatt
    14161 gttggctcta acattgcaag cattctcgct tcttgaatag aatccgcaca tagaactgta
    14221 atcattccta gttcttttgc tttatttacg gacgccacta cttgtgatgt tgttaatgga
    14281 tgttcggcgt ggtttaagaa agtggctctt gcaccagcat tatataacga tgctggtaat
    14341 acagctccca ttcccctacc gcaatcaatt ccatctaaat gttgagccgt tacaataata
    14401 ttttttgttt cactcgcaac tctactaata tctgcatatg ggcaagtcac aaaaatacta
    14461 atttcaggat aaacctctgc taaatggtcc gcttctttag ctaactctag taattcatca
    14521 ccaaataaat atgatttggg attgaagata aagaatttgc tttttacaat tttcatattt
    14581 ctcctcctaa tttatttggt ttcaaatact ttgtaataat gttcaagagt attttccata
    14641 ccctttcttg ataaattcac aatatcaata tagttttcta catatccatc agcaactgct
    14701 ttggcaccag agtatataaa atcactataa atgttaatct ttgaaatacc atgtgtagca
    14761 caacgattga ggttatcatc tccagaacct gacccgccat gcaataccaa aggaatagta
    14821 agtgcttcat tcaactcttc taaccgttca aagtttaatt ttggagtacc tttatacaac
    14881 ccatgagaag taccaataga aacagctaat gaatctacat tcgtttcttc aacaaatttc
    14941 ttagcatctt caacactcgt ataaatggat ttcacctctt cattattcga agcagcattt
    15001 gctccaacaa atccaatttc agcttctaca tctacaccaa attgatgtgc gtattcgaca
    15061 acttctttcg atttacgaac attttcctca aatgtatcta atgaagcatc aatcatgact
    15121 gaattaaatc caagttcaat agcacgttta atgaattcaa aatcttgacc atgatcgaga
    15181 tgcaacacaa ccggagtatt tgttacttct tggaaataac gtccaattgc agctgcgtct
    15241 tctaaactta aatattgaga atgtacttgt gcaaatgcta aaattaatgg taattctaat
    15301 ttttcactag tctcagaata tgctttagca caattccaat caatcatatt agctgctgga
    15361 actgcataat gttccttttt cgctttaccc agtacatctc tcattcttgc tttcatgatt
    15421 aagcctccaa tgcttgtttc aattcattcc atagagaatc aattcccatg cctgtaagta
    15481 atggcaatcc cataatcact ttatctttta cagaatcagg aactactgtt gtagaaataa
    15541 ctacgtcata ttcatcaagt ctatttactt cttctgatac tttagattgg aatacttgca
    15601 ctttatcttc gtacccatta tccttcaacc actgttttac tttaccaaca acaacagtag
    15661 atgtcgcaat accagaacca cacataacta ataatttttt cataatattc actcctattt
    15721 tgcaactcga actttattaa catagaatag accagctaat gatccaacta atacaactgt
    15781 taaaataatc catggggctg tttgcccagc aaagacaaat aaccaagttg gccataaccc
    15841 accttctgct agcgctgtaa tacttgtatt tccacctaaa tcaaagttag ctgcttttgc
    15901 tgcttgcgta acaagaggcg caacccatga tgtaatatag aaaatacttg tcatataaat
    15961 cgctccaccg acaactgtac gaacaatatt tcctttaaat acagctgcca ttaagcagac
    16021 tacgaatggg attgttgcaa ggtcaccaaa tggtaatgtt tggtttccag gcaaaatgac
    16081 tgcaagtaaa attgtaattg gtactaaaag taatgaactt gataatactg ctggatgtcc
    16141 tactgacaat gctgagtcca taccaatatt tacttcacga cctgggaaac gtttttgaac
    16201 gaattcgtta gcagcttctg agattggcgt taaaccttcc attaatagag acaccatacg
    16261 tggcataaga accattacag cagacatttt aacacctaat tgaagaactc cttttgcatc
    16321 ataaccagct aaaagaccca ttgcaatacc aattaataag cctaaaactg tactttcacc
    16381 aaacacacca aatttctttt ggatactttc tgggttagct tcaattttgt taaatccagg
    16441 aatacggtca aataaccagt tcaaaggaat cgcaactagg aatccaggtg ctgaagttcc
    16501 gtgtgggaat gtaatattag gaaatccata gaattttttg attgctggtg caataagatc
    16561 tgctaataag taaatcatta ctaaataagt agcagttgca tataaaccca atgtgaaatc
    16621 ttgtgtaact gcgtaaacta gtgaagcaac aaaggctgca tgccagaaat tccacatatc
    16681 aaccatcaat gtttttgtta accctaagaa taaaagtaaa atgttaatac cgataccaat
    16741 tggaatagca agcgaaccta acaatgtacc ataggaaatt gctgcagcgg ctggccaacc
    16801 aacgtcgatt gttgttaagt tcaagccaaa acgttcaacc attgcttggg cagcaggtcc
    16861 taagctacct cctagcaaat cgactaccat atttaatcct acaaaaccaa ccccaacaat
    16921 aatacctgat acaaaggctt ttttaggttt tgttccaaga gccataccaa aaataaaaat
    16981 taaaattggt agaacaacaa tggctcctaa atcaataaat ttttgaaata attctaacat
    17041 tataaattcc tcctaatgta taaaataatc agaatatcag tctgatactg atgatttagt
    17101 aactttctgt cctcctctcc tattactaaa ccatttaatt atttaatatt ttttaatgct
    17161 aatacttctt taatctctga gttaggtgta ttctcatcca tgctcaagat tctttccatt
    17221 atgtctttat cctgtaagac ttccatcagt tgttgaagca tcgtcaattg actgtgtgct
    17281 tctttcagcg ctagcataaa aattactctc actggaactt caacatcatt tgttcccatc
    17341 tgataaaaag aaacagcatc tttcaatgtc attaacgcta tttgctcttt attcacatgt
    17401 tctatatctg tatgtgggat agcaattcca tacgcttcaa attgcaatcc tgttggaaaa
    17461 acatgctctc tatcaataat tgcttgttta taagattctt ttacaattcc tttactgatt
    17521 aatttatcag ccaaatagct taacgcttct tctttcgttt tcatgtttgg aacattcatt
    17581 tcaaagactt cctcatttag cat
//
