LOCUS       NZ_MWQX02000053         5835 bp    DNA     linear   CON 01-FEB-2024
DEFINITION  Klebsiella pneumoniae strain MEC097 scaffold_52, whole genome
            shotgun sequence.
ACCESSION   NZ_MWQX02000053 REGION: 2616..8450
VERSION     NZ_MWQX02000053.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06458378
            Assembly: GCF_004368375.2
KEYWORDS    WGS; RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group;
            Klebsiella; Klebsiella pneumoniae complex.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Arya,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2020) Engineering, University of Georgia, 220
            Riverbend Rd, Athens, GA 30602, USA
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Arya,A.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) Microbiology, University of Georgia, 120
            Cedar St. Dept. of Microbiology, Athens, GA 30602, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            MWQX02000053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: A5-miseq pipeline v. 20160825
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 256x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/01/2024 04:38:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,675
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 137
            rRNAs                             :: 9, 16, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 16, 14 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 88 of 253
            Pseudo Genes (incomplete)         :: 172 of 253
            Pseudo Genes (internal stop)      :: 30 of 253
            Pseudo Genes (multiple problems)  :: 29 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="scaffold_52"
                     /strain="MEC097"
                     /isolation_source="raw waste water"
                     /db_xref="taxon:573"
                     /geo_loc_name="USA: Middle Oconee Water Reclamation,
                     Athens, GA"
                     /collection_date="2010"
     gene            1..816
                     /locus_tag="B4U61_RS26520"
                     /old_locus_tag="B4U61_026100"
     CDS             1..816
                     /locus_tag="B4U61_RS26520"
                     /old_locus_tag="B4U61_026100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="WP_117259872.1"
                     /translation="MKLGYETNTWGGVVGHPGGVTSIKDAYYLANGSSEQAIRDIAAV
                     GYNGFEMFDGNLMQFRDDKQAFRDLLQLTSQTFIGVYTGANFIYPDILEEELLKFRNV
                     AELTAELGGEHFVIGGGAIRGTGIRPDDHKLLADGLNQAMQIAEQAGLVASYHPHLGS
                     LVESPEQLDQIMTLTDIHLCPDTAHIEAGGGDPADVVRRYGKRIKYIHFKDLKNNEFL
                     PLGSGVQNFDAIVSALKEHDYDGWITVELDSYADPRAGAQISKDFLKKHFDFV"
     gene            944..2095
                     /locus_tag="B4U61_RS26525"
                     /old_locus_tag="B4U61_026105"
     CDS             944..2095
                     /locus_tag="B4U61_RS26525"
                     /old_locus_tag="B4U61_026105"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="WP_048268701.1"
                     /translation="MHNNNRSTLSSTVRSLLIAGGLITASLAFSTGVSAADLTINQSL
                     PDSAILSKGPGGESAVSAKTLSLSETELQQIRDGGYTAAIAMHYAGNDWSQAQINGLK
                     AAFDRMGIDVVAVTDAQFKSEKQAADIETLLARHPDVIVSIPVDAVSTAHAFKRAAEA
                     GVKLVFMDNKPNGLEAPADYISVVSADNYGNGIEAAHILAKSLNGKGKIGVIYHAADF
                     FVTKQRTDAFEATIKSEYPGIEIVARGGISGPNDGEKVASAMLTKNPTLDGIFVVWDV
                     PAEGALSAARAAGRSDLAITTIDLGINVALDIARGGPIKGLGAQLPYEQGTAEAILAG
                     YALLGKKAPSYVAVPALSVTQDNVLDAWQLVYNQPAPSMIQDVAKRNRK"
     gene            2123..3292
                     /locus_tag="B4U61_RS26530"
                     /old_locus_tag="B4U61_026110"
     CDS             2123..3292
                     /locus_tag="B4U61_RS26530"
                     /old_locus_tag="B4U61_026110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]; GO:0016651 - oxidoreductase activity,
                     acting on NAD(P)H [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="WP_048268702.1"
                     /translation="MKTLNVGMIGAGFMGKAHAMAYAAMPMFFWPAPAITVRKMVADV
                     TEEAAKDAALRLGFESYTADWRDIINNPEIDIVDIVTPNDSHAEIAIAAARAGKHIIC
                     EKPLARGAEESKTMLDAVQAAGVKHMVAFNYRRTPAVALAKKYIDEGAIGKVLNFRGT
                     YLQDWSADPNSPLSWRFQKSIAGSGTLGDIGTHVLDFARYLVGDIAEVMATTKTWINE
                     RPVHAGGVDKLGTVKSSGDVEKRPVDVDDEVLSMLRFENGAIGSIEATRNGYGRNNFL
                     TFEVHGEKGSISFNYERRDELKVFFKDDPEDRRGFRTVYTGPAHPYGDGLWPIPALGI
                     GYGETKIIETYDLVKSIVEDTDVSPNFADGYRIEQIADAILKSAQEGRWVSVDEI"
     gene            3372..4871
                     /locus_tag="B4U61_RS26535"
                     /old_locus_tag="B4U61_026115"
     CDS             3372..4871
                     /locus_tag="B4U61_RS26535"
                     /old_locus_tag="B4U61_026115"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="WP_048268703.1"
                     /translation="MDTQVHLKMSNICKTFNGIPVLEKVDFELRKGEVHALMGGNGAG
                     KSTLMKILTGVYRADEGAVLLDGQPIEIQNTEDAEAHGIAMIFQEFSLVPSLTVAQNI
                     FLNREPRTAMGLIDDRACVRKTRELLQELNVDIDPNTPVEQLGVGYWQMTEITKALSK
                     NARILIMDEPTSSLTENETEVLFRFIKKLKEKGISVIYISHRMDEIFEICDRTTILRD
                     GKWVLTESHDSLTIERMIEHIIGGNMEQAFVWSERQYDRTGEPLLRVSDLHSGSRVRG
                     VSFDLHRGEILGIAGLMGSGRSEAMRALFGIDPIDRGEVFINGSKTTIRRPGDAIAAG
                     LALVPEDRRVQGLVLDQDVKSNMLLPILNRIKKGLFVDDPAGEAIVQDYVRKLNIKTD
                     NIFKAANLLSGGNQQKIVLAKWLVNAPDILLLDEPTIGVDIGAKTEIMEIIRQFADGG
                     KAVLVVSSELTELLALADRVIVFHQGSVAQQFDRKNIASEEVLQHAIQG"
     gene            4855..5835
                     /locus_tag="B4U61_RS26540"
                     /old_locus_tag="B4U61_026120"
     CDS             4855..5835
                     /locus_tag="B4U61_RS26540"
                     /old_locus_tag="B4U61_026120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292825.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_048268704.1"
                     /translation="MQSKANTLPASTRFSQRGFDWRRYIVYVAFVAVFFYFSVTLYDE
                     GFLSTNNLLNIARQTAMIAIMAVAMTFVIGAAQIDLSVGSVAALSALISALAMQAGLG
                     MFGGIIAGLGTGALIGLINGLLITQIAIPAFLVTLGMMIITKGLAMWVTDTAPVPILN
                     ESFTFLFGSGDLGPIPVLLVWMVVIGIIGHVLLRKTAYGRRTLAVGGNEKAAEFSGVH
                     VKRIKLLVMVGSGMLAGLAGMLYAGRMYSARFTFGEGDELSVIAAVILGGTSLFGGTA
                     TVIGTIFGALMIGMINNGLIIMGLDVSQQMVVKGLIIILAVAFGKKAAQR"
ORIGIN      
        1 atgaaactag gttatgaaac aaatacttgg ggtggagttg tcggacatcc gggcggtgtg
       61 acctcaatca aggatgccta ctatttggcc aatggttcca gcgaacaggc cattcgcgat
      121 atagcagcgg ttggctataa cggcttcgag atgttcgatg gcaacctgat gcagtttcga
      181 gatgacaagc aggcgttccg tgacttgctg cagctaacat cgcagacctt catcggtgta
      241 tataccgggg ccaatttcat ttatccagat attctcgaag aagagctgct gaagttccgc
      301 aatgttgccg aattaactgc cgaactggga ggtgagcatt ttgtcatcgg tggaggagca
      361 attcgaggta ccggtatccg acccgatgat cacaaattgc tggccgacgg cctgaatcag
      421 gctatgcaga ttgcagagca agcgggactt gtcgccagtt accaccccca tttgggcagt
      481 ctggtcgaat caccggagca acttgatcag attatgactc tgaccgatat ccatctgtgc
      541 ccggataccg cccatatcga ggcgggcggt ggagaccccg ccgacgtagt acgtcgttat
      601 ggtaagcgta ttaagtatat acatttcaaa gacttgaaaa ataatgagtt tctgccgctg
      661 ggtagcggcg tgcagaactt cgatgccatt gtcagtgcgc tgaaggagca cgattacgat
      721 ggctggatca ctgtagagct cgacagttat gcggatccca gggcgggtgc gcagatcagc
      781 aaagatttcc ttaagaagca cttcgacttc gtgtaatgca aatcgacagt caataaacac
      841 tttctccgcc ctggagatta tcgctgcaac aacaacagga gattatcgct gcaataacaa
      901 caggaactga tccatgcata acaataacaa caggagctta cccatgcata acaataacag
      961 aagtaccctc tcctcaactg tgcgctcgct gctgatcgcc ggtggactga taactgcaag
     1021 cctggccttc tcgactggcg tcagcgctgc agatctgacc atcaaccagt ctctgccgga
     1081 tagcgctatt ctcagcaagg gacccggcgg tgagagcgca gtgtcagcca agaccctgtc
     1141 gctaagcgaa accgaactgc aacagattcg ggatggcggt tatacggctg ccatcgcgat
     1201 gcactatgcc ggcaacgact ggtcccaggc acagattaac gggctgaaag ccgccttcga
     1261 tagaatgggt atcgacgtgg ttgccgtgac tgatgcccag ttcaagtcgg aaaagcaggc
     1321 ggccgatatc gaaacgctgc tggctcgcca tcctgacgtg atcgtcagta tcccggtcga
     1381 tgcagtctcg actgcccatg ccttcaagcg ggccgcggag gccggcgtca agctggtgtt
     1441 catggataac aagccgaatg ggctggaggc accggctgat tacatcagcg tggtgtccgc
     1501 cgacaactac ggcaatggca tcgaagccgc gcatatcctg gccaaatcac tgaatggcaa
     1561 gggcaaaatc ggcgtgatct atcacgcggc tgactttttc gtgactaagc agcgcaccga
     1621 tgccttcgaa gccactatca agagcgagta ccctggaatc gagatcgtcg cccgtggcgg
     1681 tatttccggc ccgaacgatg gtgaaaaagt cgcctcagcc atgctgacca agaatccgac
     1741 gctcgacggc atcttcgtag tctgggatgt gccggcagag ggagcactgt ccgcggcccg
     1801 tgcggctggc cgcagtgatc tagcgattac caccatagat ctgggtatca acgtagcgct
     1861 cgatatcgca cgcggcggtc cgattaaagg gctcggcgca cagctgcctt atgagcaggg
     1921 tactgctgaa gcgatcctgg ccggctacgc actgctgggt aagaaagcac cgagctatgt
     1981 cgccgtgccg gcgctgagcg tcacccagga caacgtactg gatgcctggc aactggtcta
     2041 taaccagccg gctccctcaa tgattcaaga tgtcgccaag cgaaaccgaa agtaatgccc
     2101 taacgagagg attaacagaa taatgaaaac actgaatgta ggtatgatcg gcgccggttt
     2161 tatgggaaaa gcccatgcga tggcgtatgc agcgatgccg atgtttttct ggccggcgcc
     2221 agcaatcacg gtacgtaaga tggtcgccga cgtgaccgaa gaggccgcta aggacgctgc
     2281 actgcgtctg ggcttcgaga gctatacggc tgactggcgt gacattatca acaacccgga
     2341 gatcgatatt gtcgatatcg tgacgccgaa tgactctcat gccgagattg cgattgccgc
     2401 ggcccgggct ggtaagcaca ttatctgcga gaagccgctg gcgcgtggtg ccgaagagtc
     2461 caagaccatg ctcgacgcgg ttcaggcggc aggtgtcaag cacatggtgg ccttcaacta
     2521 ccgccgcacc ccggccgtcg cgctggcgaa aaagtatatc gacgaaggtg ccatcggcaa
     2581 ggtgctgaat ttccgcggta cctatctgca ggactggtcg gctgatccga attcgccact
     2641 gtcctggcgc ttccagaagt ctattgcagg ttctggcacc ctgggcgata tcggcaccca
     2701 tgtgctcgat ttcgctcgct acctggtcgg tgatatcgcc gaagtcatgg cgactaccaa
     2761 gacctggatc aacgagcgtc cggtgcatgc cggcggtgtc gacaagctcg gcacggtcaa
     2821 gagcagtggc gatgttgaaa agcgcccggt cgatgtcgac gatgaggtgc tgtcgatgtt
     2881 gcgcttcgaa aacggtgcta tcggcagcat cgaggcaacc cgcaacggtt atggtcgcaa
     2941 taacttcctg accttcgagg tgcacggtga aaaagggtct atctctttta actacgagcg
     3001 tcgcgacgag ttgaaggtgt tcttcaagga tgatcctgaa gaccgacgtg gtttccgtac
     3061 cgtttatacc ggtccggcac atccgtacgg tgacggcctc tggccgattc ctgcactggg
     3121 catcggctat ggcgagacca agatcatcga aacctatgat ctggtcaagt cgatagtcga
     3181 agacacggac gtatcgccta actttgctga cgggtaccgt atcgagcaga ttgccgatgc
     3241 gattctgaag tcggcgcagg aaggtcgttg ggtttcggtt gacgaaatct gatactgctc
     3301 tcgctgatta ctgctttatc tgagccctcc gtcgcttgtg gcggagggcc acattcgcaa
     3361 aggtacgcac gatggatacc caagttcatt tgaaaatgtc caacatctgc aagactttca
     3421 acggcatacc ggttctcgaa aaagtcgact tcgagctgag gaagggcgaa gtgcatgccc
     3481 tgatgggcgg caacggtgct ggcaagtcca cgcttatgaa gatcctgacc ggcgtgtatc
     3541 gggctgatga gggtgcggta ctgctggacg gacagccaat cgagatccag aacaccgagg
     3601 atgcggaagc ccatggcata gccatgatct ttcaggagtt cagcctggta ccgtcgctga
     3661 cggtcgcgca gaatattttt ctgaaccgcg aacccaggac tgctatgggg cttattgacg
     3721 accgggcctg tgtgcgcaag acccgggagt tgttgcagga actcaacgtt gatatcgatc
     3781 ccaatacgcc ggttgaacaa ctcggcgtgg gatactggca gatgacggaa attaccaagg
     3841 ctctttccaa gaacgccagg attctgatca tggatgagcc gacctcctcg ttaacggaaa
     3901 acgagaccga agtcctgttc aggtttatta aaaagctcaa ggaaaagggg atttccgtaa
     3961 tctatatctc gcaccgtatg gacgagatat tcgagatctg cgatcgcacc accattctac
     4021 gagacggtaa atgggtgctg acggaaagtc atgacagcct gaccattgag cggatgatag
     4081 agcacattat tggcggcaat atggagcagg cgttcgtctg gtccgagcgc cagtacgacc
     4141 gcacgggcga gccactgctc cgagtgagcg atctccattc cggtagccgt gttcgcggcg
     4201 tcagtttcga tcttcaccga ggtgaaattc tgggtattgc cgggctgatg ggcagcggcc
     4261 gctctgaagc aatgcgtgca cttttcggaa tcgatccgat cgaccggggg gaggttttta
     4321 tcaatggttc gaaaacgaca attcgccgcc cgggcgatgc gattgcggcg ggtcttgcgc
     4381 tggtgcctga agaccggcgg gtacagggtt tggtgctgga tcaggatgtc aaaagcaata
     4441 tgttgctgcc gatcctcaac cggataaaga agggcctgtt cgttgatgat cccgccggag
     4501 aggcaatcgt tcaggactat gttcgcaagc tgaacatcaa gaccgacaat attttcaagg
     4561 ccgccaatct gctgtcaggc ggtaatcaac agaagatcgt gctggctaaa tggctggtca
     4621 atgcgcctga catactgctt ctcgatgagc caaccatcgg cgtcgatatc ggtgccaaaa
     4681 ccgaaatcat ggaaatcatc cggcagttcg ccgatggggg caaggcggtg ctggtggtgt
     4741 cgtcggaact taccgagctg ctggccctgg ctgaccgtgt catcgttttc catcagggaa
     4801 gcgtggcgca acaatttgat cgtaaaaaca tcgcatccga ggaggtattg caacatgcaa
     4861 tccaaggcta atactctgcc ggccagcacg cgcttttcgc agcgtggttt cgactggcgt
     4921 cgttacatcg tttatgtcgc cttcgtggcg gtgttcttct atttctccgt gaccctttac
     4981 gacgaaggct ttctgtcgac taacaacctg ctgaatatcg cccggcagac tgcgatgatc
     5041 gcgattatgg ccgtggccat gacctttgta atcggggccg ctcagatcga cctttcggtt
     5101 ggctcggtcg ccgcattgtc tgcattgata tctgcgctgg cgatgcaggc agggctcggc
     5161 atgttcggtg ggataatcgc gggcctcggc actggcgctc tgattggtct gatcaatggt
     5221 ctgctgatta ctcagattgc gataccagcg tttctggtaa cgctgggcat gatgatcatc
     5281 actaagggcc tggcaatgtg ggtcactgat actgcaccgg tcccgattct gaatgagagc
     5341 tttaccttcc tgttcggttc gggcgatttg ggtcccattc cggtgttgct ggtctggatg
     5401 gtcgtgatcg gcatcatcgg ccatgtgctg ctgcgcaaaa ccgcgtacgg cagacgcacg
     5461 ctggcggtgg gcggaaatga gaaggccgcg gaattctccg gcgttcacgt caagcgtatc
     5521 aaactgctgg ttatggtggg ctcaggtatg ctcgcggggc ttgccggcat gctatacgcc
     5581 ggacgcatgt actcggcgcg ctttaccttc ggcgaagggg acgagctgtc ggttattgct
     5641 gctgtcatcc tcggtggcac cagcctgttt ggcggcactg caaccgtcat tggtaccatt
     5701 ttcggtgcgt tgatgatcgg catgatcaac aacggtttga tcatcatggg gctggatgtc
     5761 agccagcaga tggtggttaa gggtctgatc atcattctgg ccgtggcttt cggtaaaaaa
     5821 gccgcacaac gctga
//
