LOCUS       LC529899                7951 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  Bacillus smithii S-2701M DNA, levoglucosan degrading gene cluster.
ACCESSION   LC529899
VERSION     LC529899.1
KEYWORDS    .
SOURCE      Bacillus smithii
  ORGANISM  Bacillus smithii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1
  AUTHORS   Kuritani,Y., Umemura,S., Sato,K., Kitaoka,M., Fushinobu,S., Dora,H.
            and Yoshida,N.
  TITLE     Levoglucosan metabolic pathway in Bacillus smithii S-2701M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Yoshida,N. and Dora,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2020) Contact:Nobuyuki Yoshida Shizuoka
            University, Graduate School of Integrated Science and Technology;
            3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka 432-8561, Japan URL
            :https://wwp.shizuoka.ac.jp/yoshida-cb-shizuoka/
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Bacillus smithii"
                     /mol_type="genomic DNA"
                     /strain="S-2701M"
                     /db_xref="taxon:1479"
     gene            1..822
                     /gene="lgdB1"
     CDS             1..822
                     /gene="lgdB1"
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010973915.1"
                     /note="LOCUS_10;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminase"
                     /protein_id="BCB28820.1"
                     /translation="MKFGYQTNTWGGVVGHPAGVTSVKDLYYLANGSTEEALRDISNA
                     GFKGFEIFDGNLMQYKDRKEEFKALLNQYSLEFIGVYTGGNFIYPDILEEELLKIKSV
                     AALASELGARHLVVGGGAVRANGILESDYEALADALDRVAEMAEEYQLIPSYHPHLGT
                     IVEAPDQLDKLMPLTKIHLCPDTAHIEAGGGDPVEVIKKYADRIQYVHFKDYENGEFL
                     PLGKGHQKFDEMIRLLEESNYQDWITVELDSYPDPKEGAEISRRYLTSFEKTNQV"
     CDS             850..2010
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_012707199.1"
                     /note="LOCUS_20;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="BCB28821.1"
                     /translation="MNRKYALFMTVLLSVCLAMAGCKSFAVGSSVTESTKGLPNTGPL
                     TINNKIADKEIFSKGPNGEKAVSAKTLKLTEADIQKIKKGHYTAAIVMHYTGNDYVTA
                     QLNALKASFKRMGIKIIAVTDAQFKAEKQVSDIETVLAKSPDVIVSVPVDPVSTADAY
                     KKAARAGVKIVFMDGVAKGLKPGKDYVSVVSGDNYGNGAEAAKIMGEKLHGKGKIGVI
                     FHDADFFVTKQRQMAFEETIKKKFPKIKIVVRGGITSPNDGEKVASGMLTKYRDLDGI
                     FAIWDVPAEGALAAARTAGREDLVITTTDLGTNVALEIASNGIVKGLGAQLPYDQGIA
                     EAILAGYALLGKQAPTYVATPALGVTKENLFDSWKLVYRQDVPKIVKEAAKQ"
     gene            2059..3243
                     /gene="lgdA"
     CDS             2059..3243
                     /gene="lgdA"
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_015888061.1"
                     /note="LOCUS_30;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="levoglucosan dehydrogenase"
                     /protein_id="BCB28822.1"
                     /translation="MKKLNVGMIGGGFMGKAHSLAYAGMPMFFWPAPAIPHRHTIVDV
                     TDQLAKDAAQRLGFEHYSTDWKQVVEDPNIDIIDIVTPNDSHAEIAIAAAKAGKHIIS
                     EKPLARNAEEAKKMLDAVKEAGVKHMVAFNYRRTPAVALAKKYIEEGKIGNILNFRGT
                     YLQDWSADPNAPLSWRFQKERAGSGALGDIGTHVIDYARYLVGEISDVMAIAKTWIGE
                     RPIQTSGFDSLGNVKIGADAPKGIVDVDDEFATLIKFENGAIGSIEATRNAWGRNNFL
                     TFEIHGDKGSLYFNYERRDELQVCFSDDPSDSRGFRTVYTGPAHPYGEHLWPIPALGI
                     GYGETKMIETYDFVKSIVENTEVSPNFEDGYRVALISDAILESAEKGEWVKLVEKAAE
                     LK"
     gene            3314..4801
                     /gene="rbsA"
     CDS             3314..4801
                     /gene="rbsA"
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:B8E365"
                     /note="LOCUS_40;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="BCB28823.1"
                     /translation="MLLMKNISKSFNGIKVLDSVDFSVNKGEVHALMGGNGAGKSTLM
                     KILTGVYTLDQGEIYIEGEKVSIQSPDDARKNKISMIFQEFSLIPTLTVAQNIFLTRE
                     SKTSFGLLDDKECIKKTEQLLKELEVDLDPNEVVGNLGVGYWQMTEIAKALSHEAKIL
                     IMDEPTASLTKKETEVLFSFINKLKAKGYSIIYISHRMDEIFEICDRITILRDGKHVA
                     TEDCKEIDLATVIQHIVGSEVDKAFEWKERSYSMNATPVLEVKRLSSGTKVKDVSFKI
                     FPGEILGVAGLMGSGRSEMARSIFGIDPQDSGEILVNGKKRSIKSADDAIKAGIALIP
                     EDRRIQGLVLEHSVKENLILPILSKLNKGWFIDEKKANEIALSFVKKLNIKTDDIFKT
                     ANLLSGGNQQKIVLAKWLANDPTVLILDEPTIGVDIVAKTEIIDVIRHLADSGKAILV
                     ISSELSELLAVSDRVIVMHEGTIVKELNRKEIHSEEELQHAIQGY"
     CDS             4782..5180
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /note="LOCUS_50;
                     frameshifted, deletion at around 5177;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCB28824.1"
                     /translation="MQSKDINSVPNGTLVAKNNRLRQLKKLDWRNYIVYLAFIGVIIY
                     FSISLYDEGFVTADNFLNIVRQTAMISLMGLAMTFVISTGEIDLSVGSITALSSLTAA
                     LALQSGLGLIGGVVVGLATGLLVGLVNGLL"
     CDS             5246..5785
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /note="LOCUS_60;
                     frameshifted, deletion at around 5177;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCB28825.1"
                     /translation="MWITDTAPVPIVNSTFNFLFGSGNVGPIPILLIWTLVFTIIAHI
                     LLRKTSFGRQTLATGGNESAARFSGVNTLKIKLLVFLGSGLMAGLAGLLYAGRMHAGR
                     FTFGEGDELSVIAAVILGGTSLFGGVGTVIGTVIGSLMIGTINNGLIIMGLDVSEQMM
                     IKGAIIILAVAFGRKSMKK"
     gene            5808..6968
                     /gene="lgdC"
     CDS             5808..6968
                     /gene="lgdC"
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_013097689.1"
                     /note="LOCUS_70;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 3-dehydrogenase"
                     /protein_id="BCB28826.1"
                     /translation="MEKIKVGIIGTGFIGPAHIEAVRRLGFVEVVGVAGSSREKAEKI
                     AAELGIPKAYGDYREMIADPEIQVVHNCTPNYLHFTINKEIILAGKHVLSEKPLAMSS
                     KETEELVALAKKHKVVHGVNFNYRQFPIINQLASMIRNEELGKVHLIHGSYLQDWLLF
                     ESDYNWRLDPELSGKSRAVADIGSHWCDTVQFVTGKKIVEVFADLATVIPVRKKPSSH
                     VTTFGTQKGQEASEDVPIQTEDYASVLVRFEDGARGVFTVSQVSAGRKNRLSFEIDGS
                     QKSAYWNQEEPEKLWIGHRDRPNEILFADPALFTPEARSYIHHPGGHNEGWPDSLKNM
                     MLHFYTFIQEGKDPLRDKANFATFEDGHVSMCITDAILESHEQQKWVKVKAD"
     gene            6980..7951
                     /gene="lgdB2"
     CDS             6980..7951
                     /gene="lgdB2"
                     /inference="ab initio prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010989909.1"
                     /note="LOCUS_80;
                     involved in levoglucosan degradation"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoglucose dehydratase"
                     /protein_id="BCB28827.1"
                     /translation="MKLGVFTVLYQNLPFEEMLDKLCEMGVEAVELGTGNYPGNSHCD
                     PDELLNNPEKIKSFLHAIESRGLTISGLSCHGNPLHPNKKAAKEAHEVWRKTVLLAER
                     LEVPVINGFAGCPGDHENAKFPNWVTCSWPPEYLEILDWQWNQVVIPYWKEESKFAES
                     HGIKKIAFEMHPGFVVYNPETLLKLREHVGPAIGANFDPSHLVWQGIHPVEAIKKLGK
                     ENAIFHFHAKDIYLDEANIRVNGVLDTKHYSDILNRSWTFRSVGYGLDEKAWKDMLSA
                     LSAVGYDYVVSIEHEDMLASIDEGLGKAISLLKGALFKEPVEEMWWA"
ORIGIN      
        1 atgaaattcg gttatcaaac gaatacatgg ggcggagttg tgggacaccc cgcgggagtt
       61 acttctgtaa aggatcttta ctatcttgct aatggttcaa cggaagaagc tttaagagat
      121 atttcaaatg caggatttaa ggggtttgaa atttttgacg gcaatctcat gcaatataaa
      181 gatagaaaag aagaatttaa ggcattgctc aatcagtatt ctttagaatt tattggagtt
      241 tacacaggtg gaaattttat ttatccagat attttagaag aggaattatt aaaaattaaa
      301 agcgtagcag ctcttgcatc ggaattaggt gctcgacatt tagtcgttgg cggcggtgcc
      361 gttcgtgcca acggaatttt agaaagcgat tacgaagcac tagccgatgc tcttgatcga
      421 gtggcagaga tggcagaaga atatcagctt attccaagtt atcatccgca tttaggaacc
      481 atcgtggaag caccggatca gttagataaa ctgatgccgc tgacgaagat tcatttatgc
      541 ccggatactg cccatataga agcgggtggc ggcgatccag tggaagtgat caaaaaatat
      601 gccgatcgaa tccaatatgt ccactttaaa gattatgaaa acggcgagtt tcttccttta
      661 ggaaaaggac atcaaaagtt cgatgaaatg attcgattgt tggaagaatc caattatcaa
      721 gattggatta cggtggaatt ggatagttat cccgatccaa aagaaggagc ggagattagc
      781 cgccgctatt taactagttt tgaaaaaacg aatcaagttt gagtcaagaa gctttagagg
      841 ggggcattta tgaatcgaaa atatgcattg tttatgacag tccttttaag cgtctgtctt
      901 gcaatggccg gctgcaaatc ttttgcggtc ggaagcagcg ttacagaaag cacaaaagga
      961 cttccgaata ccgggccgtt gacgattaac aacaaaattg ccgacaaaga gattttcagc
     1021 aaaggcccga acggggagaa agctgtatcg gccaaaacgc taaaactgac ggaagcggat
     1081 attcaaaaga ttaaaaaagg gcactataca gcggcaatcg taatgcatta tacgggaaac
     1141 gactatgtaa cggcacaatt gaatgcgtta aaagccagtt ttaaaagaat gggaatcaaa
     1201 atcattgctg taacggatgc acaatttaag gctgaaaaac aagtatcaga cattgaaacg
     1261 gtattagcaa aatcacccga tgtgatcgtc agtgttccgg tggatcccgt ttcgacagca
     1321 gatgcttata aaaaagctgc tcgtgcggga gtcaaaatcg tgttcatgga tggtgttgcc
     1381 aaaggattga agcctggaaa agattatgta agcgttgtat cgggcgacaa ctatggaaat
     1441 ggcgccgaag cagcaaaaat tatgggagaa aaacttcatg gaaaagggaa aatcggcgtt
     1501 atttttcacg atgcagactt ttttgtaacg aaacaacgtc aaatggcttt tgaagaaacg
     1561 attaagaaaa agttcccgaa aatcaaaatt gttgtgcgcg gcggcatcac aagccctaac
     1621 gatggggaaa aagtagcttc cggcatgttg acgaaatatc gtgatttaga tggcatattt
     1681 gccatttggg atgtccctgc tgaaggcgct ttagcagcgg cgcgaacagc aggaagagaa
     1741 gatctggtca ttacgacgac agacttagga accaatgtcg ctttagaaat tgcttcaaac
     1801 ggcatcgtaa aaggactcgg tgcgcaattg ccgtatgatc aagggattgc ggaggctatt
     1861 ttggctggat atgcactgct tggaaagcag gctcctacct atgttgccac acctgcttta
     1921 ggagtgacaa aagagaatct ttttgattcg tggaaactag tttatcgcca agatgtacca
     1981 aaaattgtga aagaagcagc gaaacaataa cactataaca aaaaatagat gatgaattta
     2041 ataggaggta atgtcaaaat gaaaaaattg aatgtaggta tgattggcgg aggatttatg
     2101 ggaaaagcac actcacttgc ttatgcggga atgccgatgt tcttttggcc ggcacctgcc
     2161 attcctcatc gccataccat cgtggacgtg actgatcaat tggcaaaaga tgcagcgcaa
     2221 cgattaggtt ttgaacacta ctcaaccgat tggaaacagg tagtagaaga tccgaatatt
     2281 gatattattg atattgtcac tccgaatgat tctcatgcgg aaatcgccat tgcggctgca
     2341 aaagcaggga aacatatcat ttcggaaaaa ccgttagcca gaaacgcaga agaagcaaag
     2401 aaaatgttgg atgccgtcaa agaggccggc gtcaagcata tggttgcctt taactatcgc
     2461 agaacaccag ctgttgcttt agcgaaaaaa tatattgaag aaggaaaaat cggaaatatc
     2521 ctgaacttcc gtggaactta tttgcaagat tggtcagctg atccgaatgc accgttatca
     2581 tggcgattcc aaaaagaacg cgcaggatca ggagcattgg gagacattgg tacccatgtc
     2641 atagattatg cgagatattt agtcggtgaa atttcagacg tcatggccat tgcaaaaact
     2701 tggatcgggg aaagaccaat ccaaaccagc ggtttcgatt cattaggaaa tgtcaaaata
     2761 ggtgcagatg cgccgaaagg aattgtagac gtagatgatg aatttgcgac attaatcaag
     2821 tttgaaaacg gtgcgatagg aagtatagaa gccactcgaa atgcttgggg gcgcaataac
     2881 ttcttaacat ttgaaattca tggtgataaa ggatctttat attttaatta cgaacgccgt
     2941 gatgaacttc aagtatgttt ctctgacgat ccaagcgaca gccgtggatt caggacagtc
     3001 tataccggac ctgctcatcc ttacggagaa catttatggc ctattccggc attaggcatc
     3061 gggtatggag aaacaaaaat gatcgaaacg tatgatttcg ttaaatcgat tgtcgagaat
     3121 acagaagtat ccccgaactt tgaagatgga tatcgtgtag ctttgatttc cgatgccatt
     3181 ctcgaatcag ctgagaaagg cgaatgggtg aagctggtag aaaaagcggc ggaattaaaa
     3241 tagtcaactt tatagaggct gctcgtatag agcggcctct agacaaagaa ggtgatggag
     3301 tttggaacaa ccaatgctgt taatgaaaaa tatttccaaa tcatttaatg ggatcaaggt
     3361 gttggattcg gtagattttt ctgtcaacaa gggtgaagtg catgccctca tgggaggaaa
     3421 tggcgccggt aaatcgactt taatgaaaat cttgacggga gtatacacgc tggatcaagg
     3481 tgagatttac attgaagggg aaaaagtatc cattcaatcg cctgacgatg caagaaaaaa
     3541 taagatttcg atgatatttc aggaattcag tttaatcccc acattgacgg ttgcccagaa
     3601 tatttttctg acaagagaat cgaaaacttc tttcggactt ctggatgata aggaatgtat
     3661 caagaaaaca gagcaattat taaaagaatt agaagtagat cttgatccaa atgaagtggt
     3721 cggcaatctc ggtgtaggat attggcaaat gacagaaatc gcgaaagctt tatctcatga
     3781 agcgaaaata ttgattatgg atgaacctac agcttcttta acaaaaaaag aaacagaagt
     3841 tttgttttct tttatcaata aattgaaagc gaaaggctat tctattattt atatctcaca
     3901 tcggatggat gaaatatttg aaatttgcga tcggatcacg attttacgag atggaaaaca
     3961 tgtggcaacg gaagattgta aagaaatcga tttggctact gtcatccagc atatcgttgg
     4021 ttctgaagtc gataaagcgt ttgagtggaa agaaagatcc tactccatga acgcaacacc
     4081 ggtattggag gtaaaacggt tgtcttcagg gacgaaagtg aaagatgtca gctttaaaat
     4141 ctttcccggg gaaatcctag gtgttgccgg acttatggga agcggcaggt cggaaatggc
     4201 tcgatccatt tttggcattg atcctcaaga tagcggcgaa attctcgtaa atggcaaaaa
     4261 aagatccatt aagagtgccg atgatgctat caaagccgga attgccctta ttccggaaga
     4321 tcgaagaatt caaggattgg tgcttgagca tagtgtaaag gaaaatttga tcttgccgat
     4381 tttatccaag ctaaataaag gctggtttat tgatgaaaag aaagccaatg aaatcgcgct
     4441 tagttttgtc aaaaaactga atattaagac agatgatatt tttaaaacag ccaatttgct
     4501 gtctggaggg aatcagcaaa aaattgtatt agccaaatgg ctggcgaatg atcctactgt
     4561 tttaatattg gatgaaccta cgatcggtgt ggatattgtt gctaaaacgg aaattatcga
     4621 tgtcattcgc cacttggccg acagcggcaa ggcgattctc gtgatttcat ccgaactatc
     4681 tgaacttctt gcggtaagtg atcgagtgat cgtcatgcac gaagggacaa tagtgaagga
     4741 actgaataga aaggagattc attctgagga ggaacttcaa catgcaatcc aaggatatta
     4801 attctgttcc gaacgggaca ttagttgcaa aaaataatcg tttacgacag ttaaaaaaat
     4861 tagattggcg gaattacatt gtttatctgg cttttatcgg cgtcatcatc tatttttcga
     4921 tcagcttgta tgatgaagga tttgtcacag cggacaattt cctgaacatc gttcggcaaa
     4981 cggctatgat ttcccttatg ggattagcca tgacgtttgt gatcagcacc ggagaaatcg
     5041 acctgtctgt tggctcgatt acagcgctat cttcgttaac agccgctttg gctttacaat
     5101 cgggactggg attaatcggc ggagtcgtcg taggtttagc gaccggacta ctggtcggtc
     5161 ttgtgaatgg actgctgtaa cgaaagtagc catcccttct tttttggtga cgctcggaac
     5221 gatggggata gtaaaaggat tggccatgtg gattacagat accgcaccgg tgccgattgt
     5281 gaactccact tttaattttc tatttggttc gggtaatgta ggacccattc cgattttgtt
     5341 aatttggact ttagtgttta caatcatcgc tcatattctg cttagaaaaa cgtcctttgg
     5401 aagacagact ttagcaacgg gtggaaatga aagcgctgca aggttttcgg gtgtcaatac
     5461 gctaaaaatc aaattattag tctttttagg ttcgggtttg atggctggtt tagccggact
     5521 tctttatgca ggccgaatgc atgcaggaag gtttacattt ggcgaagggg atgaactgtc
     5581 ggttatagct gcggtcattc tgggcggaac cagtttattt ggcggcgtag gaaccgtaat
     5641 cggaactgtc attggatcac tgatgatcgg aaccattaat aacggattaa ttatcatggg
     5701 tcttgacgta agtgaacaaa tgatgatcaa aggtgccatt attattttag ccgttgcgtt
     5761 cggaagaaaa tcgatgaaaa aataaaacgt tggttgagga gtgcgttatg gaaaaaataa
     5821 aagtgggaat catcggaaca ggttttatag gacctgctca tattgaagcc gtaagaagac
     5881 ttggatttgt ggaagtcgtc ggagtggcag ggagcagtcg ggaaaaagcg gaaaaaatcg
     5941 cggctgaact gggaatcccg aaagcttatg gggattaccg tgaaatgatc gctgatcctg
     6001 aaattcaggt ggtgcataat tgtaccccga attatcttca ttttaccatt aataaagaga
     6061 ttattttagc aggcaaacat gttttatcgg aaaaaccttt agccatgtcc agcaaagaaa
     6121 cagaagaatt agtcgcgttg gctaaaaaac acaaagttgt tcatggtgtt aatttcaatt
     6181 acagacaatt tcctattatc aatcaattag cctccatgat ccgaaatgaa gaattaggaa
     6241 aggtccattt aattcacggc agttatttgc aagattggct gttgtttgaa agcgattata
     6301 attggcgctt ggaccctgaa ttaagcggaa aatcgagagc tgtagctgat atcggatccc
     6361 attggtgtga tacggtccaa ttcgtaaccg gcaaaaaaat tgtggaagta tttgccgatt
     6421 tggccacggt gataccggtt aggaaaaaac catcatccca tgtcacgacg tttggaacac
     6481 aaaaaggaca ggaagcatcc gaagatgttc cgatacaaac agaagattat gcttctgtcc
     6541 ttgtacgttt tgaagatgga gccagaggtg ttttcacggt ttcacaagta agcgccggaa
     6601 gaaaaaacag gctcagtttt gaaattgatg gcagccaaaa atcggcctat tggaatcaag
     6661 aagaacctga gaaattatgg attgggcatc gggacaggcc aaatgaaatt ctctttgcgg
     6721 atcctgcttt attcacgccg gaagcaagat catatattca tcatcctggg ggacataatg
     6781 aaggttggcc ggactcttta aaaaatatga tgctccattt ttataccttt attcaagaag
     6841 gcaaagaccc actgagagat aaagcaaatt ttgccacatt tgaagatggg catgtatcga
     6901 tgtgtattac agatgccatt ttagaaagtc atgagcaaca aaaatgggtg aaagtgaagg
     6961 cagattagga ggtacattca tgaaactagg cgtatttacc gttctttatc aaaatcttcc
     7021 gtttgaagag atgttagata agctgtgcga gatgggggta gaggcggttg agctgggaac
     7081 aggcaattat ccgggaaaca gccattgcga tccggacgag ctgctcaata accctgaaaa
     7141 aatcaaatct tttttacatg cgatcgaaag cagagggtta accatcagcg gattaagctg
     7201 tcatggaaat ccccttcatc cgaataaaaa ggctgcaaaa gaagctcatg aggtttggcg
     7261 gaaaacggtt ctgttggcag aacgtctaga agtgcccgtg atcaatggat ttgccggatg
     7321 tccgggtgat cacgaaaatg cgaagtttcc aaactgggtc acttgttcct ggccgccgga
     7381 atatttagaa attctagatt ggcaatggaa ccaagtcgtt attccttatt ggaaagaaga
     7441 atccaagttt gcggaatcac acggaattaa gaagattgcc tttgaaatgc atcctggttt
     7501 cgttgtttac aatccggaaa ctttactgaa attaagggaa catgtcggac ccgccatcgg
     7561 tgccaatttt gatccaagcc atctcgtttg gcaagggatt catcccgttg aggcgattaa
     7621 aaaattagga aaagaaaatg ccatttttca tttccatgca aaagatattt atttggatga
     7681 agcgaatatt cgtgtcaatg gcgtattaga cacgaaacat tacagcgata ttttgaatcg
     7741 ctcatggaca tttagatccg ttggatatgg tctggatgaa aaggcatgga aagatatgct
     7801 cagcgctctt agcgccgttg ggtatgatta tgtggtttcc attgaacacg aggatatgct
     7861 tgcttccata gatgaaggct taggcaaggc gatttcttta ttaaaaggag cgctcttcaa
     7921 agagccggta gaagaaatgt ggtgggctta a
//
