LOCUS       NZ_GL882612            12502 bp    DNA     linear   CON 24-MAR-2023
DEFINITION  Bacteroides fluxus YIT 12057 Scfld11, whole genome shotgun
            sequence.
ACCESSION   NZ_GL882612 REGION: 2624..15125
VERSION     NZ_GL882612.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00189096
            Assembly: GCF_000195635.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Bacteroides fluxus YIT 12057
  ORGANISM  Bacteroides fluxus YIT 12057
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B.,
            Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C.,
            Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C.,
            Mitreva,M., Hou,S., Chen,J., Wollam,A., Pepin,K.H., Johnson,M.,
            Bhonagiri,V., Zhang,X., Suruliraj,S., Warren,W., Chinwalla,A.,
            Mardis,E.R. and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2011) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            GL882612.1.
            Bacteroides fluxus YIT 12057 (No 16S rRNA gene available) is a
            member of the Bacteroides division of the domain bacteria and has
            been isolated from the gut.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            
            Bacteria and source DNA were provided by Masami Morotomi, PhD
            (Yakult Central Institute for Microbiological Research, 1796 Yaho,
            Kunitachi, 186-8650 Tokyo, Japan). Isolation of bacteria and DNA
            preparation were funded by the Yakult Central Institute for
            Microbiological Research.
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were
            blasted against NCBI's non-redundant (NR) database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.0. Gene names are generated at the contig level and may not
            necessarily reflect any known order or orientation between contigs.
            
            The National Human Genome Research Institute (NHGRI), National
            Institutes of Health (NIH) is funding the sequence characterization
            of the Bacteroides fluxus YIT12057 genome.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: High-Quality Draft
            Current Finishing Status :: High-Quality Draft
            Assembly Method          :: Velvet v. 0.7.57
            Genome Coverage          :: 150.7x
            Sequencing Technology    :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/24/2023 12:23:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,612
            CDSs (total)                      :: 3,549
            Genes (coding)                    :: 3,463
            CDSs (with protein)               :: 3,463
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 20 of 86
            Pseudo Genes (incomplete)         :: 67 of 86
            Pseudo Genes (internal stop)      :: 11 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Bacteroides fluxus YIT 12057"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Scfld11"
                     /strain="YIT 12057"
                     /isolation_source="biological product [ENVO:02000043]"
                     /host="Homo sapiens"
                     /type_material="type strain of Bacteroides fluxus"
                     /db_xref="HMP:9446"
                     /db_xref="taxon:763034"
     gene            complement(1..2253)
                     /gene="bglX"
                     /locus_tag="HMPREF9446_RS02735"
                     /old_locus_tag="HMPREF9446_00611"
     CDS             complement(1..2253)
                     /gene="bglX"
                     /locus_tag="HMPREF9446_RS02735"
                     /old_locus_tag="HMPREF9446_00611"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826828.1"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="WP_009123870.1"
                     /translation="MKAANIFSACLFLLPLASCTQVTDSGRDAVIEQKIEALLSKMTL
                     EEKIGQMNQISSYGNIEDMSGLIKKGEIGSVLNEVDPVRVNALQRVAVEESRLGIPLL
                     MARDVIHGFKTIFPIPLGQAASFDPEVAKDAARIAAIEASSVGIRWTFAPMIDVSRDP
                     RWGRIAESCGEDTYLSSVMGAAMVRGFQGDSLNNPTSIAACAKHFVGYGAAEGGRDYN
                     STFISERSLRNVYLPPFEAAAKAGAATFMTSFNDNDGVPSTGNKFILKDILRDEWGFD
                     GLVVTDWNSAREMIAHGFAADDKEAAALAVNAGVDMEMVSYTFFKCLPEQVKAGKVKE
                     EVVDEAVRNILRVKFRLGLFDNPYVDEKSPSVMYDESHLAAAKRAAEESAILLKNEGE
                     VLPLKEGVRTVAIVGPMADAPHDQLGTWIFDGEKSHTQTPLSAIKAMYGDRVQVIDEP
                     GLAYSRDKSMAGISKAVSAAQRADVVIAFVGEEAILSGEAHCLADLNLQGAQSELIAA
                     LARTGKPLVTVIMAGRQLTIGKEAEASDAVLYSFHPGTMGGPAIADLLFGKAVPSGKT
                     PVTFLQTVGQVPLYYAHNNSGRPASSHFKPLDQVPLEASQTSEGSSSSYMDAGVQPLY
                     PFGYGLSYTTFKYGKPQLSSKKLSSGDVLTVTFTLENTGKYEGTEVAQLYVQDKVASV
                     TRPVKELKRFARVALKPGEKKTVTFELPVSELAFWNIDRVKTVEPGEFALWVAPDSQA
                     GEEVSFQVVD"
     gene            complement(2256..3548)
                     /locus_tag="HMPREF9446_RS02740"
                     /old_locus_tag="HMPREF9446_00612"
     CDS             complement(2256..3548)
                     /locus_tag="HMPREF9446_RS02740"
                     /old_locus_tag="HMPREF9446_00612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039968351.1"
                     /translation="MKNLLIFACLFFCGLFSGCSSSPKSGLEIRKTGDGHKLYIDGRE
                     TYIKGVGGAYRLDIASESGANAFRTWGGNVEEVKKNLALASENRMYVMQGIGMTKDSA
                     RYYDDTYKDKMRAEVRLLAETFKNDTSLLAWGIGNEIELGNANVAAAWQFVEELAQLI
                     KSIDKRHLVSTVISYNPKALDSIAMYAPTLDYVGINVYGPMDKVREVVDQSAYKGAFM
                     ITEWGPTGWWETATTEWKAPIEQTSEEKRQVYEERYTQYISQNPRCLGSFVFLWGQKE
                     ERTPTWFSMFVEDKVDKFPLRGEKTPMVEAMQRVWTGREPTETAPVIEGMTINDKHAI
                     DNIRIEAGVPFVANVAVTDKEDDRLTYVWEILKEATVLGFGGSYEPRPERYGEVVTSD
                     VNSYGTTIHIPGEYRLYGYVLDNTGFVSTVNVPFQVIK"
     gene            complement(3688..4560)
                     /locus_tag="HMPREF9446_RS02745"
                     /old_locus_tag="HMPREF9446_00613"
     CDS             complement(3688..4560)
                     /locus_tag="HMPREF9446_RS02745"
                     /old_locus_tag="HMPREF9446_00613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="WP_009123872.1"
                     /translation="MKNIFKIGLFALVTAFAMTSCDPQDGDDHSLGVKPQESQLSFTA
                     TPGSNPNVVTLKNTSSLKGLVVTWDLGNGVTAKGEEVVASYPFANTYTIAMTAYNTGG
                     STTITQTITIANNDESQIEPKAIILAGGLTGSKTWVFDRAHDGHFGVGPGAGNPDYNG
                     TPSWWSCPAEGKAECALYENEFSFHLDGGYNMTWVNKGKIYTNGAGKDKLPGVATVPG
                     AGDFDVEYIPKEAYTFTVDGDKLKLSDDAFFGHFAGTSTYTIKTLNENELYLECSSAV
                     ESGNGWWYRFVPKK"
     gene            complement(4590..6122)
                     /locus_tag="HMPREF9446_RS02750"
                     /old_locus_tag="HMPREF9446_00614"
     CDS             complement(4590..6122)
                     /locus_tag="HMPREF9446_RS02750"
                     /old_locus_tag="HMPREF9446_00614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="WP_009123873.1"
                     /translation="MKKYKIFTIAALLSSALVTTSCGDDFLTANSTEKPMAGAPATEG
                     TILSSLGSAYQILLFDSYANNNYNSILLMSDLRSDDLYKGGGDAGDQAALYSLSQFNM
                     SAAESLGGLWSIYFTGLARCNNAIIACANAEGVQERKVKQYNAEAHFLRAYYVHLLWK
                     FFGNIPYFEEPLQDPYMTKQISADEIYNEIMADLEVACEEGVMEMVNNSGDNKGRACR
                     AAALMLKARVVMYQKDQSRYAEIANDMATIIKSGKFELMDDFDAMWLDENEFCKESIF
                     ESNQLPEGKTWSSGWQGYGTNLPAFISPNELNDPSGVFKGGWGFAPVRQSAYDMYEEG
                     DLRRDASINDWRGASYGHRFQDTGLFQRKYAAREGYNPPPGDTDLNYCNNLRIFRYAE
                     TLLNYVELVKMDGVAEQQGVDAQECFNLIRKRAFGIDKPLAATAENIKSERHKEFLGE
                     GMRFYDLVRWGDAAAVLTENDAAHNSVRTYDDNKKLIPIPLNEIEKTKGTEFELKQNP
                     GY"
     gene            complement(6144..9326)
                     /locus_tag="HMPREF9446_RS02755"
                     /old_locus_tag="HMPREF9446_00615"
     CDS             complement(6144..9326)
                     /locus_tag="HMPREF9446_RS02755"
                     /old_locus_tag="HMPREF9446_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="WP_009123874.1"
                     /translation="MKKLLSVLFLLSFTLASVYAQNIQVKGTVVSGSDNEPLPGVNVV
                     VKGNTGIGTITGLEGEFTLSVPSDAVLSITYIGFKAQDIAVKGQHNLKIVLMEDTETL
                     DEVVVVGYGVQKKSVVTAAISRVGADELNAAKPSRVEDALKGKVSGVQITQSSGQPGS
                     DSKVRIRGIGTVNNSDPLYIVDGMPVDGGINYLNPVDIESVEILKDAASAAIYGARAA
                     NGVVLVTTKGGKTGKTTINYDFSYGWQNPWKKKAVLDATEYMTIMNEMDVNDGNAPRY
                     TQEQIANAGKGTDWQDETFNYDAPIQQHQVSISGGSDKVQYFLSLGYFNQEGIVGGDY
                     GKSNYERWSLRSNSTYTVFEDNSRKFLNKLKVGVNIGYSRGTSTGIETNSEYGSILGS
                     AIAFSPLVPVYASKEEGEAILKQYPTAVTDKDGNVFSLPPTGYQELANPVAQLNSPSV
                     TKNNEDKFVGSFWAELDILPGLKFKSSYGADLAFWGADGYGFEYYMGSMKKNEKSWVQ
                     SEMNRGFKWQVENVLTYNKTFAEKHNLTVVLGQSAQKYSVRKLGGSDYNLLDTDPNKA
                     NINSAIGDRDDERVWGGTDGYNFTALASYFGRIDYNFAERYMLQLTMRRDGSSRFGPD
                     HKWATFPAVSVGWNVWNEPYLADIQPSWFDSFKVRFSWGKNGNENIGNFRYAALMDGG
                     QNYYFGGGYVVKDASKGGVMQYGSSPAALANPNIKWEESEQIDLGFDARFFDARLNLG
                     FDYFKKKTNGMLMDQPIPSYVGQSAPMSNLGKMENWGIELEAGWKHTIKDFSYRISAN
                     ASYLKNKLINLGNASGETNYENAGASGVGDYVHAKNGEVWPYFYGMVTDGLFQNQAEI
                     DAYVNDKGEIMQPDAQPGDVRFVDFNGDGKVNDDDRTKIGKGMPDWTFGFTLGADWKG
                     FDLNLFFQGTIGNDIYDFAQRGDIPAMNRPTWILDRWMGEGTSNSIPRMTSANPNKNW
                     RSSDLYVKDGSYLRLKTAQLGYTLPASLTKKFSVQRLRVYVSAENLITITGYDGFDPE
                     LASGGYTTLGVDKGIYPQSRTISLGANITF"
     gene            complement(9617..12502)
                     /locus_tag="HMPREF9446_RS02760"
                     /old_locus_tag="HMPREF9446_00616"
     CDS             complement(9617..12502)
                     /locus_tag="HMPREF9446_RS02760"
                     /old_locus_tag="HMPREF9446_00616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_039968353.1"
                     /translation="MKNHKLYWLFSWLFLFPFVELFAGWNSFIVNFDKSLYGKGAQTW
                     QIAPYDDKWVYFANKNGMVQFDGNVWSVFPLNNSSDVRSVLPSAAQKRIYVGGINEFG
                     YYKPDADGSLVYHCMSDTLDGSIRFLGNVWGIHEVDNILYFQGDDRVIKYLNGKYTAI
                     EMDAKIDCSNMVNGILYVGTDRGVWLLVGNTFFPLQGGDTLASKRIRGIIPYKKGVLV
                     VTAYNGLYYCDGRTVEPFMTGAEDFMRENEVFCVAKKDDKIALGTIHKGLVLIDCSTG
                     QLKYFNENNGLRNNTVLSVSFDVAGNLWAGLDSGIDYVCLSSPFTNLYSYPYSYGTGY
                     TAAVENGYLYLGTNRGLYYTPYPVKLNGNLPDINPMPQSSGQVWNLCRIGDELFCLHD
                     RGIFQIRGTSVKRVTDITGAWSCQLVTGHTDLMYVGVYNGLYLVAKKNGEWTVACKID
                     GMGDSCRLFEQENEKVIWVYNTDHVTRIELTDDLTKVLNIKEYDKDDGFPAGRDMYVS
                     KVEGRIYFATSSGIYKYNPHKEIMEPCPDMNNLLNGSSVYSRLLEYNDKLISLSPHEI
                     CSANLGTYKRGSNTSITPIQQSLVELVPDFEAIIPLSDSLMIIPNEEGFALFSIPAVK
                     TRQDHAHSLYIKNMYLSYPKDSLVYTANFLGQKSFPQISYSQNSVRFEYGLPSFMQGD
                     DIHFQYRLNKGNWSDFTAVSTKEYSNLSEGEYTFEVKAVFPDGTTSLDEIMFRILPPW
                     YRSVPAYICYIVLALFALWYIYRWDDVRVRRKKQQAVIEKDKELHELEKGYEEEKARQ
                     EKQIMQLEKERLEYDLQHKSQEMANLMINLVRKNEMLTEIKSEIYKVSTLLKGEGTRE
                     GKQQLMLINSRIDANIQSDEVLKRIEDQFDLIHNNFMKRLHAKHPDLSNNERMMCAYL
                     KMNLSTKEIAPLLNISVRGVETIRYRLRKKFGLEREDSLTDYLNNKL"
ORIGIN      
        1 tcaatctact acctgaaagg aaacttcttc tccagcctgg ctatccggtg ccacccagag
       61 agcaaactct cccggttcta ccgttttcac cctatctata ttccagaaag caagttcgct
      121 taccggaagt tcaaacgtca ccgtcttttt ctcgcccggc ttcaatgcaa cacgggcaaa
      181 gcgtttcaac tcttttaccg gacgggttac ggatgcaacc ttatcctgta cataaagttg
      241 ggccacttcc gtaccttcat atttgccggt attctctaaa gtaaaggtta ctgtcaacac
      301 gtctccggaa gaaagtttct tggaggaaag ctgtggtttg ccatacttga aagttgtata
      361 agacaagcca tatccaaacg gatagagagg ttgaactcct gcatccatat aagaggaaga
      421 acttccttcg gaggtctggc tggcctccaa tggcacttga tccagcggct tgaagtggga
      481 agatgccggc ctgcccgaat tattgtgtgc atagtacagg gggacttgtc ccaccgtctg
      541 caagaaagtg acaggagtct tgccgctcgg cacagctttt ccgaacaaca aatcggctat
      601 ggcaggacct cccatagttc ccggatggaa agagtacaac actgcatccg aagcttccgc
      661 ctctttaccg atagtaagct gacggcctgc cataatcaca gtaaccaggg gcttgcctgt
      721 tcgggccaat gcggcaatca gttcggattg cgcgccttgc aggttcaagt ctgccagaca
      781 atgtgcttca ccggacaaga ttgcctcttc tccgacaaac gcaattacca cgtccgcacg
      841 ttgcgccgcc gagactgcct ttgaaatacc tgccatactc ttgtcacgac tataagccag
      901 acccggttca tcaatcacct gtactctgtc tccatacata gccttgattg ccgacaacgg
      961 agtttgtgta tgcgacttct caccatcaaa aatccaagtg cccagctggt catgaggagc
     1021 atccgccatg gggcccacta tagctaccgt acgtacgccc tctttcaaag gcaacacttc
     1081 cccctcattc ttcaacaaga tggcggattc ttccgctgcc cgtttagcgg cggccaaatg
     1141 agattcatca tacatcaccg aagggctctt ctcatccaca tacggattgt caaacaatcc
     1201 cagacggaat ttgaccctca aaatgttccg caccgcttca tctaccacct cttctttcac
     1261 ctttccggct ttaacctgct cgggcaggca tttgaagaaa gtataactca ccatttccat
     1321 atccactccg gcattcaccg ccaaagcggc tgcttctttg tcgtcggcgg caaatccatg
     1381 ggctatcatc tccctggcag aattccagtc tgtcactacc agtccgtcga agccccattc
     1441 atcacgaagt atatccttca gaataaactt gtttccggta gaaggcactc catcattatc
     1501 attgaacgaa gtcatgaaag tagccgcacc tgctttggcc gccgcctcaa aaggaggcag
     1561 ataaacattg cgcaaactgc gttccgaaat aaaggtagag ttatagtcac gtcctccctc
     1621 tgctgctcca taaccgacaa agtgtttggc gcaggcggca atcgaagtag gattgttcaa
     1681 agaatcgcct tggaaacctc tcaccatagc cgctcccatc accgacgaaa gataggtatc
     1741 ttcgccgcaa ctctcggcaa tacgtcccca acggggatcg cgggagacgt caatcatcgg
     1801 agcaaaggtc caacggatac ctacggaaga ggcctcgatg gccgctatcc tggcagcatc
     1861 ttttgccact tccgggtcaa aagacgcagc ctgccccaaa ggaataggaa aaatcgtttt
     1921 aaaaccgtga atgacatcgc gtgccatcag caaaggaatg cccaaacggg actcttctac
     1981 cgccacacgt tgcagcgcat tgacacgcac cggatctact tcattcaaaa cagaaccgat
     2041 ttcacctttc tttatcagtc cactcatgtc ctcgatattc ccgtaagaag aaatctggtt
     2101 catctgcccg attttttctt ccaaggtcat cttggacaac aaagcctcaa tcttttgttc
     2161 aataactgca tctcttccgg aatccgtaac ttgcgtacat gacgccaaag gcaacaggaa
     2221 caaacaagct gagaaaatat tagctgcttt cattcttatt taattacttg aaatggaaca
     2281 ttaacggttg atacaaaacc tgtgttatcc agaacatatc catacaaccg gtattccccg
     2341 ggtatatgga tggtggttcc gtaagaattt acatctgaag tcactacctc accgtatctt
     2401 tccggacgag gctcgtatga accgccgaat cccagtacgg tagcctcttt gagaatctcc
     2461 cagacatagg tcaaccggtc atcttcttta tctgttacgg ccacattagc cacaaaagga
     2521 actcccgctt caatacggat attatcgata gcgtgtttgt cgttgatggt catcccctcg
     2581 attaccgggg ccgtttccgt cggttcccta cccgtccata ctctttgcat cgcttctacc
     2641 atcggcgtct tttctccacg cagcggaaac ttgtccacct tatcttccac gaacatactg
     2701 aaccaggtag gtgtgcgttc ttctttctgc ccccacaaga aaacaaaaga acccaaacaa
     2761 cggggatttt gcgaaatata ttgggtataa cgttcctcgt aaacctgccg tttttcctcg
     2821 ctggtttgtt caatgggagc tttccactct gtagtggcag tctcccacca gccggtcggt
     2881 ccccattccg taatcataaa ggctcctttg taggccgatt ggtcaactac ttccctcact
     2941 ttatccatcg gaccatacac attgattccc acatagtcca gcgtaggagc atacatggct
     3001 atggaatcca aagctttcgg attgtatgaa attacagtgg aaaccaggtg gcgtttatcg
     3061 atggatttta tcaattgtgc cagttcttcc acaaactgcc atgccgcagc cacattggca
     3121 ttacctaatt ctatttcatt accgataccc caagccaaca gactcgtatc gtttttgaat
     3181 gtttctgcca gcagacggac ttcagcacgc attttgtctt tatatgtatc atcgtaatac
     3241 cgggcagaat ctttagtcat gccgattccc tgcatcacat acatgcggtt ctctgaagcc
     3301 aatgccaaat tcttctttac ttcctccaca ttgcctcccc atgtcctaaa agcattggcg
     3361 ccactctcgg aggcaatatc cagacggtaa gcgccaccta cacctttaat ataggtttcc
     3421 cttccatcga tatagagttt atgtccgtct ccggttttcc gtatttcaag gccactcttc
     3481 ggtgaagaag aacaacccga aaacaaaccg cagaaaaaaa gacaagcaaa aatcaacaaa
     3541 ttcttcatca taaaaacatc tttaaaaata agagcgaata tcagtcatcc ctaaatattt
     3601 cttcatttta ctacaactat cagagaaaga aaaaggctgt gccggaagtg acacagcaca
     3661 gcctccttgc atttatccaa tcattcccta ttttttagga acaaagcgat accaccagcc
     3721 attaccagac tctacagcac ttgagcactc cagataaagt tcattttcat tcagagtctt
     3781 gattgtataa gtagatgttc ccgcaaaatg accgaagaaa gcatcgtcac tcaatttcaa
     3841 tttatcaccg tcaactgtaa aagtataggc ttctttagga atgtactcca catcaaagtc
     3901 tccggcaccg ggtacagtcg ccactcccgg aagtttatcc ttgccggcac cattcgtata
     3961 aattttccct ttattaaccc aagtcatatt ataaccgcca tccaaatgga aagaaaactc
     4021 attttcgtaa agagcacatt ctgccttacc ctcagccggg caagaccacc aactgggagt
     4081 tccattataa tcgggattac ctgcacccgg tcctacgcca aaatgtccgt catgagcacg
     4141 gtcaaatacc catgtcttac tacccgtcaa accgcctgcc aaaataatcg ctttaggttc
     4201 gatttgcgat tcatcattat tggcaatagt gatggtttgt gtaatagtcg tcgagccgcc
     4261 agtattgtat gctgtcatgg caatggtata agtgttggca aaaggataag atgccactac
     4321 ttcttcccct ttggctgtta ctccattacc taaatcccaa gtcactacaa gccctttgag
     4381 agaagatgta tttttcaaag tcaccacatt cggattactg cccggagtag cagtaaagct
     4441 caactggctt tcttgaggtt ttacacccaa tgaatggtcg tcaccatctt gtggatcaca
     4501 agaagtcatc gcaaaagcag ttactaaagc aaacagacct attttaaata tatttttcat
     4561 atcaaattct ttttaaatat tatacctgat tagtatcccg gattctgttt caattcaaac
     4621 tcagttcctt tcgttttttc aatttcattc aaaggaatag gaatgagttt cttgttatca
     4681 tcataagtac gtacagaatt atgcgcagcg tcattttccg tcaaaacagc agcagcatct
     4741 ccccaacgta ctaaatcgta aaagcgcata ccttcaccca agaattcttt atggcgctct
     4801 gatttaatgt tttcagcagt tgcagcaagc ggtttgtcaa tgccaaaagc acgtttacga
     4861 atcaagttga aacattcctg agcgtccact ccctgctgct cggctacacc gtccatcttg
     4921 accaattcca cataattcaa caatgtctca gcataacgga aaatgcgtaa attattacaa
     4981 tagttcaaat cagtatctcc gggaggagga ttataacctt cacgggctgc atatttacgt
     5041 tggaacaagc ctgtatcctg aaaacggtgt ccataactag ctcctctcca atcattaata
     5101 gaagcatcac gacgcaagtc accttcttca tacatatcat aagcagattg acgcaccgga
     5161 gcaaatcccc agccaccttt gaatactccg ctcgggtcat tcaattcatt tggagaaata
     5221 aatgcaggca gattagtacc atatccttgc cagcctgacg accatgtttt accttcaggc
     5281 aactgattgc tttcaaatat agattcttta cagaattcat tctcatccag ccacatagca
     5341 tcaaaatcat ccatcaattc aaacttgcca ctcttaataa tggtagccat gtcgttggct
     5401 atttccgcat atctgctttg atccttctga tacatcacta cacgtgcctt caacattaaa
     5461 gccgcagctc tacatgcacg tcctttattg tcaccagaat tattcaccat ttccataacc
     5521 ccctcttcac aagccacttc aaggtcagcc attatttcat tataaatttc atcggctgag
     5581 atttgcttgg tcatataagg atcttgcaaa ggctcctcga aataaggaat attcccaaag
     5641 aacttccata acaagtggac ataataagca cgcaagaagt gagcctctgc attatactgt
     5701 ttcaccttac gttcctggac accttctgca tttgcacaag ctataattgc attgttacaa
     5761 cgtgccaagc ccgtgaaata gatactccat aaacctccca ggctttcggc agcactcatg
     5821 ttgaattgtg acaaagaata taacgcagct tggtctccgg catcaccacc gcctttgtac
     5881 aaatcgtccg aacgaagatc tgacatcagc aaaatactat tataattgtt attggcataa
     5941 ctgtcaaaca aaagaatctg ataagcagat ccaagactgg aaagaatagt accttcagtc
     6001 gcaggagcgc cagccatagg cttttccgta gaatttgcgg tcaaaaaatc atcgccacat
     6061 gaagttgtca ccaatgccga tgacaaaaga gctgcgatag tgaatatttt atactttttc
     6121 atatcgtatt atttttaaga agtttagaaa gttatatttg cacctaaaga aatagttctt
     6181 gattgcggat aaataccttt atcaacgccc aaagtagtat aaccgccaga tgccagttcc
     6241 gggtcaaatc catcataacc agtaatggta atcaaatttt cggcagaaac atatactctc
     6301 aatctttgga cagagaactt ctttgtcaaa gaagcaggca gcgtatagcc caattgagcc
     6361 gttttcaagc gtaagtaaga accatctttc acatataaat cagaagaacg ccagttctta
     6421 ttaggattgg ccgaagtcat acgaggtatt gaattggaag tgccttcacc catccaacga
     6481 tcaagtatcc aagtaggacg gttcatcgcc ggaatatcac cacgctgtgc aaagtcataa
     6541 atatcatttc ctatagttcc ctggaagaac aaattcaaat cgaagccttt ccagtcagca
     6601 cctaatgtaa aaccaaatgt ccagtccggc atacctttac caatcttggt tctgtcgtca
     6661 tcattcacct taccatcacc attaaagtca acgaatctaa cgtcacccgg ttgagcatcc
     6721 ggctgcatta tttcaccttt gtcatttaca taagcgtcaa tctctgcctg attctggaac
     6781 agaccatctg tcaccatacc atagaaatag ggccatactt caccgttctt ggcgtgtaca
     6841 tagtcaccta caccagaagc acctgcattt tcatagttcg tctcaccgga tgcatttcct
     6901 aagttgatca atttattctt cagataagat gcattagcag aaatacggta actaaagtcc
     6961 ttgatggtat gtttccaacc ggcttccagc tcaatacccc agttttccat cttacccaag
     7021 ttggacatcg gagcactctg gcctacataa ctaggaatag gctggtccat caacatacca
     7081 tttgtttttt tcttaaaata atcaaagcct aaattcaggc gagcgtcaaa gaaacgtgcg
     7141 tcaaaaccca agtctatctg ttccgattct tcccatttaa tattcggatt agccaatgcg
     7201 gccggagacg aaccatattg cattacacca cccttacttg catcttttac aacataaccg
     7261 ccaccaaagt agtagttttg accgccatcc atcaaagctg cataacggaa gttaccgatg
     7321 ttttcattac cattcttacc ccagctgaaa cgaactttga aagaatcaaa ccacgaaggc
     7381 tgtatatctg ccaaatatgg ctcattccat acattccaac caacagaaac cgcagggaaa
     7441 gtagcccatt tatggtctgg tccgaaacga gaggaaccgt cacggcgcat ggttaactgc
     7501 aacatataac gttctgcgaa gttataatca atacgtccga aataagaagc caaagcagta
     7561 aagttataac cgtctgtccc cccccaaaca cgttcgtcgt cacggtcacc gatagcagaa
     7621 ttaatattag ctttgttggg atcagtatcc aacaaattat aatcagaacc acctaactta
     7681 cgtacagagt atttctgggc agattgtcct aatacaaccg tcagattgtg cttctcggca
     7741 aaagtcttgt tgtaagtcaa tacattttca acctgccatt tgaaaccgcg gttcatttca
     7801 ctttgcaccc aactcttttc atttttcttc attgagccca tataatactc aaatccgtaa
     7861 ccgtcagcgc cccaaaaagc caggtcggca ccataactgc tcttgaactt caatcccgga
     7921 agaatatcta attcagccca aaaagatcca acaaacttat cctcattatt tttagttaca
     7981 gaaggagaat tcagctgtgc aacaggattt gccaattctt gataaccggt aggaggcaaa
     8041 gagaaaacat tgccgtcctt atctgtcaca gcagtaggat attgtttcaa aatagcctca
     8101 ccttcctctt ttgaagcata aaccggcacc aatggagaaa aggcaatggc actacctaaa
     8161 atcgagccat attccgagtt tgtttcaata cctgtagaag tgcctcttga ataacctata
     8221 ttcacgccaa ctttcaactt attcaaaaat ttacgagaat tatcttcaaa tactgtataa
     8281 gttgaattag aacgcaaact ccaacgttca taattagact ttccataatc accccctaca
     8341 ataccttctt gattgaaata gcctaaagaa aggaaatact gtactttatc gctgccacca
     8401 ctaatactta cctggtgttg ctgaatagga gcatcataat tgaatgtttc atcctgccag
     8461 tcagtacctt ttccagcatt ggcaatctgt tcttgggtgt aacggggagc attaccatca
     8521 ttgacatcca tttcgttcat aatggtcata tattccgtag cgtccaaaac ggctttcttt
     8581 ttccacggat tctgccaacc gtaactgaaa tcgtaattaa tagttgtttt tcctgtttta
     8641 ccacctttcg tagtaaccaa aacaacacca ttggctgcac gcgcaccata aattgcagca
     8701 gaagcagcat ctttcaagat ttcaactgat tcaatatcaa ccgggttcag atagttaata
     8761 cccccgtcaa ccggcatgcc atcaacaata tataacgggt ctgagttatt gaccgtaccg
     8821 ataccgcgaa tacgtacttt ggaatccgaa cctggttggc cggaactttg tgtaatctgt
     8881 acaccagaca ctttaccttt caaagcatcc tctacgcgtg acggctttgc ggcgttcagt
     8941 tcatcggcac cgacacggct gatggcagca gttaccacac ttttcttttg gacaccgtaa
     9001 cccactacta caacttcatc cagtgtctct gtgtcttcca tcagcactat tttcagatta
     9061 tgctgcccct tcactgcaat gtcttgcgcc ttaaagccta tataagtaat tgaaagaact
     9121 gcatcggaag gaactgatag tgtaaattca ccctccaagc cagtgattgt accaataccg
     9181 gtatttccct tcaccaccac atttactcca ggcaatggct cattgtcaga accacttacc
     9241 accgtacctt ttacctgtat attctgggca tatacagaag ccaaggtaaa gcttaataga
     9301 aataaaactg ataataactt tttcataagt tttactaaat taagattaac atttctcggt
     9361 tttgctcata attgaacagt gcaaacatag agtaaaattg aatataacaa caaaaaacca
     9421 cctattcttt aatacgtcaa atcaaatatt caaatacgtt ataaatacgt cacaaccaat
     9481 tatactacta taaatcatac tattatatat actttagcat aatacgtcat aacatgctat
     9541 ataacacaaa aggagagaat ttaacatttc tctccttctt aaagccacaa ttacctaaag
     9601 gacgttaatt aaaaggttat agtttattat tcagataatc cgtcaagcta tcctcccttt
     9661 ccaaaccaaa tttcttgcga aggcggtaac gaatagtttc caccccacga accgaaatgt
     9721 tcaacagcgg cgcaatttct ttggtagaaa gattcatttt aagataagca cacatcatac
     9781 gttcattatt agagagatca ggatgcttgg catgaaggcg tttcataaaa ttattatgta
     9841 taagatcaaa ctgatcctca atacgcttca atacctcatc actctgaata ttagcatcaa
     9901 tcctgctgtt tataagcatc agctgctgtt tgccttcacg ggtaccctca cctttcaaca
     9961 gagttgatac cttataaatt tccgatttaa tctcagtcag catctcattc ttgcgaacta
    10021 aattaatcat cagattggcc atttcctgac ttttgtgctg caaatcatat tccaaccttt
    10081 ccttctccaa ttgcattatt tgcttttcct ggcgcgcttt ttcttcttca tacccctttt
    10141 ccaactcatg caattcttta tctttctcaa tgacagcctg ctgtttttta cgcctcacac
    10201 gaacatcatc ccaacgatag atataccaca aggcaaacaa agcaagcaca atataacaga
    10261 tatacgccgg aacactccga taccatggag ggagaatgcg aaacattatt tcatccaacg
    10321 aggtggtacc gtcgggaaaa acggctttta cctcaaaagt atattcacct tcggataaat
    10381 tactatattc ttttgtactt acagccgtaa agtcagacca attcccttta ttcaagcgat
    10441 attggaaatg tatgtcgtct ccctgcataa aggatggaag cccatactca aaacggaccg
    10501 aattctgcga atatgaaatt tgaggaaaag atttctgccc caaaaagtta gcagtatata
    10561 ccaaggagtc tttagggtaa gatagataca tgttttttat atacaaagaa tgggcatggt
    10621 cctgccgtgt ttttacagca ggaatactga aaagtgcaaa gccttcttca ttaggaataa
    10681 tcatcaaaga atcggacaaa ggaataatag cttcaaagtc cggaaccagt tccactaacg
    10741 actgttggat tggagtaata cttgtgtttg agccccgctt ataagtaccc agattggcac
    10801 tgcatatttc gtgcggactc aaactgatga gcttatcatt gtattccaat aaacgggaat
    10861 aaaccgaact gccattcaaa agattgttca tgtccggaca aggctccatt atttctttat
    10921 gaggattata tttatatata ccgctggaag tggcaaaata aatccgtcct tcaacttttg
    10981 aaacatacat atcacgtcct gccggaaatc catcatcctt gtcatactcc ttgatattga
    11041 gtactttcgt caagtcatcg gtcaactcaa tacgggttac atgatcggta ttatataccc
    11101 aaatcacctt ttcattctct tgttcaaaaa gacggcatga atctcccatt ccatctattt
    11161 tacaggccac tgtccattct ccatttttct tggctaccag ataaagaccg ttatacacac
    11221 ccacatacat taaatcagta tgccctgtca ccaactggca agaccatgca cctgtaatat
    11281 ccgtaacacg ttttaccgaa gtaccccgga tctgaaaaat gccacgatca tgcaagcaaa
    11341 acaactcatc acctatacgg caaagattcc agacttgacc actggactgg ggcattggat
    11401 taatatcagg caaattgcca ttcagtttta ccggataggg agtataatac aacccccggt
    11461 tagttcctaa ataaagatag ccgttttcca ctgcagctgt atatccggta ccgtaagaat
    11521 aaggataaga atacaaattg gtaaaaggtg aactcaagca gacgtagtca ataccactgt
    11581 ccagtccggc ccaaagattt cctgcaacat caaaggatac agataatacc gtattgttac
    11641 gcaatccgtt attctcatta aaatacttca attgcccggt actacaatct atcagcacaa
    11701 gtcctttatg aatagtgcca agcgctattt tatcatcttt ctttgccaca caaaacacct
    11761 cattctcacg catgaaatct tcggcaccag tcataaacgg ttccaccgta cggccatcac
    11821 aataatacaa accattataa gccgttacta caagaacacc ttttttataa ggtatgattc
    11881 cacggatacg tttggaagcc aatgtatccc ctccttgcaa agggaaaaat gtatttccca
    11941 ccaaaagcca tactcccctg tccgtaccca catataaaat gccattgacc atattagaac
    12001 aatcgatctt ggcatccatc tctatagcgg tatattttcc attcaagtat tttatgacac
    12061 ggtcatcacc ttgaaaatat aaaatattat ccacttcatg aataccccat acattgccaa
    12121 gaaagcggat cgagccatcc aaagtgtcgg acatgcaatg ataaaccaaa gatccgtcag
    12181 catctggctt ataatagcca aattcattaa tccctcccac ataaatacgt ttctgagcgg
    12241 cagaaggtaa aacagagcgc acatccgaag aattgttcaa cggaaaaacg ctccatacgt
    12301 tgccgtcaaa ctgtaccatg ccattcttat tggcaaaata aacccattta tcgtcataag
    12361 gggctatttg ccaggtttgc gcaccttttc cataaaggct tttatcaaaa tttacgataa
    12421 aactattcca accggcaaag agttcaacaa aaggaaaaag aaaaagccaa gaaaaaagcc
    12481 aatataattt gtgattcttc at
//
