LOCUS       NZ_KB290658            11559 bp    DNA     linear   CON 24-MAR-2023
DEFINITION  Anaerostipes hadrus DSM 3319 Scfld111, whole genome shotgun
            sequence.
ACCESSION   NZ_KB290658 REGION: 52148..63706
VERSION     NZ_KB290658.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02436796
            Assembly: GCF_000332875.2
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Anaerostipes hadrus DSM 3319
  ORGANISM  Anaerostipes hadrus DSM 3319
            Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
            Anaerostipes.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Weinstock,G., Sodergren,E., Lobos,E.A., Fulton,L., Fulton,R.,
            Courtney,L., Fronick,C., O'Laughlin,M., Godfrey,J., Wilson,R.M.,
            Miner,T., Farmer,C., Delehaunty,K., Cordes,M., Minx,P.,
            Tomlinson,C., Chen,J., Wollam,A., Pepin,K.H., Bhonagiri,V.,
            Zhang,X., Suruliraj,S., Warren,W., Mitreva,M., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2012) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            KB290658.1.
            Anaerostipes hadrus DSM 3319 is a member of the Firmicutes division
            of the domain Bacteria and has been isolated from the GI tract.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            
            The sequenced strain was obtained directly from ATCC (ATCC 29173).
            Source DNA was prepared by Michelle Daigneault and Emma
            Allen-Vercoe (Department of Molecular and Cellular Biology,
            University of Guelph, Ontario, Canada), and was funded by the NHGRI
            (through an HMP Technology Development Grant - 1R21HG005811 - 01).
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer3
            v3.02. Intergenic regions not spanned by GeneMark and Glimmer3 were
            blasted against NCBI's non-redundant (NR)  database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.1. The final gene set is processed through several programs
            such as Kegg (Release 56), psortB (Version 3.0.3) and Interproscan
            (Version 4.7) to determine possible function. Gene product names
            are determined by BER (Version 2.5). Gene names are generated at
            the contig level and may not necessarily reflect any known order or
            orientation between contigs.
            
            The National Human Genome Research Institute (NHGRI), National
            Institutes of Health (NIH) is funding the sequence characterization
            of the Anaerostipes hadrus DSM 3319 genome.
            Eubacterium hadrum DSM 3319 is a member of the Firmicutes division
            of the domain Bacteria and has been isolated from the GI tract.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            
            The sequenced strain was obtained directly from ATCC (ATCC 29173).
            Source DNA was prepared by Michelle Daigneault and Emma
            Allen-Vercoe (Department of Molecular and Cellular Biology,
            University of Guelph, Ontario, Canada), and was funded by the NHGRI
            (through an HMP Technology Development Grant - 1R21HG005811 - 01).
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer3
            v3.02. Intergenic regions not spanned by GeneMark and Glimmer3 were
            blasted against NCBI's non-redundant (NR)  database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.1. The final gene set is processed through several programs
            such as Kegg (Release 56), psortB (Version 3.0.3) and Interproscan
            (Version 4.7) to determine possible function. Gene product names
            are determined by BER (Version 2.5). Gene names are generated at
            the contig level and may not necessarily reflect any known order or
            orientation between contigs.
            
            The National Human Genome Research Institute (NHGRI), National
            Institutes of Health (NIH) is funding the sequence characterization
            of the Eubacterium hadrum DSM 3319 genome.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: High-Quality Draft
            Current Finishing Status :: High-Quality Draft
            Assembly Method          :: Velvet v. 1.1.04
            Genome Coverage          :: 103x
            Sequencing Technology    :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/24/2023 13:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,684
            Genes (coding)                    :: 2,630
            CDSs (with protein)               :: 2,630
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 15 of 54
            Pseudo Genes (incomplete)         :: 39 of 54
            Pseudo Genes (internal stop)      :: 8 of 54
            Pseudo Genes (multiple problems)  :: 7 of 54
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Anaerostipes hadrus DSM 3319"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Scfld111"
                     /strain="DSM 3319"
                     /isolation_source="stool, GI tract"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:29173"
                     /type_material="type strain of Anaerostipes hadrus"
                     /db_xref="HMP:0369"
                     /db_xref="taxon:649757"
     gene            1..981
                     /locus_tag="HMPREF0369_RS06055"
                     /old_locus_tag="HMPREF0369_01258"
     CDS             1..981
                     /locus_tag="HMPREF0369_RS06055"
                     /old_locus_tag="HMPREF0369_01258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="WP_009203603.1"
                     /translation="MNQQSKKNLTNRIVALAGITVFAFFIGIYVCVFSNYLEQHNTRK
                     KLFGATYMTMNNEFYKIVNNQIRMQVEKNGDQMITLDPALDQKKQNEQIKQLVKRKVD
                     AIFLNPVDWKAVEPGLVAAKKAKIPVIVIDSQVYRSDLVAMTIVSDNYKAGVLCAKEM
                     MKQKDQANIVLLTHNAARSAVDRINGFLDTIKNNKNYKILASADTQGQIERALPKVDK
                     IIEEYQNIDIVMGLNDPAAMGALAALDSKGAREGVLVYGIDGSPEGKKLIKEGMMTGT
                     AAQSPKQMADTAVNAAYQILQGEKVEKKKVISVQMITKKNVEQTDISRWQ"
     gene            994..2316
                     /locus_tag="HMPREF0369_RS06060"
                     /old_locus_tag="HMPREF0369_01259"
     CDS             994..2316
                     /locus_tag="HMPREF0369_RS06060"
                     /old_locus_tag="HMPREF0369_01259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="WP_008390541.1"
                     /translation="MDRKNQNLYYIKILMGVINFIILSFLSAVFILTLNKINCDFGAR
                     NFLENITYLPNEPVTSTVLIFIAFFLLLGIIEIRRKQRKRQQVSVILYAAEILLCLII
                     IKFVYVNYNGIILLVIADIVTDIKDKHTRGIFFVIMGMIYLFSDYDIISMFIDMISFQ
                     QFLSVYPAKVSMMIQGTRNILVALNAIAFIAYMIILMRNQMKENARIGLLNLQLQTAN
                     IRLKEMNEQLKDYSEMTEKMAETRERNRIAREIHDTLGHTMTGLSAGIDACIAMIDFS
                     VDATKEQLNKISQVARQGIKDIRRSVNKLRPDALEHSGLQEALEKMINETMQVSDVKI
                     NYDCQVEVLKFNQDEEDMIYRVVQESITNAIRHGKAKQIDVNLWKEDKWLNLEIKDNG
                     IGCEEIHTGFGLIHIEERIKMLKGTVEYDGSNGFKVTARIPIRWGEKL"
     gene            2313..2981
                     /locus_tag="HMPREF0369_RS06065"
                     /old_locus_tag="HMPREF0369_01260"
     CDS             2313..2981
                     /locus_tag="HMPREF0369_RS06065"
                     /old_locus_tag="HMPREF0369_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_009203604.1"
                     /translation="MIKVMIADDQELIRQSLQIVLEMKEGIEVTATAKDGREVIQEVR
                     KDKPDVILMDVRMPEMDGVQCTQIIKEQYPDVKIIILTTFDDDEYVYNALKHGASGYL
                     LKGISMDELEKAINTVHSGGAMINPEVATKVVDFFSEMAQSNYDTSIHAQNVEEITDA
                     EWEIIKLVGRGMSNKEISTELSLSEGTVRNYLSTILSKLDLRDRTQLAIWEVQSGSAK
                     YGRR"
     gene            2982..4301
                     /locus_tag="HMPREF0369_RS06070"
                     /old_locus_tag="HMPREF0369_01261"
     CDS             2982..4301
                     /locus_tag="HMPREF0369_RS06070"
                     /old_locus_tag="HMPREF0369_01261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="WP_009203605.1"
                     /translation="MRQKLEKWWKRIIAVGLVISLLSVGFVIYPKQQKIVLTFGMFAG
                     NKWDVPDDNCYQMIDQVIKEFEKEYPNVTIKYESGVMKDDYSEWLSQKALKGDLPDVF
                     MVLPEDFSTFSSVGMLKNLDSYTKIDKAFKKSNYYEGSYDAGTYKGTQYALPYESVPT
                     LMLVNKTLLKKEHIKMPGNHWTWDDFYKICQKVTKDTDGDGRLDQFGVYNYKWNDATL
                     SNGGTLFNQSGTKCNLSSEPIENAILFTKKIEKISSFRNLTSDDFDSGNIAFRPMTFS
                     EFRTYKPYPWKINKYFDFKWDCIRFPSGPDGENKTQVDNLLMGISNQTKQGDMAWQFL
                     KKLCYETKTQQKIFQYRQGISPLKAVTNSKQAQQVLEKSMGENMTDKMKLLDELMNNA
                     LQIPKFRRYNEAYQKIDSEMTRILTDEEEFDSNILKLKQEIDKFLNQ"
     gene            4463..4897
                     /locus_tag="HMPREF0369_RS06075"
                     /old_locus_tag="HMPREF0369_01262"
     CDS             4463..4897
                     /locus_tag="HMPREF0369_RS06075"
                     /old_locus_tag="HMPREF0369_01262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="WP_008390535.1"
                     /translation="MKYVVLVSHGTFAPGLHSVLKMLAGGEREDVISVGLEDGMSADE
                     FAEKFSNAISKITEDDEIILLGDIVGGSPLTNAIEQVSNKGLIGQTVIFGGMNLAMAL
                     TATLMKDGVDTDMLKDSLINEAKDAVKEFVMTSANDDEEDDI"
     gene            4918..5415
                     /locus_tag="HMPREF0369_RS06080"
                     /old_locus_tag="HMPREF0369_01263"
     CDS             4918..5415
                     /locus_tag="HMPREF0369_RS06080"
                     /old_locus_tag="HMPREF0369_01263"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_009203606.1"
                     /translation="MSVSFLRIDDRMIHGQTVTRWALEYPCDGLIAVNDKAATTDALK
                     MAYKSASTKKTFVWTYEAWKKKNQKVLDSKDNYFLITKDPIQMKKILVDDGFVPSDVK
                     KVIVGPCNDRPGAVKLGNNQSITQEEAEALEAISKAGYEIEFALIKEASIGTWDKFRG
                     QFGFN"
     gene            5442..6296
                     /locus_tag="HMPREF0369_RS06085"
                     /old_locus_tag="HMPREF0369_01264"
     CDS             5442..6296
                     /locus_tag="HMPREF0369_RS06085"
                     /old_locus_tag="HMPREF0369_01264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="WP_008390531.1"
                     /translation="MTISWLQAIILGIFACLSSMPGMGGTTIGNYTLGRPLVAGLVCG
                     IVLGDIQAGVLVGAAMQVVYIALVTPGGTVSADVRAVSYIGIPLAMLALNSYGLDPAS
                     AKGISMATSFGAMVGTIGTVLFYGTATINLVWQHIGWQAVEKGEFKRLYVVDMVLPWI
                     SHIICSFLPAVVMCKLGVPVVEAIKTTLPMDGIAMKTLFTVGSLLPCVGIAILLKQVV
                     TKAVDFIPFFFGFTLAASMGLNLVSVTVVAAMFAIIYYNIKMVGIRAKEAALASGGSF
                     DDDDEEDI"
     gene            6298..7134
                     /locus_tag="HMPREF0369_RS06090"
                     /old_locus_tag="HMPREF0369_01265"
     CDS             6298..7134
                     /locus_tag="HMPREF0369_RS06090"
                     /old_locus_tag="HMPREF0369_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="WP_009265455.1"
                     /translation="MAEKKLSKKALNKSFRNWAYGNLTCFSQEHMQTFGYLCAMLPLV
                     EELYDTKEEQKEAMNTYTAFFNTEPQVGSIIVGITAGLEEAKANGNEDIDAEAINGIR
                     AGLMGPLAGIGDSLVVGTLIPILLGIALGLSTGGSVVGPIFYIVAWNLIMAFGMKFLY
                     FKGYELGGKAVDILVGEQATAIREAIVMLGTMVVGSVAATWVSVKTSFHLVNSAGKEF
                     LNLQNQLDGVYPGFLTAAFVIFCWWLMSKKKVSPVVTMLILVVVAFIGVMIGFFNPGL
                     KY"
     gene            7150..7434
                     /locus_tag="HMPREF0369_RS06095"
                     /old_locus_tag="HMPREF0369_01266"
     CDS             7150..7434
                     /locus_tag="HMPREF0369_RS06095"
                     /old_locus_tag="HMPREF0369_01266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009203607.1"
                     /translation="MTTQTRAQQLKEIEFQTQMLNNLKKWIRNLIILSSIGIILAYWG
                     LGAQSKMPFTVFGVAGVIITIISVILCVVIGLGIKRGRANVDKILQLVKA"
     gene            7516..11559
                     /locus_tag="HMPREF0369_RS13555"
                     /old_locus_tag="HMPREF0369_01267"
     CDS             7516..11559
                     /locus_tag="HMPREF0369_RS13555"
                     /old_locus_tag="HMPREF0369_01267"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="WP_009203608.1"
                     /translation="MRKVSPRLVAIMMASVVSVTSIAPVTAYNHVTVNAAQLQQQAID
                     WTFTGNGKVDNDKIQLSGAGDNIGITNIKKEDNYAVKAKLTQGEEDGAVGVIMGAKEA
                     KDPKKGSVVANVTPSTGAVRLICFQGGRNNDLQKEKVFDELKNKDTYDFDISVKQRHL
                     TVKINGVKVISMEITDTIFEGKVGLISFNETMTASDFSVEDIAIPKATAHLTNLQVTG
                     GDLDKKYTQDTNDYAITVPNTVDKLSITPTVSDNGTVTIGEQKAENGKVTEVSLKIGS
                     QEIPITVTSTNGAVETTTLHVLRNVDGSAYTVPTRPQYHFSQAMGWSNDPNGMLYYKG
                     EWHLFFQHNQARTTWGELEWGHAVSKDLIHWEELPRPLNYQEDNGAMFSGCGVVDDEN
                     TSGLFGDEKGPGKGGLIALYTQNNELNGHRGQDQCIAYSKDNGRTWTKYKGNPILKWD
                     EDHLKDTAFRDPKVFRHDGKWFMVLAGGILRIYSSDDLIHWNIESTYKGSDDIGNPAG
                     LRVETECPDMYPLTADDGTTKWVISEGGRYYRIGDLKKIDGHWTFVQDKDSDRYVMNF
                     GPHSYAAMTYYIGNGETTNGKPENRRIMINWASTWADGYCNNVDKVTGQWGYNGFFNL
                     QTELNVKKIDGKYKLVQTPIDEYKTLRVNEAATKLENVTIPKKTENSENLLSGVKAGQ
                     YEVVAELTPQAGTKEVGFKLRTNKSGSQETVVSYNTETKKVSINGEKSGTHPAGQQTK
                     NIVSDAIVTEKDGKVKLDIFVDESSVEVYGQDGQVTGALAVFPSVSSTGMEVYSEGGE
                     TTGNITVYPMKSIWENKITDANTATDLYISTDSGSGEYNVGDKISVNAAISPMKADQK
                     VTWKVSNNKSNKVKVVKTEDDELQLEALKEGSVTVTATTANGLSRSIDIFVSAADDGI
                     SLSDWKQHGGTWTISENSNSCTGEASGDAFLMSGTKISSDDYIFESDVVYHSGQAFAL
                     LFRGQNPDSTSAYAANVDTQRKGSTARIFTFGGGTGDIGKDGNYNLSEGQKYHFKVVV
                     KGNQYKVYIEDKLVLNVRDVKVENNYKEGKYVGFNVFNGKVTFQNMKVTPLEVKVPTV
                     SDKLALTAGEKATIDVSNIEDNSFNIIKYKSLDKTIADVDEDGNITAKKAGTTQIETQ
                     VTTFGRSYTYKTQVTVKEKEVETPKVSITATLKGNAGKTVTTIKDVKAGDTLPAWNKA
                     KYKAAGKKGYVFAGWTYNGKIVTKMPESDKNITLTAKFVKVTVGKAGSLSVRSKAGKG
                     VGFVARSTQSVNKYGEKRGFRFRYSTSKKMKSAKYKTTGLAKNVYTKTGLKKGKKYYV
                     QVRYYYYDSTNQKVYGAYSNAKSVKAN"
ORIGIN      
        1 atgaaccaac aatcaaaaaa aaatctaacc aataggattg tcgctttagc aggaatcaca
       61 gtttttgctt tttttattgg aatttatgtc tgtgtatttt caaactattt ggaacagcat
      121 aacacaagaa aaaaattatt cggtgcaact tatatgacga tgaataatga attctataag
      181 atagttaata accagattcg gatgcaggtt gagaagaatg gggaccagat gatcacatta
      241 gatccagcac tcgatcagaa gaagcagaat gaacagatta aacagttggt caaacgaaaa
      301 gtagatgcga tttttctaaa tccagtagac tggaaagcag tagaaccagg acttgtagct
      361 gcaaagaaag cgaagattcc cgtgattgtt atcgactcac aggtttacag aagtgatctc
      421 gtcgcaatga cgatcgtgtc agataactac aaggcaggcg ttttatgtgc caaggaaatg
      481 atgaagcaaa aagatcaggc gaatatcgtc ttgttaacac ataatgcagc aagatctgcg
      541 gtagatcgta tcaatggatt tttagatacg atcaagaaca ataaaaacta taagattctt
      601 gcatctgcag atactcaagg acagattgaa cgtgcacttc ctaaggtaga taagattatc
      661 gaagaatacc agaacataga tattgtcatg ggattaaatg acccagcggc gatgggagct
      721 ttggcggctc ttgacagcaa gggtgcaaga gaaggggttc ttgtatacgg aatcgatggt
      781 tcgccagaag ggaaaaagct gatcaaagaa ggtatgatga caggaacagc ggcacaatct
      841 cccaaacaga tggcagacac agcggtcaat gcggcatacc agattctgca aggggagaaa
      901 gtagagaaga aaaaggtaat ctctgttcaa atgataacaa aaaagaatgt agagcagact
      961 gatattagcc gttggcagta aagaggaaat gatatggatc gaaaaaatca aaatttatat
     1021 tatatcaaga ttctgatggg agtcataaat ttcatcattt tatccttcct ttctgcagtt
     1081 tttattttaa cattaaataa gatcaattgt gattttggag caaggaattt cttagagaat
     1141 atcacatatc tcccaaacga accagtgaca tcaacagtgt tgatatttat tgcttttttc
     1201 ttattgttag gcattataga aattcgaaga aaacaaagaa aaaggcaaca ggtatctgtg
     1261 atcttatatg cagcagagat tttgttatgt ctgatcatta ttaaatttgt ttatgtaaat
     1321 tataatggaa tcattcttct tgtcattgca gatattgtaa cggatatcaa agataaacac
     1381 actaggggaa ttttctttgt catcatggga atgatatatt tattctcaga ctacgatatc
     1441 atatcaatgt ttatagatat gatatcgttc caacaatttt tgagtgtata cccggcgaaa
     1501 gtcagtatga tgatccaagg aacaagaaat atactagttg cattgaatgc gatcgcattt
     1561 attgcataca tgatcatttt gatgagaaat cagatgaaag agaatgcaag gattggattg
     1621 ttaaaccttc agcttcagac agccaatata cgtttaaaag aaatgaatga acagctgaag
     1681 gactattcag agatgacaga gaaaatggcg gagacaaggg aacgaaatcg aattgccaga
     1741 gagattcatg acacactggg gcatacgatg actggactgt ctgcaggaat tgacgcatgt
     1801 atcgcgatga tcgatttttc tgtagatgca acgaaagaac agttaaataa aatatcacag
     1861 gttgcaaggc aaggaattaa agatattcga agatcggtca ataaattaag accagatgcc
     1921 ttagagcata gtggattgca agaagcttta gaaaagatga tcaatgagac gatgcaggta
     1981 tcagatgtta agattaatta tgattgtcag gtagaggtct tgaaattcaa tcaagatgaa
     2041 gaagatatga tctatcgtgt agtacaggaa agtattacaa atgcgatcag gcatggaaaa
     2101 gcgaagcaga ttgatgtaaa cttatggaag gaagataagt ggctcaatct agagatcaaa
     2161 gacaatggaa tcggatgtga agagattcat acaggatttg gattgatcca tatcgaagag
     2221 aggatcaaga tgttaaaagg aactgtagaa tatgatggaa gtaatggatt taaagtcaca
     2281 gccagaatac cgatcagatg gggtgaaaaa ctatgattaa agtaatgata gcagatgatc
     2341 aggaactgat cagacaaagt ttacagatcg tacttgagat gaaagaagga atcgaagtta
     2401 ctgcaactgc caaggacgga agagaagtca ttcaggaagt caggaaagac aagccagatg
     2461 ttattttaat ggatgtgcgt atgccagaaa tggatggagt gcaatgtaca cagatcatca
     2521 aagaacaata tccagatgtc aagatcatta ttttgacaac gttcgatgat gatgagtatg
     2581 tatacaatgc attaaaacat ggagcaagtg gttatctgtt aaaaggaatt tctatggatg
     2641 aattagaaaa agcgatcaat acagtacata gcggcggggc catgatcaat ccagaagtgg
     2701 caacaaaggt ggtcgatttc ttctctgaaa tggcacaaag caattatgat acatcaattc
     2761 atgcacagaa tgtagaagag atcacagatg cagaatggga gatcattaaa ttagtcggaa
     2821 gaggcatgtc aaataaagaa atctcgacgg aactaagcct ttccgaagga actgtacgaa
     2881 attatctcag cacgatctta agtaaactag acctaagaga cagaacccag cttgcaatct
     2941 gggaagtaca gtcaggttca gccaaatacg ggagaagata gatgagacag aaactagaaa
     3001 aatggtggaa acgaatcatt gctgttggat tggtcataag tttattatct gttggttttg
     3061 tgatctatcc caaacaacaa aaaattgttt taacctttgg aatgtttgca ggaaacaagt
     3121 gggatgttcc agatgataat tgctatcaga tgatcgatca agtaatcaaa gaatttgaaa
     3181 aagaatatcc gaatgtcacg atcaaatacg aaagtggtgt gatgaaagat gattattcag
     3241 aatggctgtc acaaaaggca ctaaaaggag atctgccaga tgtttttatg gtcttgccag
     3301 aagattttag cacattttca tccgtaggaa tgttaaagaa cttagactcc tatacaaaga
     3361 ttgataaagc gttcaaaaaa agcaactatt atgaaggaag ttatgatgct ggaacctata
     3421 agggaacgca atatgctctt ccatacgaaa gtgtaccaac tttgatgcta gtcaataaga
     3481 cactactaaa aaaagagcat atcaaaatgc cagggaatca ctggacttgg gatgattttt
     3541 ataagatctg tcaaaaagta accaaagata ccgatggaga tggccgttta gaccaatttg
     3601 gtgtatataa ttataaatgg aatgatgcaa ctctgagcaa tggcggaaca ttgtttaatc
     3661 aaagtggaac aaaatgcaac ttgtcatccg aaccaattga aaatgcaatc ctctttacaa
     3721 agaagattga aaaaatatca tcttttcgaa atctgacatc tgatgatttt gattctggaa
     3781 atattgcatt tcgtcctatg acattttcag aatttagaac gtataaacca tatccatgga
     3841 agatcaataa atactttgat ttcaaatggg attgtatcag attcccttcc ggaccagatg
     3901 gagagaataa gacacaggta gataatctgc tgatgggaat cagcaatcag accaagcaag
     3961 gagatatggc atggcagttt ttaaagaaat tatgttatga aacaaagaca cagcaaaaga
     4021 tctttcagta tcgtcaggga atctcaccat taaaagctgt gacgaattca aaacaagcac
     4081 agcaggtatt agaaaaaagc atgggtgaga atatgacaga taaaatgaaa ttgttagatg
     4141 aactcatgaa caatgccttg cagattccaa aattccgaag atacaatgaa gcctaccaaa
     4201 agattgacag tgagatgaca agaattctta cagatgaaga agaatttgat tccaatatct
     4261 tgaaactaaa acaagagatt gataagtttt taaatcagta acaataagaa aatcaaaaca
     4321 aataaaacac gaatatgaca tttgtcacat aaaaaaggac atttgtaaat tgaaagtcat
     4381 gacaatttac agatgtcctt tttttacatg cttggtagaa taagtttgta aataagagat
     4441 aggaataaga aaggagaaga ggatgaaata cgtagtatta gtcagccacg gaacatttgc
     4501 accaggactt cacagtgtac ttaagatgtt agctggtgga gaaagagaag acgtgatcag
     4561 cgtcggatta gaggacggaa tgagtgctga tgaattcgca gagaaattca gcaatgcgat
     4621 cagcaagatc acagaagacg atgagatcat tcttcttggt gacattgtgg gtggatcacc
     4681 tcttacaaat gcaattgaac aggtatcaaa taaaggactg atcggtcaga cagtgatctt
     4741 tggtggaatg aacctggcaa tggcattgac agcaacatta atgaaagacg gcgtagacac
     4801 agacatgcta aaagacagct tgatcaatga ggcaaaagat gcagttaaag aatttgttat
     4861 gacatcagca aacgatgatg aagaagacga tatttaaatt ttaaggagga aaaaaatatg
     4921 tcagtttcat ttttaagaat tgatgaccgt atgatccatg gtcagacagt aacacgttgg
     4981 gcactagagt atccatgcga tgggctgatc gcagtgaacg acaaagcagc aacaacagat
     5041 gcacttaaga tggcatacaa gagtgccagc acaaagaaaa catttgtttg gacatatgaa
     5101 gcatggaaaa agaaaaacca gaaagtatta gacagcaaag acaattactt cctgatcaca
     5161 aaagatccaa tccagatgaa gaagatctta gtggatgatg gatttgttcc aagtgatgta
     5221 aagaaagtaa tcgtaggacc atgtaatgac agaccgggag ctgtcaaact tggtaataac
     5281 caatctatta cacaggaaga agcagaagct ttagaagcga tcagcaaagc tggttatgaa
     5341 atcgaattcg cactgatcaa agaagcgtcc atcggaacat gggacaaatt cagaggtcag
     5401 tttggattta actaaaaaac caatagagag gagaaactat tatgacaatt agttggttac
     5461 aagcaatcat tttaggtatt tttgcctgct tatccagtat gcctggtatg ggaggtacaa
     5521 ccattggtaa ctacacatta ggaagaccat tagttgcagg tcttgtctgt ggtatcgtac
     5581 ttggagatat tcaggcaggt gttttagtcg gtgcggcaat gcaggttgta tatatcgcat
     5641 tggttacacc aggaggaact gtatcagcag acgttcgtgc cgtatcttat atcggtattc
     5701 cactggcaat gttagcatta aattcatacg gattagatcc tgcatcagca aaaggtatct
     5761 ccatggcaac atcatttgga gcaatggtag gaacaatcgg tacagtatta ttctatggaa
     5821 cagcaacgat caaccttgta tggcagcaca ttggatggca ggccgttgaa aaaggtgaat
     5881 tcaagagatt atacgttgta gatatggttc ttccatggat ttctcatatc atctgcagct
     5941 tcttaccagc agttgtaatg tgtaaattag gagttccagt tgtagaagcg atcaaaacaa
     6001 cattaccaat ggatggaatt gcaatgaaaa cactgtttac agtaggtagc ttacttcctt
     6061 gcgtaggtat tgcgatctta ttaaaacagg tagtaacaaa agcagttgac tttataccat
     6121 tcttcttcgg atttacatta gcagcaagta tgggattaaa ccttgtatca gtaacagttg
     6181 tagcagcaat gttcgcaatt atttactaca acattaaaat ggtaggaatc agagcaaaag
     6241 aagcagcctt agcttctggc ggttcctttg acgatgatga cgaggaggat atttaatatg
     6301 gcagagaaaa aactatctaa aaaagcgtta aataaatcat tccgtaactg ggcttatgga
     6361 aacttaacat gtttctctca ggaacatatg cagacatttg gttatctgtg tgcaatgctt
     6421 ccattggtag aagagttata tgatacaaaa gaagaacaga aagaagcaat gaatacatac
     6481 acagctttct ttaatacaga accacaggtt ggatctatca tcgtaggtat cacagctggt
     6541 cttgaggaag caaaagcaaa tggaaatgaa gatatcgatg cagaagcaat caatggtatc
     6601 cgtgccggtc ttatgggacc tttagcaggt attggtgatt ccttagttgt aggtacatta
     6661 attcctatct tacttggtat cgcactggga ttatcaacag gtggatctgt cgtaggaccg
     6721 atcttctata tcgtagcatg gaacttgatc atggcatttg gaatgaagtt cttatacttc
     6781 aaaggatatg aactgggtgg aaaagccgta gatatcctgg ttggtgagca agcaaccgcg
     6841 atccgtgaag caatcgttat gttaggaaca atggttgttg gatctgtagc agcaacatgg
     6901 gtatcagtaa aaacatcatt ccatcttgta aacagtgcag gaaaagaatt cttaaatctt
     6961 cagaatcagt tagacggagt atatccagga ttcttaacag cagcttttgt aatcttctgt
     7021 tggtggttaa tgtcaaagaa aaaagtatct ccagttgtta caatgttgat cttagtagtt
     7081 gtagcattta tcggagttat gatcggattc tttaacccag gattaaaata ctaagaaagg
     7141 aagtatatta tgacaacaca aacaagagca cagcagttaa aagaaattga atttcagaca
     7201 caaatgctca ataacctcaa aaaatggatt cgcaatctca ttattctatc ttctatcgga
     7261 atcatacttg cgtattgggg actaggtgcc caaagtaaaa tgccatttac agtatttggt
     7321 gtagctggag tgatcattac gatcatcagt gtgatacttt gtgttgtcat aggccttggt
     7381 attaagcgag gaagagcaaa cgtagacaag atattacaat tagtgaaagc ctaagcatag
     7441 aactattgtt ttttgagtga aaaaggacga ttatagttag atcgtccttt ttttctacaa
     7501 agaaagggaa aaaatatgag gaaagttagc ccaagattag tagcaataat gatggcaagt
     7561 gtagtcagtg tcacttctat cgcgccagta acagcataca atcatgtaac tgtaaatgca
     7621 gcacaattac agcagcaggc gatagattgg acatttacag ggaatggaaa agtagacaat
     7681 gataagatcc agttatcagg agctggtgat aatatcggta tcacaaacat caaaaaagaa
     7741 gataattatg cggtcaaagc aaaactgaca caaggagaag aagacggagc tgtcggtgtg
     7801 attatgggag caaaggaagc aaaagatcca aagaaaggtt ctgtagtagc caatgttact
     7861 ccatctactg gagctgtacg tttgatctgt ttccaaggag gacgcaacaa cgatcttcag
     7921 aaagaaaaag tatttgatga attaaaaaat aaagatactt atgattttga catttctgta
     7981 aaacaacgtc atttaacagt taagatcaat ggtgtaaaag tcatcagtat ggagatcaca
     8041 gatacaattt ttgaaggaaa ggttggtctt atttccttca atgaaacaat gacagcatct
     8101 gatttttctg tagaagatat cgcgattcca aaagcaacag cacatttaac aaatttacag
     8161 gtaacaggtg gcgacttaga taagaaatat acacaggata caaatgatta tgcgatcaca
     8221 gtgcctaata cagttgataa attatctatc acaccaactg taagtgataa tggaacagtg
     8281 acgatcggtg aacaaaaagc agaaaatgga aaagtaacag aagtatcatt aaagattgga
     8341 tctcaggaaa ttcctatcac agtgacaagt acaaatggtg cagtagaaac tacaacatta
     8401 catgtattaa gaaatgtaga tggttcagca tatacagtgc caaccagacc acaatatcat
     8461 ttttcacagg caatgggatg gagcaatgac ccaaacggaa tgctttatta taagggagaa
     8521 tggcatttat tcttccagca taatcaggca agaacgacat ggggagagtt agaatgggga
     8581 catgcagttt ctaaagatct aatccactgg gaagaattgc caagaccatt aaattatcag
     8641 gaagataatg gagcaatgtt ctctggatgt ggtgttgtag atgatgagaa tacatccggt
     8701 ttatttggag atgaaaaagg acctggaaaa ggtggactta tcgcgctcta tacacagaac
     8761 aatgaattaa atggccacag aggacaggat cagtgcatcg catatagcaa agataacgga
     8821 agaacatgga caaaatataa aggaaatcca atcttaaaat gggatgaaga tcatctaaaa
     8881 gatacagcat tccgtgatcc aaaagtattc cgccacgatg gaaaatggtt tatggtatta
     8941 gcgggaggaa tcttaagaat ttattcttct gatgatctga ttcattggaa tattgaaagt
     9001 acatataaag gaagtgatga tatcggtaat ccagcaggat tgagagttga aacagaatgt
     9061 cctgatatgt atcctttaac agcagatgat ggaacaacaa aatgggttat cagtgaagga
     9121 ggacgttatt atcgcatcgg tgatctcaag aagattgatg gacattggac atttgtacag
     9181 gataaagaca gcgaccgtta tgtcatgaac tttggacctc attcttatgc agcaatgaca
     9241 tattatattg gtaatgggga aacaaccaat ggaaaaccag aaaaccgaag aatcatgatc
     9301 aactgggcaa gtacatgggc tgatggatac tgcaataacg ttgataaagt gactggacag
     9361 tggggataca atggattctt taatcttcag acagaattaa atgtgaagaa gattgatggt
     9421 aaatataaac tggtacagac accaattgac gaatataaga cgttacgtgt caacgaagca
     9481 gcaaccaaac ttgaaaatgt tacgattcca aagaagacag aaaacagcga aaatctgtta
     9541 tcaggagtca aagcaggaca atatgaagtt gtcgcagaac ttacaccaca ggcaggaaca
     9601 aaagaagttg gattcaaatt aagaacaaac aaatctggaa gtcaggaaac agttgtatct
     9661 tataatacag aaacaaagaa agtatccatc aatggagaaa aatctggtac acatccagcg
     9721 ggacagcaga caaagaacat cgtatctgat gcgatcgtca cggagaaaga tggaaaagta
     9781 aaattagata tctttgttga tgaatcaagt gttgaagtat atggacaaga tggacaggtt
     9841 acaggagcat tagcagtatt cccatctgtc agtagtacag gaatggaagt atattctgaa
     9901 ggtggagaaa caacaggaaa tatcacagtt tatccaatga agagtatctg ggaaaataag
     9961 atcacagatg caaacacagc aacagatcta tatatcagta cagactctgg aagtggagaa
    10021 tataatgtcg gagataagat tagtgtaaat gctgcaatct ctccaatgaa agcagatcag
    10081 aaagtaacat ggaaagtaag taataacaaa agtaataaag ttaaagttgt taaaacagaa
    10141 gatgatgaac ttcagttaga agcactaaaa gaaggaagtg tcactgttac agcaacaaca
    10201 gcaaatggat tatcaagaag tattgatata tttgtttcag cagcagacga tggtatttca
    10261 cttagtgact ggaaacaaca tggtggtaca tggacgatca gtgagaattc taacagttgt
    10321 acaggagaag ccagtggaga cgcattctta atgtcaggaa caaagatcag ttcagatgat
    10381 tacatttttg aatcagatgt tgtttatcac agtggacagg catttgcatt attattccgt
    10441 ggacagaacc cagattctac cagtgcatat gcagctaatg tagacacaca aagaaaaggt
    10501 agtacagcaa gaatctttac atttggaggc ggaaccggag acatcggaaa agatggaaat
    10561 tacaacttaa gcgaaggaca gaaatatcat ttcaaagttg tagtaaaagg taaccagtat
    10621 aaagtgtata ttgaggacaa gctggtactg aatgtaagag atgtaaaagt tgagaataat
    10681 tacaaagaag gaaaatatgt tggatttaat gtattcaatg gaaaagtaac attccagaat
    10741 atgaaagtca caccattaga agtgaaagtt ccaacagttt ctgataaact tgcattaaca
    10801 gcaggcgaga aagcaacaat tgatgtttcc aatatcgagg acaacagttt caacatcatc
    10861 aaatacaaat ctttggacaa aacgatcgca gatgtagatg aagatggtaa catcacagca
    10921 aagaaagctg gaacaacaca gattgaaacg caggttacaa cttttggaag aagttacaca
    10981 tacaagactc aggtaacagt aaaagaaaaa gaagtagaaa ctccaaaagt ttctattaca
    11041 gcaacactaa aaggtaatgc aggaaagact gtcacaacga tcaaagatgt gaaagcagga
    11101 gatactctgc cagcatggaa caaagcaaaa tataaagcag ctggtaagaa aggttatgta
    11161 tttgcaggat ggacatacaa tggaaaaatt gttacaaaga tgccagaaag cgacaagaat
    11221 attacattaa cagctaaatt tgttaaagta acagttggaa aagcaggttc attatctgtc
    11281 agatccaaag caggaaaagg tgtaggattt gtagcaagat caacacaatc tgtcaacaaa
    11341 tatggagaaa aacgaggatt tagattccgt tattccacaa gcaaaaagat gaaatctgca
    11401 aaatacaaaa caacaggttt agcgaaaaat gtttatacaa aaacaggtct aaaaaaaggc
    11461 aagaaatatt atgtacaggt tcgctattac tactatgatt caacaaatca gaaagtatac
    11521 ggagcatatt ctaatgcgaa gtcagtgaaa gcaaattaa
//
