LOCUS       NZ_KB290636             7838 bp    DNA     linear   CON 24-MAR-2023
DEFINITION  Anaerostipes hadrus DSM 3319 Scfld13, whole genome shotgun
            sequence.
ACCESSION   NZ_KB290636 REGION: 72733..80570
VERSION     NZ_KB290636.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02436796
            Assembly: GCF_000332875.2
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Anaerostipes hadrus DSM 3319
  ORGANISM  Anaerostipes hadrus DSM 3319
            Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
            Anaerostipes.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Weinstock,G., Sodergren,E., Lobos,E.A., Fulton,L., Fulton,R.,
            Courtney,L., Fronick,C., O'Laughlin,M., Godfrey,J., Wilson,R.M.,
            Miner,T., Farmer,C., Delehaunty,K., Cordes,M., Minx,P.,
            Tomlinson,C., Chen,J., Wollam,A., Pepin,K.H., Bhonagiri,V.,
            Zhang,X., Suruliraj,S., Warren,W., Mitreva,M., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2012) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            KB290636.1.
            Anaerostipes hadrus DSM 3319 is a member of the Firmicutes division
            of the domain Bacteria and has been isolated from the GI tract.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            
            The sequenced strain was obtained directly from ATCC (ATCC 29173).
            Source DNA was prepared by Michelle Daigneault and Emma
            Allen-Vercoe (Department of Molecular and Cellular Biology,
            University of Guelph, Ontario, Canada), and was funded by the NHGRI
            (through an HMP Technology Development Grant - 1R21HG005811 - 01).
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer3
            v3.02. Intergenic regions not spanned by GeneMark and Glimmer3 were
            blasted against NCBI's non-redundant (NR)  database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.1. The final gene set is processed through several programs
            such as Kegg (Release 56), psortB (Version 3.0.3) and Interproscan
            (Version 4.7) to determine possible function. Gene product names
            are determined by BER (Version 2.5). Gene names are generated at
            the contig level and may not necessarily reflect any known order or
            orientation between contigs.
            
            The National Human Genome Research Institute (NHGRI), National
            Institutes of Health (NIH) is funding the sequence characterization
            of the Anaerostipes hadrus DSM 3319 genome.
            Eubacterium hadrum DSM 3319 is a member of the Firmicutes division
            of the domain Bacteria and has been isolated from the GI tract.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            
            The sequenced strain was obtained directly from ATCC (ATCC 29173).
            Source DNA was prepared by Michelle Daigneault and Emma
            Allen-Vercoe (Department of Molecular and Cellular Biology,
            University of Guelph, Ontario, Canada), and was funded by the NHGRI
            (through an HMP Technology Development Grant - 1R21HG005811 - 01).
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer3
            v3.02. Intergenic regions not spanned by GeneMark and Glimmer3 were
            blasted against NCBI's non-redundant (NR)  database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.1. The final gene set is processed through several programs
            such as Kegg (Release 56), psortB (Version 3.0.3) and Interproscan
            (Version 4.7) to determine possible function. Gene product names
            are determined by BER (Version 2.5). Gene names are generated at
            the contig level and may not necessarily reflect any known order or
            orientation between contigs.
            
            The National Human Genome Research Institute (NHGRI), National
            Institutes of Health (NIH) is funding the sequence characterization
            of the Eubacterium hadrum DSM 3319 genome.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: High-Quality Draft
            Current Finishing Status :: High-Quality Draft
            Assembly Method          :: Velvet v. 1.1.04
            Genome Coverage          :: 103x
            Sequencing Technology    :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/24/2023 13:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,684
            Genes (coding)                    :: 2,630
            CDSs (with protein)               :: 2,630
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 15 of 54
            Pseudo Genes (incomplete)         :: 39 of 54
            Pseudo Genes (internal stop)      :: 8 of 54
            Pseudo Genes (multiple problems)  :: 7 of 54
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Anaerostipes hadrus DSM 3319"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Scfld13"
                     /strain="DSM 3319"
                     /isolation_source="stool, GI tract"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:29173"
                     /type_material="type strain of Anaerostipes hadrus"
                     /db_xref="HMP:0369"
                     /db_xref="taxon:649757"
     gene            1..981
                     /locus_tag="HMPREF0369_RS01715"
                     /old_locus_tag="HMPREF0369_00355"
     CDS             1..981
                     /locus_tag="HMPREF0369_RS01715"
                     /old_locus_tag="HMPREF0369_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_009202970.1"
                     /translation="MAKKMTMADIANLSGVGKSTVSRYFNGGYVKDETRAKIQKVIEE
                     YNYTPNAFARLNAKQSNVIGVVVPTLNSKITSRVITSIDRYLREKGYTTLIQNSDHDI
                     DQELQNIQRLIQLNVDGILISSIAITKDHKELLKKAGIPVVVLTQDYEDGISIIYDDY
                     HAGKTIGEYIGKKGHQKVGYIGVYETDQAVGIERRNGVLDGLKEYGITPVTGKSDYSF
                     IGGQMVTRELLEMESDLDAIICATDRLAFGAYKILHEHKKRIPEEVSVAAFGGYDEST
                     LLTPELTTLKFDSYGMGYLGAETILKMITGEPVSKKQIVGYEFIEGGSVR"
     gene            complement(1393..2553)
                     /locus_tag="HMPREF0369_RS01720"
                     /old_locus_tag="HMPREF0369_00356"
     CDS             complement(1393..2553)
                     /locus_tag="HMPREF0369_RS01720"
                     /old_locus_tag="HMPREF0369_00356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_040343861.1"
                     /translation="MRKLLKSFKTEINPTVEQKIKINKTIGTCRYVYNFYLGHNKALY
                     DKGEKFMTGKSFSVWLNNEYIPNNPEKVWMKEAYSKAVKKSIEDGCTAFTRFFKHQSA
                     FPNFKKKGKSDVKMYFVKNNPKDCRCERHRLNIPSLGWVRMKEKGYIPTTKDGWKIKS
                     GTVSIKAGKYYVSVLVEIPDAKIANNSNDGIGIDLGLKDLAIVSNGKTYKNINKSARV
                     KKLEKKLRREQRCLSRKYENLKKGESTQKNIQKQKLKVQRLYHKIDNIRTDYINKSIA
                     EIVKAKPSYITIEDLNVSGMMKNRHLSKAVASQKFYEFRTKLKAKCDENGIELRVVDR
                     WYPSSKMCHCCGAIKKDLKLSDRIYRCDCGYVENRDFNAALNLRDALTYEVA"
     gene            complement(2465..3139)
                     /locus_tag="HMPREF0369_RS01725"
                     /pseudo
     CDS             complement(2465..3139)
                     /locus_tag="HMPREF0369_RS01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            3448..5373
                     /locus_tag="HMPREF0369_RS01735"
                     /old_locus_tag="HMPREF0369_00358"
     CDS             3448..5373
                     /locus_tag="HMPREF0369_RS01735"
                     /old_locus_tag="HMPREF0369_00358"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202973.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="WP_009202973.1"
                     /translation="MDYAKIASQVIEYVGGKQNIKSVQHCATRLRLQLKDNDLRNEDA
                     VSDIEGVKGVFLTQSQFQIIFGSGLVNLVCDEVQKQLGISVDTPADDEKEEKKGNVLQ
                     RLVKLLSDIFVPIIPAIVAGGLLMGVNNILTAAMFSGKSVIDLYPQFKGLATAINMFA
                     SAPFAFLPVLIGFSATKKFGGNPYLGAAMGMIMVHPDLLSAYSIGIAKVPVWDIFGFK
                     IQAIGYQGTVLPVLAVAFILATIEKKLHKVTPTWLDNLTTPLISIMVTSFLTFICVGP
                     VLREAGNLLADGITWVYNTLGFVGGGLFGLAYAPICLTGMHHSFIAIETQLIAAKATT
                     GGSFIFTTASMNNVAQGAAVLAVLFLTKNEKMKSICSASGVSALLGITEPAMFGVTLK
                     LKYPFYAAIIGSAVGNAYCAATGVLAQALGAAGLPGFLSMLPKDYLNFVIGLLLSMGV
                     SAVLTAVFWKKFNVEGEDHKAETPVKEEVKEIEVQEPADTTEETNELYAPMKGEVLDV
                     AKSADPAFASKAMGEGVAINPSEGIVYAPADGTISLIFPTKHAMGITLNSGVELLIHA
                     GIDTVKMNGEGFETFVETGAKVKKGDKLLSFDMDLVKEKGYKTQTMFLVADAKGKEVE
                     VVEAADADNNTKIMNLK"
     gene            5408..6874
                     /locus_tag="HMPREF0369_RS01740"
                     /old_locus_tag="HMPREF0369_00359"
     CDS             5408..6874
                     /locus_tag="HMPREF0369_RS01740"
                     /old_locus_tag="HMPREF0369_00359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_009202974.1"
                     /translation="MFEKYKKVYKADYEKWYKENEAYRERVAADQDRLKFHLMPKTGW
                     LNDPNGLCQFNGTYHIYYQYTPFEPTGELKTWGHYTTKDFIHYEDFGPVVFPDEDIDA
                     HGVYSGSAFIEDDTIHYFYTGNLKYFDRDDYDYINSGRGSNTITFTSKDGYEFTKKEL
                     LMTTDDYPADMSNHVRDPKIFKKNEKYYMVLGARDVEGVGMILLYESTDLKNWTYKNR
                     ITTPQKFGYMWECPDLFEMDGQLYIICCPQGVETQGIDYENVHQVTAMKLDYDFDTDE
                     YEITDIKLFDRGFDFYAPQSFEDESGRRILIGWMGIPDADYTNPTEEAGWQHALTIPR
                     ELSVRDGKLIQEPIEELKQLRSEDKAVCTFCYGQTIIMQNNIYEAVVDFKRCREMVMT
                     LREGITLSYKDNILTLNLGVYGSGRSTRKVRLEKLEHLQIFADTSSLEIFVNHGEEVF
                     TTRIYNHHGRLLINGECSGIMTVYPLKSFEIEGGRHEE"
     gene            6864..7838
                     /locus_tag="HMPREF0369_RS01745"
                     /old_locus_tag="HMPREF0369_00360"
     CDS             6864..7838
                     /locus_tag="HMPREF0369_RS01745"
                     /old_locus_tag="HMPREF0369_00360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="WP_008394375.1"
                     /translation="MKNKLCGIGEALIDFIPEVKGQRLKDVPSFKRVAGGAPANVVGA
                     VTKLGIPSKFLTKLGDDPFGDYIVEVLDEAGIDTSNIARDKEGETALAFVSLASDGNR
                     DFKFYRKNSADLRYSVEDIPADILNDCGMIHFCSVDLVESPMKEAHKKLIDMAIAQNV
                     KVSFDPNLRFSLWDDLDQLKETVNDFLKYADIIKISDEELEFITGHTDIKDALDGLFA
                     DRAKYVIYTKGADGAEVYTRNGMVEASGYKIDVRDTTGAGDSFIGAFLYCLLNDEVED
                     LESVSDDTLRNYLDFANAYAANTTTKEGALAAMADRAEMDEWMKTLKK"
ORIGIN      
        1 atggcaaaga agatgaccat ggcagacatc gcgaatttat caggagttgg aaaaagcacg
       61 gtatctcgct attttaacgg gggatatgta aaggatgaga cgagagcgaa gattcagaag
      121 gtcattgaag aatacaacta tacaccgaat gcatttgcaa gattgaatgc gaaacaaagc
      181 aacgtgatcg gtgtagttgt gccgacatta aattctaaga tcacaagtcg tgtgatcaca
      241 agtatcgatc ggtatttaag agaaaagggg tatacaaccc ttattcagaa ctcagaccac
      301 gatattgatc aggaattaca gaatatccag cgcctgatcc agttaaatgt ggatgggatc
      361 ttgatcagtt ccatagcgat cacaaaggac cacaaagagc tgttaaagaa agcggggatt
      421 ccagtggttg ttttaacaca ggattatgaa gatggaattt ccattattta tgatgattat
      481 catgcaggga aaacgatcgg agagtatatc gggaagaaag gacatcaaaa ggttggatat
      541 attggtgtct atgagacaga ccaggcagtt ggaatcgagc gtagaaatgg tgtgttagat
      601 ggattgaaag aatatgggat cacaccagtg acagggaaga gtgattatag ttttattggt
      661 ggacagatgg ttacaagaga attacttgag atggaatcag atttagatgc gatcatctgt
      721 gcaacagacc ggcttgcgtt tggtgcatat aagattttac acgaacataa gaagaggatt
      781 ccagaagaag tgtctgtggc agcgtttgga ggatatgatg aaagtacact cctgacgcca
      841 gagcttacaa cgctaaagtt tgattcctat ggaatgggat accttggagc ggaaacgatt
      901 cttaagatga ttacaggaga accagtatcc aagaaacaga ttgtgggata tgaatttatt
      961 gaaggtggaa gtgtcaggta ataaatttat attggtattt taacaaaaat cgattataat
     1021 aaacctaaca aagaagtcgg tacgatggat aaagcccatc cgtcccggcg gatatagttg
     1081 ctaaatagcc attctattct tgccagggag cagaatggct atttttttta attcagaatg
     1141 gcaatgatta ataacgttac attgataata atcataaatt cttcatatgt actcataata
     1201 tcgccctgcc actcaaaatg tgttgaaata tactcaaaca catcgagaaa ttgaagtcct
     1261 tgcggatact tcttactgct tcaatgtgta tgctttggcg attataaaca atacgctact
     1321 cacaagttct tgtacactcc acagtcgtaa attcctgcga tagcccgcag tacatactta
     1381 cgtttgcttt cattatgcaa cttcgtaagt taaagcatct cttagattaa gagcagcatt
     1441 aaaatcccta ttctcgacat agccacaatc acaacggtat attctatctg aaagcttcaa
     1501 atctttcttg atagcaccac aacagtgaca cattttggat gatggatacc atctgtctac
     1561 gactcttaat tcaataccat tttcatcgca cttagctttc agcttagttc taaattcata
     1621 gaacttttgt gatgcaacgg cttttgatag atgtctgttc ttcatcattc ctgatacatt
     1681 caaatcttca atagttatat aagatggctt ggctttcact atctcggcta ttgatttatt
     1741 gatatagtca gtacggatat tatctatttt atgataaagt ctttgcactt tgagcttttg
     1801 cttttgtata ttcttttgag tagactctcc tttctttaaa ttctcatact tgcgtgagag
     1861 acatctttgt tctctgcgca atttcttttc caatttttta actcttgctg acttattgat
     1921 atttttataa gttttgccat tagaaacaat cgccaagtct ttcaaaccca ggtcaattcc
     1981 tataccgtca ttgctattat tagcaatctt agcgtcggga atttccacaa gaactgacac
     2041 ataatatttg cctgctttga tggatactgt accgcttttg attttccatc cgtctttagt
     2101 tgttggtata tagccttttt ctttcatgcg tacccaacct aaactgggta tgttcaacct
     2161 atgtctctcg catctacaat ctttaggatt attctttacg aaatacattt ttacatcaga
     2221 tttacctttc tttttgaaat taggaaaagc actttgatgt ttaaaaaatc ttgtaaatgc
     2281 agtacatcca tcttcaatag acttttttac agcttttgaa tacgcttctt tcatccatac
     2341 tttttcagga ttattaggaa tgtactcatt attgagccat acgctaaaac tcttgccagt
     2401 cataaacttt tcgcctttat cgtataaagc tttgttgtgc ccgagataga agttgtaaac
     2461 atatctacaa gtgccaatcg tcttgttgat cttgattttt tgctcgactg tcggatttat
     2521 ttccgttttg aagctcttta gcaattttct catccccttc tatttgtttt ttatacttac
     2581 gaaggccgta caatctacaa gaaaaaacgt gaagtataga tataatatcc tgtacaattt
     2641 cttcttgtgg tgatagttct tcattattta ctaccattat ggttgtatta aatttcatac
     2701 agaatttttc aaaccaatca tagccaaatc tgacaaatct atctttatgt gtaactataa
     2761 tagttttgat tttttgttcc attacttcat ctaataattg attccacttt ttacgattgt
     2821 agttaagccc acttccataa tcttcaatac attgatctac aatgatacct ttagcattgc
     2881 aaaactgacg taaaaaagtt acttggtttt gtaaatcatc tttttggttt cttgtagata
     2941 ctctggcata aataacaatc tgacgattat cgttttcagt atttatgccc ttaaactaaa
     3001 gatattggtc ataagtataa taacgcctat tagtcggggt ccgatttgct ttcagaatcc
     3061 cctctctatc ccaacgttgt aacgttttga ctgaaacacc caataattca gcaaaatctt
     3121 ttggtttgta attagtgata ttagatgtgt tcataacaat atcctggtat attttgatat
     3181 atttaatcac tattgacaat gattttgatt acttaaagtt ttctccttcc gcattgctcg
     3241 gaacggaggg ctttgccgtt taccagcctc ttcgttaagt ttactatata atatactata
     3301 atcatacaaa attcaagcaa aagtaataaa ataaaaaata tggtatcgat tccataaaaa
     3361 ctattgacaa acgcataaga aagtaataat atacagatat ggaaccgata ccataataag
     3421 tttcacaata aaaaaaggag agaagatatg gattacgcaa aaatcgccag ccaggtcatc
     3481 gagtatgttg gtggcaaaca aaacattaag tcagttcagc attgtgcaac aagacttcgt
     3541 ttacagttaa aagacaacga tcttcgcaat gaagatgcag tcagcgacat tgaaggagtt
     3601 aagggagtgt tcttaacaca gtcacagttc cagatcattt tcggatcagg acttgtaaac
     3661 ttagtgtgcg acgaagtaca gaagcagtta ggaatctcag tcgatacacc agcagatgat
     3721 gagaaagaag agaagaaagg aaatgttctt cagagattag taaagttatt aagtgatatt
     3781 ttcgtaccga tcattccagc catcgtagca ggtggtctgt taatgggagt gaacaatatc
     3841 ctgacagcag cgatgtttag tggcaagtcc gtcattgatc tgtacccaca gtttaagggt
     3901 ctggcaacag cgatcaacat gtttgcaagt gctccatttg catttttgcc agtattgatc
     3961 ggatttagcg caacgaagaa gtttggcggg aatccatatc ttggagcagc catgggtatg
     4021 atcatggttc atccagatct gttaagtgca tacagcatcg gtatcgccaa agtgccagta
     4081 tgggatatct ttggatttaa gatccaggcg atcggatatc agggaactgt acttccagta
     4141 ttagcagttg catttatttt agcaacgatc gagaagaagt tacataaagt aacaccaaca
     4201 tggttagata acctgacaac accattgatc tctatcatgg taacatcatt tttaacattc
     4261 atctgcgtag gaccagtgtt aagagaagca ggtaacttat tagcagatgg aatcacatgg
     4321 gtatataaca cacttggatt tgtcggtggc ggattattcg gacttgcata tgcaccgatt
     4381 tgtctaacag gaatgcatca cagctttatc gcgatcgaga cacagctgat cgcagccaaa
     4441 gcaacaacag gtggaagttt tatcttcaca acagcaagta tgaacaacgt agcacaggga
     4501 gcagccgtat tagcagtttt attcttaaca aagaatgaga agatgaaatc aatctgttct
     4561 gcatcaggtg tttctgcatt acttggaatc acagaaccag caatgttcgg tgtaacattg
     4621 aaattaaaat atccattcta cgctgccatc atcggttcag ccgtaggtaa tgcatattgt
     4681 gcagcaacag gagttttggc acaggcatta ggagcagcag gacttccggg attcctgtcc
     4741 atgcttccaa aagattactt aaacttcgtg atcggactgc tcttatccat gggagtatcc
     4801 gcagttttaa ctgcagtctt ctggaagaag tttaacgtcg aaggagaaga tcacaaagca
     4861 gagactccgg ttaaagaaga agtaaaagaa atcgaagtac aggaaccagc agatacaaca
     4921 gaagaaacaa atgaattata tgcaccaatg aaaggtgaag ttttagatgt agcaaagtcc
     4981 gcagacccag catttgcaag taaggcaatg ggagaaggtg tagcgatcaa cccaagcgaa
     5041 ggcatcgtat atgcaccagc cgatggaacg atcagtctga tcttccctac aaaacatgca
     5101 atggggatca cattaaactc aggagtagaa cttctgatcc atgcaggaat cgatacagtt
     5161 aagatgaatg gcgaaggttt tgagactttc gtagaaacag gagccaaagt aaagaaaggt
     5221 gataaattat tatcttttga catggatctt gtgaaagaaa aaggatacaa gacacagacg
     5281 atgttcttag ttgcagatgc gaaaggaaaa gaagtcgaag tggttgaagc agcggatgca
     5341 gataataata cgaagatcat gaatttaaaa taatcattat tcgaagaagg aaaggaaacc
     5401 agggcagatg tttgaaaaat acaagaaagt ctataaggca gattatgaaa aatggtataa
     5461 agaaaatgaa gcttacagag aacgtgtggc agcagatcaa gaccgattaa aattccattt
     5521 aatgccaaag acaggatggt taaatgatcc aaatggttta tgccagttta atgggacata
     5581 ccatatttac taccagtaca ctccatttga accaactggt gagttaaaga catggggaca
     5641 ttatacgaca aaagatttta ttcattatga agattttgga ccagtcgtat tcccagatga
     5701 agacattgat gcccatggtg tctattccgg ctctgcattt attgaagatg atacgatcca
     5761 ctatttctac acaggaaatt taaaatattt tgacagagat gattatgact atatcaacag
     5821 tggacgtgga agtaatacga tcacatttac aagtaaagac ggctatgagt ttaccaaaaa
     5881 ggaattgtta atgacaaccg acgattatcc ggctgacatg agcaaccatg tgcgtgatcc
     5941 aaaaatcttt aagaaaaatg agaaatatta catggttctt ggagcaaggg atgtcgaagg
     6001 tgtgggtatg attcttctat atgaatctac agatcttaag aactggacat acaagaaccg
     6061 catcacaact ccacagaagt ttggttatat gtgggaatgt ccggatctgt ttgagatgga
     6121 cggacagtta tacatcatct gctgcccaca gggagtagaa acacaaggca ttgattacga
     6181 aaatgttcat caggtcacag caatgaaatt agactatgat tttgataccg atgaatatga
     6241 aatcacagat atcaaacttt ttgaccgcgg atttgatttc tatgcaccac aaagttttga
     6301 ggatgaaagt ggaagaagaa ttctgatcgg atggatggga atcccagatg cagactatac
     6361 gaatccaacg gaagaagcag gatggcagca tgcactgacg attccaagag aattatccgt
     6421 aagagacggg aaactgattc aggagccgat cgaagaatta aaacagcttc gaagtgaaga
     6481 caaagcagtt tgtacatttt gttatggaca gacgatcatc atgcaaaata acatctatga
     6541 agctgttgta gatttcaaac gatgcagaga aatggtaatg acactgcgcg aagggatcac
     6601 tctttcctat aaagacaata tcctgacctt aaatcttggt gtctatggaa gtggaagatc
     6661 aacaagaaaa gtaagattag aaaaattaga gcatcttcag atttttgcgg atacttcatc
     6721 cttagagatc tttgtaaacc acggagaaga agtttttaca acaagaatct acaatcatca
     6781 tggaagacta ctgattaatg gagaatgttc tggtataatg acagtatatc cattaaaatc
     6841 attcgaaatt gaaggaggaa gacatgaaga ataaattatg tgggatcgga gaagcactga
     6901 tcgattttat tcctgaagtt aagggacaga gactaaaaga tgtaccttca tttaaaagag
     6961 tcgcaggagg agcacctgcc aatgtagtcg gagcagtcac aaagcttggg attccatcca
     7021 aatttttaac aaaacttgga gatgatccat ttggagatta tatcgtggaa gtgttagatg
     7081 aagcaggaat cgacacttct aatattgcaa gagacaaaga aggggaaaca gctttagcat
     7141 tcgtatccct tgcctctgac ggaaaccgtg atttcaaatt ttatagaaaa aatagtgcag
     7201 atttacgcta cagtgtagaa gatatcccgg cagatatctt aaacgattgt ggaatgattc
     7261 atttttgcag tgtagacctt gtggaatctc caatgaagga agctcacaag aaactgatcg
     7321 atatggcgat cgcgcagaat gtgaaagtat cttttgatcc aaacttaaga ttttcattat
     7381 gggacgatct tgatcaatta aaagagacag tcaatgattt cttaaaatac gcagatatca
     7441 tcaagatttc tgacgaagaa ctggaattta tcacaggaca tacagatatc aaagatgctc
     7501 tggacggttt atttgctgat cgtgcaaaat acgttatcta cacaaaagga gctgacggag
     7561 cagaagtgta tacgagaaat ggaatggtag aagcttctgg atataagatc gatgtccgag
     7621 atacgacagg tgctggagat tcgtttatcg gagcattcct atactgtctg ttaaatgatg
     7681 aagtagaaga tttggagtcc gtaagtgatg acacattaag aaactacctt gattttgcca
     7741 atgcttatgc agcgaacaca acaacaaaag aaggtgcatt agcagccatg gcagatcgag
     7801 ctgagatgga tgaatggatg aagacattaa agaaataa
//
