LOCUS       NC_015977              11841 bp    DNA     linear   CON 03-MAY-2020
DEFINITION  Roseburia hominis A2-183, complete sequence.
ACCESSION   NC_015977
VERSION     NC_015977.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604129
            Assembly: GCF_000225345.1
KEYWORDS    RefSeq.
SOURCE      Roseburia hominis A2-183
  ORGANISM  Roseburia hominis A2-183
            Bacteria; Firmicutes; Clostridia; Clostridiales; Lachnospiraceae;
            Roseburia.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Travis,A.J., Kelly,D., Flint,H.J. and Aminov,R.I.
  TITLE     Complete Genome Sequence of the Human Gut Symbiont Roseburia hominis
  JOURNAL   Genome Announc 3 (6), e01286-15 (2015)
   PUBMED   26543119
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11841)
  AUTHORS   Mulder,I.E., Aminov,R.I., Travis,A.J., Lan,A., Gaboriau-Routhiau,V.,
            Garden,K., Logan,E., Delday,M., Coutts,A.G.P., Grant,G.,
            Patterson,A.M., Cerf-Bensussan,N. and Kelly,D.
  TITLE     Bi-directional cross-talk Between a Clostridium symbiont, Roseburia
            hominis, the gut immune system and appetite regulation
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11841)
  AUTHORS   Mulder,I.E., Aminov,R.I., Travis,A.J., Lan,A., Gaboriau-Routhiau,V.,
            Garden,K., Logan,E., Delday,M., Coutts,A.G.P., Grant,G.,
            Patterson,A.M., Cerf-Bensussan,N. and Kelly,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2011) Rowett Institute of Nutrition and Health,
            University of Aberdeen, Greenburn Road, Bucksburn, Aberdeen,
            Aberdeenshire AB21 9SB, Scotland (UK)
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 05/03/2020 17:06:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,349
            CDSs (total)                      :: 3,275
            Genes (coding)                    :: 3,169
            CDSs (with protein)               :: 3,169
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 35 of 106
            Pseudo Genes (incomplete)         :: 65 of 106
            Pseudo Genes (internal stop)      :: 22 of 106
            Pseudo Genes (multiple problems)  :: 14 of 106
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            CP003040.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Roseburia hominis A2-183"
                     /mol_type="genomic DNA"
                     /strain="A2-183"
                     /culture_collection="DSM:16839"
                     /culture_collection="NCIMB:NCIMB 14029"
                     /type_material="type strain of Roseburia hominis"
                     /db_xref="taxon:585394"
     gene            complement(1..867)
                     /locus_tag="RHOM_RS04015"
                     /old_locus_tag="RHOM_04090"
     CDS             complement(1..867)
                     /locus_tag="RHOM_RS04015"
                     /old_locus_tag="RHOM_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0036286,DBD-SUPERFAMILY|0035607"
                     /protein_id="WP_014078985.1"
                     /translation="MGNERFDISIPKEYLTRDKARLLYVSSATYGGDWISTPHTHYCSE
                     LFYVIDGVGQFQVEDNIFPVSANDLVVINPNVSHTELGFNANPLKYIVLGIEGLELSVE
                     NNNANFCIVNFREIKDTVNFYLKVMLREIETRSAGYETVCQDLMEILIVLFGRQTNFST
                     ILSPANKRASHLCDSVRRYIDQNCGDELSLEHLAKFSHVNKYYLVHAFTDEYGISPINY
                     LMHARVKKAEKLLATTDYSLSVISHTCGFSSSSYFSQTFKKVAGVSPSSYRKTVKSDHG
                     EMEDLSR"
     gene            1297..2661
                     /locus_tag="RHOM_RS04020"
                     /old_locus_tag="RHOM_04095"
     CDS             1297..2661
                     /locus_tag="RHOM_RS04020"
                     /old_locus_tag="RHOM_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P29822|3.A.1.1.4"
                     /protein_id="WP_014078986.1"
                     /translation="MRKKLVGTLLIAAMTASTLAGCGSSNSTAGTSASGGAAETSPAES
                     ATEAEGTDAADGEEVLTVWCWDPTFNVYAMKQAEAIYQKDHPNFKLDIQEKVYTDIEQN
                     IITAAEADNYDTLPDIFLMQDNSFQKNIANYPEVFTELSDSGINFDDFSGGKLGDSTAE
                     GKHYGIPFDNGASIMAIRSDMVEAAGLTVEDFKDTTWSEFMELAKKVVDANGVPMLTSS
                     GGSEIVIEMLQSAGASPIVNGEVHLADNAALKKAIEVYKQLIDEGIMVDYTDWDQYIAS
                     MNKGEAAGVIQGCWIMSSIQSAEDQSGKWAIVNMPALDGIDGATNYANCGGASWAVSSN
                     CKNTELAFDFLKTTFGGSVELYDDLLPNAGAISSYLPAAQSDVYNQTSDFYGGQAVYKD
                     IVEFAGKVPAINYGAYYNDIRSALTDAITNVVQNGADIDEEIQNAQDAVEFNISE"
     gene            2826..3704
                     /locus_tag="RHOM_RS04025"
                     /old_locus_tag="RHOM_04100"
     CDS             2826..3704
                     /locus_tag="RHOM_RS04025"
                     /old_locus_tag="RHOM_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P29823|3.A.1.1.4"
                     /protein_id="WP_014078987.1"
                     /translation="MKQKKAKMSLEKKHNLTGWAFLIPAALLIFIFCFYPMIQAIILSF
                     TKKGSGFAGLANYQRILKDKTFQQCLFNTVFYFIIQVPVMLLLALGLAQLLNSPKIKGK
                     SIFRTLIFLPCATSLVSYSMIFKSLFAQDGLVNNIITTLGFDRVDWFQNAWAARFVIII
                     ALIWRWTGYNMVFYLAGLQNIDDSVYEASYIDGATPFQQFMKITIPLLRPTILLTAIMS
                     TSGTLQLFDESVNLTSGGPGKSTMTLAHYVYNISFVETPKFNYAAAISVFILVSVAVLS
                     AIQMKVGDKRD"
     gene            3775..4503
                     /locus_tag="RHOM_RS04030"
                     /old_locus_tag="RHOM_04105"
     CDS             3775..4503
                     /locus_tag="RHOM_RS04030"
                     /old_locus_tag="RHOM_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P29824|3.A.1.1.4"
                     /protein_id="WP_081468031.1"
                     /translation="MICGATNSSIDIVRGKLFPGVYLMENYQSLIANQNLGRAMFNSFR
                     NAIVITVIALLICSIAGYGFEIYHDKGKDLLMNILLLAMMLPFVAIMIPLFKMFSSWKL
                     VNTWIALALPSLSTPFLIMMFRQAARSFPHDIIQAARLEGLSEIRIFFTMFLPIMKSTY
                     GAAMTVTFMNAWNNYLWPTIILQDSNQITMPMLVANLKSGYSVDYGMLMLGVLICTLPT
                     AIIFLCLQKSFANGIAGAVK"
     gene            4500..4940
                     /locus_tag="RHOM_RS04035"
                     /old_locus_tag="RHOM_04110"
     CDS             4500..4940
                     /locus_tag="RHOM_RS04035"
                     /old_locus_tag="RHOM_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044024834.1"
                     /translation="MMNRKYCLDKKEWEEAQAALLLAKQFGLIEDAGIEALEKRRAKKN
                     KENSLYGVRFYSPAMYLQYELTRFKLDFVQPSEQIKKLGVCPGFTEEEKRAFYENNQDL
                     FGRYHGDLFDYEDVRQIIEKRLREDAYDRLIQDILCQSENRA"
     gene            4891..6192
                     /locus_tag="RHOM_RS04040"
                     /old_locus_tag="RHOM_04115"
     CDS             4891..6192
                     /locus_tag="RHOM_RS04040"
                     /old_locus_tag="RHOM_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH136|3.2.1.140"
                     /protein_id="WP_014078990.1"
                     /translation="MTGSYRTYYVNQKTGRDSNDGLTMDSPFASLFQVNRLRLYPGDKV
                     LLARDSVFTDQFLQIKDSGTREHPIEIGSYSPEEEEEGKKPLIAAGGQGIWYQDYGTTL
                     DSPAHVYQGYVSSAVLLYDVEHVVIHDIEISNQAVEILGEDYSAPHKMNRTGVAVVAKD
                     KGVRSGITLRNLSIHDVNGNVYDKHMNNGGIYMTALKPADEKATGVARFKDITVEGCFV
                     YKTSRWGIAVGYTYAHDKFQGAELPEDTFLKYGHENMVIRDNYVKAAGGDGITAMYALR
                     PLVEHNTADSVACEINDRIYCRPENRAGKVAAGIWPWKCKDALFRCNEVADTRLNQDGM
                     AYDADSGDGTIYEYNYSRQNEGGCIMFCQSEAIHNSFCHNVSYDDLGGTVSPSENPDAL
                     LAHNTFYVREGVPFVRNKMGGGTYTEEDNTIIPL"
     gene            6238..8400
                     /gene="gnpA"
                     /locus_tag="RHOM_RS04045"
                     /old_locus_tag="RHOM_04120"
     CDS             6238..8400
                     /gene="gnpA"
                     /locus_tag="RHOM_RS04045"
                     /old_locus_tag="RHOM_04120"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="WP_014078991.1"
                     /translation="MTLKEGRVTIPTNLDVVPETIELMKRWGADAIRDCDGTEFPEELV
                     RTGAKIYATYYTTRKDNEWAKANPDEVQQCYVMTSFYTAVDTDLEIPLMKGISQELMMV
                     NTRDDKERWWEVVDRSTGNVVSADHWEYEEEKGCVVIHDAIPFHEYTVSFLAYIIWDPV
                     HMYNAVTNDWKNFEHQITFDVRQPKTHKYSLERLRKYCADHPYVNVIRYTTFFHQFTLM
                     FDELKREKYVDWYGYSASVSPYILEQFEKEAGYRFRPEYIIDQGYYNNQYRVPSREFKD
                     FQAFQRREVAKIAKEMVDITHEYGKEAMMFLGDHWIGTEPFMEEFATIGLDAVVGSVGN
                     GSTLRLISDIEGVKYTEGRFLPYFFPDTFCDGGDPVKEAKENWITARRAILRKPIDRIG
                     YGGYLKLTLDFPEFLDYVENVCNEFRELYENAKGTIPYCVRKVAVLNSWGKIRSWGCHM
                     VHHALYQKQNYSYAGIIEALSGAPFDVRFISFDDILANPEILKDLDVIINVGDGDTAHT
                     GGGIWENPAISAAIREFVYNGGGFIGVGEPSGHQFQGHYLQLADMLGVEKETGFTLNYD
                     KYNWEEHPDHFILADTTKPVDFGEGKKNIFAYEDTEILVQREKEVQMAVHEFGKGRCVY
                     ISGLPYSFENSRILYRSILWSTHGEKDLHQWFSSNFNVEVHAYVKNGRFCVVNNTYAHQ
                     KTVVYRGDGSSFPLEMEANEIKWYNI"
     gene            complement(8424..10022)
                     /locus_tag="RHOM_RS04050"
                     /old_locus_tag="RHOM_04125"
     CDS             complement(8424..10022)
                     /locus_tag="RHOM_RS04050"
                     /old_locus_tag="RHOM_04125"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012301.1,HMM:NF024242.1,HMM:NF037653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607,DBD-SUP
                     ERFAMILY|0035607"
                     /protein_id="WP_014078992.1"
                     /translation="MVQLLIVDDEPLVQIGIRSMLNWESMGIEIAGTATNGRQAYDIIL
                     EKHPEIVITDIKMPIMDGMQLIEKCQSQEHPPLFILLTSYEEFALVKQAISYQVLDYLV
                     KLELTEDVLNAAIQKALGILEKERKQAAIPETGSDLSLLQDNFYIRLLLNLFETEEQFA
                     TQCDLLSVRFTAAGYTVAYLEMQNTRQSASAVASSDITLYRSSYQMIRNLIVKYVPCRI
                     LFLDTTHLAIIFSLTKEQTGSYHQLLTDALTQTGDMLYNYYSIRILASVGSLVENPRDL
                     SASYSDARQIFPDVRQSDSAVIFADEGNPAREEHNVFNIAIFKDELQKAFNEYDPEALS
                     AVFHDIIDLFSNDKRHYSQALSAASNVLHLTLSCLSNGEEQLNQIFGESANGYNCLYEA
                     ETVPQVLSWMTVLCDGLCRLFSEHNKDYKNRTISAVKKYINEHVDEKITLNQLSDVFNI
                     SPNYLSILFSKYNDMGFIDYVNHAKIECAKEMLDEGTLKIYEISDKLGYESAFYFSRVF
                     KKVEGVSPRDYINRT"
     gene            complement(10066..11841)
                     /locus_tag="RHOM_RS04055"
                     /old_locus_tag="RHOM_04130"
     CDS             complement(10066..11841)
                     /locus_tag="RHOM_RS04055"
                     /old_locus_tag="RHOM_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|F4LXP4|8.A.59.2.1"
                     /protein_id="WP_044024607.1"
                     /translation="MKKKFTTIRSRITLTTITLCIFLSIAVSYISYSFYYKSVKQNMIR
                     TAESSLTLLGSSIDSNIQDAYSFVRNCQANSFILNYLSSQGSQSARITASTYLKGSYNS
                     YDGRYYIKRIFIASYKNRDFIQALASTNNQPFHSADDIRDLYFFDSYYSSPSYSYSEGI
                     LTTDGGSQYLPIIRPLYSQYNSDELGFIYIEMSPNVVTIPLKTAGTYQNNDFYLTLEDH
                     SYVYSNYTLTDANPSYEITGDLSSIASFSDTIVKEISNANGKFVSISVPLAAEHCYITQ
                     LITVNTFYGQLASIIFVIVACAVITGIILHTIFNHMINHPVQELQVRMKHISEGNFERD
                     SSIEWQHELGDIGRSINDLAEDVKTLMDQRIRDENEKREYEYKMLQSQINPHFLYNTLN
                     SIKWMATIQNAPGIAEMTTALSRLLKNIAKGTEKAVPLSAELSLLDDYFTIQKYRYGGT
                     ISLEYRIDDEAALSCLIPRFTLQPVVENSIFHGIEPKGTPGTITIHIFRKNDAILQIDI
                     TDDGVGMTEEQARQLLSENKNEKTEFFREIGVSNVHKRLQYEFGEDYGLRVESRLSEFT
                     TVSVLLPFAPQEDIG"
ORIGIN
        1 ttagcgggaa aggtcttcca tttctccatg gtcagatttg accgtcttgc ggtagctgct
       61 gggagaaact cctgctactt ttttaaaggt ctgggaaaaa tagctggagg aggaaaaacc
      121 acaggtatgg ctgatgacag aaagagagta atctgtggtt gccagcagct tttcagcttt
      181 ttttacgcgg gcatgcatga gatagttgat gggagaaatc ccatactcat cagtaaaggc
      241 atgtaccaga taatatttgt ttacatggga aaattttgcc agatgctcca aggagagttc
      301 atccccgcag ttctgatcaa tatagcgccg aacggaatcg cagaggtggg acgctctttt
      361 gtttgcggga ctgagaatcg tagaaaaatt ggtctggcgt ccgaaaagaa cgataaggat
      421 ttccatcaaa tcctgacaga ctgtttcata gccggcagag cgggtttcga tttcacggag
      481 cattactttt aaatagaagt taacggtatc tttgatttcc ctgaaattta caatgcagaa
      541 gttggcgttg ttattttcaa cggaaagttc cagtccttca atgcccaaaa cgatatattt
      601 taatggattg gcattgaaac caagttccgt gtgggacaca ttgggattga tgaccaccag
      661 atcattggca gaaaccggga agatattgtc ttctacctgg aattgtccca caccgtcaat
      721 tacatagaaa agctcagaac agtaatgggt gtggggcgta ctgatccagt caccaccgta
      781 ggttgcagag gaaacatata aaagtcttgc tttatctctt gttaaatatt cttttggaat
      841 ggagatgtcg aaacgttcat ttcccataaa aacctcccca aaaacttatt ttcatgaata
      901 tcatttttgc gttgtttctg cgctgccttc tgcgcatctg aagttacgta ggaacttcta
      961 aatatgaatc cgtcaggatt cattatatca cgaaaagaca gcggtgtatt gtgttaaatt
     1021 gttgctgaat atcaatatct tgctgaacaa tataatataa cataaaagta gtgaatcgtc
     1081 attttgcata ttaaaatcgg tgtaataaaa aatactacgt taaaatgacg gaaaataata
     1141 atgctgaaaa tgaaaaggtt gtcaaaatac tcatattttt agtaaaaaca gcaagatatt
     1201 gatagaatag gacaacatag aaattgaata gtaggatatg gaaagctata atgaaaccat
     1261 caacaaatga tgtattgttt caaaggagga tttaatatga gaaagaaatt agtaggcaca
     1321 ctgcttatag cagcgatgac cgcttccaca cttgcaggct gcggaagcag taacagcaca
     1381 gcaggcactt ctgcatccgg cggtgcagct gaaaccagcc cggcagagtc tgcaacagaa
     1441 gctgaaggca cagatgcagc agatggcgaa gaagttctta ctgtatggtg ctgggatcca
     1501 accttcaatg tatatgcaat gaaacaggct gaggcaattt accagaaaga tcatcccaat
     1561 tttaaactgg acattcagga gaaggtttac accgatattg agcagaacat cattaccgct
     1621 gctgaggccg ataattatga cacactgccg gacattttcc tgatgcagga taattctttc
     1681 cagaaaaata ttgcaaatta tcccgaggtt ttcacagaat tatcagattc cggtattaat
     1741 tttgacgatt tctccggtgg taaattagga gattctaccg cagaaggaaa acactacggt
     1801 attccttttg ataatggagc atctattatg gcaatccgtt ccgatatggt agaggctgcc
     1861 ggtcttaccg ttgaagattt caaggacaca acctggtctg agttcatgga gctggcgaaa
     1921 aaagttgtcg atgcaaacgg tgttcccatg ctgacaagtt ccggcggttc tgaaattgtt
     1981 atcgagatgc tgcagtctgc aggcgcatct cctatcgtta atggtgaagt tcatctggcg
     2041 gacaacgcag ctttgaaaaa agcaatcgag gtttacaagc agttaatcga tgaggggatt
     2101 atggttgact acaccgactg ggatcagtac attgcatcca tgaacaaggg tgaggctgct
     2161 ggtgttatcc agggctgctg gattatgtca tccattcagt cggcagaaga ccagtctggt
     2221 aagtgggcta ttgtaaacat gcctgcttta gacggcattg atggtgccac caattacgca
     2281 aactgtggtg gtgcaagctg ggcagtatct tctaattgca agaacactga actggcattt
     2341 gatttcctaa agacaacctt tggtggcagc gttgaattat acgatgattt actccctaac
     2401 gccggcgcaa tttccagcta tctgcctgca gcacagtcgg atgtttacaa tcagacttct
     2461 gatttctatg gtggacaggc ggtatacaaa gacattgttg agtttgcagg caaagttccc
     2521 gcaattaact atggtgcata ttataatgat attagaagtg cactgaccga tgcaatcact
     2581 aacgtagtac agaatggtgc tgatattgat gaagaaattc agaatgcaca ggatgctgtt
     2641 gaatttaaca ttagtgaata agtaagtgaa aaatgagcca gtaccgggat cagcgtattg
     2701 cgttcccggt attttttcac aggcgaggat gcacaggttt ttacagaaat atatgtcgcc
     2761 caaagcggcg taaaagcggc gcagaatatc gcgaaacgat attcttttta atcaggagaa
     2821 tttatatgaa acagaagaaa gcaaaaatga gtttggaaaa aaaacataac ctgaccggat
     2881 gggcattttt aattccggct gcacttctta tctttatctt ttgcttttat cctatgatac
     2941 aggcaattat cctgtccttt accaaaaaag gatcaggatt tgcagggctg gcaaattacc
     3001 agagaatatt aaaggataag acgttccagc agtgtctgtt taacactgta ttttacttta
     3061 taatccaggt gcctgtcatg ttacttctgg cactggggct ggcacagctt ttgaacagtc
     3121 cgaagattaa aggaaagagt attttccgta ctcttatctt tcttccatgt gcgacatcac
     3181 tggtatccta ttccatgatt ttcaaatcct tatttgccca ggacggtctg gtaaataata
     3241 ttattacgac ccttggcttt gacagagtgg actggttcca gaatgcatgg gcagcccgtt
     3301 ttgtcatcat cattgcactg atctggagat ggaccggata caatatggtt ttctatcttg
     3361 cgggcttaca gaatattgat gattccgtat acgaagcaag ttatattgat ggagcgaccc
     3421 cttttcagca gtttatgaag attaccattc ccttattgag gcctaccatc ctgttgacgg
     3481 ctattatgtc aacttcaggt acgctacagt tgtttgatga gtctgttaac ctgacttccg
     3541 gaggtcctgg taagtccacc atgacactgg cgcattatgt ttataatatc tcctttgtgg
     3601 aaacacccaa atttaactat gcggcagcaa tttccgtatt tatccttgtg agtgtggcag
     3661 ttctttctgc aatccagatg aaagtaggtg acaaacgtga ctaaagtaaa gacaactatc
     3721 gcttatatta ttttaatcat tgccagtttc ctgtcagcgt ttcccctgta ctatatgatt
     3781 tgtggagcaa ccaactccag cattgatatt gtccgtggaa aactgttccc aggtgtttat
     3841 ctgatggaaa attaccagag tctcattgca aaccagaatc tgggacgagc catgtttaac
     3901 tctttccgca atgcaattgt gattactgtg attgcactgt taatctgctc catcgcaggc
     3961 tatggctttg agatttatca tgacaagggc aaagaccttc tgatgaacat tctgcttctt
     4021 gccatgatgc ttccctttgt tgctattatg attcctttgt ttaaaatgtt ttcttcatgg
     4081 aagctggtta atacctggat tgcactggca ctgccttctc tttctacacc atttctcatt
     4141 atgatgttcc ggcaggcagc ccgttctttc cctcatgata ttatccaggc agcccgtctg
     4201 gaaggactta gtgagatcag gatttttttt accatgttcc tgccaattat gaagtctacc
     4261 tacggcgcag ccatgacagt aactttcatg aatgcctgga ataactatct ctggccaacg
     4321 attattttac aggacagcaa ccagattacc atgcccatgt tggttgcaaa cttaaaaagt
     4381 ggttacagcg tagactatgg tatgttaatg ctgggggtat tgatttgtac ccttcctacc
     4441 gcaattatct ttctgtgctt acaaaagagt tttgccaatg gaattgcagg agcagtgaaa
     4501 taatgaatcg gaaatattgt ctggataaaa aagaatggga ggaggcgcag gcagcccttc
     4561 ttcttgcaaa acagttcggg ctcattgaag atgccggtat cgaggctttg gaaaaaaggc
     4621 gggcaaagaa aaataaggaa aattctcttt atggtgtccg tttttattct ccagccatgt
     4681 atcttcagta cgaactcaca cgatttaagc tggatttcgt tcagccctcg gagcagataa
     4741 aaaagctggg tgtctgtccc ggttttacgg aggaagaaaa gcgggcattt tatgaaaata
     4801 accaggattt gttcgggcgt taccatggag atttgtttga ctatgaggat gtccggcaga
     4861 ttatcgaaaa aagactgagg gaggatgcct atgacaggct catacaggac atattatgtc
     4921 aatcagaaaa ccgggcgtga tagtaacgac ggactgacca tggattcccc gtttgccagc
     4981 ctttttcagg taaacagatt gagactgtac cctggcgata aggtgctgct ggcccgggat
     5041 tccgttttta cagatcagtt tctgcagata aaagacagtg gaaccaggga gcatcccatt
     5101 gaaattggct cttattcacc ggaagaagag gaggagggaa aaaagcccct gattgcagcc
     5161 gggggacagg gcatctggta tcaggattac ggtacaacat tggattcacc tgcgcatgtt
     5221 tatcaaggtt atgtatcctc tgccgtactt ttatatgatg tagagcatgt ggtcatccat
     5281 gatattgaga ttagcaatca ggcagtggaa atcctggggg aagattacag tgctcctcat
     5341 aaaatgaacc gcactggcgt ggctgtggtg gcaaaggata agggagtgcg cagcggcatt
     5401 accctgcgca acctttctat ccatgatgtg aatggcaatg tctatgacaa acatatgaac
     5461 aacggcggca tttatatgac agctttaaag cctgccgatg aaaaagctac cggtgtggcg
     5521 cgctttaaag atatcactgt ggaaggctgc tttgtataca agacaagccg ttggggaatc
     5581 gctgttggat acacctatgc acatgacaaa ttccaggggg cggaactgcc cgaggatacc
     5641 tttttgaaat acggacatga aaatatggtc atccgggaca attatgtaaa agccgctggg
     5701 ggcgatggca tcactgcgat gtatgctttg cgcccattag tagaacacaa tacagcagat
     5761 tccgttgcat gcgaaatcaa tgacaggatt tactgccggc cggagaaccg tgccggaaaa
     5821 gtggcagcag gcatctggcc atggaagtgc aaggatgccc tgttccgctg caatgaagtg
     5881 gcagatacaa gattaaatca ggacgggatg gcttacgatg cggattctgg tgatggaacc
     5941 atttacgaat acaattacag cagacaaaac gagggcggat gcattatgtt ctgccagtca
     6001 gaagcaattc acaacagttt ttgccataat gtgagctatg acgatctggg gggcacggta
     6061 agcccgtcgg agaacccgga tgctttgctc gcccataaca ctttttatgt gagggagggt
     6121 gtgccttttg tccgtaacaa gatgggcggt ggaacttata ccgaggagga taatacgatt
     6181 attccgttgt aaaccgctgt tacaacgact taacttttgc aggaggaaaa gagaccaatg
     6241 actttaaaag agggacgtgt aaccattcct accaacctgg atgtggtgcc ggaaaccata
     6301 gaattgatga aacgctgggg tgctgatgcc attcgtgact gcgacggtac agagttccca
     6361 gaggaactgg tcagaaccgg ggcaaaaatt tatgccacct attatactac ccggaaggac
     6421 aacgagtggg caaaggcaaa cccggatgaa gtacagcagt gctatgtgat gacatccttt
     6481 tacactgctg tggatactga cttggagatt ccccttatga aaggtatatc ccaggaactg
     6541 atgatggtca ataccagaga cgacaaagaa cggtggtggg aggttgtcga ccgttctacc
     6601 ggtaatgttg tgtccgcaga tcattgggaa tatgaggagg aaaagggctg cgtggtcatt
     6661 catgatgcca ttcctttcca tgaatacacg gtcagctttc tggcttacat tatctgggat
     6721 ccagtacata tgtacaatgc ggttaccaac gactggaaaa actttgaaca tcagattaca
     6781 tttgatgtga gacagcctaa gacccacaaa tattccctgg agcgtcttcg caaatactgc
     6841 gcggatcatc cctacgtgaa tgtgattcgc tacacaacct ttttccatca gtttaccttg
     6901 atgtttgatg agttaaagag agaaaaatat gtggactggt atggctattc tgcctctgtc
     6961 agcccttata tcttagagca gtttgaaaag gaggccggtt acaggttccg gcctgagtac
     7021 atcattgacc agggttatta caacaaccag taccgtgtgc ctagcaggga gttcaaagat
     7081 ttccaggcat tccagcgcag ggaggttgcc aaaattgcca aagagatggt agacattacc
     7141 catgagtacg gcaaagaggc aatgatgttt cttggggatc actggattgg aaccgagccg
     7201 tttatggagg aatttgccac cattggactt gatgcggtgg taggcagtgt aggaaacggt
     7261 agtacccttc gtctcatctc cgatattgaa ggtgtaaaat acacggaggg aagattttta
     7321 ccgtactttt tccccgatac tttttgtgac ggcggagacc cggtgaagga ggcaaaggag
     7381 aactggatta ccgcaagacg tgccattctg agaaaaccta ttgaccggat cggctatggt
     7441 ggatatctga agctgaccct ggattttccg gaatttctgg attatgtaga gaatgtgtgc
     7501 aacgagttcc gcgagctgta tgagaacgca aagggaacga ttccttattg cgtgaggaaa
     7561 gtagctgtgt tgaacagctg gggtaaaatt agaagctggg gatgccatat ggtacaccat
     7621 gccctatacc agaaacagaa ttacagctat gcgggaatta ttgaagcatt aagtggtgcc
     7681 cctttcgatg tgcgatttat ttcctttgat gatattctgg caaatccgga aattcttaaa
     7741 gatctggatg tgattatcaa tgtgggagat ggggatactg ctcataccgg tggcggcatc
     7801 tgggagaatc ctgccatttc cgcagctatc cgtgagtttg tatataacgg cggaggtttt
     7861 atcggtgtcg gtgagccgtc tggtcatcag tttcaggggc attacctgca gcttgcagat
     7921 atgctcggcg tggaaaaaga aaccggattt actttaaact acgataaata taactgggag
     7981 gagcatcccg atcacttcat tctggcagat accacgaagc cagtggactt tggtgagggt
     8041 aaaaagaaca tcttcgctta cgaggacacg gaaattctgg tacagcggga gaaggaagtg
     8101 cagatggctg tccatgagtt tggaaagggg cgctgtgttt atatcagcgg tctgccttac
     8161 agctttgaga acagccgtat tctgtaccgc agtattctgt ggagtaccca cggagagaag
     8221 gatctgcatc agtggttcag ttccaacttc aatgtggaag tacatgccta tgtgaaaaat
     8281 ggcaggttct gcgtggtcaa caatacatat gcccaccaga aaactgtggt ttatcgtggt
     8341 gatggcagca gttttcctct ggagatggag gcaaacgaga ttaaatggta taacatttaa
     8401 cacgggcaaa agaaagaagt gcctcaagtc cggttgatat agtcgcgcgg cgacacgccc
     8461 tccacctttt tgaatacccg gctgaaatag aacgcgctct cgtaccccag tttgtcggag
     8521 atctcataga tcttgagcgt tccctcatcc agcatttcct tggcacattc aatctttgcg
     8581 tggtttacgt aatctataaa tcccatatcg ttgtacttgg aaaacaggat cgacagataa
     8641 ttcgggctga tgttgaagac atcggagagc tgattcaacg taatcttctc gtcaacgtgt
     8701 tcattgatat attttttgac cgcggagatg gtgcggttct tgtaatcctt gttgtgctcg
     8761 gaaaacaggc ggcacagacc gtcgcacagc acggtcatcc aggagagcac ctgcggcacc
     8821 gtttccgcct cgtagaggca gttgtacccg ttggcggatt caccaaagat ctggtttaac
     8881 tgttcttccc cgttgctcag gcaggagagg gttaagtgca gcacgttgct cgcggcggat
     8941 aaggcctggg agtaatgtct cttatcattg gaaaacaggt caatgatgtc gtggaagact
     9001 gcggagagtg cttccggatc gtattcgttg aacgctttct gcagctcatc cttaaagatg
     9061 gcaatgttga agacgttgtg ctcctcgcgg gcgggattgc cctcgtcggc aaagataacg
     9121 gcagaatcac tctggcggac gtccgggaaa atctgtctgg cgtccgaata ggaggcggac
     9181 agatctctcg gattttcgac gagagagccg accgatgcaa gaatccttat gctgtaataa
     9241 ttgtagagca tgtcgcctgt ctgtgtgagt gcatcggtca gaagctgatg atagctgccc
     9301 gtctgttctt tggtcaggga aaaaataatc gcaaggtgtg tcgtgtcaag gaacagaatc
     9361 cggcatggca catatttgac gatcagattc cggatcatct ggtagctgct gcggtagagt
     9421 gtgatgtccg acgatgcgac agcggacgca ctctgcctgg tattctgcat ttccagatac
     9481 gccaccgtgt agccggcggc agtgaagcgg acggacagaa gatcgcactg tgtcgcaaat
     9541 tgttcctctg tctcgaatag atttagaagc agccggatat aaaaattatc ctgcaataag
     9601 gagagatcgg aaccggtttc cgggatggct gcctgttttc tctctttttc cagaatgccg
     9661 agagccttct ggatggcagc gtttaacaca tcttccgtca gctcgagctt taccagataa
     9721 tccagcacct ggtaggagat cgcctgtttt accagagcaa attcctcata gctggttaag
     9781 agaataaaaa gaggcggatg ctcttgggac tgacatttct cgatcagctg catgccgtcc
     9841 ataatcggca ttttgatgtc tgtaattaca atttccggat gcttttccag aatgatatca
     9901 tatgcctgcc tgccgtttgt ggcagtgcct gcaatttcaa ttcccatgct ctcccagttt
     9961 aacatggagc ggatgccgat ctgaaccagc ggttcgtcat ccacgataag aagctgtacc
    10021 ataactgtaa ccccatttct ttgttgtaaa aatatctgtc tgcatttagc cgatgtcttc
    10081 ctgcggcgca aacggaagca ggacggacac agtggtaaat tcggaaagcc ggctttccac
    10141 acgcaggccg taatcttcgc cgaattcata ctgcagccgc ttgtgcacgt tgctgacgcc
    10201 gatttccctg aaaaattcag tcttttcgtt cttgttctcg gacaaaagct gtcttgcctg
    10261 ttcttccgtc atgccgacgc cgtcgtcggt aatgtcgatc tgaagaatcg cgtcattttt
    10321 ccggaaaatg tgaatagtga tcgttccggg tgttcccttt ggctcaatgc catgaaagat
    10381 agaattttcc acgaccggct gtaaggtgaa gcgcggaatc agacagctta atgccgcctc
    10441 gtcatcgatc ctgtattcta aggagatggt tcctccataa cggtatttct gaatcgtaaa
    10501 ataatcatcc agaagggaga gttctgcaga gagcggaacc gccttctctg ttccctttgc
    10561 gatgtttttt aaaagtctgg agagtgccgt cgtcatctcc gcgatgccag gcgcattctg
    10621 gatcgttgcc atccatttga tggaattgag cgtgttgtag agaaaatgcg gattgatctg
    10681 gctctgcagc attttgtact cgtactcacg cttctcattt tcatcgcgga tccgttgatc
    10741 catcagtgtt ttcacatctt cggcgagatc gttgatgctt ctgccgatgt cgccgagctc
    10801 atgctgccac tcgatcgagg agtcgcgctc gaaatttccc tcggaaatgt gcttcattcg
    10861 gacctgcagt tcctgtaccg gatgattgat catatgatta aaaatcgtgt gaagaatgat
    10921 accggtaatg acggcgcacg ccacgatgac gaaaatgatg gatgcaagct gtccgtaaaa
    10981 cgtattgacc gtgatcagct gcgtgatata gcagtgttcc gcggcgagcg gaaccgagat
    11041 ggagacgaac tttccgttgg catttgaaat ctcctttacg atcgtatcgg agaaagatgc
    11101 aatggaggag agatctcccg taatctcata ggaagggttg gcgtccgtca gggtgtaatt
    11161 gctgtacaca tagctgtgat cttccaacgt caggtaaaag tcgttgttct ggtaggttcc
    11221 ggcggtctta agcggtatgg tgacgacgtt cggcgacatt tcgatgtaga taaaaccgag
    11281 ctcgtcggaa ttatactggc tgtaaagcgg ccggatgatc ggaagatact gtgaaccgcc
    11341 gtcggttgtc aggatacctt cgctataaga ataggaaggg ctgctgtagt agctgtcaaa
    11401 aaaatagaga tcccggatat catctgccga gtggaagggc tggttgttgg tggaagcaag
    11461 agcctggata aaatcccggt tcttgtagga ggcaataaaa atgcgcttga tatagtagcg
    11521 gccatcgtaa ctgttgtagc ttcccttcag ataagtagag gcggtgatac gcgcactctg
    11581 ggaaccctgt gacgacagat aattcaggat aaaagagttc gcctgacagt tgcggacgaa
    11641 agaataagca tcctggatgt tgctgtcaat ggaggagccg agcagtgtca gagaggactc
    11701 cgcggtgcgg atcatatttt gttttacact tttataatag aaagaatagc tgatatagga
    11761 cacagcgata cttaagaaaa tacagagtgt aatcgtagtc agtgtaattc gggatcgaat
    11821 cgtcgtaaat tttttcttca t
//
