LOCUS       CP025268                9369 bp    DNA     linear   BCT 17-DEC-2017
DEFINITION  Escherichia coli str. K-12 substr. MG1655 strain K-12 chromosome,
            complete genome.
ACCESSION   CP025268
VERSION     CP025268.1
DBLINK      BioProject: PRJNA421841
            BioSample: SAMN08158212
KEYWORDS    .
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Wannier,T.M., Kunjapur,A.M., Rice,D.P., McDonald,M.J., Desai,M. and
            Church,G.M.
  TITLE     Adaptive Evolution of Genomically Recoded Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Wannier,T.M., Kunjapur,A.M. and Church,G.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2017) Genetics, Harvard Medical School, 77 Avenue
            Louis Pasteur, Boston, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2017
            Assembly Method                   :: Velvet v. 2017-06-01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP006698
            Genome Coverage                   :: 69.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/11/2017 15:23:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,810
            CDS (total)                       :: 4,687
            Genes (coding)                    :: 4,444
            CDS (coding)                      :: 4,444
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 108 of 243
            Pseudo Genes (incomplete)         :: 107 of 243
            Pseudo Genes (internal stop)      :: 55 of 243
            Pseudo Genes (multiple problems)  :: 25 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /host="Homo sapiens"
                     /db_xref="taxon:511145"
                     /country="USA: Boston"
                     /collection_date="2017-01-15"
                     /note="C321.deltaA"
     gene            complement(1..2448)
                     /locus_tag="CXP41_18685"
     CDS             complement(1..2448)
                     /locus_tag="CXP41_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GT35"
                     /protein_id="AUG18131.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNAT
                     LFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGALEA
                     MGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNIVNG
                     SQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQIIPG
                     YDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTYSGREL
                     RLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRLLIDEHQ
                     FSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYFLKTLQEQ
                     YPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLFADFAKIFP
                     GRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCDFPMVNHAVH
                     QAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVITRYNRIKADP
                     DAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKVVFIPNYSVSLA
                     QLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMLDHVGADNIFIFG
                     NTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRYRDLVDSLINFGDH
                     YQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDRTIKEYADHIWHIDP
                     VRL"
     gene            complement(2467..3900)
                     /locus_tag="CXP41_18690"
     CDS             complement(2467..3900)
                     /locus_tag="CXP41_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GT5"
                     /protein_id="AUG18132.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAFP
                     DIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFAYT
                     DNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTVHNL
                     AYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYAREITEP
                     QFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAENKRQLQ
                     IAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVLQEGFLA
                     AAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTLPLVRRTG
                     GLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRFVQRQAMAM
                     DFSWQVAAKSYRELYYRLK"
     gene            complement(3900..5195)
                     /gene="glgC"
                     /locus_tag="CXP41_18695"
     CDS             complement(3900..5195)
                     /gene="glgC"
                     /locus_tag="CXP41_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="AUG18133.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPAV
                     HFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLLPA
                     QQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKGARC
                     TVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVFDADY
                     LYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRDVGTLE
                     AYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLVSGGCVI
                     SGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPEGMVIGEN
                     AEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5213..7186)
                     /locus_tag="CXP41_18700"
     CDS             complement(5213..7186)
                     /locus_tag="CXP41_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="catalyzes the hydrolysis of the alpha-1,6-glucosidic
                     linkages in partially depolymerized glycogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH13|GH13_11|CBM48|GH77"
                     /protein_id="AUG18134.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQEH
                     RYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDGEF
                     KDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGLTY
                     LHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYN
                     PVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRG
                     IDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFRFDLAAV
                     MGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDA
                     ARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHN
                     EANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQGTPMLLAGDE
                     HGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVENRWWEEGDGN
                     VRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAG
                     EDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7183..9369)
                     /locus_tag="CXP41_18705"
     CDS             complement(7183..9369)
                     /locus_tag="CXP41_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH13|GH13_9|CBM48"
                     /protein_id="AUG18135.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDATD
                     VWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFGPL
                     IQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRR
                     HPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETASLICG
                     LPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYAKWMGF
                     THLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVILDWVPGH
                     FPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALYWIERFGI
                     DALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMAE
                     ESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTE
                     NFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGR
                     EWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKE
                     RSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTDSMHYHGSNAGNG
                     GTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
ORIGIN
        1 ttacaatctc accggatcga tatgccagat atgatcggcg tactctttga tagtacggtc
       61 agaagagaag tagcccatat tggcaatgtt cagcatcgct tttgcggtcc actcttcctg
      121 aagctcgtag agttcatcga ctttatcctg acaatcgaca tagctgcgat aatccgccag
      181 tacctggtag tgatcgccga agttgatcag cgaatcaacc agatcgcgat agcgacccgg
      241 atcttccgga ctgaatacac cgctgccgat ttgcgtcagc acctgatgca gctcctcatc
      301 tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc agttcttcca cttcttccgc
      361 tgtgttacca aaaataaaga tattgtcagc accgacatga tccagcatct cgacattcgc
      421 accgtccaac gtaccgatag tcagcgcacc gttaagcgca aacttcatgt tactggtgcc
      481 ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga tctgccgccg gaatgatcaa
      541 ctgcgccagg ctaacgctgt agttcgggat gaacacgact ttcagcttat cgccaatctg
      601 cggatcgttg ttgatcactt tcgctacgtc attgatcaaa tgaataatgt gcttcgccat
      661 gtaataggcc gaagccgcct taccgccaaa aatattcacg cgcggtaccc acttcgcatc
      721 cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc aacacattca tcaattgacg
      781 tttgtattcg tgaatacgtt tgatttgtac atcgaacaac gcctttggat tcaccaccac
      841 attcagctgc tgggcgatat actctgccag acgctttttg ttctccagct tcgcctgatg
      901 cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc agctcattaa gcaggctaag
      961 gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg gctgaaagcg atgggttcgc
     1021 taccgccagc cagcgacgcg gcgtcacacc gttggtgacg ttggtgaaac gacccgggaa
     1081 gattttcgca aagtcggcaa acaacgattg caccatcaga ttagagtgca gttccgatac
     1141 accgttaact ttgtggctca caacaaccgc cagccaggcc atacgcacac gacgaccgtt
     1201 ggattcatca atgatcgacg cccgtcccag cagatcggta tcgttcggat actgttcctg
     1261 caaggttttc aggaaatagt cgttgatttc aaagatgatc tgcaggtgac gcggcagaat
     1321 tttacccagc atatcaaccg gccaggtttc cagcgcctcg ctcatcagcg tgtggttagt
     1381 gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc cagctaaatt ggtgctcatc
     1441 gatcagcaga cgcatcatct caggaatcga cagtaccgga tgggtatcat tgagatgaat
     1501 cgcgatttta tccgccaggt tatcgtaggt tttatgcaac tgataatggc ggcttaaaat
     1561 gtcctgaatg gtcgaggaaa ccaggaagta ttcctgacgc aggcgcagct cacgcccgga
     1621 gtaggtggag tcatccggat acagtacgcg agatacgttc tcggagtggt ttttatcttc
     1681 cactgccgcg aagtagtcac cctggttgaa tttaccgagg ttaatttcgc tactggcttg
     1741 cgcactccac aaacgcagcg tgttggtcgc gtcggtgtcg taaccaggga ttatctgatc
     1801 gtaagcgact cccagaatct cttcggtttc aatccagcgc gtttttttac cttcctgctg
     1861 aatgcgaccg ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt cccacgggtt
     1921 accgtattcc agccagtagt ctggcgactc tttctggcta ccgttaacga tgttctgctt
     1981 gaacataccg tagtcatagc ggatgccgta accgcgcccc ggcaacccta acgtcgccag
     2041 agaatcaagg aagcaagccg ccagacgtcc caggccaccg ttaccgaggc ctgggtcatt
     2101 ttcttcatca atcagctctt cgagatttaa ccccatcgct tccagtgcgc cctgtacatc
     2161 ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta cggccaatca aaaactccat
     2221 cgacaggtag taaacctgac gagtttcttg cgacaactgg gcacggtttg aacgtaacca
     2281 gcgctccacg agacgatcgc gcacagcaaa taacgttgcg ttcagccatt catgtttatt
     2341 ggcgacgacc gggtcctttc caatcgtaaa catcagcttg taagcgatag agtgcttaag
     2401 agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat aggcgtttcc
     2461 tgaaaattat ttcaagcgat agtaaagctc acggtacgac ttcgccgcga cctgccagct
     2521 aaaatccatt gccatagcct gacgttgcac aaaccgccac agtgaaggac gggaccacag
     2581 tacaaaagca cgtcgaatag cccgtaacag cgaccaggca ttactatctt caaagacaaa
     2641 cccactggcg acgccatctg caaggttctc aagagaacag tcagaaaccg tatcagcaag
     2701 cccaccggtg cgccgcacta acggcagcgt accgtacttc aatccataaa gttgcgttaa
     2761 gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc gcgccgccca taatgcgatg
     2821 cgaaaatgct tcgtgatagc caatctgaac gcccacctga ccggggtatt ccgctgccgc
     2881 cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg agtagcgcca gctgcccgcc
     2941 ctgctccaga agacccggta aggcttccag caccaggtcg agacctttct ggctggtcag
     3001 acggctcacc actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca ttgcgatttg
     3061 taactggcgc ttattttccg ctttatcttc caacgtatcg cgggtgtaac gcgaggctaa
     3121 cagtaagtcc gtctctggac tccagatttt ctcgtccacg ccgttcagta cgccggaaag
     3181 acgcccttca cggtgacgct gttgcaacag accttccata ccgtaggcaa actgcggttc
     3241 ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg tgatcggcat agtacagacc
     3301 ggccttcagg aaagagattt gtccgttgaa ttccagccca tgaatattaa agaatgacca
     3361 tggcaattgg atgtcattca tgtgatgtgc ataaaacatg ccttgatagg ccaggttgtg
     3421 cacagtaaac accgacttcg ccggacgccc gcgcgccgcc agatacgcag gcgcaaggcc
     3481 tgcatgccag tcgtgcgcat gcaccacatc aggacgccag aatgggtcaa gcccgctggc
     3541 catttctgcc ccaacccacc ccagcagcgc aaaacgcaat acgttgtcgg tataggcaaa
     3601 taagttggta tcgtgatacg ggcttcccgg acgatcatag agatgcggcg cgtcaatcag
     3661 gtaaatgcca accccgttgt aatgaccgaa caacagcgtg atatgtccgg cgaaggtatc
     3721 acgacgggat actacctgcg catcggtcac gccacggcga atatcgggaa atgcaggcaa
     3781 cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc caataacatc
     3841 agccagaccg ccggttttaa gcagcgggaa catctctgaa catacatgta aaacctgcat
     3901 tatcgctcct gtttatgccc taacttccgt agcatttcgc gcgttaccag cacgatgcct
     3961 tcttctgaac gatagaaacg acgtgcatct tcctctgcgt tttcaccaat caccatgcct
     4021 tccggaataa cacaagcacg atcgatgacg cagcggcgca gacggcacga gcgacctacc
     4081 catacttccg gtaacaatac ggcggaatca atgttgcaga atgaattcac gcgaacgcgc
     4141 gagaacagaa cggactgcac caccaccgaa ccggagatca cacaaccgcc ggaaaccagt
     4201 gagttaaggg tcatcccgtg gctaccggag cgatcctgca cgaatttcgc tggcggtaat
     4261 gattcattgt aggtgcgaat tggccaattg cgatcgtaca tatccagttc cggcaccaca
     4321 gaggccagat cgaggttcgc tttccagtaa gcttccagcg tacccacatc gcgccagtac
     4381 ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga acgggtgcgc ataggccaga
     4441 ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt cgtggctgga gttctcatcg
     4501 cgatcgtctt cttccagcag ttcatacaga tagtcggcgt caaagacgta gatacccata
     4561 ctcgccagag atttgctcgg atcgttcggc attgacggcg ggttagcagg tttttcaacg
     4621 aattcgataa ttttatcgtt ctcatcaacc gccataacgc caaatgcgga ggcttcttca
     4681 atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg atcgataagc
     4741 atacgcgagt agtcttgctt gtagatatgg tcgcccgcca ggatcaccac gtattccgct
     4801 ttataacggc ggataatgtc gaggttttgg gtgaccgcat ctgcggtgcc gcgataccag
     4861 ttttcccctt tcattctctg ctgtgctggc agcagatcga caaactcgtt catttcttca
     4921 ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag tgtgggactg gtactgggtg
     4981 atcacgccca tacgacggat cccggagttg atgcagttag acagcgcaaa gtcgataatg
     5041 cggaacttac cgccgaagtg tacggccggt tttgctcgct tattggttaa atccttcagg
     5101 cgggtaccac gtcctcccgc cagtatcagg gcaacagatt tcaatggcag ctggcgcgcc
     5161 aacattaagt gatcgttctt ctctaaacta accatgacta actccttttt tatcatctct
     5221 ggaacacaca caatccgtgt gcaggtccct gccagacagc cgtaatcact gggttatcct
     5281 ctccagcgaa tgggggaatg gcgtgccact ccccagcagg taaaacaatc tctgttacct
     5341 caagcgtggc gttaattgcg atcaaaaagc gatccgagag cagaatttgc agctgtttcg
     5401 gcccgttttg ccactcatcc gtgcttaaag gttgagcata tcgatttagc caacggacat
     5461 tgccgtcgcc ttcttcccac cagcgattct ccaccaaagc gggaatgcgc ttgcgcagat
     5521 ggattaacgc ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag tccaaccagg
     5581 ttaattggtt atcctgacag taggcattgt tattgccatg ctggctgtga ccatgttcgt
     5641 caccggccag taacatcggc gtaccctggg agagcaacaa cgttgttaac agggcgtgaa
     5701 tgctgtcgcg ccgccgttca accaggtcaa gagaaccgcc taacccttct ttaccatgat
     5761 tgttactgta attgttgttg gtcccgtcgc gattttcttc tccgtttgct tcattgtgtt
     5821 tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc atgcgcggtg acgagattaa
     5881 tcgcggcact cggcagacga ccattacgtt taaaaacatc gctggaggca gcaaaacgcc
     5941 cggcaaacgc ccccagaggc aaatcataat gtagccagaa acgacgggca gcatcgcgga
     6001 aatgatcgtt ccactcggca aacagcggcg ggaaatttcc cacctgataa ccaccaggag
     6061 cgatatccca cggttcagca attaacttca cctgcgagag caccgggcag ttctggatag
     6121 cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg gcccatgact gccgccagat
     6181 caaagcggaa accatcgacg tggcaggttt ctacccaata acgcaggcag gcgctggcat
     6241 aatccaccac cgccggatga ctcaaattga gcgtgttgcc gcaaccggtc cagttgtgat
     6301 aatcgccgtc ttctcttatc caataatagc tacggttatc gatcccacgc agcgagaata
     6361 acgggccgtc gaggtccagt tccgcactat ggttgagcac gatatcaaga atgacttcga
     6421 tacccgcttt atgcagtgct ttgattgcat cgcgaaactc atccagcgcc gtttctggcg
     6481 agcaggcata cgccggatgc agcgcaaaca tcgccaccgg gttgtaaccc cagtagttac
     6541 ttagccccat gcgttgcaga cgtggttcac tggcaaactg cgccactggc agcagttcca
     6601 gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac cggatgcccg agggctttat
     6661 aagtgccacg gatctcgacc gggatctccg ggtgcaagta cgttaatcct ttgacatggg
     6721 cttcataaat gatggtgctg ccccacggcg tgcgcggcgg ggcatcatct tcccagtcat
     6781 agtgatcaac caccactacg catttcggcg caatggcggc gttgtcgcga tagtcaggtt
     6841 cattatgacc ggcgtgcagc agcgggttat ctttaaactc cccgtcaatt tgccgcgcgc
     6901 aaggatcaat caacaacttc gccgggttaa agcgatgccc ctcggcgggt tgccaggggc
     6961 catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc cggcagataa ccgtgccaaa
     7021 tgtcgccact gtgccctggc aagtcatagc gatgttcctg gccattggcg tcaaagacac
     7081 acagttctac ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc tgaccgtcgt
     7141 aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg tgtcattctg cctcccgaac
     7201 cagccagata gtggccagcg gtggtagcgt caggcttagt gaatgctgac gaccgtggct
     7261 ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca ttactgccgt gatagtgcat
     7321 ggaatcggta ttgaggattt cacgccattt gcccggctgg tttatgccga agcgataatc
     7381 atgacgcggt accggcgtaa agttactggc aacgatgatt tcgttaccct ctttatcgcg
     7441 acgcacaaag atcagcaccg agcgttcttt gtcatccacc accagccatt caaagccgta
     7501 cgggtcaaaa tccagttcat gcattgcttt atggtggcgg taggtgaggt tcagatcgcg
     7561 caccagacgc tggacaccgt ggtgccagtt atcgccgcct tccaacagat gccagtcgag
     7621 gctggcgtca tggttccact cgcggccctg ggcaaattcg ttacccatga acagtagttt
     7681 cttgcccggg aatgcccaca tccagccata gtaggcgcgc aggttcgcga atttctgcca
     7741 tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg tggaccactt catcatgcga
     7801 caacggcagg acgaagtttt cagtgtagtt gtagagaatc ccgaaggtca gtttatcgtg
     7861 atgatactga cgataaaccg ggtcgagctt catgtagtcc agggtgtcat gcatccagcc
     7921 gaggttccac ttgtaccaga agcccagacc gcccatatcc tgcggacgag aaacgccagg
     7981 gaaatcggta gactcctcag ccattgtcac cgcaccggaa acctgctcac caagaatacg
     8041 gttggtatta cgcaagaatt caatcgcttc aagattctcg cgcccgccaa attcgttcgg
     8101 gatccactcc ccctctttac ggctgtagtc gcgataaatc attgacgcca ccgcatcgac
     8161 gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc gcgttaccga cgaggaagtt
     8221 actgacttca cggcgaccat agttgtagat cagcgtgttc cagtcctgat gatagccttc
     8281 acgcggatcg ctgtgttcat acaagttcgt gccatcaaat tcggcaagcg caaagtcatc
     8341 agtcgggaag tggcctggca cccagtcgag aatcacgttc agaccagctg cgtgtgcggc
     8401 atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg cgggttggcg catacaggcc
     8461 ggttggctga taaccccaac tgccatcgaa gggatgctcg ttaatgggca gtagttcgag
     8521 gtgggtaaag cccatccatt tagcataagg caccagttga tcggccagct cgcggtagct
     8581 caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc aggtgaactt cataaataga
     8641 gattggcgca tcaaactgat tcgctttttt gcgctcttca gtctgtacaa ccttttccgg
     8701 cagcccgcaa ataagagacg cggtttccgg gcgcatttgc gcttcaaagg cataagggtc
     8761 ggacttcaga cgcaagttgc cattggcatc aatcatctcg tatttataga gctgaccgtt
     8821 atgcgcccca gggataaaca gttcccagat gccgctctct ttacgcaggc gcatcgggtg
     8881 acggcgaccg tcccagtagt tgaattgccc aaccaccgag acccgacggg cgtttggagc
     8941 ccagacagag aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg cgcctaaggt
     9001 ttcatacggg cgcaggtgag taccttcaga taatagccag gcatccattt cctggattag
     9061 cggaccaaaa cggtaaggat catcaatcag gttttgctga ccatgccaga caacagccaa
     9121 ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg ctaaagaatc cccgtgagtc
     9181 gagacactcc agttttgcga gtttgcgccc ggttttcggt tcaatcaccc acacatcggt
     9241 agcgtcgggt aaaagggcac ggacttccag tcccgcggtg gttttatgca ttcccagtac
     9301 ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc tatcgatacg
     9361 atcggacat
//
