LOCUS       CP002160                6045 bp    DNA     linear   BCT 08-JAN-2014
DEFINITION  Clostridium cellulovorans 743B, complete genome.
ACCESSION   CP002160
VERSION     CP002160.1
DBLINK      BioProject: PRJNA32609
            BioSample: SAMN00002614
KEYWORDS    GSC:MIGS:2.1.
SOURCE      Clostridium cellulovorans 743B
  ORGANISM  Clostridium cellulovorans 743B
            Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D.,
            Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Chang,Y.-J., Jeffries,C., Kyrpides,N.,
            Ivanova,N., Mikhailova,N., Hemme,C.L. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Clostridium cellulovorans 743B
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D.,
            Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Chang,Y.-J., Jeffries,C., Kyrpides,N.,
            Ivanova,N., Mikhailova,N., Hemme,C.L. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2010) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type       :: bacteria_archaea
            project_name :: Clostridium cellulovorans 743B, ATCC 35296
            collection_date          :: Missing
            lat_lon                  :: Missing
            depth                    :: Missing
            alt_elev                 :: Missing
            country                  :: Missing
            environment              :: Soil
            num_replicons            :: 1
            ref_biomaterial          :: ATCC 35296
            biotic_relationship      :: Free living
            trophic_level            :: Chemoorganotroph
            rel_to_oxygen            :: Anaerobe
            isol_growth_condt        :: Missing
            sequencing_meth          :: WGS
            assembly                 :: Newbler v. 2.3 (pre-release)
            finishing_strategy       :: Finished
            Sequencing Depth         :: 30x
            GOLD Stamp ID            :: Gi02570
            Type Strain              :: Yes
            Greengenes ID            :: 16224
            Gene Calling Method      :: Prodigal
            Isolation Site           :: cellulose digester
            Source of Isolate        :: ATCC 35296
            Cell Shape               :: Rod-shaped
            Motility                 :: Nonmotile
            Sporulation              :: Sporulating
            Temperature Range        :: Mesophile
            Gram Staining            :: Gram-
            Diseases                 :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454/Illumina
            ##Genome-Assembly-Data-END##
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4085037
            Source DNA and organism available from Christopher L. Hemme
            (hemmecl@ou.edu)
            Contacts: Christopher L. Hemme (hemmecl@ou.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Clostridium cellulovorans 743B"
                     /mol_type="genomic DNA"
                     /strain="743B"
                     /type_material="type strain of Clostridium cellulovorans"
                     /db_xref="taxon:573061"
     gene            1..2310
                     /locus_tag="Clocel_0589"
     CDS             1..2310
                     /locus_tag="Clocel_0589"
                     /EC_number="3.2.1.51"
                     /inference="protein motif:PRIAM:3.2.1.51"
                     /note="KEGG: cce:Ccel_1020 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="GH95"
                     /protein_id="ADL50360.1"
                     /db_xref="InterPro:IPR016518"
                     /translation="MSIKDLKNSEDLFKLWYDEPAEVWNWDQALPVGNGKLGAMVFGHV
                     HKEQIQLNEESLWSGGYLDRNNPDALAQLPKVRQLLFDGKLKEAERLCAIAMMGTPEHQ
                     RHYETLGDLFIDFYHDSDEVKNYRRELDINKAMVTVQYEIDGVNFKREILSSAVDDAIV
                     IRITADKKEAISFRGFVGRELFMDTRTALNDSTVALRGGCGGPDSINYSIILKGTSEGG
                     NLYTMGGNIVVENADAVTLYLTSKTSYLSNDFDAVAISTAEAVSKRTYESILQDHIAEY
                     QSYFSRMTLQLGNKQEALELSKIPTDERLERVKEGKLDDGLISLYFHFGRYLLISCSRP
                     GTLPANLQGIWNKHHTSPWGCKFTININTEMNYWPAETCNLSDCHTPLFDLIEKMREPG
                     RHTAKVMYDCGGFVAHHNVDLWGDTAPQDHWMPATVWPMGAAWLCLHLWEHYEFTCDLK
                     FLKKAYETLKESAEFFVDYLIEDRNGYLVTCPSVSPENTYRLESGETGSLCIGPSMDSQ
                     IIYALFSSCIEASELLNTDKEFAETLISLRERLPKPSIGKYGQIMEWAEDYDEVEPGHR
                     HISQLFALHPSNQITVKDTPQLAKAARNTLERRLAHGGGHTGWSRAWIINFWARLEEGE
                     KAYENINALLAKSTLINLLDNHPPFQIDGNFGGAAGVAEMLVQSHSNEINIFPAMPKQW
                     SEGEVTGLCARGGFELSIKWTENKAYVTVTSKAGNRCIIRTPRFAHTSEEVSVEKLQDN
                     VIAFDTIKGEDYHLEF"
     gene            2413..3732
                     /locus_tag="Clocel_0590"
     CDS             2413..3732
                     /locus_tag="Clocel_0590"
                     /EC_number="5.3.1.5"
                     /inference="protein motif:TFAM:TIGR02630"
                     /note="TIGRFAM: xylose isomerase; KEGG: cbe:Cbei_2383
                     xylose isomerase; PFAM: Xylose isomerase domain-containing
                     protein TIM barrel"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="ADL50361.1"
                     /db_xref="GO:0009045"
                     /db_xref="InterPro:IPR001998"
                     /db_xref="InterPro:IPR012307"
                     /db_xref="InterPro:IPR013452"
                     /translation="MREYFANVPKIKYEGKDSKNPLAFKYYNPDEVVGGKTMKEHLRFT
                     LSYWHTLTGAGSDPFGVGTMLRPWDCAEDEMELAKMRMEANFELMDKLGIEYFAFHDRD
                     IAPEGKTLADTNEKLDEIVAYCKELMQKHGKKLLWGTANMFGNPRFVHGAATTCNADVF
                     AYAAAQTKKAMDVTKELGGENYVFWGGREGYETLLNTDLGLEQDNLARFFQMAVDYAKK
                     IGFTGQFLIEPKPKEPTKHQYDFDVATVLGFLRKYNLEKYFKMNIEANHATLAQHTFQH
                     EVAVARVNGVLGSLDVNQGDPNLGWDTDQFPTNIYDATMVMYEVLKNGGIAPGGLNFDA
                     KTRRASFEPEDLFLSYIAGMDTMAKGLRVAYSLLDDAVLENNTSERYKTFSEGIGKDIV
                     EGKVDFESLEKYALENSVISNKSGRQEYLESVVNQYIFND"
     gene            3892..4539
                     /locus_tag="Clocel_0591"
     CDS             3892..4539
                     /locus_tag="Clocel_0591"
                     /inference="protein motif:TFAM:TIGR00875"
                     /note="KEGG: cac:CAC1347 putative translaldolase; TIGRFAM:
                     transaldolase; PFAM: Transaldolase"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="ADL50362.1"
                     /db_xref="InterPro:IPR001585"
                     /db_xref="InterPro:IPR004731"
                     /db_xref="InterPro:IPR018225"
                     /translation="MKIFIDTANVDEIIEANDMGVICGVTTNPSLVAKEGKDFNEVIKT
                     IASIVDGPISGEVNSDDAEGMIVEGREIAKIHKNMVVKIPMTKEGLKAVKVLTAEGIKT
                     NVTLVFSAAQALLAANAGATYVSPFVGRLDDIGSTGMELIEKIAYIFERQDIETEIIAA
                     SIRSSIHAIEAAEAGAHIATIPYGVLMNMAKHPLTDSGIERFKKDWADAFNK"
     gene            4555..6045
                     /locus_tag="Clocel_0592"
     CDS             4555..6045
                     /locus_tag="Clocel_0592"
                     /inference="protein motif:TFAM:TIGR01312"
                     /note="KEGG: tex:Teth514_0154 xylulokinase; TIGRFAM:
                     xylulokinase; PFAM: Carbohydrate kinase, FGGY-like"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="ADL50363.1"
                     /db_xref="GO:0004856"
                     /db_xref="InterPro:IPR006000"
                     /db_xref="InterPro:IPR018483"
                     /db_xref="InterPro:IPR018484"
                     /db_xref="InterPro:IPR018485"
                     /translation="MKFLGVDLGTSSVKIIMVDEEGKLLSSVSKEYPVSYPKNGWSEQN
                     PEDWWNGTKEGIRELMEKSKVSGEEVKGIGFSGQMHGLVILDKDNNVLRPAILWNDQRT
                     QEECDYLNNEIGQEKISKYTGNKALTGFTAPKVLWVRKHEKEIFDKIAHILLPKDYIRF
                     KLTGEFASEVSDASGTLFFDVENRQWSKEMMDILGIKEEQLPKMYESWEVTGKVTKEVA
                     EYIGVSSETVVVGGGGDQAAGAIGTGTVDAGILSVALGTSGVVFASSDSYAVDDENTLH
                     SFCHGNGKYHQMGVMLSAASSLKWWVEEINEDSKDFNKLLADAEKSTVGSNGVIFLPYL
                     TGERTPYSDPNAKGVFFGLNITTKREDMTRAVLEGVAFGLRDSLEILKSLNVPIEEVRV
                     SGGGAQSPLWKQILADVFNVKVSMINSKEGPAYGAAILAIVGCGVYPTVDEACKNLIKV
                     TETVHPIAENVAKYEKPYEVYRKLYPALKDSFKAINNI"
ORIGIN
        1 ttgagcatca aggatttaaa aaactcagaa gatttattta agctctggta tgacgaacca
       61 gcagaagtat ggaattggga tcaagcgttg ccagtaggta atggtaaact tggagctatg
      121 gtttttggtc atgtgcataa agagcaaatt caacttaatg aggaaagcct atggtctggt
      181 ggatatttag atagaaataa tccagacgct ttggctcagc ttccaaaggt tagacaactt
      241 ttgtttgatg gaaaactaaa agaagcagaa agactttgtg ccattgccat gatgggtact
      301 ccagaacatc aaagacatta tgaaacctta ggggacttat ttatagattt ttatcatgat
      361 agtgatgagg ttaaaaatta tagaagagaa cttgatataa ataaagctat ggttacagtt
      421 caatatgaaa tagatggagt gaatttcaaa agagagattc tttccagtgc tgtagatgat
      481 gccatagtta ttagaataac tgctgataaa aaagaagcta tatcttttag aggtttcgtg
      541 ggaagagaac ttttcatgga cacaagaaca gcactaaatg acagtactgt agctttaagg
      601 ggtggctgtg gaggtcctga ctctattaac tactctataa ttttaaaggg aacttccgaa
      661 ggtggaaact tatatactat ggggggaaat atagtagtag aaaatgctga cgcagtaact
      721 ctatatttaa cttctaaaac aagctattta tcaaatgatt ttgatgcagt ggctataagt
      781 acagcagagg cagtttcaaa aagaacttat gagtcaattc ttcaagatca tatagctgag
      841 tatcaaagct actttagtag aatgacttta cagcttggaa ataagcaaga agctttagaa
      901 ctttcaaaga tacctacaga cgaaagactt gaaagagtta aagaaggaaa actagatgat
      961 ggacttattt ctttatattt tcactttgga aggtatttgc taatttcttg tagtagaccg
     1021 ggaacacttc cagctaatct tcaaggaata tggaataagc atcatacatc accttggggt
     1081 tgtaagttta ctattaacat aaatacagag atgaattatt ggccagcgga gacttgtaat
     1141 ttatcggatt gccatacacc tctttttgat cttatagaga aaatgagaga acctggaaga
     1201 cacactgcta aggttatgta tgattgcgga ggatttgttg ctcatcataa cgttgattta
     1261 tggggagata cagcaccaca ggatcattgg atgcctgcga cggtatggcc aatgggagca
     1321 gcttggttat gtcttcatct ttgggagcat tatgaattca cttgtgattt aaagttcttg
     1381 aagaaagcat atgaaacttt aaaagaatca gcagaatttt ttgttgatta tttaattgaa
     1441 gacaggaatg gatacttagt aacttgtcct tctgtatcac ctgaaaatac ttataggctt
     1501 gaaagtggag aaacaggttc tctatgtata ggaccttcaa tggatagcca aataatatat
     1561 gctttattct ctagctgtat agaggcttct gaattattga atacagataa agaatttgca
     1621 gaaactctaa tttctcttag agaaaggtta ccaaaacctt caataggcaa gtatggacaa
     1681 ataatggaat gggcagaaga ttatgatgag gtagagccag gtcatcgtca catatcgcaa
     1741 ttatttgcat tacatccatc aaatcaaata actgttaaag atactccaca acttgctaaa
     1801 gctgcaagaa atactcttga aagaagactt gctcatggtg gcggacatac tggttggagc
     1861 agagcttgga taataaattt ctgggcaaga cttgaagagg gtgaaaaggc ttatgaaaat
     1921 atcaatgctc tacttgcaaa atccacttta atcaacctat tagataacca tccaccattc
     1981 caaattgatg gaaactttgg tggggcagca ggtgttgctg aaatgttagt tcaaagccat
     2041 agcaacgaga ttaatatttt tcctgcaatg ccaaaacaat ggagtgaagg tgaagttact
     2101 ggtttatgtg ctaggggtgg tttcgaacta agtataaaat ggactgaaaa taaagcttat
     2161 gtaacagtaa cttcaaaggc aggtaataga tgcataataa gaactcctag atttgcacat
     2221 actagtgaag aagttagtgt tgaaaaactt caagataatg taatagcttt tgatacaata
     2281 aaaggcgaag attatcattt agaattctaa aactttttaa aatactttta aaaaggtatt
     2341 gaaaaagtat tttaaaagta gtaaaattta aatataaaca ctgtaaacag ttacaaaatg
     2401 gaggtattat taatgagaga atattttgca aatgtaccga aaataaaata cgaaggaaag
     2461 gattcaaaga atccacttgc gtttaaatat tataatccag atgaagttgt tggcggaaag
     2521 acaatgaaag aacacttaag attcacatta tcttactggc atacattaac aggtgctggt
     2581 tcagatccat ttggtgttgg aactatgtta agaccatggg attgtgctga agatgaaatg
     2641 gaattagcta agatgagaat ggaagctaac tttgaactta tggataagct tggaatagaa
     2701 tattttgctt ttcatgatag agatatcgca ccagaaggca aaactttagc tgacacaaat
     2761 gaaaaattag atgaaatcgt agcttactgc aaagaattaa tgcaaaagca tggtaaaaaa
     2821 ttattatggg gaacagctaa catgtttggt aacccaagat tcgttcacgg tgcagctact
     2881 acttgcaacg ctgacgtatt tgcttatgca gcagctcaaa ctaaaaaagc tatggatgtt
     2941 acaaaggaat taggcggaga aaactatgta ttctggggtg gaagagaagg ttacgaaact
     3001 ttattaaaca ctgacctagg attagaacaa gataacttag ctagattttt ccaaatggct
     3061 gttgactatg ctaagaaaat tggatttact ggtcaattct taatcgaacc aaaaccaaag
     3121 gaaccaacaa agcaccaata tgattttgac gttgcaactg ttttaggatt ccttagaaaa
     3181 tataacttag aaaaatattt caaaatgaat atagaagcta accacgcaac acttgcacaa
     3241 cacacattcc aacatgaagt tgctgttgct agagtaaatg gagttcttgg aagtcttgat
     3301 gttaaccaag gagatccaaa cttaggttgg gatacagacc aattcccaac aaacatctat
     3361 gatgcaacta tggttatgta cgaagtatta aagaatggtg gaattgcacc aggtggatta
     3421 aacttcgatg ctaaaacaag aagagcttct tttgaaccag aagatttatt cctttcttat
     3481 atagctggta tggatacaat ggctaaaggt ttaagagttg catattcact tcttgacgat
     3541 gctgtattag aaaacaatac ttctgaaaga tacaagactt tcagcgaagg tatcggtaaa
     3601 gatatagttg aaggaaaagt agacttcgaa tcattagaaa agtatgctct tgaaaattca
     3661 gttatatcta acaaatcagg aagacaagaa tacttagaat ctgttgttaa ccaatacatc
     3721 ttcaacgact aattttaaac taacaaatat tattctaaat aaaagaatag ttttcaagaa
     3781 aactgtaaca aaatacattt aagtagctag aattaaatta cacatagtat agaggcagta
     3841 gttcatattg tctctatatt ttataaataa atataaaagg ggtataaaat tatgaaaata
     3901 tttatagata cagctaatgt tgatgaaatt atcgaagcta acgacatggg agttatttgt
     3961 ggtgtaacta caaacccttc attagtagca aaagaaggaa aagactttaa tgaagttata
     4021 aaaactatag catcaatcgt tgatggtcct ataagtggag aagttaacag tgatgatgca
     4081 gaaggtatga ttgttgaagg aagagaaata gctaaaatcc acaaaaacat ggttgttaaa
     4141 attccaatga ctaaagaagg tttaaaagca gttaaagttt taacagctga aggaattaaa
     4201 actaatgtta ctttagtatt tagtgctgct caagcattgc ttgctgcaaa cgcaggagca
     4261 acttatgtaa gtcctttcgt tggtagatta gacgatatcg gaagcactgg tatggagctt
     4321 atagaaaaaa ttgcttacat atttgaaaga caagatatag aaacagaaat tattgcagct
     4381 agcataagaa gttcaattca cgcaattgaa gcagcagaag ctggagcaca cattgctaca
     4441 attccttatg gagtgttaat gaacatggct aaacacccat taacagattc aggtatcgaa
     4501 agatttaaaa aagattgggc agatgctttt aataaataat agggaggatt aactatgaaa
     4561 tttttaggag tagatttagg aacatcctca gtaaaaatca ttatggttga tgaagaagga
     4621 aagcttttaa gttcagtttc aaaagagtat cctgtaagtt atccaaagaa cggttggtct
     4681 gagcaaaatc cagaagactg gtggaatggc actaaagaag gtataaggga acttatggag
     4741 aaatccaagg ttagtggcga agaagttaaa ggcataggct ttagtggtca aatgcacgga
     4801 cttgtaatcc tagataaaga taataatgta ttgagacctg caattctttg gaatgatcaa
     4861 agaactcaag aagagtgtga ttatttaaat aatgaaattg gacaggaaaa gatatcaaag
     4921 tatactggaa ataaagcttt aactggcttc acagctccta aggttttatg ggttagaaaa
     4981 cacgaaaaag aaattttcga taaaattgct catattttat tacctaaaga ctacatcaga
     5041 tttaaattga caggagaatt tgcttctgaa gtttctgatg cttctggtac acttttcttt
     5101 gatgttgaaa atagacaatg gtcaaaggaa atgatggata tcctaggaat aaaggaagaa
     5161 caacttccaa agatgtatga atcttgggaa gttacaggta aggttactaa agaagtagca
     5221 gaatacattg gtgtgtcatc agaaactgtt gtagttggtg gtggcggaga ccaagctgct
     5281 ggtgctattg gtactggtac agttgatgct ggaattcttt cagtagcgct tggaacttca
     5341 ggagtagtat ttgcgtcaag tgatagttat gctgtagatg atgaaaatac tcttcatagt
     5401 ttctgtcatg gaaacggaaa atatcatcag atgggagtta tgttatcagc tgcatcatca
     5461 ttaaaatggt gggttgaaga aataaacgaa gactctaaag attttaataa gttacttgct
     5521 gatgcagaaa aatctacagt tggtagcaat ggagttatat tccttccata tttaactgga
     5581 gaaagaactc catatagtga ccctaatgca aagggtgttt tctttgggct gaatatcaca
     5641 actaaaagag aagatatgac aagagctgta ttagaagggg tagcatttgg acttcgtgat
     5701 tctcttgaaa tattaaagag tcttaatgtt ccaatagaag aagtaagagt aagcggtggt
     5761 ggagctcaaa gtcctctttg gaaacaaatc ttagctgatg tatttaatgt taaggtttca
     5821 atgattaatt caaaagaagg tccagcttat ggagctgcta tattagctat tgttggctgt
     5881 ggtgtttatc ctactgttga tgaagcttgt aagaacttaa ttaaggtaac agagactgtt
     5941 catcctatag ctgaaaatgt agcaaaatat gaaaaaccat atgaagtata tagaaagctt
     6001 tatccagcac ttaaggattc ttttaaagca ataaataata tttaa
//
