LOCUS       KM066603                6947 bp    DNA     linear   BCT 14-APR-2015
DEFINITION  Corynebacterium alkanolyticum LacI type transcription factor (xylH),
            ABC transporter solute binding protein (xylE), ABC transporter
            permease (xylF), ABC transporter permease (xylG), and
            beta-xylosidase (xylD) genes, complete cds.
ACCESSION   KM066603
VERSION     KM066603.1
KEYWORDS    .
SOURCE      Corynebacterium alkanolyticum
  ORGANISM  Corynebacterium alkanolyticum
            Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
            Corynebacterium.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Watanabe,A., Hiraga,K., Suda,M., Yukawa,H. and Inui,M.
  TITLE     Functional characterization of Corynebacterium alkanolyticum
            beta-xylosidase and xyloside ABC transporter in Corynebacterium
            glutamicum
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
   PUBMED   25862223
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Watanabe,A. and Inui,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2014) Molecular Microbiology and Biotechnology
            Group, Research Institute of Innovative Technology for the Earth,
            9-2 Kizugawadai, Kizugawa-shi, Kyoto 619-0292, Japan
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Corynebacterium alkanolyticum"
                     /mol_type="genomic DNA"
                     /strain="Nakao et al."
                     /db_xref="taxon:909925"
     gene            complement(110..1153)
                     /gene="xylH"
     CDS             complement(110..1153)
                     /gene="xylH"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="AJY53614.1"
                     /translation="MTFPPTPEAAPSGRATIHDVARVAGVSVSTVSKVINGRYGVSAKT
                     SAHVLRVVTELGYESSLIAASLRRGRTHVIGVLVAEFEPFALSLLEGISEALQGTGYDL
                     LSYAGSLSGPTEGQHEGWERRSLSRLGGTLIDGAIIVTPTVEAPSASVPVVAIDPHTGP
                     AGATTVDSDNLEGAIAATRHLIELGHRRIAHVRGRRDLESARLREEGYRMALAEAGIAF
                     DPALVVDGGYRSEESTPAAEQLLSLADPPTAVFAANDLSAIRTIEIARAHGISVPDDLS
                     VVGFDDIPAAASHEPPLTTIRQPLREMGAAAVRILLGKLDGGDDASDAHVRMPAQLVIR
                     ASTAPAR"
     gene            1341..2619
                     /gene="xylE"
     CDS             1342..2619
                     /gene="xylE"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9L027|3.A.1.1.21"
                     /protein_id="AJY53615.1"
                     /translation="MRAHRILTIAAVAGAVALTGCAGGSGGGDDGDVTMTLWHNSTTGP
                     GKQYWEDAAAAFEAENEGVTIDVQSIQNEDMDGRLQTAVNSGDMPDLFMARGGGKLADV
                     VEAGAVKDLSDLISDEVRADYGDGVFDAFTIDGATYGVPTAVLPGGIFYSQDLFADAGV
                     EPAGTMDELVDATEKLKETGVAPIALGAMDAWPAAHWYYFFALRECSQEVMSEIATTLD
                     FSDDCWLAAGEDLADFAETQPFNEGYLTTSAQQGAGSSAGLLANHQAAMELMGGWDVGV
                     IASLTPDQKPLADLSWFPFPAVDGGDGDPTAMMGGVDGYSCSVDSPSACEDFLNFIASQ
                     EWQEKYAEAYQTIPASKAAQGAVTDPALQPLMEAYNEAAYVTVWLDTLLGQSVGNALNT
                     AVVELLSGNGSPEGIVEAVEGAAARG"
     gene            2628..3620
                     /gene="xylF"
     CDS             2628..3620
                     /gene="xylF"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q72H67|3.A.1.1.25"
                     /protein_id="AJY53616.1"
                     /translation="MSSSLIEVPPAADAPASAAGRPARARRGAADWRKRGEIALLAGPA
                     VVVFLAFVIFPVLMAAYYGFFSWKGFGPPTNFIGLQNYITILQDPTFRDAVIHNGVIVV
                     ASLVLQGPLAILFALLLNQRIRGRSIIRVLIFVPYVIAEVVVGTGWSLMLQDAGAVNSL
                     LGKLGIEGASWIADPALAMWTLILIITWKYIGFAVILMLAGLQGIPEELYEAAAIDGAS
                     FWQIQLRITLPLLGPTIRIWAFLSIIGSLQLFDLVNIIWGQYVSATAGTSTMATYMYLN
                     GHMAGNYGFGNAVAVVLFVISLVVALVYQRFVLRRDTEGALTGGKERRK"
     gene            3617..4510
                     /gene="xylG"
     CDS             3617..4510
                     /gene="xylG"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|G4FGN6|3.A.1.1.41"
                     /protein_id="AJY53617.1"
                     /translation="MSSTTLLVVPSARRRRRVDSGIARRPGDGSRAITYLCAVVLIAIC
                     MGPVLYIVLGGFRTNSQITADPSGFPAPWVVTNYTEVLASGTFWQAMASSTITALATTA
                     GVVALGLMASYVIARYDFMGKGALYALFAAGLMFPVTVAITPLYLLVRSLGLTNSLLGV
                     ILPQIAFALPTTIIILVPFLRAIPAEIEEAASIDGASRLGFFFRMVLPLSLPGVITTGI
                     LAFIGSWNGYMLPLFILSDPNLFTLPLGVQNFASQYSVDTARVLAFTSLSMLPALIFFS
                     LFERRIVGGLSGAVKG"
     gene            4537..6942
                     /gene="xylD"
     CDS             4537..6942
                     /gene="xylD"
                     /codon_start=1
                     /transl_table=11
                     /product="GH3"
                     /protein_id="AJY53618.1"
                     /translation="MTELPPLPVVSERVSALLERMTLEEKLAQLVGYWLDRGGEVVAPM
                     QGEMAGDDAGATLAEITRDGIGHYTRVYGTRPVDPAQRAAWLWAEQRRLKAETRLGIPA
                     IVHEECLTGLAAWKAATFPTPLAWGASFDPDLVAEMGAAIGDTMRELGIHQGLAPVLDV
                     IADPRWGRVDECIAEDPYLVGTVGTAYVRGLQSAGVHATLKHFLGYSASKAGRNHAPVS
                     AGPRELADVYLPPFEMALRDGGARSVMNSYTDIDGEPVAAHPGLLTTVLRDELGFDGVV
                     VSDYFSVAFLEVMHGVAADRGEAAVQALRAGIDVELPAGDAYLAPLAEQVRSGGIDIRF
                     VDRAVLRVLAQKEELGLLDEDAFSDEPPVEIDVDSPRHRAIARRLAAESLILLRDEGAV
                     PLPAAARRVAVIGPNADRAEALQGCYSFANHVLAHHPDHPLGFEIPTVREALAAALPQA
                     EIVHARGCEVEGDDRSGFAEAVELVRSADVAVVVAGDQAGLFGRGTVGEGNDVESLDLP
                     GPQRALVEAAVATGTPVVLVLLTGRPYAIEWALDAETAPAAVLQAFFPGEGGGLAIADI
                     LTGASAPSGRLPVSLPRSTGAQPYRYLHPRLGGPSDVTSTDSTPLRPFGFGLTGTTFAY
                     ADLEVDAAAPTDGLIRAAVTVTNTGERAGVDVVQLYGRDVVGSIARPVAQLLGFARVAL
                     EPGASARVVFDVPAQRFAFSDRRLVRVVEPGDVELWVAAHVEASSVRADGDGSISSTRA
                     RVERTLPGDATARATVALTGDVHEVTAADPRLVEVRVDALEPAEAPVS"
ORIGIN
        1 ggcgcccgcg tgtctcaggc gtggcgggaa acggcccccg gttcccacga cgagcgagac
       61 atgcaaccgg ggcgccggtg acggccacgc atgcaggggc gggcaggagc tagcgggcgg
      121 gcgcggtgga tgcccggatg acgagctggg cgggcatccg gacatgggcg tcgctcgcgt
      181 catcgccgcc gtcgagcttg ccgagcagga tccgcacggc ggcggcgccc atctcgcgca
      241 gaggctgccg gatggtcgtc agcggcggct catgcgaggc ggcggcgggg atgtcgtcga
      301 agccgacgac cgacaggtcg tcggggacgg agatcccgtg cgcgcgtgcg atctcgatcg
      361 tgcggatggc cgagagatcg ttcgccgcga acacggccgt cggcgggtcg gcgagcgaca
      421 ggagctgctc ggcggcaggt gtcgactcct cggatcgata cccgccgtcg accacgagcg
      481 cggggtcgaa tgcgatgccg gcctccgcga gggccatccg gtaaccctcc tcgcgcaggc
      541 gggcggactc gaggtcgcgg cgaccgcgga cgtgcgcgat ccgcctgtga ccgagctcga
      601 tgagatgacg ggtcgcggcg atcgcccctt cgaggttgtc gctgtcgacg gtcgtcgcgc
      661 ccgcggggcc ggtgtgcggg tcgatcgcga cgaccggcac gctggcggag ggcgcctcga
      721 cggtcggcgt cacgatgatg gcgccgtcga tgagcgtgcc gcccaggcgg gagagggagc
      781 ggcgctccca gccctcatgc tgcccctcgg tcggccccga gaggcttccc gcgtacgaga
      841 gcaggtcgta gcccgtgccc tgcagggcct cggagatccc ctcgagaaga gagagcgcga
      901 agggctcgaa ctccgcgacg agcacgccga tgacgtgcgt gcggccgcgc cggagcgagg
      961 cggcgatgag cgacgactcg tagccgagct ccgtgaccac gcgcagcacg tgcgccgagg
     1021 tcttggccga gacgccgtac cgcccgttga tcaccttcga gacggtcgac accgacacgc
     1081 cggccacacg cgcgacatca tggatggtcg cgcggccgct cggcgcggcc tccggggtcg
     1141 gcgggaacgt cacgcgtcaa cgctactgcc ggacggcccc ggatggcggt cggacacggc
     1201 cgtgtttcaa tcgttatcga aaacgatgtc gataacgatt gacaatttgt cgtgagcgat
     1261 gacattctcg tcttcgcggg gcgaccgtcg atgtccgacg gatgcccggg atgttcccac
     1321 tcgaggacga ggagagacgc gatgagagca cacaggatcc tgacgatcgc agccgtcgcc
     1381 ggcgcggtgg ccctgacggg ctgcgcgggc ggcagcggcg gcggggacga cggggacgtc
     1441 acgatgacgc tctggcacaa ctcgaccacc ggacccggca agcagtactg ggaggacgcg
     1501 gccgccgcgt tcgaggccga gaacgaaggg gtcacgatcg acgtccagtc gatccagaac
     1561 gaggacatgg atggccgcct gcagactgcc gtgaactcgg gcgacatgcc cgacctcttc
     1621 atggcgcgcg gcggcggcaa gctcgcggat gtcgtcgagg ccggagcggt caaggacctc
     1681 tccgacctca tctcggacga ggtccgcgcc gactacggcg atggcgtgtt cgatgccttc
     1741 acgatcgacg gcgcgaccta cggcgtcccc acggccgtgc tgcccggcgg catcttctac
     1801 agccaggacc tcttcgccga cgcgggcgtc gagccggccg gcacgatgga cgagctcgtc
     1861 gacgcgaccg agaagctcaa ggagacgggc gtcgccccga tcgcgctcgg cgcgatggac
     1921 gcctggccgg ccgcgcactg gtactacttc ttcgcgctgc gcgagtgcag ccaggaggtc
     1981 atgtcggaga tcgcgacgac gctcgacttc tcggacgact gctggctcgc cgcgggcgag
     2041 gacctcgccg acttcgccga gacccagccg ttcaacgagg gctacctgac gacctcggcg
     2101 cagcagggcg ccggctcatc ggcgggcctg ctcgccaacc accaggcggc catggagctc
     2161 atgggcggct gggacgtggg cgtcatcgcc tcgctcaccc ccgaccagaa gcccctcgcc
     2221 gacctgagct ggttcccgtt cccggcggtc gacggcggcg acggcgaccc gacggccatg
     2281 atgggcggcg tcgacggata ctcgtgctcg gtcgactcgc cgtcggcctg cgaggacttc
     2341 ctcaacttca tcgcgtcgca ggagtggcag gagaagtacg ccgaggcgta ccagacgatc
     2401 cccgcctcga aggccgctca gggcgcggtc accgacccgg ccctgcagcc gctcatggag
     2461 gcctacaacg aggccgcgta cgtcacggtg tggctcgaca cgctgctcgg ccagagcgtc
     2521 ggcaacgcgc tcaacacggc ggtcgtcgag ctgctctccg gcaacggctc gcccgagggc
     2581 atcgtcgagg ccgtcgaggg cgcggcggcg cgcggctgag gaacgtcatg agctcctccc
     2641 tcatcgaggt gcccccggcg gccgacgctc ccgcgtcggc cgccgggcgg cccgcccgag
     2701 cgcgtcgcgg cgccgcggac tggcgcaagc gcggcgagat cgcgctcctc gccggcccgg
     2761 cggtcgtcgt cttcctcgcg ttcgtcatct tccccgtgct gatggcggcc tactacggct
     2821 tcttcagctg gaaggggttc ggcccgccca cgaacttcat cgggctgcag aactacatca
     2881 cgatcctgca ggacccgacg ttccgcgacg ccgtgatcca caacggcgtc atcgtggtcg
     2941 catcgctcgt gctgcagggg cccctggcca tcctgttcgc cctcctgctc aaccagcgga
     3001 tccgcgggcg gtcgatcatc cgcgtcctca tcttcgtccc gtacgtcatc gccgaggtgg
     3061 tggtcggaac cggatggagc ctcatgctcc aggacgcggg cgcggtcaac agcctgctcg
     3121 ggaagctcgg catcgagggt gcgtcgtgga tcgccgatcc ggcgctcgcg atgtggacgc
     3181 tcatcctcat catcacgtgg aagtacatcg gcttcgcggt catcctcatg ctcgccggcc
     3241 tgcagggcat ccccgaggag ctgtacgagg ccgccgcgat cgacggcgcg tcgttctggc
     3301 agatccagct gcggatcacc ctgccgctgc tcgggccgac catccgcatc tgggcgttcc
     3361 tgtcgatcat cggatcgctg cagctgttcg acctcgtcaa catcatctgg ggccagtacg
     3421 tctccgcgac ggccggcacc tcgaccatgg cgacgtacat gtacctcaac ggccacatgg
     3481 cgggcaacta cggcttcggc aacgcggtcg ccgtcgtgct gttcgtcatc tcgctcgtgg
     3541 tcgcgctcgt ctaccagcgc ttcgtgctgc gacgcgacac ggaaggcgcc ctcacgggcg
     3601 ggaaggagcg gaggaagtga gctcgaccac cctcctcgtc gtgccgtcgg cacgccggcg
     3661 gcgccgcgtc gacagcggca tcgcacgccg ccccggcgac ggcagccgcg cgatcaccta
     3721 cctctgcgcg gtcgtcctca tcgccatctg catggggccg gtcctctaca tcgtgctcgg
     3781 cggcttccgc acgaactcgc agatcaccgc cgacccatcg ggcttccccg ccccgtgggt
     3841 cgtgacgaac tacaccgagg tgctcgcgag cggcaccttc tggcaggcca tggcgagctc
     3901 gacgatcacg gccctcgcca cgaccgcggg tgtggtcgcc ctcgggctca tggcgagcta
     3961 cgtcatcgcg cgctacgact tcatggggaa gggcgcgctg tacgccctct tcgccgcggg
     4021 actcatgttc cccgtcacgg tcgcgatcac gccgctctac ctgctcgtgc gcagcctggg
     4081 cctgacgaac agcctgctcg gcgtcatcct gccgcagatc gccttcgccc tcccgacgac
     4141 gatcatcatc ctcgtgccgt tcctgcgggc catccccgcg gagatcgagg aggcggcctc
     4201 catcgacggg gcgagccgac tcggcttctt cttccggatg gtgctgccgc tgagcctccc
     4261 cggcgtcatc acgacgggca tcctcgcctt catcgggtcg tggaacgggt acatgctgcc
     4321 gctgttcatc ctgagcgacc cgaacctctt cacgctgccc ctcggcgtgc agaacttcgc
     4381 gtcgcagtac tccgtcgaca cggcgcgcgt gctcgcgttc acgtcgctgt cgatgctgcc
     4441 cgcgctcatc ttcttcagcc tcttcgaacg ccgcatcgtc ggcggcctca gcggggcggt
     4501 caagggatga ccgcccccgg atccgcaggg aacgccatga ccgaactgcc ccctctgccc
     4561 gtcgtgtccg agcgcgtgag cgctctcctc gagcgcatga cgctcgagga gaagctcgcc
     4621 cagctcgtcg gctactggct cgaccgcgga ggcgaggtcg tggcgccgat gcagggcgag
     4681 atggccggcg acgatgcggg agcgactctc gccgagatca cgcgcgacgg catcggccac
     4741 tacacgcgcg tgtacggcac gcggcccgtc gacccggccc agcgcgccgc gtggctgtgg
     4801 gcggagcagc ggcggctgaa ggccgagacc cgcctcggga tccccgcgat cgtccacgag
     4861 gagtgcctca ccgggctcgc cgcgtggaag gccgcgacct tcccgacgcc gctcgcgtgg
     4921 ggcgcgtcgt tcgatcccga cctcgtcgcc gagatgggcg ccgcgatcgg cgacacgatg
     4981 cgcgagctcg ggatccacca ggggctcgcg cccgtgctcg acgtcatcgc cgacccgcgc
     5041 tgggggcgcg tcgacgagtg catcgccgag gatccgtacc tcgtcggcac ggtcggcacc
     5101 gcctacgtgc gcggcctgca gagcgcgggc gtgcatgcga cgctcaagca cttcctcggc
     5161 tactcggcgt cgaaggcggg gcgcaatcac gcccccgtct cggcggggcc gcgagagctc
     5221 gccgacgtct acctgccccc gttcgagatg gcgctccgcg acggcggcgc ccgatcggtg
     5281 atgaacagct acaccgacat cgacggcgag cccgtcgccg cgcatcccgg actgctcacg
     5341 accgtcctgc gggacgagct cggcttcgac ggcgtcgtcg tctcggacta cttctcggtc
     5401 gcgttcctcg aggtcatgca cggcgtcgcg gccgaccgcg gcgaagccgc cgtccaggcg
     5461 ctccgggccg gcatcgacgt cgagctgccc gcgggcgacg cctatctcgc gccgctggcc
     5521 gagcaggtgc ggtcgggcgg gatcgacatc cgcttcgtcg accgcgccgt gctgcgggtg
     5581 ctcgcgcaga aggaggagct gggcctcctt gacgaggacg ccttctctga cgagccgccc
     5641 gtcgagatcg acgtggactc gccgcgacac cgcgcgatcg cccggcgact cgccgcggag
     5701 tcgctcatcc tcctccgcga cgagggagcg gtgccgcttc ccgccgccgc gcgccgcgtc
     5761 gccgtgatcg gccccaacgc cgaccgcgcc gaagcgctgc agggctgcta ctcgttcgcg
     5821 aaccacgtcc tcgcgcatca ccccgaccat ccgctcggat tcgagatccc cacggtgcgc
     5881 gaggctctcg ccgcggcgct cccgcaggcg gagatcgtgc acgcgcgcgg ctgcgaggtc
     5941 gagggcgatg accgctcggg cttcgcggag gccgtcgagc tcgtgcgctc ggccgatgtc
     6001 gcggtcgtcg tcgcgggcga tcaggccgga ctgttcgggc gcggcacggt cggcgagggc
     6061 aacgacgtcg agtcgctcga cctgccgggc ccccagcgcg cgctcgtcga ggccgcggtc
     6121 gcgaccggca cgcccgtcgt gctcgtgctc ctcaccggcc gtccgtatgc gatcgagtgg
     6181 gcgctcgacg cggagaccgc acccgccgct gtcctccagg cgttcttccc cggagagggc
     6241 ggcgggctcg cgatcgccga catcctcacg ggggcgtcag ccccgtcggg acgtctgccg
     6301 gtgtcgcttc cgcgctcgac cggcgcgcag ccgtaccgct atctgcaccc gcgtctgggc
     6361 ggcccgtccg acgtgacgtc gaccgactcg acgccgctgc gtccgttcgg cttcggcctg
     6421 accgggacca cgttcgcgta cgccgacctc gaggtcgacg ccgcggcgcc gaccgacggc
     6481 ctgatccgcg cggcggtgac cgtcacgaac acgggcgagc gcgcaggggt cgacgtcgtc
     6541 cagctctacg ggcgcgacgt ggtcgggtcg atcgcgcgcc ccgtggcgca gctgctcggc
     6601 ttcgcgcgcg tcgcgctcga gcccggcgcg tccgcgcgcg tcgtgttcga cgtgcccgct
     6661 cagcgcttcg cgttcagcga ccgccgtctc gtccgcgtcg tcgagcccgg cgacgtcgag
     6721 ctgtgggtcg cggctcacgt cgaggcgtcg tctgtccgcg ccgacggcga cggctcgatc
     6781 agcagcacgc gggcgcgggt cgagcggacg ctcccgggcg acgcgaccgc gcgcgcgacc
     6841 gtcgcgctca cgggcgacgt gcacgaggtg acggccgcag acccccggct cgtcgaggtg
     6901 cgcgtcgacg ccctcgagcc ggccgaggca cccgtcagct gagcgct
//
