LOCUS       TTPULSA                12854 bp    DNA     linear   BCT 16-APR-2002
DEFINITION  Thermoanaerobacterium thermosulfurigenes pullulanase (amyB)
            maltose-binding protein (amyE), membrane proteins for maltose
            transport (amyD and amyC), and alpha-cyclodextrin
            glycosyltransferase (amyA) genes, complete cds.
ACCESSION   M57692
VERSION     M57692.1
KEYWORDS    pullulanase; maltose-binding protein; membrane protein for maltose
            transport; alpha-amylase.
SOURCE      Thermoanaerobacterium thermosulfurigenes
  ORGANISM  Thermoanaerobacterium thermosulfurigenes
            Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
            Thermoanaerobacterales Family III. Incertae Sedis;
            Thermoanaerobacterium.
REFERENCE   1  (bases 10038 to 12854)
  AUTHORS   Bahl,H., Burchhardt,G., Spreinat,A., Haeckel,K., Wienecke,A.,
            Schmidt,B. and Antranikian,G.
  TITLE     alpha-Amylase of Clostridium thermosulfurogenes EM1: nucleotide
            sequence of the gene, processing of the enzyme, and comparison of
            other alpha-amylases
  JOURNAL   Appl. Environ. Microbiol. 57 (5), 1554-1559 (1991)
   PUBMED   1854207
REFERENCE   2  (bases 8553 to 11459)
  AUTHORS   Bahl,H., Burchhardt,G. and Wienecke,A.
  TITLE     Nucleotide sequence of two Clostridium thermosulfurogenes EM1 genes
            homologous to Escherichia coli genes encoding integral membrane
            components of binding protein-dependent transport systems
  JOURNAL   FEMS Microbiol. Lett. 65 (1), 83-87 (1991)
   PUBMED   1874408
REFERENCE   3  (bases 1 to 7234)
  AUTHORS   Matuschek,M., Burchhardt,G., Sahm,K. and Bahl,H.
  TITLE     Pullulanase of Thermoanaerobacterium thermosulfurigenes EM1
            (Clostridium thermosulfurogenes): molecular analysis of the gene,
            composite structure of the enzyme, and a common model for its
            attachment to the cell surface
  JOURNAL   J. Bacteriol. 176 (11), 3295-3302 (1994)
   PUBMED   8195085
REFERENCE   4  (bases 1 to 12854)
  AUTHORS   Sahm,K., Matuschek,M., Muller,H., Mitchell,W.J. and Bahl,H.
  TITLE     Molecular analysis of the amy gene locus of Thermoanaerobacterium
            thermosulfurigenes EM1 encoding starch-degrading enzymes and a
            binding protein-dependent maltose transport system
  JOURNAL   J. Bacteriol. 178 (4), 1039-1046 (1996)
   PUBMED   8576036
REFERENCE   5  (bases 1 to 7234)
  AUTHORS   Matuschek,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-1992) Markus Matuschek, Institut fuer
            Mikrobiologie, Grisebachstrasse 8, 37077 Goettingen. Germany
REFERENCE   6  (bases 7235 to 12854)
  AUTHORS   Matuschek,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-1994) Markus Matuschek, Institut fuer
            Mikrobiologie, Grisebachstrasse 8, 37077 Goettingen. Germany
COMMENT     On or before Apr 5, 2002 this sequence version replaced M57580.1,
            gi:1234894, gi:48767, gi:1004351.
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Thermoanaerobacterium thermosulfurigenes"
                     /mol_type="genomic DNA"
                     /strain="EM1"
                     /db_xref="taxon:33950"
                     /clone="pCT3, pCT4, pCT5, pCT14, pCT25, pCT26"
                     /clone_lib="DSM 3896"
     regulatory      123..128
                     /regulatory_class="ribosome_binding_site"
     CDS             138..1109
                     /note="ORF1"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAB00840.1"
                     /translation="MKNFAYLNGPKVVVIGGGTGLSTMLRGLKKYTHNITAIVTVADDG
                     GGSGVLREDLGMLPPGDIRNCILALRNTEPTMEKLLQYRFTDGMLKGQSFGNLFLAAMN
                     GISISFEEAVKKMSEVLAVSGKVLPVTLDDVKLKAKLKNGIVIDGESLIPKLQMKEKSP
                     IERIFLEPKDAKPVKEALIDIMDADEIILGPGSLYTSIIPNLLVNDVCEAIEDSKAIKV
                     YVCNIMTQPGETIGYDANAHVDALFLHGLKSLDYVIVNNGEIPYEYKDRYKEDMSQPVS
                     YDVESFKQKGIKVIEKDVLAIRNNYIRHDEQKLAEILMGLLE"
     regulatory      1094..1130
                     /regulatory_class="terminator"
                     /note="ORF1"
     gene            1230..7084
                     /gene="amyB"
     regulatory      1230..1235
                     /regulatory_class="minus_35_signal"
                     /gene="amyB"
     regulatory      1253..1258
                     /regulatory_class="minus_10_signal"
                     /gene="amyB"
     mRNA            1266..>10612
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="polycistronic; amyB, amyE, amyD, amyC"
     mRNA            1266..>7084
                     /gene="amyB"
                     /experiment="experimental evidence, no additional details
                     recorded"
     gene            1266..4111
                     /gene="amyB truncated"
     mRNA            1266..>4111
                     /gene="amyB truncated"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      1421..1426
                     /regulatory_class="ribosome_binding_site"
                     /gene="amyB"
     CDS             1434..7019
                     /gene="amyB"
                     /EC_number="3.2.1.41"
                     /function="hydrolysis of alpha-1,6 and alpha-1,4 glycosidic
                     linkages"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="extracellular enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13_39|CBM34|GH13|CBM20"
                     /protein_id="AAB00841.1"
                     /translation="MNKKLFTNRFISFNMSLLLVLTAVFSSIPLHSVHAADNASVVANI
                     VGEFQDQLGDSNWNIDSNITIMQYMGNGLYEFTTPTQLKAGSYQYKVALNHSWDGGGVP
                     SQGNLTLNLANDSYVTFWFDYNTQSVTDSTKYTPIANDKLPRLVGTIQSAIGAGNDWKP
                     ETSTAIMTDDNFDNVYSYTAHVPKGDYQYKVTLGNTWDENYGANGVKDGSNIQINVTND
                     ADITFYYDANTHNIWTNYSPILTGLDNNIYYDDLKHDTHDSFFRNPFGAVKVDQTVTLR
                     IQAKNHDLESARISYWDDINKIRIELPMTRIGESPDGNFEYWEIKLSFDHPTRIWYYFI
                     LKDGTKTAYYGDNDDQLGGVGKATDTVNKDFELTVYDKNFDTPDWMKGAVMYQIFPDRF
                     YNGDTSNDHAKTLSRGNDPIEFHNNWNDLPDNPNNAGTPGYTGDGIWSNDFFGDLKGID
                     DKLDYLKGLGVSVIYLNPIFESPSNHKYDTADYTKIDEMFGTTQDFEKLMSDAHAKGIK
                     IILDGVFNHTSDDSIYFNRYGKYPGLGAYQAWKEGNQSLSPYGDWYTINSDGTYECWWG
                     YDSLPVIKSLNGSEYNVTSWANFIINDENAISKYWLNPDGNLNDGADGWRLDVENEVAH
                     DFWTHFRNAINTVKFEAPMIAENWGDASLDLLGDSFNSVMNYQFRNDIIDFLIGQSFDD
                     GNGQHNPIDAAKLDQRLMSIYERYPLPAFYSTMNLLGSHDTMRILTVFGYNSADPNENS
                     DAAKQLAEQKLKLATILQMGYPGMADIYYGDEAGVSGGKDPDDRRTFPWGNEDTTLQDF
                     FKNISSIRNNNQVLKTGDLETLYAQNDVYAIGRRIINGKDAFGTSYPDSAAIVAINRSK
                     SDKQIAIDTTKFLRDGVTFKDLINNNVSYSISNGQIVIDVPAMSGVMLISDDGQDLTAP
                     QAPSNVVVTSGNGKVDLSWLQSDGATGYNIYRSSVEGGLYEKIASNVTETTFEDANVTN
                     GLKYVYAISAIDELGNESGISNDAVAYPAYPIGWVGNLTQVSDNHIIGVDKPTEDIYAE
                     VWADGLTNSTGQGPNMIAQLGYKYVSGTVYDSVYGSVYNSVYGVDDSGFTWVNAQYVGD
                     IGNNDQYKASFTPDKIGQWEYLMRFSDNQGQDWITTSTLSFYVVPSDDLIKPTAPYLNQ
                     PGTESSRVSLTWNPSTDNVGIYDYEIYRSDGGTFNKIATVSNEVYNYIDTSVINGVTYN
                     YKVVAVDLSFNRTESNVVTIKPDVVPIKVIFNVTVPDYTPDAVNLAGTFPNATWDPSAQ
                     QMTKIDNNTYSITLTLDEGTQIEYKYARGSWDKVEKDEYGNEFASNRKVTIVNQGNNEM
                     TINDTVYRWRDIPIFIYSPSSNMTVDSNISTMEVKGNTYKGAKVTINGDSFVQDKNGVF
                     TKDVSLNYGVNKIKIHVEPNDGSVYGNDQGRITELTKDIEIDVIRQENNSGSGTGNNNT
                     STSGSNSSSTGSGSTGSTSITSNISNTSNTSNTIGVITKNGNVITLTLDAGKAKDLIVN
                     SKDKKVVFDITTIGEGQQKVVQISKDILDTSAANGKDIVIKSDNASIALTKDALNQNQI
                     QNGVNVSIKDNGKPNVTNYVSLSNVVDITISGISGNVTLAKPVEVTLNISKANDPRKVA
                     VYYYNPTTNQWEYVGGKVDASSGTITFNATHFSQYAAFEYDKTFNDIKDNWAKDVIEVL
                     ASRHIVEGMTDTQYEPNKTVTRAEFTAMILRLLNIKEETYSGEFSDVKSGDWYANAIEA
                     AYKAGIIEGDGKNARPNDSITREEMTAIAMRAYEMLTQYKEENIGATTFSDDKSISDWA
                     RNVVANAAKLGIVNGEPNNVFAPKGNATRAEAAAIIYGLLEKSGNI"
     sig_peptide     1434..1538
                     /gene="amyB"
                     /experiment="experimental evidence, no additional details
                     recorded"
     mat_peptide     1539..7016
                     /gene="amyB"
                     /product="unnamed"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      7041..7084
                     /regulatory_class="terminator"
                     /gene="amyB"
     gene            7138..8785
                     /gene="amyE"
     regulatory      7138..7143
                     /regulatory_class="minus_35_signal"
                     /gene="amyE"
     regulatory      7161..7166
                     /regulatory_class="minus_10_signal"
                     /gene="amyE"
     mRNA            7173..>10612
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="polycistronic; amyE, amyD, amyC"
     mRNA            7173..>9697
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="polycistronic; amyE, amyD"
     mRNA            7173..>8785
                     /gene="amyE"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      7389..7394
                     /regulatory_class="ribosome_binding_site"
                     /gene="amyE"
     CDS             7404..8744
                     /gene="amyE"
                     /note="extracellular component of an ABC transporter;
                     lipoprotein (putative)"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8NMV3|3.A.1.1.45"
                     /protein_id="AAB00842.1"
                     /translation="MRKTSKVIALLLVLLMAFSVLITGCGNSTKSDQSKTSENSSTQNK
                     TETTSQPVTLDIFQFKVEIKDALQNAINTYMKENPNVKINLETVGGGQDYGAALKAKFN
                     SGSEPAIYNVGGPQDVATWKPKLADLSDTKAAKAALPGTLTGVTQDGKIYGLPFDLEGY
                     GLIYNKQIFSKAGIDPSSIKTFDALVNAVKTLDSKKKELGIEAVFAFPAKETWVTGLHL
                     SNAFLSPEFDGDVMKAFNSKTVDFKYGDAMKQMIDLQNKYSVQPTASLDYSTQVEKLFG
                     TGKVAMIQQGNWVYPTLESLDKNFAQNDIGMLPYPVPGYKEDSYPEGVPMYWAVNSDKP
                     ADVQKAAKDFLDWLYTSDEGKQIVTQQFKFVPAYTGYNSNSISDPLGMQLFVAASQGKT
                     YGWVFMGYPNNWGQNVLGADIQLYVNGSLSWDKVISHAKQAWADARK"
     sig_peptide     7404..7475
                     /gene="amyE"
     mat_peptide     7476..8741
                     /gene="amyE"
                     /product="unnamed"
     regulatory      8756..8785
                     /regulatory_class="terminator"
                     /gene="amyE"
     gene            8805..9697
                     /gene="amyD"
     regulatory      8805..8810
                     /regulatory_class="ribosome_binding_site"
                     /gene="amyD"
     CDS             8819..9697
                     /gene="amyD"
                     /note="membrane component of an ABC transporter"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8NMV4|3.A.1.1.45"
                     /protein_id="AAB00843.1"
                     /translation="MAKKAKFFKNGIWYWLFIAPTLLSLIIVVLIPFIIGIYYSFTDWN
                     GINQPVFIGLKNFMTLRDDAEFWNSIIFTAKFAVACIVIINVVGLSLAMLVTRKIFARN
                     FMRTAFYLPNLIGGLILGFIWNFIFVDVFQTISDATHIGWLGGWLSTTNTGFWGLVIVT
                     SWQMIGYVMVIYIAYIESIPTDLIEASKIDGANSWQQFRNVVFPLIAPAFTVSLFITLS
                     NSFKLFDQNLSLTAGAPGNTTQMITLNIYQTAFSAQEMAVGQAKAVIMFLIIAVISVIQ
                     VYLTQKREVEM"
     mat_peptide     8819..9694
                     /gene="amyD"
                     /product="unnamed"
     gene            9681..10521
                     /gene="amyC"
     regulatory      9681..9686
                     /regulatory_class="ribosome_binding_site"
                     /gene="amyC"
     CDS             9697..10521
                     /gene="amyC"
                     /note="membrane component of an ABC transporter"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8NMV5|3.A.1.1.45"
                     /protein_id="AAB00844.1"
                     /translation="MRKVHVQKYLLTFLGIVLSLLWISPFYIILVNSFKTKLELFTNTL
                     SLPKSLMLDNYKTAAANLNLSEAFSNTLIITVFSILIIAIFSSMTAYALQRVKRKSSVI
                     IYMIFTVAMLIPFQSVMIPLVAEFGKFHFLTRSGLVFMYLGFGSSLGVFLYYGALKGIP
                     TSLDEAALIDGCSRFRIYWNIILPLLNPTTITLAVLDIMWIWNDYLLPSLVINKVGSRT
                     LPLMIFYFFSQYTKQWNLGMAGLTIAILPVVIFYFLAQRKLVTAIIAGAVKQ"
     mat_peptide     9697..10518
                     /gene="amyC"
                     /product="unnamed"
     regulatory      10569..10612
                     /regulatory_class="terminator"
                     /note="amyE, amyD, amyC"
     gene            10575..>12828
                     /gene="amyA"
     regulatory      10575..10580
                     /regulatory_class="minus_35_signal"
                     /gene="amyA"
     regulatory      10598..10603
                     /regulatory_class="minus_10_signal"
                     /gene="amyA"
     mRNA            10611..>12828
                     /gene="amyA"
                     /experiment="experimental evidence, no additional details
                     recorded"
     regulatory      10683..10688
                     /regulatory_class="ribosome_binding_site"
                     /gene="amyA"
     CDS             10696..12828
                     /gene="amyA"
                     /EC_number="2.4.1.19"
                     /function="hydrolysis of alpha-1,4 glycosidic linkages"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="extracellular enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13_2|CBM20"
                     /protein_id="AAB00845.1"
                     /translation="MKKTFKLILVLMLSLTLVFGLTAPIQAASDTAVSNVVNYSTDVIY
                     QIVTDRFVDGNTSNNPTGDLYDPTHTSLKKYFGGDWQGIINKINDGYLTGMGVTAIWIS
                     QPVENIYAVLPDSTFGGSTSYHGYWARDFKRTNPYFGSFTDFQNLINTAHAHNIKVIID
                     FAPNHTSPASETDPTYAENGRLYDNGTLLGGYTNDTNGYFHHYGGTDFSSYEDGIYRNL
                     FDLADLNQQNSTIDSYLKSAIKVWLDMGIDGIRLDAVKHMPFGWQKNFMDSILSYRPVF
                     TFGEWFLGTNEIDVNNTYFANESGMSLLDFRFSQKVRQVFRDNTDTMYGLDSMIQSTAS
                     DYNFINDMVTFIDNHDMDRFYNGGSTRPVEQALAFTLTSRGVPAIYYGTEQYMTGNGDP
                     YNRAMMTSFNTSTTAYNVIKKLAPLRKSNPAIAYGTTQQRWINNDVYIYERKFGNNVAL
                     VAINRNLSTSYNITGLYTALPAGTYTDVLGGLLNGNSISVASDGSVTPFTLSAGEVAVW
                     QYVSSSNSPLIGHVGPTMTKAGQTITIDGRGFGTTSGQVLFGSTAGTIVSWDDTEVKVK
                     VPSVTPGKYNISLKTSSGATSNTYNNINILTGNQICVRFVVNNASTVYGENVYLTGNVA
                     ELGNWDTSKAIGPMFNQVVYQYPTWYYDVSVPAGTTIQFKFIKKNGNTITWEGGSNHTY
                     TVPSSSTGTVIVNWQQ"
     sig_peptide     10696..10776
                     /gene="amyA"
     mat_peptide     10777..12825
                     /gene="amyA"
                     /product="unnamed"
                     /experiment="experimental evidence, no additional details
                     recorded"
ORIGIN
        1 gatctgtgac tattgcaaat gcactgtacg atctgcttag aaaaagggat tacacagcta
       61 taataaatca tagggatatt agagaagaat aagtgatatt ttcatttata tgacttattg
      121 caaaggggtt gctcgttatg aagaattttg cttacttaaa tggaccaaaa gtcgtggtta
      181 ttggcggtgg tacaggatta tcaacaatgc ttagaggact taagaagtac acccataaca
      241 ttactgcaat tgtaacagtt gcagatgatg gaggaggttc tggtgtatta agagaggatt
      301 taggcatgtt gccgcctggt gatataagaa actgtatact ggcgttacga aatactgaac
      361 ctacaatgga aaagctttta caatacaggt ttactgatgg tatgcttaaa gggcaaagct
      421 ttggaaatct ttttttagct gctatgaatg gcatatcaat tagttttgaa gaagctgtaa
      481 agaaaatgag tgaagtttta gctgtgtcag gaaaggtttt gcctgtcaca ttggatgatg
      541 tcaaactaaa agctaagtta aaaaacggta tagtcataga tggagaatct ttaataccga
      601 aacttcaaat gaaagaaaaa agcccgatag aaagaatatt tttggagccg aaagatgcaa
      661 aaccagtaaa agaagcctta atagatatca tggatgctga tgaaattata ttaggtcctg
      721 ggagtctata caccagcata ataccaaatc tattagtcaa tgatgtgtgt gaagcaatag
      781 aagattcaaa agcaattaag gtatatgtgt gcaatataat gacacagcct ggtgagacaa
      841 ttggctacga tgccaatgca cacgttgatg cgctttttct gcatgggtta aaatctcttg
      901 actatgtcat cgtaaataat ggtgaaattc cttatgagta caaagataga tataaagaag
      961 acatgtcgca acctgtaagt tatgatgtag aaagctttaa gcaaaaggga ataaaagtca
     1021 tcgaaaaaga tgtgttagca ataagaaaca attatataag gcatgatgag caaaagcttg
     1081 cagagatact aatgggacta cttgagtgat ttattcaagg tccttttttt aacaataaaa
     1141 atgataataa atttattctt ataaaaaaag gtatgaatag agtttgaaaa ttattaaata
     1201 cttcacgcat taaaatttga ttgacattat tgacattctt tagtaaagat aatataatat
     1261 tactcaaagg aaaaccggtt gcgcaaattt tttgcacatt catttcgtta cagcaatctc
     1321 aaactatttt gttctaattt cagtggataa acgattttga aaggaggtcg attgtaggac
     1381 aatacggtta atttaaattt gtttcaattc aaaaaataat gggaggcttt gatatgaata
     1441 aaaagctttt taccaacaga ttcataagct ttaatatgtc gcttttatta gtattgacag
     1501 cggtattttc ttcaatacca cttcacagtg tacatgctgc tgataatgca agcgttgtag
     1561 caaatatcgt aggggaattt caagatcaac tgggtgattc taactggaat attgatagta
     1621 acattaccat aatgcagtat atgggaaatg gactgtatga atttactacg ccaactcaac
     1681 taaaagctgg tagctatcag tataaagttg cattaaacca ttcatgggat ggtggaggtg
     1741 tcccatctca aggaaatcta acgctgaact tagcaaatga ttcatatgtt acattttggt
     1801 ttgactataa tacacaatct gtaactgatt ctactaaata tactccaata gctaatgaca
     1861 aattgcccag attagttgga acaattcaat cggccattgg ggcaggtaat gattggaagc
     1921 ctgaaacatc tacagcaata atgacagatg ataattttga caacgtttat tcatatactg
     1981 cgcatgtccc aaagggtgac tatcaataca aggtgacatt aggaaataca tgggatgaaa
     2041 attatggtgc aaatggtgtg aaagatggtt caaatataca aataaatgtc acaaacgatg
     2101 ccgatataac gttttattat gatgcaaata ctcataatat ttggaccaat tatagtccca
     2161 tcctcactgg tttagataac aacatatatt atgatgacct aaagcatgat acacatgatt
     2221 cgttttttag gaatcctttt ggcgctgtaa aagtagacca aactgtcaca ttaagaattc
     2281 aagcaaaaaa tcatgatctc gagtctgcca gaatatcgta ttgggacgat ataaataaga
     2341 ttaggataga attacctatg acaagaatag gtgaaagtcc tgatggtaat tttgaatatt
     2401 gggaaataaa gttgagtttt gatcatccaa cgagaatttg gtattacttt atattaaaag
     2461 atggaactaa aacagcttat tatggcgaca atgatgatca acttggtggt gttggaaaag
     2521 caacagatac tgtaaataag gattttgaat taactgtata tgataaaaat tttgatacgc
     2581 ctgattggat gaaaggcgct gtcatgtatc aaattttccc tgacaggttt tataatggcg
     2641 atacatctaa tgatcatgca aagacattaa gccgtggcaa tgatccaata gagttccata
     2701 ataattggaa tgatctgccg gataacccta ataatgctgg aactccaggt tatactggcg
     2761 atggtatttg gtcaaacgac ttttttggtg atttaaaagg aatcgatgat aaacttgatt
     2821 atctgaaagg acttggagta tctgtaattt acttaaatcc aatatttgaa tcaccttcaa
     2881 atcataagta tgatactgct gattatacaa agattgatga gatgtttggt actacacaag
     2941 attttgaaaa gttaatgagt gatgcacatg ctaaaggtat aaaaataatt cttgatggtg
     3001 tattcaatca caccagtgat gatagtatat attttaatag atacggcaag tatccgggat
     3061 taggtgcata tcaagcttgg aaagaaggaa atcagtcttt atcgccgtat ggtgattggt
     3121 atactataaa ttccgacggt acttacgagt gctggtgggg ttatgacagt ttgcctgtca
     3181 taaaatcatt aaatggcagt gaatataacg ttacaagttg ggccaacttt ataataaacg
     3241 atgaaaatgc catttccaag tattggctaa atccagatgg gaatttaaat gatggtgctg
     3301 atggttggcg tttggatgtg gaaaatgaag ttgcacatga tttctggaca cattttagga
     3361 atgctattaa taccgtaaaa tttgaagcgc caatgatagc agaaaattgg ggagatgcgt
     3421 cccttgattt acttggcgat tcttttaatt cagttatgaa ttatcagttt agaaatgaca
     3481 tcatagattt tttgattggg caatctttcg atgatggaaa tggtcaacac aatcctatag
     3541 atgcagcaaa acttgaccaa aggctgatga gcatatatga aagatatccg ttgcctgctt
     3601 tttattcaac gatgaatttg cttggttctc atgatacaat gagaatatta acagtgtttg
     3661 gatataactc tgcagatcct aacgaaaact cagacgctgc taaacaactg gcagaacaaa
     3721 agctaaaatt agcaacaatt ttgcagatgg gatatcctgg tatggcggat atttactatg
     3781 gcgatgaagc aggagtttct ggcggtaaag atccagatga tagaagaaca ttcccgtggg
     3841 gaaatgaaga tacgacatta caagatttct ttaaaaatat atcaagcata agaaataaca
     3901 atcaagtatt aaaaactggt gatttggaga ctttatatgc acaaaatgat gtttatgcca
     3961 ttggtaggag aattataaat ggtaaagatg cttttggcac ttcataccct gatagcgctg
     4021 caattgttgc aataaataga agcaagtcag ataaacaaat agctattgat actacaaagt
     4081 ttttaagaga tggagttact tttaaagatt taattaacaa caatgtatcg tattcaataa
     4141 gtaatggaca gatagttata gatgtacctg ctatgagcgg tgtaatgctt atatcagatg
     4201 atgggcaaga tttaactgct ccacaagctc cttcaaatgt tgttgttact tccggcaatg
     4261 gtaaagttga tttatcgtgg ttgcaatctg atggtgcaac aggatacaat atatatcgtt
     4321 cgtcagtaga aggtggttta tatgaaaaaa tagcatcaaa tgtaactgaa acaacgtttg
     4381 aagatgccaa tgttacaaat ggattaaaat atgtatatgc aatatcagca attgatgaat
     4441 taggcaatga aagcggaatt agtaatgatg cagtagcata tccagcgtat cctatagggt
     4501 gggtaggaaa tcttacacaa gtatctgata atcatataat aggtgttgat aaaccaacag
     4561 aagatattta tgcggaagtt tgggcagatg gacttacaaa tagcactggt caaggtccaa
     4621 atatgatagc gcaattagga tataaatatg ttagtggaac tgtttatgat tcagtatatg
     4681 gaagtgtata taacagcgta tatggcgtag atgatagtgg ctttacctgg gtaaatgctc
     4741 aatatgtagg agacatagga aataatgatc agtacaaagc aagttttaca cctgataaaa
     4801 taggtcaatg ggaatatttg atgagatttt ccgacaatca agggcaagac tggattacca
     4861 caagcacatt atcattttat gttgttcctt ctgacgattt aataaagcca actgcacctt
     4921 atttgaatca gccaggtaca gaatcatcga gagtctcgct tacatggaat ccatctaccg
     4981 acaatgtcgg tatatacgat tatgaaattt ataggtccga tggtggaaca tttaataaaa
     5041 tagctactgt atcaaatgaa gtttataatt atattgatac gagcgtaatt aatggtgtaa
     5101 cttacaatta taaagttgtt gctgttgatc tatcatttaa taggacagaa tctaatgtgg
     5161 ttacaataaa accagatgta gtacctataa aagttatttt taatgtaact gtacctgatt
     5221 atacaccaga tgctgttaat ttggcaggaa cattccctaa tgctacttgg gatccatcag
     5281 cacaacagat gacaaaaata gataataata cttatagcat tacacttacg cttgatgaag
     5341 gaacacaaat agaatacaaa tatgccagag gtagttggga taaggttgaa aaagacgaat
     5401 acggaaatga gtttgcatca aacagaaaag taacgattgt aaatcaaggc aacaatgaaa
     5461 tgacgataaa tgatacggtt tataggtgga gggatattcc aatatttata tattctccaa
     5521 gtagcaatat gacagttgat tctaatataa gtacaatgga agtcaaaggt aatacgtata
     5581 aaggtgctaa agttacaata aatggtgata gttttgttca agataaaaat ggggtattta
     5641 ctaaagatgt ttctttaaat tatggggtaa ataagattaa gatacatgtt gaaccaaatg
     5701 atggtagtgt ttatggtaac gatcaaggca gaataactga acttacaaag gatattgaga
     5761 ttgatgtaat tagacaagag aataatagtg gaagcggaac aggaaataat aatacgagta
     5821 caagtgggag caattcaagc agcacaggca gtggtagtac tggaagtaca agtataacaa
     5881 gtaatatcag caatacaagt aatacaagta atacaatagg tgtaatcaca aagaacggca
     5941 acgttattac attgacactt gatgcaggaa aagctaaaga cctgatagta aattcaaagg
     6001 acaagaaagt cgtatttgac ataacaacaa taggtgaagg acaacaaaaa gtcgtgcaga
     6061 tttctaagga cattttagac acaagtgctg ccaacggcaa agacatcgtc ataaaatcag
     6121 acaatgcatc gatagcactc acgaaagatg cacttaatca aaaccagata caaaacggtg
     6181 tcaatgtatc aataaaagac aatggaaagc ctaatgtgac aaattatgtg tcgctttcta
     6241 atgtagtaga cataacaata agcggtatca gtgggaatgt aacattggca aaaccagtag
     6301 aggtaacatt aaatatatca aaagctaacg atccaagaaa agtagcagtt tactactaca
     6361 acccaacgac aaaccaatgg gagtacgtag ggggtaaagt agacgcatca tctggaacaa
     6421 taacattcaa tgcaacgcac ttctcacaat atgcagcgtt tgagtatgac aagacattta
     6481 atgacataaa agacaattgg gcgaaagacg taatagaagt attagcatca aggcatatag
     6541 tagaagggat gacagataca cagtatgaac caaacaagac agtgacgaga gcagaattca
     6601 cagcaatgat actgaggcta ttaaacataa aagaagaaac atacagcgga gaatttagcg
     6661 atgtaaaaag tggagactgg tatgcaaacg caatagaagc agcatacaaa gcaggaataa
     6721 tcgaaggtga cggaaagaac gcaaggccaa atgacagcat aacaagagaa gagatgacag
     6781 caatagccat gagggcatac gagatgctga cacagtacaa agaagagaat ataggtgcga
     6841 caacatttag cgacgacaaa tccataagcg attgggcaag aaatgtagtg gcaaatgcgg
     6901 cgaaattagg aatagtaaat ggtgagccaa ataacgtatt tgcacctaaa ggaaatgcca
     6961 cgagagcaga agcagcagct atcatatacg gcttattaga gaaaagcgga aacatataaa
     7021 attgtataaa ttagttatga tgagaaagat acgattttaa ttgtttactt tcatatcttt
     7081 ctcaaaatat aatttagata acttttttaa atttttttga tttaataaat aagtctattg
     7141 acttttgtaa tcaaaggata taaaatataa ttaagcaaag cgcaaccggt tgcaaatatt
     7201 ctatgaaata ctatgataag gagggatgtc tgttaacttt aaattacatg aaaacgctta
     7261 tatatgtaga taaaaagaaa aaataaaaga ctgataagat taattaaaca ttttttatca
     7321 atatggaacc gcataaattt tacaattaaa aattgcataa gtcaatagaa aaaattggct
     7381 tattgaagag gaggattttg attatgagga aaacctcaaa agtaattgcg ctgcttttag
     7441 tgcttttaat ggcattttca gtgcttataa caggatgcgg aaatagtaca aaatctgatc
     7501 aaagcaaaac ttcagaaaat agttcaactc aaaataagac tgagactaca agccaacctg
     7561 taacattaga cattttccaa tttaaagttg aaataaaaga tgctcttcaa aatgctataa
     7621 atacttacat gaaagaaaat cctaatgtta agatcaattt agaaactgta ggcggaggac
     7681 aagattacgg tgctgcatta aaagctaaat tcaattcagg ttcagagcca gcaatataca
     7741 atgtaggtgg accacaggat gttgctacat ggaagccaaa attagctgat ttgtcagata
     7801 caaaagctgc aaaagccgct cttcctggta ctcttactgg tgtgacgcag gacggtaaaa
     7861 tttatggttt accatttgat ttagagggat atggattaat ctacaacaaa cagatattca
     7921 gcaaagcagg tattgatcca tccagcatta aaacttttga tgcattggtg aatgctgtaa
     7981 agactttgga ttcaaagaag aaagagttag gcattgaagc tgttttcgca tttccggcaa
     8041 aagaaacttg ggttactgga ctccatctat ctaatgcatt cttgtcacca gaatttgatg
     8101 gagatgtcat gaaagcattt aattctaaga cagtagactt caaatatggt gatgcaatga
     8161 agcagatgat tgatcttcag aataagtatt cagtacaacc tacagcaagt cttgactaca
     8221 gcacgcaagt tgaaaagctt tttggaactg gaaaagttgc gatgattcag caaggtaact
     8281 gggtatatcc tacattagag tcacttgata agaactttgc acaaaatgat attggcatgt
     8341 tgccataccc tgttccagga tataaagaag actcatatcc cgaaggtgtc ccaatgtatt
     8401 gggcagttaa cagcgacaaa ccagcggatg ttcaaaaagc tgctaaagat ttcttagatt
     8461 ggttgtatac atcagatgaa ggaaaacaga tagttactca gcagtttaag tttgtcccag
     8521 catatactgg gtacaattca aatagcatat ctgatccgct tggaatgcag ctttttgtgg
     8581 cagcaagtca aggcaagaca tatggatggg tattcatggg atatccaaat aattggggtc
     8641 aaaatgtcct tggtgctgat atacagcttt atgtgaatgg ttcattaagt tgggataaag
     8701 tgatttcaca tgctaaacag gcttgggcag atgcgagaaa ataattaaaa attgtagtgg
     8761 gagagtataa ttgtattctc ccactttgca aatatgaaac aagaaggaag gtaatacaat
     8821 ggcaaaaaaa gcgaaatttt ttaaaaacgg gatttggtat tggcttttta tagctccaac
     8881 actattgtca cttattattg tcgtattaat accatttatt ataggaatat attattcatt
     8941 tacagattgg aatggaataa atcaacctgt atttattgga ttaaaaaatt tcatgacact
     9001 tagagacgat gctgagtttt ggaattcaat aatttttaca gctaaatttg cagtcgcgtg
     9061 tattgtgatt ataaatgtgg taggcttgag tcttgccatg cttgtaacaa gaaaaatatt
     9121 tgctagaaat ttcatgcgta cagcatttta cttgcctaat ctaataggtg gactcatatt
     9181 aggatttata tggaatttca tttttgtgga tgtgtttcaa acaatttcag acgctacaca
     9241 tattggatgg cttggaggct ggttatccac aactaatact ggcttttggg ggcttgtgat
     9301 tgtgacatca tggcaaatga taggctatgt tatggtgatt tatatagcat atattgaaag
     9361 cattcctact gatttaatag aagcttcaaa gattgatggc gcaaattcat ggcaacaatt
     9421 taggaatgtc gtatttccgc ttatagcgcc agcctttaca gtgagtttat ttattacgct
     9481 ttcaaattca tttaaactgt ttgatcaaaa cttatcatta actgctggag cgcctggcaa
     9541 tactacacaa atgataacac ttaatatata ccaaacggct ttttctgctc aagagatggc
     9601 tgtaggacag gcaaaagcgg taataatgtt cttaataatt gctgttattt ctgtcattca
     9661 agtttacttg acacagaaaa gagaggttga gatgtgatga gaaaagttca tgttcaaaaa
     9721 tatttattga catttttagg aattgtgctt tcgcttttat ggatatctcc tttttatata
     9781 atacttgtca actctttcaa gacaaaatta gagctgttta caaatacatt gtcacttcca
     9841 aaaagtttga tgctagataa ttataaaacg gcagccgcaa acttaaattt aagtgaagca
     9901 ttttcaaata cattaataat aacggttttt agcatattga ttatagcaat attttcctct
     9961 atgacggcat atgcacttca aagggtaaaa agaaaaagca gtgtaattat atatatgatt
    10021 tttacagtgg ctatgttgat cccgtttcaa tcagttatga ttccattagt agcggaattt
    10081 gggaaatttc attttcttac aaggtcaggg cttgtattta tgtacttggg atttggttca
    10141 agcttaggag tgtttttata ctatggtgca ttaaaaggaa taccgacatc tttagatgaa
    10201 gcagcattaa tagatggttg tagtagattt agaatttact ggaatatcat attgccttta
    10261 ttaaatccta ctacgattac tttggctgta ttggatatca tgtggatatg gaatgactac
    10321 ttattgccat ctttagtcat aaacaaagtg ggttccagga ctctcccatt aatgattttt
    10381 tacttcttta gtcaatacac aaagcaatgg aatctcggta tggcagggct aactatagca
    10441 attttacccg ttgtaatttt ctacttcttg gcgcagagaa aattggttac agccataata
    10501 gctggtgctg ttaaacagta agatgaatat ttgtatttaa aacaagttaa tttaaaatta
    10561 tagacgatat gcaatcgata tgaaagtatt ttgacattaa tatcgattgc ttttatataa
    10621 cagaaaggag tgattgaaaa ccataaatta ttaaacaact cgctaatcaa aaaataatta
    10681 aaaggagggt cttacatgaa aaaaacgttt aaattgatat tggtgctgat gctttcactt
    10741 acacttgttt ttggattgac agcaccaata caggcagctt ctgatacagc agtaagtaat
    10801 gttgtcaatt actcgacgga tgtaatttat cagattgtca ctgatagatt tgttgatgga
    10861 aataccagca acaatccaac tggtgatttg tatgatccaa cacatacaag tctgaaaaag
    10921 tactttggtg gagactggca gggtattatt aacaagataa atgatggtta tctaacagga
    10981 atgggtgtaa cagcaatttg gatatctcag cctgtagaaa atatttatgc agtcttacct
    11041 gattcaactt ttggtggaag tacatcgtac catggttatt gggctcgtga ttttaagaga
    11101 acaaatccat actttggaag ctttactgat tttcaaaatt tgattaatac agctcatgca
    11161 cataacataa aagtaattat cgattttgca ccaaatcata cttcacctgc atctgaaacc
    11221 gatccgactt atgctgaaaa tggaaggcta tacgacaatg gaacattact tggtgggtat
    11281 acaaatgata caaacgggta ttttcatcat tatggaggaa cagatttttc atcttatgaa
    11341 gatggcattt atcgtaactt atttgattta gcagatttaa atcaacagaa tagtacaatc
    11401 gattcatact taaaatcagc aattaaggtg tggctcgata tgggaataga cggtatacgt
    11461 ctagatgctg taaaacatat gccgtttgga tggcagaaga attttatgga tagcatattg
    11521 agctataggc ctgtatttac atttggagag tggtttcttg gaacgaatga aattgatgta
    11581 aataacacat actttgcaaa tgaaagcggt atgagtcttt tggactttag gttttctcaa
    11641 aaagtgaggc aggtatttag ggataatacg gatactatgt atggacttga ttcaatgatt
    11701 caatcaactg catctgatta taattttata aatgatatgg ttacttttat tgataatcat
    11761 gatatggata gattctataa tggtggttct actcgtccag ttgaacaagc attagcattt
    11821 actttgactt cgaggggagt acctgctata tattatggaa cagaacagta tatgacaggc
    11881 aatggagacc cttataatag agctatgatg acctcattta atacatcaac tacagcatat
    11941 aatgtaatta aaaaacttgc tcctttgcgc aaatctaatc ctgcaattgc ttatggtact
    12001 actcagcaac gttggataaa caatgatgtt tatatatatg aaagaaaatt tgggaataat
    12061 gttgctcttg ttgctataaa tagaaatctt tcaacaagtt ataatataac aggactgtat
    12121 acagcgcttc ctgctggtac ttatactgat gttcttggtg gacttttaaa tggtaatagt
    12181 atttctgtcg cgagtgatgg ttctgtaaca ccatttacac ttagtgccgg tgaagttgca
    12241 gtatggcagt atgtaagttc aagtaattct ccgttgatag gacatgttgg gccaacaatg
    12301 acaaaagcag ggcaaactat aacaatagat ggaagaggtt ttggtacgac atcaggacaa
    12361 gttttgtttg gttcgacggc tggtactatt gtctcttggg atgatacaga ggtaaaagta
    12421 aaagttcctt cggtgacacc tggtaaatat aatatatcat taaaaacttc aagtggtgct
    12481 acaagcaata cttataataa tattaacatt cttacaggca atcagatatg tgttagattt
    12541 gttgtaaata atgcaagtac agtgtatgga gagaatgttt atcttactgg taatgtagct
    12601 gaattgggaa actgggatac gtcaaaagca ataggaccaa tgtttaatca ggtagtatat
    12661 cagtatccta catggtatta tgatgtaagt gttccagcgg gtacaacaat acaatttaag
    12721 tttataaaga aaaatggcaa tacgataaca tgggaaggtg gtagtaacca tacttataca
    12781 gttccatcat ctagtacagg tactgtaatt gtaaactggc aacaatagat taaaataata
    12841 agggtcaaga gatc
//
