LOCUS       CP001656               11945 bp    DNA     linear   BCT 10-SEP-2019
DEFINITION  Paenibacillus sp. JDR-2 chromosome, complete genome.
ACCESSION   CP001656
VERSION     CP001656.1
DBLINK      BioProject: PRJNA20399
            BioSample: SAMN00000047
KEYWORDS    .
SOURCE      Paenibacillus sp. JDR-2
  ORGANISM  Paenibacillus sp. JDR-2
            Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,
            Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Ovchinnikova,G., Shanmugam,K.T., Ingram,L.O. and Preston,J.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Paenibacillus sp. JDR-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,
            Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Ovchinnikova,G., Shanmugam,K.T., Ingram,L.O. and Preston,J.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Metadata-START##
            Organism Display Name :: Paenibacillus sp. JDR-2
            GOLD Stamp ID         :: Gi02023
            Greengenes ID         :: 270979
            Isolation Site        :: Sweet gum stem wood buried in surface soil
            Oxygen Requirement    :: Aerobe
            Cell Shape            :: Rod-shaped
            Sporulation           :: Sporulating
            Temperature Range     :: Mesophile
            Temperature Optimum   :: 30 C
            Gram Staining         :: gram+
            Biotic Relationship   :: Free living
            Diseases              :: None
            Habitat               :: Host, Wood
            Phenotypes            :: Methylglucuronoxylan utilization
            ##Metadata-END##
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4043135
            Source DNA and bacteria available from James Preston
            (jpreston@ufl.edu)
            Contacts: James Preston (jpreston@ufl.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Paenibacillus sp. JDR-2"
                     /mol_type="genomic DNA"
                     /strain="JDR-2"
                     /db_xref="taxon:324057"
     gene            1..1569
                     /locus_tag="Pjdr2_1318"
     CDS             1..1569
                     /locus_tag="Pjdr2_1318"
                     /inference="protein motif:PFAM:PF00072"
                     /note="PFAM: response regulator receiver; helix-turn-helix-
                     domain containing protein AraC type; SMART: response
                     regulator receiver; helix-turn-helix- domain containing
                     protein AraC type; KEGG: bha:BH2109 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0036286,DBD-SUPERFAMILY|0035607"
                     /protein_id="ACS99993.1"
                     /db_xref="InterPro:IPR000005"
                     /db_xref="InterPro:IPR001789"
                     /translation="MYKVLLVDDEIFVRKGLRNLIDWESLGYEICDEAGNGQEALEKIQ
                     LIKPDLVIADIRMPVLDGLELIRKVTEEGVHSPTFIIVSGYHDFQYAQRALRYGVHDYI
                     LKPIDEAELETTLKALSGTLGLKKLATIAGDSLVTDSIVETLVQGQFSEADNGALCAAL
                     QMPEFSSFDYVLVELHASAQSAGTEWQAKELAAVVQSVSATPSSKIPVYEQTSGLFGLL
                     IDRKPFLPPEIRLESAYNSLHAAIAKSSGRPVTLYIGKTVERLQEMCLSYRAANEAMSY
                     KYAEQGSSVIYANQVQGTPLYYFDVDSELYSRLLERLEENNADLYEQDIELLFRQFVEK
                     RFAPNAVANTITRFVIGIINIIRKMEGDEKGLSKLPFIMDWQGSNSRLQDLKGLFTDFL
                     REAAVYLAELRNEQSKGGIERIKKYIEANYTENISLKSIAGKFYMNSVYLGQLFRKTYG
                     IYFNDFLLQIRIGEAKKLLRQTDLRMYEIAEKVGFQNADYFVTQFEKLEKVTPTDYRNK
                     LLGKK"
     gene            1585..3303
                     /locus_tag="Pjdr2_1319"
     CDS             1585..3303
                     /locus_tag="Pjdr2_1319"
                     /inference="protein motif:PFAM:PF06580"
                     /note="PFAM: histidine kinase internal region; ATP-binding
                     region ATPase domain protein; histidine kinase HAMP region
                     domain protein; SMART: histidine kinase HAMP region domain
                     protein; KEGG: bha:BH2110 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|F4LXP4|8.A.59.2.1"
                     /protein_id="ACS99994.1"
                     /db_xref="InterPro:IPR003594"
                     /db_xref="InterPro:IPR003660"
                     /db_xref="InterPro:IPR010559"
                     /translation="MGRFHWNHLKLRDKLLLMYVFCVFIPIVLTNIVFYNVTTNNIKNQ
                     KSHDADIALEKLQGELRAVIDEAAGISYLYYIDPMLNQLLDKKYDSQIEYVEAFNNIRS
                     VFNKSEQAYKTTSATVIYTDNPTVLSSGPIMPLSETEKEADWYRAFMKTNVSYPLFIQD
                     GDTFSLVQRLNYVKGNGYNNLIKIDLNMATVKQLFALSGFEGSIYFLNPEGAVLYSNDS
                     SVDRHSQTLPMPKNSLSFDKVYTNNNYLNDWSLHGVINEGSFLHDVRKSGSFVIFLALI
                     NFVLPSLIIAALSRSINKRLVKIVKHMKKVKNQHFETIPLDDARDEIGQLTGEFNRMTE
                     RIDNLITDVYQADIQKKDLEIRQRQAQLHALHSQINPHFLFNSLETIRMRSLMKGETET
                     AKTIHYMAKIFRKSISWKRSWVSIREEIELTECFLEIQKYRFGDKLQYQITVEDTVYDQ
                     MIPKMTFLPFVENASIHGIESSPGIGLIQIHIGIAGNKLMFRLSDNGIGMSQAKLSELL
                     NYLRLDDSIGDNVGMKNVYTRLKLCYKDSFEFDIASEEGHGTTVELRLPLDMNGQ"
     gene            3437..5149
                     /locus_tag="Pjdr2_1320"
     CDS             3437..5149
                     /locus_tag="Pjdr2_1320"
                     /inference="protein motif:PFAM:PF01547"
                     /note="PFAM: extracellular solute-binding protein family 1;
                     KEGG: bha:BH2111 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A9QDR6|3.A.1.1.29"
                     /protein_id="ACS99995.1"
                     /db_xref="InterPro:IPR006059"
                     /translation="MVNKKLLTVSLAAAMLAVTASACGGNNDNSNANSGKSGNSGNAAS
                     NSASPEADDKTPVTFSYYSFTSQKDALASDTVIGKELQQQTGVDWKMEFLVGDPQTKSG
                     VMIASGDYPDVIVPEGEIDKLLDAGAFIPLDDLIEKYGPNIKRVYGPYFDKFRQADGKM
                     YFLPFGANQGYIGDPGISQGAFWIQRSVLKEAGYPKIKTLDQYFDLIKQYQEKHPQVDG
                     KDTIGFASLAGPADSFFSITNPSMHLAGYPNDGDVIVDMNTHEAKTYAATDIEKKWLQK
                     LNEVNAEGLFDPETFTANKDQFLAKLTSGRVLGYFNYAWQVGDATNNLKKAGIDEKRYA
                     PLPIVFDENTKDQYVDPPSFVNNRGIGISVKAKDAVRIIKYFDNLLKEENQVLVQWGVK
                     DQNYTVDANGRYVMDAQQIADRNDPEKKRTVGWQYFEYSWPRYGNNSVLADGNSYGVGN
                     QPEVAYAGYTDGDKALLDAYGVKTFSEYFSKPDDRSWYPAWSINKGQGTPEQIFQQKAG
                     DLQKKFVPKLVLAKPSEFDSIWSDYTGQMGKLDVKGYETFVTKVVQDRIAGKW"
     sig_peptide     3437..3505
                     /locus_tag="Pjdr2_1320"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 1.000) with cleavage site probability 0.754 at
                     residue 23"
     gene            5239..6210
                     /locus_tag="Pjdr2_1321"
     CDS             5239..6210
                     /locus_tag="Pjdr2_1321"
                     /inference="protein motif:PFAM:PF00528"
                     /note="PFAM: binding-protein-dependent transport systems
                     inner membrane component; KEGG: bha:BH2112 transmembrane
                     lipoprotein"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A9QDR7|3.A.1.1.29"
                     /protein_id="ACS99996.1"
                     /db_xref="InterPro:IPR000515"
                     /translation="MENIHEVTPSVVRSPKASESRLQLFFKKLLQQKVLVFMSMPFVLW
                     LFLFKYVPLWGWTISFQKFRPAKDLFDQEWVGWKNFNFLFNDDSFYRVLRNTIVMSSIN
                     LVLGFVTAIVLAILLNELRQIMFKRVVQTISYLPHFISWVVAANIISSALAPEGIVNIL
                     LTRMHLIDQPILWLGKGNYFWGILGASEVWKNVGWNTIIYLAAITTIDPSQYEAAEIDG
                     ANRLQRILHITLPGLKSVIVILLIMNLGNILESGFEPQYLLGNGMTVDYSENLDIFVLK
                     YGMNMGNYSLATAAGMFKTVVSFIFLLSANSIAKRLGESRLF"
     gene            6250..7170
                     /locus_tag="Pjdr2_1322"
     CDS             6250..7170
                     /locus_tag="Pjdr2_1322"
                     /inference="protein motif:PFAM:PF00528"
                     /note="PFAM: binding-protein-dependent transport systems
                     inner membrane component; KEGG: bha:BH2113 ABC transporter
                     (permease)"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A9QDR8|3.A.1.1.29"
                     /protein_id="ACS99997.1"
                     /db_xref="InterPro:IPR000515"
                     /translation="MKESSAKARYSSLPDKIFDTSNIVFMLLVVTVTLYPFLNMFALSF
                     NDANDSIRGGIYAWPRMWTWDNYSYIFNEASIYHATLISALRTIAGTITSVFCTAMLAY
                     TISRQEFVLRKFVTLVAIFTMYFSGGLIPGYLLIKELHMINSFWVYIIPGIIGVFNMIV
                     IRSFIEGLPDGIMESAKIDGAGEFITFMRIVLPLTVPALATVSLFVAVSQWNSWFDVFL
                     YNSSHLNLSTLQYELMKILQTSNTAASSTNAGDQFAAGQSGVTAVTPTSIRATMTIVAS
                     LPIILVYPFLQKYFVKGMTVGGVKG"
     gene            7267..9330
                     /locus_tag="Pjdr2_1323"
     CDS             7267..9330
                     /locus_tag="Pjdr2_1323"
                     /EC_number="3.2.1.139"
                     /inference="protein motif:PRIAM:3.2.1.139"
                     /note="PFAM: Glycosyl hydrolase 67 middle domain protein;
                     glycosyl hydrolase 67 C-teriminal domain protein; glycoside
                     hydrolase family 67; KEGG: bha:BH1061 alpha-glucuronidase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH67"
                     /protein_id="ACS99998.1"
                     /db_xref="InterPro:IPR005154"
                     /db_xref="InterPro:IPR011099"
                     /db_xref="InterPro:IPR011100"
                     /translation="MGDNGYAAWLRYDQVKDETRLEQYAGWTGELVLPAGVPMEGIMKT
                     AAVELSRGIRSMLGTTPSVTHEASGQRFIVLEVLGGGSWIDQAAGDAAALSDEGYFLKT
                     VREAEKEYIIAAGKSEKAVLYAVFHLLRLMQSGTAIDQLNLVESPKYSLRMINHWDNMD
                     GSVERGYSGRSIFYDNNKVLSDSERIRDYARLMASVGINGIAINNVNVHREETFLITEK
                     LLPDVVRIAEVFGEYGIKLFLSVNYAGTIEIGGLETADPLDPAVRGWWKEKAAEVYRYI
                     PDFGGFLVKADSENRPGPFTYGRDHADGANMLAEALEPFGGLVLWRCFVYNCHQDWRDR
                     STDRARAAYDHFKPLDGRFKDNVILQIKNGPMDFQVREPVSPLFGAMEHTNQMMEFQIA
                     QEYTGQQKDVCFLIPQWKEVLNFDTYVKGAGSTVKEIAAGSVHAYTHSGITAVSNIGND
                     ENWTGHHLAQANLYGYGRLIWNPELSSEEIAAEWAAQTFGGNKNVQDVLVNFLLESLSI
                     YENYTAPLGVGWMVTPHYHYGPDIDGYEYSKWGTYHFADHKGIGVDRTAQTGTGYSSQY
                     ALPNAEVYDSLEACPDELLLFFHHVPYTHQLKSGKTVIQHIYDTHFAGVERVEYWMNRW
                     QELEGLVDGDRFRHVTGRMNWQRENAKQWRDIVNTYFFRKSGIPDTHNRVIY"
     gene            9358..10383
                     /locus_tag="Pjdr2_1324"
     CDS             9358..10383
                     /locus_tag="Pjdr2_1324"
                     /EC_number="3.2.1.8"
                     /inference="protein motif:PRIAM:3.2.1.8"
                     /note="KEGG: pat:Patl_2657 endo-1,4-beta-xylanase; PFAM:
                     glycoside hydrolase family 10; SMART: glycoside hydrolase
                     family 10"
                     /codon_start=1
                     /transl_table=11
                     /product="GH10"
                     /protein_id="ACS99999.1"
                     /db_xref="InterPro:IPR001000"
                     /translation="MSYTSELPALRSVYKDYFDIGAAVNLTTIASQKDVLTAHYNSLTA
                     ENDMKFERVHPQEGQYTFEAADKIADFAAANAMKLRGHTLVWHNQTPDWIFQNANGSPV
                     DRETLLARMKSHIEKVVGRYKGIIYGWDVVNEVIDDKNGVWLRESKWLNLAGEDFIAKA
                     FEYAHAADPKALLFYNDYNECIPEKRDKIIRIVQSLQAKQVPIHGIGLQGHWNLNGPSL
                     AEIREAIERYAATGLKLQVTELDISVFDHDDKRTDLTEPTADMLERQAERYGQVFELFR
                     EYKEAITAVTFWGAADDYTWLDNFPVRGRKNWPFVFDANHEPKASFRKITDWQEAGQID
                     S"
     gene            10380..11945
                     /locus_tag="Pjdr2_1325"
     CDS             10380..11945
                     /locus_tag="Pjdr2_1325"
                     /EC_number="3.2.1.37"
                     /inference="protein motif:PRIAM:3.2.1.37"
                     /note="PFAM: glycoside hydrolase family 43; KEGG:
                     sde:Sde_1655 translation initiation factor IF-1"
                     /codon_start=1
                     /transl_table=11
                     /product="GH43_12"
                     /protein_id="ACT00001.1"
                     /db_xref="InterPro:IPR006710"
                     /translation="MTYASTGKITYRNPVLPGFYPDPSAVRVGEDYYMVTSTFEYFPGV
                     PVFRSKDLVHWEQIGHVLTRESQVNLLSRNSSEGIYAPALRYHEGVFYMITTDVYGIGN
                     FYVTATDPAGPWSDPIRIPYGNIDPSLFFDDDGKAYVSAQAGFGETSHIIQYEIDILTG
                     EALSEPVVVFEGDEGPWVEGPHLYKINGFYYMMTASGGTGPMHREIIGRGTSPYGPFEM
                     LPHPILTHNELQDHPIQYTGHVELLDDVRGQWWALFLGVRPQGGNGSVLGRETFLAPVR
                     WSEDGWPMIDNNEGHVSMVMEGPAIRESKTSMAGMDKSVTTKFTSEGLSLEWMYNRVIP
                     GPEELSLTEKEGCLRLSGNAKGLRDGGANVFVCRRQQHHRMSIETRLTFVPGREGEQAG
                     IAARLSDKSHYSIGITKKDGQTGILVTAMAQGSGTEVFTPIEEQVPVRLSIRSDEKEYE
                     LLYTLGGASAEWVSLRTLPVEALTPDADGAFTGVCLGMFAAGTEEGHSAPAYYDYFEYR
                     PESG"
ORIGIN
        1 atgtacaaag tgttattggt ggatgatgag atttttgtgc gcaaagggtt aaggaattta
       61 atagactggg aatcgcttgg ctatgaaatt tgcgatgaag cgggtaacgg acaggaagca
      121 ttagagaaaa tacagctgat taagcctgat ctcgttatcg cggatatccg gatgccagtg
      181 ctggacggat tggagcttat ccgcaaggtt accgaggagg gcgtacatag cccgaccttt
      241 attatcgtca gcgggtatca tgatttccag tacgcgcagc gagctctccg ttacggagtc
      301 catgactata tattaaagcc tatcgatgaa gcggaactgg agacaacgtt gaaggcgctt
      361 tccggaacgt tgggattaaa aaagctggcc acgatcgcgg gggacagcct ggtcacggac
      421 tctattgtgg aaacgcttgt tcaaggccag ttctcggagg ccgataacgg agctttatgc
      481 gcggcgctgc aaatgcctga gttctccagc ttcgattacg tgctggttga gcttcatgcc
      541 agcgcgcaat ccgcaggcac cgaatggcag gccaaggagc tggcagcagt cgtgcagtca
      601 gtgagcgcta ctccaagcag caaaattcct gtctatgagc agacgagcgg tttatttggc
      661 ctgcttattg atcggaagcc gtttttaccg ccggaaatac gacttgaatc tgcctataac
      721 agcttgcatg cggccattgc gaaatcttcg ggcagaccgg tcaccttata tatcggcaag
      781 accgtggaac gactgcaaga aatgtgccta tcctaccggg cggcgaatga agccatgtcc
      841 tataaatacg ccgagcaagg cagctccgtt atctatgcga atcaggtaca ggggacccct
      901 ctgtattatt tcgatgtgga ttcggagctg tacagccgac tactcgagag actggaagag
      961 aataacgcgg atttgtatga gcaggatatc gagcttttat tccgccaatt tgtcgagaag
     1021 cggtttgctc ccaatgcggt tgccaatacg attacccggt ttgtgatcgg gattattaat
     1081 ataattcgga agatggaggg agacgagaag ggacttagca agctcccgtt cattatggac
     1141 tggcagggca gcaatagccg tctgcaggac ttgaagggac tattcacgga tttcctccgc
     1201 gaggctgccg tctatttggc agagcttcgg aacgagcagt ccaagggcgg gattgaacgc
     1261 atcaagaaat acatcgaagc gaactatacg gagaatatta gccttaagag cattgcagga
     1321 aaattttata tgaactccgt ttatttgggt cagctgttcc gtaaaaccta tgggatctat
     1381 ttcaatgatt ttctactgca gatccggatc ggagaagcca aaaagctgct tcgccagacc
     1441 gatcttagga tgtacgagat tgcggagaaa gtgggctttc agaatgccga ttatttcgtg
     1501 acccaatttg agaagctgga gaaagtgaca ccgaccgatt accgcaacaa gctgctgggc
     1561 aaaaaataaa cgggtggaga tttgatgggc agatttcatt ggaatcatct taaattgcgg
     1621 gacaagcttt tgctgatgta cgtcttttgc gtatttatcc cgatcgtatt gacgaatatc
     1681 gtcttctata acgttacaac caacaatatt aaaaaccaaa aatcgcatga cgccgacatc
     1741 gccctggaga agcttcaggg cgagcttcgt gctgtcatcg atgaagccgc gggtatttcc
     1801 tacctgtatt atatcgatcc gatgctgaat cagctgctgg ataaaaagta cgattcccaa
     1861 atcgaatacg ttgaagcttt taataatatc cgaagcgtct tcaacaaatc cgagcaggct
     1921 tacaaaacga ccagcgctac ggttatttat accgataatc caactgtgtt atcctccgga
     1981 cctattatgc cgcttagcga aacggagaag gaagccgact ggtaccgggc ttttatgaag
     2041 acgaatgttt cttatccgtt gtttatacag gatggagaca cgtttagcct tgttcaaaga
     2101 ctgaactatg tcaaaggcaa cggctataac aatctgatca agattgatct gaatatggca
     2161 acggtcaagc agttgtttgc gttatccggc tttgagggca gcatttactt cttgaatccg
     2221 gaaggtgccg tcctctattc caacgactcc agcgtagaca ggcattcaca gacgcttccg
     2281 atgccgaaaa actctctctc ctttgacaag gtttacacga acaacaatta tttgaacgac
     2341 tggtccctgc atggcgtcat taacgaaggg agctttctgc atgacgtgcg gaaatcgggt
     2401 tcctttgtca tttttttggc gcttattaac tttgtgctgc cttctttgat tatcgcggct
     2461 ctctccagat ccataaacaa acgtctcgtc aagattgtaa agcatatgaa aaaggtgaaa
     2521 aaccagcatt tcgagacgat cccgcttgat gatgcgcgcg atgagatcgg ccaattaacg
     2581 ggtgaattca accggatgac cgagcggatc gacaacctca ttaccgatgt ctatcaggct
     2641 gatattcaga agaaggacct ggagatccgc cagcgccagg cacagcttca tgcccttcac
     2701 agccagatca atccccactt cctgttcaac tcgctcgaga cgattcggat gcggagcctg
     2761 atgaaggggg agaccgagac cgcgaagacc attcactata tggcgaagat tttccggaag
     2821 tcgatttcct ggaaacgcag ctgggtgtcg atccgggaag agattgagct gacggaatgc
     2881 ttccttgaaa ttcagaaata caggtttggc gacaagctgc aataccagat caccgtcgaa
     2941 gataccgtat acgaccagat gattccgaag atgaccttcc tgccgtttgt cgagaacgcc
     3001 agcattcatg gcattgaaag ctcgccggga atcggtctta ttcagattca tatcggaatc
     3061 gccggcaaca agctgatgtt caggctgtcc gataacggga tcggcatgtc ccaagccaag
     3121 ctgtcggagc tgcttaatta cctgcgtctg gatgattcca tcggtgacaa tgtcggcatg
     3181 aaaaacgtct atacccggct gaagctatgc tataaagact cgtttgaatt cgacattgct
     3241 agcgaagaag gtcatggaac aaccgttgag ctgcgtcttc ctctcgacat gaacgggcaa
     3301 taaatgacaa aaaagttgaa ctatgctttt caaagtacga actaaagttt gaggggtaat
     3361 caagccttcg gattcgtttt atgatgaaag tgcttacaac aaacacgttg aaatgacgaa
     3421 aggggtctaa acagaaatgg ttaacaaaaa gcttttaact gtcagcttgg ctgctgctat
     3481 gttagctgtt acagcatccg cttgcggcgg caataacgat aacagcaatg caaatagcgg
     3541 taaatccggc aatagcggca acgcagcttc caactccgct tctcctgaag cggatgacaa
     3601 aacacccgtc actttctcgt actacagctt caccagccaa aaggatgctc ttgcgagcga
     3661 caccgttatc ggtaaagagc tgcaacagca gaccggcgta gactggaaaa tggaattcct
     3721 cgttggcgat ccgcaaacga aatccggcgt tatgatcgca agcggcgact atccggacgt
     3781 aatcgttccg gaaggcgaaa tcgacaagct gcttgacgct ggcgcattta ttcctcttga
     3841 tgatctgatc gagaaatacg gtccaaacat caagcgcgta tacggtcctt attttgataa
     3901 attcagacaa gcagacggca aaatgtactt cctgccgttt ggcgcgaacc aaggctatat
     3961 cggcgatccc ggcatcagcc aaggcgcatt ctggatccaa cgctccgttc tgaaggaagc
     4021 gggatatccg aagatcaaga cgcttgacca gtacttcgat ctgatcaagc aataccaaga
     4081 gaagcatccg caagtagacg gcaaggacac gattggcttt gcatcccttg caggtcctgc
     4141 cgacagcttc ttctcgatca ccaacccttc catgcacctt gcgggctacc cgaatgacgg
     4201 cgacgttatc gtcgacatga acacgcatga ggctaaaact tacgcagcta cggatatcga
     4261 gaaaaaatgg ctgcaaaagc tgaacgaagt aaacgcggaa ggcttgttcg atcctgagac
     4321 ctttacagcg aacaaagacc aattcctggc taagctgacc tccggccgcg tacttggcta
     4381 cttcaactac gcatggcagg ttggcgacgc gacaaacaac ctgaagaaag ccggcatcga
     4441 cgagaagcgt tacgctccgc tgccaatcgt attcgacgag aatacgaaag accaatacgt
     4501 agatccgcct agctttgtta acaaccgcgg tatcggtatc tcggtaaaag cgaaagacgc
     4561 tgtacgcatt attaagtact tcgacaacct tctcaaagaa gagaaccaag ttctcgtaca
     4621 gtggggcgtt aaagaccaga actacaccgt ggacgctaac ggccgttacg tgatggatgc
     4681 gcagcaaatc gctgaccgca acgaccctga gaagaaacgc acagtcggat ggcagtactt
     4741 tgaatacagc tggccgcgtt acggcaacaa ctccgtactg gctgacggca actcgtatgg
     4801 cgtaggcaac cagcctgaag tggcttacgc gggttacacg gacggcgaca aagcgctgct
     4861 ggacgcctac ggcgtgaaga ccttctccga atacttctcg aagcctgacg atcgctcctg
     4921 gtacccggca tggagtatca acaaaggtca aggcacgcct gagcagatct tccagcaaaa
     4981 agccggcgac ctgcaaaaga aattcgttcc gaagcttgta cttgccaagc ctagcgaatt
     5041 cgactcgatc tggagcgact atacaggcca aatgggcaag cttgacgtga aaggctatga
     5101 aaccttcgtt acgaaagtag ttcaagaccg tatcgcgggc aagtggtaag caaataaaag
     5161 tagacggaag gccggaaagc tgattccggc cttccgatct attggattca tgcaggatgc
     5221 tagagagggg aagagcaagt ggagaacata catgaagtta caccatctgt tgtcaggagt
     5281 cccaaagcaa gcgaatcacg gcttcaactg tttttcaaaa agttattgca gcaaaaagtg
     5341 ctcgtgttta tgtcgatgcc gtttgtatta tggcttttcc tgtttaagta cgtaccgctc
     5401 tggggctgga ccatctcctt tcaaaagttc agaccggcaa aggatttgtt cgatcaggag
     5461 tgggtaggct ggaaaaactt caacttcctg tttaacgacg actccttcta tcgcgtgtta
     5521 agaaatacga tcgttatgag ctccatcaat cttgtattag gttttgttac cgcaatcgta
     5581 ttggcgattt tattaaacga gcttcgccaa attatgttca agcgggtcgt acagacgatc
     5641 agctacctac cgcattttat ttcctgggtc gttgcggcca atattatttc ttcggcgctt
     5701 gcgcccgaag ggatcgttaa tattctattg acgagaatgc atctgattga tcagcctatc
     5761 ttatggctgg gcaaagggaa ttacttctgg ggcatccttg gcgcatcgga agtttggaaa
     5821 aacgtcggct ggaacaccat tatctatttg gcggctatta caaccattga cccttcccaa
     5881 tacgaagctg ccgagattga cggggcgaac cgtttacaac ggatcctgca tattacttta
     5941 ccgggtctga agtcggttat cgtaatcctg ttgatcatga accttggaaa tattctggaa
     6001 tcgggatttg agccgcaata cttgctcggc aacgggatga ccgtggacta ttccgagaac
     6061 cttgatatat ttgtgttgaa atacggcatg aacatgggca actattcctt ggctacagca
     6121 gccggcatgt tcaaaacggt cgttagcttt atcttcctct tatcggccaa ctcgattgcg
     6181 aagcggcttg gcgagagcag actgttctag atttaaccga aagttggacg aagcaaggag
     6241 gctatcgaca tgaaagagag ttcggccaaa gccaggtaca gctctttacc ggataagatt
     6301 tttgatacaa gtaatatagt ttttatgttg ctcgtcgtga cggttacgct atatcctttc
     6361 cttaatatgt tcgcgttgtc gttcaacgat gcgaatgact cgatccgcgg cggcatttat
     6421 gcttggccaa ggatgtggac atgggataac tacagctata tttttaatga agcatccatc
     6481 tatcacgcta cgttgatctc cgccttgcgt acgattgccg gcacgattac ttccgtcttc
     6541 tgtacggcta tgctggctta tacgatcagc cgccaggagt ttgttctgcg caaattcgtt
     6601 acgctggtcg caatcttcac gatgtacttc agcggaggtc tcattccggg atacctgctg
     6661 atcaaagagc tccatatgat taactcgttc tgggtttata ttatccccgg cattatcggc
     6721 gtctttaata tgatcgtaat ccgatccttt atcgagggat tgccggacgg cattatggaa
     6781 tcggcgaaaa ttgacggagc gggcgaattc attaccttca tgcgaattgt tttgccgctg
     6841 accgtgcctg ctcttgcgac cgtttcgctc ttcgtagcgg tgtctcaatg gaattcctgg
     6901 tttgacgtat ttctgtacaa ctcttcgcat cttaacctga gcaccttgca atacgaatta
     6961 atgaaaatat tgcaaacctc caatacggcg gcatcgtcaa ccaatgcagg cgatcagttt
     7021 gcagccggac aaagcggcgt aacggcggtt acgccaacct cgatccgtgc gacgatgact
     7081 attgttgcga gtctgccgat tattctggta tatccgttcc tgcagaaata ctttgttaaa
     7141 gggatgaccg taggcggcgt taaaggttaa tcgcttgaat cacgcgtccc tcgactttgc
     7201 atcgggcgga cgcttttttt ctaattttgg ctgtttgtat aacaattctt attaggggga
     7261 ttagagatgg gagacaacgg atacgcggca tggctgagat acgatcaagt gaaggatgaa
     7321 acgcgcttgg agcaatacgc aggctggacg ggtgagctcg tgctgcccgc gggagtaccg
     7381 atggaaggga tcatgaagac agccgccgtg gaattgtcgc ggggaatccg ctccatgctg
     7441 ggcacaacgc cttcggttac gcatgaagca tcagggcagc gttttatcgt gctggaggtt
     7501 cttggcggcg gctcttggat agatcaagcg gccggcgatg cggcggctct atctgatgag
     7561 ggctacttct taaaaaccgt tcgagaagcg gagaaggaat atattatcgc ggccggcaag
     7621 tccgagaaag cagtactgta cgcggtattc catctgctgc gcctgatgca gtccgggact
     7681 gcgattgatc agttaaatct ggtcgagtcg ccgaaataca gccttcgcat gattaaccat
     7741 tgggataaca tggacggcag cgtggagcgc ggttactccg gccgttcgat tttttacgat
     7801 aacaacaaag tgctgtccga ttcggaacgg attcgcgatt acgcccgtct gatggcatcg
     7861 gtcggaatta atggcatcgc gattaataac gtaaacgtcc accgcgaaga gacattcctt
     7921 attacggaga agcttctgcc ggatgttgtc cgtatcgccg aggtatttgg cgaatacggc
     7981 ataaagctgt tcctgagcgt gaactatgcc ggaaccattg agattggcgg cctggagaca
     8041 gccgatcctc ttgatccggc cgtgcgcggc tggtggaagg agaaggcggc tgaagtgtac
     8101 cgttatattc cggactttgg gggcttcctt gtgaaagccg attccgagaa tcgccccggt
     8161 ccgtttacct acggacgcga ccatgcggac ggagctaaca tgctggctga agcattggag
     8221 ccgtttggcg gacttgtgct ctggcggtgc ttcgtttaca actgccatca ggactggcgg
     8281 gatcgctcaa ccgaccgtgc gcgtgccgct tacgatcact ttaagccgct ggacggacgc
     8341 ttcaaggaca atgtgatttt gcagattaag aacggtccga tggacttcca ggtccgcgag
     8401 ccggtctcgc cgctctttgg cgcgatggag cataccaacc agatgatgga attccagatt
     8461 gcccaggaat atacgggcca gcagaaggat gtctgcttcc tcattccgca gtggaaggaa
     8521 gtcttgaact ttgacactta cgtcaaaggt gcgggcagca cggtgaagga gattgcggca
     8581 ggcagcgtcc atgcctatac tcatagcggc attacggccg tcagcaatat cggcaacgac
     8641 gagaactgga ccgggcatca tctcgcgcag gcgaatttgt acggctacgg acgcttaatc
     8701 tggaatccgg agctttcttc ggaggagatt gcggcggaat gggctgccca aacgtttggc
     8761 ggcaataaga acgtacagga tgttcttgta aacttcctgc tggagtcgtt gtccatttac
     8821 gagaattata cagcgccgct aggcgtaggc tggatggtta cgcctcatta tcactacggt
     8881 ccggatattg acggctacga atattccaag tggggaacat accattttgc cgatcataag
     8941 ggaattgggg ttgaccgtac ggctcagacg ggtaccggct acagcagcca gtatgctttg
     9001 ccgaacgccg aggtctacga cagcctggag gcttgtccgg atgagctgct gctcttcttc
     9061 catcatgtgc cttacacgca tcagttgaaa tccggcaaga cggttattca gcatatttac
     9121 gatacgcatt tcgccggcgt ggagcgggtt gaatattgga tgaaccgctg gcaggagctt
     9181 gagggtctcg tggacggcga ccggttccgc catgtaacgg gacgcatgaa ctggcagcgt
     9241 gaaaatgcca agcaatggcg cgacatcgtt aatacgtatt tcttccgcaa atccggtatt
     9301 ccggataccc ataaccgggt gatttactaa tctacaagga aagagttggt gcaggatatg
     9361 tcatatactt cggagttgcc ggcacttcgt tccgtttata aggattactt tgatattggg
     9421 gcagctgtga atctgacgac aattgcatcc cagaaggatg ttcttactgc ccattacaac
     9481 agtctgactg ccgaaaatga catgaagttc gaacgcgtgc atccgcagga agggcagtat
     9541 acgttcgaag cggcggacaa gatcgctgac tttgcggctg caaacgccat gaagctgcgc
     9601 gggcatacgc tggtgtggca caatcagacc cctgactgga tattccaaaa tgcgaatgga
     9661 tcgccagtcg atagggaaac gctgcttgcc cggatgaaaa gccatatcga aaaggttgtt
     9721 ggccggtata aaggcattat ttacggctgg gatgttgtga acgaggttat cgacgataaa
     9781 aacggtgtct ggcttaggga gtccaaatgg ctaaacttag ccggggaaga ttttatcgca
     9841 aaagccttcg agtatgcgca tgccgccgac ccgaaagcgc ttctgttcta caatgattac
     9901 aacgagtgca ttccggagaa gcgggataaa attatccgta tcgttcaatc gctgcaggct
     9961 aagcaagtgc cgattcacgg tatcggcctg caggggcatt ggaatttgaa cggtccaagt
    10021 cttgccgaga tccgggaagc catcgaacgt tacgcggcaa cgggtcttaa gctgcaggta
    10081 acggagctgg atatttcggt attcgatcat gacgataagc ggacggatct gacggagcct
    10141 acggcagaca tgctggagcg gcaggcggaa cgttacgggc aggtattcga gctgttccgc
    10201 gaatacaaag aggcgattac cgccgttacg ttctggggcg cagccgacga ttacacctgg
    10261 ctggataact tcccggtacg cgggcggaaa aattggccgt ttgtgtttga tgccaaccac
    10321 gagcctaaag cttccttccg gaaaataacc gattggcagg aagcggggca aatcgattca
    10381 tgacatacgc atcaacaggc aaaatcacgt acaggaaccc ggtactgccg ggcttttatc
    10441 cggatccaag cgctgttcgg gtaggcgaag attattacat ggtgaccagc acctttgaat
    10501 attttccggg agttccggtg tttcgcagca aggatctcgt tcattgggag cagattggtc
    10561 atgtgttaac cagggaaagc caagtgaatc tgctgagccg caacagctcg gagggcatct
    10621 atgcgcctgc tcttcgttat catgaaggcg tattttatat gattacaacg gatgtgtacg
    10681 ggataggcaa cttctatgta accgcaacgg atccggcagg accatggtcc gatccgattc
    10741 ggattcctta cggcaatatc gatccttctc tgttttttga cgatgacggc aaagcgtacg
    10801 tctcggcgca ggccggcttt ggcgagacgt ctcatattat ccaatacgag attgatatcc
    10861 tgacgggcga ggccttatcg gagcctgttg ttgtatttga gggagacgaa gggccttggg
    10921 tagaagggcc gcatctgtac aaaataaacg gcttttatta tatgatgacc gcatccggcg
    10981 gaacggggcc gatgcacagg gagattatcg gccgcggcac ctctccttac ggaccgtttg
    11041 aaatgctgcc gcatccgatt ctgacgcata acgagttaca ggatcatccg attcaataca
    11101 ccggccatgt tgagctgctc gatgacgttc gtggccagtg gtgggcattg tttcttggcg
    11161 tccggccgca aggcggcaac ggcagcgtgc ttggccgcga gacgttcctt gctcccgtac
    11221 gctggagtga agacggctgg ccgatgattg ataataacga aggtcatgta tcaatggtta
    11281 tggagggacc ggctatccgg gagagcaaaa cctccatggc cggcatggac aagtcggtga
    11341 ccaccaagtt cacttccgaa gggcttagtc tcgaatggat gtataaccgg gtgatccccg
    11401 gaccggagga gctgtcttta acggagaagg aaggctgcct tagactaagc ggcaatgcca
    11461 aaggtcttcg cgacggagga gctaatgtat tcgtatgccg ccgccagcag catcatcgga
    11521 tgagcatcga gactcgcctg acctttgttc cgggaagaga aggggagcag gcaggaattg
    11581 ccgcgcggct gagtgacaag tcccattact cgatcggaat cacgaagaag gacggacaaa
    11641 ccggcattct tgtaacggcg atggcgcaag gaagcggaac ggaagtcttt accccaattg
    11701 aggagcaggt tcccgtccgg ctctcgatcc gttcggacga gaaggaatac gagcttttgt
    11761 ataccttggg aggtgcgtcc gcggagtggg tcagcctaag gaccttgccg gtggaagcct
    11821 taacgccgga tgcggacgga gcttttacgg gcgtatgtct tggcatgttt gcagcaggaa
    11881 cggaggaagg ccattcggct cccgcttatt acgattactt cgagtatcgt cccgagagcg
    11941 gttga
//
