LOCUS       NC_000964               3779 bp    DNA     linear   CON 18-SEP-2018
DEFINITION  Bacillus subtilis subsp. subtilis str. 168 complete genome.
ACCESSION   NC_000964
VERSION     NC_000964.3
DBLINK      BioProject: PRJNA76
            BioSample: SAMEA3138188
KEYWORDS    RefSeq; complete genome.
SOURCE      Bacillus subtilis subsp. subtilis str. 168
  ORGANISM  Bacillus subtilis subsp. subtilis str. 168
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1
  AUTHORS   Borriss,R., Danchin,A., Harwood,C.R., Medigue,C., Rocha,E.P.C.,
            Sekowska,A. and Vallenet,D.
  TITLE     Bacillus subtilis, the model Gram-positive bacterium: 20 years of
            annotation refinement
  JOURNAL   Microb Biotechnol 11 (1), 3-17 (2018)
   PUBMED   29280348
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Belda,E., Sekowska,A., Le Fevre,F., Morgat,A., Mornico,D.,
            Ouzounis,C., Vallenet,D., Medigue,C. and Danchin,A.
  TITLE     An updated metabolic view of the Bacillus subtilis 168 genome
  JOURNAL   Microbiology (Reading, Engl.) 159 (Pt 4), 757-770 (2013)
   PUBMED   23429746
REFERENCE   3  (bases 1 to 3779)
  AUTHORS   Barbe,V., Cruveiller,S., Kunst,F., Lenoble,P., Meurice,G.,
            Sekowska,A., Vallenet,D., Wang,T., Moszer,I., Medigue,C. and
            Danchin,A.
  TITLE     From a consortium sequence to a unified sequence: the Bacillus
            subtilis 168 reference genome a decade later
  JOURNAL   Microbiology (Reading, Engl.) 155 (Pt 6), 1758-1775 (2009)
   PUBMED   19383706
REFERENCE   4  (bases 1 to 3779)
  AUTHORS   Kunst,F., Ogasawara,N., Moszer,I., Albertini,A.M., Alloni,G.,
            Azevedo,V., Bertero,M.G., Bessieres,P., Bolotin,A., Borchert,S.,
            Borriss,R., Boursier,L., Brans,A., Braun,M., Brignell,S.C., Bron,S.,
            Brouillet,S., Bruschi,C.V., Caldwell,B., Capuano,V., Carter,N.M.,
            Choi,S.K., Cordani,J.J., Connerton,I.F., Cummings,N.J., Daniel,R.A.,
            Denziot,F., Devine,K.M., Dusterhoft,A., Ehrlich,S.D., Emmerson,P.T.,
            Entian,K.D., Errington,J., Fabret,C., Ferrari,E., Foulger,D.,
            Fritz,C., Fujita,M., Fujita,Y., Fuma,S., Galizzi,A., Galleron,N.,
            Ghim,S.Y., Glaser,P., Goffeau,A., Golightly,E.J., Grandi,G.,
            Guiseppi,G., Guy,B.J., Haga,K., Haiech,J., Harwood,C.R., Henaut,A.,
            Hilbert,H., Holsappel,S., Hosono,S., Hullo,M.F., Itaya,M., Jones,L.,
            Joris,B., Karamata,D., Kasahara,Y., Klaerr-Blanchard,M., Klein,C.,
            Kobayashi,Y., Koetter,P., Koningstein,G., Krogh,S., Kumano,M.,
            Kurita,K., Lapidus,A., Lardinois,S., Lauber,J., Lazarevic,V.,
            Lee,S.M., Levine,A., Liu,H., Masuda,S., Mauel,C., Medigue,C.,
            Medina,N., Mellado,R.P., Mizuno,M., Moestl,D., Nakai,S., Noback,M.,
            Noone,D., O'Reilly,M., Ogawa,K., Ogiwara,A., Oudega,B., Park,S.H.,
            Parro,V., Pohl,T.M., Portelle,D., Porwollik,S., Prescott,A.M.,
            Presecan,E., Pujic,P., Purnelle,B., Rapoport,G., Rey,M.,
            Reynolds,S., Rieger,M., Rivolta,C., Rocha,E., Roche,B., Rose,M.,
            Sadaie,Y., Sato,T., Scanlan,E., Schleich,S., Schroeter,R.,
            Scoffone,F., Sekiguchi,J., Sekowska,A., Seror,S.J., Serror,P.,
            Shin,B.S., Soldo,B., Sorokin,A., Tacconi,E., Takagi,T.,
            Takahashi,H., Takemaru,K., Takeuchi,M., Tamakoshi,A., Tanaka,T.,
            Terpstra,P., Togoni,A., Tosato,V., Uchiyama,S., Vandebol,M.,
            Vannier,F., Vassarotti,A., Viari,A., Wambutt,R., Wedler,H.,
            Weitzenegger,T., Winters,P., Wipat,A., Yamamoto,H., Yamane,K.,
            Yasumoto,K., Yata,K., Yoshida,K., Yoshikawa,H.F., Zumstein,E.,
            Yoshikawa,H. and Danchin,A.
  TITLE     The complete genome sequence of the gram-positive bacterium Bacillus
            subtilis
  JOURNAL   Nature 390 (6657), 249-256 (1997)
   PUBMED   9384377
REFERENCE   5  (bases 1 to 3779)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2018) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   6  (bases 1 to 3779)
  AUTHORS   Genoscope -,C.E.A.
  CONSRTM   AMAbiotics and Genoscope
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2013) A. Danchin, AMAbiotics, Bldg G1, 2, rue
            Gaston Cremieux, 91000 EVRY antoine.danchin@normalesup.org Bldg G1
            Genoscope / CEA (Commissariat a l'energie atomique et aux energies
            alternatives): BP 191 91006 EVRY cedex - FRANCE (E-mail:
            seqref@genoscope.cns.fr - Web: www.genoscope.cns.fr)
REFERENCE   7  (bases 1 to 3779)
  AUTHORS   Genoscope -,C.E.A.
  CONSRTM   Institut Pasteur and Genoscope
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2009) Genoscope - Centre National de Sequencage :
            BP 191 91006 EVRY cedex - FRANCE (E-mail : seqref@genoscope.cns.fr -
            Web : www.genoscope.cns.fr)
REFERENCE   8  (bases 1 to 3779)
  AUTHORS   Kunst,F., Ogasawara,N., Yoshikawa,H. and Danchin,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-1997) I. Moszer, A. Danchin, Genetique des Genomes
            Bacteriens, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris
            Cedex 15, FRANCE. E-mail: moszer@pasteur.fr,
            antoine.danchin@normalesup.org Phone: +33 (0)1 45 68 84 41, Fax: +33
            (0)1 45 68 89 48
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AL009126.
            On Aug 12, 2009 this sequence version replaced NC_000964.2.
            On or before Mar 17, 2009 this sequence version replaced
            gi:32468687, gi:32468690, gi:32468695, gi:32468704, gi:32468715,
            gi:32468723, gi:32468738, gi:32468743, gi:32468745, gi:32468758,
            gi:32468767, gi:32468778, gi:32468787, gi:32468796, gi:32468802,
            gi:32468809, gi:32468813, gi:32468825, gi:32468830, gi:32468833,
            gi:32468840, gi:38680335.
            Annotation results relative to BLAST similarities, COG
            assignations, enzymatic function prediction (PRIAM software), TMHMM
            and SignalP predictions, and synteny conservation (Syntonizer
            software) are available in the MicroScope annotation platform
            http://www.genoscope.cns.fr/agc/microscope.
            The large cluster comprising the i, h and g rDNA regions could not
            be properly resolved, we therefore incorporated the original
            sequence of rRNAihg in the final assembly.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Bacillus subtilis subsp. subtilis str. 168"
                     /mol_type="genomic DNA"
                     /strain="168"
                     /sub_species="subtilis"
                     /type_material="type strain of Bacillus subtilis"
                     /db_xref="taxon:224308"
     gene            complement(1..933)
                     /gene="hprK"
                     /locus_tag="BSU_35000"
                     /old_locus_tag="BSU35000"
     CDS             complement(1..933)
                     /gene="hprK"
                     /locus_tag="BSU_35000"
                     /old_locus_tag="BSU35000"
                     /EC_number="2.7.4.-"
                     /EC_number="2.7.11.-"
                     /function="16.3: Control"
                     /experiment="publication(s) with functional evidences,
                     PMID:10636874, 12009882, 12359875, 12411438, 12779331,
                     15023355, 16267306, 17878158, 20487301, 21992469, 27226549"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 10636874, 12009882, 12359875,
                     12411438, 12779331, 15023355, 16267306, 17878158, 20487301,
                     21992469, 27226549; Product type e : enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase/phosphorylase
                     (pyrophosphate-producing)"
                     /protein_id="NP_391380.1"
                     /db_xref="EnsemblGenomes-Gn:BSU35000"
                     /db_xref="EnsemblGenomes-Tr:CAB15505"
                     /db_xref="GOA:O34483"
                     /db_xref="InterPro:IPR003755"
                     /db_xref="InterPro:IPR011104"
                     /db_xref="InterPro:IPR011126"
                     /db_xref="InterPro:IPR028979"
                     /db_xref="SubtiList:BG14125"
                     /db_xref="UniProtKB/Swiss-Prot:O34483"
                     /translation="MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQEE"
     gene            1115..2305
                     /gene="nagA"
                     /locus_tag="BSU_35010"
                     /old_locus_tag="BSU35010"
     CDS             1115..2305
                     /gene="nagA"
                     /locus_tag="BSU_35010"
                     /old_locus_tag="BSU35010"
                     /EC_number="3.5.1.25"
                     /function="16.2: Construct biomass (Anabolism)"
                     /function="16.13: Shape"
                     /function="16.11: Scavenge (Catabolism)"
                     /experiment="publication(s) with functional evidences,
                     PMID:6774970, 14557261, 21602348"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 6774970, 14557261, 21602348;
                     Product type e: enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="CE9"
                     /protein_id="NP_391381.1"
                     /db_xref="EnsemblGenomes-Gn:BSU35010"
                     /db_xref="EnsemblGenomes-Tr:CAB15506"
                     /db_xref="GOA:O34450"
                     /db_xref="InterPro:IPR003764"
                     /db_xref="InterPro:IPR006680"
                     /db_xref="InterPro:IPR011059"
                     /db_xref="InterPro:IPR032466"
                     /db_xref="PDB:2VHL"
                     /db_xref="SubtiList:BG12630"
                     /db_xref="UniProtKB/Swiss-Prot:O34450"
                     /translation="MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKEPYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDASFSTLDIMSSRLPEEGTTSFLATTITQEH
                     GNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVELFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRHLKDESIIASMGHTDADSALLSDAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSENAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     gene            2302..3030
                     /gene="nagBA"
                     /locus_tag="BSU_35020"
                     /old_locus_tag="BSU35020"
     CDS             2302..3030
                     /gene="nagBA"
                     /locus_tag="BSU_35020"
                     /old_locus_tag="BSU35020"
                     /EC_number="3.5.99.6"
                     /function="16.2: Construct biomass (Anabolism)"
                     /function="16.11: Scavenge (Catabolism)"
                     /experiment="publication(s) with functional evidences,
                     PMID:15755726, 18436239"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 15755726, 18436239; Product
                     type e: enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="NP_391382.1"
                     /db_xref="EnsemblGenomes-Gn:BSU35020"
                     /db_xref="EnsemblGenomes-Tr:CAB15507"
                     /db_xref="GOA:O35000"
                     /db_xref="InterPro:IPR004547"
                     /db_xref="InterPro:IPR006148"
                     /db_xref="InterPro:IPR018321"
                     /db_xref="PDB:2BKV"
                     /db_xref="PDB:2BKX"
                     /db_xref="SubtiList:BG12631"
                     /db_xref="UniProtKB/Swiss-Prot:O35000"
                     /translation="MKVMECQTYEELSQIAARITADTIKEKPDAVLGLATGGTPEGTYR
                     QLIRLHQTENLSFQNITTVNLDEYAGLSSDDPNSYHFYMNDRFFQHIDSKPSRHFIPNG
                     NADDLEAECRRYEQLVDSLGDTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQ
                     ANARYFPSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASA
                     LHLHSDVTVLIDREAASLRP"
     gene            3048..3779
                     /gene="nagR"
                     /locus_tag="BSU_35030"
                     /old_locus_tag="BSU35030"
     CDS             3048..3779
                     /gene="nagR"
                     /locus_tag="BSU_35030"
                     /old_locus_tag="BSU35030"
                     /experiment="publication(s) with functional evidences,
                     PMID:16207374, 20047956, 21602348, 23667565, 24673833,
                     26162881"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 16207374, 20047956, 21602348,
                     23667565, 24673833, 26162881; Product type r : regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|GntR,DBD-SUPERFAMILY|0037767"
                     /protein_id="NP_391383.1"
                     /db_xref="EnsemblGenomes-Gn:BSU35030"
                     /db_xref="EnsemblGenomes-Tr:CAB15508"
                     /db_xref="GOA:O34817"
                     /db_xref="InterPro:IPR000524"
                     /db_xref="InterPro:IPR011663"
                     /db_xref="InterPro:IPR028978"
                     /db_xref="InterPro:IPR036388"
                     /db_xref="InterPro:IPR036390"
                     /db_xref="PDB:2WV0"
                     /db_xref="PDB:4U0V"
                     /db_xref="PDB:4U0W"
                     /db_xref="PDB:4U0Y"
                     /db_xref="PDB:4WWC"
                     /db_xref="SubtiList:BG14124"
                     /db_xref="UniProtKB/Swiss-Prot:O34817"
                     /translation="MNINKQSPIPIYYQIMEQLKTQIKNGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGLLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYDHIERYNSIPISRAKQELEPSAATTEEANILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
ORIGIN
        1 ctattcttct tgttcaccgt cttcaatgac gtccgccagc ttgttggtaa attgttcagc
       61 ggcattcagc cccatccgct tcaatctgaa gttcattgct gccacttcga tgatgactgc
      121 aagatttcgg cctgggcgga cgggaatcgt cagttttgta atttctgtat caatgatttt
      181 catggtctct tcctcaagtc cgagccggtc gtattgcttg ccctgctccc agagttcaag
      241 attcatgaca atcgtgattc gtttattgct tctgaccgcg ccagcgccaa atagcgtcat
      301 cacattgata atgccaaggc ccctgatctc aagaaggtgc tcaattaatt ccggagcgtt
      361 tcccacaaga gtatcctgat cctcctgccg tatttcaacg caatcatcgg caacaaggcg
      421 atgccctctt ttcacaagct ctagcgctgt ttcgcttttt ccgacgccgc tttttcctgt
      481 gatcagcacg ccgacaccat atatatcgac aagaacgcca tgaattgctg tggtaggcgc
      541 cagcctgctc tcaaggaagt tggttaaacg gcttgacagt cttgtcgttt tcagcggcga
      601 tctgaggaca ggcaccccat ttttctcgga ggcgtcaatc agctcctgcg ggatgggcat
      661 atctctagaa agaataatag ctggtgttac atcagtgcac agagaatcca ttcgctgctt
      721 tttctcctct tcaggaagct gttcaaagaa agaaagctct gtttttccga gaagctgcac
      781 gcgctccctc gggtaatatg taaaatatcc ggcaatttca atacctggtc ttgataggtc
      841 actcattgta atcgggcggt taattccttc ttctccgctg attaattcca aattgaactg
      901 ttccattacg tcttttgtgc gaacctttgc cacgatatgt tcctcctgtt ccgggctgcc
      961 ccgagcttgc tcacaatact ttcattttat cactttcggg cttgaaccta aaacagattt
     1021 tataaaaggg gggaaaacac ctcagctggt ctagatcact agtctgaaaa agagtaaaat
     1081 aaaggtattc aaattccaga aaggcggatc atctatggca gagagtcttc ttatcaaaga
     1141 cattgcgatc gtgacagaaa atgaagtaat caaaaacggc tatgtcggaa tcaatgacgg
     1201 aaaaatcagc acagtttcaa cagaacggcc caaggagcca tattcaaaag agattcaagc
     1261 acctgcggat tccgttttgc ttcccggcat gattgacatt catatccacg gcggctatgg
     1321 cgcagataca atggatgcta gcttttccac gctcgacatc atgtcgtcaa gactgcccga
     1381 agaaggcacg acgagctttt tagccacaac gattacccaa gagcatggga acatttctca
     1441 agctttggtg aacgcaagag agtggaaagc ggctgaagag tcctctttat taggtgcaga
     1501 actgctcggc attcatttag agggcccctt tgtttcgcct aaaagagccg gagcgcagcc
     1561 aaaggagtgg attaggcctt cggatgttga gcttttcaaa aagtggcagc aggaggcggg
     1621 cggactgatc aaaattgtta cacttgcgcc ggaggaggat cagcattttg aactgatccg
     1681 ccatttgaaa gacgaatcga ttatcgcatc catggggcac acggatgccg attccgcatt
     1741 gctgtcggat gccgcaaaag caggggcgag ccatatgact catctgtaca acgcgatgag
     1801 cccgtttcat caccgagagc ccggagtgat cggaacggcg ctggcccatg atgggtttgt
     1861 gacagagctt atcgccgatg gcattcactc ccatccttta gctgcaaagc ttgctttttt
     1921 ggcgaaaggc agcagcaagc tcattttaat taccgattcc atgagggcaa aaggactgaa
     1981 agacggcgta tatgagttcg gcggccaaag tgtgacggta agaggacgaa cagcacttct
     2041 ttcagacgga acccttgccg gttcgatcct caaaatgaac gaaggcgcac ggcatatgcg
     2101 tgagtttaca aattgctctt ggacggatat agctaatata acttctgaaa acgcggccaa
     2161 acagctgggc atctttgacc gaaaaggcag tgtgactgtg ggaaaagatg cagatttggt
     2221 gattgtcagc agtgattgcg aggtgattct caccatttgc cgcggaaaca ttgcatttat
     2281 atccaaggag gctgaccaga tatgaaagta atggaatgtc aaacgtatga agagctaagc
     2341 caaatagcag ccagaataac ggcagataca atcaaagaaa aacctgatgc tgttctcggt
     2401 ttagcgacgg gcggcacacc ggaaggcacg tatcgccagc tgatccgcct gcaccaaact
     2461 gagaatctct catttcaaaa catcactaca gttaatttgg atgagtacgc cggactttca
     2521 agcgatgacc cgaacagcta tcacttctat atgaacgacc gtttttttca gcatatcgac
     2581 agcaagccta gccggcattt tattccgaat ggaaatgcag acgatttgga ggccgaatgc
     2641 agacggtatg aacagctagt cgattccctc ggcgacactg acattcagct tctcggcatc
     2701 ggccgaaacg gacacatcgg ctttaacgaa ccgggaacgt ctttcaagtc gcgaactcat
     2761 gttgtgacat taaatgagca aacccgccaa gcgaatgcca gatattttcc ctccatagac
     2821 agcgtgccga aaaaagcact gacaatggga attcaaacga tactctcaag caagcgcatt
     2881 ctgctgctta tttctggaaa aagcaaagcg gaagctgttc gtaagctgtt agaaggaaac
     2941 ataagcgaag attttcccgc atctgctttg cacctgcatt ccgatgttac ggttttgatt
     3001 gatcgtgaag ccgcgtcatt aagaccttga aaggaacatg ctgacttatg aatatcaata
     3061 aacaatcgcc tattccgatt tactatcaga ttatggagca attaaaaacc caaattaaga
     3121 acggagagct gcagccggat atgcctcttc cttctgagcg cgaatatgcc gaacaattcg
     3181 ggatcagccg gatgacagtt cgccaggcgc tttctaattt agttaatgaa ggcttgctct
     3241 atcgcctgaa agggcggggc acctttgtca gcaagccaaa aatggaacaa gcacttcaag
     3301 ggctgacaag ctttaccgag gatatgaaaa gccgcgggat gacaccgggc agcaggctca
     3361 ttgattatca gcttattgat tcaactgagg agctcgcggc tatattaggc tgcgggcacc
     3421 cctcctctat ccataaaatc actcgggtgc ggctggcaaa tgatattccg atggcgattg
     3481 agtcctcaca tattccgttt gagcttgcgg gtgaattgaa cgaatcgcat tttcagtcgt
     3541 cgatctatga tcatattgaa aggtacaaca gcataccgat ttcccgtgca aaacaggagc
     3601 ttgagccaag cgctgccacc acggaagaag cgaatattct tggtattcaa aagggagcgc
     3661 ctgtcctatt aattaaacga acaacatatc tgcagaacgg aactgctttt gagcatgcaa
     3721 aatccgtata cagaggcgac cgttatacat ttgtccacta tatggatcgt ctttcataa
//
