LOCUS       CP001834               15446 bp    DNA     linear   BCT 30-JAN-2014
DEFINITION  Lactococcus lactis subsp. lactis KF147, complete genome.
ACCESSION   CP001834
VERSION     CP001834.1
DBLINK      BioProject: PRJNA41115
            BioSample: SAMN02603087
KEYWORDS    .
SOURCE      Lactococcus lactis subsp. lactis KF147
  ORGANISM  Lactococcus lactis subsp. lactis KF147
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 15446)
  AUTHORS   Siezen,R.J., Starrenburg,M.J., Boekhorst,J., Renckens,B.,
            Molenaar,D. and van Hylckama Vlieg,J.E.
  TITLE     Genome-scale genotype-phenotype matching of two Lactococcus lactis
            isolates from plants identifies mechanisms of adaptation to the
            plant niche
  JOURNAL   Appl. Environ. Microbiol. 74 (2), 424-436 (2008)
   PUBMED   18039825
REFERENCE   2  (bases 1 to 15446)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B., Wels,M., van Hijum,S.A.,
            Molenaar,D. and van Hylckama Vlieg,J.E.
  TITLE     Complete genome sequence of Lactococcus lactis subsp. lactis KF147,
            a plant-associated lactic acid bacterium
  JOURNAL   J. Bacteriol. 192 (10), 2649-2650 (2010)
   PUBMED   20348266
REFERENCE   3  (bases 1 to 15446)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
            Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
  TITLE     Complete genome sequence of Lactococcus lactis ssp lactis KF147
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 15446)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
            Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) NIZO Food Research, Kluyver Centre for
            Genomics of Industrial Fermentation, PO Box 20, Ede 6710 BA, The
            Netherlands
COMMENT     On or before Dec 18, 2009 this sequence version replaced
            EU255902.1, EU255903.1, EU255904.1, EU255905.1, EU255906.1,
            EU255907.1, EU255908.1, EU255909.1, EU255910.1, EU255911.1,
            EU255912.1, EU255913.1, EU255914.1, EU255915.1, EU255916.1,
            EU255917.1, EU255918.1.
            Bacteria source: Dr. W.J. Kelly, Agresearch Limited, New Zealand;
            william.kelly@agresearch.co.nz.
FEATURES             Location/Qualifiers
     source          1..15446
                     /organism="Lactococcus lactis subsp. lactis KF147"
                     /mol_type="genomic DNA"
                     /strain="KF147"
                     /isolation_source="sprouts"
                     /host="mung bean"
                     /sub_species="lactis"
                     /db_xref="taxon:684738"
     gene            complement(1..1446)
                     /gene="sucP"
                     /locus_tag="LLKF_2259"
     CDS             complement(1..1446)
                     /gene="sucP"
                     /locus_tag="LLKF_2259"
                     /EC_number="2.4.1.7"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13|GH13_18"
                     /protein_id="ADA65829.1"
                     /translation="MPIQNKVMLITYADSLGKNLKDLQKILAEDLEGAVGGVHLLPFFP
                     STGDRGFAPQDYRQVDSAFGDWADIEAIGERYYLMFDFMINHLSRQSPEFIDFKAKKED
                     SDFANLFLRNQNFWPENRPTQADIDLIYKRKDKAPFQEVTFADGTTELVWNTFGEEQID
                     LDVNQAVTRRFIKETLEQLIDHGCDIVRLDAFAYAIKKLDTNDFFVEPEIWDLLNDVRD
                     ITIAKGCEILPEIHEHYSIQHKIADHNFYIYDFALPMVSLYTLYSGQTKQLAKWLKMSP
                     MKQFTTLDTHDGIGVVDAKDILTDEETLYTSEQLYKVGANVKKIFSSEAYNNLDIYQIN
                     STYYSALGDNDDAYLLARAFQVFAPGIPQVYYVGLLAGKNDLDLLESSKEGRNINRHYY
                     DVEEVKTELKRPVVQKLLNLLTFRNTAQAFDLDGGIEIVTPSDNEIVITRFDAAKENVA
                     VLTANAKTYAFEITLNDEKITLL"
     gene            complement(1533..2360)
                     /locus_tag="LLKF_2260"
     CDS             complement(1533..2360)
                     /locus_tag="LLKF_2260"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00751|3.A.1.1.28"
                     /protein_id="ADA65830.1"
                     /translation="MKNKENKLPYLLLLIVGAILILVPIYLTVVSSFKDTPQIMQHFFS
                     LPKPFTMENYTRLIEDGIGKYFLNSVIISVVSIIMIILVVPMAAFSIARNIGRKTAFTI
                     MYSLLILGIFVPFQVIMLPITNQMTAMGLMNLPGLMLLYLTYAIPQTLFLYVGYIKLSI
                     PESLDEAAEIDGANKMTMFIKVAFPLMKPMHATTLILNALWIWNDFLLPLLILNRDDSF
                     WTLPLFQYNYQGQYMSDFGPSFASYIIGIVTITIVYLFFQKRIISGMSNGAVK"
     gene            complement(2373..3242)
                     /locus_tag="LLKF_2261"
     CDS             complement(2373..3242)
                     /locus_tag="LLKF_2261"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00750|3.A.1.1.28"
                     /protein_id="ADA65831.1"
                     /translation="MKDKFINKYWGYLFLLIPIILQVIFFYLPMIRGVIFSFTNWTGLT
                     DNYDYIGFKNYTAIFSDPKFIKTIKFTIIFTIGMILGQVSIGILVARALNAKLRTSNFF
                     RGVFFFPAVIATVTIGMIFKQIFAYGIPLIGETFHIDFLKENLLSNPKTVVYAVLFVAL
                     WQGVATPTIIFLAGLQSIPQEILEAAAIDGASKRQIFKSIEIPFLIPSISMVFILALKG
                     GLTAFDNIFVLTGGGPNDMTATLGMLVYNTAFKNNSFGYANAIAVVLFIIIVVISLIQQ
                     AVSKKFEV"
     gene            complement(3343..4587)
                     /locus_tag="LLKF_2262"
     CDS             complement(3343..4587)
                     /locus_tag="LLKF_2262"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00749|3.A.1.1.28"
                     /protein_id="ADA65832.1"
                     /translation="MKKRTKLTLSLLSLASVALLASCGSSASKKTEIEFFTQKKETLAI
                     TKEIAKDFEKENKDIKVKVVSVPDPGNVLKTRISSKDTPDIVNVFPQNADFKEWAANNV
                     FEDLTGKDFMSNIKDGVAEQYAIKDKVYSVPLDSNAWGFFYNADAFKKLGLEAPKTWDE
                     FETLVSDIKSKSKDQAPFALSLTQGDAWSLNGFGQLAWAEADGGFDGAQNALRFSPKGA
                     IKTSNPDFQAVAKQLDILKGNGQKGADGATYDDAIAAFATGKSLILPQGIWALSAVQQQ
                     KPNFEIRSFAYPGKTEADAMSVGAADMALSISSTSKNKAAAEKFVKYFSTAKVFQKYYD
                     VNGEPTAVKGVQTEGKTPEIEGITRLTFTDKQFVWLQSKWDSEADFHTLTANYARTGNA
                     DAMAKELNVFFDAMK"
     gene            4737..5606
                     /locus_tag="LLKF_2263"
     CDS             4737..5606
                     /locus_tag="LLKF_2263"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0035607,DBD-SUPERFAMILY|0035607"
                     /protein_id="ABX75749.1"
                     /translation="MEYKEFHQNFLDINLDFVGNEATIPNFSFGPAIRENYVIHYITSG
                     SGRYMIYGFEHQLKAGDCFIIPADVETFYQSDALTPWAYYWLGLSGHVVNDLFARTALD
                     DKGWILENVSKTEFIEHFSKIQNLISDDDKTVDLDIQVELFALMKSLITLFPKSITEHK
                     NQSDYYAEKAYTFINQNYSQSIKIKDVLAHVMISRAYLFTIFKHKYGLSPQKYLIDLRM
                     AKAAMLLIHSDNLVSQISEAVGFSDSLSFSSAFKKRYGVSPTKFKTQKHDNLMLETLNN
                     MNLNRLKK"
     gene            complement(5678..7222)
                     /gene="purH"
                     /locus_tag="LLKF_2264"
     CDS             complement(5678..7222)
                     /gene="purH"
                     /locus_tag="LLKF_2264"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /note="bifunctional; purH2"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="ABX75750.1"
                     /translation="MTKRALISVSDKTGIVAFAEKLRELGWEIISTGGTKTALDAAGVA
                     TIAIDDVTGFPEMMDGRVKTLHPKIHGGLLARRDLDTHVSAMKDHEILPIDLVVVNLYP
                     FKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPTDYELVLTEFAENGDT
                     TYETRQKLAAKVFRHTAAYDALIAEYFTAQVGEVKPEKLTLTYDLKQPMRYGENPQQNA
                     DFYESALPIDYSISQAKQLNGKELSFNNIRDADAAIRIIRDFTLPTVVALKHMNPCGIG
                     TAETIETAWDYAYDADPVSIFGGIVVLNREVDAATAEKMNPIFLEIIIAPSYSDDALAI
                     LTNKKKNLRILVLNFAQQKASETPKEVTGVVGGLLVQDQDTTVELQESWEIVTKRHPTA
                     VEVEALEFAWRSVKYVKSNGIIITNHHQTLGVGPGQTNRVASVKIAIEQASDRLEGAVL
                     ASDAFFSFADNVEEIAKAGIKAIIQPGGSVRDQDAIDKADKHGIAMVFTGARHFRH"
     gene            complement(7347..8831)
                     /gene="galT"
                     /locus_tag="LLKF_2265"
     CDS             complement(7347..8831)
                     /gene="galT"
                     /locus_tag="LLKF_2265"
                     /EC_number="2.7.7.10"
                     /note="galT2"
                     /codon_start=1
                     /transl_table=11
                     /product="Galactose 1-phosphate uridylyltransferase"
                     /protein_id="ABX75751.1"
                     /translation="MNISQAVIDFADLAIKLGAYEVADRIYLQNLILALIGEDDLAENL
                     QPSGNDDSLTLSDFLCAVAKANGQFDEIAANEDIFQAKLMNFLTPTPAHVNRRFKEKYA
                     QSPKAATDYFFDLSKKNDYIKTRAIAKNEHFYSASPYGELEITINLSKPEKDPKQIAAE
                     KNAVQSSYPKCLLCIENEGYAGRLNHPARTNHRIVQLDLAGETWGFQYSPYAYFNEHSI
                     ILAEKHSPMKISQVTFAHLLAIVDSLPTYFAGSNADLPIVGGSILSHDHYQAGRHDFPM
                     AKAELKGQFIIPTFPTVGAGIVNWPMSVIRLTADNRQDLVAASTFILEKWQQYTDETVE
                     IVARTADGTPHHTITPIARRRGADFEIDLVLRDNNTSEQHPDGIFHPHSDVQHIKRENI
                     GLIEVMGLAILPGRLKTELEEVKRYILGEADTVAAYHQPWAEALKQGYQNEAIDTYMQA
                     ALGHVFLTVLEHAGVFKDTLDGNAAFARFIAELGAV"
     gene            complement(8828..10018)
                     /gene="galK"
                     /locus_tag="LLKF_2266"
     CDS             complement(8828..10018)
                     /gene="galK"
                     /locus_tag="LLKF_2266"
                     /EC_number="2.7.1.6"
                     /note="galK2"
                     /codon_start=1
                     /transl_table=11
                     /product="Galactokinase"
                     /protein_id="ABX75752.1"
                     /translation="MRNKYVNTSNETRSHRKFIALFGDGAKANYFSPGQINLIGEHTDY
                     NGGYVFPAAISIGTFGVARKRTDSLVKVYSLNFETVGVIAFSLENELVNTPEANWGNFV
                     KGYLLKLKEAGHAIQTGFELVIEGTIPNGSGLSSSASLELLVGTVVNDLYHLGLDKLTL
                     VQLGQKVENEFIGVNSGIMDQFAIGFGEIGKAILLDTNTLKYELVPAEFGDYKIVIMNT
                     NKRRELADSKYNERRAECEEALKRLQTKLEITALGQLTPTEFEENRALIGDVTLEKRAK
                     HAVYENERTKLAKTCLEAGELQAFGQLLNASHESLKNDYEVTGIELDTLAETAQGLSGV
                     LGARMTGAGFGGCGIALVATSEIETFITKLSATYLEKIGYAASFYVATIADGARKL"
     gene            complement(10015..12231)
                     /gene="aga"
                     /locus_tag="LLKF_2267"
     CDS             complement(10015..12231)
                     /gene="aga"
                     /locus_tag="LLKF_2267"
                     /EC_number="3.2.1.22"
                     /codon_start=1
                     /transl_table=11
                     /product="GH36"
                     /protein_id="ABX75753.1"
                     /translation="MTLITFDESKKIFHLSNTAISYLIGIEKESYLSHLYFGKAIKTYH
                     GGRKYPAMNRSFSPNPDGMPLKTRDFSLDVISQEFPSYGHGDFRNPAVQIKQANGSFIT
                     EFVYDSYEIISGKPILDGLPATYVEADEEAETLVITLVDKLLNLKLKLSYTIYAQRDVI
                     ARNTLLENHGVAPVVIEKLASISVDLPEQDLELISLPGRHVKEREIDRQPIQRGTRIID
                     SKRGSSSHQANPFIAIVAPKTDELTGTAIGLTLVYSGNHEMLVERDQFSQTRVMAGINP
                     FGFEWELGIDASFQSPEALLVYSDQGLNGMSQTFHDLLQNRLARGQYRQAERPILINNW
                     EATYFDFDTDKIKKIVDSAADLGIELFVLDDGWFGKRDDDISGLGDWFENTEKLKGGLK
                     AIADYVHQKKMSFGLWFEPEMVNADSDLFRQHPDYALQTPDRSMSTSRDQYVLDFSRKE
                     VRETITRQMRAILDTIDIDYIKWDMNRNLTEVYSVTASAAHQGEVFHRYVLGLYEMLEE
                     LTTDYDHVLWEGCSGGGGRFDAGFLHYMPQSWTSDNTDAIERLEIQYGTSLVYPISSMG
                     AHVSAVPNHQTHRETGLEIRGDVAMSGVFGYELNLQDMTQAEKEVVLEQVTFYKTHRKL
                     LQYGKFHRLLSPFESDQTAWLFVNGDQSQAIGFYFRKYAESAGPLRTLKFTGLAPEKTY
                     QVNGDAIYGGDELMSVGLYIYPFLDGDYQSRKFVINEVKSNEIK"
     gene            complement(12362..13399)
                     /gene="galR"
                     /locus_tag="LLKF_2268"
     CDS             complement(12362..13399)
                     /gene="galR"
                     /locus_tag="LLKF_2268"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0044558"
                     /protein_id="ABX75754.2"
                     /translation="MASIREIAKLAGVSPATVSRVLNADETMSVSPATRTRIIKVANQL
                     NYHKVENLGPKSPKQSNKLSIAVIKTHSSKRENDDPYFRLIQEGIALEAGNWNFRLETL
                     KLGEVSLEQLAQFGAVLTIGAFTDETLADIYKVNQNLIVVDNHFASSRYDLVHTDFAKQ
                     TEQVLDYLYEQGHRQIAFIGGEIRTVDLKGQKQHLLADVRTTAYENWMTIHGLSDKIQL
                     KTGDWSMGFALNATNALIEASGDQLPTAIISASDPMSIGIYRALQLKNITIPEAISVFS
                     FDDIEMVGYLSPPLSTVHIDSLEIGRVAVRLAKERISDGRKTALRVEVASEIIVRESVR
                     KNKLS"
     gene            complement(13524..15446)
                     /gene="fbp"
                     /locus_tag="LLKF_2269"
     CDS             complement(13524..15446)
                     /gene="fbp"
                     /locus_tag="LLKF_2269"
                     /EC_number="3.1.3.11"
                     /note="fbp2"
                     /codon_start=1
                     /transl_table=11
                     /product="Fructose 1,6-bisphosphatase"
                     /protein_id="ABX75755.1"
                     /translation="MQENKYLSLLAEKYETKDKVASELINLEAILNLPKGTELYISDIH
                     AEYKAFNHIIRTGAGNIKEKIDAIFGDEDPEEMDRLAMLIAYPTDMLQRKPKDLEAAND
                     WSSQTIHRLIKLLRFLGTKYSRSKVRKAVHEDFRYFTEELMLSLETNQSEKADYYQQII
                     KRLIELNQADKFIRSLCMTIQQLTIDHLHVVGDVFDRGAGADKVMDRLIQFHNVDFQWG
                     NHDVLWMGAYAGNMASLATLLRIAVKYNYLYELETAYSLNLRPLFLFAEEHYELNSAFK
                     PTARPDDDLLGVENQARLTKVHQALTVIQFKLEGQIIARRPEFEMGDRNLLEKIDYKHK
                     TITLNDTDYPLENTCFQTVSQYHPNELTESEAYVMAAILSSLQKSEKFQRHMQFLVEKG
                     SAYLVYNRHLLYHGCIPLKKDGTFLSMSIGGTLYSGRPLLDKFEEHIRRGIRHTEIYDD
                     YSTDLMWYAWAGKCSPLFSRSSMTTFERYFISDKTTHKEGENPYFAYREKPEIMDMILA
                     EFGLNDPQSVVINGHTPVKVASGESPIKANGKLMVIDGGMSKAYQKTTGIAGYSLLNDS
                     YGFKIVTHAPFTSIEEILAKGTGNESLTNFLEEKPKRRLIKDTTIGDNLRADIMDLTAL
                     MAYMP"
ORIGIN
        1 tcataaaagt gtgatttttt cgtcattgag agtgatctcg aaagcgtaag ttttagcatt
       61 ggcggttaag acggcaacat tttctttggc tgcatcaaag cgcgtaatca caatctcatt
      121 gtcactcggt gtcacaattt caatcccgcc atcaaggtca aaagcctgcg ctgtgttacg
      181 gaatgtcaac agattcagta atttttgaac gaccggacgt ttgagttctg ttttaacttc
      241 ctcgacatca taataatggc ggttaatatt acggccttcc tttgaagact caagtaagtc
      301 tagatcgttt ttccctgcta ataggccaac gtagtacact tgaggaatgc caggtgcaaa
      361 gacttggaaa gcacgtgcta acaagtaagc atcatcattg tcacctaaag ctgaatagta
      421 agtcgaatta atttgataaa tatccagatt attataggct tccgatgaga atattttctt
      481 gacgttagca ccgaccttat agagttgctc agaagtatac agggtttctt catcagtaag
      541 aatatcctta gcatcaacaa caccaatccc atcatgggta tcaagggttg taaactgctt
      601 cataggagac atctttaacc atttggccaa ttgcttggtt tgaccagagt agagtgtata
      661 aagactaacc attggcaagg caaaatcata gatataaaaa ttatgatcag caattttatg
      721 ttggatgctg tagtgttcat ggatttctgg taggatttca cagcctttgg ctatcgtgat
      781 gtctctgaca tcgtttaaca agtcccagat ttctggctcc acaaagaaat cattggtgtc
      841 taatttctta atcgcataag caaaggcatc tagtcgaaca atgtcacacc catggtcaat
      901 caattgctct aaagtctctt tgatgaagcg acgtgtgacg gcttgattga catctaagtc
      961 aatctgctct tccccaaagg tgttccagac taattcagtc gtcccatccg caaaggtcac
     1021 ttcttgaaag ggtgctttgt ctttacgttt gtaaatgaga tcgatgtctg cctgggttgg
     1081 tcgattttct ggccaaaaat tttgattgcg taaaaagaga ttagcaaagt cagaatcctc
     1141 tttcttagcc ttgaaatcaa taaattctgg actctgacgc gacaaatgat tgatcataaa
     1201 gtcaaacatc aagtaataac gctcgccaat ggcttcgata tctgcccaat caccaaaagc
     1261 gctgtctact tgccgataat cttgcggtgc gaacccacgg tcacctgtgg atggaaagaa
     1321 tggcagtaaa tggacacctc ccactgcacc ttccaaatct tcggctagga ttttctggag
     1381 gtcctttaga tttttaccaa ggctatctgc gtaagtaatt aacatgactt tattttgaat
     1441 aggcatattt tctccttttg actttttgtc aatcgtttga taatcgtatt atatcaaaac
     1501 gcttacttga tgtaaacgtt ttcttttaag ttttatttca ccgcaccgtt actcatacct
     1561 gagatgatac gcttttggaa aaagagataa acaatagtaa tcgtcacgat accgatgata
     1621 taagaggcaa aacttggacc aaaatcactc atatattgcc cctgataatt gtactggaac
     1681 aaaggtaatg tccaaaatga atcgtctcgg ttaagaatga gcaatggcaa gagaaagtca
     1741 ttccaaatcc ataaagcatt gagaattaaa gtcgttgcgt gcatgggttt catcagggga
     1801 aaagcaactt tgataaacat agtcatctta ttagcaccat caatctctgc tgcttcatcc
     1861 aaactttcag gaattgataa cttaatataa ccgacataca agaacagggt ttgtggaatg
     1921 gcgtaagtca gatagaggag catcagacct ggcagattca tcaagcccat ggctgtcatc
     1981 tgattagtaa tcggcagcat gatcacttgg aaaggaacaa aaatgccaag aatcaaaagt
     2041 gaatacatga tggtaaaagc tgttttgcgg ccaatattac gtgcaatgga gaaagctgcc
     2101 attggcacga ctaaaataat catgatgatc gatacaacag agataataac tgaatttaag
     2161 aaatatttac caataccgtc ttcaatcaaa cgcgtatagt tttccatggt aaagggtttc
     2221 ggtaaactaa agaaatgttg cataatttgc ggtgtatctt taaatgagct gaccactgtt
     2281 aagtaaatgg gaaccaatat tagaatggca cctacaatga gtaacagtaa atacggtaat
     2341 ttattttctt tatttttcat tttcccacct ctttagactt caaacttctt agaaacagct
     2401 tgttgaatta atgaaatcac aacaatgatg ataaataaga ctactgcaat tgcattggca
     2461 tagccgaagc tattattttt gaaggctgtg ttatagacta acattcctaa tgtggcagtc
     2521 atgtcattag gcccaccacc tgtcagaaca aatatattat caaaagctgt caaaccacct
     2581 tttaaagcta aaataaagac catggagata gatgggatta agaaaggaat ttcaatactt
     2641 ttgaaaattt gtcttttgct tgccccatca atggctgctg cttccaaaat ctcttgcgga
     2701 atactttgca aaccagctag aaaaataatc gttggtgtcg ccacaccctg ccataaagcg
     2761 acaaataaaa cggcgtagac aactgtttta ggatttgaga gtagattttc tttcaagaag
     2821 tcaatgtgga acgtttcgcc aatcaaggga atgccgtaag caaaaatttg tttgaaaatc
     2881 ataccaattg taacggttgc aatcactgct ggaaagaaga acacgccacg aaagaaattt
     2941 gaggttctaa gtttggcatt cagggcgcgt gccactaaaa taccgatgct gacttgacct
     3001 aggatcatac caatcgtgaa aattatggta aacttaattg ttttaataaa ttttggatca
     3061 ctgaaaatag ctgtataatt tttaaaacca atataatcat aattgtccgt taagccagtc
     3121 caattggtaa aagagaagat aacgccacgg atcatcggca aataaaagaa aatcacttgc
     3181 agaataattg gaatcaagag gaagagatat ccccaatatt tattgataaa tttgtctttc
     3241 ataacgagaa ttccttttct aaatttgatc taatttatga actttagcct gaacgcctat
     3301 cgcgtaggtg ttcagaaaaa attcacaaat cagaaatcaa gcttatttca ttgcatcaaa
     3361 gaagacattg agctctttgg ccatggcatc agcattacca gtacgtgcat aatttgctgt
     3421 caaggtatgg aaatcagctt cactgtccca cttagattgt aaccaaacga attgtttatc
     3481 tgtaaaggtt aaacgtgtga ttccttcaat ttctggtgtt ttgccttctg tttgaacacc
     3541 tttgacagct gttggttcac cattgacatc gtagtatttt tggaaaactt tagccgttga
     3601 gaaatattta acaaattttt ctgctgctgc tttgttttta gacgtgctag aaattgaaag
     3661 tgccatatct gcagcaccaa cactcatcgc atcagcttct gttttacctg ggtaagcaaa
     3721 tgaccgaatt tcaaaatttg gcttttgttg ttggacagct gaaagtgccc aaatcccttg
     3781 tggcaagatt agtgatttac ctgttgcaaa agctgcaatc gcatcatcat aagtggcgcc
     3841 atcagcacct ttttgaccat tacctttgag aatatcaagt tgtttggcaa ctgcttgaaa
     3901 atctggattt gatgtcttaa tggcaccttt aggtgagaag cgcagcgcat tttgagcgcc
     3961 atcaaagcca ccatctgctt cagcccaagc caactgacca aaaccattaa gtgaccaggc
     4021 atctccctgt gttaaagata aagcaaacgg tgcttgatct ttacttttag acttgatatc
     4081 actgaccaat gtttcaaatt catcccaagt tttgggtgct tcaagcccta gtttcttaaa
     4141 ggcatcagca ttgtagaaga acccccaagc atttgaatca agtgggacgc tatagacttt
     4201 gtccttgatg gcgtattgtt cagcaacacc gtctttgata ttgctcatga aatctttacc
     4261 tgtcaaatct tcaaaaacat tattggctgc ccattctttg aaatcagcat tttgtggaaa
     4321 aacattgaca atatctggtg tatcttttga agaaatacgt gtttttaaga cgttacctgg
     4381 atcgggaacg gaaacaactt taactttgat atccttgttt tctttctcaa agtctttagc
     4441 aatttctttc gtaatcgcta acgtttcttt cttctgagtg aagaattcaa tttcagtttt
     4501 tttgctggct gagctaccac acgatgccag taaggcaact gacgctaaac tcaagaggga
     4561 cagtgtcaat tttgttcttt ttttcataaa aaggctcctt ctattgaaaa cacatttgta
     4621 accgttttct atatctttat tatatcacag aatactagga atgaatatag acaaaagttc
     4681 taaatatatg gtaaaatgta tgtagtaaac gtttacgaaa gaagatttag taaattatgg
     4741 aatacaaaga atttcatcaa aattttttag atatcaacct tgattttgta ggcaatgaag
     4801 ccactatccc caatttcagt ttcggtccag cgatacgcga aaactatgtt atccattata
     4861 tcacatctgg ctctggtcgc tatatgatct atggtttcga gcatcaatta aaagctgggg
     4921 attgttttat catacccgcg gatgtggaaa cattttacca atctgacgca ctgacgcctt
     4981 gggcctatta ttggcttgga ttaagtggtc atgttgtcaa tgatttattt gcgcgaactg
     5041 ccttagatga taagggttgg atattagaaa atgtcagtaa aacagaattt atagaacatt
     5101 tctctaagat tcaaaattta atttctgacg atgataagac cgttgattta gacattcaag
     5161 tagaactttt tgcacttatg aaaagtttaa ttaccctttt cccaaaatcc attaccgagc
     5221 ataagaacca atctgattat tatgctgaaa aagcctatac tttcatcaat caaaactata
     5281 gtcaaagcat taaaataaaa gatgtcttag cccatgtgat gatttcgcgc gcttatctgt
     5341 ttactatttt taaacataaa tatggtctgt caccccaaaa atatttaatt gacctccgca
     5401 tggccaaagc cgcaatgctg ctcattcact cagacaacct tgtctctcaa atttctgaag
     5461 ctgttggctt ctcggactcg cttagttttt caagtgcctt caaaaaaagg tatggcgtca
     5521 gtccaacaaa atttaaaacg caaaaacatg ataatttaat gttagaaaca ctaaataata
     5581 tgaatctaaa tcgactcaaa aaataagcca ggagcagctc taaactgctt ctggcttatt
     5641 ttgatacacg taatacgtaa tatatcaggc taatttctta atgacggaaa tgacgtgccc
     5701 ctgtgaagac catagcaatc ccatgcttgt ctgctttatc aatggcgtct tggtcacgga
     5761 ctgatccacc gggttggata atcgctttga tcccagcttt ggcaatttct tcaacattat
     5821 ctgcaaatga gaagaaggca tctgatgcta agactgcgcc ttcaagtcgg tctgaggcct
     5881 gctcaatcgc aatcttaaca gaagcgacac ggttggtttg acctgggcca acacctaatg
     5941 tttggtggtg attggtgata ataatgccat tagatttgac atatttgaca gaacgccatg
     6001 caaactcaag cgcttcaact tccactgctg ttgggtggcg tttggttaca atttcccaag
     6061 actcttgcaa ctcaacagtt gtatcttgat cttggacaag aagaccacct acaacacccg
     6121 tcacttcttt tggcgtttca gaggcttttt gttgcgcaaa gttcaatact aaaatccgaa
     6181 gatttttctt cttattggtc aaaatggcca aagcatcatc agaataactt ggtgcaatga
     6241 tgatttcaag gaaaatcgga ttcatttttt cagctgttgc ggcatcgact tcacgattta
     6301 agacgacaat cccaccgaag attgaaacgg ggtcagcatc gtaagcgtag tcccaagctg
     6361 tttcaatcgt ctcagctgta ccaatcccac aagggttcat atgtttcaaa gcgacaactg
     6421 tcggcaaggt gaaatcacga atgatgcgaa tcgctgcgtc agcatcacgg atattgttga
     6481 atgacagttc tttaccgtta agctgtttgg cttgtgaaat agaatagtct atcggaaggg
     6541 ctgactcata gaagtccgca ttttgttggg gattttcacc ataacgcata ggttgtttga
     6601 gatcataagt cagggtgagt ttctcaggct taacttcacc aacttgcgct gtgaaatatt
     6661 cagcaattaa agcatcataa gcggctgtgt gacggaaaac tttggctgct aatttttgac
     6721 gcgtttcata agtcgtgtca ccattttcag caaactcagt taagacaagc tcatagtctg
     6781 tcgggtcaac aacaacggtt acactggcat ggtttttcgc agccgaacgt aacatagagg
     6841 gaccgccgat gtcgatgttt tcgacagcat ccgcataggt cacatctggt ttaagaatcg
     6901 tctctttgaa aggataaagg ttgacgacaa cgaggtcaat cggtaagatc tcatgatcct
     6961 tcatcgctga aacgtgtgtg tcgaggtcgc gacgcgccag aagaccacca tgaattttag
     7021 ggtgcaaggt tttcacgcgc ccatccatca tttccggaaa acctgtgacg tcatcaatgg
     7081 caatggttgc aacacctgca gcatccagtg ctgttttagt cccgccagta gaaataattt
     7141 cccaacccag ttcacgcaat ttttcggcaa atgccacaat ccctgtttta tcacttacgc
     7201 taatcagtgc tcgttttgtc aatgtctcac tccttaaata ttagttgata agttcatttt
     7261 aacgatttat ccccaatttt gatagcgaaa aaacgtatat ctctgtaaaa tatacgttta
     7321 tcgttcagaa attactgaaa acgtgattaa actgccccca attcagcaat aaagcgcgca
     7381 aaggcagcat tgccatctag tgtatcctta aagaccccag cgtgctctaa gactgtcaag
     7441 aaaacgtggc ctaaggctgc ctgcatataa gtatctatcg cttcattttg gtagccttgc
     7501 ttcaaggctt cggcccatgg ctgatggtag gcagcaacgg tgtcggcttc gccaaggata
     7561 taacgtttga cctcctcaag ctctgttttc agtcgtcctg gtaaaatcgc aagtcccatg
     7621 acttcaatca agccgatatt ttcacgctta atatgttgaa catcgctgtg tgggtgaaaa
     7681 atgccatccg gatgttgctc actcgtgtta ttatcacgta aaacgaggtc aatctcgaaa
     7741 tcagcaccgc ggcgacgtgc gataggtgtg atggtatgat gtggggtgcc atctgctgtc
     7801 cttgcgacaa tttcaaccgt ttcgtcagtg tactgctgcc atttttctag gataaacgta
     7861 ctggctgcca ccaaatcttg gcggttatca gctgtcaagc gaatcacgct catgggccag
     7921 ttgacgatgc cagcacctac agtcggaaaa gtcggtatga tgaactgacc tttaagttca
     7981 gccttagcca tgggaaaatc gtgacgcccc gcctgataat gatcgtggct aagaatcgag
     8041 ccgccgacga tcggcaaatc agcatttgac cctgcaaagt aagtcggtaa actgtcaaca
     8101 atcgccagca agtgcgcaaa ggtcacttga gaaatcttca tcgggctgtg cttttcagca
     8161 agaataatcg aatgttcgtt aaaataagca tagggcgaat attggaaacc ccaagtctcg
     8221 ccagccaagt caagctgcac aatccgatga ttggtccgtg ccggatgatt gagcctgccc
     8281 gcatagcctt cattttcgat gcagagcaaa cacttaggat agctcgactg tacggcattt
     8341 ttttccgccg caatctgctt gggatccttc tcaggcttgg ataaattgat cgtaatttcg
     8401 agttcgccat aaggacttgc gctgtaaaaa tgttcatttt tggcaatggc gcgtgtcttg
     8461 atataatcgt ttttcttgga taagtcgaaa aaataatcag tcgctgcttt tggcgattgg
     8521 gcatattttt ctttaaaacg tcgattgaca tgagccggtg ttggggtcaa aaagttcatc
     8581 agcttcgctt gaaaaatgtc ttcatttgca gctatttcat caaattgacc atttgcttta
     8641 gcgacagcac aaaggaagtc agataaggtg agcgagtcgt cattgccaga tggttgaaga
     8701 ttttctgcta aatcatcttc accgattaaa gccaatatca aattttgtaa gtaaatgcga
     8761 tcggccactt cgtaagctcc tagcttgatg gcaagatctg cgaagtcaat cactgcttgt
     8821 gagatgttca taattttcta gctccatctg caatcgtcgc aacgtaaaaa ctggcagcat
     8881 atccgatttt ttcaaggtaa gttgctgaca gtttcgtaat aaaagtctcg atttctgatg
     8941 ttgcaacaag ggcaatgcca cagccaccaa atccagcacc tgtcatccgt gcaccaagaa
     9001 cccctgacaa accttgtgct gtttcagcca aggtgtccag ttcgatcccc gtcacttcat
     9061 agtcattttt caaggactca tgtgaagcgt tgagtaattg accaaatgct tgtaactcac
     9121 cagcttctag gcaagtctta gctaattttg tcctttcgtt ttcatagacg gcgtgtttgg
     9181 cacgcttttc taatgtcacg tctccaatca aggctcgatt ttcttcaaat tctgttgggg
     9241 tcaattgacc aagcgctgtg atctcaagct tcgtttgcaa acgcttgagt gcttcctcac
     9301 attcggcccg acgttcattg tacttagaat ccgctaattc tcggcgctta ttagtattca
     9361 tgatgacaat tttatagtcg ccaaattcag cagggacgag ttcatattta agtgtattgg
     9421 tgtctaataa aatcgctttg ccaatttcgc caaagccaat ggcaaattga tccatgatac
     9481 ctgaattaac gccaatgaat tcattttcta ctttttgacc gagctggact aaggttaatt
     9541 tatcaagacc cagatgatat aaatcattga caactgtccc aaccaataac tcaagagaag
     9601 ctgatgatga caagcctgaa ccattgggaa tcgtgccttc aatgacgagc tcaaaaccag
     9661 tttgaatggc atgacctgct tcttttaatt taagcagata acctttgaca aaatttcccc
     9721 agttggcttc tggtgtattg acaagctcat tttcaagtga aaatgcaatc acaccaactg
     9781 tttcaaaatt tagggaatag acttttacga gactatctgt gcgttttctt gcgacaccaa
     9841 aggtgccaat actgatggct gctggaaaga catagccacc attataatcc gtatgctctc
     9901 cgattaggtt aatctggcct ggtgagaaat aattggcttt tgcaccatca ccaaagaggg
     9961 ctataaattt tctgtgactt cttgtttcat tgcttgtgtt gacatacttg ttcctcactt
    10021 gatttcattt gatttgactt catttataac aaatttgcga ctttggtaat ccccgtctag
    10081 gaagggataa atataaagac caacagacat taattcatcc ccaccgtaga tagcatcacc
    10141 gttgacttgg taggttttct caggcgcaag tcctgtgaat ttaagcgtcc gaaggggacc
    10201 tgctgattct gcatattttc taaaataaaa accgatggct tgtgactgat caccattgac
    10261 aaaaagccat gctgtttgat ctgattcaaa tggtgataag agacgatgga attttccata
    10321 ctgcaagagt ttacggtgtg ttttgtagaa agtaacctgt tcaagaacga cttctttttc
    10381 tgcttgcgtc atgtcttgaa gattcaattc ataaccaaag acaccgctca tggcgacatc
    10441 acctcgaatc tctaaacctg tttcccgatg tgtttggtga ttgggcactg ctgatacatg
    10501 ggcacccatc gaagaaatgg gatagactag gctcgtgcca tactgaatct ctaggcgttc
    10561 aatggcatct gtattatcgc tcgtccaact ttgcggcatg taatgtagga agccagcatc
    10621 gaatctgccg ccgccacctg agcagccctc ccaaaggaca tgatcatagt ccgtcgtcag
    10681 ctcttctagc atctcataga ggcctaaaac gtatcgatgg aagacttcac cttggtgcgc
    10741 tgcacttgct gtcactgaat aaacttccgt caagttacgg ttcatgtccc atttgatgta
    10801 gtcaatgtca atcgtatcaa gaatagcccg catttgcctt gtaattgtct cgcgaacttc
    10861 ttttcgtgaa aaatcaagca cgtattggtc acgagaggta ctcatagaac ggtctggtgt
    10921 ttgaagggcg taatcaggat gttgacgaaa taggtcactg tccgcattaa ccatttcagg
    10981 ttcaaaccaa aggccaaagg acatcttctt ttgatgcaca tagtcagcaa tggctttaag
    11041 tccacctttc agcttctctg tattttcaaa ccaatcacct aaaccagata tatcatcatc
    11101 acgtttacca aaccagccat catctaaaac gaaaagttca atcccaagat cagctgcact
    11161 atcaacaatt ttcttgatct tatccgtgtc aaagtcaaaa taagtcgctt cccaattatt
    11221 gatgaggatc gggcgctccg cctgacggta ttgtccacgc gccaatcggt tttgaagtaa
    11281 gtcatgaaag gtttgactca tgccattaag tccttgatca gaataaacta acagggcttc
    11341 aggtgattgg aaagacgcat cgatcccaag ttcccattca aaaccaaagg gattaatccc
    11401 agccataaca cgcgtctgtg aaaattgatc tcgttcaact aacatttcat gattgccact
    11461 atagactaaa gttaaaccaa tcgctgtccc cgtcaattcg tctgttttag gcgcaacaat
    11521 cgcaataaat ggattagctt gatggcttga cgacccacgt ttactgtcaa tgattcgtgt
    11581 cccacgttga atcggctgac ggtcaatttc acgttctttg acatggcgtc caggcaaact
    11641 aattaattcc aagtcttgct caggtaaatc aacggatata ctcgctaatt tttcaatgac
    11701 aacaggtgct acgccatgat tttcaagcaa ggtattacga gctatcacat cgcgttgcgc
    11761 ataaatcgtg tacgaaagtt ttaatttgag attgagtagt ttgtcaacca gggtgatgac
    11821 aagtgtttca gcttcttcat cagcttctac ataggtcgct ggtaaaccat caagtatggg
    11881 tttgccagaa ataatctcat aactatcgta aacaaattca gtgataaatg agccattagc
    11941 ttgcttaatt tggacagcag gattgcggaa atcgccatga ccataacttg gaaattcttg
    12001 tgagatgaca tctagtgaaa aatcgcgtgt cttaagcggc atcccatccg gatttggtga
    12061 aaaactacga ttcatggctg gatattttcg gccaccatga taagttttaa tggctttacc
    12121 aaaataaaga tgactcaaat aactttcttt ttcaataccg ataagataag aaatggcggt
    12181 gtttgagagg tgaaaaattt ttttgctttc atcaaatgtg attagtgtca tgagagactc
    12241 gctttcttta tttactaaac gtttactgaa gtaattatat ctctttacga aagcgttgtc
    12301 aatgatttaa acaaaaaaga atttgataga ctgatcagcc tatcaaaccc tttaataact
    12361 attaagacag tttatttttc cggacactct ctcgtacaat aatttctgag gcgacttcaa
    12421 cacgtaacgc agtctttcga ccgtctgaaa ttcgttcttt agctagtcta acggccacac
    12481 gcccaatctc cagactatcg atatggacgg tactaagcgg cggtgataga tagcctacca
    12541 tctcaatatc atcaaaactg aagactgaaa tcgcttctgg tatcgtaata tttttcagct
    12601 gcagggcccg ataaatcccg atactcatgg gatcactggc ggaaataatg gctgttggca
    12661 actgatcgcc agaagcttca attaaggcat ttgtggcatt taaagcaaag cccatactcc
    12721 aatcacccgt tttaagttgg atcttatctg acaaaccatg aatggtcatc caattttcat
    12781 aggcagtggt cctaacatct gctaataagt gttgcttttg ccccttcaaa tcaaccgttc
    12841 taatttcacc accaatgaag gcgatttgcc gatgaccttg ttcgtaaaga taatctaaga
    12901 cttgttctgt ttgcttggca aaatcggtat gaacgaggtc atatcgagag ctagcaaaat
    12961 gattgtcaac gacaatcaga ttctgattga ccttgtaaat atccgctagc gtctcatctg
    13021 taaaagcacc gatcgtaagg accgcaccga attgtgcgag ctgctcaagg ctcacttctc
    13081 ccaacttcaa cgtttcgagt ctaaaattcc agttacccgc ttcaagtgcg atgccttcct
    13141 gaatcaagcg aaaatacgga tcatcatttt cgcgtttgga tgaatgcgtc ttgatcacag
    13201 caatcgataa tttattagat tgcttagggg atttaggccc taaattttct actttgtgat
    13261 aattaagctg gttggctact ttaataattc tggtgcgggt tgctggtgaa acactcatgg
    13321 tttcatccgc atttaagact cgtgataccg tagcaggcga aacacctgct aattttgcaa
    13381 tttctctaat actggccatg gcttgcaatt cctttacttg ctgatgtgtc ttctgcttga
    13441 tctatcatct gctaccaata ttttacctta ttttagacgt ttagtaaaca aaaaagcaag
    13501 ctttaggctt acttctcttt atgttagggc atataggcca ttaacgctgt caaatccata
    13561 atgtcagctc gtaagttatc gccgatcgtt gtatctttaa tcaagcggcg tttaggcttt
    13621 tcttccaaga agttggttaa ggattcgtta cctgttccct tggccaaaat ctcctcaatt
    13681 gacgtaaaag gggcgtgggt aacgatttta aagccgtagg aatcgtttaa gagggaatag
    13741 cctgcgatac cggttgtttt ttgataggct ttactcatac cgccatctat caccatgagt
    13801 ttcccattgg ctttgatcgg actttcacca ctggcaacct tgacaggtgt atgcccgtta
    13861 atcacaacgg actgtgggtc attcaaacca aattctgcca agatcatgtc catgatttct
    13921 ggtttttctc tgtaggcaaa atagggattt tcgccttctt tatgtgtcgt tttatctgag
    13981 ataaaatagc gctcaaatgt cgtcatggac gagcgactaa ataaaggcga gcatttaccg
    14041 gcccaagcat accacatcaa atcagtcgag taatcatcgt aaatctcagt atggcgaatc
    14101 ccacgtctga tgtgctcttc aaacttatca agcaagggac gaccagaata gagagtgccg
    14161 ccaatgctca tcgataaaaa agtgccatcc tttttcaaag gaatacaacc atggtagagc
    14221 aaatgccggt tgtaaacaag ataagccgaa cctttttcga ctagaaactg catgtggcgc
    14281 tggaattttt cggatttctg taaagaggac aggatcgccg ccatgacgta ggcttcactt
    14341 tcggtcaact cgttaggatg atactgggag acggtctgga aacacgtgtt ttccagggga
    14401 taatctgtgt cattgagggt gatggtcttg tgcttatagt ctattttttc taatagatta
    14461 cgatctccca tttcgaactc agggcgacgc gcaatgattt ggccttctag cttgaactga
    14521 atcaccgtca aggcctgatg gaccttggtc aaacgcgctt gattttcgac ccctagcaaa
    14581 tcatcatccg gacgtgctgt cggcttaaaa gccgagttca gctcataatg ttcttcagca
    14641 aacaagaaca agggccgcag attcagggaa taggccgttt ctaactcata gagatagtta
    14701 tacttcaccg caatcctaag cagcgtcgca agactggcca tgttcccagc ataagccccc
    14761 atccagagta catcatggtt gccccactgg aaatccacat tatggaactg aatcaaacga
    14821 tccatcacct tatccgcgcc cgcaccacgg tcaaagacgt ctcccaccac atgcagatga
    14881 tcgatcgtca actgctgaat cgtcatacac aaacttcgaa taaacttatc cgcctgattc
    14941 agctcgatca agcgcttaat gatctgctga taataatccg ctttctcaga ctgattcgtc
    15001 tctaaactca gcattaattc ttccgtaaaa tagcggaagt cttcatgaac cgccttacga
    15061 accttagaac gggagtattt agtccccaga aaacgcagca gcttaatcag acgatgaatc
    15121 gtttgactcg accaatcgtt ggcagcctcc aaatcctttg gcttacgttg caacatatca
    15181 gtcggataag caatcagcat ggccagacga tccatttctt cagggtcttc atccccaaaa
    15241 atcgcatcaa ttttctcttt aatattccca gcacctgtcc gaatgatgtg gttaaaagcc
    15301 ttgtattccg catgaatatc actgatataa agctccgtcc ccttaggcaa attaaggata
    15361 gcttccaagt tgatcagctc actcgcgacc ttgtccttgg tctcatattt ttcagctaat
    15421 aatgacagat atttgttttc ttgcat
//
