LOCUS       AB669415                4645 bp    DNA     linear   BCT 28-MAR-2012
DEFINITION  Microbulbifer sp. JAM-3301 DNA, inulin operon, sacA, pfk, inu genes
            for beta-fructofranosidase, frucrokinase-like protein,
            endo-inulinase, complete cds.
ACCESSION   AB669415
VERSION     AB669415.1
KEYWORDS    .
SOURCE      Microbulbifer sp. JAM-3301
  ORGANISM  Microbulbifer sp. JAM-3301
            Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales;
            Microbulbiferaceae; Microbulbifer.
REFERENCE   1
  AUTHORS   Kobayashi,T., Uchimura,K., Deguchi,S. and Horikoshi,K.
  TITLE     Cloning and Sequencing of Inulinase and beta-Fructofuranosidase
            Genes of a Deep-Sea Microbulbifer Species and Properties of
            Recombinant Enzymes
  JOURNAL   Appl. Environ. Microbiol. 78 (7), 2493-2495 (2012)
   PUBMED   22286980
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Uchimura,K. and Kobayashi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2011) Contact:Kohsuke Uchimura Japan Agency for
            Marine-Earth Science and Technology, Institute of Biogeosciences;
            2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Microbulbifer sp. JAM-3301"
                     /mol_type="genomic DNA"
                     /strain="JAM-3301"
                     /db_xref="taxon:1081041"
                     /country="Japan:Kanagawa, Sagami Bay,water depth of 900 m"
                     /lat_lon="35.08 N 139.21 E"
     operon          <1..>4645
                     /operon="inulin"
     gene            1..1458
                     /gene="sacA"
                     /operon="inulin"
     CDS             1..1458
                     /gene="sacA"
                     /operon="inulin"
                     /codon_start=1
                     /transl_table=11
                     /product="GH32|CBM38"
                     /protein_id="BAL70276.1"
                     /translation="MMDLEVETGVVELPQPESGFRPHWRPKRHFTPAKNWMNDPNGMVY
                     LEGVYHLFFQYNPGDSVWGDIHWGHATSTDLLHWQEQPVALASEPDGLGYIFSGGAVVD
                     WENSSGFQSGDQPPLVATFTHHSKEEVQAQSLAVSTDGGLSWDKYPHNPVIPNPGIKDF
                     RDPKVIWHRDSGHWVMVLAVGDQVHFYRSRDLKDWVFTSGFGRTSGAHGGVWECPDLFP
                     LRDADGEERWCLLVSINPGGPNGGSATQYFIGGFDGEAFVPEHEDIRWLDYGTDNYAGV
                     TWDGLQRVDDRRLFIGWMSNWQYANKVPTSLWRGAMTIPRELSLYRDKGALRLANAPAR
                     EVDEARRRVVSMPLQPGNSIALPGNSALDLVLKIGGAPELELEFSAGENGGRLLLALSA
                     ERGALLLDRRESGWVEEGFGREISAPLAHALHGESELRILIDGSSVEVFIDDGRTAVTA
                     LFFSSAPLSHMVLCRADAAVPFVLYRL"
     gene            1469..2422
                     /gene="pfk"
                     /operon="inulin"
     CDS             1469..2422
                     /gene="pfk"
                     /operon="inulin"
                     /EC_number="2.7.1.11"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|PfkB"
                     /protein_id="BAL70277.1"
                     /translation="MTELRVSVFGEALIDLIETPGGYFEPHVGGSPFNVCRGFARMGLA
                     CDYLAPISSDRMGDRIAAAALAESIDIGRVARSPRPTSLALVSIGNDGQPNYRLYREGV
                     ADLDIDTKALVDAVHPQCRLFHSGSLALVAEMEATLAEVFARLHARGIPVSIDANLRSG
                     VARDTGAYVAAVKRLIGCADILKVSDEDLQLLGVREPLGFCEALLRDGQARLVAFTEGA
                     LGATLISGQGRVQQPAELPETLGDTVGAGDTFFAALLSYLLHNDLLQGGDAALDSAALG
                     RGLAFAQRAASINVSRRGCSPPTLGEVQCVFATGEV"
     gene            2426..4645
                     /gene="inu"
                     /operon="inulin"
     CDS             2426..4645
                     /gene="inu"
                     /operon="inulin"
                     /EC_number="3.2.1.7"
                     /codon_start=1
                     /transl_table=11
                     /product="GH32"
                     /protein_id="BAL70278.1"
                     /translation="MGMPVLHRLFGFLFLAFPAPLLVAAEEPPAYRQDFERLDTEALAP
                     LRIHTQFSAPDSVPGVAGKAWRSDGFSSWAELPYETGEKFSLTVWLALESYPSDLEVPV
                     SELSPSAIVHQWDGRGGFNIHVDTFGRWGARLQTESGETDLVVETPFPLYRWVQLALSV
                     NEREAALYLDGEMVAREVLPAPLRPADAPIRLARAVDEAEILNFTVNRLNGVFDELRLY
                     RRGLPAAEIQAIRRRYPDTVPVEPSLAVPESRFAGDHLRPRLHAMPPANWTNEPHGLVK
                     VGDLWHLFYQRTPNGPYKTQMHWGHMVSRDLLRWENLPDALWPELQDDEFGFDMKGIWS
                     GDVIYEGGRAFAYYTSVNHGDRLGAANPGVSMAVSEDPELRHWRKLGPIINTRYVRDFR
                     DPYLFKDGDTWHMLIGAAQKDGGGLDYYILEKEENKWRHRRRFSSIPFHKMDIGSEIWE
                     MPVFEPISVDAHVLVVNPIGGRISKYGEPATRAVYWVGRWRNGLFHPYESEPKNLDLLP
                     GHLAPAVERGEDGSLRAIGIVDERRSPQAQEDAGWAHTFSFPRRWRLLEDGRTLGQSPA
                     PELVRLRGEPLDTPPVPALLEKPLWLAEPDGAYELVLESSGAGGLAIGVDLLVSSAGGE
                     YTRLMFDGESDTVILDKRNSWHGGGGEGPDLLQAAYDSAAFGEIRELRVFVDGSVVEVF
                     VNDSAAFSFRSYPEDPGARGLRIFPLRKKAVLAGATLWPLRIPAE"
ORIGIN
        1 atgatggatt tggaagttga aaccggcgtg gtggaactgc cgcaaccgga aagcgggttt
       61 cggccccact ggcggccgaa acggcatttc actcccgcga aaaactggat gaacgacccc
      121 aatggcatgg tttacctgga gggtgtctac cacctgttct tccaatacaa cccgggtgac
      181 tcggtgtggg gggatattca ctggggacat gccacctcca ccgacctgct gcactggcag
      241 gagcagccgg tggcgctggc gtcggagccc gacgggctcg gttatatctt ttccggcggc
      301 gcggtggtgg attgggaaaa cagctccggt ttccagagtg gcgatcagcc accgctggtc
      361 gccaccttca cccaccactc caaagaggaa gtgcaggccc agagcctggc ggtcagcacc
      421 gacggcggcc tgagttggga caagtatccc cacaacccgg tgatccccaa tccggggatc
      481 aaggattttc gcgatcccaa agtcatctgg catcgggaca gcggtcactg ggtgatggtg
      541 ctggcggtgg gtgatcaggt gcatttctac cgttcgcggg acctgaaaga ttgggtgttt
      601 accagcgggt tcggccgcac ctccggagcc cacggcggtg tctgggagtg cccggatctg
      661 ttccctctgc gggatgccga cggggaagag cgctggtgtc tgctggtgag catcaatccc
      721 ggcggtccca acggcggctc cgccacccag tactttatcg gtggtttcga cggcgaggca
      781 ttcgtgccgg agcatgagga tatccgctgg ctggattacg gtaccgacaa ttacgccggc
      841 gttacctggg acgggctgca gcgcgtcgac gaccgacggc tttttatcgg ctggatgtcg
      901 aactggcaat acgccaataa agtgccgacc tccctgtggc gcggagctat gaccattccc
      961 cgcgagttgt ccctctaccg ggacaagggt gccctgcgtc tcgccaatgc gccggcgcgg
     1021 gaggtggacg aggcgcggcg gcgggtggtg agcatgccat tgcagcccgg caattcgata
     1081 gcactgccgg gaaacagtgc cttggacctg gtcctgaaga tcggaggggc gccggagctg
     1141 gaactggaat tctccgccgg agaaaacggc gggcgattgc tgctggcgct ttccgccgaa
     1201 cgcggcgcac tgctgctcga ccggcgtgaa tccggctggg tggaggaggg cttcgggcgc
     1261 gagatttccg cccccctggc gcacgcgctg catggggaga gcgaactgcg catcctgatc
     1321 gacggcagct ctgtggaagt ctttatcgac gacggcagga cggcggttac ggcgctgttc
     1381 ttttccagcg cgccgctgtc ccatatggtg ttgtgccggg cggacgcggc cgtccccttc
     1441 gtgttgtatc gcctgtaggg gaggttccat gactgagctg cgcgtttctg tgtttggcga
     1501 ggccctgatc gacctgatcg aaacacccgg cggttacttc gagccccatg tcggcggctc
     1561 cccgttcaac gtatgccggg gctttgcccg catggggctg gcctgcgact acctggcccc
     1621 tatctccagc gaccggatgg gggaccggat tgcggccgcg gcgctggcgg agtcgatcga
     1681 tatcggcagg gtggcccgct ctccccgccc gacgtcgctg gcgctggttt ccatcggcaa
     1741 tgacggccaa ccgaactacc gcctgtatcg ggaaggcgtc gcggatctgg atatcgatac
     1801 gaaagctttg gtagacgctg tgcatccgca gtgccgcctg ttccacagcg gctccctggc
     1861 cctggtggcg gagatggagg ccacgctcgc ggaggtattt gcccgactgc acgcgcgggg
     1921 aatccccgtc agcatcgacg ccaacctgcg cagcggagtc gcccgcgata cgggcgccta
     1981 cgtggctgca gtgaagcggt tgatcggctg cgccgatatt ctcaaggtca gtgacgaaga
     2041 cctgcaactg ctcggcgtgc gcgaaccgct ggggttttgc gaagccctgc tgcgcgacgg
     2101 gcaggcccga ctggtggcgt ttaccgaagg cgcgctcggt gcaacactga tttccgggca
     2161 aggacgggtg cagcagccgg cggagttgcc ggagaccctt ggtgataccg tgggtgccgg
     2221 ggatactttt tttgctgcgc tgctctccta tctgctgcac aacgaccttc tgcagggcgg
     2281 tgacgccgcg ctggattccg ccgctcttgg aaggggtctg gcttttgccc agcgggcggc
     2341 gtcgatcaac gtctcccgcc gtggttgcag tccgcccaca ctgggggagg tccagtgcgt
     2401 ttttgcgact ggggaggttt aggagatggg tatgccggtc ctgcacagac ttttcggatt
     2461 tcttttcctg gcatttcccg ccccgctgct ggtggcagcg gaagagccgc cggcgtaccg
     2521 ccaggatttc gagcgactgg atacggaggc gctggcacca ctgcgcatcc atacgcagtt
     2581 ttccgcaccg gattctgtac cgggggtggc tggcaaggcc tggcgcagtg acggattctc
     2641 cagctgggcg gagctgccgt acgaaaccgg cgaaaaattt tccctcactg tctggctggc
     2701 gctggaaagc tatccctcgg atctggaggt gccagtgtcc gagttgtccc cttcggcgat
     2761 tgtccatcag tgggacggca gaggcggttt caatatccat gttgatacct ttggacgctg
     2821 gggcgcgcgc ctgcagaccg aaagcggaga aacagacctt gtggtggaaa caccgttccc
     2881 tctttaccgc tgggtacaac tggcactctc cgtgaatgag agggaagcgg cgctgtatct
     2941 cgacggtgag atggtcgccc gcgaggtact gcccgctccc ctgcgcccgg ccgatgcgcc
     3001 gattcgactg gcgcgggcgg tcgatgaggc ggaaatactc aatttcaccg taaaccggtt
     3061 aaacggcgtc tttgacgagt tgcggctata ccggcgcgga ctccccgcgg cggaaataca
     3121 ggcgatccgc agacgttacc cggacacagt tcctgtagag ccttcactgg cggtgccgga
     3181 aagccgtttc gcgggtgatc atctgcggcc gcggttgcac gccatgcctc cggccaactg
     3241 gaccaacgag ccacacggct tggtgaaggt aggggatctg tggcacctct tctaccagcg
     3301 cacgcccaat ggtccctata aaacccagat gcactggggc catatggtga gtcgtgacct
     3361 gttgcgctgg gaaaacctcc cggacgcgct ttggccggaa ttacaagacg atgagttcgg
     3421 tttcgatatg aaaggcatat ggtccggcga cgtgatttat gagggcggcc gggccttcgc
     3481 ctattacacc agcgtaaatc acggcgaccg cctgggggcc gctaaccccg gcgtttccat
     3541 ggcggtgagt gaagatccgg aactgcgaca ctggcgcaag ctggggccga tcatcaacac
     3601 cagatatgta cgggatttcc gcgaccctta cctgttcaag gacggcgaca cctggcacat
     3661 gctgatcggt gcggcgcaga aggacggcgg ggggctggat tactacatcc tggaaaaaga
     3721 agaaaacaaa tggaggcacc ggcgcaggtt ctccagcatt cccttccaca aaatggatat
     3781 cggctccgag atctgggaga tgccggtatt cgagccgatt tcagtcgatg cccacgttct
     3841 ggtagtcaac cccatcggcg gccgcatctc caagtacggc gaaccggcca cgcgcgccgt
     3901 ctactgggtg gggcgctggc gcaatggatt attccacccc tacgagagtg aaccgaaaaa
     3961 cctggatctt ttacccggcc acctcgcgcc ggcggtggag cggggagagg acggttccct
     4021 gcgtgcgata ggaatcgtcg acgagcggcg ttccccccag gcccaggagg acgccggctg
     4081 ggcgcatacc ttcagcttcc cgcggcgctg gcggttgctc gaggacgggc gcacgctggg
     4141 gcaatcgcca gcgccggagt tggtgcggtt gcgaggtgag ccgttggata ctcctccggt
     4201 gccggcgctg ctggagaaac ccctgtggct ggcggagccg gacggggcct atgagctggt
     4261 gctggaaagc agtggtgcag gcggcctggc aatcggtgtg gacctgttgg tttcgtccgc
     4321 gggcggtgaa tacacacgcc tgatgttcga tggcgaaagc gatacggtaa tcctggacaa
     4381 gcggaactcg tggcacggcg ggggcggcga ggggccggat ctgttgcagg cggcatacga
     4441 cagcgcggcc ttcggcgaaa tacgggagtt gcgggtattt gtcgatggtt ccgtggtgga
     4501 ggtgtttgta aacgatagcg cggccttttc cttccgcagt taccccgaag accccggtgc
     4561 gcgcggcctg cgtatctttc ccctgcggaa aaaggccgtg ctggccggcg cgacactgtg
     4621 gcccttgcgg ataccggcgg agtga
//
