LOCUS       NZ_ABQA01000009        13682 bp    DNA     linear   CON 04-FEB-2020
DEFINITION  Enterococcus faecium E980 Contig00009, whole genome shotgun
            sequence.
ACCESSION   NZ_ABQA01000009
VERSION     NZ_ABQA01000009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02471501
            Assembly: GCF_000172615.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecium E980
  ORGANISM  Enterococcus faecium E980
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   van Schaik,W., Top,J., Riley,D.R., Boekhorst,J., Vrijenhoek,J.E.,
            Schapendonk,C.M., Hendrickx,A.P., Nijman,I.J., Bonten,M.J.,
            Tettelin,H. and Willems,R.J.
  TITLE     Pyrosequencing-based comparative genome analysis of the nosocomial
            pathogen Enterococcus faecium and identification of a large
            transferable pathogenicity island
  JOURNAL   BMC Genomics 11, 239 (2010)
   PUBMED   20398277
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  AUTHORS   van Schaik,W., Top,J., Bonten,M.J.M. and Willems,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2009) Department of Medical Microbiology,
            University Medical Center Utrecht, Heidelberglaan 100, Utrecht 3584
            CX, The Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Genome Coverage                   :: 19x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/04/2020 07:40:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 2,759
            CDSs (total)                      :: 2,694
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 58 of 123
            Pseudo Genes (incomplete)         :: 42 of 123
            Pseudo Genes (internal stop)      :: 33 of 123
            Pseudo Genes (multiple problems)  :: 10 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            ABQA01000009.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Enterococcus faecium E980"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Contig00009"
                     /strain="E980"
                     /isolation_source="healthy human volunteer"
                     /host="Homo sapiens"
                     /db_xref="taxon:529885"
                     /country="Netherlands"
                     /collection_date="1998"
     gene            complement(1..1461)
                     /gene="gtfA"
                     /locus_tag="EFME980_RS04995"
                     /old_locus_tag="EfmE980_0216"
     CDS             complement(1..1461)
                     /gene="gtfA"
                     /locus_tag="EFME980_RS04995"
                     /old_locus_tag="EfmE980_0216"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH13|GH13_18"
                     /protein_id="WP_002290364.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFFP
                     STGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNHEQ
                     SPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEEQID
                     LDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLDEVRQ
                     EAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFASPMVTLYSLYSGKSERLANWLKMSP
                     MKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNLDIYQIN
                     STYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGRNINRHYY
                     DLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRKNKNGSDSA
                     TLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            complement(1458..2441)
                     /locus_tag="EFME980_RS05000"
                     /old_locus_tag="EfmE980_0217"
     CDS             complement(1458..2441)
                     /locus_tag="EFME980_RS05000"
                     /old_locus_tag="EfmE980_0217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH13_31|GH13"
                     /protein_id="WP_002290365.1"
                     /translation="MWLKEMRERTFKPHNSMTVAEADVPNERLDEYIGEDGYYSMVFDF
                     SYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQDKGWGALYLENHDQPRSINKYIP
                     EEWINDYSKKMLATLFMLLRGTPFIYQGQKLGMTNIEMASLEDFDDVATHSQYKRALEY
                     GLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFSTSDNVWLKVNPNYPELNAEAQLTNQ
                     QSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKDKNEHILLYERQLEDKIVLVILNVTAT
                     EQEYYVDEQYQQVLINNYEDVLLDQSRKLRLRPFESIVLANYGGQL"
     gene            complement(2522..2677)
                     /locus_tag="EFME980_RS05005"
                     /old_locus_tag="EfmE980_0218"
     CDS             complement(2522..2677)
                     /locus_tag="EFME980_RS05005"
                     /old_locus_tag="EfmE980_0218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="WP_002290366.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDAI
                     LNIKKN"
     gene            complement(2738..4921)
                     /locus_tag="EFME980_RS05010"
                     /old_locus_tag="EfmE980_0219"
     CDS             complement(2738..4921)
                     /locus_tag="EFME980_RS05010"
                     /old_locus_tag="EfmE980_0219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36"
                     /protein_id="WP_002290367.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSGG
                     YRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDFRY
                     DSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVIIRST
                     QLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLDSKRG
                     SSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGINSFNFG
                     WQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLINNWEATY
                     FDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLACGLENLAK
                     QVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDFSRQDVRDN
                     IYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLYEFLEKLTTN
                     YPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVYPISSMGAHVS
                     AVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAFYKKHRQLLQYG
                     TFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLAGLAEEATYQMED
                     VTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            complement(4961..5791)
                     /locus_tag="EFME980_RS05015"
                     /old_locus_tag="EfmE980_0220"
     CDS             complement(4961..5791)
                     /locus_tag="EFME980_RS05015"
                     /old_locus_tag="EfmE980_0220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00751|3.A.1.1.28"
                     /protein_id="WP_002290368.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNFF
                     RFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKAYG
                     LMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFIKLN
                     VPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILNRDSN
                     MWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK"
     gene            complement(5803..6675)
                     /locus_tag="EFME980_RS05020"
                     /old_locus_tag="EfmE980_0221"
     CDS             complement(5803..6675)
                     /locus_tag="EFME980_RS05020"
                     /old_locus_tag="EfmE980_0221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00750|3.A.1.1.28"
                     /protein_id="WP_002290370.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTGL
                     TYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGKTF
                     FRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVIFVL
                     LWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFIMALK
                     GGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGIVSLAQ
                     LKLSKKFEI"
     gene            complement(6685..7944)
                     /locus_tag="EFME980_RS05025"
                     /old_locus_tag="EfmE980_0222"
     CDS             complement(6685..7944)
                     /locus_tag="EFME980_RS05025"
                     /old_locus_tag="EfmE980_0222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00749|3.A.1.1.28"
                     /protein_id="WP_002290371.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKEM
                     TQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEWAK
                     AGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQAPET
                     WAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSEVNGI
                     NAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAMPMIHS
                     QNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPEAMQKYY
                     DVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTTNYLHNGN
                     QKAMINALNAFFNPMKADGR"
     gene            complement(8093..9403)
                     /locus_tag="EFME980_RS05030"
                     /old_locus_tag="EfmE980_0223"
     CDS             complement(8093..9403)
                     /locus_tag="EFME980_RS05030"
                     /old_locus_tag="EfmE980_0223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH4"
                     /protein_id="WP_002290372.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLKE
                     SNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKKYG
                     LRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLKATS
                     IKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDFYPTI
                     RQLAAEKPNPHPDSVRFELLKRFGYYITESSEYNAEYHPYFIKSKYPELIDELGIPLDE
                     YLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNVLNKGLI
                     TNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALTLDKSKIY
                     QAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            complement(9435..10259)
                     /locus_tag="EFME980_RS05035"
                     /old_locus_tag="EfmE980_0224"
     CDS             complement(9435..10259)
                     /locus_tag="EFME980_RS05035"
                     /old_locus_tag="EfmE980_0224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q93J92|3.A.1.1.36"
                     /protein_id="WP_002290373.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIPP
                     TIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPGKN
                     ILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFLGLP
                     DELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSIDKMP
                     LAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGGKL"
     gene            complement(10275..11171)
                     /locus_tag="EFME980_RS05040"
                     /pseudo=""
     CDS             complement(10275..11171)
                     /locus_tag="EFME980_RS05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /protein_id="WP_008378728.1"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(11254..12363)
                     /locus_tag="EFME980_RS05045"
                     /old_locus_tag="EfmE980_0226"
     CDS             complement(11254..12363)
                     /locus_tag="EFME980_RS05045"
                     /old_locus_tag="EfmE980_0226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9WZR7|3.A.1.1.40"
                     /protein_id="WP_002290375.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMHS
                     SESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWYNK
                     ELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGGEVF
                     ADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMIEGNW
                     FAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASANTKYPEEVKKLMAFLASEDA
                     NKIQAETGASIPALKGFEDEWAASSDTIDLSTFGKMLEYGVPRPYNKDGLRAEQAEVQI
                     INRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            complement(12657..13682)
                     /locus_tag="EFME980_RS05050"
                     /old_locus_tag="EfmE980_0228"
     CDS             complement(12657..13682)
                     /locus_tag="EFME980_RS05050"
                     /old_locus_tag="EfmE980_0228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0044558"
                     /protein_id="WP_002290378.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNFY
                     HYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLSET
                     PLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKATNQ
                     HLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQIYLE
                     GWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLRIPEDISIVSFD
                     NIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVRESEKNI
                     D"
ORIGIN
        1 ttaaaatttt aatgcaacac tagtgaaaat ttttatatct ttagactcaa tgataaattc
       61 tttcgtatcc aagttagccg ttaatgttgc actatcacta ccgtttttat ttttacgtgt
      121 aatcactatt tctgattctg atggcgttgc tacttcaatc gttccatcca aatcaaacgc
      181 cgcagattca tttctaaatt ttaaaagtgc aaataattgt tgaatcactg gccgttgcac
      241 ttcttcttca atttcggcta aatcatagta atgacgatta atattacgcc cttctttcgt
      301 tgcttccaac agttcaatat cattttttcc tgctaataac ccaacgtaat aaatttgtgg
      361 aatccctggc gcaaatactt gaatcgcacg tgctaacaga tagctacggt cattatcgcc
      421 taatgcacta taataggtgc tgttaatttg ataaatatct aaattattgt aattggcact
      481 tgaatagacc ttcttcacat tcgccccgac tttatacaat tcctcagagg tgtaatccaa
      541 ttcttcatct gttaataaat cacgagcgtc cacaacacca attccatcat gcgtatccaa
      601 tgttgtaaac tgcttcatcg gactcatttt caaccaattg gctaaacgtt ctgattttcc
      661 actataaagt gaatacagag tgaccattgg cgaggcgaag tcatagatat aataatcatg
      721 attggcaatt ttcatttgaa tggaataatg ctcatggatt tctggtaata attcgacgtc
      781 atatttcgct gcttcttgac gcacttcgtc taaaagttcc caaatatctg gttccacgaa
      841 gaaatcattg gtatctaatt ttttaatcgc ataagcaaac gcatctaatc gaattaaaga
      901 acagccatgg ttcgccatat ctttgatggt ttctcgaata aattctttca caacttcttt
      961 ggtcacatct aaatcaattt gttcttcccc aaaggtattc caaacctctt ctgtcgtacc
     1021 atccgcaaat tctaccgttt gaaacggcgc tttgtcttta cgcttgtaga ttaaatcaat
     1081 atcttcttgt gttggtcgat tttctgggaa aaattcatga atacggataa acatttcttt
     1141 gtatggtgac tgctcatggt tttttttgaa atcttggaaa aattttgatt cacgagaaat
     1201 gtgattaatc ataaaatcaa acatcaaata ataatcttca cctaatgctt ccacatcttc
     1261 ccatgttcca agagctggat caactgtcgt ataatcactc ggcgcaaagc ctcgatcacc
     1321 tgtcgatgga aaaaaaggca acagatgtac accaccaata acaccttgta aatgtttgtc
     1381 taagacttgt tttaattcgc ccatgttttt tcctaaacta tctgcatagg tgatgagcat
     1441 tgcttcgttt ttaattttca taattgtcct ccataattag ctaatacaat actttcaaac
     1501 ggtcgtaaac gcaattttcg actttgatct aataaaacat cttcataatt attgattaat
     1561 acttgttggt actgttcatc tacataatat tcttgttctg ttgctgtaac gtttaagata
     1621 actaatacga ttttatcttc taattggcgt tcataaagca aaatatgttc atttttatct
     1681 ttcactggta gaaattgtcc gtagattagc acgtctttgt atacactatt ttttcttaat
     1741 ttaattaatt gatgataata attgaatacc gactgttgat tggttaactg agcttctgca
     1801 ttgagttctg gataattagg atttactttt aaccatacat tatcagaagt tgagaatctg
     1861 gcattttttt gcgtattcca ttgcataggt gtgcgagaat tatcccgact tcgtttattg
     1921 acagctttca atgcttgctc tggtgccaat ccatattcta atgcccgttt atactgagaa
     1981 tgcgtagcta catcatcaaa atcttctaat gatgccattt ctatgttggt cattcctagt
     2041 ttttgtcctt gataaataaa aggggtacct cttagtaaca taaaaagcgt cgccaacatt
     2101 tttttactat aatcattgat ccattcttct ggaatatatt tattaattga acgtggttga
     2161 tcgtggtttt ctaaatataa ggcgccccaa cctttatctt gcgtaactaa ttgattcgta
     2221 aaaatattgg tacgcatatc cgtccatgtc cattggctat ctttaaacca ttcaccagtt
     2281 tcaggtacat caatatccgt ataactaaaa tcaaaaacca tcgaataata gccatcttca
     2341 ccaatatatt catctaaacg ttcattagga acatcggctt ctgctaccgt catactatta
     2401 tgtggtttaa atgtccgctc tctcatttct tttaaccaca cttcaatgcc cgactgatta
     2461 agaatccagg gcccaatgaa ggctaaccca tcttctccat cagattcaaa gatgccatac
     2521 tctagttttt tttgatgttt aatatcgcat cgattctaaa tccgcctagt cctttatcta
     2581 accaatagtt aatcattgca tagatttctt cacgtacttc ttcattttcc caattcaaat
     2641 caggctgctt tttagtaaat gcgtgtaaat aaaacatatt ttcttcgttt ggaatgagtc
     2701 tcttttggtg ttgcacttat attgtaaatt caagctacta tatctgtttc aaataaaagc
     2761 gttgtgtcgc ataatcacca tgtagttctg agtcaatata aaaaccgaga ttcatcaatt
     2821 catcgccagc aaatgttaca tcttccattt gataagttgc ttcttctgct aatcctgcta
     2881 atttcaatgt gactaatgga tgcgcagctt ctgctaatac tcggaaataa aagaccatgg
     2941 cttctgtttg gtcttttgag acaaacatcc atgcagtatc attgccttca aacggacttt
     3001 ccaagcgata aaaagttcca tattgcaaaa gttggcgatg ctttttataa aaggcaactt
     3061 gttctttaat ttctgctttt tcttcagaag ttaattgcgt tagatctagc tcataaccaa
     3121 aaacaccagc catcgcagca ttgccacgaa tagttaaact cgtttctctt cctgtttgat
     3181 gatttgggac tgctgaaaca tgcgcaccca tacttgaaat tggatacact aagctggttc
     3241 catattgaat tttcagacga gccacggcat cggtattgtc gctcgtccaa gtttgtggca
     3301 tgtaatacaa gaaacctgca tcaaaacgac cgcctccacc cgaacaactt tcaaataaaa
     3361 tagttggata attcgttgtt aacttttcta aaaattcata taaccctaac atataacgat
     3421 gagccacttc accttgcatc gttgagtcca acaagacaga atagacttct gtcatatttc
     3481 gattcatatc ccacttcaca taatcaatcg gtacacgatc aaaaatcgcc gtcatttgtt
     3541 gatagatatt atcacgaaca tcttgtcttg aaaaatcaag aacatattga tttcgactaa
     3601 ttgagcggtc acgacctgga atacccaatg cccaatctgg atgtgctcga aatagttcac
     3661 tatcttcaga aatcatttcc ggttcaaacc aaatcccaaa tttcatcccg ttatcatgca
     3721 cttgcttggc taaattttcc aaaccacaag caagtttgcc ttctgtttca acccaatcac
     3781 ctaatgaagt gaagtcattt tcacgaccgc caaaccaacc atcatctaaa acaaacattt
     3841 caatgcccaa gttcttcgca tcatcgacaa tattcatgat tttttcttcg ttaaaatcaa
     3901 aatacgttgc ttcccagttg ttgattaacg tgggacgttc ttttaaacga tagtttccac
     3961 gaattaaacg gtgattatac aattcatgat acgtctgtga taacgtattc agaccttcat
     4021 tactataaac catcacgact tctggcgttt gaaatgagtc gccagttttt agttgccaac
     4081 caaaattaaa tgaattaatt cccataatga cccgtgtttg gtcaaatgga tctttttgaa
     4141 tcaccgtttc gtgattaccg ctataaacta aattaaatcc atatgcctct ccttgaaatt
     4201 catctgtttt ggcatctact aaagcaacaa acggattttg ttgatggctg cttgatccgc
     4261 gtttactatc taatactttt atccctgtat gaattttttc tctagtcatt tgacgttctc
     4321 ttgcccaggt accatttaaa tgaattaact cgagtggacg atttggaaaa tcaattgatt
     4381 gactagctaa gcggttaatc tccactgttt catcactatt atttactaat tgcgtagaac
     4441 gaataattac actgtgattt tgataaattg tgtagaacaa ctgtaaagtc aatccactaa
     4501 tcgcgtcttt caattgaata atcagcgttt cagcttcatc atcatcttcc gtatataagg
     4561 ctggcaatcc cgctaagctg ggctttcctt tttgaatttc gtagctatca taacgaaaat
     4621 catttacacg actaccgttt cctaatttaa tattatgcgc cggttctcta aaatcaccat
     4681 gtccatatcc tggaaattct aatggtaatg tatctaaaga taataaacga tcttgcgcat
     4741 ttgcattgtt gggtgagaag gaacgatctg ttcgggggta gcgataccca ccagaatagt
     4801 gcgtaatctt ttttccaaaa taaagatgcg ttaaataatt attttcttcg attcctaata
     4861 gatagctaat ttttttattt tgtaaatgaa atgtttgtgt gtgattgtca aatataatca
     4921 taagtttatg aactccttta taatgagaat aatttattgg ttatttcact gctccattgc
     4981 tcattccagc tataatactt ctttggaaag caagataaac tagtgtaatc gtgacaattc
     5041 ctactgtata ggaagcaaaa cttggaccat agtcgttaaa atattgtccc gtataattat
     5101 attgaaacaa tggtagtgtc cacatattag aatcacgatt taaaattaat aatggtagca
     5161 tgaaatcatt ccaaaaccat agtgcattaa gaataatagt cgtcgcatgc attggtttta
     5221 acattgggaa aacaattttg aaataagttg taaagtgact agccccatca atttcagcag
     5281 cctcatccag actgataggc acatttagtt taataaaacc aacatataaa aatagtgtct
     5341 gtggtacggc ataagtgaga tagagtaaga ttaatcccca catatttgct aaaccaattt
     5401 tactcatcat aacggtaatc ggaatcataa ttacttggaa aggaacaaaa attcctaaaa
     5461 ttaaaaataa atacatcaat ccgtatgctt tcccacgagt catttttctt gctagtgaat
     5521 aagcagccat aggaataatt gctgtcacag caactactgc aatgattgtg atgattgccg
     5581 aattccaaaa gtaatgacca attccatccg ctaacaatct tcggaaattt tccaaagtcg
     5641 ctggattagg aaaacgaaag aaattattca taatatcggg tgttgtttta aatgaaccga
     5701 atagcgttga aactaaggga actaaaatca tgaagccccc taataataaa agcaagtatt
     5761 tagaaatatt tttttctttt gatgttttca ttgggaaacc tcctagattt caaatttttt
     5821 cgataatttt aattgtgcca gtgaaacaat cccaattaag ataaataaga ctaaagcaat
     5881 agcattcgca taaccaaact gattgttttt aaaagcataa ttataaacaa gtaaccccaa
     5941 agatgttgtg gcatcgcttg gtccaccacc agttaaagca aaaatttgat caaatgctgt
     6001 taagccacct tttaatgcca taataaatac cattgaaata cttggtaata agtaggtgat
     6061 ttcaatattc caaaaaattt gcttatcatt cgccccatcc attctagcag cttcaataat
     6121 ttcttgtgga atactttgaa aacccgctaa gaaaatgatg actggcatgg caattccttg
     6181 ccaaagtaaa acaaaaatta ctgcaaaaat cgctccccat gttgttccta aaagactggt
     6241 ttgtaaaaat tcaatgccta acatactacc aatttttggt aaaccatagt taaaaatttg
     6301 tttaaatact aaagaaacaa ccacgcccga taagaccgct ggaaaaaaga accatgctct
     6361 aaagaatgtt ttccctttaa tatttgcgtt caacgcccgc gcaatcaaaa taccaaaacc
     6421 aatttgtcca gcaatcatgc aaagcgtcac aattaaagtg aagccaatcg atttgataaa
     6481 cttcggatca gaaaaaagaa tcttataatt gttcaaaccc acaaaatcaa aattataagt
     6541 cagaccagtc caattagtta aactgtaaaa agcaccttga accattggaa agtagaaaaa
     6601 aatcatctgc aaaataattg gaattgctac aaaaatgatt gcccagtaac gatttaaaat
     6661 ttcttttatt ttcattttat tttcctacct tccgtcagct ttcattggat taaagaaagc
     6721 atttaatgcg ttaatcatgg ctttttgatt gccattgtgt aaataatttg ttgttaaggt
     6781 ataaaaatca ttttcactat tccaatcttt ggctaaccaa actaaatgac gatcagtaaa
     6841 agcaagttca gttaaaccac ttaatggtga atcctgagta ttctcgataa cgccctcaac
     6901 agcacacggt gcaccatcta catcataata tttctgcatc gcttcaggtg ttgtcatata
     6961 ttctacaaaa cgtttggctt cttctttatg ttgagaagtt gcagaaactg ataaggctag
     7021 gtctcctgcg ccaatcgtta gactttgacc tgcttcatct gctggaaaag gaaaagttcc
     7081 tacttcaaaa tctggatttt gtgaatgaat catcggcatt gcccatgaac cattcggcat
     7141 aatcaacgca tcaccttttg taaaagtaac tactgtatcg ttataaccgg caccttgcca
     7201 gttgttttgc aaagcttttg gttctcttaa caaatctaat cgattaaaat ctttttgcat
     7261 ttctttgctt tcagcattaa ttccattaac ttctgagaaa cgccaaacat tgttcgcttc
     7321 ctcttcacca ccagcagcag tcgctatagc tagttggtga taaccattta aagtccagcc
     7381 ttctgctcct gcaatcgcaa acggtacttt gttttgatct ttaatctcag ctactatttt
     7441 ttcaaattct gcccaagttt ctggtgcttg aatccccatt tcctcaaaag cagttttgtt
     7501 atagtaaaaa ccatagacat tagcggttaa tggtatgcta tatacacgat cctcaatagc
     7561 aaaacgttgt gcatatccat ttttgatatt ctctaaatac ggttcttcgg ttaagtcctc
     7621 taaataacca gctttcgccc attctttcaa ttctatactt tgaggataaa gattaatgac
     7681 atctggtaca tcgcctgcaa gcatacgtgt tttaattact tcaccagcat tcggtacatc
     7741 cacaactttt acatgaatat cagggttttt tgcttcaaaa tctttagcaa tctcctgaat
     7801 cgtttgagtc atttcttttt tttgattaaa aaactctatt tctaccttac cgcttgcatc
     7861 ttcctgattt tttccacaag cagtcaaaga gatcgttgct cctagtgcta tacaagctaa
     7921 aatgattttt ttagaccact tcattttatg ctcctcctta tacaatttga atgcgttttc
     7981 ttttttgtta acactaatgt aactgtttac taagtgtttt gtcaatattt ttactaaatt
     8041 taactaagaa aagagtaagg aattgaaaat tccttactct ttcagcttct ttttaattat
     8101 attcaggtaa atagtcccca tgtgcttcga ttaaatcatc cactaaattt ttgatatctg
     8161 ttaaagacaa ttcagatgat agatgtggat ctaaatacgc tgcttgataa atttttgatt
     8221 tatctagtgt taaagcggct tctactgtca gttcttgaac attgatattc gtccgattta
     8281 aagcagctaa ttgtgttggc aattcaccga cataggttgg ttgcacgcca tttttatcga
     8341 ctaaacaagg tacttccaca caaacatctg tcggtaagtt tgtgattaag cctttattta
     8401 agacatttcc tgcaatcact gacggagttc ctgtcgtaat agaatccatg atatatgaag
     8461 catattcacg gcttctcgta tgtgttaact ccccattatt tacaatgtcc tctcgcatgc
     8521 tttcccaatc attaatttgt tgttcacaac gacgtaagta ttcatccaac ggaatcccta
     8581 attcgtcaat caattctggg tattttgatt taataaagta tggatggtat tctgcattgt
     8641 attcacttga ttctgtaata tagtaaccaa aacgtttcaa caattcaaaa cgtacagaat
     8701 ctggatgtgg atttggtttt tctgcagcta actgacgaat tgttggatag aaatcttccc
     8761 cattttttgt aatttccaac aagaatgcca tatgattaat gcctgcaatt ttccattgaa
     8821 agtcatttaa gtcatattgg tcggctattc ctaaatgctc aaacaattct ggtacgcata
     8881 cttgaacgct atgacataaa ccaacagtct taatactcgt cgcttttaaa acacccatgg
     8941 ttaacatcgc catcggattt gtatagttca ataaccaagc atttggacaa acttcttcca
     9001 tttctttagc aaagtctaac attactggta gtgtccgtag ggttctaaaa atcccaccaa
     9061 tccccatggt gtcaccaata gtttgtcgca acccatattt tttaggaata tcaaaatcaa
     9121 tcactgttga aggtttataa ccacccactt gaatagcatt gataacaaaa tcagcctctc
     9181 ttaacgcttc tttgcgatca taataagaaa caatatcagc acgattttca ttcgaatttt
     9241 tattaatatt tttaagcatt aaatttgatt cttttagtcg tttttcatca atatcgtaca
     9301 atgcaatatg tgcatcttgt agacttggag ttaacatcgc atcgcctaat acgttttttg
     9361 caaaaatagt acttcctgca ccaataaatg taatttttgt catgtactta ctctccaatc
     9421 actaattaat agttttataa tttaccacct gttgtagcaa ttccttcaat gaattttctt
     9481 tggaaaacga tgaatatcac aactattggc caaattgcta ataacgaacc tgccattaat
     9541 tgtgggaagt tcgtggcata ttgtccttgt aagtttgcta atcccggagc taaaggcatc
     9601 ttatcaattg aagtattgac aatcaatggc cacattaaat ctttaaatgc aaacaacgct
     9661 gtgaatattc caagtgcaac caatcctgat ttagcaagcg gcaacataat tttgaaaaag
     9721 atcgtccaaa tattggcacc ttctaaatag gccgcttcct ccaattcatc aggtaaacct
     9781 aagaaaaatt gtcttaataa aaacgtacca aacgcactaa ctacccctgg taatattaat
     9841 gctggaattg tatttaaaat acccaatttt tgtgctaata aatactgtgg agtaataaaa
     9901 atggaaccgg gaatcatcat ttgaataagt actaagctga aaaggatatt tttccctgga
     9961 aaacttagac gcgcacaagc atatgcagcc attgctgaaa ataaaaccga gccaatcact
    10021 cgtccaacaa ccattaaaaa tgtattgata taaaaagcca tgaatggtaa taaatcaagt
    10081 acttgttgat agttacttag ctgaaatttc tcagggaaaa ttgtaggtgg aatttgggta
    10141 gattcaacca acgttttccc tgaagttaaa atcatccaaa taaaaggtaa tgccattgca
    10201 ataccaccta aaataagcat gatatggatt ggcgcatttg aaaaaaattt atttttcata
    10261 atttgatagc ctccttaata gtttacccac tttttctgca acttcatttg aattgcagta
    10321 ataattaata taatcactaa aaggaacata acgattgccg aaccatatcc cttgtcattt
    10381 aaaataaatg aatgtttata gaataaatag gctaatgatt gtgatttaaa catggcggta
    10441 ctattaggag taatcatcat aaaaataaca tcaaatactt ggaaagcatt gataactgtt
    10501 gttacaacta caaaaaataa tgttggtgta actaatggta acgtaatgtt gaaaaactgc
    10561 tggattggac tagcaccatc aatttccgct gcttcataat aatctttagg aatttcttat
    10621 aagccggcta gtaataacac catgttataa ccaatagcac tccaaactcc tacaatgata
    10681 atagaataaa tcgcaatcga tggatccgtt aaccatgctg gtccatcgag acctatttta
    10741 gccaacatgt tattaatcag tccataatca ttattgaaca tccagcgcca aatcattgcg
    10801 actgcggatg gtgcagcaac gactggtaga aaataaattg tccgataaat cgacaatcct
    10861 ttaacttttc tattcattaa aactgccacc aacaaggata atacaacaat actaggtatc
    10921 gaaaccactg cataaattaa tgtgtttaat aaagactgaa aaacttcacc gtctgcaaac
    10981 attttttggt aatttcttaa tccgacccat tcagtatcac cgaaccctac cgttttttga
    11041 aaacttaaaa ttgctgtttg aattgctggc catatattta ataaaattaa tcctaatgcc
    11101 gttggaaaaa taaaaatata gccccatatc acttgatctt tacgatattt tccaaaaatt
    11161 cgatttttca taatatcccc ctttttggta ttcatccatt aatataaaga cgaaatagaa
    11221 tcctttcgcc tgtatattgt ataattaact aatttaattt ccgctcaata tcgcattgac
    11281 ttctttttca atttgagcgg ttccatcttc aacagttact tcaccagaca gaatacggtt
    11341 aataatttga acttctgctt gttcagctcg taatccatct ttattgtatg gacgagggac
    11401 tccatactcc aacatttttc caaaagtact caaatcaatt gtatctgaag aagcagccca
    11461 ctcgtcttca aagcctttta gtgctgggat tgatgccccc gtttccgctt ggattttatt
    11521 ggcgtcctca ctagctaaaa atgccattag ttttttcact tcttctgggt attttgtatt
    11581 cgcactggca gcccaaccaa gtccatttgt aattgttgca ttaacttttc ccttaggtaa
    11641 cacggcagaa gcaacatttt caactagata gtcatttttc aaaaatcccg gtgcaaacca
    11701 gtttccttca atcatcattg ctaatctacc tgattgaaaa cgacttaact tatcaggatc
    11761 ttgtaattct gtaattgttg gtgaagcatg atcaacaagt gcaatatctc gataaaaagc
    11821 taaagcttct tgtgcttctg gggtattcaa acctgatttt gtattgtcat cggcaaatac
    11881 ttctccccca ttttgataaa taaatggcca gtagccagtc tctgttccaa tatgtgatgc
    11941 aaaaccatag atgccttttg attcatctgt taaatcaatt gcagcttgtc tgagtgtttc
    12001 ccatgtccat gtttcatctg gataatcgac acctgcatta tcaaataatt ctttgttgta
    12061 ccatagcccc actacagaag catctttagg gattgcgtat agttcgtctt caagcgtata
    12121 gaaatcagca attccttcag taaagtttcc catgtcaata tcattttctt tgataacatc
    12181 atcaagtccc atcaaagcat tctgtgacat atatttatag gattcgctag agtgcatggt
    12241 tactacatct ggcatattac ctcccgttgt tgcagcttct aatttaaccc aatattgatc
    12301 ccaaggcgtt gtctccattt ctactttgat atcaggattt tgtttttcaa attcatctaa
    12361 catttttctt agtcctggag cttggtcagt atctcaactc gcatagctta aagttacttg
    12421 cccgtcttta gaattactat ctcccccacc acatgctgcg agtaatgtcc cagttaaaac
    12481 agctatccct gttaaagcta tttttttgaa atgtttcatc ttaattcctc ctgttttgaa
    12541 tgcgttttct ttttgttaac aacaccataa ctgtttactt aatgttttgt cgagattttt
    12601 tactaaaaat attagtaaac aaaaaagtaa acaccctttt ttggatgttt acttttttaa
    12661 tcgatatttt tttcactttc tctaacaata atattattag aaacaatcac tttgatcgaa
    12721 acatttctaa cttcttttat tcgttcatta accatgcgaa ctgctagttt tccaatttct
    12781 tctgtattca cattcaccgt atttaatgca ggtgttaaaa attcagcaac ttcaatatta
    12841 tcaaaactca caatcgaaat atcttctgga atacgtagac catttttttg aagtgaacga
    12901 taaaccccca cagcaatagg atcatttcct accatgatag cagtcggcaa cttatcttta
    12961 gcatgttttt tcaaaagtaa atctgctgcc tgcattccgt caagtgtggt ccagccttct
    13021 aaataaattt gactatattt ttctaatcct ttttgtttca tccacgtctc ataggctatt
    13081 tttcgataat catcatctga cacgtgttta tttccttggc tatctaaacg aacacgtcct
    13141 cctccgataa acgcaatgtt tcggtgtcct tttttataca atcgatctaa atgctgattc
    13201 gttgcttttg ttaaatcagt ataaactgca tctacttcag cttcaatact agggtcatca
    13261 atcactacga cattcgaatt aattgctgat acttcttcaa tcacagaaga tagcacctgc
    13321 ccaataatca acacaccttg acattttagc aaatcgtctt gctccaacgg tgtttctgac
    13381 aaacgaataa ttttcttcaa attgactttg ctttgttctg cttcgacttg aatccctctg
    13441 cgaatcgttc gaaaataggg atcttccaac tcatctactt cagaaactgt cgttattaat
    13501 gcaatatttg ttaaattttt tgcaacggga ctttttttga cataatgata aaaattaacc
    13561 gattctgcaa tctttcgttt ggtttcatcg gttactgaaa gtgtcggatc ctcatttagc
    13621 acgcgcgata ctgttgcaat ggacacaccc gcccgttttg caacatccct aattcctacc
    13681 at
//
