LOCUS       AE006468                6952 bp    DNA     linear   BCT 24-JUL-2018
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2,
            complete genome.
ACCESSION   AE006468
VERSION     AE006468.2
DBLINK      BioProject: PRJNA241
            BioSample: SAMN02604315
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W.,
            Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F.,
            Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A.,
            Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W.,
            Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K.
  TITLE     Complete genome sequence of Salmonella enterica serovar Typhimurium
            LT2
  JOURNAL   Nature 413 (6858), 852-856 (2001)
   PUBMED   11677609
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   The Salmonella typhimurium Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2001) Genome Sequencing Center, Department of
            Genetics, Washington University School of Medicine, 4444 Forest Park
            Boulevard, St. Louis, MO 63108, USA
REFERENCE   3  (bases 1 to 6952)
  AUTHORS   McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R.,
            SanMiguel,P. and Csonka,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2016) Department of Microbiology and Molecular
            Genetics, University of California, Irvine, CA 92697, USA
  REMARK    Sequence update by submitter
COMMENT     On Jan 13, 2016 this sequence version replaced AE006468.1.
            Supported by NIH grant 5U 01 AI43283
            Coding sequences below are predicted from manually evaluated
            computer analysis, using similarity information and the programs;
            GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and
            GeneMark; http://opal.biology.gatech.edu/GeneMark/
            EC numbers were kindly provided by Junko Yabuzaki and the Kyoto
            Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/,
            and Pedro Romero and Peter Karp at EcoCyc;
            http://ecocyc.PangeaSystems.com/ecocyc/
            The analyses of ribosome binding sites and promoter binding sites
            were kindly provided by Heladia Salgado, Julio Collado-Vides and
            ReguonDB;
            http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset
            This sequence was finished as follows unless otherwise noted: all
            regions were double stranded, sequenced with an alternate
            chemistries or covered by high quality data (i.e., phred quality >=
            30); an attempt was made to resolve all sequencing problems, such
            as compressions and repeats; all regions were covered by sequence
            from more than one m13 subclone.
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. LT2"
                     /mol_type="genomic DNA"
                     /strain="LT2"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /culture_collection="ATCC:700720"
                     /culture_collection="SGSC:1412"
                     /type_material="type strain of Salmonella enterica"
                     /db_xref="taxon:99287"
                     /focus=""
     gene            complement(1..839)
                     /gene="nadE"
                     /locus_tag="STM1310"
     CDS             complement(1..828)
                     /gene="nadE"
                     /locus_tag="STM1310"
                     /EC_number="6.3.5.1"
                     /note="prefers NH3 over glutamine; similar to E. coli NAD
                     synthetase, prefers NH3 over glutamine (AAC74810.1); Blastp
                     hit to AAC74810.1 (275 aa), 89% identity in aa 1 - 275"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD synthetase"
                     /protein_id="AAL20235.1"
                     /translation="MTLQQEIIQALGAKPHINPEEEIRRSVDFLKAYLKTYPFLKSLVL
                     GISGGQDSTLAGKLSQMAIAELREETGDNALQFIAVRLPYGVQADEQDCQDAIAFIQPD
                     RVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTHGVVVGTDHA
                     AEAITGFFTKYGDGGTDINPLHRLNKRQGKQLLAALGCPEHLYKKVPTADLEDDRPSLP
                     DEAALGVTYDNIDDYLEGKTLDPAIAKTIEGWYVKTEHKRRLPITVFDDFWKR"
     regulatory      complement(834..839)
                     /regulatory_class="ribosome_binding_site"
                     /gene="nadE"
                     /locus_tag="STM1310"
                     /note="putative RBS for nadE; RegulonDB:STMS1H001558"
     gene            919..1388
                     /gene="osmE"
                     /locus_tag="STM1311"
     regulatory      919..927
                     /regulatory_class="minus_35_signal"
                     /gene="osmE"
                     /locus_tag="STM1311"
                     /note="putative -35_signal for osmE;
                     RegulonDB:STMLTH004482"
     regulatory      943..951
                     /regulatory_class="minus_10_signal"
                     /gene="osmE"
                     /locus_tag="STM1311"
                     /note="putative -10_signal for osmE;
                     RegulonDB:STMLTH004482"
     protein_bind    1026..1040
                     /gene="osmE"
                     /locus_tag="STM1311"
                     /note="putative binding site for IHF, RegulonDB:
                     STMS1H000219"
                     /bound_moiety="IHF"
     regulatory      1036..1041
                     /regulatory_class="ribosome_binding_site"
                     /gene="osmE"
                     /locus_tag="STM1311"
                     /note="putative RBS for osmE; RegulonDB:STMS1H001559"
     CDS             1047..1388
                     /gene="osmE"
                     /locus_tag="STM1311"
                     /note="similar to E. coli activator of ntrL gene
                     (AAC74809.1); Blastp hit to AAC74809.1 (112 aa), 96%
                     identity in aa 1 - 112"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator of ntrL gene"
                     /protein_id="AAL20236.1"
                     /translation="MNKNVAGILSAAAVMTMLAGCTAYDRTKDQFVEPVVKDVKKGMSR
                     AQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCAEY
                     DTDPQAPKQ"
     gene            1676..2009
                     /gene="celA"
                     /locus_tag="STM1312"
     regulatory      1676..1681
                     /regulatory_class="ribosome_binding_site"
                     /gene="celA"
                     /locus_tag="STM1312"
                     /note="putative RBS for celA; RegulonDB:STMS1H001560"
     CDS             1689..2009
                     /gene="celA"
                     /locus_tag="STM1312"
                     /note="similar to E. coli PEP-dependent phosphotransferase
                     enzyme IV for cellobiose, arbutin, and salicin
                     (AAC74808.1); Blastp hit to AAC74808.1 (106 aa), 96%
                     identity in aa 1 - 106"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|C4X744|4.A.3.2.7"
                     /protein_id="AAL20237.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLAG
                     EKGPAADVVLLGPQIAYMLPEIQRLLPGKPVEVIDSMLYGKVDGLGVLKAAVAAIKKAA
                     AN"
     gene            2078..3450
                     /gene="celB"
                     /locus_tag="STM1313"
     regulatory      2078..2083
                     /regulatory_class="ribosome_binding_site"
                     /gene="celB"
                     /locus_tag="STM1313"
                     /note="putative RBS for celB; RegulonDB:STMS1H001561"
     CDS             2092..3450
                     /gene="celB"
                     /locus_tag="STM1313"
                     /note="similar to E. coli PEP-dependent phosphotransferase
                     enzyme II for cellobiose, arbutin, and salicin
                     (AAC74807.1); Blastp hit to AAC74807.1 (452 aa), 94%
                     identity in aa 1 - 452"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P17334|4.A.3.2.1"
                     /protein_id="AAL20238.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMF
                     VLINNVFLSFGEGSFFYSMGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGM
                     ALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMF
                     TFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSIMGIISWALSNYGSNFHQIIMDTI
                     STPLASLGSVVGWAYVIFVPLLWFFGIHGSLALTALDSGIMTPWALENISIYQQYGSVD
                     AALEAGKTFHIWAKPMLDSYIFLGGSGATLGLIIAIFLASRRADYRQVAKLALPSGIFQ
                     INEPILFGLPIIMNPVMFIPFILVQPILAAITLVAYYLGIIPPITNIAPWTMPTGLGAF
                     FNTNGSVAALLVALFNLAVATLIYLPFVVVANKAQNAIEQEESEEDIANALKF"
     gene            3490..3848
                     /gene="celC"
                     /locus_tag="STM1314"
     regulatory      3490..3495
                     /regulatory_class="ribosome_binding_site"
                     /gene="celC"
                     /locus_tag="STM1314"
                     /note="putative RBS for celC; RegulonDB:STMS1H001562"
     CDS             3501..3848
                     /gene="celC"
                     /locus_tag="STM1314"
                     /note="similar to E. coli PEP-dependent phosphotransferase
                     enzyme III for cellobiose, arbutin, and salicin
                     (AAC74806.1); Blastp hit to AAC74806.1 (116 aa), 87%
                     identity in aa 1 - 115"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P69791|4.A.3.2.1"
                     /protein_id="AAL20239.1"
                     /translation="MFDLENVVDNQTAAEELEEVVMGLIINSGQARSLAYGALRKAKEG
                     DFEAAKAMMDQSRIALNEAHLVQTKLIEGDQGEGKMKVSLVLVHAQDHLMTSMLARELI
                     AELIELHEKLK"
     gene            3849..4701
                     /gene="celD"
                     /locus_tag="STM1315"
     regulatory      3849..3854
                     /regulatory_class="ribosome_binding_site"
                     /gene="celD"
                     /locus_tag="STM1315"
                     /note="putative RBS for celD; RegulonDB:STMS1H001563"
     CDS             3859..4701
                     /gene="celD"
                     /locus_tag="STM1315"
                     /note="AraC/XylS family; similar to E. coli negative
                     transcriptional regulator of cel operon (AAC74805.1);
                     Blastp hit to AAC74805.1 (280 aa), 85% identity in aa 1 -
                     280"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMI
                     LY|0035607"
                     /protein_id="AAL20240.1"
                     /translation="MMQLQVNATEIKTVYEQQLFNGKNFHVFIYNKTESVTGLHQHDYY
                     EFTLVLTGRYYQEINGKRVLLERGDFVFIPVGSNHQSFYEFGATRILNVGISKRFFEQH
                     YLPLLPFCFVASQVYRANSTFLTYIETVIASLNFRGNGLDEFIEVVTFYIINRLRHYRE
                     EQVYDDIPQWLKATVEIMHDKTQFGEHALENMVRLSAKSQEYLTRATRRYYSKTPMQII
                     NEIRINFAKKQLEMTNYSVTDIAYEAGYSSPSLFIKTFKKMTSFTPNSYRKRLTEINE"
     gene            4814..6181
                     /gene="celF"
                     /locus_tag="STM1316"
     regulatory      4814..4819
                     /regulatory_class="ribosome_binding_site"
                     /gene="celF"
                     /locus_tag="STM1316"
                     /note="putative RBS for celF; RegulonDB:STMS1H001564"
     CDS             4826..6181
                     /gene="celF"
                     /locus_tag="STM1316"
                     /EC_number="3.2.1.86"
                     /note="similar to E. coli phospho-beta-glucosidase; cryptic
                     (AAC74804.1); Blastp hit to AAC74804.1 (450 aa), 90%
                     identity in aa 1 - 450; cellobiose-6-phosphate hydrolase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH4"
                     /protein_id="AAL20241.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGK
                     EKLGIIYDLCQRMIDKAGVPLKLYKTLDRREALKGANFVTTQLRVGQLKARELDERIPL
                     SHGYLGQETNGAGGLFKGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVYRHT
                     NFKKFIGVCNIPVGMKMFIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELL
                     DGVASGQLKASTVKNIFDLPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGA
                     RAQVVQKVEKQLFDLYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQTEHYVNI
                     PHHGHVENIPADWAVEMTCILGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALS
                     GNFNDVLLALNLSPLVHSDRDAEVLARELILAHEKWLPNFAACIEALKGKHH"
     gene            6184..6952
                     /gene="celG"
                     /locus_tag="STM1317"
     regulatory      6184..6189
                     /regulatory_class="ribosome_binding_site"
                     /gene="celG"
                     /locus_tag="STM1317"
                     /note="putative RBS for celG; RegulonDB:STMS1H001565"
     CDS             6194..6952
                     /gene="celG"
                     /locus_tag="STM1317"
                     /note="similar to E. coli orf, hypothetical protein
                     (AAC74803.1); Blastp hit to AAC74803.1 (249 aa), 76%
                     identity in aa 1 - 249"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glucosidase"
                     /protein_id="AAL20242.1"
                     /translation="MERVLIVNADDFGLSKGQNYGIVEAYRNGVVTSTTALVNGEAIDH
                     AAQLSRELPALGVGMHFVLTLGKPVSEMPGLTRDGLLGKWIWQMAEEDTLPLDEIAHEL
                     ACQYQRFIDVFGSEPTHLDSHHHVHMFPQIFPIVARFAAQRGIALRIDRQTVLNADDLP
                     SDLRSTQGFSSEFYGEEITEACFLRILDASAHRGEASLEVMCHPAFVDNIIRQSAYCYP
                     RLTELEVLTSASLKAAIAERGYRPGSFLDI"
ORIGIN
        1 ttaccttttc caaaagtcgt cgaataccgt aatcggtaaa cggcgcttat gttcagtttt
       61 tacataccag ccttcgatag ttttagcgat ggcggggtcc agcgttttgc cttccaggta
      121 atcgtcaata ttgtcatacg ttacgcccag cgcggcttca tcaggcagtg aagggcggtc
      181 gtcttccaaa tcggcggtgg ggactttttt atacagatgc tcaggacagc ccagcgctgc
      241 gagaagctgt ttgccctggc gtttgttaag gcgatgaagc gggttaatat cggtaccgcc
      301 gtcgccatat ttggtgaaga acccggttat cgcttccgcc gcatggtcgg tgcccaccac
      361 gacgccgtga gtcataccgg cgatgctata ttgcgctttc attcgctcac gcgctttttc
      421 attgccgcga acaaaatcgc tcagctcaat tcccgcttcg cgcagcgcct gttcgctggc
      481 gaggacggcg cctttaatat tgacggtgag tactctgtcc ggctgaataa aagcaatggc
      541 gtcctggcaa tcctgttcat cagcctgtac gccataaggc agacgaacgg cgataaactg
      601 cagcgcatta tcaccggtct cttctcgcaa ttcggcgata gccatctggc tgagtttccc
      661 ggccagcgtc gaatcctgcc cgccgctgat gcctaacacc agcgatttca aaaagggata
      721 ggttttcagg tacgctttaa gaaaatccac gctgcggcga atttcttctt cagggttgat
      781 atgcggtttc gcgccaagcg cctggattat ctcttgctgc agagtcattt aacccccttc
      841 gacattaaaa aaatgaatct ttaccctgtt aaagctaacc cttcgccgct gaaacaacaa
      901 ggtttggcgg gctaagcggt gtttaaaaca acatgtcgct gcgttagaga attttccgaa
      961 agactttttg tggcgaggcg cggcttgaaa aatgttctga tgtattccag gcttacataa
     1021 cacgctgata aacaagagga cggaaaatga acaagaacgt agcaggaatt ttaagcgcag
     1081 cggcggtaat gactatgctg gcaggttgta cggcgtatga tcgtacaaaa gaccaatttg
     1141 ttgagcctgt cgttaaagat gttaaaaaag gaatgagcag ggcgcaagtt gcgcagattg
     1201 cgggcaaacc gtcatctgaa gtgagtatga ttcatgcgcg cggtacctgt cagacctaca
     1261 tcctgggtca acgtgatggt aaagcggaaa cctactttgt tgcgctggat gataccgggc
     1321 acgtgattaa ctccggctac cagacctgtg cggagtacga cactgaccca caggcgccga
     1381 agcagtaaca actgtacatt gcctgaacat tcaaggaaac cggcctgcga gccggttttt
     1441 ttgtgcctgc cataacctta tttattatcg cgaattattt gcccgaaatg tgaggggggt
     1501 cataacgcca ggtcaatgag agacaattta gtgggtcaag gaaataccat ccggtggtcc
     1561 gatcccgtat actcatttca gccacctaaa aaagtaaatc cggttacgca agtcgcccag
     1621 agtatggatg caggttgagg cttgcggcgt atctggttga cagataatcg cacatgagga
     1681 aggtttttat ggaaaagaaa cacatttatc tgttttgttc tgcgggcatg tcaacatcgt
     1741 tactggtatc aaaaatgcgc gctcaggctg aaaaatatga agtgccggtg attattgaag
     1801 cattccctga aaccctggcc ggtgaaaaag gcccggcagc ggacgtcgtg ttattaggcc
     1861 cgcaaattgc ttatatgctc cccgaaattc agcgtttatt acctggtaaa ccggtagaag
     1921 tcattgattc aatgttgtac ggcaaggtag atggcttagg cgtgcttaaa gctgcggtag
     1981 cggcaattaa aaaagccgcc gcgaattaat tattttttaa tttttcccgt caaagagtta
     2041 tttcatacac taaccgcaat attttattgc ggtatttaag ggtatttttc tatgagtaac
     2101 gttattgctt cacttgaaaa ggtactcctt ccttttgctg taaaaatagg aaagcagcca
     2161 cacgttaatg caatcaagaa cggctttatt cgtttaatgc cattaaccct tgcgggggcg
     2221 atgtttgtat taattaataa cgttttcctg agctttgggg aggggtcgtt tttttattcc
     2281 atgggcattc ggcttgatgc ctcaaccatt gaaacactta atggattgaa aggcatcggc
     2341 gggaacgtct ataacggcac attgggcatt atgtcgctca tggcgccatt ttttatcggc
     2401 atggcgctgg cggaagagcg caaggttgat gcgctggccg ccggattgct ttccgtggcg
     2461 gcatttatga ccgtaacgcc gtatagtgtc ggcgaggcct atgccgtagg cgctaactgg
     2521 ctgggtggtg caaatattat ctccggtatt attattggcc tggtggtcgc ggagatgttt
     2581 acatttattg tccggcgcaa ctgggtgatt aagctaccgg acagcgtacc ggcttcggtg
     2641 tctcgttcat tctcagcatt aattcccggc tttattattc tctctattat ggggattatt
     2701 tcctgggcgc tgtctaatta cggttctaac ttccatcaga ttattatgga cactatctct
     2761 acgccgctgg catcgctggg tagcgtggta gggtgggcat atgtcatttt tgtaccgctg
     2821 ctgtggttct ttggtattca tggttcgctg gcgctgaccg cgctggacag cggcatcatg
     2881 acgccctggg cgctggaaaa catctctatt taccagcagt atggctccgt cgatgcggcg
     2941 ctggaagccg gtaaaacgtt ccatatctgg gcgaaaccga tgctggattc ttatatcttc
     3001 ctcggtggta gcggcgcaac gctgggtctg atcatcgcta tcttcctggc atcccgtcgc
     3061 gcggactatc gccaggtggc aaaactggcg ctgccgtcag gtatcttcca gattaacgaa
     3121 cccatcctgt ttggcctgcc gattattatg aacccggtga tgtttatccc cttcattctg
     3181 gttcagccga ttctggcggc gattacgctg gtggcttact atttgggtat tattccgccg
     3241 attaccaata ttgcgccgtg gaccatgcca accgggttgg gggcgttctt taacaccaac
     3301 ggcagtgtcg ccgcgttgct ggttgcgcta tttaacctgg cggtcgctac cctgatttat
     3361 ctccccttcg tggtggtggc taacaaagcg cagaacgcca tcgagcagga agaaagcgaa
     3421 gaagatatcg ctaacgcact gaaattctaa actccgcgcc ggcatgggat gccatgccgg
     3481 gctgaatcag aggaaagact atgtttgatc ttgaaaatgt tgtagataac cagaccgcgg
     3541 ctgaggaact cgaagaagtc gttatggggc ttatcatcaa ttctggtcag gcgcgcagcc
     3601 tggcatatgg cgcgctcaga aaagcgaaag aaggcgattt tgaggcggcg aaagcgatga
     3661 tggaccagtc gcgcatagcg ctcaatgaag cgcatcttgt gcagacaaaa ctcatcgaag
     3721 gcgatcaggg cgaaggcaaa atgaaagtca gtctggtcct ggttcacgct caggatcatt
     3781 tgatgacatc aatgctggct cgtgaactga tcgccgagtt gattgagctc catgagaaac
     3841 tgaaataaca gggggcggat gatgcagcta caggttaacg caacggaaat caaaacggtt
     3901 tatgagcagc agctcttcaa tggcaaaaat ttccatgtgt ttatctataa caagacggaa
     3961 agcgtcaccg ggctgcatca gcacgactac tatgaattta ccctggtatt aaccggacgt
     4021 tattaccagg agattaacgg gaagcgtgtg ctgctggaac gtggagattt tgtttttatc
     4081 ccggtggggt cgaatcacca aagtttctat gagtttggcg caacgcgtat tctgaacgta
     4141 ggaattagta aacgtttttt tgaacagcat tatcttccgt tactgccgtt ttgttttgtg
     4201 gcatcgcagg tatacagggc gaatagcact ttccttacct atattgaaac ggtgattgcg
     4261 tcgttgaatt ttcgcggcaa tgggctcgac gaatttatcg aagtggtgac attttatatt
     4321 ataaaccgct tacgtcatta ccgcgaagag caggtttatg atgatattcc acaatggctg
     4381 aaagcaaccg tggagataat gcatgataaa acgcagtttg gcgaacatgc gctggaaaat
     4441 atggtgcgcc tgtcggcaaa atcccaggag tatctgacgc gcgccacccg acgttattac
     4501 agcaaaacgc cgatgcaaat cattaatgag attcgcatta attttgccaa gaaacagttg
     4561 gaaatgacca actattcggt tacggatatt gcttatgaag ccgggtatag cagtccaagt
     4621 ctgtttatta aaacgtttaa gaagatgacc tcatttacgc cgaatagtta tcggaagcga
     4681 ttgacggaaa ttaatgagta acggtttgca ttgctcgatt aattacattt cacgcttgcc
     4741 ccaatagcgc ggggaacgtc acgctatttc ttacgagcgg gcatagcgta atacatatca
     4801 gtgttataca ctgaagggag atattatgag ccagaaatta aaagtcgtca ctattggtgg
     4861 cgggagcagc tacacccctg aattacttga aggctttatt aagcgctacc atgaattacc
     4921 tgtcaccgaa ttatggctgg ttgacgttga agacgggaaa gagaagctgg gcattattta
     4981 tgatctctgc cagcgaatga ttgataaagc aggcgttccg ctaaaattgt ataaaacgct
     5041 ggatcgccgg gaagcgctga aaggcgctaa ttttgtcact acccagctac gcgttggtca
     5101 actcaaagcc cgtgagctgg acgagcgtat cccgcttagc cacggctatc tggggcaaga
     5161 aaccaacggc gctggcggtt tatttaaagg gttgcgtacc attccggtta tttttgacat
     5221 cattaaggat gttgaagaat tatgtccgaa tgcgtgggtc attaacttta ctaacccggc
     5281 ggggatggtg acggaagcgg tttatcgtca taccaacttt aaaaaattca tcggcgtatg
     5341 taatattcct gtcggcatga aaatgtttat tcatgacgtg ctggcgctga atgagaatga
     5401 cgatctttcc attgacctgt ttggtctaaa ccatatggtc tttattaaag atgtgctggt
     5461 gaatggcacc tcacggttcg cagaattact ggatggcgtg gcgtccggtc agttgaaagc
     5521 gtcaaccgta aaaaatatct ttgatctgcc gtttagtgaa ggattgattc gctcgctgaa
     5581 catgctgccg tgctcttatt tgttgtatta cttcaagcaa aaagagatgt tggcgattga
     5641 aatgggcgaa tattacaaag gcggcgcgcg cgctcaggtc gtacagaaag tggagaaaca
     5701 actcttcgac ttgtacaaaa atcctgagct aaacgtgaag ccgaaagagc ttgagcaacg
     5761 cggcggcgct tattattccg atgccgcttg tgaagtcatt aacgctattt ataatgacaa
     5821 gcagactgag cattacgtta atattccaca tcatgggcat gtcgagaata tcccggcgga
     5881 ctgggcggtg gaaatgacct gcattctggg acgcaatggc gcgacgccgc acccgcgtat
     5941 cacccgtttt gacgaaaaag tgctggggct tatccacact attaaaggat ttgaggtcgc
     6001 ggccagcaat gcggcgctga gcggaaactt taatgatgtt ctgctggcgc ttaacctgag
     6061 tccgctggtg cattccgacc gcgacgcaga agtcctggcg cgtgaactca ttctggcgca
     6121 tgaaaaatgg ctgcctaatt ttgccgcttg catcgaagcg cttaaaggta agcaccactg
     6181 acagaggtag gctatggaac gcgtgttgat tgtgaatgcc gatgattttg gcctgagtaa
     6241 agggcaaaat tacggcattg ttgaagcata tcgcaacggc gttgtgacct ccacgacggc
     6301 gctggtcaat ggcgaggcta ttgaccatgc ggcgcagctt agccgcgagc tgcctgcgct
     6361 tggggtggga atgcattttg ttttgacgtt aggcaaaccg gtgtcagaga tgccgggcct
     6421 gacgcgagac ggcctgttgg ggaaatggat ctggcaaatg gcggaggagg atacgcttcc
     6481 gctggatgaa attgcgcatg aactggcgtg tcagtatcag cgtttcatcg acgtatttgg
     6541 cagcgagcca acgcatctcg acagtcacca tcatgtgcat atgttcccgc aaattttccc
     6601 catcgtcgcc cgctttgcgg cgcagcgcgg gattgcgtta cgtattgacc gccagacggt
     6661 gttgaacgcc gacgatctgc cgtcggattt gcgcagcact caaggattta gtagtgaatt
     6721 ttacggcgag gagatcaccg aagcgtgctt tttacgcatt ctggatgcct cggcgcatcg
     6781 aggagaggcc tcgctggagg tgatgtgtca tccggcgttt gtggacaaca ttattcgcca
     6841 gagcgcctat tgttaccccc gcttaactga actggaggtg ttgacgtctg cgtcactcaa
     6901 ggcagcgatt gcggaacgtg gatatcgtcc tggcagtttt cttgatattt ag
//
