LOCUS       AF508972                4432 bp    DNA     linear   BCT 24-JUL-2016
DEFINITION  Corynebacterium glutamicum strain R probable substrate-binding
            component of iron ABC transporter gene, partial cds; and
            beta-glucoside catabolism operon gene, complete sequence.
ACCESSION   AF508972
VERSION     AF508972.1
KEYWORDS    .
SOURCE      Corynebacterium glutamicum
  ORGANISM  Corynebacterium glutamicum
            Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
            Corynebacterium.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Kotrba,P., Inui,M. and Yukawa,H.
  TITLE     A single V317A or V317M substitution in Enzyme II of a newly
            identified beta-glucoside phosphotransferase and utilization system
            of Corynebacterium glutamicum R extends its specificity towards
            cellobiose
  JOURNAL   Microbiology (Reading, Engl.) 149 (PT 6), 1569-1580 (2003)
   PUBMED   12777497
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Kotrba,P., Inui,M. and Yukawa,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2002) Molecular Microbiology and Genetics Lab,
            Research Institute of Innovative Technology for the Earth (RITE),
            9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Corynebacterium glutamicum"
                     /mol_type="genomic DNA"
                     /strain="R"
                     /db_xref="taxon:1718"
     gene            1..4432
                     /gene="beta-glucoside catabolism operon"
     regulatory      1..6
                     /regulatory_class="minus_35_signal"
                     /gene="beta-glucoside catabolism operon"
     regulatory      25..30
                     /regulatory_class="minus_10_signal"
                     /gene="beta-glucoside catabolism operon"
     misc_feature    74..100
                     /gene="beta-glucoside catabolism operon"
                     /note="ribonucleic antiterminator sequence"
     regulatory      95..141
                     /regulatory_class="terminator"
                     /gene="beta-glucoside catabolism operon"
                     /note="regulatory transcription terminator"
     gene            184..2053
                     /gene="bglF"
     regulatory      184..188
                     /regulatory_class="ribosome_binding_site"
                     /gene="bglF"
                     /note="putative"
     CDS             197..2053
                     /gene="bglF"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="function in beta-glucoside PTS"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8GGK3|4.A.1.2.5"
                     /protein_id="AAO15901.1"
                     /translation="MATKTDFSPLVADVVTALGGADNVRSVTHCATRLRFKIKDSDKAD
                     IAKAGKISGVITAINSGGQHQVVIGNDVPLAYQALMDVPGMRAKGIKDGDAEVDDDGGS
                     EDKNWLNKFIDLISALFSPIVWALAGIGLGKAGLTLATTVGILDAESSTHTIFNAMFDG
                     LFYFLPFFLAITAAKRFKVNQFIALAIVAALLHPTMEAMVGTEGVTFLGLPLTMMSYSS
                     SVIPVVVSVWLAGYLQRWLEKVLPGAVRNFFTPLIVVLVMVPLVLFTIGPVTIGAANAL
                     SNGIGFLFETVPWLAGGLMGAFWQVFVMFGLHWGLVPVMINDIATQGFSYMMAPLMAAI
                     FGQVGAALAVWIRSKNAEVKKVAGPGVLSGFFAGVTEPIIYGVNLPLKYPFYAGVVAGG
                     IGGIIIAVGGNAFDAFVFPSVMAFAATLSVGSFVAQLIGSSAALILGFVFTVVMFRKEQ
                     NVDKQETDTTPNVAAAASGTYVPLAEIPDKVFSSGAMGDGFGIVPENGEIVSPVEGQLI
                     AVQKSGHAYGIRGDNGIEVLVHVGIDTVKMAGEGFEILVARGDHVKVGQPLAKVDLNAV
                     AEAGFDPTTAVIVTNSKKLEGLKFTDVEGHVGAGDVVMRMEP"
     gene            2096..3523
                     /gene="bglA"
     regulatory      2096..2101
                     /regulatory_class="ribosome_binding_site"
                     /gene="bglA"
                     /note="putative"
     CDS             2114..3523
                     /gene="bglA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="function in utilization of beta-glucosides"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1"
                     /protein_id="AAO15902.1"
                     /translation="MTTFPQGFLWGGATAANQIEGAYNEGGKGLSIQDVMPGGAIKPST
                     EGPTEDNLKLKAIDFYNNYVEDIALFAEMGFKTFRISIAWSRIFPNGDEQEPNEEGLAF
                     YDRVFDELEKHGIEPLVTLSHYETPLGLARKYGGWRSRELIEFFAHYARTVFNRYKGRV
                     HYWLTFNEINAVLAMPFTAGGIEVPRSELSESELYQAIHHELVASALVTKIAHEIDPNN
                     QVGCMIIAVPRYGITPDPRDQLTAQQKSHEDFAFGDIHVRGEYPGYLLREFREKGITID
                     ITDEDREILKNTVDFVSFSYYMSVCEAHDPENYASGGGNIIGGVTNPTLEASEWGWQVD
                     PIGLRIVLNNYWERWQKPLFIVENGLGAKDVLIDGPSGPTVDDDYRIKYLNDHLVQVGE
                     AIEDGVNLLGYTTWGPIDLVSASTAQMSKRYGFIYVDRNDDGSGTLKRYKKKSFDWYRD
                     VIATNGGSLQA"
     gene            3548..4432
                     /gene="bglG"
     regulatory      3548..3552
                     /regulatory_class="ribosome_binding_site"
                     /gene="bglG"
                     /note="putative"
     CDS             3563..4432
                     /gene="bglG"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator protein"
                     /protein_id="AAO15903.1"
                     /translation="MKVLRVLNNNVVLAIKDGAEVVLTGWGVGFQKKPGEDVDQSKVTQ
                     VFVPENNRDADHMASLLAEVPLEYLDLSTELIASVSAELDSPVGSATVVALADHLQMAV
                     KRNGLEPELTSKPNPLSAEVHHLYPEETRLATEILERTNNWLAEKGTPPLPPAEVVAIS
                     LHLVNAGFRTEDLAETYVMTGVFEQLFEVIDSSFGITLDRQSVNAARFITHMRYFFVRV
                     HHDGQLNDGMSVLRNSLEISHPDSVACAERLSQILSLRLGAELSSDEQTYLALHVARLA
                     EDRGTTAD"
ORIGIN
        1 ttgcttttgc ttgcatgggt catacataat gagggcaatg cttttccaag aagactggtc
       61 gagggaaagc caaggattgt aacccgcaag gggcaagacc tgaaacgaaa gacgatgtgc
      121 atcgtctgag tttcaggtct tttgtcgttt tcggggtaga tgcacacgag ctttctgaac
      181 gaaaaggaca agaacaatgg caacgaaaac agacttcagc cccttggtgg ctgatgttgt
      241 caccgctttg ggaggcgctg acaacgtgcg cagtgtcacg cactgtgcca ccaggcttcg
      301 cttcaaaatc aaggattccg acaaggctga tatcgctaag gctggcaaaa tcagtggcgt
      361 cattactgcg atcaactctg gtggacagca tcaggttgtc atcggcaatg atgttccact
      421 ggcgtatcaa gcattgatgg atgtacccgg catgcgcgcc aaaggcatta aagatggcga
      481 tgcagaagtg gacgatgatg gcggttcaga ggataagaac tggctcaaca agttcatcga
      541 tctgatttct gcactgtttt caccgatcgt gtgggctttg gccggtatcg gtttgggtaa
      601 agctggtctt accctggcga ccactgtagg aattttggat gcagaatcaa gcacccacac
      661 cattttcaac gcgatgtttg atggcttgtt ttacttcttg ccattcttcc tcgcgataac
      721 ggctgctaag cgatttaaag ttaatcagtt cattgctctc gcaattgtgg ccgcattgct
      781 gcaccccacg atggaagcga tggtcggtac tgagggagtt accttcctgg ggctaccttt
      841 gaccatgatg tcttattctt catcagtgat ccctgtggtt gtgtcggtgt ggttggccgg
      901 atacctgcaa cgctggttgg aaaaggtact gcctggtgct gtccgtaact tcttcacgcc
      961 actgatcgtg gtgttggtga tggttccgtt ggtgctgttt accatcggcc cagtcaccat
     1021 cggtgcagca aatgcactgt ctaatggcat tgggttcctc tttgaaacgg ttccttggtt
     1081 ggccggtggc ctcatgggtg cattctggca ggtcttcgtg atgtttggtc tgcactgggg
     1141 actggtgcca gtgatgatca atgacattgc gacccaaggc ttctcctaca tgatggcgcc
     1201 actgatggct gcaattttcg gacaggtcgg tgcggctcta gcagtgtgga ttaggtccaa
     1261 gaacgctgaa gtgaaaaagg tagctggtcc tggtgtcctt tcaggattct tcgcaggtgt
     1321 tactgaacca atcatttacg gcgtgaacct tccactgaag taccccttct atgcgggtgt
     1381 cgtagctggt ggaattggtg gaatcatcat cgctgtgggt ggcaacgctt ttgatgcctt
     1441 cgtcttccca tcggtgatgg ctttcgcagc aacgctcagc gtgggtagct tcgtggcgca
     1501 actaattggt tcgtcagctg cgcttatctt gggatttgtg ttcactgtgg tgatgtttcg
     1561 aaaggaacaa aacgtcgata agcaagaaac cgacaccacc ccaaatgtgg ctgcggccgc
     1621 aagtggcacc tacgtgccgc tggcagagat tcctgacaag gtgttttcct ccggagccat
     1681 gggcgatgga tttggcatcg tgccggaaaa cggtgagatt gtttcaccgg tggaaggcca
     1741 gttgattgcg gtgcagaaat ctgggcatgc atatggcatc cgtggggata atggaattga
     1801 agttctggtg cacgtgggca ttgacaccgt caaaatggca ggtgagggat tcgaaatcct
     1861 cgtggcgcgt ggcgatcacg tcaaagttgg gcagccgctg gccaaggttg atttgaatgc
     1921 tgttgcggag gctggtttcg atccgaccac tgcggtgatc gtgactaact ccaagaaact
     1981 ggaaggcttg aaattcactg atgttgaggg acacgttggc gcaggagatg tagtgatgcg
     2041 tatggaacct taaaaggttt catcgccctc acccaccaca ccacacataa tttagaagga
     2101 gttctaaacc accatgacca cattcccgca aggtttcctc tggggcggcg cgaccgcagc
     2161 caaccaaatc gaaggtgcat acaacgaagg cggcaagggt ctgtcgatcc aggacgtcat
     2221 gccgggcggt gcgattaagc cgtcaacgga aggccccacc gaggacaacc ttaagctcaa
     2281 ggccattgat ttctacaaca actacgtcga agacatcgca ctcttcgcgg agatgggttt
     2341 caagactttc cgtatctcca tcgcttggtc ccgtattttc cccaacggtg atgagcagga
     2401 gccaaacgaa gagggcttgg cgttctacga ccgcgtattc gatgagctgg aaaagcacgg
     2461 tattgagcca ctagttactc ttagccacta cgaaactccg ctgggtttgg ctcgtaaata
     2521 tggtggatgg cgcagccgtg agctcattga attcttcgct cattacgccc gcactgtatt
     2581 taaccgatac aagggacgtg tgcattactg gctgaccttc aacgagatca atgctgttct
     2641 ggcgatgcct ttcaccgcgg gtggaattga agttccacgc tctgagctga gtgaatctga
     2701 gctctaccaa gccattcacc atgagttggt ggcgagcgcg ttggtcacca agattgccca
     2761 tgagatcgat ccgaacaacc aggtcggctg catgattatt gcagttccgc gttatggaat
     2821 cactcctgat ccacgcgatc agctcaccgc gcagcagaag tcgcatgagg attttgcttt
     2881 cggcgatatt cacgtccgcg gcgaataccc aggctacttg ctgcgtgaat tccgcgaaaa
     2941 gggcatcacc atcgacatca cggatgaaga tcgtgagatc ctcaagaaca ccgtcgactt
     3001 cgtgtccttc tcctattaca tgtcggtctg tgaagcccac gatccggaaa actacgcatc
     3061 tggtggcggc aacatcatcg gcggtgtcac caacccaacc ctcgaggcat ccgagtgggg
     3121 ctggcaagtt gacccaatcg gcctgcgcat cgtcctcaac aactactggg agcgctggca
     3181 aaaaccactg tttatcgtcg aaaacggact aggagcaaag gacgtgctta tcgacggacc
     3241 ctccggccca acagtcgatg acgactaccg catcaagtac ctcaacgatc acctcgtgca
     3301 ggtcggcgaa gcaatcgaag atggcgtcaa cctgctgggc tacaccactt ggggtcccat
     3361 tgacttggtc tctgcctcca cagctcaaat gtccaagcgc tatggtttca tttatgtaga
     3421 ccgcaacgat gatggctccg gcaccttgaa gcgctacaag aaaaagtcct tcgactggta
     3481 ccgcgatgtc atcgcaacca acggtggaag cctgcaggct taagaactgc cggtttaagt
     3541 gaaaaaggga ggtgtcacct cggtgaaagt gttgagagtg ctcaacaata atgttgtgct
     3601 tgccatcaaa gacggtgccg aggtggtgct gaccggatgg ggtgtgggtt tccagaagaa
     3661 gcctggtgaa gacgttgatc agagcaaggt cacgcaggtt ttcgttcctg aaaacaatcg
     3721 cgacgctgat catatggcca gtcttttggc cgaagtgcca ctggaatacc tcgacctatc
     3781 cactgaactg atcgcatcgg tatcggctga acttgattcc ccagtgggca gcgcaacggt
     3841 ggtggcgttg gcggatcacc ttcaaatggc ggtgaaacgt aatgggttgg aacccgagct
     3901 gacctccaaa cccaacccat taagcgcaga agtgcatcat ttgtatcctg aggaaactcg
     3961 tcttgcaacg gagatcctgg aacgcaccaa caattggctt gctgaaaaag ggacccctcc
     4021 gctgccacca gcggaagttg tagccatctc attacacctg gttaatgctg gtttccgcac
     4081 ggaagacctc gcagaaacct acgtgatgac tggcgttttc gagcagctct ttgaggtaat
     4141 cgattcctcg tttggcatca cccttgaccg acaatccgtc aacgccgcac ggtttatcac
     4201 ccacatgcgc tacttctttg ttcgcgttca ccatgatggc caactcaacg acggcatgtc
     4261 cgtgctgcgc aacagcctag aaatctcaca cccagactcg gtggcatgcg cggaaagact
     4321 cagccaaatc ctcagtctcc gattgggtgc cgagctttcc tccgacgagc aaacctacct
     4381 cgcgctccat gtcgcgaggt tggctgaaga tcgaggtact accgctgatt aa
//
