LOCUS       AB063629                7232 bp    DNA     linear   BCT 14-DEC-2007
DEFINITION  Pseudoalteromonas piscicida chiD, cbp1, chiA genes for chitinase D,
            chitin-binding protein 1, chitinase A, complete cds.
ACCESSION   AB063629
VERSION     AB063629.1
KEYWORDS    .
SOURCE      Pseudoalteromonas piscicida
  ORGANISM  Pseudoalteromonas piscicida
            Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
            Pseudoalteromonadaceae; Pseudoalteromonas.
REFERENCE   1
  AUTHORS   Tsujibo,H., Orikoshi,H., Tanno,H., Fujimoto,K., Miyamoto,K.,
            Imada,C., Okami,Y. and Inamori,Y.
  TITLE     Cloning, sequence, and expression of a chitinase gene from a marine
            bacterium, Altermonas sp. strain O-7
  JOURNAL   J. Bacteriol. 175 (1), 176-181 (1993)
   PUBMED   8416892
REFERENCE   2
  AUTHORS   Tsujibo,H., Orikoshi,H., Baba,N., Miyahara,M., Miyamoto,K.,
            Yasuda,M. and Inamori,Y.
  TITLE     Identification and characterization of the gene cluster involved in
            chitin degradation in a marine bacterium, Alteromonas sp. strain O-7
  JOURNAL   Appl. Environ. Microbiol. 68 (1), 263-270 (2002)
   PUBMED   11772635
REFERENCE   3  (bases 1 to 7232)
  AUTHORS   Tsujibo,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2001) Contact:Hiroshi Tsujibo Osaka University of
            Pharmaceutical Sciences, Department of Microbiology; 4-20-1
            Nasahara, Takatsuki, Osaka 569-1094, Japan
COMMENT     On Jan 14, 2002 this sequence version replaced D13762.1.
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Pseudoalteromonas piscicida"
                     /mol_type="genomic DNA"
                     /strain="O-7"
                     /db_xref="taxon:43662"
                     /note="originally submitted as 'Alteromonas sp. O-7'"
     gene            1..3150
                     /gene="chiD"
     CDS             1..3114
                     /gene="chiD"
                     /note="ChiD"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM5|GH18"
                     /protein_id="BAB79618.1"
                     /translation="MKQHVKTLKLSQMASILGAIFCANSYAYNCDGLPEWSQSSAYNGG
                     AQVQKSQQAFEAKWWTQADPVTHSGQWDDWKKLGDCDSVVTNTPPTVQSLLPADGSAFN
                     ENDSVAISVNATDSDGQVTQVEFFVGGTSVGIDTTATGDNFSFNWSATAGTHAISVVAT
                     DDKQAVSNTLITNIIVNTTGNQLPTANLSLGGGNNLVINQPVDIVLDGSDPDGSIAKLT
                     LLINDQVAYESTTAPAQHTWTPTQLGSVTLKLVVEDNTGATAESILNANVVETPPVVNS
                     CVPHGLYQTPNISPNYCDIYDENGREKMGTDHPRRVIGYFTSWRTGKNGQPSYLVDDIP
                     WDKITHINYAFAHVDANNKVSIGNPTAPNNAATNMTWPGVAGAEMDPSLPYTGHFNLLN
                     KFKKQHPDVKTLISVGGWAETGGYFDETGKRIASGGFYTMTTNADGSVNHAGIDAFVAS
                     SVEFIRKYNFDGVDIDYEYPSSMNDSGHPDDFAISNPRRAALNASYQVLMKKLREALDQ
                     AGQQDNTHYLLTIASPSSGYLLRGMETFQVTKYLDYVNIMSYDLHGAWNQHVGHNASLF
                     DTGEDSELKQWNVYGTKEFEGIGYLNTDWAVKYFRGSVSAGRINIGIPYYTRGFRDVTG
                     GTNGLWGQAALPNQADCPPGTGSGEKNKCGNGAVGIDNLWHDKNDVGLEVPAGSNPLWH
                     VKNLQNGVLGSYLSDYGLDPVNNPEHQISGTYARHYDSVAVAPWLWNATKNVFLSIEDE
                     ESMAAKVDYVINQELGGIMFWELAGDFDYDVSKNEYFMGSSLTSLAYNKFNQSGTPYSV
                     KQGNKTFVKPASAVDVTFVAKDFPAGDANYPIAPTFAFTNNSAIDLSGAKISFDVPVAT
                     SAIFKSNWNAQEKLGMAVDVNGSNAAGNNIGGFENEFHRFSITLVNEWGGTPKSFAPGE
                     TINAQVMYYMPISGPVNFVVEKDGNRYAFSSEYSDLPIATPGTGPTDPVTSCKGTPISQ
                     IVVYPNLPQGTHAAQGDLIVEGNAVYEAKWWSNKQPSVSGDYTKACNL"
     sig_peptide     1..81
     regulatory      3137..3150
                     /regulatory_class="terminator"
                     /gene="chiD"
     regulatory      3147..3150
                     /regulatory_class="ribosome_binding_site"
                     /gene="chiD"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     gene            3160..4609
                     /gene="cbp1"
     CDS             3160..4587
                     /gene="cbp1"
                     /note="Cbp1"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM5|AA10"
                     /protein_id="BAB79619.1"
                     /translation="MNSLHRKYALGLFGATSALISSAVSAHGFMDFPKARQAICQAQGG
                     YWWPDDGSNIPNLACRAAFLDSGHFQFVQEHEFSTNTADYNNLEAVKANIPNGTLCSAG
                     DPNKSGMSVVSPHWQRTVVEPNAQNKLAISFRATTPHNPSFWQFFLSKPNYDGDTSPLT
                     WNDLELINEFGNIDFVVDPEGKRFYKMEIDIPQGREGNAVLYTRWQRFDVAGEGFYNCS
                     DITITSTGTPPEWVSSGYFLKQGQDAKAGDSVWFRTFDQSGNELISHNLNVTSTNASTW
                     QAELALYLNSNYGNKVNIGVKQPDGSIAFDANALLANEVFLPHAAYTSNLSVVKGGDNT
                     APTVNPISNQSIAENSQATVHAHAFDDQNDPLTFIWSIPAPLSFSGEGDTISISTPEVT
                     ADQTFEAELNVTDGKASTTVSFSITVQDSTNNQYPAWNSTTTYVAGDRVTHQQKVYEAK
                     WWTQGEEPGASDVWKAI"
     sig_peptide     3160..3237
     regulatory      4593..4609
                     /regulatory_class="terminator"
                     /gene="cbp1"
     regulatory      4627..4631
                     /regulatory_class="ribosome_binding_site"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     gene            4640..7232
                     /gene="chiA"
     CDS             4640..7102
                     /gene="chiA"
                     /note="ChiA"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM5|GH18"
                     /protein_id="BAB79620.1"
                     /translation="MKLNKITSYIGFALLSGGALAAPSTPTLDWQPQQYSFVEVNVDGL
                     GSYKQLVKAKDVVDISIKWNAWSGSGGDNYKVYFDDLLVNQGSLPAGTKSGVVQFPYTK
                     SGRHQLYLELCEGTVCARSAGKEIVIADTDGAHLAPLPMNVDPNNRNNGTIPGRVTGAY
                     FVEWGIYGRNYDVTKIPAHNLSHILYGFIPICGPNESLKSIEIGNSWRALQTACADSQD
                     YEVVIHDPWAAVQKSMPGVDAKDPIRGVYSQLMALKQRYPDLKILPSVGGWTLSDPFHG
                     FTNKANRDTFVASVKQFLKTWKFYDGVDIDWEFPGGDGPNPDLGDPINDGPAYVALMQE
                     LRAMLDELEAETGRQYELTSAIGAGYDKIEDVDYQAAQQYMDYIFAMTYDFYGAWNNET
                     GHQTGIYCGSHLSTDECNGTGVDDNGVPRKGPAYTGDHAIQLLLQQGVQPSKLVMGVAM
                     YGRGWEGVLDANAAIPGNPMTAPGNGPLTGSTSEGVWEPGIMDYKAIAANAVGQGGSGV
                     NGYEVGYDEQAQAAYVWNRSNGKLITYDSPRSVIAKGQYANTHQLAGLFGWEIDADNGD
                     ILNAMYDGLTAGEIPNRAPTIGVSGPINVTSGQVVNVDAQASDLDNDPLTYSWVAAPGL
                     ALSANNTAAVAVTAPSVAQQTSYDLTVTVNDGALSTTKTIVVVVNPEGANAAPVVTPVS
                     DISVNEGASATVNVSATDPEGAALSYSWSVPAELSVANGSSATITAANVTADTTVPVTV
                     TVSDGVNAVDTTFNVTIKDGAEYPTWDRSTVYVGGDRVIHNSNVFEAKWWTQGEEPGTA
                     DVWKAVTN"
     sig_peptide     4640..4702
     regulatory      7212..7232
                     /regulatory_class="terminator"
                     /gene="chiA"
ORIGIN
        1 atgaaacaac atgtaaagac ccttaaatta tcgcaaatgg cttcgatcct cggagccatt
       61 ttctgcgcta actcatatgc ttataattgt gatggtttac ctgaatggtc acaaagttcg
      121 gcatataatg gtggtgctca agtacaaaaa tcgcaacaag cttttgaagc aaagtggtgg
      181 actcaggctg atccagttac ccattccgga caatgggatg attggaaaaa attgggcgac
      241 tgtgacagcg ttgtcaccaa cacaccacct acagtacaaa gcttactccc agctgacggc
      301 agcgctttta acgaaaatga cagcgttgcc atttctgtaa atgcaacaga tagcgatggc
      361 caagtaacgc aggtagagtt ttttgtaggt ggcacgtctg ttggtattga taccacagcg
      421 acaggtgata atttttcatt taactggtca gcaaccgctg gaacacacgc aatttcagta
      481 gttgcaacag atgacaagca agctgtgtcc aacacattaa taactaatat tatcgttaat
      541 acgacaggaa atcagttacc tacagcaaac ctttcgttag gcggaggtaa taatctagtt
      601 attaatcagc ctgtagacat cgttcttgat ggttcagatc cagacggctc aatcgcaaaa
      661 ctaacactgc ttattaacga ccaagtcgca tatgagagca caactgcacc ggcacagcac
      721 acttggacac caacgcaact gggctccgta acattaaagc ttgttgttga agataatacc
      781 ggtgcgacag cagagagtat actcaatgca aatgtggttg aaacaccgcc agtggtaaac
      841 agctgtgttc cgcatggtct ttatcagacc cccaacatat cgcctaacta ctgtgatatc
      901 tatgatgaaa atggccgtga aaaaatgggt actgatcacc ctagacgcgt catcggttac
      961 tttaccagtt ggagaacggg taagaatggt caaccgagct acttagttga tgatattcca
     1021 tgggataaga tcacccacat caattatgct ttcgcgcacg ttgatgccaa caacaaggtt
     1081 tcaataggca atccaactgc tcctaacaat gcagcgacga atatgacttg gccgggggtt
     1141 gctggtgcag agatggatcc atctcttcct tataccggtc attttaactt gctgaacaaa
     1201 tttaagaaac agcatcctga tgttaaaacc ttgatctcag tcggcggttg ggcggaaact
     1261 ggtggctact tcgacgaaac aggtaagcga attgccagcg gcggtttcta tacgatgacg
     1321 accaatgcag atggcagcgt gaatcacgca ggtattgatg cgtttgtagc aagttcagtt
     1381 gaatttatcc gtaaatacaa ctttgatggt gtcgatattg actatgaata cccatcttct
     1441 atgaatgact ctggtcaccc cgatgatttt gcgatatcaa acccgcgtcg agcagcattg
     1501 aatgcttctt atcaagtatt aatgaaaaaa ctgagagaag cattagacca agcaggtcaa
     1561 caagacaata ctcactattt attaacgatc gcatcaccat cttctggtta ccttctacgt
     1621 ggtatggaga cattccaagt cactaagtat ctagactacg tcaatattat gtcttatgac
     1681 ttacacggag cgtggaacca gcacgttggc cataacgcat cgctattcga caccggcgaa
     1741 gattccgagc ttaaacagtg gaatgtttac ggcaccaaag agtttgaggg aattggttac
     1801 cttaataccg attgggcagt caaatatttc agaggctctg tctctgcagg acgcatcaac
     1861 ataggtatcc cttattatac tcgcggcttt agagatgtaa caggtggtac aaatggtttg
     1921 tgggggcaag ctgcattacc taaccaagcc gattgtcctc cgggaactgg cagcggtgaa
     1981 aagaacaagt gcggtaatgg tgcagtgggt attgataacc tgtggcacga caagaacgat
     2041 gttggcttag aagtaccggc aggctctaac ccgctttggc atgttaaaaa ccttcaaaat
     2101 ggcgtgttag gaagttacct aagtgattat ggactagatc cagttaacaa cccagagcat
     2161 cagattagcg gtacttatgc acgccactat gattccgtag cggttgctcc ttggctttgg
     2221 aacgccacca agaatgtttt cttatccatt gaagatgaag agtcaatggc cgctaaagtc
     2281 gactatgtga tcaaccaaga actaggcgga atcatgttct gggagcttgc tggtgacttc
     2341 gattatgatg taagcaaaaa tgaatacttc atgggttcat cgctaacaag cttagcctac
     2401 aacaagttta accaaagtgg cacgccatac tccgttaaac aaggaaataa gacctttgta
     2461 aaaccagctt ctgcagttga tgtcacattc gttgctaaag atttcccagc tggagatgct
     2521 aactatccaa ttgcaccgac ttttgccttt accaataact cagcgataga tttgagtggc
     2581 gcaaaaattt cgtttgatgt tcctgttgca acatcggcaa ttttcaaatc taactggaat
     2641 gcacaagaaa agcttggcat ggcagtggac gtaaatggat cgaatgctgc gggtaataat
     2701 attggcggct tcgaaaacga attccaccga ttctcgatca cgcttgtgaa cgagtggggt
     2761 ggtacgccta agtcttttgc tcccggcgaa actatcaatg ctcaagtcat gtactacatg
     2821 cctatttctg gccccgttaa ctttgtggtt gagaaagacg gtaaccgcta tgcattttcc
     2881 agtgaatata gcgacctacc aatcgcaaca cctggtacag ggcctacaga tccggtaact
     2941 agctgtaaag gtacacctat cagtcaaatt gtggtttacc cgaacctacc acagggcacg
     3001 catgctgctc aaggtgattt aattgttgaa ggaaacgcgg tgtatgaagc gaaatggtgg
     3061 agcaataagc aaccatcagt tagcggcgat tataccaagg cttgcaactt gtaaataata
     3121 aatggggagc tttgctcctt ttataaaagg cgaataagca tgaatagttt acaccgtaaa
     3181 tatgcgcttg gtctttttgg cgcaacctca gctttgattt caagcgcagt ttcagctcat
     3241 ggttttatgg actttcccaa agcaaggcag gcgatctgtc aagcgcaagg aggttattgg
     3301 tggccggatg atggatcaaa tatcccaaac ctagcatgtc gagcggcttt tctagactca
     3361 ggccattttc agtttgtgca agagcatgaa ttttcgacca ataccgctga ctacaataat
     3421 ctagaggcag taaaagcgaa tatccccaat ggtacgcttt gctcagctgg tgacccaaac
     3481 aaaagtggca tgagtgttgt ctcaccacac tggcagcgta cagttgtaga gccaaacgct
     3541 caaaataaac ttgccattag ctttagggcg acaacacctc ataatcccag tttttggcag
     3601 ttcttcttgt caaagccaaa ctacgatggc gatacatctc ctctgacttg gaatgattta
     3661 gagcttatta atgaatttgg caatattgat ttcgttgtgg atcctgaagg taaacgcttc
     3721 tataaaatgg aaatagatat accgcaaggc cgtgagggca atgcagtttt atatacccgt
     3781 tggcagcggt ttgatgtcgc tggagaggga ttttacaatt gctctgatat cacaataacc
     3841 tcaacaggca cgccaccaga atgggtgagc agcggctatt tcttaaagca agggcaagat
     3901 gctaaagccg gtgatagcgt gtggtttagg acgtttgatc aatccgggaa tgaattaatt
     3961 agccataact tgaatgtaac ctccactaat gcatcaacat ggcaagctga gttggctctt
     4021 taccttaata gcaattacgg aaataaggtc aacattggtg ttaagcaacc ggacggttca
     4081 attgcgtttg atgcaaacgc cttattagca aacgaagtat ttttgccaca tgccgcttac
     4141 accagcaatc ttagcgttgt aaaagggggt gacaacaccg ctcctacggt aaatccaatt
     4201 tctaaccaaa gcatcgcaga aaatagccaa gcgacggtgc atgctcatgc gtttgacgac
     4261 caaaatgacc cactaacatt catttggagt attcctgcac cgcttagctt tagtggcgaa
     4321 ggtgacacga tttccatctc aacacctgaa gtgacggctg atcaaacctt tgaggctgaa
     4381 cttaatgtca ccgatggcaa agcaagcacg accgtaagtt tctcaattac cgttcaagat
     4441 agcaccaata accaataccc agcttggaat agtacaacta cctatgtcgc aggcgacaga
     4501 gtaacgcatc aacagaaagt gtatgaagct aagtggtgga cgcagggaga agagcccggc
     4561 gcatccgatg tttggaaagc aatttaaaga aaacccaaat taattgggta caacatgtaa
     4621 ctacaataag gttgaagata tgaaacttaa taaaataacc agctatatag gatttgcctt
     4681 actgagtggc ggggcacttg cagccccttc aacaccaaca ttagattggc agccacaaca
     4741 gtattcgttc gttgaagtta acgtagatgg gcttggctct tacaagcaac tggtaaaagc
     4801 caaagatgtt gtcgatatta gcatcaaatg gaatgcatgg agtggctctg gcggtgataa
     4861 ctataaagta tattttgatg atctcctcgt aaaccaaggc agcttgccag ctggtactaa
     4921 aagtggggta gtacaattcc cctacactaa atcgggtcgc catcagcttt atttagaact
     4981 gtgtgaaggt accgtatgtg caagaagcgc agggaaagaa atcgtcattg ccgacactga
     5041 cggtgcacat ctagcgccac ttcctatgaa tgtagatccg aacaaccgta ataacggtac
     5101 aataccaggc cgtgtaacgg gtgcatattt cgtcgaatgg ggcatatatg gccgtaatta
     5161 tgacgtaact aagatcccag ctcataactt atcgcacatt ttatatggct ttattccgat
     5221 ttgcggacct aacgaatcac tcaaatccat cgaaattggt aatagttgga gagcgcttca
     5281 aactgcatgt gctgactccc aagattatga agtcgtcatt cacgaccctt gggctgcagt
     5341 gcaaaaatct atgccagggg tggatgcaaa agatcctatt cgcggtgtat attctcaatt
     5401 aatggcgcta aaacaacgtt atccggacct taaaattctg ccttcagtcg gtggatggac
     5461 gctatctgac ccattccatg gctttacaaa caaagcaaac cgcgacactt tcgtggcgtc
     5521 tgtaaaacaa tttcttaaaa cgtggaagtt ttatgacggt gtagatatcg actgggaatt
     5581 cccaggtggt gatggtccaa acccagactt gggcgatcca attaacgacg gtccagcata
     5641 tgtggcgcta atgcaagaac ttcgagcaat gctggatgag ttagaggctg aaaccggacg
     5701 tcaatatgag ctcacttctg caataggtgc gggttatgac aagattgaag atgtagatta
     5761 ccaagctgcc caacagtata tggattatat ctttgcgatg acctatgact tctatggtgc
     5821 ttggaacaat gaaacgggtc atcaaaccgg tatctattgt ggctctcatc tcagcacaga
     5881 cgaatgtaac ggtacaggcg ttgacgacaa tggagtgcca cgtaaaggcc cagcatatac
     5941 tggcgaccat gcaattcagt tgttacttca acaaggtgtg cagccctcta agctcgttat
     6001 gggcgttgca atgtacggcc gcggctggga aggtgtgcta gatgcaaatg cagccattcc
     6061 aggtaatccg atgacggcgc cgggtaatgg cccattgaca ggttctacaa gtgaaggtgt
     6121 ttgggagccg ggcattatgg attacaaggc tattgcagca aacgctgtag gccaaggtgg
     6181 ttcaggcgtt aatggttatg aagtaggcta cgatgagcaa gcacaagcag catatgtttg
     6241 gaacagaagt aacggtaaac tcatcactta cgatagccca cgcagcgtta tcgcaaaagg
     6301 ccagtatgca aacactcatc aactagctgg tttatttggt tgggaaattg acgctgataa
     6361 tggcgacatt ctaaatgcaa tgtatgatgg tcttacagca ggtgaaatcc ctaatcgcgc
     6421 tcctactatc ggggtttctg gaccaatcaa tgtgacctca ggtcaggttg tgaacgtcga
     6481 tgcgcaagca agcgatttag acaatgatcc attaacctat tcatgggttg ctgcgcctgg
     6541 tttggctttg tctgctaaca acaccgcagc tgtggctgta actgctcctt cagtcgctca
     6601 acagacaagt tacgacttga ctgtaacggt caatgatggg gcgttatcaa caaccaaaac
     6661 aatcgttgtt gttgtaaacc cagaaggtgc aaatgcagcg cctgtcgtta caccggtttc
     6721 agatatttcg gttaacgaag gggcctcggc tactgtcaat gtgtcagcaa ctgaccctga
     6781 aggcgcagca cttagctata gctggagtgt accagccgag ctaagcgtag caaatggtag
     6841 ctcagcgact attactgctg caaatgtcac agccgacaca accgtcccgg taactgttac
     6901 cgtatctgac ggcgttaacg ctgttgacac aacatttaat gtcacgatta aagacggtgc
     6961 tgaatatcct acttgggatc gatcaacagt ttatgtcggt ggcgaccgag taattcataa
     7021 cagcaatgtc tttgaagcaa aatggtggac tcaaggtgaa gaacctggaa ccgcagatgt
     7081 atggaaggca gtaactaact aattcataac agcaatgtct ttgaagcaaa atggtggact
     7141 caaggtgaag aacctggaaa ccgcagatgt atggaaggca gtaactaact aatctaacca
     7201 tccccaatta atgagcttgc cagtcaagct ca
//
