LOCUS       AF441242                5900 bp    DNA     linear   BCT 06-JAN-2003
DEFINITION  Bifidobacterium lactis sucrose-utilizing gene cluster, complete
            sequence.
ACCESSION   AF441242
VERSION     AF441242.1
KEYWORDS    .
SOURCE      Bifidobacterium animalis subsp. lactis (Bifidobacterium lactis)
  ORGANISM  Bifidobacterium animalis subsp. lactis
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Trindade,M.I., Abratt,V.R. and Reid,S.J.
  TITLE     Induction of sucrose utilization genes from Bifidobacterium lactis
            by sucrose and raffinose
  JOURNAL   Appl. Environ. Microbiol. 69 (1), 24-32 (2003)
   PUBMED   12513973
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Reid,S.J., Abratt,V.R. and Trindade,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2001) Molecular and Cell Biology, University of
            Cape Town, Private Bag Rondebosch, Cape Town 7701, South Africa
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Bifidobacterium animalis subsp. lactis"
                     /mol_type="genomic DNA"
                     /sub_species="lactis"
                     /db_xref="taxon:302911"
                     /note="synonym: Bifidobacterium lactis"
     gene            complement(771..2372)
                     /gene="scrT"
     CDS             complement(771..2072)
                     /gene="scrT"
                     /note="sucrose symporter"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|J9W1F3|2.A.1.2.93"
                     /protein_id="AAN01604.1"
                     /translation="MVSADGHRPTKAELVRLGIGFTVSAVACAIPWVALSTVILPRVFE
                     LIDNSSKEAMLGLVNSIGAIVALLANIIFGTLSDMTRSRFGKRTPWIVGGGIITGLAIG
                     AIAFTRNEALIIFLWCVAQMGYNMMLAPYVAVMSDRVPDKVRGTISGFYGAGIAVGQTL
                     GSVVGAAFLRQGQSGLFSAWMMGLGIFSLTGIFVVAMWPREKSSRFEEQSEHVTVKSVL
                     LNFRPPRHAPDFYYALVGRTLMMGGYWMITTYQLYIAQDYIYAGDPNAKDKAATIIATM
                     GVITLVVSLIAAVTAGPITDRLGRRKLPVALASCLFAVGAAMPLFFRSPMGMYLFAAVA
                     GLGYGVYNAIDQALNVAVLPNPKEAGKDLGILNLANTLSTVIGTAMTSLVVSIVKSSMH
                     VSTTPAVAYAWVFGVAIVIVLAAAALIMRIRNVK"
     gene            complement(2441..3961)
                     /gene="scrP"
     CDS             complement(2441..3961)
                     /gene="scrP"
                     /note="sucrose phosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13|GH13_18"
                     /protein_id="AAN01605.1"
                     /translation="MKNKVQLITYADRLGDGNLASMTDILRTRFDGVYEGVHILPFFTP
                     FDGADAGFDPIDHTKVDARLGDWDDIAELAKTHDIMVDAIVNHMSWQSKQFQDVLANGE
                     DSEYYPMFLTMSSVFPDGATEEELAGIYRPRPGLPFTHYSFAGKTRLVWVTFTPQQVDI
                     DTDSAKGWEYLMSIFDQMSKSHVKYIRLDAVGYGAKEAGTSCFMTPKTFELISRLREEG
                     AKRGLEILIEVHSYYKKQVEIAAKVDRVYDFALPPLLLHSLFTGKVDALAHWTEIRPNN
                     AVTVLDTHDGIGVIDIGSDQLDRSLKGLVPDADVDNMVETIAKNTHGESKAATGAAASN
                     LDLYQVNSTYYSALGCNDQHYIAARAVQFFLPGVPQVYYVGALAGENDMELLKRTNVGR
                     DINRHYYTTSEIDKNLERPVVKALNALARFRNELPAFDGDFSYSVGDDESIAFSWNGFG
                     SFPTLTFTPSKGMGVENPQSVATLVWTDSTGEHRTDDLIANPPVMQAS"
     gene            4267..5247
                     /gene="scrR"
     CDS             4267..5247
                     /gene="scrR"
                     /note="sucrose regulator; LacI-GalR family regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="AAN01606.1"
                     /translation="MVGMRDVAKRAGVSVSSVSLVINGTGYVSRDMREKVEQAMRELDY
                     VPNELARNFYHGKTDIIGVIVPTIQHPFFATLTAHLQHEFADRGLQTMLCSTADSANGE
                     AQYVDMLRRHSLDALVVAAHTTHDPQYWRAIDRPVVAFDRNLGAGITQVSSDHVHGGEL
                     IAELLVRTGAHHVVMVGGPRAQFTDLGDHSTFPTVRYVQTLEDRLSQARIAHTYIESGE
                     VFDIAGVRRAVREAFDTYPDMDAFVGADLAAAFAVQEAVSRSIAVPRDVQIIAYDGTLA
                     ADCAGMPLTTVAQDFRQIAHTIAACVEHGIESSPQRPDATDAMPT"
ORIGIN
        1 gcccgagcac cacgccctgt accgtgtccg gcggaatcgt gcggaagaag tagtcgttga
       61 gacccgagaa cgcggtggat gtggcgcccg acgagatcat cggcacctgc gacgacgtca
      121 cctgcttgaa cgtgttcttc accaccgagc tcgacggcgg cccgaccacg accgacggct
      181 ccctggcgag caccgactgc gccgacgacg tgttctggtc cgcgtggtcg gcgtccgagg
      241 tgtcggccga gacgatatcg atgtccttgc cgagcacgcc gccggccgcg ttgatgtcgt
      301 tctgtgcgag cttcatcgtc gcgatgttcg ccggtcccaa atatgccatg gagccggtga
      361 gcgggaggag cccggccgcc gtgaacgtgg cctcgtcggc gtccgccttg ctttccgcgc
      421 ccgagccgca gcccgacagc gtgagcatcg ccgccgcggc cattgccgtc gccgtgacca
      481 tccatgactt gattttcgca ttgttcattg caatcaccgt ttcctctctc tgatctcttg
      541 cccgcgcgaa tctgcgaatg caactcttcg caagcaggcg aacggtggtt tcgattcaca
      601 tccctagact tcttgcgtga atctgtctgc agattacgtg cgccacaaac cgagcgcgtg
      661 agccgcgtta cgctcgcatg aacgcggaaa agcgttcgaa acacaaggtg atatggaaca
      721 ggccggcaac gcgaacgctg ccggcctgcg gtctggagag aggtaaggga ttacttgacg
      781 ttgcggatgc gcatgatgag tgcggccgcg gcgagcacaa tcacgatcgc gacgccgaac
      841 acccatgcgt aggcgacggc gggcgtggtg gagacgtgca tcgagctctt gacgatggag
      901 acgacgagcg aggtcattgc cgtgccgatc actgtggaaa gcgtgttggc gaggttgaga
      961 atacccagat ccttgccggc ttccttcgga ttcggcagca cggccacgtt gagcgcctgg
     1021 tcgatcgcgt tgtagacgcc gtaaccgagg ccggcgacgg cggcgaacag atacatgccc
     1081 atcggcgagc ggaagaacag cggcatcgcg gcaccgacgg cgaacaggca gcttgcgagc
     1141 gccaccggca gcttgcgcct gccgagacgg tcggtgatcg ggcctgccgt gacggcggcg
     1201 atcagcgaga cgacgagcgt gatcacgccc atggtggcga tgatcgtagc cgccttgtcc
     1261 ttcgcattcg gatcgccggc gtagatgtag tcctgcgcaa tgtacagctg gtaggtcgtg
     1321 atcatccagt agccgcccat catgagcgtg cggccgacga gcgcgtagta gaagtcgggg
     1381 gcgtggcgcg gcggccggaa gttcaggagc acggacttca cggtcacatg ctcgctctgc
     1441 tcctcgaagc ggctcgactt ctcacgcggc cacatggcga cgacgaagat gccggtgaga
     1501 ctgaagatgc ccagacccat catccatgcg gagaagagtc ccgactgccc ctgacgcagg
     1561 aacgcggcac ccacgaccga gccgagcgtc tggccgacgg cgatgccggc gccgtagaag
     1621 ccggagatcg taccgcgcac cttgtcgggc acacggtcgg acatcacggc cacgtatggc
     1681 gcgagcatca tgttgtagcc catctgggcg acgcaccaca ggaagatgat caacgcctcg
     1741 ttgcgcgtga atgcgatggc cccgatggca agaccggtga tgatgccgcc gcccacgatc
     1801 cacggcgtgc gcttgccgaa gcgggagcgc gtcatgtcag acagcgtgcc gaagatgatg
     1861 ttcgccagca atgccacaat cgcgccgatc gagttgacca agccgagcat ggcttccttg
     1921 ctcgagttgt caatcagctc gaacacacgc ggcagaatga cggtgctcag cgccacccac
     1981 ggaatcgcgc aggcgactgc ggaaacggtg aagccgatgc ccaagcgcac gagttcggcc
     2041 ttggtcgggc ggtggccgtc ggccgagacc atcggatcgc gcatgtccat gaatgcggac
     2101 gcctcgtccg gcaccgccgt gtccaccgcg gccagcgccg ccatgccgga gccgacggcc
     2161 tgcgaatcgg tggcgagttt ggtgccctgc acctgctcgt cgcccacact gctgtcggcg
     2221 gctcgcttct tcggcatcgg cgcggtgaga cgggagatgt tctccttgtc ttcctggctc
     2281 aataccttgt cgaagtcggg gcggtcgtcg atgcccgggc gaatgtagcc gttcgagccc
     2341 gtcgacggcg cgctccctcc gtgggtgtct tgttcgtcaa ccatgatttc tcttttccgg
     2401 gaatctgaaa atatctggaa tctctatcgg gaaccgcgca tcagctcgcc tgcatgacgg
     2461 gcgggttcgc aatgaggtcg tcggtgcggt gctcgccagt ggaatccgtc cagacgagcg
     2521 tggcgacgga ctgtgggttc tcgacaccca tgcccttgga cggcgtgaac gtgagcgtag
     2581 ggaaggatcc gaaaccgttc caggagaacg caatcgactc gtcatcgccc accgagtagc
     2641 tgaagtcgcc gtcgaacgcc ggcagctcgt tgcggaatct ggcgagcgcg ttcagggcct
     2701 tgaccaccgg acgttcgagg ttcttgtcga tctcggaggt cgtgtaatag tggcggttga
     2761 tgtcgcggcc cacattcgtg cgcttgagca gctccatgtc gttctcgccg gcgagcgcgc
     2821 ccacatagta cacctgcgga acgcccggca ggaagaactg cacggcgcgt gcggcaatgt
     2881 aatgctggtc attgcagccg agcgccgaat aatatgtgga gttcacctgg tagaggtcga
     2941 ggttcgaagc ggcggcgccg gttgcggcct tcgactcgcc atgggtgttc ttcgcgatcg
     3001 tctcgaccat gttgtcgacg tccgcgtcgg gcaccagacc cttgagcgaa cggtcgagct
     3061 ggtcggagcc gatgtcgatg acgccgatgc cgtcgtgcgt gtcgagcacg gtgaccgcat
     3121 tgttcgggcg aatctcggtc cagtgcgcga gcgcgtccac cttgccggtg aacagcgagt
     3181 gcagcagcaa tggcggcagc gcgaagtcat agacgcgatc caccttggcg gcgatctcca
     3241 cctgcttctt gtagtacgag tgcacctcga tgagaatctc cagaccgcgc ttggcaccct
     3301 cctcgcgcag tcgcgagatg agttcgaagg tcttgggggt catgaagcag ctggtgccgg
     3361 cctccttggc gccgtagccg acggcgtcga ggcgaatgta cttgacgtgc gacttgctca
     3421 tctggtcgaa gatcgacatc aggtattccc agcccttggc ggagtcggtg tcaatgtcga
     3481 cctgctgcgg cgtgaacgtg acccacacca agcgggtctt gccggcgaag ctgtagtggg
     3541 tgaacggcag gcccgggcgc gggcggtaga tgccggcgag ctcctcctcg gtcgcgccgt
     3601 cggggaacac ggagctcatc gtgaggaaca tcgggtagta ctcggaatcc tcgccgttcg
     3661 cgagcacatc ctggaactgc ttggactgcc agctcatgtg gttgacgatg gcatcgacca
     3721 tgatgtcatg cgtcttggcg agctcggcga tgtcatccca gtcaccgagg cgggcgtcga
     3781 ccttggtgtg gtcgatcggg tcgaagcctg cgtccgcgcc atcgaacggg gtgaagaacg
     3841 gcagaatgtg cacgccctcg tacacgccgt cgaatcgcgt gcgcagaatg tcggtcatcg
     3901 aagcaaggtt gccgtcaccc aagcgatccg cataggtgat cagctgaact ttgttcttca
     3961 tggctcctcc tggattcgtc tatcacgtct tcgggatatc tccggggcga tgcgtctgcc
     4021 tgaagcttca tcgttgcgcg agtgccgtat cgaaccggtt cgagtattgt tttacctcat
     4081 tcttcacgcg catgcaagcg ctctgctctc ggcgtatcaa accggttcga tattcgacga
     4141 ttcaccgcgc tctgccgaaa cgcggttgcc ggaggacgaa atctgcacat acaatcggat
     4201 agtgctcgtc aaaccggttc gataccgcgg gcgatagcgc acggagcaga aaatgaggag
     4261 gcgaacatgg tcggcatgcg cgatgtcgcg aaacgggcgg gcgtctcggt gagcagtgtc
     4321 tcgctggtga tcaacggcac cggctatgtc tcacgcgata tgcgcgagaa ggtcgagcag
     4381 gccatgcgcg agctcgacta tgtgcccaac gagctcgcgc gcaatttcta ccacggcaag
     4441 actgacatca tcggcgtcat cgtgcccacc atccagcacc cgttcttcgc cacgctcacc
     4501 gcgcacctgc agcacgagtt cgcggaccgc ggtctgcaga cgatgctgtg ctccaccgcc
     4561 gactccgcga acggcgaggc gcagtacgtc gacatgctgc gtcggcacag tctggacgcg
     4621 ctggtcgtcg ccgcgcacac cacccacgac ccgcagtatt ggcgcgcgat cgaccgcccg
     4681 gtggtcgcct tcgaccgcaa tctgggggcg gggatcactc aggtgagttc agaccacgtg
     4741 cacggcggcg agctcatcgc agaactcctc gtgcgcaccg gcgcgcacca tgtggtcatg
     4801 gtgggcggcc cgcgcgcgca attcaccgac ctcggcgacc actccacctt ccccaccgtc
     4861 cgctatgtac agacgttgga agaccgcctt tcccaagcgc ggattgcgca cacgtacatc
     4921 gagtcgggcg aggtgttcga catcgccggc gtgcggcgtg cggttcgcga ggcattcgac
     4981 acatacccgg acatggatgc gttcgtgggg gcagatctcg ccgccgcatt cgccgtgcag
     5041 gaggcggtct cgcgctcgat cgcggtgccc cgtgatgtgc agatcatcgc ctacgacggc
     5101 acactcgccg cggattgcgc cggcatgccg ctcaccacgg tcgcccagga tttcaggcag
     5161 atcgcgcaca ccatcgccgc gtgcgtggaa cacgggatcg agtcttcgcc gcaacgcccg
     5221 gatgccacgg acgcgatgcc aacatgacga tcatcccggt gacgctgcac gagcgcgcca
     5281 ccacccgtcg ctgaggtcgg cgcccgaccc gcaaaaacag cgaagggccg ccttgtgggc
     5341 agcccttcgt atagaggaga gggatggtaa gtcactcggt cggcacgtcg ccgctctgca
     5401 tgtcctggaa gacgttgacg ttgttgccgt cgtactcgaa gatgccgatg ttcgccgagc
     5461 tcgggtcgtt gttcttgttg aagggcccga tgccggtctt gccgaagtac tggatcttct
     5521 tcttgtcttt gatgagcgcc ttgcaggcct cgaagctctc gcacttctcc gccgtcggcg
     5581 ccggagaccg aggagaggtt cttcgcgacc gtctcgccgc tggtgctcgc cctgttcggc
     5641 ggcgagcgcg gcgaggatca ctgcgtcgta ggtttcggcg gcgtatgtgg tgtcggagat
     5701 gtcggtgccg gtgcccttga ggtcggactt gaacttgtcg tcgacatggg tgccggggat
     5761 ggtgcccttc gagcccttga gcaggccggc gtcgtaggtc ttcgagtagt cgacggtgtt
     5821 gccgtcgact agatagagct tgtgcgtgtc cacgccggcg gagccagcgc cttgatcaag
     5881 cgcgtcgcct gatctcataa
//
