LOCUS       AF132735               14184 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Clostridium cellulovorans endoglucanase K (engK), hydrophobic
            protein A (hbpA), endoglucanase L (engL), mannanase A (manA),
            endoglucanase M (engM), endoglucanase N (engN), and transposase
            (trp) genes, complete cds; and malate permease (mln) gene, partial
            cds.
ACCESSION   AF132735
VERSION     AF132735.2
KEYWORDS    .
SOURCE      Clostridium cellulovorans 743B
  ORGANISM  Clostridium cellulovorans 743B
            Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 14184)
  AUTHORS   Tamaru,Y. and Doi,R.H.
  TITLE     The engL gene cluster of Clostridium cellulovorans contains a gene
            for cellulosomal manA
  JOURNAL   J. Bacteriol. 182 (1), 244-247 (2000)
   PUBMED   10613891
REFERENCE   2  (bases 1 to 14184)
  AUTHORS   Tamaru,Y., Karita,S., Ibrahim,A., Chan,H. and Doi,R.H.
  TITLE     A large gene cluster for the Clostridium cellulovorans cellulosome
  JOURNAL   J. Bacteriol. 182 (20), 5906-5910 (2000)
   PUBMED   11004194
REFERENCE   3  (bases 1 to 14184)
  AUTHORS   Tamaru,Y., Ibrahim,A., Karita,S. and Doi,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-1999) MCB, University of California, Davis, CA
            95616, USA
REFERENCE   4  (bases 1 to 14184)
  AUTHORS   Tamaru,Y., Ibrahim,A., Karita,S. and Doi,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2000) MCB, University of California, Davis, CA
            95616, USA
  REMARK    Sequence update by submitter
COMMENT     On Mar 31, 2000 this sequence version replaced AF132735.1.
FEATURES             Location/Qualifiers
     source          1..14184
                     /organism="Clostridium cellulovorans 743B"
                     /mol_type="genomic DNA"
                     /strain="ATCC 35296"
                     /type_material="type strain of Clostridium cellulovorans"
                     /db_xref="ATCC:35296"
                     /db_xref="taxon:573061"
     gene            92..2770
                     /gene="engK"
     CDS             92..2770
                     /gene="engK"
                     /EC_number="3.2.1.4"
                     /note="cellulase; family 9 glycosyl hydrolase; family IV
                     CBD; contains a dockerin domain"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM30|CBM4|GH9"
                     /protein_id="AAF06107.1"
                     /translation="MRSKKLIACVTALATVLSVSTVATSVATTKTVSAATMVSVGELIR
                     NNKFDNGVGLPWTVVESYPAKSSFEIKDGKYYVTVLQDGVEGRWDVQFRHRGLVIEQGH
                     KYRVKFTVTADKDCYVYPKIGDQGEPYKEYWNYNSYQRVQLRAGVPTTIDQTFEMRDAT
                     ARTAEFAIHLAGDCKAATFPYTFTFDDMYVSDPQFPGYVAETPEPTNAIRVNQVGYLPG
                     VAKKATVVTKATTPINWYLKNSSGVQVATGTTTVKGLDSASGDNVHIIDFSNYTTPGTG
                     YTLSVDSTNVDSTINDSASSMPFTIGTDLYSKMKHESIKYFYLNRSAIPITMPYAERTD
                     LTRAAGHTTDLMPTDASSSDPSPWYKENYSLDVTGGWYDAGDHGKYVVNGGISVWTMMN
                     QYERAKKLGQANVAPFADNTMNIPESGNGYPDILDESRFQMDLMMKMQIPAGKTYAGMA
                     HHKGHDERWTALAIRPDQDPMKRYLKAPSTAATLNLAATAAQASRLWKGIGDAYSAKCL
                     ASAETAWKAAKASPAIYAPFENGPGGGAYGDDNVTDEFYWAAAELYETTGTSEYLDYMK
                     NNSSDKFLKMPTTLTGGEDKGLSGAFDWGNVAGLGTISLAIGDKLDATSKATVRANVAA
                     AADVFVANTNSEGYGTPMVQGPAFEEKDASGKVIRTITGYPWGSNSFVANQAIVMGYAY
                     DFTKGDADKKKSNSYFNGLTSAMDYLLGRNPMVQSYVTGYGSNPLENPHHRFWAYQADN
                     TFPKAPAGCLSGGPNTGLQDPWVKGSGWGVGTKPAAKCFMDNIESWSTNEITINWNAPI
                     AWMSSYMDLNKDAKTIDITIPPTTSGDVNGDTKINAIDLAMLKKYILDNSTVIKTANAD
                     MNNDGKINAIDLALLKKKLLS"
     gene            2918..3640
                     /gene="hbpA"
     CDS             2918..3640
                     /gene="hbpA"
                     /note="cohesin"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobic protein A"
                     /protein_id="AAF06108.1"
                     /translation="MKKNILIITLVVSIGVVVAGVAMINSNSKDSSSAVVEPVDSNKTT
                     TDDTNSTDTNNTSSKASENTDNSTNNTDNTENSTPATNNNPSGETVSNPGQSSEVKASN
                     ESVSVAIGEVNGSVGSEIIVPVTITDTPKSGIGSCDFKVNYDTTALELIEITPGDVLVN
                     PEANFSSSSDQNLGIASFLYLDNTFEKEAITKNGVFANIKFKVKDGATGAKEVAFKSIG
                     AFTDNALSSHEVKTTNGK"
     gene            3874..5442
                     /gene="engL"
     CDS             3874..5442
                     /gene="engL"
                     /EC_number="3.2.1.4"
                     /note="cellulase; family 9 glycosyl hydrolase; contains a
                     dockerin domain"
                     /codon_start=1
                     /transl_table=11
                     /product="GH9"
                     /protein_id="AAF06109.1"
                     /translation="MKKIIATATSLCLSAGIILPATEAAAAPKFDYSDAFGKSIMFYEA
                     NWSGKVENNRFDWRGDAFLKDGADVGLDLSGGFFDAGDHVKFGLPQAYAASTLGWGYFA
                     FKDAYKSNGQDAYMLKILKHFTDYFLKCYPDNNTFYYQVGDGDLDHAYWGPPEIQPTAR
                     PTKFKATPSDPASDVCGNTAAALAIMYLNYKDVDATYANKCLTAAKNLYSLGKNYKGLS
                     HGQSYYVSTSYYDDLAWGAVWLYQATNDNSYMTDANSFLSQINADGDKVYTSYWTHCWD
                     NVWTGVIYKMAEVTGKANYKASVENNLNYWMNNIKTTAGGLKFATDWGSLRYSSTQAML
                     ALIQYNRTKEQKYLDFATRQIDYILGDNPRSSSYVVGFGNNYPKFPHHRGASGRLEPGE
                     MKTMPEKHILTGALVGGPDGNDQYKDDINNYQQTEVAIDYSAGFVGAMAGITKYLDPAP
                     IIIDPITYALGDVNKDGKVNAIDLAILKKNLLNGITGDLTVSDMNKDGKVNAIDLALLK
                     KLLLG"
     gene            6013..7290
                     /gene="manA"
     CDS             6013..7290
                     /gene="manA"
                     /EC_number="3.2.1.78"
                     /note="family 5 glycosyl hydrolase; contains a dockerin
                     domain"
                     /codon_start=1
                     /transl_table=11
                     /product="GH5_17"
                     /protein_id="AAF06110.2"
                     /translation="MKRRILSILTAALVTATIIPFSASAATTLGDVNVDGKVNSIDMAI
                     VKQYILGTKTFSDAELKAADVDANGKVNAIDLALYKQYILGSITKFPGDKGVVDNRTTT
                     GPGITWMDGYKLFPVGVNYGWNNWCYEFSDSNWDTNFAKMKSDMDAMNLKGVKSLRWWV
                     FPDLAFGPTWSGTGKGSLCTGLPNNWVNHMKAVCDYALTKNIKIYWTLTSFDAARQDGT
                     DHHSDIFTNEAVRQSFFDNAVKPIVTALGNHEAVMGWDCINEPEWIIKKEDNGEPKASG
                     YDIYALSDIRNYVQSTTALIHQYAKQPVSVGSASMKWSGAQFSLWTGLGLDFYDFHWYD
                     WATPWFNPMTTPASSLKLDKPVIIGEMMSDTLSSSLKVDHKVVMDAIMANGYSGYMTWA
                     WNDGSCGNFIGSNFTNFGAQHTDIVR"
     gene            8125..10755
                     /gene="engM"
     CDS             8125..10755
                     /gene="engM"
                     /note="cellulase; family 9 glycosyl hydrolase; family IV
                     CBD; contains a dockerin domain"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM4|GH9"
                     /protein_id="AAF06111.2"
                     /translation="MNRKKITAYATAIVTVLSIPTVATSVVTSKSVSAATNFDYREIIA
                     NNTFDNNNATPWTLVQNYPAIAKMVAKDGKCSVTVLNDGVEGRWDVQLRHRGIVLDQGH
                     KYRVRFTVTADKDCYIYPKIGDQGEPYREYWNYNSYQRIQLKAGVPTTVDQTFEMKDPT
                     AKTAEFAFHLAGDCKAATFPYTFTFDDIYLTDPNRIPPPPPPEEPTNAVRVNQVGYFTN
                     LQKKATVVTKATSPITWYLKNSAGVQVATGETKVLGADAASGDNVHIIDFSNYTTEGSD
                     YTLSVDQSNIDSLICNSASSLPFAIGKELYNKLQKDAMKYFYLNRSGIPITMPYAERTD
                     LTRVAGHTLDVIATDASSTDPSPWYKENYSLEVTGGWYDDTRYNKSVINGSISAWTIMN
                     QYERALKANKVSVIPFADNTMNIPESGNGYPDILDEVRFQMEMMMKMQIPAGKTYSGMV
                     HHRAKDERWIPLATRPDQDQMKRYLEPPSTAATLDLAATAAQSSRLWAKYDSTFANQCL
                     VSAETAWAAAIKNPAIYAPLQSSFSGTYGDTNIADEFYWAAAELYETTGKQQYLDYLKN
                     NCQDKFLKIPTKIVGGEANGVSGCFDSGDVTGLGTISLAIGDKLDVASKTTARANIAAA
                     ADVFVANTQKEGYGTPMDQGIVGQVVDQNGYVTKPIEGYPYASNSYVTNEAIVMGYAYD
                     FTKNRNYLNGVVSAMDYILGRNPMVQSYVTGYGSNPVENPHNVFWAYQYDNTFPKAPAG
                     CMASGPNSGLQDSWVKASGWTPGSRPAAKCFMDNIESWSTNDLSNSYNASLAWVSSYVN
                     LNSLPESIKGDINNDAKINAIDLALLKKYILNNDIPIVAINGDINGDGIINAIDLLLLK
                     KQLLA"
     gene            11112..11717
                     /gene="engN"
     CDS             11112..11717
                     /gene="engN"
                     /note="family IV CBD"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM4"
                     /protein_id="AAF61310.1"
                     /translation="MNKKKIMAYATAFVTLLSVPTVVTSVVATESVSAFTRINLGELIR
                     NNTFDYNVATPWQLTKISPAEASIEVKDGKCYVTILKNGEDGRWDIQLRHRGIVLDQGH
                     KYRVRFTVTADKDCYIYPKIADQGGSFNEYWNYNTYQKIQLNAGVPTTIDQIFQMNNAT
                     TRSAEFVFDLAGDCQAATFPYKLTFDDIYLTDLNHIQY"
     gene            complement(12101..13042)
                     /gene="trp"
     CDS             complement(12101..13042)
                     /gene="trp"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AAF61311.1"
                     /translation="MPEFNKDSTITINDLKDFIVVTYVIIDDFYQKVTPTFIKNRRNIA
                     KSVMTDSEIITISLVGELLTIDSEKAWFGFCSKNLRDLFPNFCSRPRFHRVRKSLFRVI
                     DEIRKELTKFLNYQYDRMRIADSMPIPVCKFGRAHFHKAFKPEAAYGRCASKKETYYGF
                     KLHALVALDGYITDFTVTAANIDDRDVVWELTANSEIDILIGDKGYIGQKVASQLKETR
                     YIRLLTINRNNSKTKLLKPFRQLIFKARRRVETTFSQLSEQLNMQRVLTKSTWGFATRI
                     SNKILAHNLCYFINKFFNIGIEISKIKELVFG"
     gene            13314..>14184
                     /gene="mln"
     CDS             13314..>14184
                     /gene="mln"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q48797|2.A.69.4.5"
                     /protein_id="AAF61312.1"
                     /translation="MVIIQSIQSIMTILLMISVGYILDRKKWFNEESKKLLSKLVVNIS
                     LPAYMLQNIVTNFTREKLFEMSGGLLIPFVSIGICYIVAVVIAKAIKVPKKRFGTFVTL
                     FFVSNTIFIGLPVNLALFGEQSIPYVFLYYIANTTFFWTLGVYSISKDGDAEDTKLFSI
                     ASLKKCFSPPMLGFIIGIIFVIINLKIPFFILDTCKYLGNLTTPLSMLFIGATMAAVDL
                     KTIRIDKEMIFLNIGRSVISPLIVIILGLFIKGPMLMKEVFIIQAAMPGMINISIISKA
                     YNADYEYAA"
ORIGIN
        1 aaagttcctt taaaatccaa aatccttgat ataaataaaa tcaatcaatc aattaatcaa
       61 tcaatgaatt taattttagg agggtctatg aatgcgtagt aaaaaattaa tagcttgcgt
      121 aacagcatta gccacagtac taagtgtttc aacagttgct acttcagttg ctactactaa
      181 aactgttagt gcagcaacaa tggtaagtgt tggagaatta atcagaaaca ataaatttga
      241 caatggtgtt gggttaccat ggacagttgt tgagtcttac ccagcaaaat ctagctttga
      301 aatcaaagat ggaaaatact atgtaacagt tcttcaagac ggtgtagaag gaagatggga
      361 tgttcaattc cgtcacagag gtcttgttat agaacaagga cataaataca gagttaaatt
      421 tactgtaact gctgataaag attgttacgt ttatccaaaa ataggtgacc aaggtgaacc
      481 ttataaagaa tattggaact ataactcata tcaaagagta caattaagag ctggtgttcc
      541 aacaacaatt gatcaaactt ttgaaatgag agatgcaaca gctagaacag ctgaatttgc
      601 aattcacctt gctggtgact gtaaggcagc aacattccct tatacattta cttttgatga
      661 tatgtatgta tcagatccac aattcccagg atatgtagct gaaactccag aaccaacaaa
      721 cgcaataaga gttaaccaag taggttactt accaggcgta gcaaagaaag ctacagttgt
      781 tacaaaagca acaactccaa taaactggta cttaaagaat agttcaggtg ttcaagttgc
      841 tactggcaca acaacagtta aaggcttaga tagcgcttca ggagataatg tacatattat
      901 agatttctca aattacacaa ctccaggaac tggctacaca ttatcagttg attcaacaaa
      961 cgttgattca acaattaatg attctgcttc aagtatgcct tttactatag gaacagattt
     1021 atattctaaa atgaaacatg aatcaataaa atacttctac ttaaacagaa gtgctattcc
     1081 aataacaatg ccatatgctg aacgtactga tttaactcgt gcagcaggac atacaacgga
     1141 tttgatgcca actgatgcaa gttcaagtga tccaagtcct tggtataaag aaaattatag
     1201 tctagatgtt acaggtggat ggtatgacgc tggagaccac ggtaagtacg ttgttaacgg
     1261 tggtatctct gtttggacaa tgatgaacca atatgaaaga gctaagaaat taggacaagc
     1321 taatgtagct ccatttgcag ataatacaat gaatatccca gaaagcggaa acggctatcc
     1381 agatattctt gatgaatcac gtttccaaat ggatttaatg atgaagatgc aaattccagc
     1441 tggtaaaact tacgctggta tggctcacca taaaggtcat gatgaaagat ggacagctct
     1501 tgcaatccgt ccagaccaag atccaatgaa acgttactta aaagcaccaa gtacagctgc
     1561 aacattaaac cttgctgcta cagctgcaca agcttcacgt ttatggaaag gtattggtga
     1621 tgcatattct gctaagtgtt tagcgtctgc tgaaactgca tggaaagcag ctaaggctag
     1681 tccagcaata tatgcaccat ttgaaaatgg tcctggtggt ggagcttacg gtgatgacaa
     1741 cgtaacagat gaattctact gggcagcagc agaactttat gaaacaactg gaacatcaga
     1801 atatcttgac tacatgaaga ataattcttc agataaattc ttaaagatgc caactacatt
     1861 aactggtgga gaagacaaag gattatcagg tgctttcgac tggggtaatg ttgctggttt
     1921 aggaacaatt tctcttgcta ttggtgataa attagatgct acatcaaaag caactgtaag
     1981 agctaatgtt gctgccgctg ctgatgtatt tgtagctaat acaaatagtg aaggttatgg
     2041 tactccaatg gttcaaggac cagcatttga ggaaaaggat gcaagtggaa aagttataag
     2101 aacaataact ggctatccat ggggttcaaa ctcattcgta gctaaccaag ctatcgttat
     2161 gggttacgct tatgacttta caaaaggcga tgcagataag aaaaaatcta attcgtactt
     2221 taatgggtta acaagtgcta tggactattt attaggacgt aacccaatgg tacaatcata
     2281 cgttactgga tatggttcta acccactaga aaatccacat caccgtttct gggcatacca
     2341 agctgacaac acattcccta aagctccagc aggatgttta tcaggtggtc ctaacacagg
     2401 attacaagat ccatgggtta aaggctcagg ttggggagtt ggtactaagc cagctgctaa
     2461 atgcttcatg gacaatatag aatcatggtc aactaatgaa atcacaatca actggaatgc
     2521 accaattgca tggatgtctt catacatgga tttaaataag gatgcaaaaa caatagatat
     2581 tactattcca cctactacat caggtgatgt aaacggagat actaagataa atgctatcga
     2641 tttagctatg ttaaagaaat atatcctaga caattcaact gtaatcaaaa cagctaacgc
     2701 tgatatgaat aatgatggaa aaattaatgc tatagattta gctttattaa agaagaaact
     2761 tctttcttaa taaaaatagc taagaattaa gtttttccta agagattact agataaataa
     2821 taaaataaaa actgtagaaa gttcaccttt ttacagtttt tattctaaag atttttatta
     2881 aaagatgtaa ggactaatta ataaaggaga tacagacatg aaaaaaaaca ttcttataat
     2941 tacattagta gtgagtattg gcgtagtagt tgcaggcgtt gcaatgatta attcaaacag
     3001 taaagattct agtagtgcag ttgtagaacc agtagattct aataaaacta cgactgatga
     3061 cactaatagt actgatacta ataacacttc ttcaaaggct agtgaaaata ctgataatag
     3121 tacaaataat actgataata ctgaaaatag tactccagct accaataata atccatctgg
     3181 ggagactgtt agtaatccag gacaatcaag tgaagttaaa gcatctaatg aatcagtttc
     3241 agtagcaatt ggtgaagtaa atggttctgt aggaagtgaa attattgttc cggttactat
     3301 tacagataca ccaaaatcag gaataggaag ttgtgatttt aaggttaatt atgataccac
     3361 agcacttgaa ttaattgaga ttactcctgg agatgtatta gtaaatcctg aagcaaattt
     3421 tagtagtagt tcagatcaaa atttaggaat agctagtttt ttatatttag ataatacttt
     3481 tgaaaaagaa gcaataacta aaaatggtgt ctttgctaat ataaaattta aggttaagga
     3541 tggagctaca ggtgctaaag aagtagcgtt taaatctatt ggagctttca ccgataacgc
     3601 acttagcagc catgaagtaa aaactacaaa tggaaagtaa atattaatta gtatctttac
     3661 tgagacattc atcactttag aaaaaatata aataataggc tctttgtata aaggagccca
     3721 ataattatct tatcaatcta gatgaaaata gaaatgttta agaatgtaaa cggtatagga
     3781 gaaatttatc aggatataac ggggagaaaa aataattctg agatttaaac tattctgaga
     3841 aaaattagaa atatataaat agggggattt tttatgaaaa agattatagc aacggcaacg
     3901 tcgttgtgtt tatcagcagg aataatatta ccagcaactg aagctgccgc agcacctaaa
     3961 tttgactatt ctgatgcttt tgggaaatca attatgttct atgaagctaa ttggagcgga
     4021 aaggtagaaa ataatagatt tgattggaga ggagatgcct ttttaaagga tggtgccgat
     4081 gttggacttg acctttcagg aggttttttt gacgcaggag atcatgtaaa gtttggatta
     4141 ccacaagctt atgccgcttc aactcttggt tgggggtatt ttgcgtttaa ggatgcttat
     4201 aagtcaaatg gacaagatgc atatatgctt aaaattttaa aacattttac agattatttc
     4261 ttaaagtgtt acccagataa taatacattc tattatcaag tgggagatgg agatttagac
     4321 catgcttact ggggaccacc agaaattcaa ccaacagcaa gacctacaaa atttaaagca
     4381 actccatcag atccagcttc agatgtatgt ggtaatactg ctgcagcact tgcaataatg
     4441 tatttaaatt ataaggatgt agatgctacc tatgcaaaca aatgtttaac agctgcaaag
     4501 aatctatatt cattaggtaa aaattataaa ggattaagtc acggccaaag ctattatgta
     4561 tctacaagct attatgatga tttggcttgg ggtgctgttt ggctatatca agcaacaaac
     4621 gataatagct atatgactga tgcaaattca ttccttagtc aaataaatgc tgatggtgac
     4681 aaagtttata catcatattg gacacattgt tgggataatg tatggactgg agtaatttat
     4741 aagatggcag aggttacagg aaaagcaaac tataaagctt cagttgaaaa caatttaaat
     4801 tattggatga ataatataaa aactactgct ggtggattaa agtttgctac agattggggt
     4861 tcgttaagat actcatcaac tcaagccatg ttagctttaa tacagtacaa cagaactaag
     4921 gaacaaaaat atttagactt tgcaacacgt caaatagatt acatattagg agataatcca
     4981 agaagcagtt cttatgtagt tggatttgga aataactatc ctaagttccc acatcataga
     5041 ggagctagcg gaagacttga accaggtgaa atgaaaacta tgccagaaaa gcatattctt
     5101 actggagcgt tagttggtgg acctgacgga aatgatcaat acaaagatga tataaataat
     5161 tatcaacaaa cagaagttgc tatagattat agtgctggtt ttgtaggtgc aatggcaggt
     5221 attacaaagt atttggatcc tgcaccaata ataatagatc caataacata tgcgctaggt
     5281 gatgtaaata aagatggtaa agtaaatgcc atagacttag ctattcttaa aaagaacctt
     5341 ttaaatggta ttactggaga cttaactgtt tctgatatga ataaggatgg caaggtaaat
     5401 gcaatagatt tagcacttct taaaaagtta cttcttggtt aaatttatta ttaaaatttt
     5461 attaagaaga taaaaataag gtgtatataa agagagactc caatgtctaa tttctataac
     5521 taacaatagt tttccaattg ctttagaatt aactaaagca attggagact tagaatagag
     5581 ttctaaaaag cttatcaata gcagagaaac taccatcgta ttaatagcga tattgaacta
     5641 gaaattgaga aaagcttagg aatagttcat atgttgtagg ctttagaaat aactatccga
     5701 gggttcaata ttataagaat tatagaggaa tgcttttaaa ctgtaagttt attaaaataa
     5761 gaaatacttt taaagaattt ttataaagat ttagtttgga cactatagtc tgcggatagt
     5821 tattctgatg aggaagttag ctatttatga ggagagactt ttattattac gtgtttatat
     5881 attgaatagt aaacttattt tgtaaaataa ggaattgata tttattaagt ggatacgaat
     5941 aaggatggag agtttaacac ttttaattta gtgttaaaat aaataattta tgaaaatgtg
     6001 gagggtaaag aaatgaaaag gagaatttta tcaattttaa ctgctgcgtt agtaacagct
     6061 actattattc cttttagtgc cagtgcagca acaactttag gtgatgtcaa tgttgatgga
     6121 aaagtaaatt ccatagacat ggcgattgta aaacaataca tattaggaac aaaaactttt
     6181 tcagatgctg aattgaaggc ggcagatgtc gatgcaaacg gcaaagtaaa cgctatagat
     6241 ttagctttgt acaaacagta catattagga agtataacta agtttccagg agataaaggt
     6301 gtagttgaca atagaactac tacaggacct ggaataacat ggatggatgg ttataaatta
     6361 ttccctgtag gtgtaaacta cggttggaac aattggtgct atgagttttc tgatagcaat
     6421 tgggatacaa actttgctaa gatgaagagc gatatggatg ctatgaattt aaaaggagtt
     6481 aagtctctta gatggtgggt attccctgat cttgcatttg gtccaacttg gtcaggtact
     6541 ggaaaaggaa gcttatgtac tggtcttcca aataattggg taaaccatat gaaggcggtt
     6601 tgtgattacg cacttacaaa aaacattaaa atttattgga cattaactag ttttgatgct
     6661 gcaagacaag atggaacaga tcatcattca gatatattca caaatgaagc tgtaagacaa
     6721 agtttctttg ataatgctgt taaaccaatt gttactgcac ttggaaatca tgaagctgtt
     6781 atgggttggg attgtataaa tgaaccagaa tggatcatca aaaaagagga caatggtgaa
     6841 ccaaaagcca gtggatatga tatatatgct ctttctgata taagaaatta tgttcaatca
     6901 acaacagctt taattcatca gtatgcaaaa caacctgtaa gtgtaggtag tgcaagtatg
     6961 aagtggagtg gagcacaatt tagtctttgg acaggtttag gattagattt ctatgatttc
     7021 cattggtatg attgggcaac accatggttt aatccaatga caacaccagc gtcatcttta
     7081 aagcttgata agccagtaat tataggtgaa atgatgtcag atactttaag ttcttctttg
     7141 aaagttgatc ataaagttgt tatggatgct attatggcta atggatattc aggatatatg
     7201 acttgggcat ggaatgatgg tagttgtgga aactttattg gtagcaattt cactaatttt
     7261 ggagctcaac atactgatat tgttaggtaa aattatttaa taagtttttt tatggtatat
     7321 ttcctacgag tgttatggaa agtctttata acacttgtag gagtaaataa tattttttgc
     7381 aactttttaa atctatttca aaaagttaat gctaattctc agtacaaata taagttttat
     7441 atctatattg attttaccca tataagaatg aagtagctgt aatctttagg gtatatctgt
     7501 atagtttttt gcatattttt aaactctttt atattgggtt tgaaagggtt tataaatata
     7561 aacctactgg agataagaag tatcatacaa caccaaggta gtttatttgg taaaaaagag
     7621 aacaaaaact tggacaccat taactgactg ggtagatttt caggattgga accttcttgg
     7681 ttgtgaaagt atggcagctg accctgttga aactaatcgt gtatacatag ccgttggtac
     7741 atacaacaat gattggacag atatgaatgg cgataaagta gtaaatgtaa ttgattttgc
     7801 attattaaag aaaaaactat tagcttagta ataaaaaaga cgctgctgta gaaatacggc
     7861 agtgtttttg ttgtatagtc ttagaatata gttaactaaa aatacgtgta agtaaatata
     7921 ttcaaaaatt ataaataatt tataaacaaa tttaaaatag gcacctaaat ttaaaaatga
     7981 aaacgaataa ctatagtgga gtgtttattt tacataaaag tgtttatttt tacataataa
     8041 catttaatta ataaaatgta aaatatacag agaaggatga ttaatatata aataatcata
     8101 agtgcattta aagggggagt ataaatgaat agaaaaaaaa taacagcata cgcaactgca
     8161 atagtaacag tactaagtat tccgacagtt gctacttctg ttgtgactag caaatctgtt
     8221 agtgcagcaa cgaattttga ttacagagaa ataatagcaa acaatacatt cgataacaat
     8281 aatgcgacac catggaccct tgttcagaat tatccagcaa tagctaaaat ggtagcaaaa
     8341 gatgggaaat gctctgtaac agtccttaat gatggagtag agggaagatg ggatgttcaa
     8401 ttacgtcaca gaggtatagt tttagaccaa gggcataagt atagagttag attcacagta
     8461 actgctgaca aagattgtta catatatccc aaaataggtg accaaggtga accatataga
     8521 gaatactgga actataactc atatcaaagg atacaattga aagctggtgt tccaacaact
     8581 gttgatcaaa cttttgaaat gaaggatcca acagctaaaa cagctgaatt tgcatttcat
     8641 cttgcaggtg actgtaaagc ggctactttt ccttatacat tcacattcga tgatatttat
     8701 ttaacagacc caaatcgtat accaccaccg ccaccaccag aagagccaac aaatgcggta
     8761 agagttaatc aagttggata ttttacaaat cttcaaaaga aagctactgt ggttactaaa
     8821 gcaactagtc caataacttg gtatctaaaa aacagtgctg gtgttcaagt ggctactggt
     8881 gaaacaaaag ttttgggagc tgatgctgct tcaggtgata atgtgcatat tatagatttc
     8941 tctaattata caaccgaagg ctcagactat acattatcag ttgaccaatc aaatattgat
     9001 tcattaattt gtaattcagc ttcaagttta ccttttgcta taggcaaaga attgtataat
     9061 aaattgcaaa aggatgctat gaaatatttc tacttaaata gaagtggaat tcctataacg
     9121 atgccttatg ctgaacgcac tgatttaact cgtgtagcag gacatacatt agatgtgata
     9181 gcaactgatg caagttcaac tgatccaagt ccttggtata aagaaaatta tagtttagag
     9241 gttacaggtg gatggtatga tgatactaga tataataagt ctgttattaa tggtagtatc
     9301 tctgcttgga caattatgaa tcaatatgaa agagctttaa aagcaaataa ggttagtgtt
     9361 ataccatttg ctgataatac aatgaatatt cctgaaagtg gtaatggata tccagatatc
     9421 cttgatgaag tacgtttcca aatggaaatg atgatgaaga tgcaaatacc agctggtaaa
     9481 acttactctg gtatggttca tcatagggcg aaagatgaga ggtggatacc acttgcaact
     9541 cggccagatc aggaccaaat gaaacgctac ttagagccgc caagtacagc agcgacacta
     9601 gaccttgctg ctacagctgc acaatcttct cgcttatggg caaaatatga ttctacattt
     9661 gctaaccagt gtttggtttc tgctgaaact gcatgggctg cagccattaa gaatccagca
     9721 atatatgcac cacttcaaag tagtttttct ggaacttatg gtgataccaa tatagcagat
     9781 gaattttatt gggcagcagc tgagctttat gaaacaactg gaaagcaaca gtaccttgac
     9841 taccttaaga ataattgtca agataaattc ttaaaaatac caactaaaat agtaggtgga
     9901 gaagccaacg gagtatcagg ttgtttcgac tcgggggatg ttactgggct gggaactatt
     9961 tctcttgcta ttggggataa gttagatgtt gcatcaaaga ctactgcaag agctaatatt
    10021 gcagcagcag ctgatgtttt tgtggctaat acacaaaaag aaggttatgg tactccaatg
    10081 gatcaaggaa ttgtaggaca agtagtggat cagaacggat atgtaacaaa accaatagaa
    10141 ggttatccat atgcatcaaa ttcttatgta actaacgaag ctatagtcat gggttatgct
    10201 tatgacttta caaaaaatag aaattattta aatggtgtag ttagtgcaat ggattatata
    10261 ttagggcgta atccaatggt acaatcctat gttacaggat atggatcaaa tccagttgaa
    10321 aatccacata acgttttttg ggcataccaa tatgacaata catttcctaa ggcaccagca
    10381 ggctgtatgg ccagtggtcc taattcaggt ttacaagatt catgggttaa agcttcaggc
    10441 tggacacctg gaagtagacc agcagctaag tgcttcatgg ataacataga gtcttggtca
    10501 acaaacgact tatcaaatag ctacaatgca tcactggctt gggtttcttc atatgtgaat
    10561 ttaaattcgt taccagaatc aataaaaggt gacattaata atgatgcaaa aattaatgct
    10621 atagatttag ctttgctaaa gaaatatatt ctaaacaatg acataccaat cgttgcaatt
    10681 aatggtgata tcaacggtga tggtatcatt aatgctattg atttattact attaaagaaa
    10741 caactgcttg cataagttta aaaacaaaga agcatctcta taaaatatgg agatgtttct
    10801 ttgtttttgt atagaagttc tatactatat aaaaatgata tattaggata tcaaaggtgc
    10861 tttaagaatt ttttgttaga atttatgctg tttacattta agtatataag attaactttt
    10921 ataacaattt atgaataagt ttaaaaatgc accgaaattt aaaaatgaga acgaataact
    10981 atagcgaagt gtttatttta cataagagtg tttattttta caaattagaa atatttacat
    11041 ggaatgtaaa tagcgcagaa aaagcagttg atatatcaac aattataggt ttatttaaag
    11101 ggggagtata aatgaacaag aaaaaaataa tggcttacgc aacagcgttc gtaacattac
    11161 taagtgttcc aacagttgtt acttctgttg tggctactga atctgttagt gcattcacaa
    11221 ggatcaattt aggggaatta atcagaaata acacatttga ttataatgtt gcaacaccat
    11281 ggcaacttac aaaaatttct ccagcagaag ctagtattga agttaaggat ggaaaatgct
    11341 atgtgacaat acttaaaaat ggtgaagatg gaagatggga tattcaattg cgtcacagag
    11401 gtatagtttt agaccaaggt cataaataca gagttagatt tacagtaact gctgataagg
    11461 attgctatat ctatccaaaa atagctgatc aaggtggaag ttttaatgaa tattggaatt
    11521 ataacacata tcaaaagata caattaaacg ctggtgttcc aacaacaatt gatcaaattt
    11581 ttcaaatgaa caatgcaaca actaggtcag ctgaattcgt atttgatctt gcaggtgatt
    11641 gtcaagcggc tacttttccg tataaactta catttgatga tatttatttg acagatctaa
    11701 accatataca gtactaatac aaccaaaata gcaaatgcgg taagaattaa ttaagttggc
    11761 tatttgatat taaagaagca aatgactgtt taagctgaaa aaaataaaat agaggagtat
    11821 tgctatagaa tgtggcagta ctcctctatt gtgctttaat ggaaaattat aaactgtttt
    11881 acaataatgc attagatagt ggattaatat aaaagatgtt ttggcgattt tacctaacct
    11941 ttttaattaa agaataaaat ataaaggtta ggtatattta tgatagaatc ttcataattt
    12001 aacctgttgt gctagcagat aagttttcat taaaaggata tttataccat gattttaggc
    12061 agtcctatcc tattgtctca tatactgttt ttggaaatta ttatccgaat actaattctt
    12121 taatctttga tatttctata cctatattaa aaaatttatt tataaaatag caaagattat
    12181 gagctaatat tttatttgat attcttgtgg caaatcccca agttgatttt gtaagaaccc
    12241 tctgcatatt taattgctcg gagagctgag aaaaagtagt ttctactcta cgacgagcct
    12301 tgaatatcaa ctgcctaaaa ggttttaaaa gtttagtttt actattgtta cgatttattg
    12361 ttaaaagacg aatgtacctt gtttctttta attgcgaagc aactttttga cctatatatc
    12421 ctttatcacc tattagtata tcaatctctg aattagctgt gagttcccag acgacatctc
    12481 tgtcatcaat atttgctgct gttacagtaa aatctgtgat atagccatcg agggctacta
    12541 aagcatgtaa tttgaatcca taatatgttt cttttttcga agcgcatcgc ccgtaggcag
    12601 cctccggctt aaaagcttta tggaaatgag ctctcccaaa cttacacaca ggaattggca
    12661 tactatctgc aattctcatt cggtcatatt gatagttaag aaatttcgtt aactctttac
    12721 gaatttcatc aatgactcga aataatgact ttctaactct atgaaacctc ggcctactac
    12781 aaaagttggg aaataagtct cgtaggtttt tagagcaaaa tccaaaccat gctttttcag
    12841 agtcaatggt taagagttca cctactaaag aaatcgtaat tatttcgcta tcagtcatta
    12901 ctgatttagc gatgttacga cgatttttaa taaatgttgg agttactttt tggtaaaagt
    12961 catcaattat aacataagtg acaacaataa aatcttttaa gtcatttatt gttatggtag
    13021 aatctttatt aaactctggc atataggtta gcctcctatc attagattag tggtgttttt
    13081 taatgatagg ttagcaaata tgctggagtt tttctatttg taagtattgc cagcgtattt
    13141 aactagcaca acgggttaat ttagagcctc aaggtttaca aatcagtgaa aaagtaatag
    13201 gattagtaaa ggataaaata ttaaagtaat taacgaaact tggtaatttt aatatttata
    13261 taagcttaag aaagggaatt tcccagataa actattattt aaggagagaa aacatggtaa
    13321 taattcaatc aatccaaagt ataatgacta tacttttgat gatttctgtc ggctacatat
    13381 tagataggaa aaaatggttt aatgaagaat ctaaaaaact tttatcaaag cttgtagtaa
    13441 atatttcact tccagcatat atgctgcaaa acatagtaac aaatttcacg agagaaaagc
    13501 tttttgaaat gtcaggtggg ttattaatac catttgtatc aataggaatt tgttatatag
    13561 ttgcagtggt tatagctaag gcaataaagg tacccaaaaa aagatttggg acttttgtaa
    13621 ctttattctt tgtatcaaac acgattttta ttggactacc tgtaaattta gctttatttg
    13681 gagagcagag tataccatat gtattcttgt actatattgc aaatactacg ttcttttgga
    13741 cattaggagt ttattcaata agtaaagatg gtgatgctga agatacgaaa ttatttagta
    13801 tagcatcatt aaaaaaatgc ttttctccgc caatgctagg atttattata ggaatcatat
    13861 ttgttattat aaatcttaaa attccatttt ttattctgga tacatgtaag tatttgggaa
    13921 atttaacaac gcctttgtcg atgcttttta taggagcaac tatggctgct gttgatttaa
    13981 aaacaattag gatagataag gaaatgatat tcttaaatat cggtaggagt gttatttcac
    14041 cacttatagt aataatatta ggattattta taaaaggacc tatgcttatg aaagaagttt
    14101 ttatcataca agcagctatg ccaggaatga taaatatatc aattatttct aaggcatata
    14161 atgcagatta tgaatatgct gcag
//
