LOCUS       AAXF02000054           10317 bp    DNA     linear   BCT 04-AUG-2012
DEFINITION  Bacteroides ovatus ATCC 8483 B_ovatus-MSIQ_Cont457, whole genome
            shotgun sequence.
ACCESSION   AAXF02000054
VERSION     AAXF02000054.1
DBLINK      BioProject: PRJNA18191
            BioSample: SAMN00627058
KEYWORDS    WGS.
SOURCE      Bacteroides ovatus ATCC 8483
  ORGANISM  Bacteroides ovatus ATCC 8483
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Bacteroides ovatus (ATCC 8483)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   3  (bases 1 to 10317)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene:
            X83952) is a member of the division Bacteroidetes. In one
            comprehensive 16S rDNA sequence-based enumeration of the colonic
            microbiota of three healthy adult humans, it represents, on
            average, 0.034% of all 16S rDNA sequences and 0.071% of the
            sequences in its division (Eckburg et. al. (2005)). The sequenced
            strain was obtained from ATCC (ATCC 8483T).
            We have collected 6.9X coverage in plasmid end reads and 454 reads.
            We will be performing one round of automated sequence improvement
            (pre-finishing).
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences.  454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs. These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads.  Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were
            blasted against NCBI'snon-redundant (NR) database and predictions
            generated based on proteinalignments. RNA genes were determined
            using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the
            contig level and may notnecessarily reflect any known order or
            orientation betweencontigs.
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Annotation was added to the contigs in August 2007, and the CDS
            comments were updated in January 2008.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Product names were updated in August 2012.
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Bacteroides ovatus ATCC 8483"
                     /mol_type="genomic DNA"
                     /strain="ATCC 8483"
                     /type_material="type strain of Bacteroides ovatus"
                     /db_xref="ATCC:8483"
                     /db_xref="taxon:411476"
     gene            complement(1..1113)
                     /locus_tag="BACOVA_05488"
     CDS             complement(1..1113)
                     /locus_tag="BACOVA_05488"
                     /EC_number="3.2.-.-"
                     /inference="protein motif:Gene3D:IPR013781"
                     /inference="protein motif:HMMPfam:IPR011683"
                     /inference="similar to AA sequence:REFSEQ:YP_001037824.1"
                     /note="KEGG: bth:BT4668 1.0e-81 arabinogalactan
                     endo-1,4-beta-galactosidase K01224; COG: COG3867
                     Arabinogalactan endo-1,4-beta-galactosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH53"
                     /protein_id="EDO09625.1"
                     /db_xref="InterPro:IPR011683"
                     /db_xref="InterPro:IPR013781"
                     /translation="MIINFNRITMIRKYIMIASALLCGSIFTACDDDNDTPTFPEKTET
                     TYDMSGFARGADVSWLSEMESSGYKFYTSDGKEQECMSLLRDLGINAIRLRVWVNPEND
                     TEDVKGWCNKGDVLLKAWRAHNLGYRLMIDFHYSDRWADPVQQAKPKAWENYTVEELEQ
                     AIADHTKDVLGAIKEMGIDVEWVQVGNETHQGMLFPTGQTTSSDGIKNFAKFINSGYNA
                     VKEIYPNAKVIVHLDQGNDANLYNRIFGYLHDNNAKWDVIGMSLYPDMYPTEGAADDWE
                     DMNNKCIANMKSLIAKYNTPVMMCEIGIPWDYSEAEDFFTDFMTKAKQVEQCLGVFSWE
                     PQSYGGWKNYGKGIFDDSGKPTSAFNAFKR"
     gene            complement(1132..2886)
                     /locus_tag="BACOVA_05489"
     CDS             complement(1132..2886)
                     /locus_tag="BACOVA_05489"
                     /inference="protein motif:superfamily:IPR011050"
                     /inference="similar to AA sequence:REFSEQ:NP_813580.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EDO09626.1"
                     /db_xref="InterPro:IPR011050"
                     /translation="MKKLNNLFTLLSALLLLGACEKDGDTYFLSSPEENELIASANSVV
                     LTKETAKFYALSLAWTDQTLQVSDPRYQPTTGVQTSVQVSRTEDFSGQILETTESGVSK
                     TYSVSALNIVAYQLKATPEEEAPLYFRLAGRNGSNIAPVYSNVVKVMVTPYEIDMRFAN
                     IINKGDGKDSGKDLYAANADGNYIGFMGATSWEGFYLQEADGTVWHTAKSGSTATPFFL
                     TTDAAEGTWDLWFPGQSGCYYTNVNTTKKLWTALWMPTLNVSGIDGGITMEYKRDKNQW
                     QGIFTATGAGTINIQMNGTGKLYDNTSVSGADNSIDDTKAKDTPFAFSGSANELTFNTG
                     TNVSAGNITVNVPAAGECTLIIDLNDPQAWKVEVTEGGSVEPTYPSYLEMQGADSWWGK
                     LYRKLEPGKTAGTYRTVFQTVSNENFQIVDPSTDTWYGSDPNNLYSLALTTGEHYNIWF
                     DGSGKKSYTIEVDYVNLTWKPTEIKQINVYGTFNGWSTAKDLMIYDEETGIWSAECDIT
                     TIGDGFYFLMNTDPDQISWDWRLYYTSESGLYLSDQNGDNIKPTKEGKYRITLDLNKME
                     FTMTEITE"
     gene            complement(2910..4490)
                     /locus_tag="BACOVA_05490"
     CDS             complement(2910..4490)
                     /locus_tag="BACOVA_05490"
                     /inference="protein motif:HMMPfam:IPR012944"
                     /inference="similar to AA sequence:INSD:AAO79775.1"
                     /note="COG: NOG27516 non supervised orthologous group"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8A1G2|8.A.46.1.1"
                     /protein_id="EDO09627.1"
                     /db_xref="InterPro:IPR012944"
                     /translation="MKKIFYTALYFCSALLMSSCIGDLDQYPHEEETSSTIYTSAANYK
                     AVLGKIYAAMVTTGQEKNGDNDLSSNSGQDYMRCFFNLQECGTDEVASTWLSGDNVTGL
                     TYLSWDANDPWVSDMYYRIYYNIALCNEFLRNATDEKIAQFTDQEQTEIRIYRAEARFM
                     RALFYYHALDLFRNIPFVTENDPLVGYLPPRYTAQQIFDYIESELNAIKDEMLNKANCP
                     YGRASQGAAYTLLAKLYLNAEVYTQTSKYNECIKACKKVIEQGYQLESDYYKLFNADND
                     KRTNEIIFTLPVDATNLVSWGSSTYIVCGAVSNTSEKQIPEDYGVKSGWGMFRVRGEIP
                     QLFSGNEEADQRYMFFTDGQTRDLEKIDDQSNGYFVEKWTNLTDAGQTASNTADGGVNT
                     DYPLFRLADVYLMMAEAVVRGGTEATLANVLPYINELQERAFGDNTHNKSESEINELFI
                     INERARELYWEGYRRTDLIRFNQFTTNSYVWQWKGGVKDGKAVESKYNIYPIPATELTA
                     NPNLYNENY"
     gene            complement(4509..7484)
                     /locus_tag="BACOVA_05491"
     CDS             complement(4509..7484)
                     /locus_tag="BACOVA_05491"
                     /inference="protein motif:HMMPfam:IPR000531"
                     /inference="protein motif:HMMPfam:IPR012910"
                     /inference="protein motif:ScanRegExp:IPR002048"
                     /inference="protein motif:superfamily:IPR000627"
                     /inference="protein motif:superfamily:IPR008969"
                     /note="COG: NOG06412 non supervised orthologous group;
                     Psort location: OuterMembrane, score:9.52"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="EDO09628.1"
                     /db_xref="InterPro:IPR000531"
                     /db_xref="InterPro:IPR000627"
                     /db_xref="InterPro:IPR002048"
                     /db_xref="InterPro:IPR008969"
                     /db_xref="InterPro:IPR012910"
                     /translation="MNRNILNLRHLTVLLMLCTAFLGGIIPAFAQQGGKKITGQVIDEN
                     KEPMIGVSILIVGTSTGTVTDFDGNYTLNVPQGSKELQFSYVGYETKVVPVPANSTTLN
                     IQLKSDSQVLSDVVVIGYGTQRKSDLTGSVTNVSSKDFNMGLVSSPEQLINGKISGVQI
                     MSNSGSPTAGSTIRVRGGASLNASNDPLIVLDGVPLEQGGISGNDGNFLSLINPNDIES
                     MTILKDASSTAIYGSRASNGVILITTKKGSSDKLKITFSTTNSVQTRTKLADMLSYDEF
                     VNTIQSKGTDAQRALLGTTHTDWNDEIYQNAFGTDNNLSVAGKITKNLPFRASIGYYNQ
                     SGLLRTDNAERYTGSITLNPSFFKDHLKLNINAKGSINKNTFANTSAIWAASTMNPTIP
                     VYSGLDTFGGYTEAIDNTGAPVNGAVMNPVGLLNMNDSQSTVKRFIGNIDADYRLHFFP
                     DLKLHATLGYDYAQGKGSVYVPAEAAQNYTTSGLDYSYGPQKKENRLLTLYANYNKLVE
                     SIKSSFDVTAGYDYQYWKSTTPLYSEMNTLGEIQKTSKASDERHVLLSYYGRLNYSFNS
                     RYMLTTTVRRDATSRFAKNVRWGTFPSVALGWRVTEESFLKDNKVISTLKVRASYGVTG
                     QQDGIGNYNYLPIYTISQSGAGSLIGDTPTPTYRPKAYVPNLKWETTTSWNMGFDFGFL
                     KDRITGSFDYYTRQTKDLIATVPAAAGTTFDRNITTNVGNVDSQGVEFSLNATPIQTKD
                     WNWDLSFNMTWQKMKVKNLSLTAGTPATNTSVGPYIDSYQVQTLSEGYAPYMFYVYHQL
                     YDEKTGKPIEGAYVDLDGDGQINSNDLYRYHSPAPDYILGFSTSLRYKKWTLSTSLRAN
                     IGNYVYNAMAMNSGAWETVSYNSYQLNNLSSTYLKTGFQTRQYLSDYYVENASFLKMDN
                     LSLGYNFGTIYKGCSLNVSAMVQNVFCITKYSGVDPEIPDGVDNSFYPRPRTFSLNIGI
                     NF"
     gene            7594..7701
                     /locus_tag="BACOVA_05492"
     CDS             7594..7701
                     /locus_tag="BACOVA_05492"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EDO09629.1"
                     /translation="MVQTKDLFLTEEDLVPHRGTTLSSPRNKGFDNGEL"
     gene            complement(7708..10317)
                     /locus_tag="BACOVA_05493"
     CDS             complement(7708..10317)
                     /locus_tag="BACOVA_05493"
                     /inference="protein motif:Gene3D:IPR013781"
                     /inference="protein motif:HMMPfam:IPR001547"
                     /inference="similar to AA sequence:INSD:CAL67108.1"
                     /note="COG: COG3934 Endo-beta-mannanase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH147"
                     /protein_id="EDO09630.1"
                     /db_xref="InterPro:IPR001547"
                     /db_xref="InterPro:IPR013781"
                     /translation="MKRLLILTFICLISAFVKVQGKSSSTPIIYIDGNGVMRWSDTRRE
                     ASFFGVNYTLPFAHAYRAIGYLELDRKAAIDKDVYHISRLGLNAYRIHLWDVELTDGQG
                     NLLENEHLDLMDYLIAKLKERNIHIVITAQTNFGNGYPERNIQTGGFSYKYDKCDMHSH
                     PEAIAAQETYLHGLVKHVNPYTGLAYKDDPSIVGFEINNEPCHSGTKKEVKAYINRMLK
                     AINKTGNRKPVFYNVSHNGYVVEAYYETAIQGTTYQWYPIGLVSGQTQQGNFLPYIDRY
                     DIPFSDKVKGFDKKTRMVYEFDPADIMYSYMYPAMVRTFRTAGFQWITQFAYDPMDIAY
                     ANTEYQTHFLNLAYTPHKAISMKIAAEAARSLKRGESYGSYPQDTLFGDGFRVSYTEDL
                     SELNNGKKFYYSNHTNTQPKDASQLVSIAGCGSSPIIHYEGTGAYFMDCLEPGVWRLEV
                     MPDAVVVNDPFAKPSLDKEVVTIAYGAWDMALQIPDLGMEFTFTALNQGNQQKGDVTDG
                     IIRGLRPGTYLLKRKNCTPKQNWQADSQWNSIRIGEYVAPAPRVTDYKVVHTPSATTEA
                     NKDLTISAQVVGTEFPDSVIIYTDKISFWNEHNPYIKMKRTGGYTYQATIPATEIKDDC
                     FRYNIIVCRGNSTRTYPTGNSGYRNSSLGIKGNPLDWNYTSGAYWTTRVVAPDSAIPLL
                     TITDADSRIEAYTLPEWNDLQRTLVDSSPVEKPLLRFRFTPKGENPHYFLRTFVKNLIE
                     ERKERVKDCSVLCIRVNRTKALPEGFSAGFVTSDGYTYKSPCPAPSSEGIIRIPLKDLR
                     QTDTALLPIAYPTFLKQYFHPETEIAFLPERIEKLELSMSGNKKGLVEIELGNIWLE"
ORIGIN
        1 ttatctcttg aaagcattaa atgcgctggt gggtttacca ctatcatcaa aaatgccctt
       61 tccatagttc ttccaaccac cataactctg cggttcccag gagaatacac caaggcattg
      121 ttccacttgt ttggctttcg tcataaagtc tgtgaaaaag tcttcagctt cggaatagtc
      181 ccagggaata ccaatttcgc acatcatcac cggagtgttg tatttggcaa tcagactttt
      241 catattggca atacatttgt tgttcatatc ttcccaatca tcggctgctc cctctgtcgg
      301 gtacatatcc ggataaagag acataccgat gacgtcccat ttggcattgt tgtcatgcaa
      361 ataaccgaaa atccggttgt atagattggc atcatttccc tggtcgaggt gtacaatcac
      421 tttcgcattc ggataaatct ctttaacagc attgtagccg gagttaatga atttcgcaaa
      481 gtttttaata ccgtcactac tcgttgtttg tccagtgggg aatagcattc cctgatgagt
      541 ctcattgcct acttgcaccc attccacgtc aatgcccatc tctttgattg caccgagtac
      601 atctttggta tggtcggcaa tcgcctgttc cagttcttct accgtatagt tttcccaggc
      661 tttgggtttg gcttgttgaa caggatcggc ccatctgtca ctgtaatgga agtcgatcat
      721 caggcgatag cccaaattat gcgctctcca tgctttgagc aatacgtcac ccttattgca
      781 ccagcctttt acatcttccg tgtcattttc cgggttcacc catacacgta gacgaattgc
      841 atttataccc aggtcgcgaa gcagagacat acattcctgt tcttttccat cggaggtata
      901 gaatttatat ccggaacttt ccatttccga cagccaactg acatcagctc cccgggcgaa
      961 gccggacata tcataagtgg tttccgtctt ttccggaaaa gtcggagtat cattatcatc
     1021 atcacaagcg gtgaagatgc ttccgcaaag tagtgcggaa gctatcatta tatattttcg
     1081 tatcatagtg attctgttga aattaataat catgttgttg aacttagaat gttattcagt
     1141 gatttcagtc attgtaaact ccatcttgtt gagatccagc gtaatgcgat atttaccctc
     1201 cttagtgggt ttaatgttat caccattctg atcacttaga tagagcccac tttcagaagt
     1261 atagtacaat ctccagtccc agctaatctg atcgggatcc gtattcatga ggaagtagaa
     1321 tccgtcaccg atggtggtga tatcgcattc tgcgctccat attccggttt cctcatcata
     1381 tatcatcagg tctttagccg ttgaccagcc attgaaagtt ccatatacat tgatttgttt
     1441 gatttcagta ggcttccagg ttaagttgac ataatctact tcgattgtat aacttttctt
     1501 gccggaacca tcgaaccaga tgttgtaatg ctctcctgtt gtaagagcaa ggctataaag
     1561 attgttggga tcagagccat accatgtgtc tgttgatggg tctactatct ggaagttttc
     1621 attgctgacg gtttggaata cagtgcgata agttcccgca gttttaccgg gttcaagttt
     1681 gcggtataac tttccccacc atgagtcggc accttgcatt tccagataag agggataagt
     1741 cggttctaca cttcctcctt ccgtcacttc cactttccat gcttgcgggt cgttcaggtc
     1801 aataatcaat gtacattctc cggctgctgg tacgtttact gtaatatttc cggcagaaac
     1861 attcgtcccg gtattgaatg tcaattcatt agcagaacca ctaaaggcga aaggagtatc
     1921 tttggctttc gtatcatcaa tgctgttatc agctccgctg acgctggtgt tatcatatag
     1981 tttacctgtt ccattcatct gaatattgat agtgccggct cctgtagcag taaatatgcc
     2041 ctgccattga ttcttgtcac gtttgtattc catcgtgata cctccgtcga tgccacttac
     2101 attgagtgtt ggcatccata aggcagtcca tagttttttg gtggtattga cattcgtata
     2161 gtaacagccc gattgaccgg ggaaccacaa atcccacgtt ccttcagcag cgtcagttgt
     2221 caggaagaac ggagtagccg tactgcccga tttggctgtg tgccaaacag taccatcggc
     2281 ttcctgtaga tagaaacctt cccaagaagt ggctcccata aagccgatat agttgccatc
     2341 cgcattggct gcatacagat cttttcctga atccttgccg tctcccttat tgataatatt
     2401 ggcaaatcgc atatcaattt cataaggagt aaccatgact tttaccacat tactgtatac
     2461 aggagcgatg ttacttccgt ttcttccggc aagccggaaa tagagcggcg cttcttcttc
     2521 gggagtagct ttcaactgat aagcgacgat atttaaagcg gagacagagt acgtctttga
     2581 tactccactt tcggtcgttt ccagaatctg cccggagaaa tcttccgtgc gtgaaacctg
     2641 tacggaagtt tgtacaccgg tggtaggctg gtatcttgga tcgctcactt gcagcgtctg
     2701 gtcagtccag gcaagcgaca aagcataaaa cttggctgtc tcctttgtca gtacgacaga
     2761 attggcagaa gctatcaatt cgttctcttc cgggctggag agaaaatagg tatccccatc
     2821 tttctcgcaa gcacctagta ataggagggc ggaaagtaaa gtgaatagat tatttagttt
     2881 tttcatatcg ttgttccgta ttaatatgat taatagtttt cattgtataa gtttggattg
     2941 gccgtcagtt cggtagcagg tatcggatag atattgtatt tgctctctac tgccttaccg
     3001 tcttttacgc cacctttcca ttgccataca tagctgttgg ttgtaaattg attgaagcgg
     3061 atcaagtccg tacggcgata cccctcccaa tacagttcac gggcacgttc gttgatgata
     3121 aacagttcgt tgatttcaga ctcgcttttg ttatgagtgt tgtcgccgaa agcacgttcc
     3181 tgtagctcat tgatataagg cagtacattt gccagagtcg cttccgttcc tccacgtacc
     3241 acagcttctg ccatcattaa atatacatcg gccagacgga ataggggata atctgtattt
     3301 acaccaccgt ctgcggtgtt ggaagcagtc tgtccggcat cagtcaggtt agtccatttt
     3361 tctacgaaat agccattgct ttggtcatct atcttttcca ggtcccgggt ttgtccgtcg
     3421 gtaaagaaca tatatcgctg gtcggcttct tcgttaccgg agaaaagttg aggaatttct
     3481 ccgcgtacac ggaacatacc ccatcctgat tttacaccgt aatcttccgg tatctgtttt
     3541 tcggacgtgt tgctcactgc gccacaaaca atgtaagtgg atgatcccca tgacacgaga
     3601 ttggttgcgt ctaccgggag ggtgaagatt atttcgttcg tacgtttgtc attgtcggca
     3661 ttaaacagct tgtaataatc gctttccagt tgataaccct gttcgataac ctttttacag
     3721 gctttgatac attcgttgta tttggaagtc tgggtataaa cttccgcatt cagatataat
     3781 ttggcaagta atgtatatgc agctccttgg gaagcgcgtc cgtaaggaca gttcgcttta
     3841 tttagcattt catctttaat ggcattcagt tcactttcaa tataatcgaa aatctgttgg
     3901 gctgtatagc ggggaggcag atatccgact agcgggtcat tttccgtcac aaacgggata
     3961 ttgcggaaca gatcgagtgc gtgatagtag aataaagcac gcatgaaacg ggcttcggca
     4021 cgataaatac gaatttcagt ctgctcctgg tccgtgaact gtgctatctt ttcatcggtg
     4081 gcattgcgga gaaactcatt acataaagca atgttgtaat agatacggta atacatatcc
     4141 gatacccaag gatcattagc gtcccaactc aaatacgtca gtcccgttac gttgtcaccg
     4201 gacagccagg tagaagccac ttcatccgtt ccacattcct gcaagttgaa gaaacagcgc
     4261 atataatcct gtccgctgtt actactcaaa tcgttgtcac cattcttttc ctgtccggtg
     4321 gtcaccatgg ctgcatagat ttttcccaga acggctttat agtttgcagc actggtatat
     4381 atggtagaag atgtttcttc ttcgtgtgga tattggtcca agtcgcctat gcaggaactc
     4441 atcaggagtg cgctgcaaaa ataaagggct gtataaaata tctttttcat aatcgatgct
     4501 tttagtaatt agaagttaat acctatgttg agagagaaag tacgtgggcg tggataaaac
     4561 gaattatcca caccgtcggg aatttccggg tcaacgccgc tgtatttggt gatacagaat
     4621 acattttgca ccatggcact cacattcaga ctgcatcctt tataaatagt gccgaagttg
     4681 taacccaatg agagattatc cattttcagg aaagaagcat tctctacata gtagtcagag
     4741 agatattgac gtgtctggaa tcctgttttc agataggttg atgacaggtt gtttagctga
     4801 taagagttat agctgacagt ttcccaggca ccgctgttca tagccatcgc attatagaca
     4861 tagtttccga tattggcacg caggctggtg ctcaatgtcc acttcttgta acgcaatgag
     4921 gtactgaaac cgaggatata gtcgggagcc ggagagtggt agcggtacaa gtcgttcgag
     4981 ttgatttgtc cgtccccgtc aaggtctacg taagctcctt ctatcggttt gccggttttt
     5041 tcatcataca gctgatggta aacgtagaac atataaggag catatccctc gctcaatgtc
     5101 tgtacctgat aactgtcgat atacggacct acagaagtgt tggtggcagg cgtaccggct
     5161 gtcagtgata agtttttcac tttcatcttc tgccatgtca tgttgaaact caagtcccag
     5221 ttccagtcct tggtctgtat gggcgttgcg ttcagactga attccacgcc ctgactgtcc
     5281 acatttccga cattggtcgt gatgttacga tcgaatgtag taccggcagc agcaggtacg
     5341 gtggcaatca ggtctttggt ctgacgggtg tagtagtcga aactaccggt gatacggtct
     5401 ttgaggaaac cgaaatcaaa acccatattc catgaagtgg ttgtttccca tttcaggttg
     5461 ggtacatacg ctttggggcg gtaagtagga gtcggggtat ctccgataag ggaaccggca
     5521 ccactttggc tgatcgtata gatagggagg tagttgtaat tgcctatacc gtcttgctgc
     5581 ccggtgacac cataactggc acgcactttc agcgtactga taactttatt gtctttcagg
     5641 aatgattctt ctgtcactct ccatcctaat gctactgacg ggaacgtacc ccaacgtaca
     5701 tttttagcga agcgcgaagt ggcatcgcga cgtacagtag tagtcagcat atagcgggag
     5761 ttgaacgagt agttcaaacg gccataataa gagagtaata catggcgttc gtcagaggct
     5821 ttcgatgttt tctgtatttc tcccaacgtg ttcatttcgc tataaagcgg agtcgtggat
     5881 ttccagtact gataatcgta accggctgtt acatcaaaac tactcttaat ggattccacc
     5941 aatttgttgt agttggcata cagagtcaac agacggtttt cttttttctg tgggccatag
     6001 ctgtaatcca aaccggaagt ggtgtaattc tgtgcagctt ctgccggaac atatacactt
     6061 cctttacctt gtgcatagtc ataacctaac gtagcatgca acttcaaatc ggggaagaag
     6121 tgaagacgat agtctgcatc aatgtttccg ataaaacgtt ttactgtact ttgtgagtca
     6181 ttcatattga gaagtcccac cggattcatg actgctccgt tcacaggagc accggtgttg
     6241 tcaattgctt ccgtatatcc gccgaatgtg tctagacccg aatatactgg aatggtagga
     6301 ttcatggtcg aagcagccca aatggcactt gtattggcaa atgtgttctt attgatagaa
     6361 cctttagcat tgatattcag ttttaagtgg tctttaaaga aagacggatt caatgtaatg
     6421 cttccggtgt aacgctccgc gttgtcagta cgtaataaac cactctggtt gtagtaaccg
     6481 atagatgcac ggaaaggtaa gtttttggta atctttccag caacacttag attattatcc
     6541 gtaccgaagg cattttgata aatctcatcg ttccagtccg tatgtgtcgt gccgagtaac
     6601 gcccgttgtg catcggttcc tttgctttgg atggtattga caaattcatc gtaactcaac
     6661 atatctgcca atttggtacg ggtttgtaca gagttggtcg tactgaatgt aatcttcagt
     6721 ttatccgaac ttcctttctt ggtagtaatc agaataaccc cgttggatgc gcgcgaacca
     6781 taaatggcag tcgaagaagc atctttcaga atggtcatac tttcaatatc gttcgggttg
     6841 atgaggctaa ggaaattccc gtcattaccg ctgatgcctc cctgttccaa aggaacgccg
     6901 tccagtacga tcaaagggtc attactagcg ttcagtgaag caccaccacg cacacggatc
     6961 gtacttcccg cagtgggaga accactgttg gacatgattt gcacaccgga aatcttacca
     7021 ttaatcagtt gctcgggaga gctgaccaat cccatgttga agtccttgct gctgacgtta
     7081 gttaccgaac ctgtcaggtc gcttttacgc tgcgtaccat agccgattac tacaacgtcg
     7141 ctcaatacct gtgaatcact tttcaactgg atattcaaag tagtactgtt ggcaggaact
     7201 ggtactacct ttgtttcata tcctacataa gagaattgca actctttgct gccttgtggc
     7261 acattgagtg tataattacc gtcgaaatcg gtcactgttc cggtagaagt accgacaatg
     7321 aggatactta ctccgatcat cggttccttg ttctcgtcaa tcacttgtcc ggtgattttc
     7381 ttgcctcctt gttgggcgaa ggcaggaatg atgcctccta aaaaggctgt acacaacata
     7441 agtagtacag tcagatggcg tagatttaag atattcctgt tcataattac tgtgttattg
     7501 ataatagatt tatttttact ttttatttaa aaatcagcgg cttttctctt tctgtccctt
     7561 ccgtttgcta cctcctttca gaaggcttca gacgtggttc agacgaaaga tttgtttctc
     7621 accgaggaag accttgttcc tcaccgagga acgacccttt cctcaccgag gaacaaaggc
     7681 ttcgacaacg gggaacttta agaatgatta ttccagccag atatttccta gttcaatctc
     7741 aactaaacct ttcttgtttc ctgacattga cagctccagc ttctcaatcc tttccggtaa
     7801 aaatgcgatt tccgtttccg gatggaaata ctgtttcaag aatgtcggat aagctatcgg
     7861 cagcagcgcc gtgtctgtct ggcgtaaatc tttcaacggt atccgtatga ttccctccga
     7921 tgatggagca ggacaagggc ttttataagt atatccgtct gacgttacga aaccggcact
     7981 gaatccttcc ggaagtgctt tcgtgcgatt tacccggata cagagaacgc tacaatcctt
     8041 tactctttct tttctttctt caatcaggtt ctttacgaac gtgcggagga aatagtgcgg
     8101 attttctcct ttaggagtaa accggaaccg gagcaaaggt ttttccaccg gagaagaatc
     8161 aaccaaagta cgttggagat cattccattc gggaagcgtg tatgcttcta tccggctatc
     8221 tgcgtccgta atagtcaata gtggtatcgc actgtctggg gcaacaacac gagtagtcca
     8281 ataggctccg gaagtataat tccagtccag tggatttccc tttattccca atgatgaatt
     8341 cctgtaaccg gaatttcctg tgggataagt gcgagtgctg ttgccccggc atacgatgat
     8401 gttatacctg aaacaatcat cctttatttc tgtagcgggt atcgttgctt gataggtata
     8461 tccgcctgta cgtttcatct tgatatatgg attatgctca ttccaaaaag atatcttatc
     8521 ggtgtagata atcacagaat ccgggaattc ggttcctacc acctgtgcac taatcgtcaa
     8581 atccttattt gcttcagtgg ttgccgatgg agtatgaacg actttataat ccgtcacacg
     8641 tggagcagga gccacatact ctccgatgcg gatggaattc cattgagagt ctgcctgcca
     8701 gttctgtttg ggcgtacagt ttttacgttt cagcagatag gtacccggac gaagtccgcg
     8761 gataatcccg tctgtcacat ctcctttctg ctgatttcct tgattgaggg ccgtaaaggt
     8821 aaattccatc cctaaatcag gtatttgcaa agccatgtcc caagcaccat aagcgatggt
     8881 aaccacttct ttatcgagtg acggtttggc aaacggatcg ttcactacta ccgcgtccgg
     8941 catcacttcc agtctccaga ctcccggttc gaggcaatcc atgaaataag cacctgttcc
     9001 ctcataatga atgatcggag aactgccaca accggcaatg gaaacaagtt gtgacgcatc
     9061 tttgggctgg gtgttggtat gattggaata atagaatttc tttccattat ttaattcgct
     9121 caagtcctct gtataactga cacgaaaacc gtcaccaaat aaagtgtcct gtggataact
     9181 gccgtatgat tcacctcttt tcagactgcg tgcagcttcg gcagctatct tcatactgat
     9241 ggctttatga ggcgtatagg caagattcaa gaaatgagtc tgatattccg tattggcata
     9301 agcgatatcc atcggatcat acgcaaactg ggtaatccat tgaaaacctg ctgtacggaa
     9361 agtgcgtacc atggccggat acatataaga atacatgata tctgccggat cgaactcata
     9421 taccatccgt gttttcttat cgaatccttt cactttatcg gaaaagggaa tatcatagcg
     9481 gtctatatag ggaaggaaat ttccctgctg tgtctgtcca gataccagtc cgatgggata
     9541 ccattgataa gtagttccct gaatggcagt ttcgtaataa gcctctacta catatccgtt
     9601 gtggcttaca ttatagaata caggtttgcg gtttcctgtt ttattgatcg ctttcagcat
     9661 ccggttgata taggctttta cctctttctt cgtaccggaa tgacaaggtt cattattgat
     9721 ttcgaaccca acaatggagg gatcgtcttt ataagctaac cccgtatatg gattgacgtg
     9781 cttcaccaaa ccatgaagat aggtttcttg tgcagcgata gcttccgggt gggagtgcat
     9841 atcgcattta tcatatttat aggagaagcc gcccgtctgt atgtttcgtt cgggatatcc
     9901 gttcccgaag ttggtctgcg cggtaatcac aatatgaata ttgcgttctt tgagtttggc
     9961 aatcaggtag tccatcaagt cgagatgctc gttttcgagg agatttccct gtccgtctgt
    10021 cagttccaca tcccataaat gaatacggta agcattcagt cccagacggg agatatgata
    10081 tacatcttta tctatggcag ctttgcggtc cagttccaaa tatccaatgg cacggtaagc
    10141 atgggcgaaa ggcagcgtat aattaacacc gaaaaaggaa gcctcccggc gggtgtcact
    10201 ccaacgcatc accccatttc catctatata tataataggt gtagaggatg atttgccttg
    10261 cactttgaca aaggcagata tcaaacaaat aaaggtaagg atcaacagtc ttttcat
//
