LOCUS       AF039487                4846 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Thermotoga neapolitana cellobiose phosphorylase (cbpA) and
            beta-glucan glucohydrolase (gghA) genes, complete cds; and putative
            amylase B (amyB) gene, partial cds.
ACCESSION   AF039487
VERSION     AF039487.2
KEYWORDS    .
SOURCE      Thermotoga neapolitana
  ORGANISM  Thermotoga neapolitana
            Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Yernool,D.A., McCarthy,J.K., Eveleigh,D.E. and Bok,J.D.
  TITLE     Cloning and characterization of the glucooligosaccharide catabolic
            pathway beta-glucan glucohydrolase and cellobiose phosphorylase in
            the marine hyperthermophile Thermotoga neapolitana
  JOURNAL   J. Bacteriol. 182 (18), 5172-5179 (2000)
   PUBMED   10960102
REFERENCE   2  (bases 845 to 3262)
  AUTHORS   Yernool,D.A., Bok,J.D., McCarthy,J.K., Sullivan,R.F. and
            Eveleigh,D.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-1997) Biochemistry and Microbiology, Cook College,
            Rutgers University, 309 Lipman Hall, New Brunswick, NJ 08903-0231,
            USA
REFERENCE   3  (bases 1 to 4846)
  AUTHORS   Yernool,D.A., Bok,J.D., McCarthy,J.K., Sullivan,R.F. and
            Eveleigh,D.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2000) Biochemistry and Microbiology, Cook College,
            Rutgers University, 309 Lipman Hall, New Brunswick, NJ 08903-0231,
            USA
  REMARK    Sequence update by submitter
COMMENT     On Mar 3, 2000 this sequence version replaced AF039487.1.
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Thermotoga neapolitana"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:2337"
     gene            461..2910
                     /gene="cbpA"
     regulatory      461..464
                     /regulatory_class="ribosome_binding_site"
                     /gene="cbpA"
     CDS             472..2910
                     /gene="cbpA"
                     /EC_number="2.4.1.20"
                     /function="phosphorolysis of cellobiose beta-1,4 linkages"
                     /note="CbpA"
                     /codon_start=1
                     /transl_table=11
                     /product="GH94"
                     /protein_id="AAB95491.2"
                     /translation="MKFGYFDDKNREYVIVTPRTPYPWINYLGTEDFFSIISHMAGGYC
                     FYKDARLRRITRFRYNNVPTDAGGRYFYIREEDGDFWSPTWMPVRRDLSFFEARHGLGY
                     TKIAGERNGLRATITFFVPRHFTGEVHHLVLQNRTERPRRIKLFSFIEFCLWNALDDMT
                     NFQRNYSTGEVEIEGSVIFHKTEYRERRNHYAFFSVNHSIDGFDTDRESFMGLYNGFEA
                     PQAVVEGNPRNSVASGWAPIASHYLELEIPPLGEKELIFILGYVENPEEEKWERPGVIN
                     KKRAKEMIERFKTGEDVERALKELKEYWDELLGRIQVETHDEKLNRVNIWNQYQCMVTF
                     NIARSASYFESGISRGIGFRDSNQDILGFVHMIPEKARQRILDLASIQFEDGSTYHQFQ
                     PLTKKGNNEIGGGFNDDPLWLILSTSAYIKETGDWSILNEEVPFDNDPDKKATLFEHLK
                     RSFYFTVNNLGPHGLPLIGRADWNDCLNLNCFSKNPDESFQTTVNALDGRVAESVFIAG
                     LFVLAGKEFVEICRRLGLEDEAKEAEKHVKKMIETTLEYGWDGEWFLRAYDAFGRKVGS
                     KECEEGKIFIEPQGMCVMAGIGVENGYAKKALDSVKEHLDTPYGLVLQQPAYSRYYIEL
                     GEISSYPPGYKENAGIFCHNNPWVAIAETVIGRGDRAFEIYRKITPAYLEDISEIHRTE
                     PYVYAQMVAGKDAPRHGEAKNSWLTGTAAWSFVAITQYILGVRPTYDGLMVDPCIPEDW
                     DGFKITRRFRGATYEITVKNPHHVSKGVKEIIVDGKKIEGQVLPVFNDGKVHRVEVLMG
                     "
     gene            3001..4347
                     /gene="gghA"
     regulatory      3001..3004
                     /regulatory_class="ribosome_binding_site"
                     /gene="gghA"
     CDS             3013..4347
                     /gene="gghA"
                     /EC_number="3.2.1.21"
                     /function="hydrolysis of cellobiose beta-1,4 linkages"
                     /note="GghA; beta-glucosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q86Z14|8.A.49.1.2"
                     /protein_id="AAB95492.2"
                     /translation="MKKFPEGFLWGVATASYQIEGSPLADGAGMSIWHTFSHTPGNVKN
                     GDTGDVACDHYNRWKEDIEIIEKIGAKAYRFSISWPRILPEGTGKVNQKGLDFYNRIID
                     TLLEKNITPFITIYHWDLPFSLQLKGGWANRDIADWFAEYSRVLFENFGDRVKHWITLN
                     EPWVVAIVGHLYGVHAPGMKDIYVAFHTVHNLLRAHAKSVKVFRETVKDGKIGIVFNNG
                     YFEPASEREEDIRAARFMHQFNNYPLFLNPIYRGEYPDLVLEFAREYLPRNYEDDMEEI
                     KQEIDFVGLNYYSGHMVKYDPNSPARVSFVERNLPKTAMGWEIVPEGIYWILKGVKEEY
                     NPQEVYITENGAAFDDVVSEGGKVHDQNRIDYLRAHIEQVWRAIQDGVPLKGYFVWSLL
                     DNFEWAEGYSKRFGIVYVDYNTQKRIIKDSGYWYSNGIKNNGLTD"
     gene            4558..>4846
                     /gene="amyB"
     regulatory      4558..4561
                     /regulatory_class="ribosome_binding_site"
                     /gene="amyB"
     CDS             4571..>4846
                     /gene="amyB"
                     /note="AmyB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative amylase B"
                     /protein_id="AAF37330.1"
                     /translation="MRKCLVLFLLTLTAVLFATSWHDVVLYEIMIDRFYDGDPMNNDQG
                     YGEYDPSNPSKYSGGDLKGIIEKLDYIKGLGVDGIWITPPVANQWWD"
ORIGIN
        1 tgagtagtgg tacgtcaatc ggaaatctta cctgcaactg ttcaatatca aaggatgggt
       61 gtctgtgaga tacacaggaa cttttgttat gttcgttcct gtggtgaaaa gcacacatcc
      121 agatatcagt cccagaaaca aaactgccca gagagcgtac ttcatggttt cacctccttt
      181 cgttgaagta ttttaactcc agattagcaa aaagtcaatt ttgagcaacg aaatgttttt
      241 cttgtgagaa taaatgtctt tttaaaaagc acattttgtc atctcaataa actaacaaca
      301 ttgaatttgt ataaaccatc cagaaattaa acaaagcaga atcagaagaa aatggtttct
      361 tttcggacaa tattcatcga tacttgctcg tggctttcct tccttggatt ttgaatgaaa
      421 acattttcac attagaatac tcacgataaa aagcctcaga gaggtgatag catgaagttc
      481 ggctacttcg atgacaaaaa cagagaatac gtcatagtaa ctcccaggac accctatccg
      541 tggataaatt acctggggac agaagatttc ttttccatca tctcccacat ggcggggggc
      601 tactgtttct ataaagatgc aaggctcaga aggatcacca ggttcaggta caacaacgtg
      661 ccaacagacg ctggaggaag gtatttttac ataagagaag aagacggaga tttctggtct
      721 cccacctgga tgccggtgag gagagatctt tcgttctttg aagcaagaca cggccttggg
      781 tacacgaaga tcgctgggga gagaaacggt ctgagagcga ccatcacctt ttttgttcca
      841 aggcatttca caggagaggt ccatcatctt gtacttcaaa acagaacgga acgaccaaga
      901 aggatcaaac tcttttcctt catagagttc tgtctctgga acgccctcga tgacatgacg
      961 aactttcaga gaaactacag caccggagaa gtggagatag aaggttctgt gatattccac
     1021 aaaacagagt acagagaaag aagaaaccac tacgcgttct tctccgtgaa ccattcgata
     1081 gatggtttcg acacagacag ggagtcattc atgggtcttt acaacggttt tgaggctcct
     1141 caggcggttg tggagggcaa tcccagaaac tccgtggcga gtggctgggc acccattgca
     1201 tctcactatc ttgaacttga gatcccacct cttggcgaaa aagaactcat cttcatactc
     1261 ggctacgtag aaaatcccga agaggaaaaa tgggaaagac ccggggtgat aaacaaaaag
     1321 cgtgcaaaag aaatgataga aaggttcaaa acaggtgaag acgttgaacg cgctctgaag
     1381 gaactgaagg aatactggga tgaacttctt ggaagaatac aggtggaaac acacgatgaa
     1441 aaactcaaca gggtgaacat ctggaaccag tatcagtgca tggtcacttt caacatcgcg
     1501 cggagcgctt cctactttga atccggaatc agcaggggta taggtttcag agattcaaat
     1561 caggatatac ttggatttgt ccacatgata ccggaaaagg caagacagag gattctcgac
     1621 cttgcctcca tccagtttga agacggaagc acctatcatc agttccagcc gctcacaaaa
     1681 aagggaaaca acgaaatcgg tggaggattc aacgacgatc ccctctggct cattctctca
     1741 accagtgcgt acatcaagga aacgggagac tggagcattc tgaacgaaga ggttccgttt
     1801 gacaacgatc cagacaaaaa ggcaacgctt tttgaacacc tgaaaagatc tttctacttc
     1861 accgtgaaca acctcggtcc acatggtctt ccactcatag gaagggcgga ctggaacgac
     1921 tgtctcaacc tgaactgttt ttccaaaaat ccagatgaat ccttccagac aaccgtcaac
     1981 gccctcgatg gaagggtagc agaatccgtc ttcatagcag gactgttcgt tctggctgga
     2041 aaagagttcg tggagatctg cagaagactc ggactcgagg atgaagcaaa agaggcagaa
     2101 aaacacgtga agaaaatgat agagaccact ctggagtacg gctgggacgg agagtggttc
     2161 ctcagggcgt acgatgcctt tggaaggaaa gtgggaagta aagaatgtga agaagggaag
     2221 atcttcatag aaccacaggg aatgtgcgtg atggcgggaa taggagtgga aaacggatac
     2281 gcaaagaaag cacttgattc agtgaaagag cacctcgaca caccttacgg ccttgttctt
     2341 cagcagcccg cttacagcag atactacata gaactaggtg agatctcgag ttatccaccg
     2401 ggctacaagg aaaacgctgg tatattctgt cacaacaatc cgtgggtagc gatagcagaa
     2461 actgtcattg gaagaggaga cagagccttt gagatctaca gaaagatcac tcccgcctat
     2521 ctggaagaca taagtgaaat ccacagaaca gagccctacg tctacgcaca gatggtggca
     2581 ggaaaagatg caccaagaca cggagaagcc aaaaactcct ggctcacagg aacagcggca
     2641 tggagctttg ttgcgataac tcagtacatt ctgggagtaa ggcccaccta cgatggcctc
     2701 atggtggacc cgtgcatacc tgaagattgg gacggcttca aaatcacaag aaggttcagg
     2761 ggagcaacct acgagataac ggtgaaaaat ccccaccacg tttccaaagg tgtaaaggag
     2821 ataatcgtcg acggaaagaa aattgaggga caggtcctgc cagttttcaa cgatggaaaa
     2881 gtgcacagag tagaagttct gatgggatga tcttccctca tgtttcaaac tttgaagaag
     2941 gggccatccc cttctttttt tggggtacaa tagttcggca aacaaactac agcaaagagc
     3001 gaggtgatga tcgtgaaaaa gtttcccgaa gggttcctct ggggagttgc gacagcttcc
     3061 taccagatcg aaggctctcc ccttgcagac ggagcaggta tgtccatctg gcacaccttc
     3121 tcccatacac ccggaaacgt gaaaaacggt gacactggag atgtggcctg cgatcactac
     3181 aacagatgga aagaggacat cgagatcatc gaaaaaatcg gtgcaaaggc ctacagattc
     3241 tccatcagct ggccaaggat ccttccggaa ggaacgggaa aagtgaacca gaaaggtctg
     3301 gacttctaca acaggatcat agacaccctc ctggagaaga acataacacc atttataacc
     3361 atctaccact gggaccttcc cttttctctg cagttgaagg gtggctgggc aaatcgggac
     3421 atagccgact ggttcgcaga gtactcaagg gttctctttg aaaacttcgg tgaccgcgtc
     3481 aaacactgga tcactttgaa cgaaccctgg gtcgtcgcta tagtgggaca tctgtacgga
     3541 gtccatgccc ctgggatgaa agacatctac gtagcattcc atacggttca caacctcttg
     3601 agagcgcacg caaaatccgt gaaggtgttc agagaaacgg tgaaagacgg aaagataggg
     3661 attgttttca acaacggata cttcgaacct gcaagtgaga gagaagaaga tatcagagcc
     3721 gccagattca tgcatcagtt caacaactac ccgctctttt tgaaccccat ctacagggga
     3781 gaatacccgg atcttgtcct ggaatttgca agagagtacc tccccagaaa ctatgaggat
     3841 gacatggaag agataaaaca ggagatcgac ttcgttgggc tgaactacta ttcaggtcac
     3901 atggtgaaat acgatccgaa ctcaccggcc agggtgtcct tcgtcgaaag aaaccttccc
     3961 aaaaccgcca tgggatggga gatcgttcct gagggtatct actggattct caaaggtgtc
     4021 aaagaagagt acaacccaca ggaggtgtac atcacagaga acggagctgc attcgacgac
     4081 gtggtaagtg agggcggaaa agttcacgac cagaacagga tagattactt gagagcccac
     4141 atcgaacagg tctggagagc aatccaggat ggagtcccac tgaagggtta cttcgtgtgg
     4201 tcactccttg ataacttcga gtgggcggaa ggatattcga agaggttcgg tatagtgtac
     4261 gtggactaca acacacagaa gaggatcata aaagacagtg gatactggta ctcgaacggg
     4321 atcaaaaaca acggactaac agattgaaaa gggcggagca atccgccctt tctttttgat
     4381 acctcatttt ccttcctatc tgtccatccc gggcgattat ccccgattat tttcatttaa
     4441 cgaaaaaaat ctcctgtttt ctcccgatat ctcgtttgca ttgaaactat gagtctggca
     4501 aatatctatt aggttaattt gattataccg tcagttaact caaccacacc actgaaggag
     4561 ggatatttca atgagaaagt gtttggttct gtttcttttg actctaactg ctgttttatt
     4621 tgctacttct tggcatgacg ttgtattgta tgaaatcatg atagacagat tctacgacgg
     4681 tgatcctatg aacaatgatc aaggttatgg tgagtatgat ccaagtaatc catcaaaata
     4741 tagcggagga gatttgaaag ggataataga gaaattagat tacataaagg ggctaggggt
     4801 cgatggaatt tggatcaccc caccagttgc taaccaatgg tgggat
//
