LOCUS       AAXF02000047           14774 bp    DNA     linear   BCT 04-AUG-2012
DEFINITION  Bacteroides ovatus ATCC 8483 B_ovatus-MSIQ_Cont517, whole genome
            shotgun sequence.
ACCESSION   AAXF02000047
VERSION     AAXF02000047.1
DBLINK      BioProject: PRJNA18191
            BioSample: SAMN00627058
KEYWORDS    WGS.
SOURCE      Bacteroides ovatus ATCC 8483
  ORGANISM  Bacteroides ovatus ATCC 8483
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 14774)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Bacteroides ovatus (ATCC 8483)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14774)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   3  (bases 1 to 14774)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene:
            X83952) is a member of the division Bacteroidetes. In one
            comprehensive 16S rDNA sequence-based enumeration of the colonic
            microbiota of three healthy adult humans, it represents, on
            average, 0.034% of all 16S rDNA sequences and 0.071% of the
            sequences in its division (Eckburg et. al. (2005)). The sequenced
            strain was obtained from ATCC (ATCC 8483T).
            We have collected 6.9X coverage in plasmid end reads and 454 reads.
            We will be performing one round of automated sequence improvement
            (pre-finishing).
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences.  454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs. These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads.  Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were
            blasted against NCBI'snon-redundant (NR) database and predictions
            generated based on proteinalignments. RNA genes were determined
            using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the
            contig level and may notnecessarily reflect any known order or
            orientation betweencontigs.
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Annotation was added to the contigs in August 2007, and the CDS
            comments were updated in January 2008.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Product names were updated in August 2012.
FEATURES             Location/Qualifiers
     source          1..14774
                     /organism="Bacteroides ovatus ATCC 8483"
                     /mol_type="genomic DNA"
                     /strain="ATCC 8483"
                     /type_material="type strain of Bacteroides ovatus"
                     /db_xref="ATCC:8483"
                     /db_xref="taxon:411476"
     gene            1..2871
                     /locus_tag="BACOVA_02051"
     CDS             1..2871
                     /locus_tag="BACOVA_02051"
                     /inference="protein motif:HMMPfam:IPR000531"
                     /inference="protein motif:HMMPfam:IPR012910"
                     /inference="protein motif:superfamily:IPR008969"
                     /inference="similar to AA sequence:INSD:AAO78058.1"
                     /note="COG: NOG06412 non supervised orthologous group;
                     Psort location: OuterMembrane, score:9.49"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="EDO12162.1"
                     /db_xref="InterPro:IPR000531"
                     /db_xref="InterPro:IPR008969"
                     /db_xref="InterPro:IPR012910"
                     /translation="MSNDAIAQKAIRGTVLDEANEPIIGASVLVKGTTNGSITGVDGTF
                     NVKANPSDVLVISYVGYATVEQQVGNQTQLVIHLREDSKVLNDVVVIGYGSTTKKELTG
                     SVTSMKKDDLNPGTFTNAMGMLQGKVAGLQIINPNGADPTAKYEVLLRGTNTLSAGQGP
                     LIIIDGVAGADIRNINFQEVESIDVLKDGSAAAIYGTRGTNGVIIVTTKRARSGKTEVS
                     YDGQLTVGVVSRRAKPLSASEYKSVIKEYRPELESYIFDSDTDWFKEITQTPFSHKHNL
                     SISGGSEKFSHHTSFNYEKSDGLQKHNSSEKLMARTNIRQSLLDKWVDLDYNLNIIHRK
                     YSPSSTSAFMQAFTHNPTEPVYDDSDPDAGGYSRIKAMEYYNPVAIINERNMESKNDNY
                     GANIRATLNILPIKGLKWENFVSYDKEQYETREYYTHYYPSLIGTNGQAYIENYQENDT
                     QYESTLNYSNIFGKHSIQALLGYTYQYTYSTSASMTNSGFDFDDNQTHNIGTGTNLTEG
                     KASMSSNKEDNTYIGFFGRFMYNYDDKYLLSASLRRDGSSRFGDNNKWGWFPAVSVGWR
                     INKEKFLSNVKWIDDLKLRAGYGVTGNQDFSNYKSLMMMTTAGKFYYNGQWINTYQPAS
                     NANPDLKWEKKAEFNVGVDMTMFDNHLSFTFDYYKRTTSNLLYDYIVPTPPYVYNTLFT
                     NVGKVTNEGVELTISGTPFNTRDFTWNTSLTVSHNKNKLVKFTNDEFTNGTYKVGWSTS
                     AACYTQRLIEGQSLGTFYGPIWLGTDTDGKDVLLGQNADGSVPEEQWEKIGCAYPDATL
                     SWSNTFRYKKFDLSFSLRASIGGEILNNYAMEYENLSSIGLRNISSNWLSQTNFTSTTY
                     KYSSKYIEDASYLKLDNVTFGYTWDFTSKMIKRLRLSLTAQNVFCITGYSGVDPEVALS
                     GLEPGMESLSYYPRTTEFTFGVNIVF"
     gene            2887..4416
                     /locus_tag="BACOVA_02052"
     CDS             2887..4416
                     /locus_tag="BACOVA_02052"
                     /inference="protein motif:HMMPfam:IPR012944"
                     /inference="similar to AA sequence:REFSEQ:ZP_01719514.1"
                     /note="COG: NOG26547 non supervised orthologous group;
                     Psort location: OuterMembrane, score:9.52"
                     /codon_start=1
                     /transl_table=11
                     /product="SusD family protein"
                     /protein_id="EDO12163.1"
                     /db_xref="InterPro:IPR012944"
                     /translation="MKKTNIILSFVICILLYGCTDLSETVYTGVAMNDFFKNEKELVAN
                     AGRAYTKLQGYNSEQSLWTLLLQASDECAVPACGGSWYSNGRYEEIQTNKIPPANKLLT
                     RGWNWIFNGIAACNEIIYETELSPIQFEGKEKIIAEMKILRAFYYYQAISCWGNVPFTT
                     DYTETGYPEQKSREYIFNYLEKEINDNIEFLDREPSDTNYGRATQAAAYCILAKMYLNA
                     EAWFGTPMYDKAEKACKDIMDIGAYSIEDSYSTNFDIKNEDSKENIFVILYDRVYTSGD
                     SNSFYLHTLTLEAASQATFNIPAAPWSGFLCQPDFFQTYAEQDLRRSQSWLYGPQVDLS
                     GKDLGFEYTPVFPEEKYYNSNGGRGTYDGARCWKWHYQTDGSLKEYTVSMDNDFAIFRY
                     ADVVLMYVEALVRQNRTSEAIQLADFKKIRTRAGLDAYTTSQLTVDELYAERGRELAWE
                     GWRHEDMIRFGKYLKKYWAHPDQSSETFRNVFPIPTDILNANPKLSQNKDY"
     gene            4476..6440
                     /locus_tag="BACOVA_02053"
     CDS             4476..6440
                     /locus_tag="BACOVA_02053"
                     /inference="protein motif:HMMPfam:IPR008009"
                     /inference="similar to AA sequence:REFSEQ:ZP_01122507.1"
                     /note="COG: NOG08360 non supervised orthologous group"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EDO12164.1"
                     /db_xref="InterPro:IPR008009"
                     /translation="MKKPIIFLLVLITNILFISAQKISKTELKDKIAGAWIGQMVGNIY
                     GLPFENKFVDEPAPESRFPFGYTKNIDKLQKYNGAFSDDDTDVEYIYLLLMEKYGVEPT
                     YANMREGWMYHIRDRVWLANRAALGLMHLGFTPPFTGDENLNPHWYQIDPQLINEIWAY
                     TAPGMISYAAGKSDWAARITSDSWAVSPTVLYGAMYADAFFCKDIRKLITRALKELPAD
                     DRYAIAVKEMIALYDKYPKDWVKARQIMAKKYYIDEPAMTKTIWNANLNGLCGILAMLY
                     GEGDFQRTLDLSCAMGFDCDNQAATISGLLGVMYGAKSLPESLTKPIEGWEKPFNDRYI
                     NITRFDMPDASIEDMIERTYNKAIELVCAKGGKVKGDMVYVNPKAQFIPPMEFCIGPNP
                     DLEIGQPTDYSFACRTNADFKWELVKGKLPVGVTFQNGKLAGTPTEAGKYPITLQLSAN
                     NKNSITKDFELLVKTKNIAPKADTIYANIRKLNEEVLDSCWITFGKPMYATNVEVINDG
                     VKDGVGSVFYSLAAKSNLPKIDYFGYGWKEEHNINMLVLDMGCLEEFGGWFTSLNIQYL
                     GNDGHWYDVGKFKSTPALPETDIVFFQPPFAQYVLEFPAVETKGIRVLMDAKVQEHWHK
                     YTKNVSSFISITELGVYEK"
     gene            6453..8060
                     /locus_tag="BACOVA_02054"
     CDS             6453..8060
                     /locus_tag="BACOVA_02054"
                     /inference="protein motif:HMMPfam:IPR005502"
                     /inference="similar to AA sequence:INSD:EDJ82860.1"
                     /note="KEGG: mst:Msp_0683 0.0010 hypothetical protein
                     K00788; COG: NOG08360 non supervised orthologous group"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EDO12165.1"
                     /db_xref="InterPro:IPR005502"
                     /translation="MRKIITICIIGLFALNVQAQPVKTLKLSDKELLDKIKGGWAGQTI
                     GVVFGAPTEFKFTGTYIQDYQPIPWAEGYVKYWWEKKPGLFDDIYNDCTFVEAFDELGL
                     DCSQEELAKRFAFADYHLAHANQAGRYNIRQGIMPPASGHWLNNPHADDLDFQIEADFI
                     GLMAPAMLPEALDIASRVGHIMNSGDGFYGGAFVAALYSSAFYEKSPEAILKTAISAIP
                     EKSTFHQCIQDVINFHSLHPDNWKDCWYFLQEKWNCDVGCPKGVFLNFNIDAKLNSAFV
                     ALAMLYGKGDFTNSIDIATRCGQDSDCNPSTVGGVLGVMYGYDKIPSFWLNPLKEVEDF
                     TFEGTNMSLAKAYQMSFDQAKQLIVKTGGKVSGGEIEIPIKKADVLPLEQNFENTYPLY
                     RERKDCFLTDTFEFDFNGNGFVIWGNICCTRSITPDYINRVSTRHIGSEVFGLAEPNDP
                     YVAKVEIWIDGELDHVAALPMRNTDRKVEPAWKYLMKEGRHHVKMKWLNRKKDYIIRIN
                     DIMYYSEKKEHDRFYFNK"
     gene            8067..9614
                     /locus_tag="BACOVA_02055"
     CDS             8067..9614
                     /locus_tag="BACOVA_02055"
                     /inference="similar to AA sequence:INSD:EDJ82860.1"
                     /note="COG: NOG08360 non supervised orthologous group"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EDO12166.1"
                     /translation="MKKVLINIILLFTFSISGMAQIEYGKTVEISKEVLLDKIKGGWAG
                     QTIGCTYGGPTEFKYRGAIIHEKTPIIWYDDYCKDIFAEDPGLYDDVYMDLTFLEVMQK
                     EGINAPAESFAKAFANADYKLWHANQAARYNILHGIMPPASGHWKNNPHADDIDFQIEA
                     DFIGMICPGMVNTASNFSDKIGHIMNYGDGWYGGVYMGAMYALAYVNNDIYTIVTEALK
                     TIPEQSKFHRCITDVIKYWKQYPDDWRKCWLEIENRHAFEIGCPEGVFNAFNIDATINA
                     AYCVMGLLYGNGDFFKTMDIATRCGQDSDCNPATAAGILGVIQGYKAIPEYWKPALERC
                     ENIKFPYTDISLSSVYDINLKLLSDVLKANGGKIKGDKYYTTIQKPKTVAWEVSFEGLY
                     PSERRVIKYDLGTEKTFEFNGKGVVLMGMIRQDVKDSNDNYIAILEAYIDGKKVETIEM
                     PYDYIKRKYDIFYNYDLEEGPHKLVIKWINPNEKYAVQCKDMVVYSSTPAEPINPYK"
     gene            9627..11225
                     /locus_tag="BACOVA_02056"
     CDS             9627..11225
                     /locus_tag="BACOVA_02056"
                     /inference="protein motif:HMMPfam:IPR000801"
                     /inference="similar to AA sequence:REFSEQ:ZP_01104988.1"
                     /note="KEGG: pgi:PG0425 4.7e-23 esterase, putative K03929;
                     COG: COG0627 Predicted esterase; Psort location:
                     OuterMembrane, score:9.52"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1"
                     /protein_id="EDO12167.1"
                     /db_xref="InterPro:IPR000801"
                     /translation="MNRKLLLLLALLLFSYGLSSCSSDDNNPSEGEQTDTPELFTKRYN
                     PDQSFYSKILGQEIKYSVLLPQEYLSESTGKYGVVFLLHGWGGNQSSWGPSGLNIQSIA
                     DAQTSNGSIRPLIYIMPEGFNTYFCNRYDGKFNYMDMFINELVPLIDKRFRTTASKTER
                     AVAGFSMGGFGALSIASQHPETFSVSIGLSPSLNTDEQYISLSQDGWNLQWGNNFGGSG
                     QTGTGRLTSYYKSQCPLHFFKDKPSSTFQTVRYYIDCGDDEERLYAGNGELHSLLRDKN
                     IKHEYRVRNGAHTDSYWRESMKEALPFIERSFKGENYPQETLKKFTEELHATNKNIKVG
                     NSNIELWLPDDYNSELTYKVLYYSKGEGNVDLTTKKVAVALDSLMQIKRMIIAGFNVKE
                     MILNETNFSAITDAVEKTVHTESNADFRLGLTYGSEADYLYNQSTGNAPAINFFFAEDA
                     DIINLSAENRAKIYYLDITDEGSNYNSIFTLFNGLRGAEAPVQYRVRNGLDSEQSAQTG
                     IYSMSYYIGEQLIKK"
     gene            11227..12747
                     /locus_tag="BACOVA_02057"
     CDS             11227..12747
                     /locus_tag="BACOVA_02057"
                     /inference="protein motif:HMMPfam:IPR000801"
                     /inference="similar to AA sequence:REFSEQ:ZP_01104988.1"
                     /note="KEGG: pgi:PG0425 4.9e-28 esterase, putative K03929;
                     COG: COG0627 Predicted esterase"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1"
                     /protein_id="EDO12168.1"
                     /db_xref="InterPro:IPR000801"
                     /translation="MKKILLYIILFLSGIDGAWALPIEKEGMSIYSPSLKQEVSYSIIL
                     PEGYEHSETEYPVLYMFHGIGGDYTSWLEYGNVARVMDKMIKEGKIQPFIMVIPDGYLS
                     YYSDTYDGSSLYETFFIKELVPYIDNNYRTRKDINGRSIIGFSMGGFGALSVSLRNRHL
                     FGSVVALSPSIRTEKQYIEEGPQKGWDNQWGRIFGGVGKNGKQRLTSYYKQHSPYHILS
                     TLRNSDLKGFGIMLDIGDKEGTLCESNEELHRLLLERQIPHEWEVRSGGHDFTCWNTAL
                     PKAFRFINEYFNGKRSGNSESSLPNETPFIQTANATVYYPEQAQGSTRKYPIIYVQGEI
                     NEQQQKVLVSQFHQMVDENKTWPAVLCFVKANTDLSETISDIEKQLSGIRGSQRMRALI
                     TLGDNIKEGIEAIQGENLFTGIVCVNAIGNENDAQNLIKAVNSYKRYPRCWIEILPESK
                     EYGFSSNIHILLKESDLEHEFRSRKCKEANVFTYWEDWILYLNNRIHV"
     gene            12817..13773
                     /locus_tag="BACOVA_02058"
     CDS             12817..13773
                     /locus_tag="BACOVA_02058"
                     /inference="protein motif:HMMPfam:IPR010651"
                     /inference="similar to AA sequence:REFSEQ:NP_811721.1"
                     /note="COG: NOG04879 non supervised orthologous group;
                     Psort location: CytoplasmicMembrane, score:10.00"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid elongase 3-ketoacyl-CoA synthase 1"
                     /protein_id="EDO12169.1"
                     /db_xref="InterPro:IPR010651"
                     /translation="MICWGSWGNTQKLVSKNWRYELFYWDYVIGMVLFTILLGFTMGSH
                     GDTGRSFLEDLGQASGDSIGWVILGGVIFNASNILLSASISLAGMSVAFPLGVGIALVL
                     GVIVNYLGIPTGNPLLLFGGVALIVIAIICNGVASGKMQKGEESRKNNKKGIIIALIAG
                     VLMSLFYRFVVKGMDVENFNSPAIGMMTPYSAIFVFSIGVLLSNFVFNTLVMKCPFVGE
                     KVSYSEYFKGNTKTHICGMLGGAIWGLGSAFSYIASGEAGPAVSYALGQGAPLIAAIWG
                     VFIWKEFKGSTKSVNQLLALMFIFFLVGLGMIVVAGN"
     gene            13797..14774
                     /locus_tag="BACOVA_02059"
     CDS             13797..14774
                     /locus_tag="BACOVA_02059"
                     /inference="protein motif:Gene3D:IPR008957"
                     /inference="protein motif:HMMPfam:IPR003961"
                     /inference="protein motif:HMMSmart:IPR003961"
                     /inference="protein motif:superfamily:IPR008957"
                     /inference="protein motif:superfamily:IPR011050"
                     /note="KEGG bcl:ABC0646 0.0016 chitinase K01183"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain protein"
                     /protein_id="EDO12170.1"
                     /db_xref="InterPro:IPR003961"
                     /db_xref="InterPro:IPR008957"
                     /db_xref="InterPro:IPR011050"
                     /translation="MKKYFYLLAMLAVVLGTTACSDDDNHPSTNILDKPEVTVPDVKES
                     SAVITWKAIGNATAYIYSLNNGSEQSTDQNTIQLTGLEPEKSYTFKVKAQKTGSIYFED
                     SDYAEITFTTTSEVTVYRIATFADDWDKWYYEYNDNGTVKRVYRLYEGELDREWLFAYD
                     GNNITVTGKNAYNMTLNDQGYVATFVDGSNTYEYTYDENGYMTKVEKNGNIASNITIEN
                     GNIMQWTRFSDGVEQFKVQTYSAVPNVSGAHCIYAESSGASRWLVETGLFGKASANCHT
                     SSGWQHSSSTATYTFEYDENSCIKEESKNYDGYIENFYYTYFSE"
ORIGIN
        1 atgagcaatg atgctattgc ccagaaggca ataagaggta cagtcctcga tgaagcaaat
       61 gaacccatta tcggtgccag tgtccttgtt aaaggtacaa caaatggttc tattacagga
      121 gttgatggaa ccttcaatgt aaaagcgaat ccttctgatg tattggttat atcttatgta
      181 ggttatgcaa ctgttgaaca acaagtagga aatcagacac agcttgtaat acacctgcgc
      241 gaagactcga aagtgcttaa tgacgttgta gtaatcggct acggctctac cacaaagaaa
      301 gagcttacag gatcggttac cagcatgaag aaagatgacc tcaatcccgg aacattcacc
      361 aatgctatgg gaatgttgca aggaaaagtg gcaggattac aaattatcaa cccgaacggt
      421 gctgatccga cagccaaata tgaagtatta cttcgcggaa caaacacact ttccgcaggc
      481 caaggtccat taattattat tgatggggtg gcaggtgcag atatcagaaa tatcaatttt
      541 caggaagtgg aatcaattga cgtattaaaa gatggttcgg cagctgctat ttatggaaca
      601 agaggtacga atggagtaat catcgtcaca actaaacgtg caagaagtgg caaaacggaa
      661 gtttcttatg atgggcaact caccgtggga gtcgtttcac gcagagcaaa acctttatcg
      721 gcaagcgaat acaaatctgt tatcaaggaa tatcgcccag aattggaaag ttatattttt
      781 gacagtgata ccgattggtt taaagaaatt acacaaactc cgttcagcca taagcacaac
      841 ttgtctatat ccggaggttc cgagaagttc tcacaccaca cttctttcaa ttatgaaaag
      901 agcgatggct tgcaaaagca taatagctcc gaaaagctaa tggcacgtac caatattcgc
      961 cagtcattat tagataaatg ggtcgatttg gattataacc tgaatatcat tcatcgcaaa
     1021 tacagccctt cctctacaag tgcttttatg caggcattta cccataatcc aacagagcct
     1081 gtttatgacg acagcgatcc ggatgccgga ggatatagcc gtatcaaagc aatggaatat
     1141 tacaatccgg tagcgatcat taacgaaagg aacatggaaa gtaagaatga taattatggt
     1201 gcaaatattc gtgctactct aaacatattg cctataaaag gattaaaatg ggagaacttt
     1261 gtttcatacg acaaggaaca atatgagaca cgtgaatact acacacatta ttatccaagt
     1321 ttaataggaa caaacgggca agcctatatt gaaaactatc aggaaaacga tacacaatat
     1381 gagtctactt taaattactc gaatattttc ggaaaacatt ccattcaggc attactcgga
     1441 tatacatacc agtatactta cagcacatct gcatccatga caaattccgg tttcgacttc
     1501 gatgacaacc agactcataa catcggaaca ggaacgaatc taactgaagg caaagccagc
     1561 atgtcgtcca ataaagaaga taatacatat atcggattct tcggacgttt tatgtacaac
     1621 tacgatgata aatatctttt gtctgcttca ctacgtcgtg atggctccag ccgtttcggt
     1681 gacaataata agtggggatg gttcccggct gtcagcgtag gttggcgtat aaataaagag
     1741 aaattcctct cgaatgtcaa atggatcgat gatttaaaat tgagagccgg atacggagtt
     1801 accggtaacc aggatttctc aaactacaaa tctttgatga tgatgactac agccggtaaa
     1861 ttctattaca atggccaatg gatcaatact tatcaacctg caagtaatgc aaatccggat
     1921 ctaaaatggg agaaaaaagc agagttcaac gttggtgtcg atatgacaat gtttgacaac
     1981 cacctctcgt tcacttttga ttattacaaa cggactacat caaacttgct atatgactac
     2041 atcgtgccaa cacctcctta tgtatataat accttgttta cgaatgttgg aaaagttact
     2101 aatgaaggag ttgaattaac aatatccggt acacctttta acactaggga ctttacatgg
     2161 aatacctcac ttaccgtttc gcacaataaa aacaaattgg tgaaattcac caatgatgaa
     2221 ttcactaacg gaacatacaa agtgggatgg tctacaagtg ctgcctgcta cactcaacgt
     2281 ctgatagaag gacagtcatt gggtactttc tatggtccta tatggttagg aactgataca
     2341 gatggaaaag atgtattgct aggacaaaat gcagacggtt ccgtaccgga agaacaatgg
     2401 gaaaaaattg gctgtgctta tccggatgcc actcttagct ggagtaatac cttcagatat
     2461 aagaaattcg acttgagttt ctcactgcgt gcatctatcg gcggagaaat tctgaacaac
     2521 tatgcgatgg aatatgaaaa tctaagcagc atcggactta gaaatatctc atcaaactgg
     2581 ttatctcaaa cgaatttcac aagtacgacc tataaatatt cctccaaata tatagaggat
     2641 gcttcttatt taaaactgga taatgtaacc tttggatata cttgggattt cacatccaaa
     2701 atgataaaaa gactaagatt gtctcttact gcacagaatg tattctgtat caccggttat
     2761 tctggtgtcg acccagaagt tgctctttcc ggactggaac ccggtatgga aagtttgagc
     2821 tattatccgc gtacaacaga atttactttt ggagttaata tagtattcta aaaaaataag
     2881 attaccatga aaaagacaaa tataatatta agtttcgtta tctgtatatt attatacgga
     2941 tgtaccgatc ttagcgaaac tgtatacaca ggagtagcga tgaatgactt ctttaaaaat
     3001 gagaaagaac tggtagccaa tgcaggtaga gcgtatacga aattacaagg atacaattcc
     3061 gaacaaagtt tatggacatt gcttttacaa gcttctgatg aatgtgccgt tcccgcttgt
     3121 ggtggttcct ggtacagcaa tggacgttat gaagaaatcc agacgaataa aattccacct
     3181 gcaaataaat tattgacaag gggatggaac tggatattta atggtattgc tgcatgtaat
     3241 gaaattattt acgaaacgga attatcacct atccagtttg aaggaaaaga gaagattata
     3301 gctgaaatga aaatattgcg tgctttctac tattatcaag ccatttcttg ttggggaaac
     3361 gtacctttca caactgacta cacggaaacc ggatacccgg aacaaaaaag tcgtgaatat
     3421 attttcaact atctggaaaa agaaattaat gacaatatcg agttcctaga cagggagcct
     3481 tcagatacta attatggacg ggccacacaa gctgccgctt attgcattct tgcgaaaatg
     3541 tatctgaatg cagaagcatg gttcggtact ccaatgtatg acaaagcaga gaaagcctgt
     3601 aaagatatta tggatatagg tgcttacagc attgaagact cttattctac taattttgat
     3661 ataaaaaatg aagattctaa ggagaatatc tttgtaatcc tgtatgacag agtatatact
     3721 agcggtgaca gtaactcatt ttatttgcat acgctgacat tggaagctgc atcacaagca
     3781 acatttaata ttccggcagc cccatggtcg ggttttctct gccaaccgga ctttttccaa
     3841 acatacgcag aacaagatct tcgcagaagt cagagctggt tatacggtcc tcaagtagac
     3901 ctaagcggaa aggatcttgg tttcgaatat actcctgtct tccccgaaga aaaatattac
     3961 aatagcaacg gaggacgtgg cacatacgac ggtgcacgct gctggaaatg gcattaccag
     4021 actgatggtt ctttaaaaga atatacagtt agtatggaca acgactttgc catcttccgt
     4081 tatgccgatg ttgtattaat gtatgtagag gctttggtac gacaaaacag aacaagtgag
     4141 gccatccaac tggcagactt caagaagatt cgtacaagag cagggcttga tgcatatact
     4201 acaagccaat tgacggttga tgaactttat gcagaacgag gcagagaact ggcatgggaa
     4261 ggatggcgcc atgaagatat gatacgtttc ggaaaatatc tgaaaaaata ttgggcacac
     4321 cctgaccaaa gttcagaaac ttttagaaat gtatttccaa ttcctacgga tattttaaat
     4381 gcaaatccaa aattatctca aaacaaagat tattaaccac aattggaagg ttcacataga
     4441 aaagtgtacc ttccaacaaa tcaaccctga taagtatgaa aaaaccaatc atctttttac
     4501 ttgttttaat taccaacatc ttatttatct ctgcacagaa aatttcaaag acagagttaa
     4561 aagacaaaat agctggagct tggatcgggc aaatggtagg aaatatatat ggacttcctt
     4621 ttgagaacaa gtttgtagat gaacctgccc cggaatccag atttccattc ggttacacta
     4681 aaaacataga taaattgcag aagtataacg gtgcattctc tgatgacgat actgatgttg
     4741 agtatatcta cttactatta atggagaaat atggtgtaga acctacttat gccaatatgc
     4801 gcgaaggatg gatgtatcat atccgtgacc gtgtatggct agccaatagg gctgcactcg
     4861 gattaatgca tcttggattc acccctccgt tcacaggtga cgaaaatctg aatccgcact
     4921 ggtatcagat cgaccctcaa ttgattaacg agatatgggc gtatacagca ccgggaatga
     4981 tttcatatgc tgcaggaaaa agtgattggg cggcccgtat cacaagcgat tcgtgggcgg
     5041 tatctcccac tgtcctatat ggtgcaatgt acgctgacgc ttttttctgc aaggatatcc
     5101 gcaaactgat tacacgagcc ttgaaagaac tacctgctga tgacagatat gcgatagctg
     5161 taaaagagat gattgcttta tatgacaaat atcctaaaga ttgggtaaaa gcacgtcaaa
     5221 tcatggctaa aaaatattac atagatgaac cagccatgac aaaaacaatc tggaatgcta
     5281 atctgaacgg attatgcggg attttggcta tgttgtatgg cgaaggagat ttccaacgga
     5341 cattggattt aagctgtgca atgggatttg actgcgacaa tcaggcagcc actatctccg
     5401 gacttctagg agtaatgtat ggtgcaaagt ctcttcccga aagtttgact aaacctatcg
     5461 aaggatggga aaaacccttc aacgaccggt atatcaatat caccaggttt gatatgcccg
     5521 atgcttccat tgaagacatg attgaacgta cttacaacaa agcaatcgaa ctggtttgtg
     5581 ccaaaggagg aaaagtaaag ggtgatatgg tatatgttaa ccccaaagca caattcatcc
     5641 cccctatgga attctgtatc ggtccgaatc cggacttgga aatcggacag cctaccgatt
     5701 actcatttgc ctgcagaaca aacgctgatt tcaaatggga actggtaaaa ggtaaacttc
     5761 cggtaggagt tacattccaa aacggtaaac tggcaggaac tcctacagaa gccggcaaat
     5821 atccaattac ccttcaatta agcgcaaata acaagaactc aattactaaa gactttgagt
     5881 tattggttaa aaccaagaat atagccccca aggctgacac tatttacgca aatattcgca
     5941 aactaaacga agaagtactc gattcttgct ggattacctt tggaaaaccg atgtacgcaa
     6001 caaatgtaga agtaattaat gacggagtca aagatggcgt aggatctgta ttttattcat
     6061 tagcggccaa aagtaatctg cctaaaatag actatttcgg atacggttgg aaagaagagc
     6121 ataacatcaa tatgcttgtt ttagatatgg gctgtctgga agagttcgga ggatggttta
     6181 cttctctaaa cattcaatat cttggaaatg acggacattg gtatgacgta ggtaaattca
     6241 aatcaactcc ggcacttccc gaaaccgata ttgtattttt ccaacctccg tttgcacaat
     6301 atgttttgga attcccggca gtagaaacca agggaatacg cgtactaatg gatgcaaaag
     6361 tgcaggaaca ctggcataaa tacacaaaga atgtatcatc attcatctct atcaccgagt
     6421 tgggagttta tgagaaataa aggaggagaa gaatgaggaa aataattacg atatgtatta
     6481 tagggctttt tgctctcaac gtacaagccc agcctgtaaa aactttaaag ttatctgata
     6541 aagaactttt agataagatt aaaggaggat gggccggaca aaccattggt gttgttttcg
     6601 gagcacctac agagtttaag ttcacgggaa cttatattca agattaccag ccaatcccct
     6661 gggcggaagg ttacgtaaaa tattggtggg aaaagaagcc cggattgttc gatgatattt
     6721 ataacgattg cacgtttgtt gaggcatttg atgaacttgg cctggattgt tcacaagaag
     6781 aacttgccaa aagatttgca ttcgctgatt atcatttggc ccatgctaac caagcaggca
     6841 gatataatat ccgccaaggt atcatgccac cggcttccgg tcattggttg aataacccac
     6901 atgcagacga tctggatttc cagatcgaag cagacttcat tggtctgatg gctccggcaa
     6961 tgctgccgga agcattggat atcgcctcca gagtaggcca cattatgaat agcggtgacg
     7021 gtttttatgg cggtgctttc gtagcagctc tttatagctc cgctttctat gaaaaaagtc
     7081 ctgaagcgat tctaaaaacc gcaatctctg ccatacctga aaagagtaca ttccatcaat
     7141 gtattcagga tgtaatcaat ttccattctt tgcacccgga caactggaaa gattgctggt
     7201 acttcctgca agaaaaatgg aactgcgatg taggatgtcc caaaggcgta tttctaaact
     7261 ttaatattga cgccaagctc aattccgctt tcgtagcatt agctatgctc tacggtaaag
     7321 gagacttcac caactctatc gatattgcaa cacgctgcgg acaagattct gactgtaatc
     7381 cgtctacagt cggcggtgta ctcggcgtca tgtatgggta tgataaaatc ccttcattct
     7441 ggttaaaccc tttgaaagaa gtagaagact ttacatttga aggaacgaat atgtcgttgg
     7501 caaaagcata ccaaatgagt ttcgatcaag ctaaacaact catagtcaaa acaggtggaa
     7561 aagtatctgg tggggagata gaaattccaa tcaaaaaggc ggatgtactt cctttagaac
     7621 aaaatttcga aaacacctat ccactttata gagaacgcaa agactgcttc ctgacagata
     7681 catttgaatt tgatttcaat ggtaacggat ttgttatctg gggcaacatt tgctgcacaa
     7741 gaagtattac tcccgattat atcaatcgtg tatctacacg ccacatcggt tccgaggtgt
     7801 tcggattggc agagcccaat gacccttacg ttgctaaagt ggaaatatgg attgacggag
     7861 aactggacca tgtagcagct ttacccatga gaaacacaga ccgcaaagta gaaccagcct
     7921 ggaaatacct catgaaagaa ggaagacacc acgttaaaat gaaatggttg aaccgcaaaa
     7981 aagactacat tattcgtatc aacgacataa tgtattattc agagaaaaaa gaacatgata
     8041 gattttactt taataaataa gttaagatga aaaaagtcct gattaacatc attctactat
     8101 ttaccttttc catatcggga atggcacaaa tagaatatgg aaaaacagtt gaaataagta
     8161 aagaggtact gctcgacaaa attaaaggag gctgggccgg acaaaccata ggatgcacct
     8221 atggcggtcc tacagaattt aaatacagag gagcgatcat tcatgagaaa actccaataa
     8281 tctggtatga tgactattgc aaggacatct ttgcagaaga tccgggattg tatgatgatg
     8341 tatatatgga tcttaccttt ctggaagtaa tgcaaaaaga agggataaat gcccctgccg
     8401 aatcatttgc caaagccttt gcaaacgcag attacaaatt atggcatgcc aaccaggcag
     8461 ccagatataa catattgcac ggtattatgc caccagcatc gggacattgg aaaaataatc
     8521 cgcacgctga cgatattgat ttccagattg aagcagactt cattggaatg atatgtccgg
     8581 ggatggtaaa taccgcctcc aatttttcgg acaagatagg gcatatcatg aactacggcg
     8641 acggatggta tggaggtgta tacatgggag caatgtatgc attggcttac gtaaataacg
     8701 acatttatac aattgtcacg gaagctctta aaacaatacc cgaacaaagt aaatttcacc
     8761 gttgcatcac agacgttatt aaatattgga aacaatatcc cgatgactgg cgtaaatgct
     8821 ggctggaaat agaaaaccgg catgcttttg agattggctg cccggaaggg gtattcaatg
     8881 catttaacat tgacgccact atcaatgcgg cttactgtgt gatgggactt ttatatggca
     8941 acggcgactt tttcaaaaca atggatatag ccactcgttg cggacaagat tccgactgta
     9001 acccggcaac tgccgcagga attcttggag tcatacaagg ctacaaggct atccccgaat
     9061 actggaaacc ggcactcgaa agatgtgaaa atattaagtt cccttacaca gatatctctt
     9121 taagttctgt atacgacatt aatctcaaac tattgagtga tgtattaaaa gcaaacggag
     9181 gaaaaataaa aggagataaa tactatacca cgattcagaa gccaaaaacc gtagcatggg
     9241 aagtttcttt tgaaggctta tatccttctg aaagaagagt tatcaaatat gatctcggta
     9301 cagaaaaaac atttgagttc aatggcaagg gagttgtttt aatggggatg atcaggcaag
     9361 atgttaaaga tagtaatgac aattacatcg ccatcctgga ggcctacatt gacggcaaga
     9421 aagtagaaac catagaaatg ccttatgact acataaaaag aaagtatgac attttctata
     9481 attatgattt agaggaagga cctcacaaac tggttattaa atggattaat ccgaatgaaa
     9541 aatatgccgt tcaatgtaaa gacatggtgg tttattcatc cacaccggca gagccaatca
     9601 atccttataa ataaaagaga gaagccatga atagaaaact actcctatta ttagccttat
     9661 tattattttc ctacggattg tcatcatgtt cgtctgatga taacaatccg tccgaaggcg
     9721 aacagaccga tacaccggaa ctgtttacca aacgctataa tcccgatcaa agtttttaca
     9781 gcaaaatatt aggacaagaa atcaaatatt ccgtattact gcctcaagag tatctatcgg
     9841 aaagtacggg taaatatgga gtagttttcc ttctacatgg ctggggagga aaccaaagtt
     9901 catggggacc ttcaggtctg aatattcaaa gcatagctga tgcgcaaacc agcaatggta
     9961 gtattcgccc attgatctac atcatgccgg aaggtttcaa cacttatttt tgcaaccgct
    10021 atgacggaaa gttcaactat atggatatgt tcattaatga actcgtaccc ctgattgata
    10081 agcggttccg aacaacagcc agtaagacgg aaagagctgt tgccggattc tctatgggag
    10141 gtttcggggc attaagtata gcatcgcaac atcctgaaac attctcggta tcaataggat
    10201 taagtccctc tctaaataca gatgaacaat acatctcttt gtcacaagac ggttggaatc
    10261 ttcaatgggg aaacaacttc ggaggaagcg gccagacagg aacaggcagg ctgacaagct
    10321 attataaaag ccaatgtccg ctacactttt tcaaggataa accaagttcc acatttcaaa
    10381 cagtacggta ttacatagac tgcggtgatg atgaagaaag actatatgca ggaaacggtg
    10441 aattacattc tctattacgg gacaaaaata tcaagcacga atatcgtgta cgaaacggtg
    10501 cccatactga cagttattgg cgtgaatcca tgaaagaggc actgcctttt atcgaacgtt
    10561 cattcaaagg agaaaactat ccgcaggaaa ctctgaaaaa attcacagag gaattacatg
    10621 ctactaataa aaacataaaa gtaggtaatt ccaatatcga actatggtta ccggatgact
    10681 acaattccga actaacttac aaagttcttt attacagcaa aggagaaggt aatgttgatc
    10741 ttacaacaaa gaaggtagca gtggcattag attctcttat gcaaatcaaa cgtatgataa
    10801 ttgcaggttt taatgtgaaa gaaatgattc taaatgaaac aaatttttca gctattaccg
    10861 atgctgtgga aaagacggtt catacggaaa gcaatgcaga tttcagatta ggattaacat
    10921 acggttcaga agctgattac ttatacaatc agtcaacagg caatgcccct gctatcaact
    10981 ttttctttgc agaagacgca gatattatca acctttctgc cgagaatcgt gccaaaatct
    11041 attatttgga cataacagac gaaggcagca actacaatag catctttact ctgttcaatg
    11101 gattgcgagg tgcagaagct ccggtgcaat atcgtgtcag aaacgggctg gatagtgaac
    11161 agtccgccca aacaggtatt tattcaatga gttattatat tggtgaacag ttaataaaga
    11221 aataatatga agaagatact gttatatatt atcctatttc tatcaggtat agatggagct
    11281 tgggcacttc ccatagaaaa agaagggatg agtatttaca gtccgtccct aaagcaagaa
    11341 gtctcctact ccattatctt accggaaggt tatgaacatt cagaaacaga atatccagtc
    11401 ttatacatgt ttcatggaat aggaggtgat tataccagtt ggctcgaata cggcaacgtt
    11461 gcccgtgtaa tggataaaat gataaaagaa ggaaagattc aacctttcat tatggttatt
    11521 cctgacggtt acctgtctta ttacagtgac acttatgatg gcagttccct ttatgaaact
    11581 ttctttatca aagaactggt tccatatata gataacaact atcgtactcg caaggatatt
    11641 aatggacgct cgatcattgg tttctctatg ggaggattcg gagcattatc cgttagtttg
    11701 cgcaaccgcc acttattcgg ctctgtcgtt gcgttatcgc catccatccg tacagaaaaa
    11761 caatacatag aagaagggcc acaaaaagga tgggacaatc aatggggacg gattttcgga
    11821 ggagtgggaa agaatggtaa gcagcgccta accagttact ataaacaaca ttcaccctac
    11881 cacattcttt ccaccctccg caattccgat ttaaaaggat ttggcatcat gctggatatt
    11941 ggagataaag aaggtactct ttgcgaaagt aatgaagaac tgcaccgatt attactggaa
    12001 agacaaatac ctcatgaatg ggaagtacgt tcaggaggac atgatttcac ctgctggaat
    12061 actgcactgc ccaaagcatt cagattcata aacgaatatt tcaatggaaa gcggtcagga
    12121 aatagtgaga gtagtcttcc gaatgagact ccgtttatcc aaacagccaa tgcaacggtc
    12181 tattatccgg aacaggctca aggaagcaca agaaaatatc ctattatcta tgtacaggga
    12241 gaaattaacg aacaacagca aaaggttctc gtcagtcagt ttcatcaaat ggtagatgag
    12301 aataaaacat ggcctgctgt tctttgtttc gtaaaagcta acacagacct gtcagaaaca
    12361 atttctgata tagaaaagca actctccggc atcagaggtt cccaacgtat gcgggcttta
    12421 atcactttgg gagataatat caaagaaggt atagaagcca ttcagggaga aaatttgttt
    12481 accggcatag tctgtgtaaa tgccatcggc aacgaaaatg atgctcaaaa tcttataaaa
    12541 gcagtcaact cttacaaaag atatcccaga tgctggatag agatactccc cgaatcaaaa
    12601 gaatatggtt ttagtagtaa tatacatatc cttttgaaag aatcggacct ggaacatgag
    12661 ttccggtcaa gaaaatgtaa agaagccaat gttttcactt attgggaaga ttggatacta
    12721 tatttaaata acagaattca tgtttaacaa ttaaatacta atagtatgtt cacagtaaac
    12781 agttatttat tggcagttat attctgcatc gtaaccatga tttgttgggg ttcctggggt
    12841 aatacccaaa aactagtttc caaaaattgg cgttatgaac ttttttattg ggattatgtg
    12901 attggaatgg tattgttcac catcttacta ggcttcacaa tgggtagtca tggagatacc
    12961 ggacgttcat tcctggaaga tctgggacaa gcatcaggag acagcattgg ttgggttatc
    13021 ttggggggcg taatatttaa tgcttcaaac attcttcttt cagcgtctat ttccttggct
    13081 ggtatgtctg ttgcttttcc tttaggagtt ggcattgcat tggtattagg ggtaatcgtc
    13141 aattatctgg gaattccaac tggtaatcct ttgcttttat ttggcggtgt agctctcatt
    13201 gttattgcta tcatttgcaa tggagtggct tccggaaaaa tgcaaaaggg agaagaaagc
    13261 aggaaaaaca acaaaaaggg aattatcatt gcattaattg ccggtgtact gatgtctttg
    13321 ttctacaggt tcgtagtgaa aggtatggac gtagaaaact tcaattctcc tgctatagga
    13381 atgatgactc cttattctgc catctttgtt ttttcaatag gcgtattact aagcaatttt
    13441 gttttcaaca cattagtaat gaaatgtcct tttgtcggag aaaaagtcag ctactccgaa
    13501 tactttaaag gaaacacgaa aactcatata tgcggaatgc ttggtggagc gatatgggga
    13561 cttggttccg cgttcagcta tatcgcatca ggagaagctg gtccggcggt atcttatgca
    13621 ctaggacaag gcgcaccact tattgccgct atttggggag tatttatctg gaaagaattt
    13681 aaaggttcaa cgaaatctgt taatcaacta ctggccttga tgtttatctt cttcctggtt
    13741 ggtttgggaa tgatagtagt tgccggaaac taattattaa ccttaaatag aacaacatga
    13801 aaaagtattt ttacttatta gccatgctgg cagtagtact aggtacgact gcttgcagtg
    13861 atgacgacaa tcatcccagt acaaacattc tggataaacc ggaagttact gttcctgatg
    13921 taaaagagag ttctgctgtt attacatgga aagccatagg aaacgcaaca gcttatattt
    13981 atagcttgaa caacggtagt gaacaaagta ctgaccaaaa tacaattcaa ctgacaggtt
    14041 tagagccgga aaaaagttat acattcaaag taaaagctca aaaaaccgga tcaatctatt
    14101 ttgaagattc agactatgca gaaatcactt ttacaacaac ctctgaagta acagtttatc
    14161 ggatagccac ttttgcggac gactgggata aatggtatta cgaatataat gacaatggta
    14221 cggttaaacg tgtctacagg ctatatgaag gagagttgga tagagaatgg ctctttgctt
    14281 atgacggaaa taatatcaca gtgacaggta agaacgcata taacatgact ttaaatgacc
    14341 aaggttatgt tgcaactttt gtagatggtt ccaacactta cgaatatact tatgatgaga
    14401 atggctacat gaccaaagtt gaaaagaatg gcaatattgc atctaacatc accatagaaa
    14461 atggaaatat tatgcaatgg accagatttt cagatggagt agagcagttt aaggttcaga
    14521 cctattctgc cgtgcctaat gtgagcggag cacattgcat ctatgcggaa agcagtggag
    14581 ctagtcgttg gctggttgaa actggtctct ttggaaaagc ttcggctaat tgccatacga
    14641 gcagtggttg gcaacattca tccagtacag ctacttacac attcgaatat gacgaaaata
    14701 gttgtatcaa agaggaaagc aaaaactatg acggatatat agagaatttc tattatacat
    14761 atttctccga atag
//
