LOCUS       CR626927                4930 bp    DNA     linear   BCT 06-FEB-2015
DEFINITION  Bacteroides fragilis NCTC 9343, complete genome.
ACCESSION   CR626927
VERSION     CR626927.1
DBLINK      BioProject: PRJNA46
            BioSample: SAMEA1705957
KEYWORDS    complete genome.
SOURCE      Bacteroides fragilis NCTC 9343
  ORGANISM  Bacteroides fragilis NCTC 9343
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Cerdeno-Tarraga,A.M., Patrick,S., Crossman,L.C., Blakely,G.,
            Abratt,V., Lennard,N., Poxton,I., Duerden,B., Harris,B., Quail,M.A.,
            Barron,A., Clark,L., Corton,C., Doggett,J., Holden,M.T., Larke,N.,
            Line,A., Lord,A., Norbertczak,H., Ormond,D., Price,C.,
            Rabbinowitsch,E., Woodward,J., Barrell,B. and Parkhill,J.
  TITLE     Extensive DNA inversions in the B. fragilis genome control variable
            gene expression
  JOURNAL   Science 307 (5714), 1463-1465 (2005)
   PUBMED   15746427
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Cerdeno-Tarraga,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2004) Cerdeno-Tarraga A.M., submitted on behalf of
            the Pathogen Sequencing Unit, Sanger Institute, Wellcome Trust
            Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: amct@sanger.ac.uk
COMMENT     Accession number for plasmid pBF9343 in this genome project:
            CR626928.
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Bacteroides fragilis NCTC 9343"
                     /mol_type="genomic DNA"
                     /culture_collection="ATCC:25285"
                     /culture_collection="NCTC:9343"
                     /type_material="type strain of Bacteroides fragilis"
                     /db_xref="taxon:272559"
     gene            1..1125
                     /gene="manA"
                     /locus_tag="BF9343_0736"
     CDS             1..1125
                     /gene="manA"
                     /locus_tag="BF9343_0736"
                     /old_locus_tag="BF0771"
                     /EC_number="3.2.1.78"
                     /note="Similar to Pseudomonas fluorescens mannan
                     endo-1,4-beta-mannosidase precursor ManA SWALL:MANA_PSEFL
                     (SWALL:P49424) (419 aa) fasta scores: E(): 1.7e-30, 34.86%
                     id in 370 aa, to Cellulomonas fimi Man26A SWALL:Q9XCV5
                     (EMBL:AF126471) (1010 aa) fasta scores: E(): 5.2e-31,
                     34.09% id in 396 aa, and to Pseudomonas fluorescens mannan
                     endo-1,4-beta-mannosidase precursor ManA SWALL:CAA57670
                     (EMBL:X82179) (423 aa) fasta scores: E(): 1.7e-30, 33.65%
                     id in 410 aa. Note: BF0764, (372 aa) fasta scores: E():
                     1.6e-42, 35.401% identity in 387 aa overlap"
                     /codon_start=1
                     /transl_table=11
                     /product="GH26"
                     /protein_id="CAH06517.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_0736"
                     /db_xref="EnsemblGenomes-Tr:CAH06517"
                     /db_xref="GOA:Q5LH69"
                     /db_xref="InterPro:IPR000805"
                     /db_xref="InterPro:IPR013781"
                     /db_xref="InterPro:IPR016714"
                     /db_xref="InterPro:IPR017853"
                     /db_xref="InterPro:IPR022790"
                     /db_xref="UniProtKB/TrEMBL:Q5LH69"
                     /translation="MIKRIKILATGALLLAGLGACSPSGKKTGADSTVDTLRTAETVNL
                     LNNLRKVPTQGIMFGHHDDPLYGVGWEGDEDRSDVKSVCGDYPAVMSFDLGHIELEREK
                     SLDNVPFRKIRQETINQYKRGGVVSFSWHLDNPLTGKDAWDVSDTTVVASILPGGVHHA
                     KFISWLDAVAAFMNTLETEEGTKIPVIFRPWHEHTGSWFWWGQNLCTADQYKALWRMTH
                     DRMHARGVKNLLYAYSPGSEPKDSTAYLERYPGDDIIDLVGFDTYQFDRTQYMEQLDKS
                     LAILTEVGKAHDKPIAITETGFEAIPDSVWWTQTLYPVISKYPISYVLVWRNARERVNH
                     YYAPYPGQVSADDFVKFYREPKTLFVSDVKNLYK"
     misc_feature    1..78
                     /gene="manA"
                     /locus_tag="BF9343_0736"
                     /note="Signal peptide predicted for BF0771 by SignalP 2.0
                     HMM (Signal peptide probability 1.000) with cleavage site
                     probability 0.464 between residues 26 and 27"
     misc_feature    31..63
                     /gene="manA"
                     /locus_tag="BF9343_0736"
                     /note="PS00013 Prokaryotic membrane lipoprotein lipid
                     attachment site."
     misc_feature    100..1011
                     /gene="manA"
                     /locus_tag="BF9343_0736"
                     /note="Pfam match to entry PF02156 Glyco_hydro_26, Glycosyl
                     hydrolase family 26, score 114.4, E-value 1.9e-31"
     gene            1162..2334
                     /locus_tag="BF9343_0737"
     CDS             1162..2334
                     /locus_tag="BF9343_0737"
                     /old_locus_tag="BF0772"
                     /note="Similar to Bacteroides thetaiotaomicron conserved
                     hypothetical protein BT3028 SWALL:AAO78134 (EMBL:AE016938)
                     (396 aa) fasta scores: E(): 1.5e-122, 72.56% id in 390 aa,
                     and to Thermotoga maritima hypothetical protein Tm1225
                     SWALL:Q9X0V2 (EMBL:AE001779) (326 aa) fasta scores: E():
                     8.5e-16, 30.06% id in 326 aa, and to Methanosarcina
                     acetivorans hypothetical protein Ma2382 SWALL:Q8TNB1
                     (EMBL:AE010928) (335 aa) fasta scores: E(): 1.1e-14, 30.03%
                     id in 333 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GH130"
                     /protein_id="CAH06518.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_0737"
                     /db_xref="EnsemblGenomes-Tr:CAH06518"
                     /db_xref="GOA:Q5LH68"
                     /db_xref="InterPro:IPR007184"
                     /db_xref="InterPro:IPR023296"
                     /db_xref="InterPro:IPR028583"
                     /db_xref="PDB:3WAS"
                     /db_xref="PDB:3WAT"
                     /db_xref="PDB:3WAU"
                     /db_xref="PDB:4KMI"
                     /translation="MSLFNDKVAKLLAGHEALLMRKNEPVEEGNGVITRYRYPVLTAAH
                     TPVFWRYDLNEETNPFLMERIGMNATLNAGAIKWDGKYLMLVRVEGADRKSFFAVAESP
                     NGIDNFRFWEYPVTLPEDVVPATNVYDMRLTAHEDGWIYGIFCAERHDDNAPIGDLSSA
                     TATAGIARTKDLKNWERLPDLKTKSQQRNVVLHPEFVDGKYALYTRPQDGFIDTGSGGG
                     IGWALIDDITHAEVGEEKIIDKRYYHTIKEVKNGEGPHPIKTPQGWLHLAHGVRNCAAG
                     LRYVLYMYMTSLDDPTRLIASPAGYFMAPVGEERIGDVSNVLFSNGWIADDDGKVFIYY
                     ASSDTRMHVATSTIERLVDYCLHTPQDGFSSSASVEILKNLIERNLRLMK"
     misc_feature    1915..2250
                     /locus_tag="BF9343_0737"
                     /note="Pfam match to entry PF04041 DUF377, Domain of
                     unknown function (DUF377), score 45.2, E-value 5.9e-13"
     gene            2351..3739
                     /locus_tag="BF9343_0738"
     CDS             2351..3739
                     /locus_tag="BF9343_0738"
                     /old_locus_tag="BF0773"
                     /note="Similar to Xanthomonas campestris cation symporter
                     YnaJ or XCC4078 SWALL:Q8P3J3 (EMBL:AE012530) (460 aa) fasta
                     scores: E(): 1.7e-42, 37.63% id in 457 aa, and to
                     Escherichia coli O6 hypothetical symporter YicJ or C4490
                     SWALL:Q8FC70 (EMBL:AE016769) (479 aa) fasta scores: E():
                     2e-40, 34.91% id in 464 aa, and to Salmonella typhi, and
                     Salmonella typhimurium sodium:galactoside family symporter
                     YicJ or t3769 or STM3750 or STY4043 SWALL:Q8XEW1
                     (EMBL:AE016846) (460 aa) fasta scores: E(): 3e-40, 33.69%
                     id in 457 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A1S5F2|2.A.2.3.6"
                     /protein_id="CAH06519.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_0738"
                     /db_xref="EnsemblGenomes-Tr:CAH06519"
                     /db_xref="GOA:Q5LH67"
                     /db_xref="InterPro:IPR001927"
                     /db_xref="InterPro:IPR016196"
                     /db_xref="InterPro:IPR020846"
                     /db_xref="UniProtKB/TrEMBL:Q5LH67"
                     /translation="MTAMITLKEKIGYGLGDMASSMFWKLFGSYLMIFYTDVFGLPAAV
                     VGTMFLITRVWDSAFDPIVGVIADRTQTRWGKFRPYLLYLAVPFALIGIFTFTTPELND
                     TGKLVYAYITYSLMMMVYSAINVPYASLLGVMSPDPKERNTLSTYRMTFAYIGSFIALL
                     LFMPMVNLFGGAEDEQRGWMLSVVVIAVMCAALFYLCFALTRERVKPIREVQNSLKDDL
                     KDLLHNRPWWILLGAGVAALVFNSIRDGATVYYFKYFVVEEDYSTVSFFGVSFVLSGLY
                     LAVGQAANIVGVILAAPVSNRIGKKNTYMGAMSLATLLSVIFYWFGKGDITLIFVFQVL
                     ISICAGSIFPLLWSMYADCADYSELKTGNRATGLIFSSSSMSQKFGWAIGSALTGWLLA
                     YFGFRANEVQSVEAIHGIKMFLSWLPAVGTVLSVVFISMYPLSEKKMREVTSELEKRRK
                     AIQS"
     misc_feature    order(2438..2506,2591..2650,2678..2746,2807..2866,
                     2894..2962,3023..3091,3167..3235,3272..3331,3341..3409,
                     3503..3562,3605..3673)
                     /locus_tag="BF9343_0738"
                     /note="11 probable transmembrane helices predicted for
                     BF0773 by TMHMM2.0 at aa 30-52, 81-100, 110-132, 153-172,
                     182-204, 225-247, 273-295, 308-327, 331-353, 385-404 and
                     419-441"
     gene            3752..4930
                     /locus_tag="BF9343_0739"
     CDS             3752..4930
                     /locus_tag="BF9343_0739"
                     /old_locus_tag="BF0774"
                     /note="Similar to Rhizobium meliloti hypothetical protein
                     ra0026 or SMA0058 SWALL:Q931B8 (EMBL:AE007197) (398 aa)
                     fasta scores: E(): 7.2e-13, 23.68% id in 380 aa, and to
                     Homo sapiens N-acylglucosamine 2-epimerase RenBP
                     SWALL:RNBP_HUMAN (SWALL:P51606) (417 aa) fasta scores: E():
                     1.4e-10, 24.44% id in 405 aa, and to Mus musculus
                     renin-binding protein RenBP SWALL:AAO66340 (EMBL:AY183138)
                     (419 aa) fasta scores: E(): 2.6e-10, 23.71% id in 409 aa,
                     and to Bacteroides thetaiotaomicron N-acylglucosamine
                     2-epimerase BT3605 SWALL:AAO78710 (EMBL:AE016941) (388 aa)
                     fasta scores: E(): 1.3e-07, 24.45% id in 409 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="conserved hypothetical protein"
                     /protein_id="CAH06520.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_0739"
                     /db_xref="EnsemblGenomes-Tr:CAH06520"
                     /db_xref="GOA:Q5LH66"
                     /db_xref="InterPro:IPR008928"
                     /db_xref="InterPro:IPR012341"
                     /db_xref="InterPro:IPR028584"
                     /translation="MDEILKQEMQKELTTRILPYWMERMVDQENGGFYGRITGQEELMP
                     RADKGAILNARILWTYSAAYRLLGREEYKEMANRAKRYLIDHFYDSEFGGVYWSLNYRG
                     EPLDTKKQIYAIGFAIYGLSEFHRATGDPEALMYAVRLFNDIESHSFDGLKNGYCEALT
                     REWNEIADMRLSEKDANERKTMNTHLHILEPYTNLYRVWKDARLERQLYNLIGLFTEKI
                     LDKDTSHLQLFFDNDWQSKYPVVSYGHDIEASWLLHEAARVLGDAGLIAEIEPVVKKIA
                     AAASEGLTSDGGMIYEKNLTTGHIDGDYHWWVQAETVVGYYNLFRYFGDRGALQHSIDC
                     WEFIKRHLTDDVHGEWFWSLRADGSLNRDDDKAGFWKCPYHNGRMCIELLGE"
ORIGIN
        1 atgataaaga gaataaagat attagctaca ggtgcactgc tattggcagg attgggtgct
       61 tgttcacctt ccggaaagaa aacaggagcg gattcgactg tcgacactct gcgaacggcg
      121 gaaacagtga atttactgaa taatctacgg aaggttccca cacaggggat tatgtttggt
      181 catcatgacg atccgcttta cggtgtcggc tgggaaggtg acgaagatcg cagtgacgtg
      241 aaaagtgtgt gtggtgatta tccggctgtc atgtcgtttg atctgggcca cattgaactg
      301 gaaagagaga aaagtctgga taacgtgccg tttcgcaaaa tacgtcagga gacgattaat
      361 caatataaaa ggggaggagt ggtttctttt agctggcatc tcgataaccc cttgaccggt
      421 aaagatgcgt gggatgtgag tgatacgacg gttgtagctt ccatactgcc cggtggtgta
      481 catcatgcga aatttataag ttggctcgat gctgttgcag cctttatgaa tactttggag
      541 acggaagaag gtacaaaaat accggttatt ttccgtcctt ggcacgagca taccggcagt
      601 tggttctggt ggggacaaaa tctttgcacg gccgaccagt ataaggctct ttggcggatg
      661 acgcatgatc gtatgcatgc ccggggagta aagaacctgc tttatgctta ttcaccggga
      721 tcggaaccca aagattcgac tgcttatctg gagcgttatc cgggagatga tatcatcgac
      781 ctggtgggct ttgacaccta tcagttcgac cggacacaat atatggagca attggataag
      841 tcgcttgcta tcctgactga agtaggtaag gcgcacgata agcctatagc cattaccgaa
      901 accggtttcg aggctattcc cgattctgtc tggtggacac agactctcta tccggtaatc
      961 agcaagtatc ctatcagtta tgtgttggtg tggcgcaatg cacgtgaaag ggtaaaccac
     1021 tattatgctc cttatcccgg acaggtgtcc gccgatgact ttgtgaagtt ctaccgtgaa
     1081 ccgaaaactc tgtttgtgtc ggacgtgaag aacctttata aatagaaaat caaataaata
     1141 acgataaaaa cactgtccat tatgagtctg tttaatgata aagttgctaa attgcttgcc
     1201 gggcatgaag cactgctgat gcgtaagaat gaaccggtag aagagggaaa cggagtgatt
     1261 acgcgttacc gttaccctgt actgactgca gcgcatactc ctgtcttctg gcgatacgac
     1321 ctgaacgagg agacgaatcc ttttttgatg gaacgtatcg gtatgaatgc gacgttgaat
     1381 gccggagcca ttaagtggga tgggaagtac ctgatgttgg tgagagtgga gggagcagac
     1441 cgcaaatctt tttttgctgt tgccgaaagc ccgaacggta ttgataattt ccgcttctgg
     1501 gagtatccgg tgaccttacc cgaagatgtg gttcctgcaa ccaatgtata cgatatgcgt
     1561 ctcactgccc atgaagatgg gtggatatat ggcatctttt gtgccgaacg gcacgatgac
     1621 aatgctccca taggtgattt atcgtcagct acagccactg ccggcattgc ccgtaccaaa
     1681 gacctgaaaa attgggaacg tctgccggat ctgaaaacaa agagtcagca acgtaatgtg
     1741 gtgctgcatc ccgagtttgt ggatggaaag tatgcacttt atacccgtcc gcaagacgga
     1801 tttatcgata ccggtagcgg aggtggtatc ggatgggcat tgattgacga tataacccac
     1861 gccgaggttg gagaagagaa gatcatcgac aaacgatatt atcataccat caaggaggtg
     1921 aagaacggtg aagggccgca tcctatcaag actcctcagg gatggcttca tctggcacac
     1981 ggagtacgca attgtgctgc cgggctcagg tatgtattgt atatgtatat gacatcgttg
     2041 gatgatccca cccggctgat agcttctccg gcggggtact ttatggctcc ggtaggagaa
     2101 gagcgcattg gggatgtgtc gaatgtgctt ttttcgaatg gttggatagc cgacgatgac
     2161 ggaaaagtat ttatctacta tgcttcgtcg gacacccgta tgcatgtagc tacctcaact
     2221 atcgaacggt tggtggatta ctgcctgcac actcctcagg acggcttttc ttcctcagct
     2281 tcggtagaga tactgaaaaa cctgattgaa cgaaatctga gattgatgaa ataagaaaac
     2341 caagaataga atgactgcca tgattacact gaaagagaag atcggttacg gactgggcga
     2401 tatggcttcg tccatgttct ggaaactgtt cgggtcctat ctgatgattt tttataccga
     2461 tgtattcggc ttgcctgctg ccgtggtagg aaccatgttt ctgattaccc gggtatggga
     2521 ttcggctttc gatccgatcg tgggagtgat tgccgatcgc acacagaccc gctgggggaa
     2581 atttcgtcct tatctactgt atcttgccgt tccttttgca ctgattggta tttttacttt
     2641 caccactccg gagttgaatg ataccggaaa actggtctat gcctacatca cctattcttt
     2701 gatgatgatg gtatattcgg ctatcaatgt gccttatgct tcactgctgg gagtgatgag
     2761 tcctgacccg aaagaacgga ataccctgtc cacttaccgt atgacttttg cctatatcgg
     2821 cagttttatt gctttgctgc tcttcatgcc gatggtcaac ctgtttggtg gtgcagaaga
     2881 cgagcaacgg ggatggatgt tgagtgtggt ggtgattgct gtgatgtgtg cggctctgtt
     2941 ttatctttgt ttcgccttga cacgtgaacg ggtaaaaccg atcagggaag tacaaaactc
     3001 cctgaaagac gatttgaaag atttgcttca caaccgtccg tggtggattc tgctcggagc
     3061 cggagtggca gctttggtat tcaattctat tcgtgacgga gctacggttt attacttcaa
     3121 gtactttgtt gtggaagagg attactccac ggtttccttc tttggcgttt cttttgtgct
     3181 gagcggcctt tatctggcag tgggacaggc tgccaatatt gtgggagtga ttcttgcagc
     3241 tccggtcagt aaccgtattg gcaaaaaaaa cacatacatg ggagccatga gtctggctac
     3301 tctcctttcc gttatctttt attggtttgg gaaaggagac atcaccctga tttttgtttt
     3361 tcaggtgctg atcagtatct gtgccggaag tattttccct ttgctctggt ccatgtacgc
     3421 cgactgtgct gactattcgg aactgaagac cggcaaccgg gctacaggtc tgatcttttc
     3481 gtcttcgtcc atgagccaga agttcggttg ggctatcgga agtgcactga ccggttggtt
     3541 gttggcttac ttcggctttc gtgccaacga agtgcagagt gtagaggcta ttcatgggat
     3601 taagatgttt ctcagctggt tgcccgctgt cggaacagtt ctgtccgtcg ttttcatcag
     3661 tatgtatccg ctatcggaga agaagatgag agaggtgact tcagagttgg agaagagaag
     3721 aaaggctatt caatcataat tcagacatca tatggacgag attcttaaac aagaaatgca
     3781 gaaagagctt actacccgta ttcttcctta ctggatggaa cggatggtag atcaggagaa
     3841 cggtggattt tacggacgca tcaccggaca ggaggaatta atgccccggg ccgataaagg
     3901 ggctattctg aatgcgcgta ttttatggac ttattctgct gcctatcgtc tgctgggtag
     3961 agaggagtac aaagagatgg caaaccgtgc caaacgatac cttatcgacc acttttatga
     4021 ttccgagttc ggaggggtct actggtcact caattataga ggtgagccgc tggataccaa
     4081 gaaacagatt tatgccatcg gctttgccat ttacggactg agcgagttcc atcgggctac
     4141 cggagatccg gaagcattga tgtatgccgt ccgtttattc aatgatatag agtcccacag
     4201 ctttgatggg ctgaagaacg gttattgtga agcgcttacc cgtgaatgga acgaaatagc
     4261 cgatatgcgc ctcagcgaga aagatgcgaa tgaacgcaag actatgaata cccatctgca
     4321 tatcctcgaa ccttacacca acctgtaccg ggtctggaaa gatgcacggc tggaacgtca
     4381 gctctacaac ctgataggac tttttacaga gaagatactg gataaggaca catcccattt
     4441 acaactcttt ttcgataacg actggcaaag caaatacccg gtcgtctctt atggacatga
     4501 tatcgaagcc tcatggttgt tgcatgaagc cgcccgggta ttgggagacg ccggactcat
     4561 tgcggagata gaacctgttg taaagaagat agctgcggct gcatccgaag gacttacttc
     4621 cgacggagga atgatatatg aaaagaatct cactaccgga cacatcgacg gcgactacca
     4681 ttggtgggta caagccgaaa ccgtagtcgg atactataac ctgttccgat atttcggtga
     4741 tcgaggggct ttgcaacatt ccatcgactg ctgggagttt attaaacgac atttgactga
     4801 cgatgtgcat ggcgaatggt tctggagcct tcgtgccgac ggtagcctga accgggatga
     4861 tgataaggcc ggcttctgga aatgtcctta tcataacgga cgtatgtgca tcgagctgtt
     4921 gggcgaataa
//
