LOCUS       EU670680                4730 bp    DNA     linear   BCT 21-MAR-2009
DEFINITION  Lactobacillus casei strain ATCC 27139 lac and gal operons, complete
            sequence.
ACCESSION   EU670680
VERSION     EU670680.1
KEYWORDS    .
SOURCE      Lactobacillus casei
  ORGANISM  Lactobacillus casei
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactobacillus.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Tsai,Y.K., Chen,H.W., Lo,T.C. and Lin,T.H.
  TITLE     Specific point mutations in Lactobacillus casei ATCC 27139 cause a
            phenotype switch from Lac- to Lac+
  JOURNAL   Microbiology (Reading, Engl.) 155 (PT 3), 751-760 (2009)
   PUBMED   19246746
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Tsai,Y.K. and Lin,T.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2008) Life Science, National Tsing Hua University,
            101, Section 2 Kuang Fu Road, Hsinchu, Taiwan 30013, Republic of
            China
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Lactobacillus casei"
                     /mol_type="genomic DNA"
                     /strain="ATCC 27139"
                     /culture_collection="ATCC:27139"
                     /db_xref="taxon:1582"
     operon          1..4730
                     /operon="lac"
     gene            1..894
                     /gene="lacT"
                     /operon="lac"
     regulatory      1..5
                     /regulatory_class="ribosome_binding_site"
                     /gene="lacT"
     CDS             16..894
                     /gene="lacT"
                     /operon="lac"
                     /note="antiterminator protein"
                     /codon_start=1
                     /transl_table=11
                     /product="LacT"
                     /protein_id="ACG55690.1"
                     /translation="MPKIAQIFNNNVALVDLDNRGQAVVRGRGIAFQKRRGDVIPTKQI
                     EKIFYLANETSRQNLYFLLKNIPIDVVTTTYEIIDVAQKQYRLKVLDYIYITLSDHIYE
                     AYKRYQAGTYQETMVPDFHIQYPAEYAVAKQALQIIATNLGVQFPQSEIKNLALHFINA
                     SGEDDGEQVFGKSNEASLSQLVQEVLKRHHITRSHSNGNYYDRFMIHLQYLIDRLQRVD
                     TYAVTIVPEVATELKQNYPQSYKIASEIFDEIKDQLYRSMSEDERLYFIIHIQRLINEA
                     PAQNHSQNDSL"
     gene            1001..2748
                     /gene="lacE"
                     /operon="lac"
     regulatory      1001..1005
                     /regulatory_class="ribosome_binding_site"
                     /gene="lacE"
     CDS             1015..2748
                     /gene="lacE"
                     /operon="lac"
                     /note="EIIBC lactose PTS"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q045H3|4.A.3.1.3"
                     /protein_id="ACG55691.1"
                     /translation="MNKVFDKLKPVFEAIAANKYISAIRDGFIACMPIIIFSSIFMMVA
                     YVPNAWGFYWPDNVTNTLMVAYKYSMGLLALFVAGTTAKNLTDSKNLELPKTNQINPVA
                     VIVASEISFVILSILPLKTGVDLTYMGTQGLICAYIVGLIVPNIYYVCIKNNVTIKLPA
                     QVPGNIAQSFKDLIPMGLSVTAFWLFGVGFKAATGTVLPRWIIQVLSPLFQASDSYLGL
                     ALIAGAMAFFWFCGVQGPSIVQPAVVPIMIANTAANLQQYQAGQHVSHVLAMNTMDYVM
                     NFGGTGSTFVVAFIMLFAARSAQLKAVVKAAFVPCTFGVNEPVLFGMPIIMNPIFFIPF
                     LATPIVNVCLFKFFVSVLGMNSMMYTMPWTVPGPIGILISTGFAPLAFVFVLLTLVLDV
                     AIYFPFIRVYDSTLLAEEKAKEEVIEDDGMAVLASDTVSPSIPTGLTVATATDDDATHV
                     LPETAPSAHGEAYFKQNEVDVLVLCAGGGTSGILANALNKLSKERGLKLSAAARAYGQD
                     MDLIKDMNMVILAPQMESMKGNLKKITDKYGVKLVTTTGRQYIELTNNGDMALDFVESN
                     L"
     gene            2760..4199
                     /gene="lacG"
                     /operon="lac"
     regulatory      2760..2764
                     /regulatory_class="ribosome_binding_site"
                     /gene="lacG"
     CDS             2775..4199
                     /gene="lacG"
                     /operon="lac"
                     /note="phospho-beta-galactosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1"
                     /protein_id="ACG55692.1"
                     /translation="MSKQLPQDFVMGGATAAYQVEGATKEDGKGRVLWDDFLDKQGRFK
                     PDPAADFYHRYDEDLALAEKYGHQVIRVSIAWSRIFPDGAGEVEPRGVAFYHKLFADCA
                     AHHIEPFVTLHHFDTPERLHEAGDWLSQEMLDDFVAYAKFCFEEFSEVKYWITINEPTS
                     MAVQQYTSGTFPPAESGRFDKTFQAEHNQMVAHARIVNLYKSMQLGGQIGIVHALQTVY
                     PYSDSAVDHHAAELQDALENRLYLDGTLAGEYHQETLALVKEILDANHQPMFQSTPQEM
                     KAIDEAAHQLDFVGVNNYFSKWLRAYHGKSETIHNGDGTKGSSVARLQGVGEEKLPDGI
                     ETTDWDWSIYPRGMYDILMRIHNDYPLVPVTYVTENGIGLKESLPENATPDTVIEDPKR
                     IDYVKKYLSAMADAIHDGANVKGYFIWSLQDQFSWTNGYSKRYGLFFVDFPTQNRYIKQ
                     SAEWFKSVSETHIIPD"
     gene            4236..4583
                     /gene="lacF"
                     /operon="lac"
     regulatory      4236..4240
                     /regulatory_class="ribosome_binding_site"
                     /gene="lacF"
     CDS             4245..4583
                     /gene="lacF"
                     /operon="lac"
                     /note="EIIA lactose PTS"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q045H4|4.A.3.1.3"
                     /protein_id="ACG55693.1"
                     /translation="MMATKEEISMVGFALVAYAGDARTAAVHALDAAEAGDFDKANELV
                     EKAQQDINEAHNQQTQLLSQEAGGAEMDVTFIMVHGQDTLMTTMLLIDETRYMIRMFKR
                     IKELENKQ"
     regulatory      4693..4730
                     /regulatory_class="terminator"
                     /operon="lac"
                     /note="rho-independent"
ORIGIN
        1 ggaggtgatg acaacttgcc aaaaatagct cagattttta acaacaacgt ggccttggtt
       61 gatctagaca accgcggcca agccgttgta aggggacgtg gcatcgcttt tcagaagagg
      121 cgaggagatg ttattccgac aaagcagata gagaagatct tttatctagc gaacgagact
      181 tcccgacaaa atttgtactt tctcttaaaa aatattccga ttgacgtggt gacgactacc
      241 tatgaaatta ttgatgttgc ccagaaacaa tatcgactga aagtacttga ttatatctac
      301 attaccttga gtgatcatat ttacgaggca tataaacgct atcaggcagg gacttatcaa
      361 gaaacaatgg taccagattt tcatattcaa tatccggccg aatatgcggt ggctaaacag
      421 gcactgcaaa tcattgccac gaaccttggc gttcagtttc cacagtcgga aataaaaaat
      481 ctggcgttgc actttatcaa cgcttccggc gaagacgatg gcgagcaggt ttttggtaaa
      541 agcaatgaag cctcacttag tcaacttgta caagaagtgt tgaagcgtca tcacattact
      601 cgctctcatt caaatggcaa ctactatgac cgatttatga ttcatctcca gtatctcatc
      661 gaccgactgc agcgtgttga tacatatgcc gttaccattg tccctgaggt tgccactgaa
      721 cttaagcaaa actatccgca gtcttacaag attgcctcag aaattttcga tgaaattaag
      781 gatcaactct atcgcagtat gagtgaggac gaacgacttt acttcatcat ccacattcag
      841 cgattgataa acgaagcacc agcccagaat cattcacaaa acgattcatt ataacgcgct
      901 cgcagtcgta gaagcctaca cataagggct ttgaagcaat ctaccaaaga ttgggccagt
      961 ttgcttcaag cacgcttatg ctttggcttc caagcgctca ggaggaaaag actcatgaat
     1021 aaggtttttg ataaattaaa accggttttt gaagccatcg ctgctaacaa atatatttcc
     1081 gcgattcgtg atggctttat cgcatgtatg ccgatcatca tcttctcaag tatctttatg
     1141 atggttgctt atgttccgaa cgcttggggg ttttactggc cagacaacgt aacgaatacg
     1201 ttgatggttg cttataaata ttccatgggc ttgctggcac tgtttgtggc gggaacgact
     1261 gctaaaaact tgactgacag caagaacctt gaattaccga aaacaaatca aattaatcca
     1321 gtggccgtca ttgtggcgtc tgaaatttct tttgtcattt tgtcgatctt accgcttaaa
     1381 accggtgttg acctgactta catggggact cagggattga tctgcgctta tatcgttggg
     1441 ttgatcgtgc caaatattta ctacgtctgt attaagaaca atgtcacgat taaattacca
     1501 gcgcaggtac caggtaatat cgcccagtca tttaaagacc ttatcccgat gggcctttct
     1561 gtgacagcct tctggctttt cggtgttggc ttcaaagcgg caacgggtac tgtattgcca
     1621 cgctggatca ttcaagtact ttcgccgctt ttccaagcca gcgattctta tctaggctta
     1681 gccctgattg ctggcgcgat ggctttcttc tggttctgtg gtgtccaagg cccatcaatt
     1741 gttcagccgg ctgttgtccc gattatgatt gcgaatacag cagctaactt gcagcaatat
     1801 caggcagggc aacatgtctc gcacgttttg gccatgaaca cgatggacta cgtcatgaat
     1861 tttggtggaa ccggatcaac atttgtggta gctttcatca tgctgtttgc ggcccgatca
     1921 gcgcaattaa aagccgttgt taaggctgcc tttgtgccat gcactttcgg ggtcaatgaa
     1981 cctgttttgt tcggaatgcc gattatcatg aatcccattt tcttcattcc gttcttagca
     2041 accccgatcg tgaacgtttg cttattcaaa ttctttgtga gtgtacttgg catgaacagt
     2101 atgatgtaca ccatgccatg gacagttccg ggaccgattg ggattttgat ttcgactggg
     2161 tttgccccgt tggctttcgt ctttgttcta ctgacgttgg ttctggacgt tgcgatatac
     2221 ttcccattca ttcgcgttta tgacagcaca ctcttagctg aagaaaaagc taaggaagag
     2281 gtcattgaag acgacggcat ggctgttctg gcgagtgaca cagtgtcacc ttctatcccg
     2341 actggtttga ctgttgcgac tgccacagac gacgatgcga cccatgtgtt gcctgaaaca
     2401 gccccatctg cccacggtga agcctatttc aaacaaaatg aagttgatgt tttagtgttg
     2461 tgtgctggtg gtggaacaag cggtattctt gccaacgcgc taaacaaact gtctaaggaa
     2521 cgcggtttaa agctctcggc cgccgcccgc gcttatggac aggatatgga tttgatcaaa
     2581 gatatgaaca tggtcatcct tgcgccgcaa atggaaagca tgaaaggtaa tttaaaaaag
     2641 atcaccgata agtatggcgt caagctggtc acaacaactg gtcggcaata cattgaatta
     2701 accaacaatg gtgacatggc acttgatttt gttgaatcca atctttgaga cacttaacag
     2761 gaggttatta agcaatgagt aaacagctac ctcaagattt tgtaatgggc ggggcaaccg
     2821 ccgcctatca agtcgaagga gccaccaagg aagacggtaa aggtcgcgtg ctttgggacg
     2881 attttctgga taaacagggg cggttcaagc ccgacccggc agcagatttt tatcatcgtt
     2941 atgatgaaga tttggcatta gcggaaaaat acggccatca agtgattcgg gtgtcaattg
     3001 cctggtcgcg aatttttccg gatggtgccg gggaagttga gccacgcggg gttgcttttt
     3061 accacaagct cttcgcggat tgtgccgccc atcatatcga accgtttgtt acgctacatc
     3121 attttgatac gcccgaacgc ttgcacgaag ccggtgattg gttgagtcaa gagatgttgg
     3181 acgactttgt ggcttatgcg aagttttgct ttgaagaatt ttccgaagtg aagtattgga
     3241 tcaccattaa tgaaccgacc tcaatggccg ttcaacaata tacaagcggg acattcccgc
     3301 ctgccgagtc tggacgtttt gataagacgt tccaagctga acacaatcaa atggtggcgc
     3361 atgcccgtat cgttaatcta tataagtcaa tgcaattagg tggccaaatt ggaattgttc
     3421 atgcgttgca aactgtttat ccctacagtg atagcgctgt ggatcaccat gccgccgagt
     3481 tgcaggacgc tttggaaaat cgattgtatc tggatggcac attagctggc gaatatcacc
     3541 aagaaacttt ggcgctcgtg aaggaaattc tggatgccaa tcatcaaccg atgtttcaat
     3601 cgacaccgca ggaaatgaag gctattgatg aagcggccca tcagttagac tttgttgggg
     3661 tcaataatta tttcagcaag tggttgcgtg cctatcatgg caaatcagag accattcata
     3721 atggcgacgg caccaaaggc tcttcggtcg cgcggctgca aggcgttggc gaggaaaaac
     3781 tcccagatgg cattgagacg acagattggg attggtcgat ttatccacga ggaatgtacg
     3841 acatactgat gcggattcat aatgactatc cgttggtgcc agtgacctac gtgacagaaa
     3901 atggtattgg tcttaaagaa agcttgccag aaaatgcgac ccctgacacc gtgattgaag
     3961 atccaaaacg gattgattat gtaaagaaat atctgtccgc aatggctgat gctatccatg
     4021 atggcgcaaa tgtcaaaggt tacttcatct ggtcacttca agatcagttt tcttggacaa
     4081 acggttacag taaacgatat ggtctgttct tcgttgattt tccaacacaa aatcgttata
     4141 tcaaacaaag tgccgagtgg tttaagtctg tgagtgagac ccacatcatt ccggattaaa
     4201 aagtttttgg ctatttagac agcaaggatt gaatcggagg gaaaatgatg gctactaaag
     4261 aagaaatttc aatggttggt tttgcacttg tggcgtatgc aggcgatgct cgaacggctg
     4321 ctgtgcatgc acttgatgcc gctgaagcag gcgatttcga taaggcaaac gaattggttg
     4381 agaaggccca acaggatatc aatgaagccc acaaccaaca gacccagctt ctaagtcaag
     4441 aagctggcgg tgctgaaatg gatgtcacct ttatcatggt tcatggtcaa gatacgttga
     4501 tgacaaccat gttgctcatt gatgaaacac gctacatgat tcgcatgttt aagcggatca
     4561 aagaacttga gaacaagcag taaatgagtc atcatcattt ctcgataaaa tgatagtcga
     4621 atcgatcagc atttcctcca aatgacgaac tttgctgtga gcgcgtcctc aataattgaa
     4681 cagaagtaac ccaaaaacta ggcgcgtggc acatacaacg cctagttttt
//
