LOCUS       AM260479               10332 bp    DNA     linear   BCT 07-MAR-2015
DEFINITION  Ralstonia eutropha H16 chromosome 1.
ACCESSION   AM260479
VERSION     AM260479.1
DBLINK      BioProject: PRJNA13603
            BioSample: SAMEA3283071
KEYWORDS    complete genome.
SOURCE      Cupriavidus necator H16
  ORGANISM  Cupriavidus necator H16
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Cupriavidus.
REFERENCE   1
  AUTHORS   Pohlmann,A., Fricke,W.F., Reinecke,F., Kusian,B., Liesegang,H.,
            Cramm,R., Eitinger,T., Ewering,C., Potter,M., Schwartz,E.,
            Strittmatter,A., Voss,I., Gottschalk,G., Steinbuchel,A.,
            Friedrich,B. and Bowien,B.
  TITLE     Genome sequence of the bioplastic-producing 'Knallgas' bacterium
            Ralstonia eutropha H16
  JOURNAL   Nat. Biotechnol. 24 (10), 1257-1262 (2006)
   PUBMED   16964242
  REMARK    Erratum:[Nat Biotechnol. 2007 Apr;25(4):478]
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   Fricke,W.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2006) Fricke W.F., Institute for Microbiology and
            Genetics, Goettingen Genomics Laboratory, Grisebachstr. 8,
            Goettingen 37077, GERMANY
COMMENT     URL -- http://www.g2l.bio.uni-goettingen.de
            Contacts: Heiko Liesegang (hlieseg@gwdg.de).
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Cupriavidus necator H16"
                     /mol_type="genomic DNA"
                     /strain="H16"
                     /db_xref="taxon:381666"
                     /chromosome="1"
     gene            complement(1..723)
                     /gene="h16_A0310"
                     /locus_tag="H16_A0310"
     CDS             complement(1..723)
                     /gene="h16_A0310"
                     /locus_tag="H16_A0310"
                     /function="Transcription"
                     /inference="protein motif:COGS:COG2188"
                     /inference="protein motif:PFAM:PF00392"
                     /inference="protein motif:SMART:smart00345"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|GntR,DBD-SUPERFAMILY|0037767"
                     /protein_id="CAJ91461.1"
                     /db_xref="EnsemblGenomes-Gn:H16_A0310"
                     /db_xref="EnsemblGenomes-Tr:CAJ91461"
                     /db_xref="GOA:Q0KEW0"
                     /db_xref="InterPro:IPR000524"
                     /db_xref="InterPro:IPR011663"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR028978"
                     /db_xref="UniProtKB/TrEMBL:Q0KEW0"
                     /translation="MDQRLQALKPDEAEATPIYLQVARRLAAAIQAGQWRVGDALPSER
                     TLVDSLEISRVTARRALQVLAEEGAITRSRGAGTFVAPRPEQKAARLDNFSELARRRGM
                     TPASELVAFERRRATPQEAAALALQEGEEIVSLTRLRKADGQVFWMDVTTLALAVLPDA
                     SAIGESLYAYLERIGKPVLRVTERLRAIVAGEALAARLQIAPGEPLLHILRTGYTHGDQ
                     PVELTDGYCLNDFYELKQ"
     gene            1116..3668
                     /gene="nagF"
                     /locus_tag="H16_A0311"
     CDS             1116..3668
                     /gene="nagF"
                     /locus_tag="H16_A0311"
                     /EC_number="2.7.1.69"
                     /function="Carbohydrate transport and metabolism / Signal
                     transduction mechanisms"
                     /inference="protein motif:CDD:cd00210"
                     /inference="protein motif:COGS:COG1080"
                     /inference="protein motif:COGS:COG1925"
                     /inference="protein motif:PFAM:PF02896"
                     /inference="protein motif:TCDB:8.A.7"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9HXN5|4.A.1.1.15"
                     /protein_id="CAJ91462.1"
                     /db_xref="EnsemblGenomes-Gn:H16_A0311"
                     /db_xref="EnsemblGenomes-Tr:CAJ91462"
                     /db_xref="GOA:Q0KEV9"
                     /db_xref="InterPro:IPR000032"
                     /db_xref="InterPro:IPR000121"
                     /db_xref="InterPro:IPR001020"
                     /db_xref="InterPro:IPR001127"
                     /db_xref="InterPro:IPR006318"
                     /db_xref="InterPro:IPR008279"
                     /db_xref="InterPro:IPR008731"
                     /db_xref="InterPro:IPR011055"
                     /db_xref="InterPro:IPR015813"
                     /db_xref="InterPro:IPR018274"
                     /db_xref="InterPro:IPR023151"
                     /db_xref="UniProtKB/TrEMBL:Q0KEV9"
                     /translation="MAETSETLLLRAPVSGVLLPLAAVPDPVFADGMFGDGLAIDPLSD
                     TLVAPCDGTIVHLAATGHALTLQAPCGAQVLLHVGIDTVSLRGEGFAPCVAQGAAVRQG
                     DALIRFDVDRVAQQASSLVTVMAIANGDAFTVAGRTGAAQADAGRTVLLQVRAAGAAAP
                     AQEDHASAAEQRRTVTLACPGGLHARPAARAREAARGLDAAVALHYGGRQASLASLVSL
                     LGLAADEGAAVELVATGRDAGAALDAVAYELQRAGVGEEEPVPTVAAPIAQPGPGAGLP
                     ADGIPGVAAAPGIAIGTLHWWRETTPEPAEAGQGIAAEREALRAALAAIDAQLAHESEA
                     ATARRAGAEAGIFAMHRALLEDPALQAAAETMIAAGKSAGFAWREATRAEAAMLSAMAD
                     RRMAERAADLLDLGKRVLRALAPAGSAPVQLPAPAADGDVIVLAAGEFTPSDLTGLDRT
                     RVAALVMARGGPTSHAAIIARQLGIPCLVALGSALERLQDGTPVIADGDSARLHACPDA
                     HLLAAAASAVRLRAQQREADLRAAHAAASTRDGVPIEVAANIGSLDDARNAVAHGADAV
                     GLLRTELLFLHGDAAPAAADHTASCQAIADALQGRSAIVRTLDIGADKDAAWLHLPAED
                     NPALGLRGVRLAQVRPQLLDDQLRGLLAVRASGPLRILLPMVSEVGELLAWRERIRALA
                     AEAGTDPEIEVGVMIEVPSAALLADQLAEHADFFSIGTNDLTQYTLAIDRCQPELAARM
                     DGLHPAVLRMIAATTEGAARHGRWVGVCGALAGDPLAVPVLVGLGVTELSVDAGLVPGV
                     KARVRALSHADCRQRAGELLRLPSAAAVRDACRRYWPA"
     gene            3726..5435
                     /gene="nagE"
                     /locus_tag="H16_A0312"
     CDS             3726..5435
                     /gene="nagE"
                     /locus_tag="H16_A0312"
                     /EC_number="2.7.1.69"
                     /function="Carbohydrate transport and metabolism / Signal
                     transduction mechanisms"
                     /inference="protein motif:CDD:cd00212"
                     /inference="protein motif:COGS:COG1263"
                     /inference="protein motif:PFAM:PF02378"
                     /inference="protein motif:TCDB:4.A.1"
                     /note="14 TMHs"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9HXN4|4.A.1.1.15"
                     /protein_id="CAJ91463.1"
                     /db_xref="EnsemblGenomes-Gn:H16_A0312"
                     /db_xref="EnsemblGenomes-Tr:CAJ91463"
                     /db_xref="GOA:Q0KEV8"
                     /db_xref="InterPro:IPR001996"
                     /db_xref="InterPro:IPR003352"
                     /db_xref="InterPro:IPR010974"
                     /db_xref="InterPro:IPR013013"
                     /db_xref="InterPro:IPR018113"
                     /db_xref="UniProtKB/TrEMBL:Q0KEV8"
                     /translation="MKMDLLPRVQRLGATLMLPIAVLPVAGLLLRLGQPDVFDIKLMAE
                     AGNAVFANLALLFAIGVAVGFARDNNGAAALAGTIGYLVLTTVLKTIDKSLDMGVLAGI
                     VAGAVAGGLYNRYRNVALPPYLGFFSGKRFVPIVTALCCLLLGIVLAYAWAPVQAGINA
                     AGAWLTTAGSAGALVFGLLNRLLLVTGLHHLINTLAWFVFGNYADPATGAAVSGDLHRY
                     FAGDPGAGLFMTGFFPVMMFGLPAACLAMYHETPPARRALVGGMLFSMALTSFLTGITE
                     PIEFSFMFLAPVLYGLHALMTGLSMALCHALDIRLGFTFSAGAIDYVLGYGLSSRGWLA
                     IPLGLAYALVYYGLFRFFIRRFNLLTPGRDEVVPVAAAGGAAQPAAGSVAQQYVEALGG
                     PANLVVVDACTTRLRLNVADIGAVSEPRLKALGVRGVLKRPPNVVQVVIGPQAEQVAGD
                     IRAVLQQAPQATAVVAAAPAVATQASVPAAGAFDPAWWIDALGGAANIASVGVVALTRL
                     RVVVRERARVRADHLAGSQLMWIGDDTAHIAFGHAADGHAAAFERALQAMPT"
     gene            5552..6616
                     /gene="nagC"
                     /locus_tag="H16_A0313"
     CDS             5552..6616
                     /gene="nagC"
                     /locus_tag="H16_A0313"
                     /function="Carbohydrate transport and metabolism"
                     /inference="protein motif:CDD:cd00342"
                     /inference="protein motif:COGS:COG3203"
                     /inference="protein motif:TCDB:1.B.1"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|C0LZS0|1.B.1.4.2"
                     /protein_id="CAJ91464.1"
                     /db_xref="EnsemblGenomes-Gn:H16_A0313"
                     /db_xref="EnsemblGenomes-Tr:CAJ91464"
                     /db_xref="GOA:Q0KEV7"
                     /db_xref="InterPro:IPR001702"
                     /db_xref="InterPro:IPR002299"
                     /db_xref="InterPro:IPR023614"
                     /db_xref="UniProtKB/TrEMBL:Q0KEV7"
                     /translation="MKRIALTALLAASAGGACAQSAGTGITLYGRAVAGLDYVNGIARP
                     GGDVDTFRFGSNQYGVSFWGMRGAEDLGGGLRGVFNLEGMITSGTGRSDPLFNRRAQVG
                     LASNDWGTLLLGRAMSLTDDESGAIDPMGLQASSVATLVYGRNWGSRTNAVTYNSPVWG
                     GLSVRAQAGSDGVAGSFNSGKQLSAAVKYAQGNLSLTGIYEELRDTQGRMDNLYVNSRQ
                     YTLGGSYALNNLRLFAGYNLTVSSGDTVATPENPYAATRSQMAWVGANYRLLPALTLLS
                     GVYYATLNNGGGHGTLVTLGAKYDLSKRTSLYGTVASVFNSRNATFSVEASPDTMPGAG
                     ANQQGAYAGIIHLF"
     sig_peptide     5552..5611
                     /inference="protein motif:SignalP:2.0"
     gene            6659..7762
                     /gene="nagA"
                     /locus_tag="H16_A0314"
     CDS             6659..7762
                     /gene="nagA"
                     /locus_tag="H16_A0314"
                     /EC_number="3.5.1.25"
                     /function="Carbohydrate transport and metabolism"
                     /inference="protein motif:COGS:COG1820"
                     /codon_start=1
                     /transl_table=11
                     /product="CE9"
                     /protein_id="CAJ91465.1"
                     /db_xref="EnsemblGenomes-Gn:H16_A0314"
                     /db_xref="EnsemblGenomes-Tr:CAJ91465"
                     /db_xref="GOA:Q0KEV6"
                     /db_xref="InterPro:IPR003764"
                     /db_xref="InterPro:IPR011059"
                     /db_xref="UniProtKB/TrEMBL:Q0KEV6"
                     /translation="MKGNILTPAGWIFGEIHAGPDGRIARIEGVPASPEHNDAPYVLPG
                     FVDLLNHGGGGKDFMDGEAAVPVILRAHVRFGTTSLLGSTMTSPHDVLLPALQALNRAC
                     AQRPPGAARMLGVHLEGPYINPGKLGAQPDTSATATPEELEQYLGAAPLKVVTLAPELP
                     GHLDIIRLLVARGVRVQLGHTLGTYEDAVAALDAGASGFTHLFNAMTPLQHRAPGMVAA
                     ALAHAEFAELIPDLLHVHPGAIRAALRAIPRLYAVTDATAAAGMPDGVYHLGSQTVYKV
                     HGSVRLADGTLAGSVLTMDQALRNFVSIGLDLADASRRVSLYPAQYLGMSDRGMLAQGC
                     WADAVVLDRALLVRTVYVEGECCVENA"
     gene            7755..8786
                     /gene="nagB"
                     /locus_tag="H16_A0315"
     CDS             7755..8786
                     /gene="nagB"
                     /locus_tag="H16_A0315"
                     /EC_number="3.5.99.6"
                     /function="Carbohydrate transport and metabolism"
                     /inference="protein motif:COGS:COG2222"
                     /inference="protein motif:PFAM:PF01380"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SIS,STP|SIS"
                     /protein_id="CAJ91466.1"
                     /db_xref="EnsemblGenomes-Gn:H16_A0315"
                     /db_xref="EnsemblGenomes-Tr:CAJ91466"
                     /db_xref="GOA:Q0KEV5"
                     /db_xref="InterPro:IPR001347"
                     /db_xref="UniProtKB/TrEMBL:Q0KEV5"
                     /translation="MLEEALSAPAVVAAQLAQAPRVAALAAQLAAQAWPAALTVARGSS
                     DHAASYFATLVTRRLGVPVASLPMSTVTLHDSALRVAGQLAVAFSQSGRSPDLVETMQA
                     LRDRGAATVALVNAPDSPLAQACAHELPLLAGPEQSVAATKSYIAMLSMSALLVGHLEA
                     ARSGEHELVDACAGLPAALAQAAAQDWSALVPALRDVERMIVLGRGAGLAIAQEAALKL
                     KETSGIQAEAFSGAEVRHGPMEIIGDGYPLLVFAPRGPEQAGLLALAAEMRKRGARVLL
                     AAPPGTVDAELPLELPLVPAGHPLLDPVCAIQGFYVAAQALALARGRNPDQPQYLRKVT
                     ETR"
     gene            8845..10332
                     /gene="zwf1"
                     /locus_tag="H16_A0316"
     CDS             8845..10332
                     /gene="zwf1"
                     /locus_tag="H16_A0316"
                     /EC_number="1.1.1.49"
                     /function="Carbohydrate transport and metabolism"
                     /inference="protein motif:PFAM:PF00479"
                     /inference="protein motif:PFAM:PF02781"
                     /codon_start=1
                     /transl_table=11
                     /product="Glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="CAJ91467.1"
                     /db_xref="EnsemblGenomes-Gn:H16_A0316"
                     /db_xref="EnsemblGenomes-Tr:CAJ91467"
                     /db_xref="GOA:Q0KEV4"
                     /db_xref="InterPro:IPR001282"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR019796"
                     /db_xref="InterPro:IPR022674"
                     /db_xref="InterPro:IPR022675"
                     /db_xref="UniProtKB/TrEMBL:Q0KEV4"
                     /translation="MQTTRTASSSGTADARPLDLVIFGGAGDLSARKLLPSLYMCHRDG
                     NLPDGTRIIGVGRHRWDREAFVNFADESAQPFVEARYLEPAKWQTFLQRLDFVHVDASQ
                     SGDYPALAAQLRQEALRIYYMAMPPGLFAATCDNLASHGLIANDTRLVLEKPLGVDLAS
                     AIGIGEVVSRYFSEDRTYRIDHYLGKETVQNLMALRFGNSIFEPLWRTPFVRSVQITVA
                     ETVGVGTRGGFYDEAGAMRDMVQNHLLQLVSILAMEPPAALTSDAVRDEKLKVLRSLRP
                     MSPEDVRRNTVRGQYTAGAIAGELVRGYLQEEGIPPDSRTETFVAMRAELGTWRWNKVP
                     FYLRTGKRMQERVTEVVINFAEVPHSIFDAGSTLQPNRMVIRLQPEESVRLTLMVKQPG
                     EGMKLKPLSLALNLDSAFTTRRAEAYERLLLDVIRGRLALFVRRDELQAAWTWVDPILE
                     AWRQQDEGPRPYTAGTWGPAASSAFMAREGVQWSEEA"
ORIGIN
        1 ctactgcttc agctcgtaga aatcgttcag gcagtagccg tcggtcagtt cgaccggctg
       61 gtcgccatgg gtgtagccgg tgcgcaggat atgcagcagc ggctcgccgg gcgcgatctg
      121 caggcgcgcg gccagtgctt cgccggcgac gatcgcgcgc agcctttcgg tgacgcgcag
      181 caccggcttg ccgatccgct ccaggtaggc gtacagcgat tcgccgatgg cgctggcgtc
      241 gggcagcacg gccagtgcca gcgtggtgac atccatccag aagacctgcc cgtcggcctt
      301 gcgcaggcgg gtcaggctga caatctcttc cccttcctgc agcgccagcg ccgcagcctc
      361 ctggggcgtg gcgcggcggc gttcgaacgc caccagttcg ctggccggcg tcatgccgcg
      421 ccggcgcgcc agctcgctga agttgtccag ccgcgccgcc ttctgctcag ggcgcggcgc
      481 gacaaaggtg cccgcgccgc ggctgcgcgt gatcgcgcct tcctctgcca gcacctgcag
      541 cgcgcgccgc gccgtgacgc gtgaaatctc cagtgaatcc accagcgtgc gctcggacgg
      601 cagcgcgtcg ccaacccgcc attggccggc ctggatggcc gcggccagcc tgcgcgccac
      661 ttgcaggtag atcggcgtcg cctccgcttc gtccggcttg agagcctgca gccgttgatc
      721 cattgcgaat cctcgtaggt accagagtgt ggaggtgatg ataccacttt aggacatttg
      781 acgtcgatgg tgcggcattg cgtcatctgg gcgataacat cgccatcgct tcgctttacg
      841 tcaattggta ttgaaatggt tctgctgatt atggcgtcgg aaaatgctgc tgtgatttgg
      901 aaaaatctta cctatctccg tgggttatgg tgattaaggc gaatgtgagg accatccaag
      961 gactgttttg ccttgcctca ataaagatgc cacttagata caatattgtt gtagtggttt
     1021 tcactggtat ttttattgtc ggttgccgtc tatctttcgc ctgaccctgg cgccgtcgca
     1081 tttctgcacc ggccgccgcg acttctggaa gcaaaatggc tgagacgagc gaaaccctgt
     1141 tgctgcgggc tccggtgagc ggagtgctgc ttcccctcgc ggccgtgccg gacccggtct
     1201 ttgccgacgg catgttcggc gacggcctgg ccatcgaccc cctgagcgac acgctggtgg
     1261 cgccttgcga cggcaccatc gtccacctgg ccgccaccgg ccacgccctg acgctgcagg
     1321 ctccgtgcgg ggcgcaggtg ctgctccatg tcggcatcga tacggtgtcg ctgcgcggcg
     1381 aaggctttgc gccttgcgtg gcgcagggcg ctgccgtgcg ccagggggat gcgctgatcc
     1441 gtttcgatgt cgaccgtgtc gcgcagcagg cgtcgagcct ggtgacggtg atggcaatcg
     1501 ccaatggcga tgccttcacg gtcgccggca ggaccggcgc ggcgcaggcc gacgcgggcc
     1561 gcacggtgct gctgcaggtg cgtgccgcgg gtgccgcggc gcccgcgcag gaagaccacg
     1621 cgagcgcagc cgaacagcgg cgcacggtca cgctggcctg ccccggcggc ttgcacgcgc
     1681 ggcccgcggc acgggcgcgc gaagccgcgc gcggcctgga cgccgccgtc gcattgcact
     1741 acggcggccg gcaggcgagc ctggccagcc tggtgtcgct gctgggcctg gccgccgacg
     1801 agggcgccgc ggtcgagctg gtggcgaccg gccgcgatgc cggtgccgcg ctcgatgcgg
     1861 tcgcatacga actgcagcgc gccggcgtgg gcgaagaaga acccgtgccc acggtggccg
     1921 caccgattgc gcagcctggg ccgggcgccg gcctgccggc ggatggcatc cccggcgtgg
     1981 ctgccgcccc cggcatcgcg atcggcacgc tgcactggtg gcgcgagacc acgccggagc
     2041 cggccgaggc cggccagggg attgctgccg agcgcgaggc cctgcgcgcc gcgctggcgg
     2101 ccatcgacgc ccagctggcg catgagagcg aagccgccac cgcacgccgc gccggcgccg
     2161 aggccggcat cttcgccatg catcgcgcgc tgctggaaga cccggccctg caggccgcgg
     2221 ccgagaccat gatcgccgcc ggcaagagcg cgggctttgc ctggcgcgag gccacccggg
     2281 ccgaagccgc catgctgtcc gcgatggccg accgccgcat ggccgaacgc gctgccgacc
     2341 tgctggacct gggcaagcgc gtgctgcgtg cgctggcccc ggcgggcagt gccccggtcc
     2401 agctgcccgc accggccgcc gacggcgatg tcatcgtgct ggccgccggg gaattcacgc
     2461 cgtccgacct gaccggactg gaccgcaccc gggttgccgc actggtgatg gcgcgcggcg
     2521 gcccgacctc gcacgcggcc atcatcgcgc gtcagctcgg catcccttgc ctggtggctc
     2581 tgggcagcgc gctggagcgc ctgcaggacg gcacgccggt catcgccgac ggcgacagcg
     2641 cccgcctgca cgcctgcccc gatgcgcatt tgctggccgc cgccgccagc gccgtcaggc
     2701 tgcgcgcgca gcagcgcgag gccgacctgc gcgccgcgca cgccgcggcc agcacccgcg
     2761 acggcgtgcc tattgaggtg gccgcgaata tcggcagcct cgacgacgcc cgcaatgccg
     2821 tggcgcatgg tgccgacgcg gtcggcttgc tgcgcaccga actgctgttc ctgcacggcg
     2881 acgcagcccc cgcggcggcg gaccacaccg ccagctgcca ggccattgcc gatgccctgc
     2941 agggccgcag cgcgatcgtg cgcacgcttg atatcggcgc cgacaaggac gccgcctggc
     3001 tgcacctgcc tgccgaagac aaccccgccc tgggcctgcg cggcgtgcgc ctggcgcagg
     3061 tgcgcccgca actgctggac gaccagctgc gcggcctgct tgcagtgcgc gcgagcgggc
     3121 cgctgcgcat cctgctgccg atggtcagcg aggtcggcga actgctggcc tggcgcgaac
     3181 gcatccgtgc gctggcggcc gaagccggta ccgatcccga gatcgaggtc ggcgtgatga
     3241 tcgaagtccc ttccgccgcg ctgctggccg accagctggc cgagcacgcc gatttcttct
     3301 cgatcggcac caacgacctg acccagtaca ccctggccat tgaccgctgc cagccggagc
     3361 tggcggcacg catggatggc ctgcatcccg cggtgctgcg catgatcgcc gccaccaccg
     3421 agggcgccgc gcgccacggg cgctgggtag gcgtgtgcgg cgcgctggcg ggcgacccgc
     3481 tggccgtgcc ggtgctggtc gggctgggcg tgaccgagtt gtcagtcgac gccgggctgg
     3541 tgccgggcgt caaggcgcgc gtgcgcgcgc tctcgcatgc cgattgccgg cagcgcgcag
     3601 gcgaattgtt gcggctgccg tcagccgccg cggtgcgcga cgcgtgccgc cgctactggc
     3661 cggcataagc gccggtcgac cacaacccaa tacgcgccac gcgcgcacag attcggagaa
     3721 cgccaatgaa gatggacctg ctgcccaggg tgcaacgcct gggcgccacg ctgatgctgc
     3781 cgattgccgt gctgcccgtg gccggcctgc tgctgcgcct gggccagccc gacgtattcg
     3841 acatcaagct gatggccgag gccggcaacg cggtctttgc caacctggcc ctgctgttcg
     3901 cgatcggcgt ggcggtgggc tttgcgcgcg acaacaacgg cgcggcggca ctggccggca
     3961 ccatcggcta cctggtgctg accacggtgc tgaagaccat cgacaaatcg ctcgacatgg
     4021 gcgtgctggc cggcatcgtc gccggcgcgg tggcgggcgg gctctacaat cgctatcgca
     4081 atgtcgcgct gccaccctat ctgggcttct tttccggcaa gcgcttcgtt cccatcgtca
     4141 ccgcgctgtg ctgcctgctg ctgggcatcg tgctggccta tgcgtgggcg ccggtgcagg
     4201 ccggcattaa cgcggccggc gcgtggctga ccacggcggg ctcggccggc gccttggtgt
     4261 tcgggctgct gaaccggctg ctgctggtca ccgggctgca ccacctgatc aataccctgg
     4321 cgtggttcgt gttcggcaac tacgccgatc cggccacggg ggcggcggta tcgggcgacc
     4381 tgcaccgcta ctttgccggc gaccccggcg caggcctgtt catgaccggc tttttcccgg
     4441 tgatgatgtt cggcctgccc gcggcctgcc tggcgatgta ccacgagacg ccgccggcgc
     4501 ggcgcgcgct ggttggcggc atgctgttct cgatggcact gacctcgttc ctgaccggca
     4561 tcaccgagcc gatcgagttc agcttcatgt tcctggcgcc ggtgctgtac ggcctgcatg
     4621 cgctgatgac cggcctgtcg atggcgctgt gccatgcgct cgatatccgg ctgggcttca
     4681 ccttctcggc cggcgcgatc gactatgtgc tgggctacgg gctgtccagc cgcggctggc
     4741 tggcgattcc gctggggctg gcctatgcgc tggtctatta cgggctgttc cgcttcttta
     4801 tccgccgctt caacctgctg acgccgggcc gcgatgaagt ggtgcccgtg gcggccgccg
     4861 gcggtgccgc gcagccggcc gcgggcagcg tggcgcaaca gtatgtcgaa gcgctgggcg
     4921 gccctgccaa cctggtcgtg gtcgatgcct gcaccacgcg tctgcggctg aacgtggccg
     4981 acatcggcgc ggtgtccgag ccgcggctca aggcactggg cgtacgcggc gtgctcaagc
     5041 gcccgcccaa tgtggtgcag gtggtgatcg ggccgcaggc cgagcaggtt gccggtgata
     5101 tccgcgcggt gctgcagcag gcgccgcagg ccacggccgt ggtggctgct gcgcccgccg
     5161 ttgctaccca ggcgtccgtg ccggctgccg gtgctttcga tcccgcctgg tggatcgatg
     5221 cgctgggcgg cgccgccaat atcgcctcgg tcggcgtggt ggcactgacg cggctgcgcg
     5281 tggtggtgcg cgagcgcgcc cgggtgcgcg ccgaccacct tgcgggcagc cagctgatgt
     5341 ggatcggcga tgacaccgcc catatcgcct tcggccatgc ggcagacgga cacgctgccg
     5401 ccttcgagcg cgccctgcag gccatgccca cctgaccccc gccgccgcag ccgtgcggct
     5461 gcggtcgacc ttccccatcg gcaacaacaa cgacggccgc gacgacagcc gcgccggtgg
     5521 ataccgtctg cttatccagg agtcattgat cgtgaaacgc attgcactga cggcgctgct
     5581 cgcagcgtcg gccgggggcg cctgcgccca gtccgccggc accggcatca ccttgtatgg
     5641 ccgcgccgtg gccggcctcg actatgtcaa cggcatcgcc cgcccgggcg gcgacgtgga
     5701 caccttccgc ttcggcagca accagtacgg cgtcagcttc tggggcatgc gcggcgcaga
     5761 agaccttggc ggcggcctgc gcggggtctt caacctggag ggcatgatca cctccggcac
     5821 cgggcgctcc gatccgctgt tcaaccggcg cgcgcaagtg ggcctggcca gtaacgactg
     5881 gggcacgctg ctgctgggcc gggccatgag cctgaccgac gacgagagcg gcgcgatcga
     5941 cccgatgggc ctgcaggcca gctcggtcgc gacgctggtc tatggccgca actggggctc
     6001 gcgcaccaat gccgtgacct acaactcgcc ggtgtggggc gggctgagtg tgcgcgcgca
     6061 ggccggcagc gacggcgtgg cgggcagctt caacagcggc aagcagttga gcgcggccgt
     6121 caagtacgcg cagggcaacc tgagcctgac cggcatctac gaggaactgc gcgacacgca
     6181 gggccgcatg gacaacctgt atgtcaactc gcgccagtac acgctcggtg gcagctatgc
     6241 gctgaacaac ctgcgcctgt tcgccggcta taacctgacc gtgtcctcgg gcgataccgt
     6301 ggccacgccg gagaacccct acgccgccac gcgcagccag atggcatggg tcggcgccaa
     6361 ctaccgcctg ctgccggcgc tgacgctgct gtccggcgtc tactacgcca ccctgaacaa
     6421 tggcggcggg catggcacgc ttgtcacact gggcgcaaag tatgacttgt cgaagcgcac
     6481 ctcgctgtat ggcacggtgg cgtcggtgtt caactcgcgc aatgccacct tctcggtgga
     6541 ggcgtcgccg gacaccatgc cgggcgccgg ggcgaaccag cagggcgcct atgccggcat
     6601 catccacctg ttctgacggt ggctggccgg tgatctgaat gtattggaga gtattgccat
     6661 gaaaggaaac atcctgacgc cggccggctg gatcttcgga gagatccatg ccggcccgga
     6721 cggacgcatc gcgcgcatcg agggcgtgcc cgcctcgcct gagcacaacg atgccccgta
     6781 cgtgctgccg gggtttgtcg acctgctcaa ccacggcggc ggcggcaagg acttcatgga
     6841 tggcgaggcc gcggtgccgg tgatcctgcg cgcgcatgtg cgctttggca ccaccagcct
     6901 gctgggcagc accatgacct cgccgcacga cgtgctgctg cccgcactgc aggcgctgaa
     6961 ccgcgcctgc gcgcagcgcc cgcccggcgc ggcgcgcatg ctgggcgtgc acctggaagg
     7021 gccgtatatc aaccccggca agctgggcgc gcagcccgat acctcggcca ctgccacgcc
     7081 ggaagaactg gagcagtacc tcggcgccgc gccgctgaag gtggtgacgc tggcgcccga
     7141 actgccgggg catctggaca tcatccgcct gctggtcgcg cgcggcgtgc gcgtgcagct
     7201 gggccacacg ctgggcacct atgaggacgc ggtggccgcg ctggatgccg gcgccagcgg
     7261 cttcacccac ctgttcaacg cgatgacccc gctgcagcac cgcgcgcccg gcatggtggc
     7321 ggcagcgctg gcccatgccg agtttgccga gctgatcccc gacctgctgc acgttcatcc
     7381 gggcgcgatc cgcgccgcgc tgcgcgcgat tccgcggctg tatgcggtga cggacgccac
     7441 cgccgccgcg ggcatgccgg acggcgtgta ccacctgggc agccagaccg tctacaaggt
     7501 ccacggcagc gtgcgcctgg ccgatggcac gctggccggc agcgtgctga ccatggacca
     7561 ggcgctgcgc aacttcgtgt cgatcgggct ggacctcgcc gatgcgtcgc ggcgcgtttc
     7621 gctgtatccg gcccagtacc tgggcatgtc cgaccgcggc atgctggcac agggctgctg
     7681 ggccgacgcg gtcgtgctgg accgcgcgct cttggtgcgc accgtttatg tcgaaggaga
     7741 atgctgtgtc gagaatgctt gaagaggcgc tgagcgcgcc ggccgtggtg gccgcgcagt
     7801 tggcgcaggc gccgcgcgtg gccgcgctgg ctgcgcaact cgccgcgcag gcctggcccg
     7861 cggcgctgac cgtggcgcgc ggcagctcgg accatgccgc cagctacttc gccacgctgg
     7921 tcacgcgccg gctgggcgtg ccggtggcct cgctgccgat gtcgacggtg acgctgcacg
     7981 acagcgcgct gcgcgtggcc gggcagctgg cggtggcgtt ctcgcagtcg ggccgcagcc
     8041 ccgacctggt cgagaccatg caagcgctgc gcgaccgcgg cgccgccacc gtggcgctgg
     8101 tcaacgcgcc cgattcgccg ctggcgcagg cgtgcgcgca tgaactgccc ctgcttgccg
     8161 gccccgagca gagcgtggcc gcgaccaaga gctatatcgc catgctgtcg atgtcggcgc
     8221 tgctggtggg gcatctcgag gccgcacgca gcggcgagca cgaactggtc gatgcctgcg
     8281 caggcttgcc ggccgcgctg gcgcaagcgg ccgcgcagga ctggtcggcg ctggtgccag
     8341 cgctgcgcga cgtcgagcgc atgatcgtgc tgggccgcgg cgcgggcctg gcgatcgcgc
     8401 aggaagcggc gctcaagctc aaggagacct ccggcatcca ggccgaggct ttctccggcg
     8461 ccgaagtgcg gcatggcccg atggaaatca tcggcgacgg ctacccgctg ctggtgttcg
     8521 cgccgcgcgg accggaacag gcgggactgc tggcgctggc cgccgagatg cgcaagcgcg
     8581 gtgcgcgcgt gctgctggcg gcgcctcctg gcacggttga tgcggagctg cccctggagt
     8641 tgccgctggt gccggccggc catccgctgc tcgatccggt ctgcgccatc cagggcttct
     8701 atgtcgcggc gcaggcgctg gcgctggcac gcggccgcaa tcccgaccag ccgcagtacc
     8761 tgcgcaaggt gaccgagacg cgctgagccg gcccataccg ccccaatacc gccccatgcc
     8821 gccccggcaa gcaaggagtc cgtcatgcaa accacccgaa ccgcctcttc ttccggcact
     8881 gccgacgcgc ggccactgga cctcgtcatc ttcggcggcg ccggcgacct gtcggcacgc
     8941 aagctgctgc catcgctcta catgtgccac cgcgacggca acctgcccga cggcacccgc
     9001 atcatcggcg tgggccggca ccgctgggac cgcgaggcct ttgtcaattt tgccgatgaa
     9061 agcgcacaac cctttgtcga agcgcgctat ctcgagccgg ccaaatggca gaccttcctg
     9121 cagcggctgg atttcgtgca cgttgacgcg tcgcagtccg gggactatcc ggcgctggcc
     9181 gcgcaactgc gccaggaggc gctgcgcatc tattacatgg cgatgccgcc gggcttgttc
     9241 gcggccacct gcgacaacct tgccagccat ggcctgatcg ccaacgacac ccggctggtg
     9301 ctggaaaaac cgctgggcgt ggacctggcc tcggccatcg gcatcggcga ggtggtcagc
     9361 cgctacttca gcgaggaccg cacctaccgg atcgaccact acctgggcaa ggaaacggtg
     9421 cagaacctga tggcgctgcg cttcggcaat tcgatcttcg agccgctgtg gcgcacgccg
     9481 ttcgtgcgca gcgtgcagat caccgtggcc gagaccgtgg gcgtgggcac gcgcggcggc
     9541 ttctacgacg aggccggcgc catgcgcgac atggtgcaga accacctgct gcagctggtc
     9601 agcatcctgg cgatggagcc gccggcagcg ctcacctccg atgcggtgcg cgatgagaag
     9661 ctcaaggtgc tgcgctcgct gcggccgatg tcgcccgagg acgtacgccg caataccgtg
     9721 cgcggccagt acaccgcggg cgccatcgcc ggcgagctgg tgcgcggcta cctgcaggaa
     9781 gaaggcattc cgccagacag ccgcaccgag accttcgtcg cgatgcgcgc cgagctcggc
     9841 acgtggcgct ggaacaaggt gccgttctac ctgcgcactg gcaagcgcat gcaggagcgc
     9901 gtgaccgagg tggtgatcaa cttcgccgag gtgccgcatt cgatcttcga tgccggcagc
     9961 acgctgcagc ccaaccgcat ggtgatccgg ctgcagccgg aagagtcggt gcggctgacg
    10021 ctgatggtca agcagccagg cgagggcatg aagctgaagc cgctgagcct ggcgctgaac
    10081 ctggactcgg ccttcaccac ccggcgcgcc gaagcctacg agcgcctgct gctcgacgtg
    10141 atacgcggac ggctggcgct gttcgtgcgg cgcgacgaac tgcaggccgc ctggacctgg
    10201 gtcgacccga tcctggaagc gtggcgccag caggacgaag gcccgcgccc ctataccgcc
    10261 ggcacctggg gcccggcggc ttcgtcggcg ttcatggcgc gcgaaggcgt gcagtggtct
    10321 gaggaggcgt ga
//
