LOCUS       GU591787                7858 bp    DNA     linear   BCT 21-MAR-2011
DEFINITION  Roseburia inulinivorans DSM 16841 beta-fructosidase operon, complete
            sequence.
ACCESSION   GU591787
VERSION     GU591787.1
KEYWORDS    .
SOURCE      Roseburia inulinivorans DSM 16841
  ORGANISM  Roseburia inulinivorans DSM 16841
            Bacteria; Firmicutes; Clostridia; Clostridiales; Lachnospiraceae;
            Roseburia.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Scott,K.P., Martin,J.C., Chassard,C., Clerget,M., Potrykus,J.,
            Campbell,G., Mayer,C.D., Young,P., Rucklidge,G., Ramsay,A.G. and
            Flint,H.J.
  TITLE     Microbes and Health Sackler Colloquium: Substrate-driven gene
            expression in Roseburia inulinivorans: Importance of inducible
            enzymes in the utilization of inulin and starch
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 108 (Suppl. 1), 4672-4679 (2010)
   PUBMED   20679207
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Scott,K.P. and Martin,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2010) Microbial Ecology, Rowett Institute of
            Nutrition and Health, Greenburn Road, Aberdeen, Scotland AB21 9SB,
            UK
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Roseburia inulinivorans DSM 16841"
                     /mol_type="genomic DNA"
                     /strain="A2-194"
                     /isolation_source="fecal sample"
                     /host="Homo sapiens"
                     /culture_collection="DSM:16841"
                     /type_material="type strain of Roseburia inulinivorans"
                     /db_xref="taxon:622312"
                     /clone="I1"
                     /country="United Kingdom"
                     /collection_date="Dec-1997"
                     /collected_by="Jenny Martin"
                     /identified_by="Jenny Martin"
                     /note="strain coidentity: DSM 16841 = A2-194"
     operon          21..7392
                     /operon="beta-fructosidase"
     CDS             complement(21..1046)
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="ADF57363.1"
                     /translation="MAKKQVTFADIAEYTHFSKTTISRYFNHPDSLTLENQEKISQALE
                     DLGYKKNKLAKVLANGKSEFIGIIVPNLYLHYYSEMLTQLLSSYRDYHYKFLVFVSDNG
                     PDEEEQYIEELMSYQIEGLIVLSHTLSSEKLASYQIPVIAIEREAEHICSVTTDNYMGA
                     LQAASLLIRDHCDVLIHINADVPKIIPAYNRIRAFEDTCKEHHVPYELNLNVCGDSYQE
                     LFSQMKIIFADIDEKYPGQKKGVFLANDTYANMFLNLIFQKYGCLPDTYELVGFDNSPI
                     ASEAILPITTIGQQIDVIAHTSMELLVQQMEERKKRKPVPLAEPIHKQITPILIRRETT
                     N"
     CDS             1303..2241
                     /operon="beta-fructosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9KWT8|3.A.1.1.10"
                     /protein_id="ADF57364.1"
                     /translation="MKRGLKPKKSTGRQTLVSKVSYVKRNWQLYVFFLMPALVLTIIFK
                     YLPMGGLLIAFEDYNVIKGVFGSPWVGLEYFKRFLSSPDFMSYLLNTLKLSIYGLLWGF
                     PIPIILALLLNRIRKQGIKKKIQLLIYAPNFISVIVLCGMVRMFLSPVGPMNRILGITT
                     NWMTMPTAFRTIYIASGIWQGAGWASIMYTAALANASKELEEAAVMDGANLLQQIWYVE
                     LPAIKDIIVIQFILQAGNIMSIGFEKAYALQTDMNLPASEILSTYVYRIGLLNGDYGYS
                     TAVGLFNSVINVILLIFVNFVVKKINDGEGL"
     CDS             2254..3150
                     /operon="beta-fructosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9WYR1|3.A.1.1.39"
                     /protein_id="ADF57365.1"
                     /translation="METSRKIKYSGIDRLILGIAYALLGLFVLSIVIPLIYVVLASFMD
                     PTVLNNQGLSFRIKDWTLDAYRRVLENEMIWRGFFNSFFYSLAFTAISVFITLLAAYPM
                     SKKEFVGRNFFNVIFLITMFFGGGMIPTFILINRLHMVNTVWAILIPGAFNVWNMILAR
                     TYYQSIPKELREASSIDGANEIQHFFKIMIPVCKPIIAVLALWSFVGMWNSYFDALIYL
                     NDADLQPLQLVLRSILVQNTPQPGMIADIQSTAEMAKVAEQLKYATIVVSSLPLLIMYP
                     FFQKYFDKGIMVGSVKG"
     CDS             3170..4840
                     /operon="beta-fructosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute binding protein"
                     /protein_id="ADF57366.1"
                     /translation="MRRKINRMLSFGLALIMMFSIAGCGSNAQPQAEIDPDTPVSEVQF
                     PLKETAELSFITSAPATSTQDPNERIIFQRLEEQTNVHIKWTCFVSDQFSDKKNLALAQ
                     FGNLPDGLFNAGMSDYDLLRYAKQGIIIPLENLIDKYMPNLQAVFEKYPEYRTMCTAPD
                     GHIYSFPWIEQLGAGKEAIQAIGDIAYINKKWLDYLGLEIPTTTDELEQVLIAFRDHAD
                     ELQEEFDIEGDVIPMSFIINNGDQDPAILINGFGEGYGDTGDHFAVTDEGKVIYTAVQE
                     GYKEGIEWLHKLVTEDLIDPEAFTQEWSTYVAKGKNHRYGLCFSWDIANIDNNVDYVML
                     PALTGPTGVRNITRQNNSETSGFDRGRCVLTTSCRNTALAAAWIDQMYAPLQSPQNNWG
                     SYGETDSFNIFELSTNEKGDPMLKHMDLGDQSPVEVREAQSVNGPLAILNEYYGEYVTQ
                     PEDAKWRLDNMHETYLADMNSKYVYPNVFMSIEDTNKVSQYDTDIKKYAEQKKADWILN
                     GGIEKEWDSYLEKMEKYGLSDYLSIKQKYFDNYQKSLSE"
     CDS             4857..6374
                     /operon="beta-fructosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH32"
                     /protein_id="ADF57367.1"
                     /translation="MSNMLEKARKYETEKIAATDPQTKPLFHVSAPTGWINDPNGFSFY
                     DGQIHLFYQYHPYNREWGPMHWGHSVTCDMIHWEQLPAALAPDEEYDKAGCFSGSAIEA
                     DGKHVLVYTGVTRIKQPDGSEQDRQNQCIAFGDGKDYQKYDKNPVMTGEMLPEGCNRVD
                     FRDPKIWKENDTYYMIVGNKNENQIGQVVLCSSKNLTDWKFETILASNESGKIGTMWEC
                     PDFFQLGSKRVLICSPQDMKAQKYEFHNGHNSVYFLGDYDEKTHTFVKELPHALDYGMD
                     FYAPQTTELPDGRRIMIAWMKSWDACVIPNSQDWQGMMTLPRELELKDGKIWQTPVREI
                     EKYHKNPCRYDHAEINQETTLCGIEGRTIDLTVLLEEDEFQTFSMKLAANKEYETSFTY
                     HKAENMLEIDRTYCGVTKDVVCVRKLKISNPEGLKKMRFILDRQSIELFLNDGEQVATT
                     AICTPLEAQEIHFYSDKKIHINVEKYDIMSASEKNAFMHSANDEIIE"
                     /old_product="beta-fructosidase"
     gene            6436..7392
                     /gene="6-pfk"
                     /operon="beta-fructosidase"
     CDS             6436..7392
                     /gene="6-pfk"
                     /operon="beta-fructosidase"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|PfkB"
                     /protein_id="ADF57368.1"
                     /translation="MDVVALGELLIDFTENGISSQGNPLFEANPGGAPCNVLAMLTKLG
                     HKTAFIGKVGNDFFGKQLEQTIIDVGIDASGLQKDDDVHTTLALVHTYPDGDRDFSFYR
                     NPGADMMLTEAEVPEELIKGTRIFHFGTLSMTHEGVRNATKKALRAAKEAGAVISFDPN
                     LREPLWNSLDEAKEQVLYGLGQCDILKISDNEIQWLTGEEDFTKGVHWILERYHIPLIL
                     VSMGREGSRAYYKDLIVEVKPFIQKNTIETTGAGDTFCACVLHYILEHGLTDLTENDLK
                     EMLTFANAAASIITTRKGALRVMPEREEVEKLLSCFN"
ORIGIN
        1 gaccggtcta gagaatattt ttaatttgtt gtctcccggc ggataaggat aggcgtaatt
       61 tgtttatgga tcggttctgc cagcggaact ggttttctct ttttacgttc ttccatctgc
      121 tgcactaata attccatgga ggtgtgtgcg atcacatcga tttgctgtcc aatggttgtg
      181 atcggcaaaa tagcttcact ggcaatcgga gagttgtcaa accctaccag ttcatatgtg
      241 tcagggagac atccatattt ttgaaagatt agattcagga acatatttgc ataggtgtcg
      301 ttcgcaagaa agacaccttt tttttgtccg ggatattttt catcgatatc agcaaagatg
      361 attttcattt gtgaaaagag ctcctgataa gaatctccac acacattaag atttaattca
      421 taaggcacat gatgttcttt acaggtgtct tcaaatgcac gaattctgtt gtaggcagga
      481 ataatctttg gcacatctgc attaatatga atcagcacat cacaatggtc acggatcaaa
      541 agtgatgcag cctgcagagc tcccatataa ttatcagttg tgacactgca gatatgttct
      601 gcctcacgtt caattgctat gacaggaatc tgataagaag caagcttttc ggaagaaagc
      661 gtatggctta atacgatcag gccttcgatc tgataagaca tcaattcttc aatatactgc
      721 tcctcctcat ccggtccgtt atcactgaca aaaacaagaa atttataatg ataatcacgg
      781 tagctggaaa gaagctgggt aagcatttct gagtagtagt gaaggtaaag atttggtacg
      841 ataatcccga taaattcact tttcccattt gcgagcacct ttgcgagttt gttcttttta
      901 tatcctaaat cctcaagagc ctgagatatt ttttcctgat tttcaagtgt cagtgaatcc
      961 ggatgattaa aatatcttga aattgtagtt ttggaaaaat gcgtgtattc tgcaatgtca
     1021 gcaaatgtca cctgtttttt cgccataagt atctgtctcc tttacgcatt gtctattgta
     1081 tttatcagta tgttaaaata tgaatcgttt cgttacagtt tcatattaca catagtatag
     1141 caattaagtt ctggaatatc aataaaaaaa taactggtta cgtaactggt tactttaact
     1201 taaaaaagtg ttgacatcta ttggaaaata ggttattatc caattataaa gtaaccggtt
     1261 actttaccga ttatacaaca agactaaatg gaggaatagt gtatgaaaag agggttgaaa
     1321 ccaaaaaagt ctacaggcag acaaacgctt gtaagtaaag tttcctatgt caaaagaaac
     1381 tggcagctgt atgtgttttt cctgatgccg gcattggtgt taacaattat atttaagtat
     1441 cttccgatgg gtgggctgct catcgcattc gaagattata atgtgatcaa aggtgttttt
     1501 ggaagtccct gggtagggct ggaatacttc aaacgttttt tatcctcacc ggattttatg
     1561 agttatctgt taaacacatt aaagctcagc atttatggac tgctttgggg ttttccgatt
     1621 ccaattatcc tggcattatt actgaaccgg atccgtaagc aaggtataaa aaagaaaata
     1681 cagcttttaa tctatgcacc taactttatt tccgttattg tactctgtgg tatggtacgt
     1741 atgtttttat caccggttgg tccgatgaac cgtattctcg gtatcactac aaattggatg
     1801 acaatgccga ctgccttcag gacaatttat attgcaagcg gaatctggca gggagcagga
     1861 tgggcatcca tcatgtacac ggcagcttta gccaatgcca gtaaagaatt agaagaagcg
     1921 gctgttatgg atggtgcaaa tctgctgcag caaatctggt atgtcgagtt gccggccatt
     1981 aaagatatta ttgtaattca gtttatttta caggccggaa acattatgag tatcggattt
     2041 gaaaaagcat atgcattaca gacggatatg aaccttccgg catctgagat tttatcaact
     2101 tatgtgtatc ggatcggact tctgaacgga gattatggct actcaacggc tgtcggattg
     2161 ttcaactctg tcattaatgt tattttattg attttcgtaa attttgtagt aaagaaaata
     2221 aacgatggag aaggattata ggaggatgaa aagatggaaa catcaagaaa aataaaatat
     2281 tcgggaattg accgtctgat tttaggtata gcatatgcgc ttctcggttt gtttgttctt
     2341 tcgattgtta ttcctttgat atatgtggtt ctggcatctt ttatggatcc gactgtttta
     2401 aataatcagg gactgagttt tcggataaaa gactggacat tagatgcata ccggcgtgtt
     2461 ttagagaatg aaatgatatg gagaggattt tttaattcat ttttctactc gctggcgttt
     2521 actgcaattt ctgtttttat aacacttctt gcagcttatc cgatgtcaaa aaaagagttt
     2581 gtcggcagaa atttttttaa tgtaattttt ctgatcacta tgttttttgg tggtgggatg
     2641 atcccgacat ttatattaat caaccgttta catatggtga atactgtatg ggctatttta
     2701 atacccggag cattcaacgt atggaatatg attcttgcaa gaacttatta ccagtcaata
     2761 ccaaaggaac tcagagaggc gtcctccatt gatggtgcaa atgaaatcca gcattttttc
     2821 aaaattatga ttccggtgtg taaaccaatc atcgccgtac tggcactctg gtcttttgtt
     2881 ggaatgtgga acagctattt tgatgcactg atttatttga atgatgcaga tctgcagcca
     2941 ttacagcttg tgcttcgttc cattcttgta cagaatacac cacagcctgg aatgatcgct
     3001 gacatccaga gtacggctga gatggcaaaa gtagcggaac agttaaaata tgcgactatt
     3061 gttgtgtcaa gcttaccatt attaattatg tacccatttt tccagaaata tttcgataaa
     3121 ggaattatgg ttggatctgt aaaaggttaa agaaggggga acttcctata tgcgtagaaa
     3181 aataaataga atgttgtcat tcgggcttgc actgatcatg atgtttagta tagccggctg
     3241 cggaagcaat gcacagccac aggcagagat agatccggac actccggttt ccgaagtaca
     3301 gtttccgtta aaagaaactg cggaattatc ctttattacc agtgcaccgg caacctcaac
     3361 gcaggatccg aatgaaagga tcatttttca aagacttgag gaacagacga atgtgcatat
     3421 taaatggacg tgttttgtat cggatcagtt ttctgataaa aaaaatcttg cattagcaca
     3481 atttggcaat ctgccggatg gcttgtttaa tgccggaatg agtgattacg atctgcttcg
     3541 ttatgcaaag caggggatta ttattcccct tgaaaatctg atcgataaat atatgccaaa
     3601 tcttcaggct gtatttgaaa aatatccgga ataccgcaca atgtgtaccg caccggatgg
     3661 acatatatat tcatttccat ggatcgaaca gcttggtgcc ggtaaagaag ccatacaggc
     3721 catcggagat atcgcatata tcaataaaaa atggctggat tatcttggat tggaaatacc
     3781 cacaacaacg gatgaactgg aacaggtatt aattgcattc cgggatcatg cagatgaatt
     3841 gcaggaagaa tttgatattg aaggtgatgt gataccgatg tcctttatta tcaacaatgg
     3901 agatcaggat cccgccattc tgatcaatgg atttggagaa ggctatggtg ataccggtga
     3961 tcattttgca gtgacggatg aaggaaaggt catttacaca gctgttcagg aaggatataa
     4021 agaaggcatt gaatggctgc ataaattagt tacagaagat cttatagatc cggaagcatt
     4081 cacacaggaa tggtctacct atgttgcaaa aggtaaaaac caccgatatg gtctttgctt
     4141 ctcctgggat atcgcaaata ttgataataa tgttgattat gtaatgctcc ctgcacttac
     4201 aggtccgacg ggcgtgcgga atatcacaag acagaacaat agtgagacaa gcggatttga
     4261 cagaggacgt tgtgtactta cgaccagttg cagaaatact gcactggcag cagcatggat
     4321 cgatcagatg tatgcaccat tacagtctcc acagaacaat tggggcagtt atggtgagac
     4381 agattccttt aatatttttg aattatctac aaacgaaaaa ggtgacccga tgttaaagca
     4441 tatggattta ggcgaccagt ctccggtaga agtccgcgag gcacagtcag tcaatggtcc
     4501 gcttgccatc ttaaatgaat attatggtga gtatgtaaca cagccggaag atgcaaaatg
     4561 gcggcttgac aatatgcatg agacatattt ggcagatatg aacagtaaat acgtttatcc
     4621 gaatgtattt atgagtatag aagacacgaa taaggtgtcg caatatgaca cagatattaa
     4681 aaaatatgcc gaacagaaaa aagccgactg gattttaaat ggcggcatag aaaaggaatg
     4741 ggattcctat cttgaaaaaa tggaaaaata tggattatca gattatcttt ccattaagca
     4801 gaaatacttt gacaactatc agaaatcatt atcagaataa ttaggagaga aataatatga
     4861 gcaatatgtt agaaaaagcc agaaaatacg agacagaaaa aattgctgct acagatccgc
     4921 agacaaaacc tttatttcat gtttctgctc caaccggatg gatcaacgat ccaaatggat
     4981 tttcgtttta cgatggacag atacatctgt tttatcaata tcatccatat aacagggaat
     5041 ggggaccaat gcactggggg catagcgtta cgtgcgatat gatccactgg gaacagcttc
     5101 cagcggcact tgctccggat gaagaatatg ataaagccgg atgcttttca ggaagtgcga
     5161 tcgaagcaga tggtaaacat gtacttgtat atacaggagt aaccagaata aaacagcctg
     5221 acggaagtga gcaggatcgt cagaatcagt gtattgcatt tggtgatgga aaagattatc
     5281 agaaatatga caaaaatcct gtcatgaccg gagaaatgct tccagaggga tgcaacaggg
     5341 tagatttccg tgatccgaag atctggaaag aaaatgatac ctattatatg atcgttggca
     5401 ataaaaatga aaatcagatc ggacaggttg tgttatgttc aagcaaaaat ctgactgact
     5461 ggaaatttga aacaattctt gcttcaaacg aaagcggaaa aattggtaca atgtgggaat
     5521 gtccggactt ctttcagctg ggcagtaaaa gagtgctgat ctgttcaccg caggatatga
     5581 aagcgcagaa atatgagttt cataatggac ataacagtgt ttattttctt ggggactatg
     5641 atgaaaaaac acatacattt gtaaaagaat tgccgcatgc actagattat ggaatggatt
     5701 tttatgcacc gcagacaact gaactgccgg acggcagacg tattatgatt gcatggatga
     5761 aatcctggga tgcatgtgtg attccaaatt cacaggattg gcagggaatg atgacactgc
     5821 cgagggaatt ggaattaaaa gatggtaaaa tctggcagac accggtgcgt gaaattgaaa
     5881 agtatcataa aaatccatgt cgatatgatc atgcagaaat aaatcaggaa acaacactgt
     5941 gcggaattga aggtcggacg atcgacctta ccgttttact ggaagaggat gaatttcaaa
     6001 cattttccat gaaacttgcc gcaaataagg aatatgaaac aagctttact tatcataagg
     6061 ctgaaaatat gcttgagatc gatcgcactt attgcggtgt gacaaaagat gttgtctgcg
     6121 tgcggaaact gaagatttca aatccggaag ggcttaagaa aatgagattt attttggatc
     6181 gtcagtcgat cgaactattc ctgaacgatg gggaacaggt tgcgacaaca gccatctgca
     6241 cgccgttgga agcgcaggaa atccattttt acagtgacaa aaaaatacat attaatgttg
     6301 aaaagtatga tataatgagt gcaagtgaga agaatgcatt tatgcattcg gcgaacgacg
     6361 aaattattga gtgatgaaat cacgatatag taaaattata aaacaaaaac tactatcaga
     6421 cacggaggaa aaagtatgga tgttgtagct ttaggcgaat tactcattga ttttactgag
     6481 aatgggatca gcagccaggg aaatccatta tttgaggcaa atccgggtgg tgcaccatgc
     6541 aacgtactgg caatgttgac aaagctcgga cataagacag cttttatcgg aaaagtagga
     6601 aatgattttt tcggaaaaca gctggaacag acgattattg atgttgggat tgatgcatca
     6661 ggcttacaga aagatgatga cgtacataca acgttagcac tggtacatac ttatccggat
     6721 ggagacaggg atttttcgtt ttacagaaat cctggtgcag atatgatgct gacagaagcg
     6781 gaagtaccgg aagagttgat aaagggaaca cgaatctttc attttggcac actgtccatg
     6841 acacacgaag gtgtacgaaa tgcaacaaaa aaagcacttc gtgctgcaaa agaagccggt
     6901 gctgtcattt cctttgatcc gaatctgcgc gagccgctct ggaactcctt agatgaagca
     6961 aaagaacagg tgctttacgg attaggacag tgtgacattt taaagatttc tgataacgag
     7021 attcagtggc tgaccggaga agaagatttc acaaagggtg tacattggat ccttgagaga
     7081 tatcatattc cattgatcct tgtatccatg ggcagggaag gaagccgtgc ttattataaa
     7141 gatcttattg tagaagtgaa accttttata cagaagaata cgatagagac aaccggagcc
     7201 ggagatacct tctgtgcatg tgttctccat tatattttag agcatggact taccgatctt
     7261 acagagaatg acctgaaaga gatgctgact tttgcaaatg cagcggcatc cattatcact
     7321 acacgcaaag gcgcactccg cgtaatgccg gaacgggaag aggttgaaaa attgttatcc
     7381 tgtttcaatt gatactgtgt atacaatata tgagcggcag attaccgctc atatattgta
     7441 taatgatgtt ttctaagcaa ctgtactgct gcatctaatg atattctgtc atacatttcc
     7501 agacgtaata ctccatcttc aaactcacgg ttgtgaatga tgccgatatt tttaatactc
     7561 agatgattac ttgcaagtat tgtagcaact gtcgcaatac caccagcctc atctaaaaga
     7621 tctaaataca gttcatatat ttctttgatc ggacctttgt tcgtgatcgc aattgaatcc
     7681 ctgtaatctt tggcggactg aaaatcctga agcagtgcat ctgcatcaga agataaaata
     7741 tcgtgtttgg ttttctctaa catttcctca taggcatcga tcagccgcac gatctgttca
     7801 ctgttggact ggcagatatt ctgccacata acaggagagg aggaagcgat tctggtca
//
