LOCUS       X81461                  4885 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Escherichia coli vacJ gene, yfdC gene, cscB gene, cscK gene, cscA
            gene, cscR gene, dsDX gene, tRNA-Arg gene and partial b2345 gene,
            strain EC3132.
ACCESSION   X81461
VERSION     X81461.2
KEYWORDS    b2345 gene; cscA gene; cscB gene; cscK gene; cscR gene;
            D-fructokinase; dsDX gene; Lengeler J.W; lipoprotein precursor;
            putative D-serine permease; sucrose hydrolase; sucrose permease;
            sucrose specific transcriptional regulator; transfer RNA-Arg;
            transporter protein; tRNA-Arg gene; vacJ gene; yfdC gene.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Jahreis,K., Bentler,L., Bockmann,J., Hans,S., Meyer,A.,
            Siepelmeyer,J. and Lengeler,J.W.
  TITLE     Adaptation of sucrose metabolism in the Escherichia coli wild-type
            strain EC3132
  JOURNAL   J. Bacteriol. 184 (19), 5307-5316 (2002)
   PUBMED   12218016
REFERENCE   2
  AUTHORS   Bockmann,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-1994) J. Bockmann, Institute of Anatomy,
            Westfaelische Wilhelms-University of Muenster, Vesaliusweg 2-4,
            48149 Muenster, FRG
  REMARK    revised by [3]
REFERENCE   3  (bases 1 to 4885)
  AUTHORS   Bockmann,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2002) J. Bockmann, Institute of Anatomy,
            Westfaelische Wilhelms-University of Muenster, Vesaliusweg 2-4,
            48149 Muenster, FRG
COMMENT     On May 4, 2002 this sequence version replaced X81461.1.
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC3132"
                     /db_xref="taxon:562"
     gene            complement(1..1248)
                     /gene="cscB"
     CDS             complement(1..1248)
                     /gene="cscB"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P30000|2.A.1.5.3"
                     /protein_id="CAA57217.1"
                     /db_xref="GOA:P30000"
                     /db_xref="InterPro:IPR000576"
                     /db_xref="InterPro:IPR016196"
                     /db_xref="InterPro:IPR018457"
                     /db_xref="InterPro:IPR020846"
                     /db_xref="InterPro:IPR022814"
                     /db_xref="UniProtKB/Swiss-Prot:P30000"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLTG
                     TELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLLQS
                     NFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFFAGI
                     FFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRNFWVF
                     VIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAIIPFFV
                     NRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKYSVANFD
                     KRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLLFGIFFLS
                     KKREQIVMETPVPSAI"
     gene            complement(1310..2233)
                     /gene="cscK"
     CDS             complement(1310..2233)
                     /gene="cscK"
                     /codon_start=1
                     /transl_table=11
                     /product="D-fructokinase"
                     /protein_id="CAA57218.2"
                     /db_xref="GOA:P40713"
                     /db_xref="InterPro:IPR002139"
                     /db_xref="InterPro:IPR002173"
                     /db_xref="InterPro:IPR011611"
                     /db_xref="UniProtKB/Swiss-Prot:P40713"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGTS
                     GFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPS
                     ADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDL
                     WQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLVTKGA
                     EGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQ
                     RCGALAVTAKGAMTALPCRQELESEK"
     protein_bind    2310..2331
                     /note="putative"
                     /bound_moiety="cAMP-CrpA"
     protein_bind    2339..2350
                     /note="putative; cscKo"
                     /bound_moiety="CscR"
     protein_bind    2351..2372
                     /note="putative"
                     /bound_moiety="cAMP-CrpA"
     protein_bind    2403..2414
                     /note="putative; cscoA"
                     /bound_moiety="CscR"
     gene            2449..3882
                     /gene="cscA"
     CDS             2449..3882
                     /gene="cscA"
                     /codon_start=1
                     /transl_table=11
                     /product="GH32"
                     /protein_id="CAA57219.1"
                     /db_xref="GOA:P40714"
                     /db_xref="InterPro:IPR001362"
                     /db_xref="InterPro:IPR006232"
                     /db_xref="InterPro:IPR008985"
                     /db_xref="InterPro:IPR013148"
                     /db_xref="InterPro:IPR013189"
                     /db_xref="InterPro:IPR018053"
                     /db_xref="UniProtKB/Swiss-Prot:P40714"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLIW
                     FNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSAVD
                     DNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPK
                     VWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPDFFSL
                     GDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQS
                     FVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEAESLRQQ
                     HQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGAGMRLYIDNQSERLVL
                     WRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEERELS
                     LYASHGVAVLQHGALWQLG"
     gene            complement(3890..4885)
                     /gene="cscR"
     CDS             complement(3890..4885)
                     /gene="cscR"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="CAA57220.1"
                     /db_xref="GOA:P62604"
                     /db_xref="InterPro:IPR000843"
                     /db_xref="InterPro:IPR001761"
                     /db_xref="InterPro:IPR010982"
                     /db_xref="UniProtKB/Swiss-Prot:P62604"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLNY
                     VPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFSED
                     DAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPDDYT
                     AQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHMATGD
                     DHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMGFDNLV
                     GVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
ORIGIN
        1 ctatattgct gaaggtacag gcgtttccat aactatttgc tcgcgttttt tactcaggaa
       61 gaaaatgcca aatagcaaca tcaggcagac aatacccgaa attgcgaaga aaactgtctg
      121 gtagcctgcg tggtcaaaga gtatcccagt cggcgttgaa agcagcacaa tcccaagcga
      181 actggcaatt tgaaaaccaa tcagaaagat cgtcgacgac aggcgcttat caaagtttgc
      241 cacgctgtat ttgaagacgg atatgacaca aagtggaacc tcaatggcat gtaacagctt
      301 cactaatgaa ataatccagg ggttaacgaa caacgcgcag gaaaggatac gcaacgccat
      361 aatcacaaca ccgataagta atgcattttt tggccctacc cgattcacaa agaaaggaat
      421 aatcgccatg cacagcgctt cgagtaccac ctggaatgag ttgagataac catacaggcg
      481 cgttcctaca tcgtgtgatt cgaataaacc tgcataaaag acaggaaaga gttgttgatc
      541 aaaaatgtta tagaaagacc acgtccccac aataaatatg acgaaaaccc agaagtttcg
      601 atccttgaaa actgcgataa aatcctcttt ttttacccct cccgcatccg ccgctatgca
      661 ctggtgatcc ttatctttaa aacgcatgtt gatcatcata aatacagcgc caaatagcga
      721 gaccaaccag aagttgatat ggggactgat actaaaaaat ataccggcaa agaacgcgcc
      781 aatagcatag ccaaaagatc cccaggcgcg cgctgttcca tattcgaaat gaaaatttcg
      841 cgccattttt tcggtgaagc tgtcaagcaa accgcatccc gccagatacc ccaggccaaa
      901 aaagagcgcc cccagaatta gacctacaga aaaattgctt tgcagtaacg gttcataaac
      961 gtaaatcata aacggtccgg tcaagaccag aatgaaactc atacaccaga tgagcggttt
     1021 cttcagaccg agtttatcct gaacgatgcc gtagaacatc ataaatagaa tgctggtaaa
     1081 ctggttgacc gaataaagtg tacctaattc cgtccctgtt aatcctagat gtcctttcag
     1141 ccaaatagcg tataacgacc accacagcga ccaggaaata aaaaagagaa atgagtaact
     1201 ggatgcaaaa cgatagtacg catttctgaa tggaatattc agtgccataa ttacctgcct
     1261 gtcgttaaaa aattcatgtc ctatttagag ataagagcgg cctcgccgtt tacttctcac
     1321 tttccagttc ttgtcgacat ggcagcgctg tcattgcccc tttcgctgtt actgcaagcg
     1381 ctccgcaacg ttgagcgaga tcgataattc gtcgcatttc tctctcatct gtagataatc
     1441 ccgtagagga cagacctgtg agtaacccgg caacgaacgc atctcccgcc ccagtgctat
     1501 cgacacaatt cacagacatt ccagcaaaat ggtggacttg tcctcgataa cagaccacca
     1561 ccccttctgc acctttagtc accaacagca tggcgatctc atactctttt gccagggcgc
     1621 atatatcccg atcgttctgt gtttttccac tgataagtcg ccattcttct tccgagagct
     1681 tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa gcggagcaaa tgctcgtctt
     1741 gccatagatc ttcacgaata ttgggatcga agctgacaaa acctccggca tgccggatcg
     1801 ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc agacaacgca attgaacaga
     1861 gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt cgtctctaaa aaaagatcgg
     1921 cactggggcg gaccataaac gtaaatgaac gttctccttg atcgttcaga tcgacaagca
     1981 ccgtggatgt ccggtgccat tcatcttgct tcagatacgt gatatcgaca ccctcagtta
     2041 gcagcgttct ttgcattaac gcaccaaaag gatcatcacc gacccgacct ataaacccac
     2101 ttgttccgcc taatctggcg attcccaccg caacgttagc tggcgcgccg ccaggacaag
     2161 gcagtagccg cccgtctgat tctggcaaga gatctacgac cgcatcccct aaaacccata
     2221 ctttggctga catttttttc ccttaaattc atctgactta cgcatagtga taaacctctt
     2281 tttcgcaaaa tcgtcatgga tttactaaaa catgcatatt cgatcacaaa acgtcatagt
     2341 taacgttaac atttgtgata ttcatcgcat ttatgaaagt aagggacttt atttttataa
     2401 aagttaacgt taacaattca ccaaatttgc ttaaccagga tgattaaaat gacgcaatct
     2461 cgattgcatg cggcgcaaaa cgcactagca aaacttcacg agcgccgagg taacactttc
     2521 tatccccatt ttcacctcgc gcctcctgcc gggtggatga acgatccaaa cggcctgatc
     2581 tggtttaacg atcgttatca cgcgttttat caacatcacc cgatgagcga acactggggg
     2641 ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca tgagcctatt
     2701 gcgctagcgc caggagacga gaatgacaaa gacgggtgtt tttcaggtag tgctgtcgat
     2761 gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg tgcaggtaat
     2821 gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat tcatttcgag
     2881 aaacagggtg tgatcctcac tccaccagaa ggcatcatgc acttccgcga tcctaaagtg
     2941 tggcgtgaag ccgacacatg gtggatggta gtcggggcga aagacccagg caacacgggg
     3001 cagatcctgc tttatcgcgg cagttcattg cgtgaatgga ctttcgatcg cgtactggcc
     3061 cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag ccttggcgat
     3121 cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag ttatcgaaat
     3181 cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt tgcacaatcc
     3241 gggcatttta ctgaacttga taacgggcat gacttttatg caccacaaag ctttgtagcg
     3301 aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc aatgccctca
     3361 aaacgtgaag gctgggcagg ctgcatgacg ctggcgcgcg agctatcaga gagcaatggc
     3421 aaactcctac aacgcccggt acacgaagct gagtcgttac gccagcagca tcaatctatc
     3481 tctccccgca caatcagcaa taaatatgtt ttgcaggaaa acgcgcaagc agttgagatt
     3541 cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca gctcggcgct
     3601 ggaatgcggc tgtatattga taaccaatct gagcgacttg ttttgtggcg gtattaccca
     3661 cacgagaatt tagatggcta ccgtagtatt cccctcccgc agggtgacat gctcgcccta
     3721 aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga ggcggtgatg
     3781 agtagccgaa tatatccgca gccagaagaa cgggaactgt cgctctatgc ctcccacgga
     3841 gtggctgtgc tgcaacatgg agcactctgg caactgggtt aacataatat caggtggaac
     3901 aacggatcaa cagcgggcaa gggatccgcg tcactcttcc cccttcacga ccttcaataa
     3961 tatgcaatgc agcttcccgc ccgataatgt catgtggaag ctgaattgtg gtcagcggcg
     4021 gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc cattacggcg acatcctgcg
     4081 ggatacgtac ccccttcgcc aaaagaacct gataagccac aaaggctgcg cgatcgttac
     4141 cacatatcag aacatcaaaa tctggtttgc ccgatttgaa gtgggcattg agtaaacttg
     4201 cgagatcggt gtagtgatca tcacctgttg ccatgtgaaa ttgtttcacc tcagccagat
     4261 ctcgtccagc atcacgccag gcctgctcaa atccctgccg acgataccct gttgccaacg
     4321 cactttccgg tagccagaag cataacggtt gacgatagcc cgccgcgagc aaatgctgtg
     4381 ttgattcata ttgtgcagtg taatcatcag ggatataact gggtaacgct gggtcatccg
     4441 ccacacagtt cgccaataca atattttcac catacagaga ctcaggcagc gtgatatgtc
     4501 gcagccccat tgtagtatag ataatgccat ccggacggtg ggcaagcagc tgacgtgccg
     4561 cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa actattccag ccgaactcgc
     4621 tggcggtttg ctcaatggca agcagaatat caacagagaa aggagtggta gcagtgtcct
     4681 gcgccagcac ggcgagagtc gacggcttac gtccttgagc gcgcatctta cgggcggaaa
     4741 gatcaggaac ataattcagg gtctggattg cctgcaatac gcggtcacgc gttgcaggac
     4801 gcacagattc tgcattatgc atcacccggg agactgtcat catcgacact cccgccaggc
     4861 gtgcgacatc ctttaatgaa gccat
//
