LOCUS       CP016359               15857 bp    DNA     linear   BCT 13-JAN-2017
DEFINITION  Gramella flava JLT2011, complete genome.
ACCESSION   CP016359
VERSION     CP016359.1
DBLINK      BioProject: PRJNA325700
            BioSample: SAMN05231554
KEYWORDS    .
SOURCE      Gramella flava JLT2011
  ORGANISM  Gramella flava JLT2011
            Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Gramella.
REFERENCE   1  (bases 1 to 15857)
  AUTHORS   Tang,K.
  TITLE     Multi-omics approach to identify versatile polysaccharide
            utilization systems of a marine flavobacterium Gramella flava
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15857)
  AUTHORS   Tang,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2016) State Key Laboratory for Marine
            Environmental Science, Institute of Marine Microbes and Ecospheres,
            Xiamen University, Zhoulongquan Building A2-319, Xiamen 361005,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: HGAP v. 2
            Genome Coverage       :: 25x
            Sequencing Technology :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            Source DNA is available from State Key Laboratory for Marine
            Environmental Science, Institute of Marine Microbes and Ecospheres,
            Xiamen Univeristy, Xiamen, China.
FEATURES             Location/Qualifiers
     source          1..15857
                     /organism="Gramella flava JLT2011"
                     /mol_type="genomic DNA"
                     /strain="JLT2011"
                     /isolation_source="marine water"
                     /culture_collection="CGMCC:1.12375"
                     /culture_collection="LMG:27360"
                     /type_material="type strain of Gramella flava"
                     /db_xref="taxon:1229726"
                     /country="Pacific Ocean"
                     /collection_date="2010-11-30"
     gene            complement(1..2169)
                     /locus_tag="GRFL_0296"
     CDS             complement(1..2169)
                     /locus_tag="GRFL_0296"
                     /codon_start=1
                     /transl_table=11
                     /product="GH127|CBM13"
                     /protein_id="APU67020.1"
                     /translation="MSKLTSWISIFIAIVCLSCNSEKKQEQTLSIAKIPDTSATNTNYV
                     SNQKPLASSVLIKLPVGAVKPNGWLKEMMKRQANGLMGNLGEISAWLQEKDNAWLAEDG
                     KGFWGWEEVPYWLKGYGNTAYILGDEKMITETEKWIEAAIASQREDGNFGPERLGEDGT
                     QDFWANMIMLYCLQSYYEYSGDDRVIDLMTNYFKFQNNVPDEEFLSGSHYWQKVRGGDN
                     LHSVVWLYNRTGDEFLLELMEKIHRNTSNWSKRDNSIDDIHNWKETRDNMDYPGWYSNL
                     IDWHNVNVAQAFREPAQYYQLSHDEKDLKATYDNFKIVREHFGQVPGGMYGADENARPG
                     YDDPRQGIETCGVVEQMNSNESLLRITGDTFWADHTEEVAFNTFPATMMPDMKSLRYIT
                     SPNMVLNDDQNHRPGIMNDGPFLMMNPFSSRCCQHNHGQGWPYMVENLWMATPDNGLAA
                     VIYGPSTVSAKVADGQEVEISTETHYPFEQAIKFSISTSEKGTAFPLYFRIPSWAQEAS
                     VSINGTKTETAPEAGKYVRIDRNWQNGDVVTLDLPKKLEMKKWENNHNSVSVNYGPLSF
                     SLKIGEEYTKKQSDETAVWDSKWQEGADTKKWPSYEIHPTTDWNYGLLLEDDVTNSFTI
                     EERDWPENDFPFTPETTPIVLKAKAKQIPQWTIDQYGLAGELQESPVKSSEETETVELI
                     PMGAARLRISSFPVIGEGENANEWKQTE"
     gene            complement(2188..4629)
                     /locus_tag="GRFL_0297"
     CDS             complement(2188..4629)
                     /locus_tag="GRFL_0297"
                     /codon_start=1
                     /transl_table=11
                     /product="Glutaminase A"
                     /protein_id="APU67021.1"
                     /translation="MIKKLVFITAIIFSGILTAQQRQAPAYPLVTHDPNFSIWSFGDKI
                     NEQSTVHWTGTDQSLVGLIKVDGKVYRFLGNEPKNYVDVLSAADAGEYTVMATEDEPKS
                     GWQKADFDDSNWKSTKAPFGDEHDYVTKWESEDLWVRRKFDLKNSNFDDVFLKLHHDDN
                     IVVYLNGEKILEKTGWNDQYDYFPISEEVKSKLKKKDNILAIHIKNTAGGRFLDAGIVE
                     VKKPKTQVELAEQTGLDFTATQTKYMLTAGGVDVNLTFTSPLLMDDLDLLARPVSYISV
                     KTKPNDGQIHDVQVYFGASSLIASNERSQLMETESGSTSALNFLKAGTKEQPILEKKGD
                     NLRIDWGYMYVAVAKDANAKQNITKGTDASKEFMSNSMSASVRDGKSLMLNTIFPAEKL
                     SEEKEHLIMLGYDDIYSINYFGNQLRPWWNLDGTNSIQNELEKAYAEYDEVIEKCEDFN
                     EELHEDGVEAGGEEYAKILEIAYRQSIAAHKLTKSPDGEILFMSKENFSNGSINTVDIT
                     YPSAPMYLLYNPDLLKGMMNGIFYYSESGKWKKPFPAHDLGTYPIATGQTYGEDMPVEE
                     SGNMVVLAAAITKAEGNADYAKKHWESLTVWANYLTEAGLDPANQLSTDDFSGHLARNA
                     NLAAKAIVGVGGYGYVAKQLGKDDVAEEYTQKAKEMAKEWMELADAGDHYALTYNDKNT
                     WSQKYNLVWDKVLDLEIFPQEVFDTELKFYDTKQNQYGLPLDNRADYTKSDWILWTATM
                     ADDQATFEKFADPVYEFATETKNRVPMSDWHFTTSGDQRGFQARSVIGGYFIKLLRDEW
                     E"
     gene            complement(4638..5780)
                     /locus_tag="GRFL_0298"
     CDS             complement(4638..5780)
                     /locus_tag="GRFL_0298"
                     /EC_number="5.1.3.3"
                     /codon_start=1
                     /transl_table=11
                     /product="Aldose 1-epimerase"
                     /protein_id="APU67022.1"
                     /db_xref="GO:0004034"
                     /translation="MISFTSCKDDKKEDTAESTKVDSTAVSEMELQAKDFDTLIDDKKV
                     SLYWLKNDSIKAAFTNYGGRLVGLWVPDKNGKMTDVVVGMNSAEGFANATEPYFGATIG
                     RVGNRIAKGKFKLNGEEYTIPQNNNGNTLHGGKKGFQYVVWDGEKRNDSTLVLHYKSPD
                     MEEGFPGNLDVTVTYSLTSDRSVMMEYEATTDKATPVNLTNHAFFNLNGEGSGSILQHE
                     VQIEADKFTPVDGGLIPSGELKEVAGTPFDFTQPHTIGERIETDNEQLKNGGGYDHNFV
                     LTEAQTGDLNKAATVTGDQSGIVMDIFTQEPGLQFYSGNFMQSKNTFKSGAKDEYRTAF
                     AMETQHFPDAPNQPEFPQITLEPGDTYHTVSEYKFSTTNQ"
     gene            complement(5809..6909)
                     /locus_tag="GRFL_0299"
     CDS             complement(5809..6909)
                     /locus_tag="GRFL_0299"
                     /EC_number="3.2.1.55"
                     /codon_start=1
                     /transl_table=11
                     /product="GH43|GH43_26"
                     /protein_id="APU67023.1"
                     /translation="MSKGIVTKIVLLGALAVVSCKDGNSDVAVNENTPKVSEKTTFTNP
                     LKSSGADPYSWYKDGYYYYTNTLGNKLAIWKTKDLSEIGNAEMKVIWTPPENTMYSRNI
                     WAPQVQFLEGKWYMYFAADDGDNQNHRMYVLENSSEDPMTGEWEFKGKVAAPSDKWAID
                     ADIYEFQDQLYMIWSGWEGDENGRQDIFIAKMSNPYTIEGKRIKISSPTFDWERNYGNG
                     PEVLVNEGPQFLEHGDNLFIVFSASGCWTDNYSLGLLKLTGDDLLDAENWEKDPEPYFS
                     QSPENGVYGPGHNSFFKSPDGTEDWILYHANPNSGDGCGGNRSPRMQKITWNADGTPDF
                     GTPVAPGTELELPSGTSEIKNTENEN"
     gene            complement(6918..8738)
                     /locus_tag="GRFL_0300"
     CDS             complement(6918..8738)
                     /locus_tag="GRFL_0300"
                     /EC_number="3.2.1.23"
                     /codon_start=1
                     /transl_table=11
                     /product="GH2"
                     /protein_id="APU67024.1"
                     /db_xref="GO:0004565"
                     /translation="MNSIIKNITILCLVLLSQVTMQAQWKPAGDKIKSKWADEVNPDKP
                     LNEYPRPIMERPDWKNLNGLWEYSIQQKGKAKPQNFDGDILVPFAVESSLSGVMKTVGA
                     ENELWYNTTFTVPGDWNGKKIKLNFDAVDWQADVWINDIKLGSHSGGYEPFSFDITPFL
                     NKDGEQKLTVKVWDPSDEGPQPRGKQVKNPEGIWYTPVTGIWQTVWLEPVAEKSLQNLR
                     FTPDIDNNTVKVSAEGSNSAYGDMIEVVVYDGNKEISTTKSSLDQEFSIHVNDPKLWSP
                     DSPFLYRTEVKLISNGEVQDEVKSYFAMRKISSKRDENGIIRMQLNNEDIFQYGPLDQG
                     WWPDGLYTAPTDEALKYDIVRTKELGFNMIRKHVKVEPARWYTHCDELGILVWQDMPNG
                     GRGPGWQSRSFFKGSEDQRSERSENIYKKEWKGIMDHLYSYPSIVVWVPFNEAWGQFNT
                     VEITEWTKNYDPSRLVNSASGGNHFHTGDILDLHNYPDPNLYLYDAERVTVLGEYGGIG
                     LPLKKHLWKTDNNWGYVQFKNSDEVTDEYVKFAMKLKNLVKSGFSAAVYTQTTDVEGEV
                     NGLMTYDRKVDKVDVARVRKANQEVIKTLD"
     gene            complement(8781..9956)
                     /locus_tag="GRFL_0301"
     CDS             complement(8781..9956)
                     /locus_tag="GRFL_0301"
                     /codon_start=1
                     /transl_table=11
                     /product="GH5|GH5_13"
                     /protein_id="APU67025.1"
                     /translation="MRIITLITLVSLCFYSCKNAEGSTDKKDLAEAEATDSTNIAEVWT
                     KQQANDWYDKQPWLVGANFNPSTAINQLEMWQEKTFDTATIDKELGWAEEIGMNTMRVY
                     LHDLLHKEDAEGLYDRMDQYLAIADNHGIKTLFVLFDSCWDPFPKTGKQREPQPHVHNS
                     GWVQSPGQEALQDSTQYGRLKEYVIGTVGKFKNDDRILGWDVWNEPDNMTGPSYEDVEI
                     PNKKELVYNLLDQAFDWARSANPSQPLTSGLWTGDWSDHEKMEKMHKMQIAKSDVISFH
                     NYNTPQDFEKKINQLKRYGKPMLCTEYMARPNGSTFQGFLPVARKYNIAMYNWGLVDGK
                     SQTKYPWDTWTKKYTSEPDVWFHEVFQKDGKPYKKEETDLITQMTAEANAK"
     gene            complement(10103..10795)
                     /locus_tag="GRFL_0302"
     CDS             complement(10103..10795)
                     /locus_tag="GRFL_0302"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="APU67026.1"
                     /translation="MNTKYYILFLGLMLSLVSCEIDNYDEPQASFTGALTYQGDTIRVA
                     QNDVRFQLWQSGFGKEGPLDVHVSQDGSFSSLLFNGDYRLKFIEGQGPFKALSQGTSQG
                     DTIYLNLSGDTNMELEVMPYYMIRNPEMNLAGASVNASIDLQQVITGEDARDIEHVTLY
                     LNTTRFVSANGDMHVARADADLGDMSNLNMKVDIPGDVVQDYIFARVGVKISGVEDMIY
                     SPLQKLNF"
     gene            complement(10815..12710)
                     /locus_tag="GRFL_0303"
     CDS             complement(10815..12710)
                     /locus_tag="GRFL_0303"
                     /codon_start=1
                     /transl_table=11
                     /product="putative outer membrane protein"
                     /protein_id="APU67027.1"
                     /translation="MKMKKHISILMVMAVLAGSCTSDSEFLDREPTNILTQDQIFSDSE
                     LPLSILADFYNRYYDFGRIEDWPSVADFNVAFWSEAGRYGHFQNSDRGYGDWGNWDYGY
                     IREINLFMERLQASEELAESDKTRFLAEARFLRASYYFTLVKRMGGVPLILESEEYDFS
                     GDPSYLQHPRETEAAIYDFVINEAEAVKDILPASADVKSRASKGAALAMEARAALYAGS
                     IARYGQNTPSVSLPGGEVGIPASRAEAYYQTALDAAQAIIDGQAGNYSLYMKKPDLSEN
                     FASLFYDKSNNPEAIFVEDYMLQSGKVHGFTISNQPRYGAEEEEGGRINPSLNLVEAFE
                     TLEGECEPLPVKDNSGNYLKYENPEDIFEGRDARLKGTVIVPGSNFKGRQVDIWAGVLL
                     PDGTIISGDSRGQQKEFPGSNGQEQVVGFDGPIDGLEFTAQTGFYIRKYQDPKAGSGQR
                     GVQSDVWFIRYRYAEVLMNAAEAAFELGQPELSAKYMNEVRSRAGITTELTADDISFER
                     IAHERFVEFAFEGLYFFDLKRWRIAHIVMDGVPMTEEEVTSDVCDVTDRLTQPWGLWPY
                     KISDPGSSDDGQWTYRVMKPSRVTGADRFRLGNYYSEIGQDIINNNPLIVRNPNQ"
     gene            complement(12717..15857)
                     /locus_tag="GRFL_0304"
     CDS             complement(12717..15857)
                     /locus_tag="GRFL_0304"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="APU67028.1"
                     /translation="METLLLRFKRPISGSILICCLLLNLVAQAQDKQEISGTVTSQGED
                     PEPLPFINVMVKGTTRGTTTDFDGNYSIEAAPDATIVFSYLGYKTLEVPVNSQTEISVQ
                     LEEDVAALDEVVVTGYGSQEKKSITGAISTVTSNDLERVHGGSTVSSGLAGKIPGVSFR
                     MADGRPGASAAIQVRNMGTPLYVIDGIQQDEGQFNNLAPNDIESITVLKDASAAIYGVR
                     AANGVVVVTTKKGKKNSKSTVNIDSYVGWQNWSRFPDPVNDSYTYQQLVAESQVNEFGS
                     TNITPEELEKYRVGEEMGYQSFNWKDFIIKKNAPLVNLNVNASGGSENINYYLSATNLK
                     QESVLGDEFRFERSNIQSNVNANITDRLKVGVQINGRIEERLNPGIPGADDYWLPRFAI
                     LRNRPYERPYANDNPEYLNDIGHNETNWGLHNFEIGGYKRDTWRVLQTNFTAQYDLPFV
                     EGLNLKGMYSYYIADHLMNGHEYTYEAYTYDSQTDTYNATGGSTNPWREREQNKVLRNI
                     YQGQLQYKRDFGDHGLDAMFIVERQEARFLRNWVHSVPKTNVLPLIYFSDVDTYDDSES
                     EEARIGYIAQVSYNYKDKYYLSFSGRRDASWKFAPDQRVGYFPSVSGGWRITEEQFMKD
                     WLGDSTILDNLKIRGSYGVLGDDNIGIGAFDYLTGYNYNTGIAILDGQPVIASRDKGQP
                     ITNITWFKSKITDIGLDAYLFDGKLSATFDYFYRLRTGLRGRKYDILLPSELGYGLPDE
                     NVNSDAQFGIEGALSYNNTFGDVTFNVSGNASLTRSKFISSYKPRFNNSWDRYRWSGED
                     RYNTIFWGYTALGQFQSQEEIDNYPVDIDGRGNETLLPGDIIYKDVNGDGRIDGYDERP
                     IGYNNYNPLVNFGLSFYVAYKNFDVSADFSGASGYSWNQEWETRIPFQNGGALNEIFLD
                     RWHRADVFDPNSEWIPGKYPALRYNNRGHSNYNKNSTFWLHNVTYLRARTLELGYSIGD
                     SILEKLKIQKARFYVNAYNLFSIDNLQQYGIDPEVNDTNGLQYPQNKFVNVGINLSI"
ORIGIN
        1 tcattccgtt tgtttccatt cgtttgcatt ttcaccttcg ccaataaccg ggaaactgct
       61 tattcttaat ctggccgctc ccatcgggat cagctctacg gtttcagttt cttcggaaga
      121 tttcacaggg ctctcctgta attctccggc caatccatat tgatcgatcg tccattgcgg
      181 aatttgcttt gctttggctt tcagtacaat tggtgtagtt tccggcgtaa atggaaaatc
      241 gttttccggc cagtctcttt cttcaattgt aaaagagttt gttacgtcgt cttcaagcag
      301 caaaccataa ttccagtcgg ttgttggatg aatttcataa gatggccatt ttttagtatc
      361 cgcaccttcc tgccatttgg aatcccaaac agccgtttca tcgctttgtt tttttgtgta
      421 ttcctcccca attttcagag aaaagcttag cggaccataa tttacactaa cgctattatg
      481 gttattctcc cactttttca tttccagttt cttcggaagg tccaaagtca cgacatctcc
      541 attttgccag tttcgatcaa tccttacata ttttcctgct tcgggagctg tttcagtttt
      601 tgtaccgttg atacttacag aagcttcctg agcccaagat ggaatcctga aatacaatgg
      661 gaaagcagtc cctttttccg aagtggaaat actgaatttt atcgcctgct caaaaggata
      721 gtgagtttcc gtagaaattt caacctcctg cccgtctgca actttcgccg aaacagtgga
      781 aggtccgtaa ataacagctg ccagaccgtt atctggcgtt gccatccaaa gattttctac
      841 catatatggc cagccctgac catgattatg ctggcagcaa cgggaactga aagggttcat
      901 cattaagaaa ggaccatcgt tcataattcc cggacgatga ttttgatcgt cattcaacac
      961 catattcgga ctggtgatat agcgcaagga tttcatatct ggcatcatgg tagccgggaa
     1021 cgtattaaag gcaacctctt ctgtgtgatc tgcccagaaa gtatcgcctg ttatgcgcag
     1081 taagctctcg ttggagttca tttgctccac cacaccacag gtttcaattc cctggcgtgg
     1141 atcgtcataa ccaggacggg cattttcatc ggcgccatac attccgccgg gcacctgccc
     1201 aaaatgttct cttacgatct tgaaattatc ataggttgcc ttcagatcct tctcatcatg
     1261 gcttagctgg taatactgcg ccggctcacg aaaagcctgt gccacattca cattatgcca
     1321 gtcgatcaaa ttggaatacc atccagggta atccatatta tcccgcgttt ccttccagtt
     1381 atggatgtca tctatggaat tatctctttt agaccaatta gacgtattac ggtggatctt
     1441 ctccatcaat tccagcagga attcatcacc ggtacgattg tacaaccaga caacgctatg
     1501 caggttgtca ccaccacgaa ccttctgcca gtaatggctt ccagataaaa attcttcgtc
     1561 tggaacatta ttctgaaatt taaaataatt ggtcatcagg tctatcaccc gatcgtctcc
     1621 ggagtattcg taatacgact gaaggcaata taacatgatc atgtttgccc aaaaatcctg
     1681 tgtgccatcc tcacccaaac gctcgggacc aaaatttcca tcctcacgct gactcgcaat
     1741 ggcagcttcg atccatttct cggtttcggt gatcattttt tcatcaccca gaatataggc
     1801 ggtgttccca tagcccttca gccaatatgg aacctcctcc cagccccaga aacctttacc
     1861 atcctcggcc agccatgcat tatctttttc ctgtaaccac gcactgatct ctccgagatt
     1921 ccccattaaa ccattggcct gccttttcat catttccttt aaccagccat ttggtttgac
     1981 tgctccaaca ggaagtttga taagcacgct tgatgcgagt ggtttttgat tagaaacgta
     2041 attggtattc gtcgcgctgg tatcaggaat ttttgcaata cttagtgttt gttcctgttt
     2101 cttttcagaa ttacaggaaa gacagactat cgcgataaaa atgcttatcc agctggttaa
     2161 ttttgacatc atctattttt taagaattta ctcccactcg tcacgtagca gcttaataaa
     2221 gtaccctccg atcacacttc tagcctggaa accacgctga tcaccactgg tcgtaaaatg
     2281 ccagtcactc ataggaactc tattcttggt ttcggtcgca aattcatata ccggatcggc
     2341 aaatttttca aaagtcgcct ggtcatcggc catggtggcc gtccatagaa tccagtcaga
     2401 ttttgtgtaa tctgcacggt tatctaatgg cagaccgtac tggttttgct tggtgtcgta
     2461 gaatttcaac tctgtatcaa aaacttcctg agggaagatt tccaggtcta aaactttatc
     2521 ccaaaccaag ttgtatttct ggctccaggt atttttatca ttataagtca acgcatagtg
     2581 atcaccggca tctgccagtt ccatccattc tttcgccatt tctttagctt tctgcgtgta
     2641 ttcttcagcc acatcgtctt ttccaagctg cttggcaaca taaccgtaac cacctacacc
     2701 tacaattgct ttagcagcga ggtttgcatt tcttgccagg tgaccggaaa aatcatcggt
     2761 agataactga ttcgcaggat ccagtccggc ttcagttaag taattagccc atacggttaa
     2821 cgattcccag tgctttttgg cataatcggc attcccttca gctttagtga ttgctgctgc
     2881 caataccacc atattacccg attcttctac cggcatatct tcaccataag tttgcccggt
     2941 tgcgattgga taagttccaa ggtcatgtgc cgggaaaggc tttttccatt ttccgctttc
     3001 agaataatag aaaatcccgt tcatcattcc ctttaaaaga tctggattgt ataacaaata
     3061 catcggtgcc gatggatagg taatatctac cgtattgatc gacccgttac tgaagttttc
     3121 cttggacatg aacaagatct caccatcagg acttttggtc agtttatgtg cagcaatact
     3181 ttgtctatag gcaatttcaa gtatcttggc atattcctct ccgcctgctt ccacgccgtc
     3241 ttcatgcagt tcctcattga agtcttcaca tttttcaatt acttcatcat attcagcata
     3301 ggctttttcc agttcgttct ggatcgaatt ggtcccatct aaattccacc agggtcttaa
     3361 ttggtttccg aaataattga tagaataaat atcgtcgtaa cccagcatga tcaaatgttc
     3421 cttttcttcg gaaagttttt cagctgggaa aatggtattc agcatcaggc tctttccatc
     3481 tcttacggaa gcgctcatgg aattgctcat gaattctttg gaagcatcag ttcctttggt
     3541 gatattttgt tttgcgttgg catctttcgc cacagccaca tacatgtaac cccagtcaat
     3601 tctcaggtta tcacctttct tttccagaat tggctgttct ttggttcccg ctttcaaaaa
     3661 attcaatgct gaagtcgagc cagattctgt ttccattaac tggcttcttt cattagaagc
     3721 gatcagggaa gaagctccaa aataaacctg aacatcatgg atctggccat catttggttt
     3781 tgtctttaca gaaatatagg aaaccggtct ggccagtaga tccaggtcat ccattaaaag
     3841 cggggatgta aacgtcaaat ttacatctac gccaccggca gtaagcatat attttgtctg
     3901 agtcgcggtg aaatccaggc cagtctgctc ggcaagttct acctgagttt taggcttttt
     3961 tacttcaaca attccggcat ccagaaatct tccgccggct gtatttttaa tatggatagc
     4021 caggatatta tccttcttct taagtttcga ttttacttcc tcagaaattg ggaaataatc
     4081 gtactggtca ttccagccag tcttttccag gattttctcc ccgtttagat aaaccacaat
     4141 attatcatca tgatgaagct ttaaaaacac gtcatcaaaa ttgctgtttt tcaggtcgaa
     4201 ctttcggcga acccaaagat cttcgctttc ccatttcgtt acataatcat gctcatctcc
     4261 aaatggcgct ttggtacttt tccagttaga atcatcaaaa tcggccttct gccagcctga
     4321 ttttggttca tcttccgtag ccattaccgt atactctccg gcatctgctg cggaaagtac
     4381 atctacataa tttttaggtt cattccccag gaaacgataa acttttccgt cgacctttat
     4441 caaccccacg aggctctgat cggtacccgt ccagtgaacg gtagactgct cattgatctt
     4501 atcaccaaaa gaccagatac tgaagttcgg gtcgtgagta actaagggat aagccggcgc
     4561 ctgcctttgc tgggccgtga gtattcccga aaatatgatc gcggttatga aaactaattt
     4621 ctttatcatg ggtgttttta ttgattcgtg gttgaaaatt tatattctga aacggtgtga
     4681 taggtgtcac ctggctccag tgtgatctgc ggaaattcag gttgattcgg tgcgtctgga
     4741 aagtgttgtg tttccatggc aaaagccgta cgatattcat ctttagcacc agatttgaac
     4801 gtattcttcg actgcatgaa atttccgcta tagaactgaa gtcccggttc ctgcgtgaaa
     4861 atatccatca cgattccaga ttgatcgcct gtaacggtcg cagctttatt cagatcaccg
     4921 gtctgagctt cggtaagcac gaaattgtga tcgtaacctc ctccattttt aagttgttca
     4981 ttatccgttt caattcgctc accaatggtg tgcggttgtg taaaatcgaa aggagtacct
     5041 gccacttcct ttaattctcc ggaagggatc aaaccaccat caaccggcgt gaatttatca
     5101 gcctcaatct gcacttcatg ctgaagaatg cttccgcttc cctcaccatt taaattgaaa
     5161 aaggcgtgat tggtcagatt taccggagtg gctttatctg tcgttgcttc atattccatc
     5221 atcacggagc gatctgaggt caatgagtaa gtaaccgtga catcgaggtt tcccggaaag
     5281 ccctcttcca tatctggcga tttataatga agcaccaggg tgctgtcatt tcgcttttca
     5341 ccatcccaaa ccacatactg gaaacctttt tttccgccgt gtaaagtatt cccgttattg
     5401 ttctgcggaa ttgtatattc ttccccattc aatttgaatt taccttttgc gatacgattt
     5461 cccacacgac ctatggtagc tccaaaatag ggttcagtgg cattggcgaa accttctgca
     5521 ctgttcatcc ctacaacaac atctgtcatt tttccgtttt tatccggaac ccaaagacca
     5581 acgagacggc ctccataatt ggtaaaagct gctttaatac tgtcattttt cagccagtac
     5641 aaactcactt ttttatcgtc gataagcgta tcaaaatctt ttgcctgcag ttccatttct
     5701 gaaacggcgg tagagtctac tttggtagat tcggctgtat cctccttctt gtcatcttta
     5761 caggaagtaa aggaaatcat ggtcgctagc gctaatgccg acaggtattt aattttcatt
     5821 ttcagtattt tttatttcgc tggtgccgga aggcaattcc agttcggttc ccggggctac
     5881 cggagttcca aaatctggag taccatctgc attccatgtt attttttgca ttcttggaga
     5941 gcgatttcca ccacaaccgt ctcccgaatt cggattggcg tggtacaaga tccagtcttc
     6001 tgttccatct ggtgatttaa aaaacgagtt atgaccaggg ccatagacgc cattttcagg
     6061 agattgtgaa aaataaggtt caggatcttt ttcccagttt tcggcatcca gaagatcatc
     6121 gccagttaac ttaagcaatc ccaaactgta attatcggtc cagcaaccgc tggccgaaaa
     6181 tacaatgaac aagttgtctc catgttccaa aaactgcgga ccttcattta ccaaaacttc
     6241 cgggccattt ccataatttc gttcccaatc aaaagtcgga gaagaaattt tgattctttt
     6301 tccttcgatc gtataggggt ttgacatctt cgcgatgaaa atatcctggc gaccattctc
     6361 atcaccttcc caacccgacc agatcatgta aagctgatcc tgaaattcat agatatcggc
     6421 atcaatcgcc catttgtctg atggtgcagc gacttttccc ttgaattccc attctccggt
     6481 cataggatct tccgaagaat tttcgagtac atacatacgg tgattctgat tgtcgccatc
     6541 atcagccgcg aaatacatat accacttccc ctccaggaac tgaacctgag gtgcccagat
     6601 atttcgggaa tacatggtat tttctggcgg tgtccagatc actttcattt cggcattacc
     6661 aatttctgaa aggtcttttg ttttccagat cgccagcttg ttgcctaacg tattggtgta
     6721 ataataatag ccgtctttat accagctgta cggatcggct cctgaagatt tcagcggatt
     6781 cgtaaaagtg gttttttctg aaactttcgg tgtattctca ttaacagcaa catcgctgtt
     6841 gccatcctta caggatacaa cagcgagtgc ccctaacaaa acaattttcg ttacaattcc
     6901 tttactcatc tttcaactta gtccaatgtt ttaataactt cctggttcgc tttacgaact
     6961 cttgccacat caactttatc aactttacgg tcgtaagtca tcaatccgtt tacctcacct
     7021 tctacatctg tggtttgtgt gtagacagct gcggaaaatc cagatttcac gaggttcttt
     7081 aatttcatgg cgaactttac gtactcatcg gttacctcat cagaattttt aaactgaaca
     7141 tatccccagt tgttatcggt tttccataaa tgctttttca gcggaaggcc aattcctcca
     7201 tattcaccca gaacagtaac cctttccgca tcgtacaaat aaaggttagg atctggataa
     7261 ttgtgaagat ccaggatatc gccggtatga aagtgatttc caccactggc cgaattcact
     7321 aatcttgaag gatcgtaatt tttagtccac tcggtaattt caaccgtatt gaactgtccc
     7381 catgcttcat tgaaaggaac ccaaaccacg atactcgggt aggaatacag gtgatccatg
     7441 atacctttcc attctttctt ataaatattt tctgaacgct cgcttctctg atcttcgctg
     7501 cctttgaaga aacttcttga ctgccatccc ggaccgcggc ctccattcgg catatcctgc
     7561 caaacaagaa ttcccaattc gtcacaatgc gtgtaccatc ttgcaggctc aaccttcaca
     7621 tgttttctga tcatgttaaa gcctaattct ttcgttctta caatgtcata ttttaatgct
     7681 tcatctgttg gcgcggtgta tagaccatca ggccaccatc cctgatctaa cggaccatac
     7741 tggaaaatat cttcgttatt cagctgcatt ctgataatgc cattttcatc tcttttactg
     7801 gagatcttac gcattgcgaa ataactcttc acctcatcct gaacctctcc attgctgata
     7861 agcttcacct cagttcggta taaaaatggg gaatctggcg accataattt tggatcattt
     7921 acatgaatgc tgaattcctg gtctaaagat gatttggtag tgctgatctc tttattacca
     7981 tcgtaaacaa ccacttcgat catatctccg taagctgaat tgcttccttc ggcagaaacc
     8041 tttacggtat tattatcgat atctggagta aatcttaaat tctgaaggct tttttcagca
     8101 acaggttcca gccagactgt ttgccagatt ccggttaccg gagtatacca gatcccttca
     8161 ggattcttaa cctgcttacc tctaggctgc ggcccttcat cactgggatc ccaaaccttt
     8221 acagttaatt tttgttcccc atccttattc aaaaatggcg tgatatcaaa agaaaacggc
     8281 tcgtaacctc cggaatgcga acctaatttg atgtcgttga tccagacatc tgcctgccag
     8341 tctacggcgt caaaatttaa tttgatcttt ttaccattcc agtctccagg aaccgtaaaa
     8401 gtggtgttat accataattc attttcagca ccgacagttt tcataacccc ggaaagactt
     8461 gattccactg caaatggcac caaaatatct ccgtcaaaat tctgtggttt cgctttgcct
     8521 ttttgctgga tactgtattc ccagagtccg ttaaggtttt tccagtccgg tctttccatg
     8581 atcggtcttg gatattcatt taaaggctta tcggggttta cctcatctgc ccatttactt
     8641 ttgatcttgt cacccgccgg cttccactgg gcttgcatgg taacctggct tagcaggact
     8701 aagcacagga tcgttatatt tttaattata gaattcattg tagtagtttc agttctgttt
     8761 aataattctt tttcggattt ttattttgca ttcgcttcag cggtcatctg tgtgatcaga
     8821 tcggtttcct ctttcttata aggcttacca tctttctgaa agacctcgtg gaaccagaca
     8881 tctggttcag aagtgtattt tttagtccaa gtatcccatg ggtatttggt ttgacttttt
     8941 ccgtctacaa gaccccagtt atacatggct atgttatatt ttctggcaac cggtaagaat
     9001 ccctggaaag tacttccatt gggacgcgcc atatattcgg tacaaagcat tggtttacca
     9061 tagcgcttta actgattgat ctttttctca aaatcttgag gagtattata attatggaaa
     9121 gaaataacat cagattttgc aatctgcatc ttgtgcattt tctccatttt ttcgtgatca
     9181 ctccaatctc ccgtccaaag gccagaagtt aaaggctggc ttggatttgc tgatctagcc
     9241 caatcgaatg cctggtccag aagattataa accaattcct ttttattcgg aatttcaaca
     9301 tcttcataag aaggacctgt catattatct ggttcattcc aaacatccca gcccagaatt
     9361 cggtcatcat ttttaaattt tcccacagta ccgatcacgt attcttttaa tctgccgtac
     9421 tgtgtagaat cctgtaatgc ttcctgcccc gggctttgga cccaaccaga attatgaaca
     9481 tgaggctgcg gctctctttg tttccctgtt ttagggaacg gatcccagca ggaatcgaat
     9541 aatacaaata gtgttttaat accatgatta tcggcaatgg caagatactg gtccattcgg
     9601 tcatacaatc cttcagcatc ttccttatgt aaaagatcgt gaaggtaaac cctcatggta
     9661 ttcatcccga tctcttctgc ccagccaagt tctttatcaa tagtagcagt atcaaaggtc
     9721 ttttcctgcc acatttcaag ctgattgatc gcggtactcg gattaaaatt agctcctacc
     9781 agccatggct gcttgtcata ccagtcattg gcctgctgct tggtccaaac ttcagcaata
     9841 ttagtagaat ctgtcgcttc agcttctgcc agatcttttt tatctgtgct cccttcagca
     9901 tttttacagg agtaaaaaca taggctaacg agcgtgataa gggtaataat tcgcataatt
     9961 ttcattgcaa tataattatt tccgccaaat aactaaatcg ttttaggcga ttctattgat
    10021 ttaaagttat ctctcccggt cattttcttt attctgaaaa atgaccggga ttgataacaa
    10081 ggctaactaa atcaacaggt gattagaaat ttaatttttg aagcggggaa tagatcatat
    10141 cttctactcc gctgatcttg actcctacac gggcaaaaat gtaatcctgg accacatcac
    10201 cagggatatc aaccttcata ttcaagttac tcatatcccc taggtcagca tcagccctgg
    10261 caacatgcat atctccattt gctgaaacga atcgagtcgt attcaggtaa agtgtgacgt
    10321 gttcaatatc ccgggcatct tccccggtta ttacctgttg taggtcgatg ctggcgttta
    10381 ccgaagcgcc tgcaagattc atttctggat ttctgatcat gtaatagggc atcacttcca
    10441 gttccatatt ggtatcccct gataggttta ggtaaatagt atcaccctgg cttgttccct
    10501 gcgaaagtgc cttaaagggt ccctgacctt caataaattt taggcggtaa tcaccattaa
    10561 aaagcaagga gctgaaagat ccatcctggg aaacatgcac gtctaaggga ccttcttttc
    10621 cgaagccgga ttgccacaac tggaagcgca catcattctg agctaccctt atagtatctc
    10681 cctggtaggt aagggcaccc gtaaagctgg cctgaggttc gtcgtaattg tctatctcgc
    10741 atgatactaa agagagcatc aggccgagaa aaagtatata atatttagta ttcataatct
    10801 tcaaatttag agtcttactg gttaggattt ctcacgatca atgggttatt attgatgata
    10861 tcctgaccaa tttctgaata gtaatttcct aatctgaaac gatcggcacc ggtcaccctg
    10921 ctaggtttca tcaccctata ggtccactgt ccatcatccg aacttcccgg gtcactgatc
    10981 ttgtaaggcc ataatcccca tggctgtgtc aggcggtctg tcacgtcaca aacatctgaa
    11041 gtaacttcct cttcggtcat cggcacaccg tccatcacga tatgagcgat cctccatctt
    11101 ttcaggtcga agaaatacaa accttcaaaa gcaaattcca cgaaacgttc atgtgctatt
    11161 ctttcgaatg aaatatcatc agccgttaat tcggttgtga ttcccgctct ggaccgaact
    11221 tcgttcatgt attttgctga aagctccggt tggcccaact caaaagctgc ttccgcggca
    11281 ttcatcagaa cctctgcata acggtaacgg atgaaccata catcgctctg tacgcctcgc
    11341 tgaccagaac ctgcttttgg gtcctgatat ttccggatat aaaaaccggt ttgagcggta
    11401 aattccagac catcaatagg cccatcaaat ccaacgacct gttcctggcc gttcgatcct
    11461 gggaattctt tttgttgccc tctcgaatca ccagaaatga tcgttccgtc aggcagtaaa
    11521 acgcctgccc agatatcaac ctgtcttccc ttaaaatttg aaccgggaac gatcactgta
    11581 cctttaagtc gggcatctct tccttcgaaa atatcttcag gattttcata tttcaggtaa
    11641 tttccggaat tgtctttaac cggtaatggt tcgcattccc cttccagagt ttcaaaagcc
    11701 tccacaaggt tcaaagaagg gttgatcctc ccgccttcct cttcttccgc gccgtaacga
    11761 ggctggttgg aaatggtgaa accgtgaacc ttcccgctct gaagcatgta atcttccaca
    11821 aatatcgctt caggattatt cgatttatca taaaacaggc tggcgaaatt ttctgaaaga
    11881 tcaggtttct tcatgtacaa tgaataatta cccgcctgcc cgtcaataat tgcctgagct
    11941 gcgtcaagtg ctgtttggta ataagcctct gcccgcgaag ctggaatccc aacttctcca
    12001 cccggaagcg aaaccgatgg cgtgttctgg ccatatcgcg caatagaacc ggcatataaa
    12061 gccgctcgag cttccatggc cagtgctgct cctttggagg cacgcgattt cacatcggcg
    12121 ctggccggca gaatatcttt taccgcttcc gcttcgttga ttacaaaatc atagatcgcc
    12181 gcttctgttt ctcgcggatg ctgaagataa ctcgggtcgc cgctaaaatc gtactcttca
    12241 ctttcaagaa taagtggtac tcctcccatt ctttttacca atgtaaagta ataggaagct
    12301 cgaaggaatc gtgcttcggc caaaaacctg gttttatcac tttcagcaag ttcttcggaa
    12361 gcttgcaggc gttccatgaa caggttaatc tcacggatgt agccataatc ccagttaccc
    12421 cagtctccat atccgcgatc agaattctga aaatgcccgt atcttccggc ttctgaccag
    12481 aatgccacat tgaaatcagc tacactcggc cagtcttcga ttcttccgaa gtcatagtac
    12541 ctgttataaa aatcggccag gatagacagc ggtaactcag aatcactgaa gatctggtcc
    12601 tgtgttagaa tgttggtggg ctcccggtct aaaaactcac tgtctgaagt acagctacca
    12661 gccagcaccg ccatcaccat taaaatcgat atatgttttt tcattttcat cagtttttaa
    12721 atggaaagat tgattcccac atttacgaat ttgttctgcg ggtattggag cccgttcgta
    12781 tcattcactt ccggatcaat accatactgc tgcagattat caatcgagaa aaggttatag
    12841 gcgttcacgt aaaaacgggc tttctgaatc ttcagttttt ccaggataga gtctcctatt
    12901 gaatatccca attccagtgt tctagcgcgc aggtatgtca cattgtgcag ccagaatgtg
    12961 gaatttttat tatagttact gtgcccgcgg ttattgtacc tcagggccgg gtatttcccc
    13021 gggatccatt cgctgttcgg atcaaagaca tcagcccggt gccagcggtc caggaaaatt
    13081 tcgttcagcg cccctccatt ttgaaatgga attctggttt cccattcctg gttccaggaa
    13141 tatcctgaag ctcctgaaaa atctgcagag acatcaaaat tcttataggc cacataaaaa
    13201 ctaagcccaa aatttaccag tgggttataa ttattatagc caattggtct ttcatcatat
    13261 ccatcaatac gcccatctcc atttacatct ttatagataa tatctcccgg tagcagagtt
    13321 tcatttccac gaccatctat atccaccgga taattatcga tctcctcctg agactgaaat
    13381 tggccaagag cggtatatcc ccagaatatc gtattgtagc gatcttctcc agaccagcga
    13441 tagcggtccc acgaattatt aaagcgaggt ttataagagg agataaactt gctacgagtg
    13501 agggaggcat tcccggaaac attgaaagta acatctccaa aagtgttatt atagctcaaa
    13561 gcgccttcga taccgaattg agcgtcagaa ttcacatttt catccgggag tccgtagcct
    13621 agttcgcttg gaagaagaat gtcatatttt cttccgcgca ggccggttct cagcctatag
    13681 aagtaatcga aagttgctga aagtttaccg tcgaataaat aagcatctaa accaatatct
    13741 gtaatcttac tcttgaacca ggtgatattg gtaattggct ggcctttatc acgactagcg
    13801 atcactggtt gaccgtctaa aatagctatc ccggtattgt agttataacc ggtgaggtaa
    13861 tcaaaagcgc caatcccgat attatcatct cccagcaccc cgtaggaacc gcgaatcttc
    13921 aggttatcga gaatagttga atcaccgagc cagtctttca tgaactgctc ttctgtaatt
    13981 ctccatccac cggaaaccga tgggaagtat cctactcgct ggtctggcgc aaatttccag
    14041 gatgcatcac gccttccgga gaaactgagg tagtacttat ctttataatt ataagacacc
    14101 tgcgcgatat agcctattcg ggcttcctca ctttcactat catcatatgt atccacatct
    14161 gaaaaataga tcagcggaag cacgtttgtt tttggtacgg aatgcaccca gtttcttaag
    14221 aatctagctt cctgtctttc cacgatgaac atggcatcca ggccatggtc accaaaatct
    14281 cttttatact gaagctgtcc ctggtagatg tttctcaaca ccttgttttg ttctctttcc
    14341 ctccagggat ttgtactccc tccagtggca ttatatgtat cggtttgaga atcataggta
    14401 taagcctcat acgtgtactc atgcccattc atcaggtgat ctgcgatgta gtaagaatac
    14461 atgcctttaa ggttaaggcc ttctacaaaa ggcagatcat attgagctgt aaagttcgtc
    14521 tgtaaaaccc tccaggtatc ccttttataa ccgccaattt caaaattgtg caatccccag
    14581 ttagtttcat tgtgaccaat gtcattgaga tattcaggat tgtcattggc ataaggtcgt
    14641 tcgtaaggac gattacggag aatcgcaaaa cgaggcagcc agtaatcatc agctcctgga
    14701 atacctggat taagacgctc ttcgatcctt ccgttgattt gtacacccac cttcagccta
    14761 tcagtaatgt tggcattcac attactctga atattacttc tttcaaagcg gaattcatct
    14821 ccaagaactg attcttgctt cagattagtg gcagagaggt aataattgat attttcagaa
    14881 cctcctgatg cattcacatt taaattaacc aagggtgcat ttttcttgat aatgaaatct
    14941 ttccagttaa aagactggta acccatttcc tcaccaactc ggtatttctc caactcttcc
    15001 ggcgtgatat tggtgcttcc aaactcgttg acctgtgatt ccgctaccaa ttgctggtaa
    15061 gtataagaat cgttcacagg gtctggaaaa cgcgaccagt tttgccatcc tacgtaagaa
    15121 tctatattta cggtgctttt ggagtttttc tttcctttct tcgtagtgac taccaccacc
    15181 ccgttcgcag cacgcacccc gtagatcgct gctgaggcat cctttaaaac cgtaatactt
    15241 tcgatgtcat ttggtgcaag gttgttgaac tgaccttcat cctgctgaat accatcgatc
    15301 acgtaaagtg gtgttcccat attacgaact tgtatggctg cactggcacc tggtctaccg
    15361 tcggccattc tgaagcttac gccaggaatc tttcccgcga gccccgaact tactgtagac
    15421 cctccatgca cacgctccag gtcattgctg gtaacggtgg aaatggctcc tgtgatcgat
    15481 ttcttttcct gtgaaccgta tccggtcacc accacttcat ccagtgcggc gacgtcttct
    15541 tccagttgaa cagaaatttc agtttgcgaa ttcaccggga cttctaaagt tttataacct
    15601 aggtagctaa acacaatcgt agcatcggga gcggcttcaa tactataatt tccatcgaaa
    15661 tcagttgtgg taccgcgagt agttcctttt accattacat tgataaatgg tagcggttca
    15721 ggatcttcgc cctgggaggt aaccgtaccg ctgatctcct gtttatcttg tgcctgcgcc
    15781 acaagattca acaagagaca acaaatcagg atacttcctg aaattggtct tttaaatctg
    15841 agtaataatg tttccat
//
