LOCUS       FP476056                4377 bp    DNA     linear   BCT 27-MAR-2015
DEFINITION  Zobellia galactanivorans strain DsiJT chromosome, complete genome.
ACCESSION   FP476056
VERSION     FP476056.1
DBLINK      BioProject: PRJEB8976
            BioSample: SAMEA3315864
KEYWORDS    .
SOURCE      Zobellia galactanivorans
  ORGANISM  Zobellia galactanivorans
            Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Zobellia.
REFERENCE   1
  AUTHORS   Thomas,F., Barbeyron,T., Tonon,T., Genicot,S., Czjzek,M. and
            Michel,G.
  TITLE     Characterization of the first alginolytic operons in a marine
            bacterium: from their emergence in marine Flavobacteriia to their
            independent transfers to marine Proteobacteria and human gut
            Bacteroides
  JOURNAL   Environ. Microbiol. 14 (9), 2379-2394 (2012)
   PUBMED   22513138
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Genoscope -,C.E.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Genoscope - Centre National de Sequencage :
            BP 191 91006 EVRY cedex - FRANCE (E-mail : seqref@genoscope.cns.fr -
            Web : www.genoscope.cns.fr)
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Zobellia galactanivorans"
                     /mol_type="genomic DNA"
                     /strain="DsiJT"
                     /db_xref="taxon:63186"
     gene            1..1749
                     /locus_tag="ZOBELLIA_4201"
     CDS             1..1749
                     /locus_tag="ZOBELLIA_4201"
                     /EC_number="3.2.1.-"
                     /function="Hydrolase activity, hydrolyzing O-glycosyl
                     compounds"
                     /note="Possible glycosyde hydrolase belonging to the family
                     9 of glycoside hydrolases (GH9); Signal peptide cleaved
                     between the residues 25 and 26; Localized in the
                     periplasmic space; Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="Glycosyde hydrolase, family GH9"
                     /protein_id="CAZ98336.1"
                     /db_xref="GOA:G0L321"
                     /db_xref="InterPro:IPR004197"
                     /db_xref="InterPro:IPR008928"
                     /db_xref="InterPro:IPR012341"
                     /db_xref="InterPro:IPR013783"
                     /db_xref="InterPro:IPR014756"
                     /db_xref="UniProtKB/TrEMBL:G0L321"
                     /translation="MPEYKLKMKYILSLLFLLISFFGFSQKPYDYTLLTYSQVGYDSHM
                     PKHAMIQNTDSTFLSADATFILRRASDHKKVLKGKIGKSTKKWNKFWWKVDFSDCKTAG
                     AYVLEIRDHNKPIFQSKKELPITIGKKRLWDETWYATALSHLHIRDSLSYQPEGGWKDC
                     GSPLQEVSSHIIMLNALCDLLELDKENLSQAHKSEIHEQLIVGANYVALCQDKAQELGF
                     KEGAVIHEARENLKVVTGNVAKTAMILAKISRLIRAENPSLANAYKARSLKSYQWISEY
                     GPVLHWDNTDNFAITHGAPYGMKRPPKEWMTRDLVMMMWASLELYKCGELEYKNKAVDY
                     ASKIIKRQVPKDRAEDGLYGHFYTYDSYDFTEKADIHCGAWNANYKAYNQGGHFPHYLI
                     PLIEMMDLWGDHPNAVSWKACVDSFAFNYFLPATHQNPFKILPAGYYKNEGLLNWSGWY
                     HGHNKIFGYAAGLAMEFYNLYHDDRFIEIAVGNLQWISGLHSGFHENTEDFSASMIVGI
                     GNRSKSDWDAMTGTITNGFEADGQFKLSKPKKETDLPTVFGDEGGIHHPAGWISGLTRL
                     YYYQKK"
     gene            2183..2533
                     /locus_tag="ZOBELLIA_4202"
     CDS             2183..2533
                     /locus_tag="ZOBELLIA_4202"
                     /note="Contains three transmembrane helices; Localized in
                     the cytoplasmic membrane; Hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical membrane protein"
                     /protein_id="CAZ98337.1"
                     /db_xref="UniProtKB/TrEMBL:G0L7W4"
                     /translation="MFIIEFFSIGRGFSMGNKFSDAFSHILLMATITAILGKLLIVLSL
                     VIKKTIAKNAIGIIGVLLLYLSFRYLAYPTLPHTDFHKWAFWSGLPFLLVSIFLFHEQY
                     TVLKASFKKKST"
     gene            complement(2758..4377)
                     /gene="agaA"
                     /locus_tag="ZOBELLIA_4203"
     CDS             complement(2758..4377)
                     /gene="agaA"
                     /locus_tag="ZOBELLIA_4203"
                     /EC_number="3.2.1.81"
                     /function="Hydrolyses the beta-1,4 linkages in agarose"
                     /note="The beta-agarase A is a modular protein that
                     hydrolyses the 1,4-beta-D-galactosidic linkages in agarose,
                     giving the tetramer and hexamer as the predominant
                     products; Contains a N-terminal catalytic module (20-295)
                     that belongs to the family 16 of glycoside hydrolases
                     (GH16) and two C-terminal domains (296-437 and 438-539) of
                     unknown fuction; Signal peptide cleaved between the
                     residues 19 and 20; Localized in the periplasmic space then
                     secreted in the extracellular medium; High confidence in
                     function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="GH16"
                     /protein_id="CAZ98338.1"
                     /db_xref="GOA:G0L322"
                     /db_xref="InterPro:IPR008979"
                     /db_xref="InterPro:IPR013320"
                     /db_xref="InterPro:IPR026444"
                     /db_xref="PDB:1O4Y"
                     /db_xref="PDB:1URX"
                     /db_xref="UniProtKB/Swiss-Prot:G0L322"
                     /translation="MKKNYLLLYFIFLLCGSIAAQDWNGIPVPANPGNGMTWQLQDNVS
                     DSFNYTSSEGNRPTAFTSKWKPSYINGWTGPGSTIFNAPQAWTNGSQLAIQAQPAGNGK
                     SYNGIITSKNKIQYPVYMEIKAKIMDQVLANAFWTLTDDETQEIDIMEGYGSDRGGTWF
                     AQRMHLSHHTFIRNPFTDYQPMGDATWYYNGGTPWRSAYHRYGCYWKDPFTLEYYIDGV
                     KVRTVTRAEIDPNNHLGGTGLNQATNIIIDCENQTDWRPAATQEELADDSKNIFWVDWI
                     RVYKPVAVSGGGNNGNDGATEFQYDLGTDTSAVWPGYTRVSNTTRAGNFGWANTNDIGS
                     RDRGASNGRNNINRDINFSSQTRFFTQDLSNGTYNVLITFGDTYARKNMNVAAEGQNKL
                     TNINTNAGQYVSRSFDVNVNDGKLDLRFSVGNGGDVWSITRIWIRKVTSNSANLLAAKG
                     LTLEDPVETTEFLYPNPAKTDDFVTVPNSEIGSSIIIYNSAGQVVKKVSVVSENQKISL
                     EGFAKGMYFINLNGQSTKLIVQ"
ORIGIN
        1 atgcccgaat acaaattgaa gatgaagtac atacttagcc tccttttttt actgatatcg
       61 tttttcggtt tttcccaaaa gccatacgat tacaccttat tgacctattc gcaggtagga
      121 tacgatagcc atatgcccaa acatgccatg attcaaaata ccgattctac atttttgagt
      181 gccgatgcca cttttatatt aagaagggcc tcagaccata aaaaggtgct taaaggtaag
      241 attgggaaat ctaccaaaaa gtggaacaaa ttctggtgga aagtcgattt ttcagattgt
      301 aaaacggccg gcgcctatgt tttggaaatt agggaccata acaaacctat attccaaagt
      361 aaaaaggaat tacccattac cataggcaaa aaacggctct gggacgaaac ttggtatgca
      421 accgcgctat cgcacttaca tatacgcgat agcctgagct atcaacctga agggggatgg
      481 aaagattgtg gcagtccctt acaagaggta agcagccata tcattatgtt aaacgcctta
      541 tgtgacctat tggaattgga caaagagaac ctatcccagg cgcacaaatc ggaaatacac
      601 gaacaattga tagtgggggc gaattacgtt gccctgtgtc aagacaaggc gcaagaactg
      661 ggcttcaagg aaggtgccgt cattcatgaa gcccgggaga atttaaaggt cgtgaccgga
      721 aatgtggcca aaacggccat gatattggcc aaaatatcgc gtttgatacg ggctgaaaac
      781 ccttcgcttg ccaatgcata taaagcccgc tccctaaaat catatcaatg gatttcagag
      841 tatggccccg tgcttcattg ggacaatacc gataatttcg ccatcaccca cggcgcgcct
      901 tacggcatga aaagaccgcc caaggaatgg atgaccaggg atttggttat gatgatgtgg
      961 gcctcgctcg aactatacaa gtgcggagaa ttggagtata aaaacaaagc cgttgactat
     1021 gcttcaaaaa tcataaaaag acaagtaccg aaagaccggg ccgaagatgg cctttacggt
     1081 catttctaca cctacgactc ttatgatttt accgaaaaag ccgatataca ctgcggtgca
     1141 tggaatgcca attataaggc ctataaccaa ggcggacact ttccccatta cttgattccg
     1201 ttaatagaga tgatggatct atggggcgat cacccgaatg cggtgagttg gaaagcctgt
     1261 gtcgatagtt tcgcttttaa ctactttctg ccggccaccc atcaaaatcc ttttaaaata
     1321 ctacccgccg gatactacaa aaacgaggga ttattgaact ggagtggatg gtaccacgga
     1381 cataataaaa ttttcggcta tgccgcaggc ctcgccatgg agttttataa cctatatcac
     1441 gacgataggt ttattgaaat cgccgttggt aatttacagt ggatatcggg acttcactca
     1501 ggcttccatg aaaatacgga agacttttcg gcaagtatga tcgttggaat aggaaaccgt
     1561 tccaaatcgg attgggatgc catgaccggc accataacca atggctttga ggccgatggt
     1621 cagttcaaac tgagcaaacc taaaaaagaa accgatctac ctacggtttt cggcgatgaa
     1681 ggcggaattc accaccctgc cggctggatt agcggactca ctcgtttgta ttattaccag
     1741 aaaaaataag tcctgaaata gttccgtatg cgatggcgtg gaaatcgaca gtgccgacac
     1801 accgacccaa ccggcacaag cctgctgttc gctccgcgta aataaaaatg ccttgcaata
     1861 aatttccgct gcggaaggct cagtattaga ttttgaaggg cgcgcccagg gtaggggatg
     1921 tcctttccag gccaacaaac cattcaaagg tgaggaattt cttatattag tcgctgtgag
     1981 taggaggcat tcatggggat atctaatcat tatcaaatat gtggtccaca ccgaatacca
     2041 agcgaatacc atatccaaca ccttaaacac cgataaaaag cctactctaa tttctcttta
     2101 accgcgtatg aaaaaactca aaatttggac catagcctta cattccttaa ttcttctaat
     2161 tcataaaagc cttatttcca taatgttcat aatcgagttt ttctctatag gcagggggtt
     2221 ttccatgggt aataagtttt cggatgcttt ttcccatatc ttactgatgg ctacaataac
     2281 cgctatttta ggcaagttac ttatcgtgtt aagtctcgta atcaaaaaga ccattgccaa
     2341 aaatgcaatt ggaattatag gtgtgctttt actctacctt tcatttagat acttggcata
     2401 tcccacactt cctcatacgg attttcataa atgggccttc tggtccgggc taccttttct
     2461 tctggtgtct atattcttat ttcacgaaca gtataccgta ctaaaagcaa gcttcaaaaa
     2521 gaaaagtacc taatttaccc agtaaacagg gcatacacca tgtgccgaca ttgcacaaaa
     2581 acgaagcggt ttacagcaag tacaagaagc cccaaaacac agatcatatt ctagttagaa
     2641 gaagctaaca ataccaccta aaaatttatc ttggttcctg aagtaactta aaaaacgaca
     2701 aaggcctttc tgtatcatca gagcgccttt gtcgtttaat tgaaattgtt attgtgttta
     2761 ttggacaata agttttgtac tctgaccatt caaattgata aagtacattc ctttagcaaa
     2821 tccttctagt gatattttct gattttcgga aaccacactt acttttttca ctacttgacc
     2881 tgcactatta tagatgatta tactacttcc aatttcacta ttgggaacag tcacaaaatc
     2941 atctgttttt gcggggttag gatataaaaa ttccgtagtt tccacaggat cttccaatgt
     3001 taatcctttt gccgctaaca aattagcgct gttgctcgta acttttctaa tccagattct
     3061 tgtaatggac cacacatccc cgccattacc aactgaaaat cgcaaatcta gttttccgtc
     3121 gttgacattt acgtcaaacg acctactaac atattgcccg gcattggtgt ttatgtttgt
     3181 taatttattt tgcccttcgg ccgcgacgtt catatttttt cgggcatagg tgtccccaaa
     3241 agtgatcaat acgttataag tgccattgga taggtcttga gtgaagaacc tagtttgtga
     3301 actaaaatta atatcacggt ttatattgtt ccttccgtta gaagccccac gatctcttga
     3361 tccgatgtca ttggtgttcg cccatccaaa attaccagcc ctagtggtgt tggaaacccg
     3421 tgtataccct ggccatactg ccgaggtgtc cgttcctaaa tcatattgaa attcagtggc
     3481 accgtcgtta ccgttgtttc cacctccact taccgcaaca ggcttgtaca cacgtatcca
     3541 atcgacccag aagatatttt tgctatcatc ggccagttct tcttgagtag ccgcgggcct
     3601 ccaatctgtt tgattttcac aatcaataat aatatttgtt gcctgattca accctgttcc
     3661 gccgagatga ttattaggat caatttcggc tcttgtgacc gttctaacct ttaccccgtc
     3721 aatataatat tccaatgtaa atggatcttt ccaataacat ccataacggt gatatgctga
     3781 acgccatggt gtacctccgt tgtaatacca tgtagcgtct cccataggct gataatccgt
     3841 aaaggggtta cgaataaatg tatggtggct caaatgcatt ctttgggcga accaagttcc
     3901 cccccgatca ctgccatagc cttccataat atcaatttcc tgagtctcgt cgtcagtcaa
     3961 ggtccagaaa gcatttgcta gtacctggtc cattatcttg gccttaattt ccatatacac
     4021 cgggtactgg atcttatttt tggaggtgat aattccgttg taagattttc cattccctgc
     4081 tggttgtgcc tgaattgcca attgagaacc attggtccat gcctgcggcg cattaaaaat
     4141 tgttgatcca ggaccagtcc atccattgat ataggaaggt ttccatttac tagtaaaggc
     4201 agtaggccta tttccttcac tacttgtgta attaaaacta tccgaaacat tatcctgtaa
     4261 ttgccaagtc ataccatttc cgggattggc aggtacagga attccgttcc agtcctgtgc
     4321 agcgatagag ccacacaaaa gaaaaataaa atacagtaaa agataatttt ttttcat
//
