LOCUS       FP476056               21601 bp    DNA     linear   BCT 27-MAR-2015
DEFINITION  Zobellia galactanivorans strain DsiJT chromosome, complete genome.
ACCESSION   FP476056
VERSION     FP476056.1
DBLINK      BioProject: PRJEB8976
            BioSample: SAMEA3315864
KEYWORDS    .
SOURCE      Zobellia galactanivorans
  ORGANISM  Zobellia galactanivorans
            Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Zobellia.
REFERENCE   1
  AUTHORS   Thomas,F., Barbeyron,T., Tonon,T., Genicot,S., Czjzek,M. and
            Michel,G.
  TITLE     Characterization of the first alginolytic operons in a marine
            bacterium: from their emergence in marine Flavobacteriia to their
            independent transfers to marine Proteobacteria and human gut
            Bacteroides
  JOURNAL   Environ. Microbiol. 14 (9), 2379-2394 (2012)
   PUBMED   22513138
REFERENCE   2  (bases 1 to 21601)
  AUTHORS   Genoscope -,C.E.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Genoscope - Centre National de Sequencage :
            BP 191 91006 EVRY cedex - FRANCE (E-mail : seqref@genoscope.cns.fr -
            Web : www.genoscope.cns.fr)
FEATURES             Location/Qualifiers
     source          1..21601
                     /organism="Zobellia galactanivorans"
                     /mol_type="genomic DNA"
                     /strain="DsiJT"
                     /db_xref="taxon:63186"
     gene            complement(1..2538)
                     /gene="bgaF"
                     /locus_tag="ZOBELLIA_4655"
     CDS             complement(1..2538)
                     /gene="bgaF"
                     /locus_tag="ZOBELLIA_4655"
                     /EC_number="3.2.1.23"
                     /function="Hydrolyzes the terminal non-reducing
                     beta-D-galactose residues in beta-D-galactosides"
                     /note="Enzyme belonging to the family 2 of glycoside
                     hydrolases (GH2) which catalyzes the hydrolysis of the
                     beta-1,4 linkage at the terminal non-reducing of
                     beta-D-galactosides, releasing a beta-D-galactose residue;
                     Signal peptide of lipoprotein cleaved between residues 17
                     and 18; Possibly localized in the outer membrane; High
                     confidence in function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="GH2"
                     /protein_id="CAZ98790.1"
                     /db_xref="GOA:G0L4M6"
                     /db_xref="InterPro:IPR003344"
                     /db_xref="InterPro:IPR006101"
                     /db_xref="InterPro:IPR006102"
                     /db_xref="InterPro:IPR006103"
                     /db_xref="InterPro:IPR006104"
                     /db_xref="InterPro:IPR008979"
                     /db_xref="InterPro:IPR013781"
                     /db_xref="InterPro:IPR013812"
                     /db_xref="InterPro:IPR017853"
                     /db_xref="UniProtKB/TrEMBL:G0L4M6"
                     /translation="MIKHFLYVVITAVLLSSCGREPSNDSQFNVMTDFNFDWEFRLVDS
                     TGQNDDWKNIRLPHDWSVEASFDSINGEGATGYLPGGVGIYRKKFPLQLKSDEKAYILF
                     DGVYNNSEVSLNGKEVGEHPYGYSPFYYDISPYLNSEKDTNLIEVKVDRTRYADSRWYT
                     GSGIYRDVKLLVKNKLHIPIWGVFVTTPKIEEKQAEVSVSVQIQNDFPEDKKAELRMEI
                     YTRDGQKVNSHIQPIEVRGNNQSLQLQSVSLENPILWDVDKPYLYKVVSSILYEGKLID
                     QQETAFGVRSIRFDPDQGFFLNGKNRKIKGVCLHHDGGLVGAAVPDGVWRRRLQKLKEA
                     GCNAIRISHNPGSQAFLDLCDEMGFLVQDEFFDEWDNPKDKRKNMNEQSVDYITRGYTE
                     HFQEWAEKDLKNTVLAHRNHPSIIQWSIGNEIEWTYPRNAEATGFFNNMEWQGNYFFSE
                     PPYSVEQIKEKLNTLPKEKYDIGVTAKKLAKWTRELDTTRYVTANCILPSASHLSGYAD
                     ALDVVGYSYRRVIYDYGHKNYPDKVIMGTENLPQWHEWKAVMERPFISGLFLWTGIDYM
                     GESNGGWPKKGTQSGLLDQAGFEKTSYHMMKTLWNDEPHLHLTTQSLDKSIYKLNEAGE
                     PVEKKENGWEKALWFWHESNEYWNYETNEPTVVEVYSNCDEVELFLNDASLGRKQLKDF
                     DDRIYKWSVPFVAGELRAEGFINGKKVGAEASLKTAQVPYAVQLSVDKNQLSANAYDVA
                     HIVAQIVDEKGNPVRHGNAEISFSITGDVRLLGVDNGSARNVQDYQSTKIVTDKGRCLL
                     IVQSNFNVGDVRIAAESEGLKTGELMLKLKAVD"
     gene            complement(2654..3670)
                     /gene="rhaT2"
                     /locus_tag="ZOBELLIA_4656"
     CDS             complement(2654..3670)
                     /gene="rhaT2"
                     /locus_tag="ZOBELLIA_4656"
                     /function="L-rhamnose:hydrogen symporter activity"
                     /note="L-rhamnose/proton symporter is involved in the
                     uptake of L-rhamnose across the boundary membrane with the
                     concomitant transport of protons into the cell. Can also
                     transport L-mannose and L-xylose, but at reduced rates.
                     Contains ten transmembrane helices; Localized in the
                     cytoplasmic membrane; High confidence in function and
                     specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter"
                     /protein_id="CAZ98791.1"
                     /db_xref="GOA:G0L705"
                     /db_xref="InterPro:IPR004673"
                     /db_xref="UniProtKB/TrEMBL:G0L705"
                     /translation="MTEGVLWAIFAGLMLGLYALPEKFTKGFKYENTWSLFFLLTMFVV
                     PIIASLTLIQGFGEIFGNMPTDIWVKMGLTSFLWGIGVMMWSKAINHIGLSLGFSIFIG
                     TIILVGSLLPFIEDGLPPSNVLTFILLGILVVLLGVILNGKAGLTREKDEAKIASEGAP
                     AKGSMVTGIMIAVVGGLLATGFSYANAAGRPYLHEASVAQGNADWITAVAVMFPIFISG
                     GIVMTAYFLWQLNAKNAWGEFKTPAFGKNLFLILIMAVFHYAASALFAYAAFKLGASGN
                     TVGYAIFNTASVATAIVSGIVTKEWVSASPKARNLLYGGLGCMVVGIIIVAYANGLG"
     gene            complement(3731..4774)
                     /gene="mroA6"
                     /locus_tag="ZOBELLIA_4657"
     CDS             complement(3731..4774)
                     /gene="mroA6"
                     /locus_tag="ZOBELLIA_4657"
                     /EC_number="5.1.3.3"
                     /function="Alpha-D-aldose = beta-D-aldose"
                     /note="Aldose 1-epimerase or Mutarotase is responsible for
                     the anomeric interconversion of aldoses between their
                     alpha- and beta-forms; It is active on D-glucose,
                     L-arabinose, D-xylose, D-galactose, maltose and lactose;
                     Localized in cytoplasm; High confidence in function and
                     specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="Aldose 1-epimerase"
                     /protein_id="CAZ98792.1"
                     /db_xref="GOA:G0L4M7"
                     /db_xref="InterPro:IPR008183"
                     /db_xref="InterPro:IPR011013"
                     /db_xref="InterPro:IPR014718"
                     /db_xref="InterPro:IPR015443"
                     /db_xref="UniProtKB/TrEMBL:G0L4M7"
                     /translation="MKTTQTDFGVLSGENIYRYTLENDNGLSVKISNYGATITSILLPQ
                     KTGKTDEIVCGFDTFESYFSEAYQANAPYFGGTVGRYCSQIKDARFSLNGKEYQLARIV
                     GDNNLHGGKIGFDKKIWKVKAFSPEVASVQMELESKHLEEGFPGNVTVQVTFTLTNDNE
                     LKIRYGAITDQDTPFTITNHTYFNLSGFAENVEGHTVMVDADTKQLWDETGAATGENVS
                     VAGKVDDLRSPKTIKEVHEAMGDGFEHFYLFEDKGFELGKVAEISEPKSGRTMEVFTTE
                     PGMLLYTGKYTSDELKRESGLQYGKYRGFCCETHRYPNGPNISDAPKTILKVGEDYDST
                     TIFKFNW"
     gene            complement(4812..5906)
                     /locus_tag="ZOBELLIA_4658"
     CDS             complement(4812..5906)
                     /locus_tag="ZOBELLIA_4658"
                     /EC_number="4.2.1.6"
                     /function="D-galactonate = 2-dehydro-3-deoxy-D-galactonate
                     + H2O"
                     /note="Galactonate dehydratase is involved in the galactose
                     metabolism. It converts the D-galactonate to
                     2-dehydro-3-deoxy-D-galactonate; Contains a N-terminal
                     domain with a Enolase N-terminal domain-like fold and a
                     C-terminal TIM alpha/beta barrel fold; Belongs to the
                     Mandelate racemase / muconate lactonizing enzyme family;
                     Localized in the cytoplasm; High confidence in function and
                     specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="Galactonate dehydratase"
                     /protein_id="CAZ98793.1"
                     /db_xref="GOA:G0L4M8"
                     /db_xref="InterPro:IPR001354"
                     /db_xref="InterPro:IPR013341"
                     /db_xref="InterPro:IPR013342"
                     /db_xref="InterPro:IPR029017"
                     /db_xref="InterPro:IPR029065"
                     /db_xref="UniProtKB/TrEMBL:G0L4M8"
                     /translation="MGTSIKSVDCKLFRVPLPEVMNDAKHGDHTHFELITSTITLEDGS
                     TGTGYTYTGGKGGNAIKAMVDYDIAPALIGKDGSDIDGIYDFLEWHMHYVGRGGIVSFA
                     VSTIDIALWDIRCKKAEKPLWQMAGGAGNTCKAYCGGIDLQFPIPKLLKNMEGYLSAGF
                     NAVKIKIGRENLDEDIERIKAVREFIGPDVTFMVDANYSMTVDKAIEAINRFREYDITW
                     FEEPIIPDNYIGFSQIADATGFPLAMGENLHTIHEFEYAMDQAKLSFVQPDASNCGGIT
                     GWLAAARLADKHNIPACSHGMQELHVSLVSAQPNSGWLEVHSFPIDQYTKRPLVVENHL
                     AVAPDTPGVGVEFDWEKLAAFQSK"
     gene            complement(5907..7361)
                     /gene="aldA"
                     /locus_tag="ZOBELLIA_4659"
     CDS             complement(5907..7361)
                     /gene="aldA"
                     /locus_tag="ZOBELLIA_4659"
                     /EC_number="1.2.1.22"
                     /function="(S)-lactaldehyde + NAD+ + H2O = (S)-lactate +
                     NADH + H+"
                     /note="Lactaldehyde dehydrogenase is involved in the
                     catabolism of L-fucose and L-rhamnose and is induced by
                     these sugars. AldA catalyzes the NAD-dependent oxidation of
                     lactaldehyde into lactate; Forms a tetramer; Belongs to the
                     aldehyde dehydrogenase fold family; Localized in the
                     cytoplasm; High confidence in function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="Lactaldehyde dehydrogenase"
                     /protein_id="CAZ98794.1"
                     /db_xref="GOA:G0L706"
                     /db_xref="InterPro:IPR015590"
                     /db_xref="InterPro:IPR016160"
                     /db_xref="InterPro:IPR016161"
                     /db_xref="InterPro:IPR016162"
                     /db_xref="InterPro:IPR016163"
                     /db_xref="InterPro:IPR029510"
                     /db_xref="UniProtKB/TrEMBL:G0L706"
                     /translation="MSGIKEYQLFIDGKWVTSTSGETIDIINPSTEEVVARVQNGTVEE
                     AHLALQAAEKAQKEWKKLPARSRAELLYKLADEIKANSDFLAELLVKEQGKLLKVAKGE
                     VAVTASFIEYACEGARRIEGDIVPSDNPNEQIWIQKIPRGVVVAITAWNFPLALAGRKL
                     GPALVAGNTIVIKPTSETPLATLELGNLAKKVGIPDGVINILTGPGRAMGNALVESPIT
                     KMVTMTGSTPVGQSIARAAAQNLTHVQLELGGKAPFIVFEDADIDAAVDAALHSRFDNC
                     GQVCTCNERMYVHEGIYDAFMEKFIAKTKAIKVGDPMLEDTDMGPKVNASELKHMEHLV
                     AVSLEEGATLATGGKKLEGGAFEKGFWFEPTVLTNVTQNMTIVHEESFGPILPVLKFKT
                     FEEVVGYANDCEYGLAAMVFTNDMNTIMKCNDELEYGEIYVNRGHGEQHQGFHNGYKLS
                     GSGGEDGKYGFEQYMEKKTFYIRHKA"
     gene            complement(7375..8763)
                     /locus_tag="ZOBELLIA_4660"
     CDS             complement(7375..8763)
                     /locus_tag="ZOBELLIA_4660"
                     /EC_number="3.1.6.-"
                     /function="RO-SO3- + H2O = ROH + HSO4-"
                     /note="The family 1 of sulfatases is composed of enzymes
                     that require the posttranslational oxidation of a conserved
                     cysteine (or serine) to a catalytic formylglycine to
                     hydrolyze various sulfate ester substrates; Belongs to the
                     family 1 of sulfatases (S1: formylglycine-dependent
                     sulfatases), subfamily 19; Signal peptide cleaved between
                     the residues 22 and 23; Localized in the periplasmic space;
                     Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="Sulfatase, family S1-19"
                     /protein_id="CAZ98795.1"
                     /db_xref="GOA:G0L4M9"
                     /db_xref="InterPro:IPR000917"
                     /db_xref="InterPro:IPR017849"
                     /db_xref="InterPro:IPR017850"
                     /db_xref="InterPro:IPR024607"
                     /db_xref="UniProtKB/TrEMBL:G0L4M9"
                     /translation="MKWSRRVFLFFASFTMLFSGWAQDKPNIVFILSDDAGYADFGFHG
                     SKEFKTPELDKFAKNAVRFSQAYVSAAVCGPSRAGLLTGKYQQKFGFEENNVPGLMSKN
                     GLTGDDMGLPLDQKTIADYLKEQGYRTALFGKWHQGNADRFHPTKRGFDEFYGFRGGAR
                     SYMPFGADNELTRNEDRLERGFGGFQEHEGYLTEELADEAIAFIERNQKNPFFVYLAFN
                     AVHTPMEAKEEDLKKVKGLSGKRKTLAAMTIAMDRACGKVLDRIKALGLDKNTLIVFTN
                     DNGGPSDANESSNLPLSGTKANHLEGGIRVPFLMSWPGKLKPNTVYENPISTLDLLPTF
                     VTAAGGDVNAIDGLDGVDLLPYLQGKKQGRPHETLYWKKEARGAIRHNDWKLVRFPDRP
                     AELYNIKEDISETNDLASKYPQKVRELYQMLFNWELTLERPLWQLERRFEGLAAKRMDS
                     YRKH"
     gene            complement(8809..9579)
                     /gene="kduD1"
                     /locus_tag="ZOBELLIA_4661"
     CDS             complement(8809..9579)
                     /gene="kduD1"
                     /locus_tag="ZOBELLIA_4661"
                     /EC_number="1.1.1.125"
                     /function="2-deoxy-D-gluconate + NAD+ =
                     3-dehydro-2-deoxy-D-gluconate + NADH."
                     /note="Enzyme that belongs to the Pentose and glucuronate
                     interconversions pathway; Belongs to the short-chain
                     dehydrogenases/reductases (SDR) family, Glucose/ribitol
                     dehydrogenase subfamily; Most dehydrogenases possess at
                     least 2 domains, the first binding the coenzyme, often NAD,
                     and the second binding the substrate. This latter domain
                     determines the substrate specificity and contains amino
                     acids involved in catalysis; Localized in the cytoplasm;
                     High confidence in function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxy-D-gluconate-3-dehydrogenase"
                     /protein_id="CAZ98796.1"
                     /db_xref="GOA:G0L707"
                     /db_xref="InterPro:IPR002198"
                     /db_xref="InterPro:IPR002347"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR020904"
                     /db_xref="UniProtKB/TrEMBL:G0L707"
                     /translation="MSILNQFSLKGKTALVTGCKRGIGKAMAVALAEAGADIIGVSASL
                     EKHGSMVGQEVEATGRKFKAYTCDFSNREALYEFIKEVKNDFPKIDILVNNAGTILRAP
                     AVEHSDEYWDKVIEINQTAQFVLTREIGKEMVARGEGKIIFTASLLTFQGGITVPGYAA
                     SKGAIGQMTMAFANEWAAKGVNVNAIAPGYISTDNTEALRNDPERSASILSRIPAGRWG
                     KPEDFAGPIVFLSSDAAAYMHGVTMLVDGGWMGR"
     gene            complement(9604..10620)
                     /locus_tag="ZOBELLIA_4662"
     CDS             complement(9604..10620)
                     /locus_tag="ZOBELLIA_4662"
                     /EC_number="1.1.1.-"
                     /function="Oxidoreductase activity"
                     /note="The long chain dehydrogenases/reductases are
                     zinc-containing enzymes. Zinc-dependant enzymes are dimeric
                     or tetrameric enzymes that bind two atoms of zinc per
                     subunit where one of the zinc atom is essential for
                     catalytic activity while the other is not. The catalytic
                     zinc is coordinated by two cysteines and one histidine;
                     Contains a C-terminal domain having a classical
                     Rossman-fold that reversibly binds NAD(H); The N-terminal
                     domain of alcohol dehydrogenase-like proteins have a
                     GroES-like fold; Localized in the cytoplasm; Family
                     membership"
                     /codon_start=1
                     /transl_table=11
                     /product="Long chain dehydrogenase/reductase"
                     /protein_id="CAZ98797.1"
                     /db_xref="GOA:G0L4N0"
                     /db_xref="InterPro:IPR002085"
                     /db_xref="InterPro:IPR002328"
                     /db_xref="InterPro:IPR011032"
                     /db_xref="InterPro:IPR013149"
                     /db_xref="InterPro:IPR013154"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="UniProtKB/TrEMBL:G0L4N0"
                     /translation="MKATVYKGNKTFSVIEKEIEAPEKGEVRIKVAYSGVCGTDVHIYH
                     GMMDKRVAMPQTIGHEMSGIIDAVGEGVEGYAVGDKVVVRPLDDRLVKASDKGFNHICE
                     DLKFIGIDSPGSMQQYWNVPAFVLHKLKAETDLKLAALIEPLSVATHDVRRSGLVAGET
                     AVVLGGGPIGLLVAMVAKEVGANVIVSEVNEKRIAKAQSMGLHAVNPTKVDLVEYVKEQ
                     TDNRRADVVFEVAGVQPALDIMCEVAGIRGRILMVAIHGEKKPVDLFKFFWKELSLIGA
                     RVYEKEDYEKSIDLITKNELPFEDMITDVQPLSNIQQVFENIDNNPDGMKVLMDCQQ"
     gene            complement(10634..11860)
                     /locus_tag="ZOBELLIA_4663"
     CDS             complement(10634..11860)
                     /locus_tag="ZOBELLIA_4663"
                     /note="Contains a lipoprotein signal peptide cleaved
                     between the residues 18 and 19; Localized in the outer
                     membrane"
                     /codon_start=1
                     /transl_table=11
                     /product="GH117"
                     /protein_id="CAZ98798.1"
                     /db_xref="GOA:F0V1E3"
                     /db_xref="InterPro:IPR006710"
                     /db_xref="InterPro:IPR023296"
                     /db_xref="PDB:3P2N"
                     /db_xref="UniProtKB/TrEMBL:F0V1E3"
                     /translation="MNKYSQFLIFAAVLVSACNSPKTTKEMKSTDDCPEKVTFTPEQID
                     HLGITDTNHLSAASKRALKWPTDLGNEWFIQFGPLQPLKGDLAYEEGVVRRDPSAIIKE
                     NGKYYVWYSKSTGPTQGFGGDIEKDKVFPWDRCDIWYATSEDGWTWKEEGPAVTRGEKG
                     AYDDRSVFTVEIMKWEDKYYLCYQTVKSPYNVRVKNQVGLAWADSPDGPWTKSEEPILS
                     PADNGVWKGEEQDRFAVIKKGDFDSHKVHDPCIIPYKGKFYLYYKGEQMGEAITFGGRQ
                     IRHGVAIADNPKGPYVKSPYNPISNSGHEICVWPYNGGIASLITTDGPEKNTIQWAPDG
                     INFEIKSVIPGVNAHAIGLNRTADVEKEPTEILRWGLTHIYNNGDYQSIMRFSSERKTR
                     HVAKGVKKQ"
     gene            complement(11912..12643)
                     /locus_tag="ZOBELLIA_4664"
     CDS             complement(11912..12643)
                     /locus_tag="ZOBELLIA_4664"
                     /function="Transcription regulator activity"
                     /note="The AraC-type transcriptional regulators are usually
                     involved in the positive regulation of catabolic, metabolic
                     and transport operons; Contains a C-terminal AraC-type HTH
                     DNA-binding domain involved in the binding of the protein
                     to the operator sequences in the DNA and a N-terminal
                     domain presumed to interact with effector molecules and may
                     be involved in dimerization; Localized in the cytoplasm;
                     Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUP
                     ERFAMILY|0035607"
                     /protein_id="CAZ98799.1"
                     /db_xref="GOA:G0L708"
                     /db_xref="InterPro:IPR009057"
                     /db_xref="InterPro:IPR018060"
                     /db_xref="InterPro:IPR020449"
                     /db_xref="UniProtKB/TrEMBL:G0L708"
                     /translation="MKLVSYLNQKKYHMDATIVIREDALGDVENQNGVLQNGELHINVD
                     FVDDYGDDVQVYNGVFEKGLNMPAILLDAFYALMAKVKVLEFGMDQNISEHFKILKGKA
                     GKMIFAGDVDEKNTLGQPGLTVNLSALEAGDREFVEKVLDFVEENISDNTYWVDDLSFD
                     MNTSRSTFFRKLKKITGHAPKDFMRNLRLMKAGELLSRGELRIAEVSYQVGFGDPNYFS
                     KCFRKFYGTSPSDYIAMNRAN"
     gene            complement(12888..13679)
                     /locus_tag="ZOBELLIA_4665"
     CDS             complement(12888..13679)
                     /locus_tag="ZOBELLIA_4665"
                     /function="Transcription regulation"
                     /note="IclR-type transcriptional regulators display a
                     Helix-Turn-Helix motif at the N-terminus. Localized in the
                     cytoplasm.; Specificity unclear"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-SUPERFAMILY|0040266"
                     /protein_id="CAZ98800.1"
                     /db_xref="GOA:G0L4N2"
                     /db_xref="InterPro:IPR005471"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR014757"
                     /db_xref="InterPro:IPR029016"
                     /db_xref="UniProtKB/TrEMBL:G0L4N2"
                     /translation="MKKPKAELKSTYNVPNLERGLSIIELLATRTKGMTLAEITETLSI
                     TKSSAFRIVSTLIFKNYLQKNETTKKITLSRKMLTLGISSMNEQSIVEHSIDVMRALRD
                     ELKESVMLGVLLGSTGTILEQVPSSYPVKLFVEPGTQFDLHSSVGGKCILAHIPSEEAD
                     MALNGKPLTKFTENTITSKKQFKRVLKEVREKGYAVDNSEDIEGINCVGAPIFNEYGYP
                     VAALWITAPYGRLPASQFESKGKIVMKYALVISTKLGYLTH"
     gene            complement(13775..15418)
                     /locus_tag="ZOBELLIA_4666"
     CDS             complement(13775..15418)
                     /locus_tag="ZOBELLIA_4666"
                     /note="Contains a N-terminal transmembrane segement
                     followed by three tetratricopeptide repeats (TPR) and a
                     C-terminal LuxR-type HTH domain. Between the TPR and HTH
                     domains a second transmembrane segement is present; This
                     Protein could interact with the DNA (transcriptional
                     control or transcription factor); Probably localized in the
                     cytoplasmic membrane; Conserved hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|GerE"
                     /protein_id="CAZ98801.1"
                     /db_xref="GOA:G0L4N3"
                     /db_xref="InterPro:IPR001440"
                     /db_xref="InterPro:IPR011990"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR013026"
                     /db_xref="InterPro:IPR013249"
                     /db_xref="InterPro:IPR016032"
                     /db_xref="InterPro:IPR019734"
                     /db_xref="UniProtKB/TrEMBL:G0L4N3"
                     /translation="MVLQVKHLVSFIMVILCWVPLSRLDAQQLSDTLEGLQGEAFFEEA
                     RKLRRDNPEEALVLLQEKHEELLKKGDTVNAVKALLEMPYINGQHVDYTKSYDTLWYAL
                     FLADSVDDDALKASVYNGLGRLYSFFKRKDEALEYLQTSLEINRKLIDRGKLQKADLVE
                     NYYLISATHRILNQHHLARVYLDSCFVNYAPIDGQLPMAYLHFEQAFQLYLDNKNEEAL
                     LLLGEVESWFLKNGPSFLVLVYSDMGDVYANMSNYKQSEVYYKKALAISEEYNSHVDFS
                     SLVYEKLADLYESLGAYENAYKNQKKAKAIDAQFFDSRTAINQSLLEIKDEFRVEKEKQ
                     ERLVRKQRLEKLEQADKISMLQRIILLGSLVFMAILGLIYFRSIRTRHLTEKQMLRRNK
                     QLEIKKGKELLDLKNKELAVSALQMVEKDEFLKEIAERLKEAEGISDKPEVKQVLKSIS
                     HNRSDNWEEFRLRFTAVNEDFYNKITTMYPKLSQADQKLCALIKLNFSSKEIARLLGIS
                     VESVHTTRYRLRKKMKLERSVNLEEYISSL"
     gene            complement(15422..15667)
                     /locus_tag="ZOBELLIA_4667"
     CDS             complement(15422..15667)
                     /locus_tag="ZOBELLIA_4667"
                     /note="Sequence that displays some similarities with the
                     C-terminal end of several paralogous proteins; Putatively
                     localized in the cytoplasm"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /protein_id="CAZ98802.1"
                     /db_xref="UniProtKB/TrEMBL:G0L709"
                     /translation="MLADNNKQSEVETTRSDAGIGTFLKGNGKGTFVLVPNLQSGFFAD
                     KDVRNIAALNTKGSKFILVANNDGYHNLFKLNQDIE"
     gene            complement(16650..18398)
                     /locus_tag="ZOBELLIA_4668"
     CDS             complement(16650..18398)
                     /locus_tag="ZOBELLIA_4668"
                     /function="Transporter activity"
                     /note="Protein probably involved in nutrient binding and
                     belonging to the SusD/RagB family; Contains a lipoprotein
                     signal peptide cleaved between the residues 20 and 21;
                     Localized in the outer membrane; Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family lipoprotein"
                     /protein_id="CAZ98803.1"
                     /db_xref="InterPro:IPR011990"
                     /db_xref="InterPro:IPR012944"
                     /db_xref="UniProtKB/TrEMBL:G0L4N4"
                     /translation="MKNKFFANSFAISILVLITSCSDDFLNEEPVGTVNELTLANEEGV
                     NGLLIGAYSLIDGYSSNGSPKGGQGEASGTNWIWGDVPSDDMHRGDTNGGWSQINDIER
                     YEVRSDNEWLNGAWGVNFDGVARSNDVLRVLATAGEVISTEKADQIEAEAKFLRAWFHF
                     QLRIKFEKVPYITEDVDPALVKNDREIWDDIEDDLQFGIDHLDPAPSEVGRASQWAAKA
                     LKARVHLFQSEFAEAKPLLDNIIEEGPFQLEPHFYNNFDEEHQNNGESIFEIQYSVNDG
                     AGVHNSGVDHQTLYPNVEGLGYSYSAPSFDLFNAYKVEDNGLPLLDSFQDDLLLEDYQI
                     SDTEEFTPTSHLLDPRVDWTIGRRGIPFLDWGPMSGSDWMSDQLNMGPFMNKKNMFYKR
                     NRGVISTESGYWAPGVNGNNFRLLRLGHVLLWRAEVAVEENDLPTALALVNRIRQRAAD
                     DIVMGKVSNTTFEEGNPIIVDYSQPAANYKLGLYDSFPSVEYARMAVRHEMRLEFALEG
                     MRFYDLVRWGIADSTLNNYLSSELKDNKLPWLSGSSFTSGKNNYWPIPQVQLDLQPGVL
                     TQNSSL"
     gene            complement(18419..21601)
                     /locus_tag="ZOBELLIA_4669"
     CDS             complement(18419..21601)
                     /locus_tag="ZOBELLIA_4669"
                     /function="Receptor and transporter activity"
                     /note="Protein localized in the outer membrane involved in
                     uptake of macromolecules that are too large to diffuse via
                     the outer membrane porins channels or are encountered at
                     very low concentrations; Contains a carboxypeptidase
                     regulatory domain (27-99) and the Plug module (115-218)
                     acting as a channel gate; The signal peptide is cleaved
                     between the residues 25 and 26; Family membership"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="CAZ98804.1"
                     /db_xref="GOA:G0L710"
                     /db_xref="InterPro:IPR000531"
                     /db_xref="InterPro:IPR008969"
                     /db_xref="InterPro:IPR012910"
                     /db_xref="InterPro:IPR014766"
                     /db_xref="InterPro:IPR023996"
                     /db_xref="InterPro:IPR023997"
                     /db_xref="InterPro:IPR027804"
                     /db_xref="UniProtKB/TrEMBL:G0L710"
                     /translation="MKLLKFLKMGVFLAIFFLFSSHIQAQTMVTGTVSDEQGPIPGVNI
                     LIKGTSNGVTTDFDGNFSIEASPEDVLVISYLGYGTQNIKVGNQTTLNITLLTDQQQLD
                     EVVVIGYGTRSKDALTSAVATVDKNYLEQQPVSNVSKALQGSASGVTTISASTPGGDAE
                     VRIRGMGTINNNGPLWVVDGVQGAQSPPPGQIESIQILKDASSTAIYGARGANGVILVT
                     TKTGKKNQAVKLDISVKTGMSFSDAKYDIMMDPQLIGEMLWLELENDGLPQTNAHFGSG
                     ATPVLNEYLYPNGGVSGAANVNPALYDQVNYPITKSNPQGTDWLDEIYQHGLIQNYDLS
                     LMGGSEKTSYSFQAGFLEENGILKNTSFNRISFRSNVDSNPFEWLNIGQRLGVTYGETK
                     GWGGNNSHNNLFNQIYLASPLVPVRDIAGNWAGGVVGGANINDGPNPVGFLDRLKDNSN
                     KKYNITGNVYAAIEPIKNVTIKTLLGYDVDLFNNFSPSFAAWEQNTGAKSTTLTESSAN
                     ETRWNWTNTFNYKNTFGEDHSVDFLFGMESSRIAYRTISASRQEYLSNDINFLVLDAGA
                     SNQLNNGSGFASTLFSYFSRINYDYAKKYILDLTVRRDGSSRFGAENRYGIFPAASLGW
                     VASKENFLSGTEDWLSYLKFRASWGQSGNDQIGNYNSFTTYESDPGSSYYAIGGSDNTI
                     TQGYQSLAIGNPNAKWETTTSTNIAFDATLFRKLDLSVDFWKKDTEDMLFTVAIPQVVG
                     DVIEPSVNIGGMSNKGVDISLSYRTSALDDWFTLNMSTTFTAYKNEVTKLSDNGNEFIQ
                     GFPTRQQIYTRTEVGRSFPEYYGYVVDGIFQTQSEADAHATNGTYNEPGNLKIRDVDGD
                     GEITPEDRTYIGNPHPDFTAGFRLGMGFGNIDVSATLYASVGNDLANYTSRFTKYGLFE
                     GPNSPERLYNSWGSPYLEDNNDATLPKASTSTAFEQNASTLYIEDGSFLRIQNLQVGYT
                     LPDEIIEKLHLKHMKLYVIGSNLFTFTNYSGLDPEISAKRENGVPQEIDRGVDVGTWPI
                     SKQIMLGLNISL"
ORIGIN
        1 ctagtccact gccttgagct tcaacatcaa ctcacccgtt ttcaaccctt ctgattctgc
       61 tgctatcctt acgtcgccta cgttgaaatt cgattgtacg ataagaagac agcgcccctt
      121 atcggttacg atcttggtcg attggtaatc ttgaacattc ctggccgaac cattgtctac
      181 ccccaacaat cttacatcgc cggtaatgga gaaagaaatt tcagcgttac catgtcttac
      241 agggttccct ttttcatcta caatttgggc cactatatgg gccacatcgt aggcattggc
      301 agatagttgg ttcttatcta cggaaagttg aacggcataa ggcacttgag ccgtctttaa
      361 tgaagcctct gcccctactt tctttccatt tataaaacct tccgccctaa gctcaccggc
      421 aacaaagggc acagaccact tataaatgcg atcgtcaaaa tccttaagtt gttttctgcc
      481 tagcgaagca tcgtttaaaa agagttccac ttcatcgcaa ttggaataga cctctacaac
      541 ggtcggttcg ttggtctcgt agttccaata ttcattactt tcatgccaga accacagtgc
      601 cttctcccac ccgttctctt tcttttccac gggctcgccc gcctcgttca atttatagat
      661 cgatttgtct aaagactgtg tggtcaggtg caaatgcggt tcgtcgttcc acaaagtttt
      721 catcatgtgg tacgaagtct tttcaaaacc ggcctgatcc aataaaccgc tttgagtacc
      781 ttttttgggc caacctccat tcgattcgcc catataatcg attcccgtcc aaaggaagag
      841 gccggaaata aatgggcgct ccatcaccgc tttccattcg tgccattgcg gtagattttc
      901 ggttcccatg atgactttat ccgggtaatt cttgtggcca taatcataaa tcacccttct
      961 ataactgtag cctactacgt cgagtgcatc ggcatagcct gagagatgac ttgcggaggg
     1021 cagaatacaa ttggccgtaa catatcgcgt agtatctaat tcccgggtcc atttcgccaa
     1081 ttttttagcg gttaccccaa tatcatattt ttccttgggc aaggtgttga gtttctcttt
     1141 tatctgttct accgaatacg gtggctctga aaagaagtaa ttgccctgcc attccatatt
     1201 attgaaaaaa ccggtagctt cggcatttcg cggatacgtc cattctattt cgttcccgat
     1261 actccattga atgatactcg gatggtttct atgggccaaa accgtattct ttaagtcctt
     1321 ttcggcccac tcttgaaaat gctcggtata gcctcttgtg atatagtcta cactctgctc
     1381 gttcatattt tttcttttgt ccttcggatt gtcccattca tcaaagaact cgtcctgcac
     1441 caaaaagccc atttcgtcac acaggtccaa aaacgcttgt gagccgggat tgtgagaaat
     1501 tctgatggcg ttacaccccg cttcctttaa cttctgtaga cgccttctcc agacaccgtc
     1561 gggtacggcc gcccctacca atccaccatc gtggtgcaag caaacccctt ttatttttcg
     1621 atttttaccg ttaaggaaaa aaccttgatc agggtcaaaa cggatggacc gtacaccaaa
     1681 agcagtttct tgttggtcta tgagctttcc ttcataaaga attgaactta ccactttgta
     1741 gagataaggc ttatcgacat cccacaaaat tgggttttca agggatacag actgtaattg
     1801 aaggctttga ttattccctc gaacctcaat aggttgtata tgactgttga ctttttgccc
     1861 gtccctagta taaatctcca ttcggagttc cgctttttta tcctcaggaa aatcattctg
     1921 aatttgaacc gacaccgaca cctccgcttg tttttcctct attttcgggg tggttacgaa
     1981 tacgccccaa ataggtatat gcaatttgtt cttcaccaag agttttacgt ctcggtaaat
     2041 acccgaaccg gtgtaccaac gactatcggc ataacgggtt ctatctactt tcacctctat
     2101 caggttggta tctttttccg aattcaaata gggcgagata tcgtagtaaa acggagagta
     2161 tccataggga tgttccccta cttcctttcc gtttagggaa acctcggaat tgttatagac
     2221 gccgtcgaac aagatatagg ccttttcatc cgatttcaat tgaagcggga attttttacg
     2281 atatattccc acacctcctg gaaggtatcc cgtagcccct tccccgttta tagaatcaaa
     2341 agaagcctct acactccaat catgaggaag cctaatattt ttccaatcat cgttttgccc
     2401 cgttgaatcg accaaacgaa actcccaatc gaaattaaaa tcggtcataa cattaaactg
     2461 cgagtcattt gacggctcgc ggccacatga agagagcaac acggctgtaa ttactacgta
     2521 taaaaagtgt tttatcatag gtctaatctt tattttaaag cctgaaataa aaaaagcccg
     2581 attgaatagg aacaaacaac cggactcaac tactaaagaa caatattttt acttgtcaaa
     2641 aacggaacca tcattatcct aaaccattgg catatgctac aattatgata cctaccacca
     2701 tacaaccaag acctccgtat aaaagattcc tggccttggg ggaggcactt acccattctt
     2761 ttgttacgat accgctaacg atggcggtag ctacggaagc cgtattaaaa atggcatatc
     2821 cgaccgtatt ccccgatgcc cccaacttaa aggctgcata ggcaaaaagg gcggatgcgg
     2881 cataatgaaa aaccgccatg atcaatatca gaaacaggtt ttttccgaaa gccggagtct
     2941 tgaattcccc ccatgcattt ttagcgttca attgccatag gaaataggcg gtcataacga
     3001 tacccccact aataaagatc gggaacatta cggctacggc cgttatccaa tcggcattgc
     3061 cctgggccac actggcctca tgcaagtaag gtcgacctgc ggcattggca tagctaaagc
     3121 ccgtggccaa taatccacca acaacggcga tcataatgcc ggtcaccatt gaacctttgg
     3181 ccggcgcacc ttcggaggct attttagctt cgtccttttc tcgggtaagt ccggctttac
     3241 cgtttaagat aacccctaac aacactacta gaataccgag caaaataaag gtcaatacat
     3301 ttgagggagg aagtccgtct tcaataaacg gcaataggga gcctaccaaa attatggttc
     3361 cgataaagat ggaaaaacca agggagagac caatatggtt aatggcctta ctccacatca
     3421 taaccccaat tccccaaagg aaactggtaa ggcccatttt cacccatata tcggtcggca
     3481 tatttccgaa aatttcccca aaaccctgga taagggtcaa agaagcaata atcggcacaa
     3541 cgaacatggt caaaagaaag aagagactcc atgtattctc atacttgaaa ccttttgtaa
     3601 atttttcggg aagggcataa aggcccaaca tcaatccggc gaaaatcgcc cataatacac
     3661 cttcggtcat aactataata tttaattggg gccaagtaca ccggttcggg cataccaggc
     3721 cgtgtttggt ttaccagttg aatttaaaaa tcgtcgtact atcgtaatcc tcccccactt
     3781 ttaagattgt cttcggggcg tctgaaatat tggggccatt tgggtagcgg tgagtttcac
     3841 agcaaaagcc gcgatattta ccgtattgca atcccgattc tcttttgagc tcatctgaag
     3901 tatattttcc ggtgtagagt aacattccgg gttccgtggt gaaaacttcc atggtgcgac
     3961 cacttttagg ttcactaatt tctgccactt tacccagttc aaacccctta tcctcaaaga
     4021 ggtaaaaatg ttcaaagcca tcgcccatag cctcgtgtac ttcctttatg gtctttggag
     4081 agcgcaggtc gtcgaccttg cccgcaacag agacgttttc cccagtggca gctccggttt
     4141 catcccaaag ctgtttggta tcggcatcta ccattacggt gtgaccctca acattttcag
     4201 caaagcccga aagattaaaa taggtgtgat tcgtaatcgt aaaaggcgtg tcttggtcgg
     4261 tgatagcccc ataccgtatt ttaagttcgt tgtcattggt caatgtgaag gtcacctgaa
     4321 ccgtaacatt tcctggaaag ccctcttcca aatgtttact ctcaagctcc atttgaacac
     4381 tggcaacttc aggcgaaaac gcctttacct tccatatttt tttatcgaaa ccgatttttc
     4441 ctccgtgcag gttattatcg ccaactattc ttgccaattg gtattccttt ccattcaaag
     4501 aaaatcgggc atcttttatt tgcgagcaat agcggccaac cgttccccca aaatatgggg
     4561 cattggcttg ataggcctcg gagaaatagc tctcgaacgt atcaaaacca caaacgattt
     4621 catccgtttt ccctgtcttt tgcggtaaaa gaatagaggt aatggtagcc ccatagttgg
     4681 aaatctttac actaagccca ttatcgttct ctaaagtata gcggtaaatg ttttcaccgc
     4741 tcaatacacc gaaatctgtc tgtgtcgttt tcattatcgt aagcgttttt gaatgttcta
     4801 gtcagaatta tttatttcga ttgaaaagct gccagttttt cccaatcgaa ctccactcct
     4861 acccctggcg tatctggtgc tacggcaaga tggttctcta ccaccaacgg tcttttggtg
     4921 tattggtcaa taggaaaact atgtacctct agccaacctg aattcggttg ggcggatacc
     4981 aaactcacat gtagttcctg cattccatgc gaacaggctg gaatattatg cttatcggcc
     5041 aaccgtgcgg cggccaacca acctgtgatt ccaccacaat ttgaagcatc tggctgtaca
     5101 aaggataatt ttgcctgatc catggcatat tcaaattcat ggatggtatg caggttctcg
     5161 cccatagcca aaggaaagcc tgtagcatcg gcaatttgtg agaaacctat atagttatca
     5221 ggaataatcg gttcttcgaa ccaagtgata tcgtattccc taaagcggtt aatagcctca
     5281 atggccttat caacggtcat tgagtagttg gcatcgacca tgaaggtcac atcgggcccg
     5341 atgaactcac gtaccgcttt aatgcgctct atgtcctcat cgagattttc ccttccgatc
     5401 ttaatcttga ccgcgttgaa acccgccgac aaatagccct ccatattctt caaaagtttt
     5461 ggaataggaa actgtaagtc aattccccca caataagctt tacaggtatt acccgcacca
     5521 ccggccattt gccaaagtgg tttttcggcc ttcttacaac ggatatccca cagtgcaata
     5581 tctatcgtag aaacggcaaa ggaaacgata ccccctctac ctacatagtg catgtgccat
     5641 tcaagaaaat cgtaaatccc atcaatatcg gaaccgtcct tacctatcaa ggccggtgct
     5701 atatcgtaat cgaccatggc cttaatggca tttcctcctt ttccacccgt ataggtatat
     5761 ccagtgcccg tactaccatc ttctaaagtg atcgtagaag taatcagttc aaaatgcgta
     5821 tggtctccgt gtttggcatc gttcataact tccggcaagg gaaccctgaa caatttacag
     5881 tcaaccgatt ttatacttgt acccatttat gccttgtgtc taatataaaa cgtctttttc
     5941 tccatgtact gctcaaagcc gtacttccca tcttcacctc ccgagccact tagcttatat
     6001 ccgttatgga agccttggtg ttgttcacca tgaccacggt ttacgtagat ttcaccgtat
     6061 tccaactcat cattacactt catgatggta ttcatatcat tggtaaagac catggccgca
     6121 agtccgtatt cacagtcatt ggcataaccg accacttctt caaaagtctt gaatttcaag
     6181 acgggaagaa ttggcccaaa agattcttca tgaacaatgg tcatattttg ggttacattg
     6241 gtcaaaaccg taggctcaaa ccaaaatcct ttttcaaaag caccaccttc aagtttttta
     6301 ccccctgtag ccaaagtcgc accttcttct aggctcacgg caaccaaatg ctccatatgc
     6361 tttaattcgg aagcatttac tttaggtccc atatcggtat cctccaacat agggtcacct
     6421 accttaatag cctttgtctt ggcgataaat ttttccataa aggcatcgta aatgccctca
     6481 tgaacgtaca ttctttcatt acaggtacac acctgtccac agttatcaaa acgcgagtgt
     6541 agggcagcgt ctacagcggc atcgatatcg gcgtcttcaa aaacaataaa aggagctttc
     6601 cctccaagtt ccaactgaac gtgcgtaagg ttttgtgccg cggccctggc aatagactga
     6661 cccacgggcg tagaaccggt cattgtcacc attttagtaa taggactttc aacaagggca
     6721 ttacccatgg ccctacccgg accggtcaat atattgatga ccccatctgg aatacccact
     6781 tttttcgcaa gattacccaa ttccaaagta gccaaaggcg tttcggaagt cggttttata
     6841 acgatagtat ttccggcaac caaggctgga cctaatttac gaccggccaa ggccaatggg
     6901 aaattccaag cggtaatggc aaccactacg ccgcgaggta ttttctggat ccaaatttgc
     6961 tcgttagggt tgtcggaagg cacaatatcc ccctcgattc ttctagcccc ttcacaagca
     7021 tattcaataa aagaagcggt aacagccact tcccccttag ctacttttag cagtttcccc
     7081 tgttccttga ccaagagctc ggccaagaaa tcggaattcg ccttaatctc atcggccaac
     7141 ttatacagga gctcggcacg cgaacgggcc ggtaatttct tccattcctt ttgggccttt
     7201 tcggccgcct gtagcgctag atgggcctct tcgaccgtac cgttctgaac ccttgcaacg
     7261 acctcttcgg tagatgggtt tataatatct atggtctcac ctgaggttga ggtaacccat
     7321 tttccgtcga taaatagttg gtattcttta attcctgaca tttttcgtta attattagtg
     7381 ttttctatat gaatccattc gtttggccgc aagaccttca aaccttcttt ccaattgcca
     7441 aagaggtcgc tccaaggtta attcccaatt aaagagcatt tgataaagtt cacggacttt
     7501 ttgaggatat ttagaagcta aatcattagt ttcggatata tcttccttaa tattatagag
     7561 ttccgcaggg cgatcgggaa agcgtaccag cttccaatcg ttatgcctaa tggctcccct
     7621 agcttctttt ttccaataca gtgtttcatg tggcctccct tgcttttttc cttgtaggta
     7681 aggcaggagg tctaccccgt ccaatccgtc gatagcattg acgtcaccac cggccgccgt
     7741 aacaaaagtc ggcaataggt ccaatgtact aattgggttt tcataaaccg tattcggctt
     7801 taatttaccg ggccatgaca ttagaaaagg aacgcgaatc cccccttcta aatggttggc
     7861 cttggtgccg cttaaaggaa ggttcgaaga ttcgtttgca tccgatggcc caccgttatc
     7921 gttagtaaaa acaattagtg tattcttatc gaggcccaat gccttgatcc ggtccaaaac
     7981 cttaccgcaa gccctatcca tggcaatggt cattgccgct agcgtctttc tttttccgct
     8041 taggcctttg acctttttaa ggtcttcttc ctttgcttcc attggggtat gtaccgcatt
     8101 aaaagccaag taaacaaaaa acggattctt ctggttcctc tcgataaatg caatggcctc
     8161 atcggccaat tcctccgtta gatagccttc gtgttcttga aaacctccaa aaccacgttc
     8221 taacctatct tcgtttctcg taagttcgtt atcggctccg aagggcatat aacttcttgc
     8281 acctccgcga aaaccataaa actcgtcaaa accgcgtttg gtcggatgaa accgatcggc
     8341 attcccttga tgccacttcc cgaacaaagc tgttctatac ccctgttcct ttaggtagtc
     8401 ggcaatagtt ttttgatcaa ggggcaagcc catatcgtca cccgtaagac cgtttttact
     8461 catcaaacca ggaacgttgt tctcttcaaa cccaaatttc tgctgatact ttccggttag
     8521 gagtcccgcc cttgacggac cacacacggc cgcagacaca taggcctgcg agaagcgtac
     8581 ggcatttttt gcaaatttgt ctagttcggg cgtcttgaat tctttactcc catgaaatcc
     8641 gaaatcggca taaccggcat cgtcggacaa tataaaaaca atgttcggtt tgtcttgggc
     8701 ccagccacta aagagcatag tgaaagacgc aaagaacaag aacacacgtc ttgaccattt
     8761 catttttcta tattttttgt ggattatttt ttacaattcc cggtaccatt atcttcccat
     8821 ccagccaccg tctaccaaca tggtaacacc gtgcatataa gccgctgcat ctgaacttaa
     8881 aaagactata ggtcccgcaa aatcttcagg tttaccccat ctaccagctg gtattcgcga
     8941 caaaatagac gccgatcttt caggatcgtt ccttaaagcc tcggtattat cggtactgat
     9001 ataacctggc gctatggcat ttacgttcac tcccttagcc gcccattcat tggcaaaggc
     9061 catggtcatc tgaccgatcg cccctttact ggccgcataa ccaggcactg taataccacc
     9121 ttggaatgtc aacaacgaag ccgtaaaaat aattttacct tcccctcggg ccaccatctc
     9181 cttaccgatt tcgcgggtaa ggacaaattg cgccgtttga ttgatttcga ttaccttatc
     9241 ccaatactca tcggaatgtt caactgcagg ggcccgaaga atggtacctg cattatttac
     9301 taggatatcg attttaggaa aatcgttttt cacttccttt ataaattcat ataaagcttc
     9361 cctattacta aaatcacaag tgtatgcctt aaattttctt cccgtggcct cgacttcctg
     9421 ccctaccata ctaccatgct tctccaaaga ggcgctaacc cctatgatat cggctcccgc
     9481 ttctgccaat gctacggcca tagcctttcc tatacctctt ttacaccccg ttaccaaagc
     9541 tgtttttcct ttcaggctaa attgattcaa tatgctcatt ttctatgcgt tgtttccctt
     9601 taattattgt tgacaatcca taagtacttt cataccatca ggattattat ctatattttc
     9661 aaatacttgt tgaatattgc tcaatggctg aacatcggta atcatatcct cgaagggcag
     9721 ttcgtttttt gtaatcaaat cgattgattt ttcatagtcc tccttttcgt ataccctagc
     9781 cccgatcagg ctcaattctt tccagaaaaa cttgaaaagg tctaccggtt tcttttcccc
     9841 atgaatggcc accatcaaga ttcgcccgcg aataccggca acctcacaca taatatccaa
     9901 agcaggctgt acccctgcaa cttcaaaaac cacatcggca cgacggttat cggtttgctc
     9961 cttcacatac tctactaggt ctaccttcgt agggtttacg gcatgtaatc ccattgactg
    10021 ggccttagcg atacgttttt cgttcacttc tgaaacgatg acgtttgccc ctacttcctt
    10081 agctaccatg gccaccaaaa gacctatagg gccgccgccc aaaaccacgg ccgtttcacc
    10141 ggctaccaaa ccacttctac gaacgtcgtg ggtagcaacc gataggggct cgatcaaagc
    10201 ggccaatttt aaatcggtct ccgcctttag tttatgaagg acgaaggcag gtacgttcca
    10261 atactgctgc atagaacccg gactatcgat accgatgaat tttaaatctt cgcaaatatg
    10321 gttaaagcct ttatcggaag cctttaccaa acgatcgtcc aagggacgga ccacaacttt
    10381 atcgccaacc gcgtagcctt caaccccttc gccaacggcg tcgatgattc ccgacatttc
    10441 atgaccaatg gtctgaggca tggccacccg cttgtccatc ataccatgat agatatgcac
    10501 atcggtgcca cacactcctg aataggccac tttaattcta acctcacctt tttccggtgc
    10561 ttcaatttcc ttctcgatta cggaaaaggt tttgtttcct ttgtaaacag ttgctttcat
    10621 caaacttatt ttcctattgt ttttttactc ctttagctac gtgacgtgtt tttctttccg
    10681 aagaaaaacg catgatactc tggtaatcgc cattattgta tatatgcgtc aatccccatc
    10741 gtaaaatttc ggtcggttcc ttttcaacat ccgctgttcg gttcagtccg atagcatggg
    10801 cattgacccc tggtattacc gatttaattt caaagttaat tccgtctgga gcccactgga
    10861 tcgtattctt ttcaggacca tctgtagtga tcaacgaagc aatacctccg ttataaggcc
    10921 aaacacagat ttcatgacca ctattgctaa tagggttata tggagatttc acgtaaggcc
    10981 ctttgggatt gtcggctatg gccacaccat gacgtatctg tcttccgccg aaagtaatcg
    11041 cttcacccat ctgttcccct ttgtaataga gatagaattt ccccttataa ggtataatac
    11101 atggatcatg tactttatgg ctatcaaaat cacctttttt aatcacggcg aaacgatctt
    11161 gctcttcacc cttccacact ccgttatcgg caggactcaa aataggctct tcacttttgg
    11221 tccatggccc gtcgggagaa tccgcccaag ccagacctac ttgattttta acacggacat
    11281 tatagggtga ttttaccgtt tgatagcaga ggtagtactt atcttcccat ttcatgatct
    11341 caacggtaaa aacggagcga tcatcataag cccctttttc acctcttgtt actgcgggac
    11401 cttcttcttt ccacgtccaa ccatcttcgg aggtagcata ccaaatatca catcgatccc
    11461 acggaaaaac cttatctttt tcaatatccc caccaaaacc ttgcgttggc ccggtacttt
    11521 tagaatacca cacgtagtac tttccattct ctttgattat ggcacttgga tcacgacgaa
    11581 cgacaccttc ctcataagcc aaatccccct ttaaaggttg taacggacca aattgaataa
    11641 accattcatt acccaagtcc gtaggccatt tcaaggctct ttttgacgca gcgctcaaat
    11701 ggttggtatc cgtaatgccg agatggtcaa tttgttcggg agtaaacgtc accttctctg
    11761 ggcaatcatc tgtagatttc atttcttttg tagttttggg agaattgcag gccgatacca
    11821 gcaccgcagc aaaaattaaa aattgggagt atttgttcat agttattgat acttgatcta
    11881 aaatttaaat atacatgttt acactactaa actaattggc tcgattcatc gcgatataat
    11941 cggacgggga agttccatag aacttcctaa aacacttact aaaataattg gggtcgccaa
    12001 aaccgacctg atagctgacc tcggcaatcc ggagctctcc acgggaaagc aactctcccg
    12061 ccttcatcag ccttaagttc ctcataaaat cctttggcgc atgacccgtt atttttttta
    12121 attttctaaa aaaggtcgaa cgactggtat tcatgtcgaa tgaaaggtca tctacccaat
    12181 atgtattgtc ggagatattt tcctctacaa aatccaaaac cttttcaaca aactccctat
    12241 cccctgcttc taacgcggaa agattcaccg ttaatccagg ttgacccaaa gtgttttttt
    12301 catctacatc gcccgcaaaa atcattttac ccgctttacc cttcaatatc ttgaagtgtt
    12361 ctgaaatatt ttgatccata ccaaactcta gtactttcac ttttgccatc aaggcataaa
    12421 atgcatcaag taaaatagcg ggcatattca atcctttttc aaagacccca ttatacacct
    12481 gcacatcatc tccgtaatca tcaacaaaat ctacattaat atgtagctca ccgttttgca
    12541 atactccatt ctggttttca acgtcaccaa gagcatcttc ccttataaca attgtagcat
    12601 ccatatggta ttttttttga ttcaaataag aaactagttt catatttgaa tcaaaaatat
    12661 ataaaaaatt gacatataga catatttaga ccatcttttt ttaggatatg ccccacaatt
    12721 tcccttaaaa aaatcaaaac ccttatttct agctatcagt cgattagcac aatgttccgg
    12781 acaaaatatc catagacata aaaaaaagac ctgccagaca ggtctttctt atcttccatc
    12841 acctcaaaag tgatatttaa ttactcctat ttttactcca caaaaaatca atgggtcaaa
    12901 tatcccagct tggtagaaat tacaagggcg tatttcataa cgattttacc cttactttca
    12961 aattgcgatg caggaagtct accgtaagga gccgtaatcc aaagtgccgc tacgggataa
    13021 ccgtactcat tgaatatggg agcacccaca caattaatcc cttcaatatc ctcgctatta
    13081 tctaccgcat atcctttttc ccgaacttct ttcaacaccc tcttaaactg tttctttgat
    13141 gtaatggtat tttcggtaaa cttggtcaga ggttttccgt tcaaggccat atcagcttct
    13201 tccgagggaa tatgggccaa tatacattta ccccctaccg aactatgcag atcaaattgg
    13261 gtaccaggtt caacaaatag tttgaccggg taggaagagg gcacctgttc caaaatagtt
    13321 cccgtagagc ctagaagcac ccctagcatg accgattcct tgagctcatc gcgcaatgcg
    13381 cgcatgacgt ctatagaatg ctctacaata ctctgttcgt tcatagaaga aataccaagg
    13441 gtcagcattt ttctagaaag ggttattttt ttggtggttt cgttcttctg aagataattc
    13501 ttgaatataa gggtactgac aatccgaaaa gcactggatt ttgtaatgga aagggtttct
    13561 gttatttccg caagggtcat acctttggtt cgagtagcca ataactcaat aatcgaaagc
    13621 cctctctcta agtttggaac attgtaagtg gactttaatt ctgccttcgg tttcttcatt
    13681 ttaaaatatt atatgcgcaa tttaacaatc ttgggcgtat tttcgcaaaa atttgcatag
    13741 gctcccatag agagactccc cttcgaccaa tcttctaaag ggacgaaatg tactcttcca
    13801 gattgaccga tctttccaat ttcatttttt tgcgcaatct atatcttgtt gtgtgcaccg
    13861 actcgaccga aatccccaaa agtctagcta tttccttact tgaaaagttt agtttgatca
    13921 aagcgcacaa cttttgatcc gcttgactta gcttagggta cattgtagtg atcttattat
    13981 aaaagtcttc gtttaccgcg gtaaagcgaa ggcggaattc ctcccaattg tcgcttcgat
    14041 tatgggaaat ggattttaaa acctgtttga cctctggttt atcggagatt ccttccgctt
    14101 cttttaacct ttccgctatt tccttcaaaa actcatcctt ttcgaccatt tgaagcgccg
    14161 atacggccaa ctctttattc ttcaagtcaa gaagttcctt ccccttcttg atttcgagct
    14221 gcttatttct ccgcaacatt tgcttttctg tcaaatgcct agttcgaatc gacctgaaat
    14281 aaataagacc aagtatagcc ataaaaacca aggagcccaa taaaataata cgctgcagca
    14341 tcgatatctt atcggcctgt tccaattttt ccaaacgctg tttccgcaca aggcgttcct
    14401 gtttttcctt ttctacccta aattcatcct ttatttccaa aagactctgg ttgattgccg
    14461 ttctactatc aaagaactgg gcgtcaatgg cttttgcctt cttttggttt ttataggcat
    14521 tttcatacgc cccgagactt tcatacaaat cggccagttt ctcatacacc aaggaagaga
    14581 aatcaacatg gctattgtat tcttccgaaa ttgccaaggc ttttttatag taaacttcgc
    14641 tttgtttata attggacata ttcgcataga cgtcacccat atcggaatac accaaaacca
    14701 gaaaagacgg cccgttcttt aaaaaccacg actctacttc ccccaacaac agcagggcct
    14761 cttcgttttt attatccaaa tacaattgaa aggcctgttc aaaatgtaaa taagccatag
    14821 gaagttgccc gtctatggga gcataattta caaaacaact atcaagatac accctggcca
    14881 agtggtgctg gtttaatatc ctatgggtag cgcttatcaa gtaataattt tcaaccagat
    14941 cagctttttg cagtttgccc ctgtcaatta acttgcgatt gatttcaagg gaggtctgca
    15001 aatactctag ggcctcgtct tttcgtttaa agaaactata aagtctacct agcccattat
    15061 agacagaggc ctttaaagca tcatcatcga cactatccgc caaaaaaagg gcataccaaa
    15121 gcgtatcgta tgattttgta taatcaacat gctgcccatt tatataaggc atctctaaaa
    15181 gagcctttac cgcattgacc gtgtcgcctt tttttaataa ttcttcatgt ttttcttgta
    15241 acaggaccaa tgcttcttca gggttgtcac gcctcaattt tcttgcttcc tcaaaaaacg
    15301 cttctccctg caggccttcc aaagtatcgg atagttgctg cgcatctaat ctacttagtg
    15361 gaacccaaca aaggataacc attataaaag aaaccaaatg tttgacctgc aaaaccatag
    15421 tttattctat atcctgattt aatttaaaca agttgtgata accgtcatta tttgcaacta
    15481 gaataaattt actgcctttt gtattcaacg cggctatatt acgaacatct ttatccgcaa
    15541 agaaacccga ttgcaaatta ggcaccaaaa cgaaagtgcc tttaccattt cctttcagaa
    15601 aagttccgat gccagcatct gagcgggtcg tttcaacttc agattgcttg ttattatcgg
    15661 ccaacaccaa gtatttgttc cacctctgta gtccatggtg ccatttacat caggtttata
    15721 aaaaactaaa attaaagtac caattcttaa aagcccaaca atgttaagaa aagccccata
    15781 agaacgcttc cctttaaaag gtttaaaaat cggtttttca taattgatta cattttaatt
    15841 gtgtagttct gagttaattt tttataacaa aacaataggc ctcaaattat caccttagaa
    15901 atattaccgt cccttttttg ctaaatcgcc caaaaaacct aaagtgaatg tatgtaatat
    15961 gtgttagttt tttgcacaat cgttcctaga caaaaactga acaacattaa ctttcaatgt
    16021 taaaataaaa actacatata actcataatc aacaatttaa caccaacaac aaacataaat
    16081 caaataatag acaactgaca attatgtagc tccagtgcaa gaatataaga aagtgaatat
    16141 ctactttgag taaagtatat ttagggcagt cccttgcatt ttaagatgaa gaaaatccgt
    16201 tttattagca tatttcatct aaagaatagg caagcgcaca tacgatttga ttcttatcca
    16261 aatggattta agaaactcgt ccatgtaaaa ttttaggact ttataaaaat ggattttcac
    16321 taaataaaca tttggctatt tgaagttctc cactagagcg taattccttc gccttcttaa
    16381 accttatttt tttataaaat ccttagaggc acactgcgga ctgtttttta atctcctaaa
    16441 aaagtagagc gactcgaatt catatcgaac gatggattac ctaccccata ggtatcgtct
    16501 gagatattct tctcaataag acgggaggtt cttttaagaa cctcccgttt atctgaattt
    16561 attgaagaaa cttccgaaat actttgtctc aacatttcct tttttcagtc gtattcatca
    16621 aaaaatttac tatctaatat tcaaacttat tacagtgatg aattttgtgt caaaactcca
    16681 ggctgaaggt ctaattgtac ttgtggtatt ggccaataat tgtttttacc agaagtaaaa
    16741 gaactgccag acaaccatgg tagcttattg tccttcaact cactagacaa ataattgttt
    16801 aaagtactat cagcaattcc ccatcttact agatcataga aacgcattcc ctcgagtgca
    16861 aattctaatc tcatttcgtg tcttacggcc atccgagcat attctacact aggaaaagaa
    16921 tcgtagagcc ctaatttata gtttgcggca ggttgtgagt aatctactat gataggattg
    16981 ccttcttcaa aagtagtatt tgatacttta cccattacaa tatcgtcagc tgccctctgt
    17041 cttatccgat ttaccaatgc aagagctgta ggtaaatcgt tttcctctac tgcgacttct
    17101 gctctccata ataatacgtg accaagcctt agcaacctga aattgttacc atttacccct
    17161 ggtgcccaat aaccggactc agttgaaatt acacctctat tacgcttata gaacatgttt
    17221 ttcttattca tgaaaggccc catgttcaat tgatcagaca tccaatcgct tccactcatt
    17281 ggcccccaat ctaaaaatgg gatacctctt cttccaatag tccaatcaac cctaggatct
    17341 aataaatgac tagtcggtgt aaattcttcc gtatctgaaa tttggtaatc ctctaaaaga
    17401 aggtcatcct gaaaactatc taacaatgga agaccattat cttctacttt atacgcattg
    17461 aaaaggtcaa aactaggtgc actataagag taacccagcc cttcaacatt tggatacaaa
    17521 gtctggtgat caacgccgga attatgtaca cctgccccat catttacaga atattgaatt
    17581 tcaaaaatac tctccccatt attttgatgt tcttcatcaa aattattgta aaaatgaggc
    17641 tctaactgaa aaggtccctc ttcaataatg ttatctagaa gtggttttgc ttccgcaaat
    17701 tcactctgaa acaaatgaac tcgtgctttt aatgcctttg ctgcccattg tgatgcccta
    17761 ccaacttctg atggtgccgg atctagatgg tcaatcccaa attgaagatc atcctctata
    17821 tcatcccaaa tctcccgatc gtttttaacc aaagctggat caacatcctc ggttatatag
    17881 ggtacttttt caaatttaat tcttagttga aaatgaaacc atgctcgtaa gaatttagct
    17941 tcggcttcaa tttggtcagc cttttctgtc gatattactt ctccggccgt tgctaaaaca
    18001 cgaagaacat cattagagcg cgctacccca tcaaaattaa caccccaggc accatttaac
    18061 cattcattat ctgaccttac ttcatatcgt tctatatcat taatttgcga ccaacctcca
    18121 ttggtatccc ctctatgcat atcatcagaa ggaacgtctc cccaaatcca attggttcca
    18181 gaagcctctc cttggccacc cttaggacta ccgttactag agtatccatc aattaatgaa
    18241 tatgcaccta taagcaatcc atttacccct tcctcattgg cgagggttaa ttcgtttacg
    18301 gtacccacag gctcctcatt aagaaaatca tcactacaag aggttatcaa aaccaaaatt
    18361 gagatggcaa aactatttgc gaaaaattta tttttcatct tttctatttt ttttaaaatt
    18421 ataatgagat attcaatccc aacatgatct gtttagatat tggccaagta cccacatcaa
    18481 cccctcggtc aatttcttgt ggaacgccat tttctctctt agcactaatt tcaggatcta
    18541 gccctgaata gttggtaaac gtgaataaat ttgagccaat tacatacaat ttcatatgtt
    18601 taaggtgcag cttttctatt atttcatcag gaagggtata ccccacctgt aggttttgaa
    18661 tacgtaaaaa agagccatcc tctatataaa gcgttgaagc attttgctca aatgcagttg
    18721 aagtagatgc tttaggtaaa gtagcatcat tattatcctc taggtaagga cttccccatg
    18781 agttatataa tctttccgga ctatttggac cttcaaaaag tccgtattta gtaaatctac
    18841 ttgtgtaatt cgccaaatca ttccccacac ttgcataaag tgttgccgaa acatcaatat
    18901 ttccaaatcc catacctaaa cggaaacctg ccgtaaaatc cggatggggg ttaccgatat
    18961 aggtacgatc ttctggagta atctctccat cgccatccac atctctaatt ttcaaattac
    19021 ccggttcatt ataagtacca ttagtagcat gtgcatccgc ttcactttgc gtttgaaaaa
    19081 taccatcaac tacgtaacca taatactcag gaaaagatct tcccacttcc gttctagtat
    19141 agatttgctg acgtgttgga aatccctgta taaactcatt cccattatca gaaagtttag
    19201 tgacctcatt tttataagcc gtaaacgtgg tcgacatatt aagggtaaac caatcatcca
    19261 atgctgaagt acggtacgaa agcgatatgt ctaccccctt atttgacatc ccccctatat
    19321 ttaccgaagg ttcaataaca tcacccacaa cttgcggaat tgccacggta aacaacatat
    19381 cctcagtatc ctttttccaa aaatccacag acaaatccaa ttttctaaat aaagtggcat
    19441 caaaagcaat attagtagac gtggtagttt cccatttagc atttggatta ccaattgcca
    19501 aagactgata tccttgagta atcgtattat ccgatccacc aatagcataa taagatgatc
    19561 cagggtccga ctcatatgta gtgaaactat tgtaattccc gatttgatca ttacccgatt
    19621 gcccccaact tgctctaaat tttaaataac tgagccagtc ttcagtacca ctcaaaaaat
    19681 tctcttttga cgccacccaa cctaaagatg cagctggaaa gattccatat ctattttcag
    19741 caccaaaacg agatgaacca tcacgccgta cagtcaagtc taaaatatac ttttttgcat
    19801 agtcgtaatt aattctagag aaataggaaa ataatgtaga cgcaaatccg ctaccattgt
    19861 tcaactggtt actagcacct gcatccaaca ctaagaaatt aatatcatta gaaaggtact
    19921 cctgtctaga tgcagaaata gttctatatg ctattcttga agattccata ccaaaaagaa
    19981 aatctacgct atggtcttct ccaaatgtgt ttttatagtt aaatgtattt gtccagttcc
    20041 aacgtgtttc attggcacta ctttcagtaa gagtggtaga tttagcaccg gtattttgtt
    20101 cccaagcagc aaaagaaggt gagaaattat taaacaaatc cacatcataa cccaacaaag
    20161 tcttgatcgt tacattttta ataggttcaa tcgccgcata aacatttccg gtaatgttgt
    20221 attttttatt actattatct ttaagcctat ctaaaaaacc tacaggatta ggaccatcat
    20281 taatattagc tccaccaaca acaccaccag cccagtttcc agcaatatcc ctaacaggaa
    20341 ccaatggact agctaaatag atttgattaa ataagttatt atgtgaattg ttaccgcccc
    20401 agcctttggt ttctccataa gtaaccccta aacgctgacc aatattcaac cattcaaaag
    20461 gatttgaatc aacgttagac cggaaagaaa ttctattaaa tgacgtattc ttaagtatac
    20521 cattctcttc aagaaaacct gcctgaaagg aataggaggt tttttcagag ccccccatca
    20581 atgacaaatc gtaattctga atcaaaccgt gttgatatat ttcatctaac caatccgttc
    20641 cctgcggatt tgatttggta atgggataat ttacttggtc atacaaagca ggattaacat
    20701 tggccgcacc tgaaacccct ccgtttggat acaagtactc attaagaacc ggtgttgccc
    20761 cacttccgaa atgggcatta gtttgtggca gaccatcgtt ctctaactcc aaccacaaca
    20821 tctctcctat caattgagga tccatcatga tatcatactt ggcatccgaa aaactcatgc
    20881 ccgttttcac actgatatcc agcttcactg cttgattctt cttaccggtt ttcgtggtaa
    20941 ccaaaataac accattggca cctcttgcac cataaatagc cgtagaggat gcatctttta
    21001 agatttgaat ggattcaatt tgtcctggag gaggcgattg cgcaccttga acaccatcaa
    21061 caacccatag tggaccgtta ttatttatgg ttcccatacc ccgtattcgt acctcggcat
    21121 cacctcctgg tgtagaggca ctaattgtcg tcacccccga agcactaccc tgaagggcct
    21181 tacttacatt tgaaacgggt tgttgttcaa gataattttt atcaaccgta gctactgcac
    21241 tggtaagggc atccttactt cttgtaccat aaccgataac cacaacttca tctaattgtt
    21301 gttgatcggt aagtaaagtg atattcaatg tggtctgatt acccacttta atgttttggg
    21361 taccataacc aagataggaa ataacaagta catcctctgg ggatgcttca atactaaaat
    21421 ttccgtcaaa atcggtggtt acaccgttag aggtcccttt gatcaaaatg ttgacccccg
    21481 gaataggccc ttgctcatcg gaaacggttc ccgtcaccat ggtttgcgcc tgaatatggc
    21541 ttgaaaacaa gaaaaaaatg gcaagaaaaa ctcccatttt caaaaatttt aaaagcttca
    21601 t
//
