LOCUS       FP476056                5910 bp    DNA     linear   BCT 27-MAR-2015
DEFINITION  Zobellia galactanivorans strain DsiJT chromosome, complete genome.
ACCESSION   FP476056
VERSION     FP476056.1
DBLINK      BioProject: PRJEB8976
            BioSample: SAMEA3315864
KEYWORDS    .
SOURCE      Zobellia galactanivorans
  ORGANISM  Zobellia galactanivorans
            Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Zobellia.
REFERENCE   1
  AUTHORS   Thomas,F., Barbeyron,T., Tonon,T., Genicot,S., Czjzek,M. and
            Michel,G.
  TITLE     Characterization of the first alginolytic operons in a marine
            bacterium: from their emergence in marine Flavobacteriia to their
            independent transfers to marine Proteobacteria and human gut
            Bacteroides
  JOURNAL   Environ. Microbiol. 14 (9), 2379-2394 (2012)
   PUBMED   22513138
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Genoscope -,C.E.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Genoscope - Centre National de Sequencage :
            BP 191 91006 EVRY cedex - FRANCE (E-mail : seqref@genoscope.cns.fr -
            Web : www.genoscope.cns.fr)
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Zobellia galactanivorans"
                     /mol_type="genomic DNA"
                     /strain="DsiJT"
                     /db_xref="taxon:63186"
     gene            1..2352
                     /gene="alyA4"
                     /locus_tag="ZOBELLIA_4130"
     CDS             1..2352
                     /gene="alyA4"
                     /locus_tag="ZOBELLIA_4130"
                     /EC_number="4.2.2.3"
                     /function="Alginate lyase"
                     /note="Alginate lyase catalyzes the eliminative cleavage of
                     polysaccharides containing beta-D-mannuronate residues to
                     give oligosaccharides with
                     4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at
                     their ends. It belongs to the family 6 of the
                     polysaccharide lyases. Features an uncleaved signal
                     peptide.; High confidence in function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="PL6|PL6_1"
                     /protein_id="CAZ98265.1"
                     /db_xref="GOA:G0L7T1"
                     /db_xref="InterPro:IPR006626"
                     /db_xref="InterPro:IPR011050"
                     /db_xref="InterPro:IPR012334"
                     /db_xref="UniProtKB/TrEMBL:G0L7T1"
                     /translation="MTKQILSVSNMTKASLLVLSVVLSLFSFIENPNDKGIYVKNSTEL
                     NEAIDQANPGDEIIMANGDWNDVQIRFLGYGKEGQPITLKAETPGKVFIGGKSDLKLGG
                     EYLVVNGLYFINGASPSNAVIEFAINEDTVANHSRITNSVILDFNKAQRNETDLWVLFK
                     GRHNQLDHCYIAGKSNRGPTVRIDLAGNQNIKNYHKINNNYFGPRPPKGGPSAETIQLG
                     NSYTSMTPSHTLVAHNFFDRCNGEVEVISSKSNFNEFRNNVFYKSEGSLVTRHGNYCII
                     DGNYFIGDTNSEQIGGVRLIGTGHWVTNNYFYNLKGKTFRSPLAVMNGIPKSPLNRYIQ
                     VTDVVVVHNTWVNCTSPLQFGVGSNVDQKDILPASEIRSERPIRTIVANNLIYSDKGDK
                     QPVVAHDSLDGIRFRNNLINNQGLSFRKLPGVETKDFSLSELEENVLIPANDLSGSALY
                     KGFEFDQITTDLFGNSRAEQNAVGAIVKAPTQKPNLIDISQYGPDWFPLNSDKKDVVKT
                     YSVRSTSQLISAVQKAKQGDTIRLTASKYKLKTPLKIDKHLTLQSADIHKKAIIHYNGA
                     AKTPAFEMNPKGQLTLKGLILKGEKAQYAFASLKNNMSSLYNLTVVDSEISGFDYVLKA
                     YKYSFSEYIQLKSTVITDCANGLELSEETDDKGEYNAENIYIDNCRFKGVGKNVIDYYR
                     GGYDESTVGGNLVVTNSSFTQCGAKEESEILLNTYGIINVNLSGNKFRDNQVKYVARLW
                     GAKNNMHSDNEITNSGELIVEENLPLKLMY"
     gene            2369..3427
                     /gene="alyA5"
                     /locus_tag="ZOBELLIA_4131"
     CDS             2369..3427
                     /gene="alyA5"
                     /locus_tag="ZOBELLIA_4131"
                     /EC_number="4.2.2.3"
                     /function="Alginate lyase activity"
                     /note="Alginate lyase catalyzes the eliminative cleavage of
                     polysaccharides containing beta-D-mannuronate residues to
                     give oligosaccharides with
                     4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at
                     their ends; Belongs to the family 7 of the polysaccharide
                     lyases (PL7); Contains a putative lipoprotein signal
                     peptide cleaved between the residues 16 and 17; Possibly
                     localized in the outer membrane; High confidence in
                     function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="PL7_5|PL7"
                     /protein_id="CAZ98266.1"
                     /db_xref="GOA:G0L2Y1"
                     /db_xref="InterPro:IPR013320"
                     /db_xref="InterPro:IPR014895"
                     /db_xref="PDB:4BE3"
                     /db_xref="UniProtKB/TrEMBL:G0L2Y1"
                     /translation="MKKTVLATVTVFVLFACKDKPKATTDSASESAVAMKNTTKYPSEL
                     IPQMDEWKILLGDGTHKEDLVNYAKDDFFYVEHENETDWVVFKTPNSGITSRTSSNTRT
                     ELGQKKHWIPETGGKLNATLKVQHVSTSGDARVAASYSVVVGQIHSDEGHENEPIKIFY
                     KKFPGHTKGSVFWNYEINTKGDNSKRWDYSTAVWGYDMSVVGPTATSYPEEPEDGIALG
                     EEFSYEINVYEGIMYLTFSSEGHKTIKFTKNLLKSNFTKKSDIPQQIKTLYASIGRDGI
                     ERENAYAGEIQYFKLGAYNQTNGKSPEDNLVWSTGADVYDGDIAKQYANGSYAEVWFKE
                     ATLGSGSAPEAQ"
     gene            3577..5910
                     /gene="alyA6"
                     /locus_tag="ZOBELLIA_4132"
     CDS             3577..5910
                     /gene="alyA6"
                     /locus_tag="ZOBELLIA_4132"
                     /EC_number="4.2.2.3"
                     /function="Alginate lyase"
                     /note="Alginate lyase catalyzes the eliminative cleavage of
                     polysaccharides containing beta-D-mannuronate residues to
                     give oligosaccharides with
                     4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at
                     their ends. It belongs to the family 6 of the
                     polysaccharide lyases. Features a signal peptide cleaved
                     between residues 22 and 23. Localized in the periplasm.;
                     High confidence in function and specificity"
                     /codon_start=1
                     /transl_table=11
                     /product="PL6|PL6_1"
                     /protein_id="CAZ98267.1"
                     /db_xref="GOA:G0L7T2"
                     /db_xref="InterPro:IPR006626"
                     /db_xref="InterPro:IPR011050"
                     /db_xref="InterPro:IPR012334"
                     /db_xref="UniProtKB/TrEMBL:G0L7T2"
                     /translation="MRHLHNFFSFIFLLGTFSFVTAQQKNTVKNVAEFNEALRTVQPGD
                     SIVLANGIWKDSELLFEAEGTAEKPITLTVEEKGKVTLEGESNLRMAGNHLIVKGLIFK
                     NGHTPTNAVISFRKDREKMANHSRLTECVIDNFNNPERQVQDYWITIYGKNNRIDHNHI
                     VGKKNLGVTMIVGLDTEGSRANNHKIDHNYFGPRPTYGNNGGETLRIGTSHHAMENSNT
                     LVESNYFDRCNGEHEIISNKSCQNTFKYNTFFECTGTLTMRHGNETLVDGNVFIGNGKP
                     STGGVRVINESQTVINNYHIGLTGYRFRGAFVMMNGVPNSPPNRYVPVIDSKVNNNTFV
                     NCENILFGAGSDEERSQAPQTSEFSRNIIYNDTKKDIFTINDDISGISFKNNILGKNSE
                     TTIEKGFVNADIQLVKNGQGFLIPTSKKIKHKVTISPNIATKENTGVTWYSKEDKTVAL
                     NSGKTIKVKAGINTLYDEVKKSEAGDIMLLEEGGTYLLTKAVKINHPLSFKTEGKEKAK
                     IFFERMMAFEIQDGGSLSLENISFDGAKSPDYAGNSVISTSKNSMIENYKLFIDKCEFK
                     DMVVNHSFDVLRVSKGTFADTISIKNSTFRKITGSITALDTETDDIGAYNVEYFIMKNN
                     IISELQGAALRLYRGGKDESTFGPFLEVDHNVFDKVGFGKKNKYKSAISLYGVQEIDIK
                     NNIFRDSKAIQMHLVVGEPIVKVRNNALSNSEKLVVTGDQEYFVENQWDLDPKFSDESS
                     YTLSADSPLKGKATDGGDLGVISQ"
ORIGIN
        1 atgacaaaac aaatcctatc agtaagtaac atgacaaaag ccagtttatt agtactaagt
       61 gtggttcttt ccttgttttc ctttatcgaa aacccaaacg ataaagggat ttacgtaaag
      121 aatagcacag aattgaatga agccatagat caggcaaatc ctggagacga aattatcatg
      181 gccaatggag attggaacga tgttcaaatc cgatttttgg gatatggaaa ggaagggcag
      241 cccataaccc tcaaggcgga aacacctggc aaagtcttta ttggcggaaa atcggaccta
      301 aaattaggcg gggaatacct tgttgtcaac ggtttgtact tcatcaatgg tgcatcgcct
      361 tctaacgcgg tcattgaatt tgccattaac gaagatacag tggccaatca ctcccgaatt
      421 accaattccg tcatcttaga tttcaataag gcccaaagaa acgagaccga cctttgggta
      481 ttgttcaagg gaaggcataa ccagttggat cattgctaca tcgccggaaa gtccaataga
      541 ggccccacgg tcagaattga tttggccgga aaccagaaca taaagaatta ccacaaaatc
      601 aacaacaact attttggccc aagaccccca aaaggcgggc ctagtgccga aaccatacaa
      661 ctagggaaca gttatacttc catgaccccg agccacacct tggtagcgca taatttcttc
      721 gataggtgca atggagaggt ggaagtcatt tcaagcaaat caaattttaa tgagttcagg
      781 aacaatgtct tttataaaag cgagggctca ttggtaacaa gacacggcaa ttactgtatc
      841 atagacggta actattttat aggagataca aattccgaac agatcggcgg cgttaggttg
      901 atcggaacag gtcattgggt taccaacaat tatttctata acctaaaagg aaaaaccttt
      961 aggagtccac ttgccgttat gaacggtatt ccaaaatcac ccctcaatag atatatacag
     1021 gttacggatg tggtagtggt ccacaacaca tgggtcaatt gcacctcgcc tttgcagttc
     1081 ggggtcggtt ccaatgtgga tcaaaaagac attcttcccg cctcggaaat caggtcggag
     1141 cgcccgattc gaacgatagt ggcaaataac cttatctata gtgacaaggg agataaacag
     1201 cctgtggttg cccacgattc cttagatgga atacgcttta gaaacaacct cataaataat
     1261 caaggacttt cattccgaaa gcttccaggg gtggaaacca aagatttttc cctttccgaa
     1321 ttagaagaaa atgtcctgat tcctgcaaat gacctgtcgg gttcagcgct atacaaaggt
     1381 tttgaatttg atcagattac gaccgacctt tttgggaact ccagggcgga acaaaacgcg
     1441 gtcggtgcca ttgttaaagc cccgacccaa aagccgaatc ttatagacat ctcccaatac
     1501 ggtccggatt ggtttccttt aaattccgat aaaaaggatg tagtcaaaac ctattccgta
     1561 cgttcaacct cccaattgat cagtgcggtg caaaaagcaa aacaggggga taccatacgt
     1621 ttgactgcta gcaaatacaa gcttaaaacc ccgttgaaaa tcgataaaca cttgacccta
     1681 cagtcggccg atatccataa gaaggctatc attcattaca acggtgccgc aaagaccccg
     1741 gcatttgaaa tgaaccccaa aggacaactg accctaaaag ggcttatcct taaaggagaa
     1801 aaagcccaat atgcatttgc cagtttgaaa aacaatatgt ccagtctcta taacttaacg
     1861 gtcgtagatt ctgaaatatc gggttttgac tatgtgctca aggcctacaa atattccttc
     1921 tccgaataca ttcaactgaa atctaccgta attaccgatt gtgccaatgg tctggagctg
     1981 tcggaagaga ccgacgacaa aggcgaatac aatgccgaaa acatatatat tgacaattgc
     2041 cgttttaaag gcgttggcaa aaacgtaatc gattattacc gagggggcta cgacgaatcg
     2101 accgtgggag gcaatctggt cgtgaccaac agcagcttca ctcagtgcgg ggcaaaggaa
     2161 gaaagcgaaa ttttactcaa cacctatggt attataaacg taaacctttc cggaaacaaa
     2221 ttccgagaca accaagtgaa gtatgtggcc cgcctttggg gagcaaagaa caatatgcat
     2281 tccgacaatg aaataacaaa ttcaggggag ctgattgttg aagaaaacct cccactcaaa
     2341 ttgatgtatt aaaccataca acctaaaaat gaaaaaaaca gtattagcta cagttaccgt
     2401 attcgtcctt ttcgcctgta aagacaaacc taaggccact acggacagtg catcggaatc
     2461 ggccgttgca atgaaaaaca ccactaaata cccaagtgag cttatccctc aaatggatga
     2521 atggaaaatt ctcttaggcg atggcaccca taaagaagac cttgtgaatt atgcgaagga
     2581 tgactttttt tatgttgaac atgaaaatga aacagattgg gtagtattca aaacaccgaa
     2641 ctcgggcatt acctcaagga cctcaagtaa taccagaacg gaattagggc agaaaaaaca
     2701 ctggattccc gaaacaggag ggaagttgaa tgcaacctta aaggttcaac acgtctccac
     2761 ttcgggcgat gctagggttg ccgcgtcata ctctgtcgtg gtcggccaaa ttcacagcga
     2821 tgaaggacac gaaaacgaac cgataaaaat cttttacaaa aaatttccag gccatacaaa
     2881 aggctccgtt ttttggaatt acgagattaa caccaagggt gataattcaa agcgatggga
     2941 ttactccacg gcagtttggg gatacgatat gtcggtcgtt ggcccaacgg ctacctcata
     3001 ccccgaagaa ccggaagacg gtatagcctt gggggaggag tttagttatg aaatcaacgt
     3061 ttatgaaggt atcatgtacc tcactttttc tagtgaaggc cacaagacca ttaaattcac
     3121 caaaaaccta ttaaaatcca attttaccaa gaagtccgat attcctcaac agataaagac
     3181 gctgtatgct tcaataggtc gtgacggtat cgaacgtgaa aatgcctatg ccggggaaat
     3241 acaatacttt aagctaggag cctacaacca aaccaacgga aaatccccag aggataatct
     3301 ggtatggagt acaggggccg acgtttacga tggtgacatt gccaagcaat atgcaaatgg
     3361 aagctatgcc gaggtatggt tcaaagaagc gacgcttgga agtggaagcg ccccagaagc
     3421 ccagtgatac tgcggaaagt aacccccctt ctattggggt gatatgattt aagggacccg
     3481 ttttatatat taaacaccga ttttgtgtta aatatttgtc ggttctgtaa taatatgtat
     3541 ttttggtaaa ccaaactggt taaccaattt agaactatga gacacttaca caattttttt
     3601 tcctttattt ttcttctcgg taccttctcg tttgtaacgg cccaacaaaa aaatacggtc
     3661 aagaacgtag ccgagtttaa tgaagcgctc cgaacggtac aaccgggcga tagtattgtt
     3721 cttgccaatg gtatatggaa agactccgaa cttctgtttg aagcagaagg gacggcagaa
     3781 aagcccatta cccttacggt agaagaaaaa ggaaaggtga ccttggaagg cgaatcgaac
     3841 ctaaggatgg ccgggaatca tttgattgta aaaggtctga tctttaaaaa tggccataca
     3901 ccgaccaatg cagtgatttc ctttaggaag gaccgggaga aaatggccaa tcatagccgc
     3961 ttgaccgaat gtgttattga caacttcaac aaccccgagc gtcaggtaca ggattactgg
     4021 ataaccatct atggtaaaaa caaccgtatt gaccacaacc atatcgtagg gaaaaagaat
     4081 ttgggagtca ccatgatcgt cggcctcgac acagaaggta gcagggcgaa caaccataaa
     4141 atagaccaca attatttcgg gccacgcccc acttatggaa ataatggtgg cgaaacctta
     4201 cgaattggga cgagccatca tgccatggaa aattccaaca ccttggtaga atccaattac
     4261 tttgaccgat gcaatggcga acatgagatc atctcgaaca agtcttgcca gaacactttt
     4321 aaatacaata ccttctttga gtgtacggga accttaacca tgcgtcatgg taatgaaacc
     4381 ttggttgacg gcaatgtctt tattggtaac ggtaagccga gtacaggagg ggttagggta
     4441 attaacgaaa gccagacggt cattaacaat taccatatag gattaaccgg atatcgtttt
     4501 cgaggggctt tcgttatgat gaacggcgta cccaattccc cgccaaaccg ctacgttccg
     4561 gtaatcgact caaaagtaaa caacaacact tttgtaaatt gtgaaaatat cctttttggc
     4621 gcgggtagcg atgaagaaag gagtcaagct ccccaaactt ccgaattttc aaggaatatc
     4681 atttataacg ataccaagaa agatatcttc accattaacg atgatattag cggaattagc
     4741 tttaaaaaca atatcctcgg gaagaattct gaaaccacta ttgaaaaagg ctttgtaaac
     4801 gccgacattc aattggtaaa aaacgggcag ggcttcttga ttcctacctc aaaaaaaata
     4861 aagcataagg taaccataag tccgaatatc gccaccaagg aaaatacagg ggtaacttgg
     4921 tattccaagg aagataaaac cgttgcattg aattctggta aaacgatcaa ggtaaaagcg
     4981 gggatcaaca ctttatatga tgaagtaaag aagtccgaag caggtgatat catgcttttg
     5041 gaagaaggcg gcacctattt gttgaccaag gccgtgaaaa taaaccatcc cctttccttt
     5101 aaaacggagg gtaaagaaaa ggctaagatc ttctttgaaa gaatgatggc ctttgagatc
     5161 caagatggag gaagcctgtc tttggagaac ataagctttg acggggcgaa atccccggat
     5221 tacgcaggta attccgttat aagcaccagt aaaaactcga tgatagaaaa ctataagttg
     5281 tttattgata aatgtgagtt taaagatatg gtcgtcaacc attcttttga tgtgttaagg
     5341 gtttcaaagg gcacttttgc cgataccatc agtatcaaaa attcgacctt cagaaaaatt
     5401 acgggaagca ttactgcact agacaccgaa accgatgaca taggggccta taatgtagag
     5461 tacttcatca tgaagaacaa tatcattagc gaactacaag gcgcggcatt gcggttgtat
     5521 agaggcggta aggacgaaag taccttcggg ccttttctag aagtcgacca caatgtattc
     5581 gataaggtcg ggttcggcaa gaaaaataaa tacaagtcgg ccatctccct ttacggtgtg
     5641 caggaaatcg atatcaaaaa caatattttt agggacagta aggcgattca aatgcacttg
     5701 gtagttggtg aacctattgt aaaagtccgg aataacgcct tgagcaattc cgagaaactc
     5761 gtggtaacgg gagaccagga atacttcgta gaaaaccaat gggacctaga ccctaaattc
     5821 tccgatgaat catcatatac cttaagtgca gattctccct taaagggaaa agctaccgat
     5881 gggggcgatt tgggcgtaat ttcccaataa
//
