LOCUS       HG738867                9337 bp    DNA     linear   BCT 27-FEB-2015
DEFINITION  Escherichia coli str. K-12 substr. MC4100 complete genome.
ACCESSION   HG738867
VERSION     HG738867.1
DBLINK      BioProject: PRJEB4621
            BioSample: SAMEA3138816
KEYWORDS    complete genome.
SOURCE      Escherichia coli str. K-12 substr. MC4100
  ORGANISM  Escherichia coli str. K-12 substr. MC4100
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Laehnemann,D., Pena-Miller,R., Rosenstiel,P., Beardmore,R.,
            Jansen,G. and Schulenburg,H.
  TITLE     Genomics of rapid adaptation to antibiotics: convergent evolution
            and scalable sequence amplification
  JOURNAL   Genome Biol Evol 6 (6), 1287-1301 (2014)
   PUBMED   24850796
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Laehnemann,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2013) Evolutionary Ecology and Genetics,
            University of Kiel, Am Botanischen Garten 1-9, 24118 Kiel, GERMANY
COMMENT     The annotation of this whole genome reference is derived from the
            Escherichia coli K-12 strain MC4100 substrain BW2952 reference
            (accession NC_012759) by whole genome Illumina paired-end
            sequencing, read mapping, variant calling, and reference adaptation
            by application of variants and manual curation of further
            annotation changes.
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Escherichia coli str. K-12 substr. MC4100"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MC4100"
                     /db_xref="taxon:1403831"
     gene            1..2619
                     /gene="bcsA"
                     /locus_tag="BN896_3222"
     CDS             1..2619
                     /gene="bcsA"
                     /locus_tag="BN896_3222"
                     /note="polymerizes uridine 5'-diphosphate glucose to
                     cellulose; acts with BcsB, BcsZ and BcsC in cellulose
                     biosynthesis"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="CDJ73343.1"
                     /db_xref="EnsemblGenomes-Gn:BN896_3222"
                     /db_xref="EnsemblGenomes-Tr:CDJ73343"
                     /db_xref="GOA:U6N9W8"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="InterPro:IPR003919"
                     /db_xref="InterPro:IPR009875"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:U6N9W8"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILAW
                     IFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPRRR
                     AFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTSTL
                     NWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFVPT
                     YNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHEHAK
                     AGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSPDPFE
                     RNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIAVETVTEDA
                     HTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPLTGKGLK
                     FAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPHMIHASLT
                     NSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVEEEYVDWVI
                     SRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGAVAVSVESKQ
                     VRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQKVNLLLKRGQ
                     QEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQDSYPEDKPLESL
                     LDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRPERSETAQPSDQALAQ
                     Q"
     misc_feature    43..2595
                     /gene="bcsA"
                     /locus_tag="BN896_3222"
                     /note="cellulose synthase catalytic subunit; Provisional;
                     Region: bcsA; PRK11498"
     misc_feature    550..1032
                     /gene="bcsA"
                     /locus_tag="BN896_3222"
                     /note="Glycosyltransferase family A (GT-A) includes diverse
                     families of glycosyl transferases with a common GT-A type
                     structural fold; Region: Glyco_tranf_GTA_type; cl11394"
     misc_feature    817..1515
                     /gene="bcsA"
                     /locus_tag="BN896_3222"
                     /note="CESA_CelA_like are involved in the elongation of the
                     glucan chain of cellulose; Region: CESA_CelA_like; cd06421"
     misc_feature    1078..1086
                     /gene="bcsA"
                     /locus_tag="BN896_3222"
                     /note="DXD motif; other site"
     misc_feature    2080..2373
                     /gene="bcsA"
                     /locus_tag="BN896_3222"
                     /note="PilZ domain; Region: PilZ; cl01260"
     gene            2630..4969
                     /gene="bcsB"
                     /locus_tag="BN896_3221"
     CDS             2630..4969
                     /gene="bcsB"
                     /locus_tag="BN896_3221"
                     /note="binds the cellulose synthase activator, bis-(3'-5')
                     cyclic diguanylic acid (c-di-GMP)"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P37652|4.D.3.1.6"
                     /protein_id="CDJ73344.1"
                     /db_xref="EnsemblGenomes-Gn:BN896_3221"
                     /db_xref="EnsemblGenomes-Tr:CDJ73344"
                     /db_xref="GOA:U6NDC3"
                     /db_xref="InterPro:IPR003920"
                     /db_xref="InterPro:IPR018513"
                     /db_xref="UniProtKB/TrEMBL:U6NDC3"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTEQ
                     NPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGMRS
                     DEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPINPLF
                     ISDFNRVRLEFVGHYQDVCEKPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPVPFFD
                     PSDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRNAIVFA
                     TNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQGNILFR
                     GESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAINVSLNLP
                     PDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEANRLLLRIPV
                     LQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQNHVVIGDDST
                     IDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMETLLNTVGFIGA
                     QTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQATESWVKTPMRQT
                     PFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLADSPRGYEMLNDA
                     VNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYALANHPILLAVLAAI
                     SVILLAWVLWRLLRIISRRRLNPDNE"
     misc_feature    2630..4861
                     /gene="bcsB"
                     /locus_tag="BN896_3221"
                     /note="cellulose synthase regulator protein; Provisional;
                     Region: PRK11114"
     misc_feature    2834..4672
                     /gene="bcsB"
                     /locus_tag="BN896_3221"
                     /note="Bacterial cellulose synthase subunit; Region: BcsB;
                     pfam03170"
     gene            4976..6082
                     /gene="bcsZ"
                     /locus_tag="BN896_3220"
     CDS             4976..6082
                     /gene="bcsZ"
                     /locus_tag="BN896_3220"
                     /note="catalyzes the hydrolysis of 1,4-beta-D-glucosidic
                     linkages in cellulose, lichenin and cereal beta-D-glucans"
                     /codon_start=1
                     /transl_table=11
                     /product="GH8"
                     /protein_id="CDJ73345.1"
                     /db_xref="EnsemblGenomes-Gn:BN896_3220"
                     /db_xref="EnsemblGenomes-Tr:CDJ73345"
                     /db_xref="GOA:U6NF65"
                     /db_xref="InterPro:IPR002037"
                     /db_xref="InterPro:IPR008928"
                     /db_xref="InterPro:IPR012341"
                     /db_xref="InterPro:IPR019834"
                     /db_xref="UniProtKB/TrEMBL:U6NF65"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIDP
                     SDARKITTSEGQSYGMFSALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKENS
                     KWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSML
                     LPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWV
                     RYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYP
                     PEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNYVLTLFG
                     QGWDQHRFRFSTKGELLPDWGQECANSH"
     misc_feature    4976..6079
                     /gene="bcsZ"
                     /locus_tag="BN896_3220"
                     /note="Glycosyl hydrolases family 8; Region: Glyco_hydro_8;
                     cl01351"
     gene            6115..>9337
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
     CDS             6115..>9337
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
                     /note="cellulose is produced by the multicellular
                     morphotypes of E. coli and Salmonella typhimurium; the
                     cellulose biosynthesis genes bcsA, bcsB, bcsZ and bcsC are
                     constitutively transcribed, however, cellulose synthesis is
                     dependent on the expression of adrA"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P37650|1.B.55.3.1"
                     /protein_id="CDJ73346.1"
                     /db_xref="EnsemblGenomes-Gn:BN896_3219"
                     /db_xref="EnsemblGenomes-Tr:CDJ73346"
                     /db_xref="GOA:U6NHA4"
                     /db_xref="InterPro:IPR008410"
                     /db_xref="InterPro:IPR011990"
                     /db_xref="InterPro:IPR013026"
                     /db_xref="InterPro:IPR019734"
                     /db_xref="UniProtKB/TrEMBL:U6NHA4"
                     /translation="MPMVEAAPTAQQQLLEQVRLGEATHREDLVQQSLYRLELIDPNNP
                     DVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSRTTMLLSTPDGRQALQQARL
                     QATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIPARRGEAINQLKRINADAPGN
                     TGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGASKIWYGQIKDMPVSDASVSALK
                     KYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQGLAAVDSGMAGKAIPELQQAVRA
                     NPKDSEALGALGQAYSQKGDRANAVANLEKALALDPHSSNNDKWNSLLKVNRYWLAIQQ
                     GDAALKANNPDRAERLFQQARNVDNTDSYAVLGLGDVAMARKDYPAAERYYQQTLRMDS
                     GNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIERSLQNDRLAQQAEALENQG
                     KWAQAAALQRQRLALDPGSVWITYRLSQDLWQAGQRSQADTLMRNLAQQKSNDPEQVYA
                     YGLYLSGHDQDRAALAHINSLPRAQWNSNIQELVNRLQSDQVLETANRLRESGKEAEAE
                     AMLRQQPPSTRIDLTLADWAQQRRDYTAARAAYQNVLTREPANADAILGLTEVDIAAGD
                     KAAARSQLAKLPATDNASLNTQRRVALAQAQLGDTAAAQRTFNKLIPQAKSQPPSMESA
                     MVLRDGAKFEAQAGDPTQALETYKDAMVASGVTTTRPQDNDTFTRLTRNDEKDDWLKRG
                     VRSDAADLYRQQDLNVTLEHDYWGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFV
                     NMNVGSFSTNADGKWDDNWGTCTLQDCSGNRSQSDSGASVAVGWRNDVWSWDIGTTPMG
                     FNVVDVVGGISYSDDIGPLGYTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGV
                     GLSLSYDKGEANGVWASLSGDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNM
                     IWHYDKDLSGYSLGQGGYYSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMP
                     RYPLMNLIPTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDY
                     APSHFLLYVRYSAAGWQGDMDLPPQPLIPYADW"
     misc_feature    6115..>9337
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
                     /note="cellulose synthase subunit BcsC; Provisional;
                     Region: PRK11447"
     misc_feature    6973..7311
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
                     /note="Tetratricopeptide repeat domain; typically contains
                     34 amino acids
                     [WLF]-X(2)-[LIM]-[GAS]-X(2)-[YLF]-X(8)-[ASE]-X(3)-[FYL]-
                     X(2)-[ASL]-X(4)-[PKE] is the consensus sequence; found in a
                     variety of organisms including bacteria, cyanobacteria,
                     yeast, fungi; Region: TPR; cd00189"
     gene            complement(order(6973..7257,6991..7296))
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
     misc_feature    complement(order(6973..6978,6982..6987,6994..6999,
                     7090..7095,7135..7140,7147..7152,7228..7233,7240..7245,
                     7252..7257))
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
                     /note="binding surface"
     misc_feature    complement(order(6991..6993,7027..7029,7039..7041,
                     7048..7050,7135..7137,7171..7173,7183..7185,7192..7194,
                     7237..7239,7273..7275,7285..7287,7294..7296))
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
                     /note="TPR motif; other site"
     misc_feature    8464..>9337
                     /gene="bcsC"
                     /locus_tag="BN896_3219"
                     /note="Cellulose synthase operon protein C C-terminus
                     (BCSC_C); Region: BCSC_C; pfam05420"
ORIGIN
        1 atgagtatcc tgacccggtg gttgcttatc ccgccggtca acgcgcggct tatcgggcgt
       61 tatcgcgatt atcgtcgtca cggtgcgtcg gctttcagcg cgacgctcgg ctgtttctgg
      121 atgatcctgg cctggatttt tattccgctg gagcacccgc gctggcagcg tattcgcgca
      181 gaacataaaa acctgtatcc gcatatcaac gcctcgcgtc cgcgtccgct ggacccggtc
      241 cgttatctca ttcaaacatg ctggttattg atcggtgcat cgcgcaaaga aacgccgaaa
      301 ccgcgcaggc gggcattttc aggtctgcaa aatattcgtg gacgttacca tcaatggatg
      361 aacgagctgc ctgagcgcgt tagccataaa acacagcatc tggatgagaa aaaagagctc
      421 ggtcatttga gtgccggggc gcggcggttg atcctcggta tcatcgtcac cttctcgctg
      481 attctggcgt taatctgcgt tactcagccg tttaacccgc tggcgcagtt tatcttcctg
      541 atgctgctgt ggggggtagc gctgatcgta cggcggatgc cggggcgctt ctcggcgcta
      601 atgttgattg tgctgtcgct gaccgtttct tgccgttata tctggtggcg ttacacctct
      661 acgctgaact gggacgatcc ggtcagcctg gtgtgcgggc ttattctgct cttcgctgaa
      721 acgtacgcgt ggattgtgct ggtgctcggc tacttccagg tagtatggcc gctgaatcgt
      781 cagccggtgc cattgccgaa agatatgtcg ctgtggccgt cggtggatat ctttgtcccg
      841 acttacaacg aagatctcaa cgtggtgaaa aataccattt acgcctcgct gggtatcgac
      901 tggccgaaag ataagctgaa tatctggatc cttgatgacg gcggcaggga agagtttcgc
      961 cagtttgcgc aaaacgtggg ggtgaaatat atcgcccgca ccactcatga acatgcgaaa
     1021 gcaggcaaca tcaacaatgc gctgaaatat gccaaaggcg agttcgtgtc gattttcgac
     1081 tgcgaccacg taccaacgcg atcgttcttg caaatgacca tgggctggtt cctgaaagaa
     1141 aaacagctgg cgatgatgca gacgccgcac cacttcttct caccggaccc gtttgaacgc
     1201 aacctggggc gtttccgtaa aacgccgaac gaaggcacgc tgttctatgg tctggtgcag
     1261 gatggcaacg atatgtggga cgccactttc ttctgcggtt cctgtgcggt gattcgtcgt
     1321 aagccgctgg atgaaattgg cggcattgct gtcgaaaccg tgactgaaga tgcgcatact
     1381 tctctgcggt tgcaccgtcg tggctatacc tccgcgtata tgcgtattcc gcaggcggcg
     1441 gggctggcga ccgaaagtct gtcggcgcat atcggtcagc gtattcgctg ggcgcgcggg
     1501 atggtacaaa tcttccgtct cgataacccg ctcaccggta aagggctgaa gtttgctcag
     1561 cggctatgtt acgtcaacgc catgttccac ttcttgtcgg gcattccacg gctgatcttc
     1621 ctgactgcgc cgctggcgtt cctgctgctt catgcctaca tcatctatgc gccagcgttg
     1681 atgatcgccc tattcgtgct gccgcatatg atccatgcca gcctgaccaa ctccaagatc
     1741 cagggcaaat atcgccactc tttctggagt gaaatctacg aaacggtgct ggcgtggtat
     1801 atcgcaccac cgacgctggt ggcgctgatt aacccgcaca aaggcaaatt taacgtcacc
     1861 gccaaaggtg gactggtgga agaagagtac gtcgactggg tgatctcgcg gccctacatc
     1921 ttccttgtcc tgctcaacct ggtgggcgtt gcggtaggca tctggcgcta cttctatggc
     1981 ccgccaaccg agatgctcac cgtggtcgtc agtatggtgt gggtgttcta caacctgatt
     2041 gttcttggcg gcgcagttgc ggtatcggta gaaagcaaac aggtacgccg atcgcaccgc
     2101 gtggagatga cgatgcccgc ggcaattgcc cgcgaagatg gtcacctctt ctcgtgtacc
     2161 gttcaggatt tctccgacgg tggtttgggg atcaagatca acggtcaggc gcagattctg
     2221 gaagggcaga aagtgaatct gttgcttaaa cgcggtcagc aggaatacgt cttcccgacc
     2281 caggtggcgc gcgtgatggg taatgaagtt gggctgaaat taatgccgct caccacccag
     2341 caacatatcg attttgtgca gtgtacgttt gcccgtgcgg atacatgggc gctctggcag
     2401 gacagctacc cggaagataa gccgctggaa agtctgctgg atattctgaa gctcggcttc
     2461 cgtggctacc gccatctggc ggagtttgcg ccttcttcgg tgaagggcat attccgtgtg
     2521 ctgacttctc tggtttcctg ggttgtatcg tttattccgc gccgcccgga gcggagcgaa
     2581 acggcacaac catcggatca ggctttggct caacaatgat gataacgcga tgaaaagaaa
     2641 actattctgg atttgtgcag tggctatggg gatgagtgcg ttcccctctt tcatgacgca
     2701 ggcgacgcca gcaacgcaac cactgatcaa tgctgagcca gctgtagccg cccagacgga
     2761 acaaaatccg caggtggggc aagtgatgcc gggcgtgcag ggcgctgatg cgccagtcgt
     2821 ggcgcagaac ggtccttcgc gtgatgtgaa gctgaccttt gcgcaaattg caccgccgcc
     2881 gggcagcatg gtgctacgtg gcattaaccc gaacggcagc attgagtttg gtatgcgcag
     2941 cgatgaagtg gtgacgaagg cgatgctcaa cctcgaatac accccatcgc catcgttact
     3001 gcctgtccag tcgcagttaa aggtttatct caatgatgaa ctgatgggcg tgctgccagt
     3061 gaccaaagaa cagttgggta aaaaaacgct ggcgcaaatg cccattaacc cactgtttat
     3121 tagcgacttc aaccgtgtac ggctggagtt tgtcggccat tatcaggacg tgtgcgaaaa
     3181 accggccagc accacgcttt ggctggatgt tgggcggagc agtggactgg atctgaccta
     3241 tcagaccctg aatgtgaaga atgacctgtc acacttcccg gtgccattct ttgacccgag
     3301 cgataaccgc accaacacct tgccgatggt ctttgcgggt gcgccggatg ttgggctgca
     3361 acaagcctct gccattgtcg cctcgtggtt tggttcgcgt tctggctggc gtgggcagaa
     3421 cttcccggta ctctataacc aactgccgga tcgcaatgcc attgtctttg caaccaacga
     3481 caaacggccg gacttcctgc gcgatcatcc ggcggtaaaa gccccggtga ttgagatgat
     3541 taaccatccg cagaatcctt acgtcaaact gctggtggtg tttggtcgtg acgacaaaga
     3601 cctgttgcag gcagcgaaag gtatcgctca gggtaacatt ctgttccgtg gtgaaagcgt
     3661 ggtagtgaat gaagtgaaac cgctgctacc gcgtaagccg tacgatgcgc cgaactgggt
     3721 acgtaccgat cgtccggtca catttggcga actgaaaacc tatgaagaac agttacaatc
     3781 cagcggtctt gagccagcag cgattaacgt ttcgctaaac ctgccgccgg atctctacct
     3841 gatgcgcagt accggcattg atatggatat taattaccgc tacaccatgc cgccggtgaa
     3901 agacagttcg cggatggata tcagcctgaa taaccagttc ctgcaatcct tcaacctgag
     3961 cagcaaacag gaggcgaacc gcctgctgct gcggattccg gtattacaag gtttgctgga
     4021 tggcaaaaca gatgtctcta ttccggcgct gaaactgggc gcgaccaacc agctgcgctt
     4081 cgactttgag tatatgaacc cgatgccggg cggttcggtg gataactgta ttaccttcca
     4141 gccggtgcag aatcatgtgg tgattggtga cgactccacc atcgacttct cgaagtatta
     4201 ccacttcatc ccgatgccgg atctacgcgc ctttgctaac gcgggcttcc cattcagccg
     4261 gatggcggat ctgtcgcaaa ccatcaccgt gatgccgaaa gcgcctaacg aagcacagat
     4321 ggaaacgttg ctgaatactg ttggttttat cggcgcacag acgggcttcc cggcgattaa
     4381 tctgacggtg accgatgatg gcagcaccat tcagggcaaa gatgccgaca tcatgatcat
     4441 cggtggtatc ccggacaaac tgaaagacga taagcagatc gacctattgg tgcaggcgac
     4501 cgaaagctgg gtgaaaacac cgatgcgcca gaccccgttc cccggcattg tgccggacga
     4561 gagcgatcgc gcggcagaaa cccggtcaac gctgacctct tccggtgcga tggcggcggt
     4621 gattggcttc cagtcgccgt ataacgacca gcgcagcgtg attgcgctgt tggcagatag
     4681 cccacgcggt tatgaaatgc ttaacgatgc ggtgaacgat agcggcaaac gcgccaccat
     4741 gttcggttcg gtcgcggtga tccgcgagtc cggtatcaac agcctacgtg ttggcgacgt
     4801 ttattacgta ggtcatctgc cgtggttcga gcgcgtgtgg tatgcgctgg caaaccatcc
     4861 gattctgctg gcggtgctgg cggctatcag tgtgatattg ctggcatggg tactgtggcg
     4921 tctgctgcga attattagtc gtcgtcgtct taacccggat aacgagtaat tgaagatgaa
     4981 tgtgttgcgt agtggaatcg tgacgatgct gctgctggct gcctttagtg ttcaggcagc
     5041 ctgtacctgg cctgcctggg agcagtttaa aaaggattac atcagtcagg aagggcgcgt
     5101 catcgacccc agcgacgcgc gcaaaatcac cacctccgaa gggcaaagtt acggcatgtt
     5161 ctctgccctg gcggctaacg accgtgcagc tttcgataat attctcgact ggacgcagaa
     5221 caatctcgct cagggttctt taaaagaacg tttgcccgcc tggctgtggg gcaagaaaga
     5281 gaacagtaag tgggaagtgc tggacagcaa ttcggcctcc gatggtgatg tctggatggc
     5341 ctggtcgttg ctggaggcgg ggcgtttgtg gaaagagcag cgttataccg acatcggcag
     5401 cgcgttgcta aaacgtatcg cgcgggagga agtggtgacg gtgcctgggc tgggttccat
     5461 gttgttaccg ggcaaagtgg gttttgctga ggataacagc tggcgtttta accccagcta
     5521 cctgccgccg acgctggcgc agtatttcac ccgctttggc gcgccgtgga ccacgctgcg
     5581 cgaaaccaat caacgtttat tgctggaaac cgccccgaaa ggtttttcgc cagactgggt
     5641 gcgctatgag aaagacaaag gctggcagct aaaagccgaa aaaacattga tcagcagcta
     5701 cgacgctatc cgcgtttaca tgtgggtagg catgatgcct gacagcgatc cgcaaaaagc
     5761 gcggatgctc aaccggttta aaccgatggc gacattcact gagaaaaacg gttatccgcc
     5821 ggaaaaagtg gatgtggcta cggggaaagc gcagggtaaa ggaccagtcg gtttttctgc
     5881 cgccatgctg ccctttttac aaaaccgcga tgcgcaggcc gttcagcgcc agcgcgtggc
     5941 cgataacttt cccggcagcg atgcctatta caactatgtg ctgaccctgt ttggacaagg
     6001 ctgggatcaa caccgtttcc gcttctcgac aaaaggtgag ttattacctg actggggcca
     6061 ggaatgcgca aattcacact aaacatattc acgctttccc tcggtctggc cgtcatgccg
     6121 atggtcgagg cagcaccaac cgctcagcaa cagttgctgg agcaagttcg gttaggcgaa
     6181 gcgacccatc gtgaagatct ggtgcaacag tcgttatatc ggctggaact tattgatccg
     6241 aataacccgg acgtcgttgc cgcccgtttc cgttctttgt tacgtcaggg cgatattgat
     6301 ggcgcgcaaa aacagctcga tcggctgtcg cagttagcgc cgagttcaaa tgcgtataaa
     6361 tcgtcgcgga ctacgatgct actttccacg ccggatggtc gtcaggcact gcaacaggca
     6421 cgattgcagg cgacgaccgg tcatgcagaa gaagctgtgg cgagttacaa caaactgttc
     6481 aacggtgcgc cgccggaagg tgacattgct gtcgagtact ggagtacggt ggcgaaaatt
     6541 ccggctcgcc gtggcgaagc gattaatcag ttaaaacgca tcaatgcgga tgcaccgggc
     6601 aatacgggcc tgcaaaacaa tctggcgcta ttgctgttta gtagcgatcg ccgtgacgaa
     6661 ggttttgccg tcctggaaca gatggcaaaa tcgaacgccg ggcgcgaagg ggcctctaaa
     6721 atctggtacg ggcagattaa agacatgccc gtcagtgatg ccagtgtgtc ggcgctgaaa
     6781 aaatatctct cgatctttag tgatggcgat agcgtggcgg ctgcgcaatc gcaactggca
     6841 gaacagcaaa aacagctggc cgatcctgct ttccgcgctc gtgcgcaagg tttagcggcg
     6901 gtggactctg gtatggcggg taaagccatt cccgaactac aacaggcggt gcgggcgaac
     6961 ccgaaagaca gtgaagctct gggggcgctg ggccaggcgt attctcagaa aggcgatcgc
     7021 gccaatgcag tggcgaatct ggaaaaagcc ctcgcactgg acccgcacag cagcaacaac
     7081 gacaaatgga acagtctgct gaaagtaaac cgctactggc tggcgatcca gcagggcgat
     7141 gctgcgctga aagccaataa tcctgaccgg gcagaacgcc tgttccagca ggcgcgtaat
     7201 gtcgataaca ccgacagtta tgcagtgctg gggctgggcg atgtggcgat ggcgcgaaaa
     7261 gattatcccg ccgccgaacg ttattatcag cagaccttgc gtatggacag cggcaacact
     7321 aacgccgtgc gcgggctggc aaatatttac cgccagcaat cgccagaaaa agctgaagcg
     7381 tttatcgcct cgctctctgc cagtcagcgg cgtagcattg atgatatcga acgcagcctg
     7441 caaaacgacc gtctggcaca gcaggcagag gcactggaaa accagggcaa atgggcgcag
     7501 gcggcagcac ttcagcggca acgactggcg ctggaccccg gcagcgtatg gattacttac
     7561 cgactttcgc aggatctctg gcaggccgga caacgcagcc aggccgatac gttaatgcgc
     7621 aatctggcgc agcagaagtc gaacgacccg gagcaggttt acgcttacgg gctgtacctc
     7681 tctggtcatg accaggacag agcggcgctg gcgcatatca atagcctgcc gcgtgcgcag
     7741 tggaacagca atattcagga gctggttaat cgactgcaaa gcgatcaggt gctggaaacc
     7801 gctaaccgcc tgcgagaaag cggcaaagag gcagaagcgg aagcgatgct gcgccagcaa
     7861 ccaccttcca cgcgtattga cctcacgctg gctgactggg cgcaacaacg acgtgattac
     7921 accgccgccc gcgctgcata tcagaatgtc ctgacgcggg agccagctaa cgccgacgcc
     7981 attcttggtc tgacggaagt ggatattgct gccggtgaca aagcggcggc acgtagccag
     8041 ctggcgaaac tgcccgctac cgataacgcc tcgctgaaca cacagcggcg cgtggcgctg
     8101 gcacaggcgc agcttggcga taccgcagca gcgcagcgga cgtttaataa gttgatcccg
     8161 caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg tgctgcgtga tggtgcgaag
     8221 tttgaagcgc aggcgggcga tccaacgcag gcgctggaaa cctacaaaga cgccatggtc
     8281 gcatccggtg tgactacgac gcgtccgcag gataacgaca cctttacccg actgacccgt
     8341 aacgacgaga aagatgactg gctgaaacgt ggcgtgcgca gcgatgcggc ggacctctat
     8401 cgccagcagg atcttaacgt cacccttgag cacgattact ggggttcgag cggcaccggt
     8461 ggttactccg atctgaaagc gcacactacc atgttgcagg tggatgcgcc gtattctgac
     8521 gggcggatgt tctttcgcag tgatttcgtc aatatgaacg tcggcagttt ctccactaat
     8581 gccgatggca aatgggatga caactggggc acctgtacat tacaggactg tagcggcaac
     8641 cgcagccagt cggattccgg tgccagcgtg gcggtcggct ggcgaaatga cgtctggagc
     8701 tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg atgtggtcgg cggcatcagt
     8761 tacagcgatg atatcgggcc gctgggttac accgttaacg cccaccgtcg gcccatctcc
     8821 agttctttgc tggcctttgg tgggcaaaaa gactccccga gcaataccgg gaaaaaatgg
     8881 ggtggcgtac gtgccgacgg tgtggggcta agtctgagct acgataaagg tgaagcaaac
     8941 ggcgtctggg catcgcttag tggcgaccag ttaaccggta aaaatgtcga agataactgg
     9001 cgcgtgcgct ggatgacggg ctattactat aaggtcatta accagaacaa tcgccgcgtc
     9061 acaatcggcc tgaacaacat gatctggcat tacgacaaag atctgagtgg ctactcactc
     9121 ggtcagggcg gttactacag tccgcaggaa tacctgtcgt ttgccatacc ggtgatgtgg
     9181 cgggagcgca cggaaaactg gtcgtgggag ctgggtgcgt ctggctcgtg gtcgcattca
     9241 cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga tcccgaccga ctggcaggaa
     9301 gaagctgcgc ggcaatccaa cgatggcggc agcagtc
//
