LOCUS       NZ_PJEE01000001        11905 bp    DNA     linear   CON 23-DEC-2019
DEFINITION  Bifidobacterium longum strain APC1464 contig0001, whole genome
            shotgun sequence.
ACCESSION   NZ_PJEE01000001
VERSION     NZ_PJEE01000001.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07958349
            Assembly: GCF_002833185.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bifidobacterium longum
  ORGANISM  Bifidobacterium longum
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Arboleya,S.
  TITLE     Bifidobacterium longum APC/DPC strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11905)
  AUTHORS   Arboleya,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2017) Microbiology, UCC, Western Road, Cork,
            Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2016
            Assembly Method                   :: Velvet v. 1.2.10
            Expected Final Version            :: yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2019 02:20:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,946
            Genes (coding)                    :: 1,873
            CDSs (with protein)               :: 1,873
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 48 of 73
            Pseudo Genes (internal stop)      :: 10 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            PJEE01000001.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig0001"
                     /strain="APC1464"
                     /isolation_source="fecal sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /country="Ireland"
                     /collection_date="30-Oct-2015"
     gene            1..1350
                     /locus_tag="APC1464_RS01880"
                     /old_locus_tag="APC1464_0329"
     CDS             1..1350
                     /locus_tag="APC1464_RS01880"
                     /old_locus_tag="APC1464_0329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SBP_bac_1"
                     /protein_id="WP_014485873.1"
                     /translation="MRNTKKVIAATAAVATLVGLAACGSSSDNSANKGDADKAELVFWG
                     WDSGNSMKEILADFEKANPGITVKFNNTGTAEKTSTALSNAIAAGNGAPDVVMLEDPTV
                     TQFAVTEGLVDLSEFGADKLADDFAAGPWNKLQYNNKPYALPIDSGPEMFFYNKAVFDK
                     AGVDGESIKTWDDYYEAAKKIRATGSYITNLAGTSNDYQPFTAQIWQAGAQPWKVDGEN
                     ITINMTKDEGMQRYIQFVQKLIDEDLVDTKTPNWSDDWNRELNDGTLASLTIGAWMPIN
                     LMGGAPDQAGNWRVAQLPQWEEGKEVSAEDGGSALAITAQSKNQAAAYKLVEYMTYGEG
                     AQTMADTGTFPALKKILQSDSFTDPTAESHKKTNDYFGGQNVNEVLSAAAQRSTEKFQY
                     LPYNPYAQNAYGDEVSKAYSGDITLEKALENYANKLAEQGKQQGYNVTVK"
     gene            1571..2533
                     /locus_tag="APC1464_RS01885"
                     /old_locus_tag="APC1464_0330"
     CDS             1571..2533
                     /locus_tag="APC1464_RS01885"
                     /old_locus_tag="APC1464_0330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|D6ZW63|3.A.1.1.48"
                     /protein_id="WP_077321653.1"
                     /translation="MSDQTMAAAPQPAVGILPEKADLHTAEARVNKHKADWRGWKFMWP
                     FAVVFVFVFIIPIVYAIYISFFKKQMVGGTKFVGFENYARLIQDPQFWSSVGRVTIFTV
                     FQVPIMLFLAAAMALALDSMKLHGTKFFRISTFLPYAVPAVVSTMIWGFVYGAKYGLVG
                     SLNDFLGTNIDVLNPDVLLASIANINTWEYAGYNMLIFYSSLSTIPHSLYEAASIDGAS
                     EWQIVKKIKLPELKGSLAITVIFSIIGSFQLFNEPSILQNMVPGNAITTYYTPNMYAYN
                     LSFTGNQSNYAAALAIVMAIITMAIAYVVQLKSMKEQMK"
     gene            2536..3543
                     /locus_tag="APC1464_RS01890"
                     /old_locus_tag="APC1464_0331"
     CDS             2536..3543
                     /locus_tag="APC1464_RS01890"
                     /old_locus_tag="APC1464_0331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|D6ZW62|3.A.1.1.48"
                     /protein_id="WP_014485871.1"
                     /translation="MSTATMTSTTTMTPADLKAQEKAAKKAEKERQKRIDADEKAERKR
                     AAKTGFSNPANPTRSMALTIVCAIFAVYCLFPFVYLMINATKTQADFTSTFGLGFGKTF
                     ALFDNIATVFTYQDGIFGRWFVNTILYVVVGAGGATLLAIMGGYALAKFRFPGRKLCFV
                     IIIGAISVPGIALAVPQFLLFAKMGLTNTPWSMIIPSLVSTFGLYLMWIFSDQAVPDEL
                     LEAARVDGAGEFRTFFQISLPLLAPGIVTTALFAIVATWNNYFLPLIMLKDSNWYPLTI
                     GLNQWKDQASTAGGQAIQNLVITGSLITIIPLVIAFLCLQKYWQSGLAAGAVKE"
     gene            3735..5867
                     /locus_tag="APC1464_RS01895"
                     /old_locus_tag="APC1464_0332"
     CDS             3735..5867
                     /locus_tag="APC1464_RS01895"
                     /old_locus_tag="APC1464_0332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH42"
                     /protein_id="WP_077321654.1"
                     /translation="MTTRRAFRWPSLLTPNGRGIAFGGDYNPDQWPEETLDEDIHLMTQ
                     AGVNTVALAIFSWDKIEPREGEFTFEWLDHVIDKLGAAGIAVDLASATATAPLWLYERH
                     PEVLPIDRYGHVVNAGSRQSWQPTSPVFKEYALRLCRKLAEHYKDNPYVAAWHMGNEYG
                     WNNRYDYSDNALAAFRTWCEAKYGTVDALNEAWGTAFWSQHVNSFDEVLLPRHMGGDSM
                     VNPPQQLDYERFGNDMLLDFYKAERDAIEEICPGKPFTTNFMVSTDQCTMDYAQWANEV
                     DFVSNDHYFHEGESHLDELACSDALMDSLALGKPWYVMEHSTSAVQWKPLNTRKRAGEL
                     MRDSLAHVAMGADAINFFQWRQSASGAEAFHSAMVPHAGSDTKLFRGVCELGAALKTLS
                     DAGVQDTELKRADTAILFSAESEWATRSETLPSMKLNHWHDVRDWYRGYLDAGARADVV
                     PLAYDWSGYQTIVLPTVIALSDEDTRRIADFAENGGTVIVGYATGLIDEHFHIGFGGYP
                     GAGNGLLRDMLGIRSEEFNILGEEAEDEPAEIGLSNGLTTRLWQNDVTSVAPDTCVLAT
                     YVGTAAADWELDGVPAITSRTYGNGTAIYVGCDLGRHDIARLLKEFGSCWRELSAQPTE
                     GGQTPTYPTTDPRILHTIRRSADGSTRFDFYLNRSNQPVAVNGIEGEPIIAYRCEADTT
                     GYTLNRNAILIAKASC"
     gene            5911..6966
                     /locus_tag="APC1464_RS01900"
                     /old_locus_tag="APC1464_0333"
     CDS             5911..6966
                     /locus_tag="APC1464_RS01900"
                     /old_locus_tag="APC1464_0333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="WP_077321655.1"
                     /translation="MSDEKTAKRRVTLRDVAQAAGVSLKTASNVINGSGRMTDETRAKV
                     ESVIKDLDYRVNVAARNLNRDHTGFITLAVPSLIPPYLAELANRTIDAARQRDYSVYVT
                     TYAEGSAKGARDLLKNFNSTVSDGMILSMSEVEDISPEDLKVDFPLVIVGARTTWGVAD
                     HVTPDDVAAAATAAGYLYDRGSSRLAVVGARDDYDAAALLQAVEGNAQLRLRGVIEETR
                     RRGLELDPQLIGNTGQDWTIGAGARVIQRLIDSGVPFDGVIALNDQLAIGALTALRTAG
                     YEVPAQVQVIGFDNNEEAPYLQIPLTTMDSRLDWTAPTAVNRILGRIDGSITKPELLTT
                     ESQVIARASTR"
     gene            7327..8928
                     /locus_tag="APC1464_RS01905"
                     /old_locus_tag="APC1464_0334"
     CDS             7327..8928
                     /locus_tag="APC1464_RS01905"
                     /old_locus_tag="APC1464_0334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007054844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P23936|2.A.2.2.1"
                     /protein_id="WP_100986587.1"
                     /translation="MYLMTASTESSTQSHHDEKEAIAPARLVAYALGNFGQSAFYNALS
                     TYFVVYVTNSLFSGVDKAVAGKLIGIITSLIVIIRIAEIFVDPLLGNLVDNTTTKLGRF
                     RPWQLIGGLVSAVLIVMVYTGLFGLVNVNTTLFIVLFVVVFVVLDVFYSLRDISYWGMI
                     PSISSDSHQRGIYTAAGTFTGSLGYNGLTIAVMPIVTFFSFDAGQQNQNGWTAFIIIVA
                     LLGALTALSVVFGVKEKQSTLRTKAQENGNPIEAFRALIHNDQLMWTALSYVFYAVANV
                     ATGGVMVYLFKYVIGRMDLFPVTGVIPIACGLIMSVLFPVITRWVPRRYLFLIGMSVMV
                     VAYLLLIVASTDIPLLLVALVLFYLPQTQIQMTVIVCLEDSIEYGQLKTGKRNEAVTLS
                     VRPMLDKIAGAFSNGFVGFVAVAAGMIGSATAADMTAKDITIFKTFAFMIPLVCIVLGL
                     IVFAIFVKIDEKAHARLVAKLTENLATAGDGGGNDDGAGVGDNGEHNGGHASIASEDSA
                     LAEYGPELAKRVLLGK"
     gene            9285..9608
                     /locus_tag="APC1464_RS01910"
                     /old_locus_tag="APC1464_0335"
     CDS             9285..9608
                     /locus_tag="APC1464_RS01910"
                     /old_locus_tag="APC1464_0335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007054846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007054846.1"
                     /translation="MAETSWAYTLDDGDDDSNTVPSKVTADNLKKYDISPQGQADEIRM
                     VAEAVNNIGDNDGDGDKTNGANGNKATGVLSKTGSAVTAVGLAVVVLGAAGGVSFALRR
                     KRS"
     gene            complement(9751..>10026)
                     /locus_tag="APC1464_RS01915"
                     /pseudo=""
     CDS             complement(9751..>10026)
                     /locus_tag="APC1464_RS01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695462.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /protein_id="NP_695462.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9990..10280)
                     /locus_tag="APC1464_RS01920"
     CDS             complement(9990..10280)
                     /locus_tag="APC1464_RS01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007051370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007051370.1"
                     /translation="MLLYCCDYTPLYTVMWVMCGRCAGSVRVNGMIMTGLPCGKAGHNH
                     DNITLYSCDRGLVPLFPDANLPPTTMMPSAIAEPLFPSSGHEFVPVKTSID"
     gene            10289..11158
                     /locus_tag="APC1464_RS01925"
                     /old_locus_tag="APC1464_0336"
     CDS             10289..11158
                     /locus_tag="APC1464_RS01925"
                     /old_locus_tag="APC1464_0336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="WP_011068388.1"
                     /translation="MTNGHINPANVPDIALKDGHSIPQVGLGVLRIDDEGVVPVVESAL
                     EAGYRHIDGAAGYNNEAGVGRALAASGYNKGAKRETLWVTTKLRDSQQGYDSALKAFDN
                     SLKLLQLDYVDMYMIHWPTPFDWRSTDTWKAFVKLRDEGMARTLGVCNFMPADLKRLHE
                     ETGAWPAVNQIELHPTWQQREVVAFCKEHGIAVEAYSPMARGADLNAGDGTIEKIAAAH
                     EVSPAQVILRWHIENGTIIIPKSVHADRQKENLNLFGFALTADEHAAIDALDGPTRAGH
                     DPLTFTYA"
     gene            11222..11905
                     /locus_tag="APC1464_RS01930"
                     /old_locus_tag="APC1464_0337"
     CDS             11222..11905
                     /locus_tag="APC1464_RS01930"
                     /old_locus_tag="APC1464_0337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="WP_075662544.1"
                     /translation="MLILIVLAVAIGVTIGLLLPQVSSDVAKITGGYTATGSAADTLNA
                     LAVDDNQSSSGYDRDSFGFRTTDVDGNGCDVRDDVLARDLTDITYKYAGSCVVESGTLA
                     DPYTAQTIHFVRGRATSAKVQIDHVVALENAWQSGAHDWSTAKRHEFGNDPYNLLAVDG
                     PANQEKGSASAAYWLPTNADYRCDYVARQIGVKDKYQLTVTSQEKDAMLAVLHTCPGQA
                     VPADE"
ORIGIN
        1 atgcgcaaca ccaagaaggt catcgcagca acggccgccg tggccacgct cgtcggtctc
       61 gccgcctgtg gcagcagcag cgacaacagc gccaacaagg gtgacgccga caaggccgaa
      121 ctcgtgttct ggggctggga ttccggcaac tccatgaagg aaatccttgc cgacttcgag
      181 aaggccaacc ccggcatcac cgttaagttc aacaacaccg gcaccgccga gaagacttcc
      241 accgcgctct ccaacgccat cgccgcaggc aacggcgccc ctgacgtcgt gatgctcgaa
      301 gatccgaccg tcacccagtt cgccgtcacc gagggcctcg tggacctgag cgaattcggc
      361 gccgacaaac tcgccgatga cttcgccgcc ggcccgtgga acaagctcca gtacaacaac
      421 aagccgtacg ccctgccgat tgattccggc cccgagatgt tcttctacaa caaggcagtg
      481 ttcgacaagg ccggcgtgga cggcgagtcc atcaagacct gggacgacta ctacgaggcc
      541 gccaagaaga tccgtgccac cggctcctac atcaccaatc tggccggtac ctccaacgac
      601 tatcagccgt tcacagccca gatctggcag gccggcgccc agccgtggaa ggtcgatggc
      661 gagaacatca ccatcaacat gaccaaggac gagggcatgc agcgctatat ccaattcgtc
      721 cagaagctca tcgacgaaga cctcgtggac actaagaccc cgaactggtc cgacgattgg
      781 aaccgcgagc tcaacgatgg caccctcgcc tccctgacca tcggcgcttg gatgccgatc
      841 aacctcatgg gcggcgcccc cgatcaggct ggcaactggc gtgtcgccca gctgccgcag
      901 tgggaggaag gcaaggaagt ctccgccgaa gacggtggct ccgcacttgc catcaccgcg
      961 cagtccaaga accaggccgc tgcctacaag ctcgttgagt acatgactta tggcgagggc
     1021 gctcagacca tggccgacac tggtaccttc ccggccctga agaagatcct ccagtccgac
     1081 tccttcaccg acccgaccgc cgagtcccac aagaagacca acgactactt cggtggtcag
     1141 aacgtcaacg aagtcctttc cgccgcggct cagcgatcga ccgagaagtt ccagtacctg
     1201 ccgtacaacc cgtacgccca gaacgcttac ggcgacgaag tctccaaggc ttactccggc
     1261 gacatcaccc tggagaaggc gcttgaaaac tatgccaaca agctcgccga gcaaggcaag
     1321 cagcagggct acaacgtcac cgtcaagtga cgcaatccga tttattgccg gtacatccgg
     1381 cactgaccaa caatcaaaac ctgcggctag gccactctcc ttccgcctag ccgcccgatc
     1441 tcccaaagcg gggatgctcc ccagtgtccc cgctttgcca tgccctcacc tgacgtgacc
     1501 gttccaatcg agcgaaccct ctgttagcgg gcgcagcaca gcaatccgta tcccaagaaa
     1561 gcaatcgatt atgtccgatc aaacaatggc cgcagccccg cagccagcgg tgggaattct
     1621 gcctgagaag gcggatctgc acacggccga ggctcgcgtc aacaagcaca aggccgactg
     1681 gcgtggatgg aagttcatgt ggccgttcgc cgtggtgttc gtcttcgtgt tcatcatccc
     1741 gattgtgtac gccatttaca tctccttctt caagaagcag atggtcggcg gcaccaagtt
     1801 cgtcggcttc gagaactacg cccgactgat tcaggatccg cagttctgga gctcggtggg
     1861 ccgcgtcact atcttcaccg tattccaggt gccgatcatg ctgtttctgg ccgcagccat
     1921 ggccctcgct ttggattcga tgaagctgca cggtaccaag ttcttccgta tctccacgtt
     1981 cttgccttac gcggtgcccg cagtggtctc caccatgatt tggggcttcg tgtatggcgc
     2041 caaatacggt ctggtcggct ccctcaacga cttcctcggc accaacatcg atgtgctcaa
     2101 ccccgacgtg ctgcttgcat ccatcgcgaa catcaacacg tgggaatacg ccggctacaa
     2161 catgctgatc ttctacagct ccctgagcac cattccgcac agcctgtatg aggccgccag
     2221 catcgacggc gcttcggagt ggcagattgt taagaagatc aagctgccgg agctgaaggg
     2281 ctcgctggcc atcacggtga tcttctcgat catcggcagc ttccagctgt tcaacgagcc
     2341 gagcattttg cagaacatgg ttccgggcaa cgcgatcacc acgtactaca cgccgaacat
     2401 gtacgcctac aacctgagct tcaccggcaa ccagagcaac tatgccgcag ctctggccat
     2461 cgtgatggcc atcatcacca tggccattgc gtatgtcgtc cagcttaaga gtatgaagga
     2521 gcagatgaag taattatgag cactgcaacc atgacttcaa caacaaccat gaccccggcc
     2581 gatctcaagg ctcaggaaaa ggccgccaag aaggccgaga aggagcgtca gaagcgcatc
     2641 gacgccgacg agaaggccga gcgcaagcgc gccgccaaga ccggtttctc caacccggcg
     2701 aacccgaccc gctcgatggc cttgaccatc gtgtgcgcga ttttcgcggt ctactgcctc
     2761 ttcccgtttg tctacttgat gatcaacgcc acgaagacgc aggccgattt cacctcgacg
     2821 ttcggtctgg ggttcggcaa gacgttcgct ttgttcgaca acatcgcgac cgtgttcacc
     2881 taccaggacg gtatcttcgg ccgctggttc gtcaacacga ttctgtatgt ggtggtcggc
     2941 gccggcggtg ccaccttgct ggccatcatg ggcggctatg cgctggccaa gttccgcttc
     3001 cccggccgca agctgtgctt cgtgatcatc atcggcgcca tttcggtgcc gggtatcgcg
     3061 ctggccgtgc cgcagttcct gctgttcgcc aagatgggcc tcacgaacac cccgtggtcg
     3121 atgattatcc cgagtttggt atccacgttc ggtctgtatc tgatgtggat cttctccgat
     3181 caggccgtgc cggatgagct gcttgaggcc gcgcgcgtgg atggtgccgg cgagttccgc
     3241 accttcttcc agatttccct gccgctgctg gcacccggca tcgtgaccac tgcattgttc
     3301 gccattgtgg cgacgtggaa caactacttc ctgccgttga tcatgttgaa ggattccaac
     3361 tggtatccgt tgacgatcgg tctgaaccag tggaaggacc aggcttcaac cgcaggcggt
     3421 caagctatcc aaaacctcgt gattaccggt tcgctgatca cgatcatccc tctggtgatt
     3481 gcgttcctgt gcctgcagaa gtactggcag tccggcctcg ctgcaggcgc cgtcaaggag
     3541 tagttgctac tctttgacgg ttctcctcag tccgcttcgc agatagctcc cctcagagag
     3601 gggagctgaa gaaatggggt cttgtctccc ttttctgagg agagctgtcg aacgcagtga
     3661 gactgagggg caaagcccaa aactggcaaa cacagcaccg gccataagcc gatacaataa
     3721 aggacttgat tgatatgact actcgcagag cattccgttg gccgtctttg ttgacgccga
     3781 atggtcgtgg catcgcgttc ggcggtgact acaatccgga tcagtggccg gaggagacgc
     3841 tggacgagga catccacctg atgacccagg ctggcgtcaa cactgtggcc ctcgccatct
     3901 tcagctggga caagatcgaa ccgcgcgagg gcgagttcac gttcgagtgg cttgaccacg
     3961 tgatcgacaa gctcggcgcg gcgggcatcg ccgtggacct ggcctcggcc actgccacgg
     4021 ccccgttgtg gctgtacgag cgtcatcctg aggtgctgcc aatcgatcgg tacggccatg
     4081 tggtcaacgc gggctcacgt cagtcgtggc agcccacaag cccggtgttc aaggaatacg
     4141 cgttgcgcct gtgccgcaaa cttgccgaac actacaagga caacccgtat gtggccgcct
     4201 ggcacatggg caacgaatac ggctggaaca accgctacga ctactccgac aacgccttgg
     4261 ccgcgttccg cacgtggtgc gaggccaaat acgggaccgt cgacgcgttg aacgaagcgt
     4321 ggggcacggc cttctggtcc cagcacgtga acagcttcga cgaggtgttg ctgcctcgcc
     4381 acatgggcgg cgacagcatg gtcaacccgc cccagcagtt ggattacgag cggttcggca
     4441 acgacatgct gctcgacttc tacaaggccg aacgcgacgc aatcgaagaa atctgccccg
     4501 gcaagccgtt caccacgaac ttcatggtct ccaccgacca atgcaccatg gactacgcgc
     4561 aatgggcaaa cgaagtggac ttcgtgtcca acgatcacta cttccatgaa ggcgaatccc
     4621 atctggacga actcgcctgc tcggacgcgc tcatggactc gctcgcgctc ggcaagccgt
     4681 ggtacgtgat ggagcactcc acttcagccg tgcagtggaa gccccttaac acgcgcaagc
     4741 gcgccggcga gctcatgcgc gactccttgg cccacgtggc catgggtgcc gacgccatca
     4801 acttcttcca atggaggcaa tccgcatcgg gtgccgaggc gttccactcc gcgatggtgc
     4861 cccacgcggg ctccgatacg aaactgtttc gaggtgtgtg cgaactcggc gccgcgctga
     4921 agacgctgtc cgacgcgggt gtgcaagaca cggaactgaa gcgcgcggac acggcgattc
     4981 tgttcagtgc ggaatccgaa tgggccactc gctccgagac cctgcccagc atgaaactca
     5041 atcactggca tgacgtacgc gactggtatc gcggatatct cgatgccgga gcgcgcgcgg
     5101 acgtggtccc gctcgcctac gattggagcg gctaccagac tatcgtgctg cccaccgtga
     5161 tcgccctgtc cgacgaggat acccgtcgca tcgcggactt cgccgaaaac ggcggtacgg
     5221 tcatcgtcgg ctatgccacc ggcctgatcg acgaacactt ccacatcggt ttcggcggat
     5281 accccggcgc cggcaacgga cttctgcgcg acatgctcgg catccgctcc gaagaattca
     5341 acatcctcgg agaagaagcc gaagacgaac cggccgaaat cggtttgtcg aatggactca
     5401 cgacccgtct gtggcagaat gacgtgacct cggtcgctcc agacacttgc gtgcttgcca
     5461 cgtacgtggg tacagccgct gctgattggg agctcgacgg cgtccccgcc atcaccagcc
     5521 gcacctatgg caacggtacc gccatctacg tggggtgcga ccttggccgc cacgacattg
     5581 cccgcctgct taaggagttc ggctcctgct ggcgggagct gtcggcgcag ccgactgagg
     5641 gtggtcaaac tccaacctat cccaccacag atccccgcat cctgcacacc atccgccgat
     5701 ccgcagatgg ctccactcgc ttcgatttct acctcaaccg ctcaaaccag cccgttgccg
     5761 tcaacggcat tgagggagaa ccaatcatcg cctaccgttg cgaggccgac accaccggct
     5821 atacgctgaa tcgcaatgcc attcttattg ctaaagcgtc ctgctagacc gcgctagaat
     5881 ggcaatgttg tccagataga ggagtgcggc atgagcgacg agaaaacagc caagcgtcga
     5941 gtgacgttac gcgatgtcgc tcaggccgct ggtgtatcgc tcaaaactgc gtcgaacgtg
     6001 atcaatggct ctgggcgtat gaccgatgag acccgtgcca aggtcgagtc ggtcattaag
     6061 gacctcgact accgagtcaa cgtggccgcc cgcaacctca accgcgatca caccggtttc
     6121 atcacccttg ccgtgccttc gctgattccg ccatatctgg ccgagttggc caatcgcacc
     6181 attgacgccg cccgtcagcg cgactactcc gtatacgtca ccacgtatgc agagggctcg
     6241 gccaaaggag cgcgcgacct gctcaagaac ttcaactcca ccgtgtcaga cggcatgatt
     6301 ctctccatga gcgaggtgga ggatatttcg cccgaagacc tgaaggtcga tttcccgctg
     6361 gttattgtcg gtgcccgaac cacatggggt gttgccgacc acgtgactcc ggacgatgtt
     6421 gccgccgcag caacagcggc aggctaccta tacgatcgag gttcttctcg cctcgctgta
     6481 gtgggcgcac gagacgacta tgacgcggcc gctttgttgc aggctgtgga aggcaacgca
     6541 caactgcgtt tgcgtggtgt cattgaagaa acgcgccgcc gtgggcttga gctggatccg
     6601 cagctgattg gcaacaccgg tcaggattgg accatcggag ccggtgcccg tgtgatccag
     6661 cggctcattg attccggcgt gccattcgac ggtgtgatcg ccctgaacga tcagttggcc
     6721 atcggcgcat tgaccgctct gcgcaccgct ggttatgagg tgcccgcgca ggtgcaggtc
     6781 atcggcttcg acaacaacga ggaggccccc tacctgcaga ttccgctgac caccatggat
     6841 tcccgccttg actggacggc tcccacggca gtgaatcgca tcctcggacg catcgacggc
     6901 agcatcacca aacccgaact gctcaccacc gaatcccaag tcatcgcccg cgcctccaca
     6961 cgctgacgct aatctggctc ccctccctgg ggggagctgt caccgaaggt gactgagggg
     7021 agccttgcgg aacattgtca tctcccgtcg taactcgtaa ttgtcactcg tttgagtggc
     7081 cccatgtgtc atcggaggta gccgttcact ccgctccgag ttcgtgcatc tctccccccc
     7141 ccccacgata agagacgccg ttcaatcgtt tagtgacttt ttgttaattt ctgtgcttaa
     7201 gtagcaactt tatgtgtgtt ttctctacga tctggtgtat attatttttt ggtttaccgg
     7261 tgtatcgcac gcgcgatgtg tgaattgccg caacatcgct gatatgcgta catggaatct
     7321 ggaggaatgt atctgatgac ggcatcaact gaatcgtcta cgcaatccca tcatgatgag
     7381 aaagaggcca ttgctcccgc gcgacttgtg gcctacgcgc tcggcaactt cggccagtcg
     7441 gcgttctaca acgcgctaag cacctacttc gtggtctatg tgaccaactc gctgttctcc
     7501 ggagtggaca aggccgtggc gggcaagctc atcggcatca tcacctcgct gatcgtgatc
     7561 attcgtatcg cggaaatctt cgtggacccg ctgctgggca acctggtgga taacaccacc
     7621 accaaactcg ggcgattccg cccttggcag ctcatcggcg gtctggtgtc cgccgtgctg
     7681 atcgtcatgg tctacactgg cctgttcggc ttggtcaacg tcaacaccac gctgttcatc
     7741 gtcctgttcg tggtggtgtt cgtggtgctc gatgtgttct actccctgcg cgatatctcc
     7801 tactggggaa tgatcccctc catctcttcc gactcccatc agcgcggcat ctacaccgca
     7861 gccggcacct tcaccggatc gctcgggtac aacggtctga ctatcgccgt gatgcccatc
     7921 gtcaccttct tcagcttcga tgccggccag cagaaccaga atggttggac cgcgttcatc
     7981 atcatcgtgg ccctgctcgg cgctctcacg gctctgagtg tagtgttcgg tgtcaaggaa
     8041 aaacagagca cgctgcgcac caaggcccag gagaacggta acccgattga agcgttccgc
     8101 gccctgattc acaacgacca gctcatgtgg accgctttgt cttatgtgtt ctacgccgtc
     8161 gccaacgtgg ccaccggtgg cgtgatggtc tacctgttca aatacgtgat tggtcgcatg
     8221 gaccttttcc cggtcaccgg cgtcatccct attgcgtgcg gtctgattat gtcggtactg
     8281 ttcccggtca tcactaggtg ggtgccccgc cgttatctgt tcctcatcgg catgtccgtg
     8341 atggtcgtgg cctacctgct gctgattgtc gcctccacgg atataccgct gctgctggtg
     8401 gcactagtgc tgttctacct gccgcagacc cagattcaga tgaccgtgat cgtgtgcctt
     8461 gaggactcca ttgaatacgg ccagctgaaa acaggcaaac gcaacgaagc cgtgacgctg
     8521 tccgtgcgtc cgatgctcga caagatcgcc ggcgcattct ccaacggctt cgtcggcttc
     8581 gtggccgtgg ccgccggcat gatcggttcc gccaccgccg ccgacatgac ggccaaggac
     8641 atcaccatct tcaagacctt cgccttcatg attccgctgg tgtgcattgt gcttggcctg
     8701 attgtgttcg cgattttcgt gaagatcgac gaaaaggccc atgcgcgtct ggtcgccaag
     8761 ctcactgaga atcttgcgac ggctggcgat ggtggtggta acgacgatgg tgctggtgtt
     8821 ggtgacaatg gtgagcataa tggtggccac gccagcatcg cgtcggaaga ctccgcattg
     8881 gcggaatacg gtcccgaact cgccaagcgg gtgttgctcg gcaaatagtg ttgctgtgcg
     8941 aatgagctgt ctcatggggt ggtgattggc ccatggggcg gtgaatccct catggggcgg
     9001 tagctgaaaa tggctctatt ctgcggaagg taaaatttta ccgaccgccc catgggcaag
     9061 ttaccgcccc atgggatttt cgccgcccca tggatgtacg ggtaaaggcg gacgaattgc
     9121 ctcgtgccgc cctcgtggat atgcaagtta aagagcatcg gcagcatgac tgaatgcaca
     9181 tagagcgtcc cccgcatggt tgtgccacgt tccatggcgg ggtcgccgtg gtgactgtcg
     9241 ccagtacacg ccatgccacg cgagcacgtt gttgccggtg ttccatggcg gaaacgtcgt
     9301 gggcatacac cttggatgat ggcgacgacg attccaacac agtacccagt aaagtaaccg
     9361 ccgataacct caagaaatac gacatcagcc cgcaaggcca ggccgatgag attcgcatgg
     9421 tcgccgaggc cgtgaacaat atcggcgaca acgatggcga tggcgataag accaacggtg
     9481 cgaatggcaa caaggctact ggcgtgctga gcaagaccgg tagcgcggtg actgccgtgg
     9541 gtcttgcggt cgtcgttctc ggtgcagccg gcggggtctc gtttgctttg cgtcgtaagc
     9601 gctcctgata aggactgact cggcattatg atgcggttgc cagttgctgg ctgtcggctg
     9661 tcgtctgcca gatgcggctg tctcggcgta gcgatgcgat tctgtcccgg ttcgcgcatc
     9721 aatcgattga tgttaatacc gggacagaaa tcattgtccc ggtttgaaaa aacgggcgtt
     9781 tttcgcgtac cgggacaatt ctcgttgccc cgatacgagc gtcaatcgtt ttatgtgcga
     9841 accgggacaa catgtcgatc tcgggattga cggtattccg ccgattctgc ttgtcggggt
     9901 atgtggactt tgaaaattgg tttgtcccgg ttctcaaaaa tgggccgaaa ttgcgtaccg
     9961 ggacaacgat ttctgtcccg gtagaaacgt caatcgattg acgttttcac cgggacaaat
    10021 tcgtgcccag aggaggggaa gaggggctcc gcaatggcgc tgggcatcat tgtggttgga
    10081 ggcaaattcg cgtccgggaa caatgggacg aggccccggt cgcaggagta gagcgtgatg
    10141 ttgtcatgat tatgaccggc tttcccacat ggaaggccag tcataatcat gccgtttacc
    10201 cgcacgctcc ctgcacaccg gccgcacatc acccacataa ccgtgtacag tggcgtgtag
    10261 tcgcagcaat aaaggagcat cgcacatcat gaccaacgga cacatcaacc ccgccaacgt
    10321 gccggacatc gccttgaaag acggtcattc cattccgcaa gtcggtctcg gcgtgctacg
    10381 tatcgacgat gagggtgtgg tccccgtcgt cgaatccgcg ttggaagccg gctaccgcca
    10441 tatcgatggt gcggccggct acaacaacga ggccggcgtg ggccgcgcgc tcgccgcttc
    10501 cggatacaac aagggagcca agcgcgagac cctgtgggtc accaccaagc tgcgcgactc
    10561 ccagcagggc tacgattccg cgctcaaagc tttcgacaac tcgctgaaac tcctccagct
    10621 cgattacgtg gacatgtaca tgatccattg gcccacgccg ttcgactggc gcagcaccga
    10681 cacctggaag gcgttcgtca agctgcgcga cgaaggcatg gcacgcacac tcggcgtatg
    10741 caacttcatg cccgccgatc tgaagcgtct gcacgaggag accggcgcat ggccggccgt
    10801 caatcagatt gagctgcacc ccacgtggca gcagcgcgaa gtggtggcgt tctgcaagga
    10861 gcacggcatc gccgtggagg cctactcgcc gatggcgcgt ggcgccgacc tcaacgccgg
    10921 cgacggcacc atcgagaaga tcgccgccgc ccatgaggtc agcccggccc aggtgattct
    10981 gcgttggcac atcgaaaacg gcacgatcat catccccaag tccgtgcatg ctgaccgcca
    11041 gaaggagaat ctcaacctgt tcggcttcgc cctgaccgcc gatgaacacg ctgccatcga
    11101 cgccctcgac ggccccaccc gcgccggcca cgaccccctc accttcacct atgcgtgatt
    11161 gatggcacgt cgtaacgccc acttccgccg tcgtttcaac gcctctggac cgctggagcg
    11221 cgtgctgatt ctcatcgttc tcgccgtagc aatcggcgtg accatcggcc tgttactgcc
    11281 gcaggtcagc tccgacgtgg ccaaaatcac cggtggctac actgccaccg gttcagccgc
    11341 cgacactttg aacgcgctcg ccgtagacga caaccaaagc tcctccggct acgaccgcga
    11401 ttccttcggt ttccgcacca ccgacgtcga cggcaatggc tgcgacgtac gcgacgacgt
    11461 gctcgcccgc gatctcacgg acatcaccta caaatacgcc ggttcctgtg tggtcgagtc
    11521 cggcacactg gccgatccgt acaccgccca gaccatccat ttcgtacgtg gccgcgccac
    11581 cagcgccaaa gtgcagatcg accatgtggt ggcccttgaa aacgcttggc aatccggtgc
    11641 gcacgactgg tccactgcca aacgccatga attcggcaat gacccctata acctgctggc
    11701 cgtcgacggt ccggccaatc aggagaaggg ttcggcttcc gccgcctact ggctacccac
    11761 caacgccgac taccgttgcg actacgtggc ccgccagatt ggcgtcaaag acaagtacca
    11821 gctcaccgtc acctcccaag aaaaggacgc catgctcgcc gtcctccata cctgcccggg
    11881 ccaggccgtg cccgcagacg aatag
//
