LOCUS       NZ_PJEG01000006        12100 bp    DNA     linear   CON 23-DEC-2019
DEFINITION  Bifidobacterium longum strain APC1461 contig0006, whole genome
            shotgun sequence.
ACCESSION   NZ_PJEG01000006
VERSION     NZ_PJEG01000006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07958347
            Assembly: GCF_002833315.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bifidobacterium longum
  ORGANISM  Bifidobacterium longum
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Arboleya,S.
  TITLE     Bifidobacterium longum APC/DPC strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12100)
  AUTHORS   Arboleya,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2017) Microbiology, UCC, Western Road, Cork,
            Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2016
            Assembly Method                   :: Velvet v. 1.2.10
            Expected Final Version            :: yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2019 02:26:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 73 of 156
            Pseudo Genes (incomplete)         :: 87 of 156
            Pseudo Genes (internal stop)      :: 16 of 156
            Pseudo Genes (multiple problems)  :: 20 of 156
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            PJEG01000006.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="Bifidobacterium longum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig0006"
                     /strain="APC1461"
                     /isolation_source="fecal sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:216816"
                     /country="Ireland"
                     /collection_date="30-Oct-2015"
     gene            1..1350
                     /locus_tag="APC1461_RS02130"
                     /old_locus_tag="APC1461_0390"
     CDS             1..1350
                     /locus_tag="APC1461_RS02130"
                     /old_locus_tag="APC1461_0390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SBP_bac_1"
                     /protein_id="WP_007055690.1"
                     /translation="MRNTKKVIAATAAVATLVGLAACGSSNDNGANKGDADKAELVFWG
                     WDSGNSMKEILADFEKANPGITVKFNNTGTAEKTSTALSNAIAAGNGAPDVVMLEDPTV
                     TQFAVTDGLVDLSEFGADKLADDFAAGPWNKLQYNNKPYALPIDSGPEMFFYNKAVFDK
                     AGVDGESIKTWNDYYEAAKKIRATGSYITNLAGTSNDYQPFTAQIWQAGAQPWKVDGEN
                     ITINMTKDEGMQRYIQFVQKLIDEDLVDAKTPNWSDDWNRELNDGTLASLTIGAWMPIN
                     LMTGAPDQAGNWRVAQLPQWEEGKEVSAEDGGSALAVTSQSKNQAAAYKLVEYMTHGEG
                     AQTMADTGTFPSLKKILRSDSFTDPTTESNKKTNDYFGGQNVNEVLSAAAQRPTEKFQY
                     LPYNPYAQTAYGDEVSKAYSGDITLEKALENYANKLAEQGKQQGYNVTVK"
     gene            1569..2531
                     /locus_tag="APC1461_RS02135"
                     /old_locus_tag="APC1461_0391"
     CDS             1569..2531
                     /locus_tag="APC1461_RS02135"
                     /old_locus_tag="APC1461_0391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|D6ZW63|3.A.1.1.48"
                     /protein_id="WP_007055699.1"
                     /translation="MSDQTMAAAPQPTVGILPEEADLHTAEARVNKHKADWRGWKFMWP
                     FAVVFVFVFIIPIVYAIYISFFKKQMVGGTKFVGFENYARLIQDPQFWSSVGRVTIFTV
                     FQVPIMLFLAAAMALALDSMKLHGTKFFRISTFLPYAVPAVVSTMIWGFVYGAKYGLVG
                     SLNDFLGTNIDVLNPDVLLASIANINTWEYAGYNMLIFYSSLSTIPHSLYEAASIDGAS
                     EWQIVKKIKLPELKGSLAITVIFSIIGSFQLFNEPSILQNMVPGNAITTYYTPNMYAYN
                     LSFTGNQSNYAAALAIVMAIITMAIAYVVQLKSMKEQMK"
     gene            2534..3541
                     /locus_tag="APC1461_RS02140"
                     /old_locus_tag="APC1461_0392"
     CDS             2534..3541
                     /locus_tag="APC1461_RS02140"
                     /old_locus_tag="APC1461_0392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|D6ZW62|3.A.1.1.48"
                     /protein_id="WP_007055725.1"
                     /translation="MSTATMTSTTTMTLADLKAQEKAAKKAEKERQKRIDADEKAERKR
                     AAKTGFSNPANPTRSMALTIVCAIFAVYCLFPFVYLMINATKTQSDFTSTFGLGFGKTF
                     ALFDNIATVFTYQDGIFGRWFVNTILYVVVGAGGATLLAIMGGYALAKFRFPGRKLCFV
                     IIIGAISVPGIALAVPQFLLFAKMGLTNTPWSMIIPSLVSTFGLYLMWIFSDQAVPDEL
                     LEAARVDGAGEFRTFFQISLPLLAPGIVTTALFAIVATWNNYFLPLIMLKDSNWYPLTI
                     GLNQWKDQASTAGGQAIQNLVITGSLITIIPLVIAFLCLQKYWQSGLAAGAVKE"
     gene            3728..5860
                     /locus_tag="APC1461_RS02145"
                     /old_locus_tag="APC1461_0393"
     CDS             3728..5860
                     /locus_tag="APC1461_RS02145"
                     /old_locus_tag="APC1461_0393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH42"
                     /protein_id="WP_008782890.1"
                     /translation="MTTHRAFRWPSLLTESGRGIAFGGDYNPDQWPEETLDEDIRLMGE
                     AGVNVVSLAIFSWDKIEPVEGAFTFEWLDHVIDRLGRAGIAVDLASATAAAPLWLYESH
                     PEVLPVDRYGHTVNAGSRQSWQPTSPVFKEYALRLCRKLAEHYKDNPYVTAWHMGNEYG
                     WNNRYDYSDNALAAFRTWCEAKYGTIDALNEAWGTAFWSQHVNSFDEVLLPRHMGGDAM
                     VNPSQQLDYERFGNDMLLDFYKAERDAIEQICPDKPFTTNFMVSTDQCVMNYAKWADEV
                     DFVSNDHYFHEGESHLDELACSDALMDSLALGKPWYVMEHSTSAVQWKPLNTRKRAGEL
                     MRDSLAHVAMGADAICFFQWRQSKSGAEAFHSAMLPHAGADSKVFRGVCELGKALKTLS
                     DAGLQGTELERAGTAILFSAESEWATRSETLPSMKLNHWHDVRDWYRGFLDAGLRADVV
                     PLAYDWTGYKTIVLPTVLSLSDEDVLRIADFAKAGGTVIVGYAAGLIDEHFHIGLGGYP
                     GAGNGLLRDMLGIRSEEFNILGEEAEGEPSEISLSNGLTTRLWQNDVTSVAADTTVLAS
                     YAGESAADWELERTPAITSRPYGNGTAIYVGCDLNRHDIAQLLKALGSRWQELSAQPTE
                     SGQTPTYPTTDPRILHTIRRSADGSTRFDFYLNRSNQPVAINGVEGDPIIAHRCETDAV
                     GYTLNRNAILIAKTSC"
     gene            5904..6959
                     /locus_tag="APC1461_RS02150"
                     /old_locus_tag="APC1461_0394"
     CDS             5904..6959
                     /locus_tag="APC1461_RS02150"
                     /old_locus_tag="APC1461_0394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="WP_008782891.1"
                     /translation="MSDEKTAKRRVTLRDVAQAAGVSLKTASNVINGSGRMTDETRAKV
                     ESVIKDLDYRVNVAARNLNRDHTGFITLAVPSLIPPYLAELANRTIEAARQRDYSVYVT
                     TYAEGSAKGARDLLKKFNSTVSDGMILSMSEVENISPDDLKVDFPLVIVGARTTWGIVD
                     HVTPDDVSAAAMAAGYLYDRGSTRLAVIGARDVYDEDALLNAVEGNAQLRLRGIIEETR
                     RRGLSLDPQLIGNTDQDWTIGAGARVTQRLIDSGVPFDGVIALNDQLAIGALTALRTAG
                     YEVPVQVQVIGFDNNEEAPYLQIPLTTMDSRLDWTAPTAVNRILGRIDGSITKSELLTT
                     ESQVIARASTR"
     gene            7155..9848
                     /locus_tag="APC1461_RS02155"
                     /old_locus_tag="APC1461_0395"
     CDS             7155..9848
                     /locus_tag="APC1461_RS02155"
                     /old_locus_tag="APC1461_0395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH53|CBM61"
                     /protein_id="WP_011068390.1"
                     /translation="MRRNTALTRIMASGVAAIMLCAGGTFTVNAAEEEPVKADVSVKAI
                     QGLSDDFIGGMDVSSMLSLEESGVTFKNANGEVEDLFTLLKESGVNYVRLRVWNDPFTA
                     DGQGYGGGNVNADRALTMAKRATAAGLKVLVDFHYSDFWADPSKQQVPKAWKSFEGDAD
                     KTADTVYDYTKQTLTTFKQAGVDVGMVQVGNETTAKIAGISGWDGMSKVFSAGSKAIRE
                     VLPEAKVVIHFTNPEKAGTYATYAKQLSNHNVDYDVFASSYYPFWHGTTENLTSVLKNV
                     ASTYKKDVMVAETSWAYTLDDGDDDSNTVPSKVTADNLKKYDISPQGQADEIRAVAEAV
                     NNIGDNDGDGENDGLGVFYWEPAWVPVGTGGKDNAELVDTWNKYGGGWATEAAGEYDPN
                     DAGLYWGGSGVDNQALFDFDGKALASLPTFKYIHTGAVTDHVFTKIDPVEITATDSDSI
                     DAIKAQLPSEVAAHYQDGVDETETVTWQSAALDWIRGAGTYTITGTTNAGHDVTVTVTV
                     TATPAKDYVTDGSFENAENDKNWTIAGTGASITEDSGNAADGKRALKFWASDAYSFSAT
                     QTITGLEPGEYVLTAMSQGAAADNAAITDGVALSATTGGKTTSDALELNGWVKFDTATV
                     PVTVGADGTATITITGNLPADAWGNVDKVSLVKKTETPVKPSTENLDKAVAEAGKINRD
                     EYTNESLAKLDQALAAADVLLAGSTYTEQDVNDVIKLVADAIAGLAQKEVSSLTVTPSK
                     TTYQVGDAIDADHDLKVVGNYSAGMGNVTLSADQFTLDYDFSAPADAAKVTVTLKSNPN
                     VTETYTVAVTARAEGGSGNGSDGAGNGGATINPDTGEGDKTNGANGDKITGVLSNTGSA
                     VTAVGLAVVVLGVAGGVSLALRRKRS"
     gene            complement(10026..10415)
                     /locus_tag="APC1461_RS02160"
     CDS             complement(10026..10415)
                     /locus_tag="APC1461_RS02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003831698.1"
                     /translation="MLQPNGMIAPGLPCGRAGRNHDNITLYSCDRPSPYCSRARICPGT
                     GVNRLTFAPGQKSLSRYAISGHFREPGQDNPQSPHTPTNRIGGIQPIPKSTYCPGSHVE
                     RLTCKPGQRELSRYAKNTRFRKPGQ"
     gene            10484..11353
                     /locus_tag="APC1461_RS02165"
                     /old_locus_tag="APC1461_0396"
     CDS             10484..11353
                     /locus_tag="APC1461_RS02165"
                     /old_locus_tag="APC1461_0396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="WP_013140277.1"
                     /translation="MTNGHINPANVPDIALKDGHSIPQVGLGVLRIDDEGVAPVVESAL
                     EAGYRHIDGAAGYNNEAGVGRALAASGYNTGAKRETLWVTTKLRDSEQGYDSALKAFDN
                     SLKLLQLDYVDMYMIHWPTPFDWRSTDTWKAFVKLRDEGMARTLGVCNFMPADLKRLHG
                     ETGAWPAVNQIELHPTWQQREVVAFCKKHGIAVEAYSPMARGADLNAGDGTIEKIAAAH
                     EVSPAQVILRWHIENGTIIIPKSVHADRQRENLDLFGFALTADEHAAIDALDGPTRAGH
                     DPLTFTYA"
     gene            11417..12100
                     /locus_tag="APC1461_RS02170"
                     /old_locus_tag="APC1461_0397"
     CDS             11417..12100
                     /locus_tag="APC1461_RS02170"
                     /old_locus_tag="APC1461_0397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_695460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="WP_075662544.1"
                     /translation="MLILIVLAVAIGVTIGLLLPQVSSDVAKITGGYTATGSAADTLNA
                     LAVDDNQSSSGYDRDSFGFRTTDVDGNGCDVRDDVLARDLTDITYKYAGSCVVESGTLA
                     DPYTAQTIHFVRGRATSAKVQIDHVVALENAWQSGAHDWSTAKRHEFGNDPYNLLAVDG
                     PANQEKGSASAAYWLPTNADYRCDYVARQIGVKDKYQLTVTSQEKDAMLAVLHTCPGQA
                     VPADE"
ORIGIN
        1 atgcgcaaca ccaagaaggt catcgcggca acggcagccg tggccacgct cgtcggtctc
       61 gccgcctgcg gcagcagcaa cgacaacggc gccaacaagg gtgacgctga caaggccgaa
      121 ctcgtgttct ggggctggga ttccggcaac tcgatgaagg aaatcctcgc tgacttcgag
      181 aaggccaacc ccggcatcac cgtcaagttc aacaacaccg gcaccgccga gaagacctcc
      241 accgcgctct ccaacgccat cgccgcaggc aacggcgccc ctgacgtcgt gatgctcgaa
      301 gatccgaccg tcacccagtt cgccgtcacc gatggcctcg tggacctgag cgaattcggc
      361 gccgacaaac tcgccgacga cttcgccgcc ggcccgtgga acaagctcca gtacaacaac
      421 aagccgtacg ctctgccgat tgattccggc cccgaaatgt tcttctacaa caaggcagtg
      481 ttcgacaagg ccggcgtgga cggcgagtcc atcaagacct ggaacgacta ctacgaggcc
      541 gccaagaaga tccgtgccac cggctcctac atcaccaatc tggccggtac ctccaacgac
      601 tatcagccgt tcaccgccca gatctggcag gccggtgccc agccgtggaa ggtcgatggc
      661 gagaacatca ccatcaacat gaccaaggac gagggcatgc agcgctatat ccaattcgtc
      721 cagaagctca tcgacgaaga cctcgtggac gccaagaccc cgaactggtc cgacgattgg
      781 aaccgcgagc tcaacgatgg caccctcgcc tccctgacca tcggcgcttg gatgccgatc
      841 aacctcatga ccggcgcccc cgatcaggcc ggcaactggc gcgtcgccca gctgccgcag
      901 tgggaggaag gcaaggaagt ctccgccgaa gacggtggct ctgcacttgc cgtcacctcg
      961 cagtccaaga accaggccgc tgcctacaag ctcgttgagt acatgactca tggcgagggc
     1021 gctcagacca tggccgacac cggtaccttc ccgtccctga agaagatcct ccggtccgac
     1081 tccttcaccg acccgaccac cgagtccaac aagaagacca acgactactt cggcggtcag
     1141 aacgtcaacg aagtcctttc cgccgcggct cagcgcccga ccgagaagtt ccagtacctg
     1201 ccgtacaacc cgtacgccca gaccgcttac ggcgacgaag tctccaaggc ctactccggc
     1261 gacatcaccc tggagaaggc gcttgaaaac tatgccaaca agctcgccga gcaaggcaag
     1321 cagcagggct acaacgtcac cgtcaagtga cgcaaccgat ctattgccgg tacatccggc
     1381 actgaccaac aatcaaaacc tgcggctagg ccactctcct tccgcctagc cgcccgatct
     1441 cccaaagcgg ggatgctccc cagtgtcccc gctttcccgt gccctcgcct gacgtgaccg
     1501 ctccaatcga gcgaccccct ggtagcgggc gcagcacagc aatccgtatt ccaagaaagc
     1561 aatcgattat gtccgatcaa acaatggccg cagccccgca gccaacggtg ggaatcctgc
     1621 ctgaggaggc ggatctgcac acggccgagg cccgcgtcaa caagcacaag gccgattggc
     1681 gcggctggaa gttcatgtgg ccgttcgccg tggtgttcgt cttcgtgttc atcatcccaa
     1741 ttgtgtacgc catttacatc tccttcttca agaagcagat ggtcggcggc accaagttcg
     1801 tcggcttcga gaactacgcc cgactgattc aggatccgca gttctggagc tcggtgggcc
     1861 gcgtcactat cttcactgta ttccaggtgc cgatcatgct gtttctggcc gcagccatgg
     1921 ccctcgcttt ggattcgatg aagctgcatg gcaccaagtt cttccgcatc tccacattcc
     1981 tgccctatgc ggtgcccgca gtggtctcca ccatgatttg gggtttcgtg tatggcgcca
     2041 aatacggtct ggtcggctcc ctcaacgact tcctcggcac caacatcgat gtgctcaacc
     2101 ccgacgtgct gcttgcatcc atcgcgaaca tcaacacgtg ggaatatgcc ggctacaaca
     2161 tgctgatttt ttacagctcc ctaagcacca ttccgcacag cttgtatgag gccgcctcca
     2221 tcgacggcgc ttcggagtgg cagattgtta agaagatcaa gctgccggag ctgaagggct
     2281 cgctggccat cacggtgatc ttctcgatca tcggcagctt ccagctgttc aacgagccga
     2341 gcattttgca gaacatggtt ccgggcaacg cgatcaccac gtactacacg ccgaacatgt
     2401 acgcctacaa cctgagcttc accggcaatc agagcaacta tgccgcagct ctggccatcg
     2461 tgatggccat catcaccatg gccattgcgt atgtcgtcca gcttaagagt atgaaagagc
     2521 agatgaagta attatgagca ctgcaaccat gacttcaaca acaaccatga ccctggccga
     2581 tctcaaggct caggaaaagg ccgccaagaa ggccgagaag gagcgtcaga agcgcatcga
     2641 cgccgacgag aaggccgagc gcaagcgcgc cgccaaaacc ggtttctcca acccggcgaa
     2701 cccgacccgc tcgatggccc tgaccatcgt gtgcgcgatt ttcgcggtct actgcctctt
     2761 cccgtttgtc tacctgatga ttaacgccac caagacccag tccgacttca cttcgacgtt
     2821 cggcctcggt ttcggcaaaa cgttcgccct gttcgacaac atcgccacgg tgttcaccta
     2881 tcaggacggt atcttcggcc gctggttcgt caacacgatt ctgtatgtgg tggtcggcgc
     2941 cggcggtgcc accttgctgg ccatcatggg cggctatgcg ctggccaagt tccgcttccc
     3001 cggccgtaag ctgtgcttcg tgatcatcat cggcgccatt tcggtgccgg gtatcgcgct
     3061 ggccgtgccg cagttcctgc tgttcgccaa gatgggcctc acgaacaccc cgtggtcgat
     3121 gattatcccg agtttggtat ccacgttcgg tctgtatctg atgtggatct tctccgatca
     3181 ggccgtgccg gatgagctgc ttgaagccgc gcgcgtggat ggtgccggcg agttccgcac
     3241 cttcttccag atttccctgc cgctgttggc acccggcatc gtgaccactg cattgttcgc
     3301 cattgtagcg acgtggaaca actacttcct gccgttgatc atgttgaagg attccaactg
     3361 gtacccgttg acgatcggtc tgaaccagtg gaaggaccag gcttcaaccg caggcggtca
     3421 agctatccaa aacctcgtga ttaccggttc gctgatcacg atcatccctc tggtgattgc
     3481 gttcctgtgc ctgcagaagt actggcagtc aggcctcgcc gcaggcgccg tcaaggagta
     3541 acctctggct ccctctgacg ggagctggcc gcggagcgga ctgagggtgg tcataggggg
     3601 attcgactgg cgaccacccc cagtcagctt cgctgacagc cccgccagcg ggggcggcct
     3661 ctctggtgtt cacaacctgc aagcacagca cttcccgcaa accgatacaa taaaggattt
     3721 gattaatatg actactcata gagcatttag gtggccgtcc ctgctgacgg aatccggccg
     3781 cggcatcgcg ttcggcggcg actacaaccc ggaccagtgg cccgaggaga cgctggacga
     3841 ggatatccgt ctcatgggtg aggccggcgt gaacgtcgta tcgctggcaa ttttcagctg
     3901 ggacaagatc gagccggtcg agggcgcgtt cacgttcgag tggctcgacc atgtgatcga
     3961 caggctcggc agagccggca tcgccgtgga cttggcctcc gccacagccg ccgcgccgct
     4021 gtggctgtac gaatcccatc cagaggtgct gccggtagac cggtatggtc ataccgtcaa
     4081 cgcgggctca cgccagtcgt ggcagcccac aagcccggtg ttcaaggaat acgcgctgcg
     4141 cctgtgccgc aaacttgccg aacactacaa ggacaacccg tatgtgaccg cctggcacat
     4201 gggcaacgaa tacggctgga acaaccgcta cgactactcc gacaacgcct tggccgcgtt
     4261 ccgcacgtgg tgcgaggcca agtacggtac catcgacgcg ttgaacgagg cgtggggcac
     4321 ggccttctgg tcgcagcacg tgaacagttt cgacgaggtg ctgctgccac gtcacatggg
     4381 cggcgacgcc atggtcaacc cgtcgcagca gttggattac gagcggttcg gcaacgacat
     4441 gctgctcgac ttctacaaag ccgaacgcga cgccatcgag cagatctgcc ccgacaagcc
     4501 gttcaccacg aacttcatgg tctccaccga ccagtgcgtc atgaactacg ccaaatgggc
     4561 ggatgaggtg gacttcgtgt ccaacgatca ctacttccat gagggcgaat cccacctgga
     4621 cgaactcgcc tgctcggacg cgctcatgga ctccctcgcg ctgggcaagc cgtggtacgt
     4681 gatggagcac tccacctcag ccgtgcagtg gaagccgctg aacacacgca aacgggccgg
     4741 cgaactcatg cgcgactccc tggcccacgt ggccatgggc gccgacgcca tctgcttttt
     4801 ccaatggcgg caatccaagt cgggcgccga ggcgttccac tccgccatgc tgccccacgc
     4861 gggcgccgat tccaaggtct tccgcggcgt atgcgagctc ggtaaggcgc tgaagaccct
     4921 ctccgacgcc gggctgcagg gcactgagct cgagcgtgcc ggcacggcga tcctgttcag
     4981 tgcggaatcc gaatgggcca cacgctccga aaccctgccc agcatgaaac tcaaccactg
     5041 gcacgacgtg cgcgactggt accgcggctt cctggatgcc gggctgcgcg cggacgtggt
     5101 gccgctcgcc tacgactgga ccggctacaa aaccatcgtg ctgcccaccg tgctcagcct
     5161 gtccgacgaa gacgttctcc gtatcgccga cttcgcgaag gccggcggaa ccgtcatcgt
     5221 cggctatgcc gccggcctga tcgacgagca cttccacatc ggcctcggcg gctaccccgg
     5281 cgccggcaac gggctcctac gcgacatgct cggcatccgc tccgaagaat tcaacatcct
     5341 cggtgaagaa gccgaaggcg agcccagcga aatcagcctg tccaatggtc tgacgacccg
     5401 cctgtggcag aacgacgtga cttccgtagc cgcggacacc acagtgcttg cctcttacgc
     5461 aggtgagtcc gccgccgact gggagctgga gcgcaccccc gccatcacca gccgccccta
     5521 tggcaacggt accgccatct acgtgggctg cgacctcaac cgtcacgaca ttgcccagtt
     5581 gctcaaagca cttggctccc gatggcagga gttgtcggcg cagccgactg agagtggtca
     5641 aactccaacc tatcccacca cagatccccg catcctgcac accatccgcc gatccgcaga
     5701 cggctccacc cgcttcgatt tctacctcaa ccgctcaaac cagcccgtcg ccatcaacgg
     5761 tgtggaaggt gatcccatca tcgcccaccg ttgcgagacg gatgccgtcg gatatacgct
     5821 gaaccgtaac gctattctta tcgctaaaac gtcctgttag accgcgctag aatggcaatg
     5881 ttgtccggat agaggagtgc ggcatgagcg acgagaaaac agccaagcgt cgagtgacgc
     5941 tacgcgatgt cgctcaggcc gccggtgtat cgctcaaaac tgcgtcgaac gtgatcaatg
     6001 gctccgggcg tatgacagat gagacccgtg ccaaggtcga gtcggtcatt aaggacctcg
     6061 actaccgagt caacgtggcc gcccgcaacc tcaaccgcga tcacaccggc ttcatcaccc
     6121 tcgccgtgcc ttcgctgatt ccgccatatc tggccgagct cgccaaccgc accatcgagg
     6181 ccgcccgtca gcgcgactat tccgtatacg tcaccacgta tgcggaaggc tccgcgaaag
     6241 gcgcgcgcga tctgctgaag aagttcaact ccacggtgtc cgatggcatg atcctttcca
     6301 tgagcgaggt ggaaaacatt tcgcccgatg atctgaaggt tgattttccg ctggtcatcg
     6361 ttggcgcgcg taccacgtgg ggcattgtgg atcacgtgac cccagacgat gtatccgcag
     6421 ccgccatggc cgcaggctac ctgtatgacc gcggttccac cagacttgca gtgatcggtg
     6481 cccgtgacgt ttacgacgaa gatgcattgc tgaatgccgt agaaggcaac gcgcagctgc
     6541 gtctgcgcgg catcatcgaa gagacccgta ggcgtgggct ctccttggat ccgcagctga
     6601 ttggcaacac cgatcaggat tggaccatcg gagccggtgc ccgtgtgacc cagcggctca
     6661 ttgattccgg cgtgccattc gacggtgtga ttgccttgaa cgatcagttg gccatcggcg
     6721 cattgaccgc tctgcgcacc gctggttatg aggtgcccgt gcaggtgcag gtcatcggct
     6781 tcgataacaa cgaggaagcc ccctacctgc agattccgct gaccaccatg gattcccgcc
     6841 ttgactggac ggctcccacg gcagtgaatc gcatcctcgg acgcatcgac ggcagcatca
     6901 ccaagtccga actgctcacc accgaatccc aggtcatcgc ccgcgcctcc acacgctgaa
     6961 cgacattgct gtaatgctgg tattgctgta ctgggccgta cgaggcgttg atacaccggt
     7021 tgaccaacac ggcataaagt gctaaggtga cgattgcgtg gtacacagct gtaccgcgtg
     7081 gcgcataagg aaaggaagcg ccaagcatca ctgggcgttc gttctgagcg ttcaccattc
     7141 aaaggagaaa aagcatgcgc cgaaataccg cgcttactcg cattatggca tccggcgtgg
     7201 ccgccataat gctctgcgca ggaggcacgt tcactgtaaa cgccgctgaa gaagaaccag
     7261 ttaaagccga cgtctcggtc aaggccatcc aaggcttaag cgatgatttc atcggtggta
     7321 tggacgtctc atccatgctc tcgctggaag aaagcggcgt cacgttcaag aacgccaatg
     7381 gcgaggtcga ggatttgttc acgttgctca aggaatccgg cgtgaactat gtgcgtctgc
     7441 gtgtgtggaa cgatccgttc accgcagacg gccaaggata tggcggcggc aacgtcaacg
     7501 ccgatcgcgc actgaccatg gccaagcgag ccacggcagc agggctgaaa gtgctggtcg
     7561 acttccatta ctccgatttc tgggccgacc ccagcaagca gcaggtgccc aaggcgtgga
     7621 aatcctttga aggcgacgcg gacaaaaccg ccgacaccgt atacgactac accaagcaga
     7681 ctctgaccac gttcaaacaa gccggcgtgg acgtcggcat ggtgcaggtc ggcaacgaaa
     7741 ccactgcgaa aatcgccggc atctccggct gggacggcat gtccaaagta ttctccgcag
     7801 gttccaaagc catacgcgaa gtgctccccg aagccaaggt cgtcattcat ttcaccaacc
     7861 cggagaaagc cggcacctac gccacttacg ccaaacagtt gagcaaccac aatgtggatt
     7921 acgatgtgtt cgccagctcc tactacccgt tctggcatgg caccaccgaa aacctcacca
     7981 gcgtgctcaa gaacgtggcc tccacttata agaaagatgt gatggtggcg gaaacgtcgt
     8041 gggcatacac cttggatgat ggcgacgacg attccaacac agtgcccagt aaagtaaccg
     8101 ccgataacct caagaaatac gacatcagcc cgcaaggcca ggccgatgag attcgcgcgg
     8161 tcgccgaggc cgtgaacaat atcggcgaca atgatggcga tggcgaaaac gacggcctgg
     8221 gtgtgttcta ctgggagccc gcatgggtgc ccgtgggcac cggcggcaag gataacgccg
     8281 aactggtgga tacgtggaac aagtacggtg gcggctgggc cactgaagcg gccggcgaat
     8341 acgatcccaa tgacgccggc ctgtactggg gcggctcagg tgtcgataac caagctttgt
     8401 tcgatttcga tggcaaggcg ctcgcctctc tgccgacgtt caagtacatt cacaccggtg
     8461 ccgtcaccga tcatgtgttc accaagatag atccagtgga gattaccgcg actgactcgg
     8521 attccatcga cgcgatcaaa gcgcaattgc cgagtgaggt ggccgcacac tatcaggatg
     8581 gggtggacga gaccgaaacc gtgacctggc aatccgctgc gcttgactgg attcgtggcg
     8641 caggcacata caccatcacc ggcaccacca acgctggcca cgacgtgacc gtcaccgtca
     8701 ccgtcaccgc cactcccgcc aaggattacg taacggacgg cagcttcgag aacgctgaga
     8761 acgataagaa ctggaccatt gccggcaccg gtgcgtccat caccgaagac agtggcaacg
     8821 ccgccgacgg caagcgcgcg ctgaagtttt gggcatccga tgcctacagt ttctccgcca
     8881 cgcagaccat caccggactc gagcctggcg aatacgtgct gaccgccatg agccaaggcg
     8941 cggcagcgga caacgccgct attaccgatg gtgtggccct ctccgccaca acgggtggca
     9001 agaccacgtc cgatgcgctg gaactcaacg gttgggttaa gttcgatacc gccaccgtgc
     9061 ctgtcaccgt gggtgctgat ggcacagcga ccatcaccat cacgggcaac ctgcctgccg
     9121 acgcatgggg caatgtggac aaggtctcac tcgtcaaaaa gaccgaaact ccggtgaagc
     9181 catccacgga gaacctggac aaggccgtgg ccgaagccgg gaaaatcaat cgcgatgagt
     9241 acaccaacga gtcgctcgcc aaactcgatc aggcgcttgc cgccgctgac gtgctgcttg
     9301 ccggcagcac ctacaccgag caggacgtga acgatgtgat caagcttgtt gccgatgcca
     9361 ttgccggcct cgcccaaaag gaggtctcca gcctgaccgt cacgccaagc aaaaccacct
     9421 atcaggtggg cgatgccatc gatgccgacc atgatctcaa ggtcgtgggt aactattccg
     9481 caggcatggg caacgtgacg ctttccgccg accagttcac gctcgattac gatttctccg
     9541 ctcccgctga tgcggcgaag gttaccgtaa cgctcaagtc gaatccgaat gtcaccgaaa
     9601 cctatacggt tgcggttacc gctcgggccg aaggtggttc cggcaacggc tcggacggtg
     9661 cgggtaatgg cggggctact atcaatcccg atacgggcga gggtgataag accaacggtg
     9721 cgaatggcga caagatcact ggtgtgctga gcaataccgg tagcgcggtg actgccgtgg
     9781 gtcttgcggt tgtcgttctc ggcgtggccg gcggggtctc gcttgctttg cgtcgtaagc
     9841 gctcctgata gagactgatg cggcattatg atgcggttgc cggcagccgg caaccggctg
     9901 ccagatgcca tctgtcggct gtcgtttatc ggttgtcggc tgtcagatgc gtctgtctcg
     9961 gcgcagcgat gcgattctgt cccggttcgt gcatcaatcg tttgatgtgg gtaccaggac
    10021 agaaatcact gtcctggttt gcgaaaacgg gtgtttttcg cgtaccggga caattcccgt
    10081 tgccccggtt tgcacgtcaa tcgttctacg tgagaaccgg gacagtatgt tgatttcggg
    10141 attggctgta ttccgccgat tctgtttgtc ggggtatgtg gactttgggg attgtcttgt
    10201 cctggttctc gaaaatggcc cgaaattgcg taccgggaca acgatttctg ccccggtgca
    10261 aacgtcaatc gattgacgcc tgtaccgggg caaattcgcg cccgggaaca atagggcgag
    10321 ggccggtcgc aggagtagag cgtgatgttg tcatgattgc gcccggctct cccacacgga
    10381 aggccgggcg caatcatgcc gtttggttgc agcacccgca catcgcctgc ataaccgtgt
    10441 acagtggcgt gtagttgcag tacatgaagg agcatcacaa gccatgacca acggacacat
    10501 caatcccgcc aacgtgccgg acatcgcctt gaaagacggc cattccattc cgcaggtcgg
    10561 ccttggcgtg ctacgtatcg atgacgaggg cgtggcccct gtcgtcgaat ctgcgctgga
    10621 agccggatac cgccacatcg atggcgccgc cggatacaac aacgaggccg gcgtgggtcg
    10681 tgcgctcgcc gcttccggat acaacaccgg cgccaagcgc gagaccctgt gggtcaccac
    10741 caagctgcgc gactccgagc agggctacga ctccgcgctc aaggcattcg acaactccct
    10801 gaaactcctc cagctcgatt acgtggacat gtacatgatc cattggccca cgccgttcga
    10861 ctggcgcagc accgacacct ggaaggcgtt cgtcaagctg cgcgacgaag gcatggcacg
    10921 cacgctcggc gtatgcaact tcatgcccgc cgatctgaag cgtctgcacg gggagaccgg
    10981 cgcatggccg gccgtcaatc agattgagct gcaccccacg tggcagcagc gcgaagtggt
    11041 ggcgttctgc aagaagcacg gcatcgccgt ggaggcctac tcgccgatgg cgcgtggcgc
    11101 cgacctcaac gccggcgacg gcaccatcga gaagatcgcc gccgcccatg aggtcagccc
    11161 ggcccaggtg attctgcgct ggcacatcga aaacggcacg atcatcatcc ccaagtccgt
    11221 gcatgccgac cgtcagaggg agaatctcga cctgttcggc ttcgccctga ccgccgatga
    11281 acacgctgcc atcgacgccc tcgacggccc cacccgcgcc ggccacgacc ccctcacctt
    11341 cacctatgcg tgattgatgg cacgtcgtaa cgcccacttc cgccgtcgtt tcaacgcctc
    11401 tggaccgctg gagcgcgtgc tgattctcat cgttctcgcc gtagcaatcg gcgtgaccat
    11461 cggcctgtta ctgccgcagg tcagctccga cgtggccaaa atcaccggtg gctacactgc
    11521 caccggttca gccgccgaca ctttgaacgc gctcgccgta gacgacaacc aaagctcctc
    11581 cggctacgac cgcgattcct tcggtttccg caccaccgac gtcgacggca atggctgcga
    11641 cgtacgcgac gacgtgctcg cccgcgatct cacggacatc acctacaaat acgccggttc
    11701 ctgtgtggtc gagtccggca cactggccga tccgtacacc gcccagacca tccatttcgt
    11761 acgtggccgc gccaccagcg ccaaagtgca gatcgaccat gtggtggccc ttgaaaacgc
    11821 ttggcaatcc ggtgcgcacg actggtccac tgccaaacgc catgaattcg gcaatgaccc
    11881 ctataacctg ctggccgtcg acggtccggc caatcaggag aagggttcgg cttccgccgc
    11941 ctactggcta cccaccaacg ccgactaccg ttgcgactac gtggcccgcc agattggcgt
    12001 caaagacaag taccagctca ccgtcacctc ccaagaaaag gacgccatgc tcgccgtcct
    12061 ccatacctgc ccgggccagg ccgtgcccgc agacgaatag
//
