LOCUS       CP014225               11852 bp    DNA     linear   BCT 02-FEB-2016
DEFINITION  Escherichia coli str. K-12 substr. MG1655, complete genome.
ACCESSION   CP014225
VERSION     CP014225.1
DBLINK      BioProject: PRJNA308372
            BioSample: SAMN04403366
KEYWORDS    .
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Kurylo,C.M., Alexander,N., Dass,R.A., Parks,M.M., Altman,R.A.,
            Vincent,C.T., Mason,C.E. and Blanchard,S.C.
  TITLE     Genome sequence and analysis of Escherichia coli MRE600, a
            colicinogenic, nonmotile strain that lacks RNase I and the type I
            methyltransferase, EcoKI
  JOURNAL   Genome Biol Evol (2016) In press
   PUBMED   26802429
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Kurylo,C.M., Alexander,N., Dass,R.A., Parks,M.M., Altman,R.A.,
            Vincent,C.T. and Blanchard,S.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2016) Physiology and Biophysics, Weill Cornell
            Medical College, 1300 York Ave., New York, NY 10065, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP2 v. 2.1.0
            Genome Coverage                   :: 125X
            Sequencing Technology             :: Pacific Biosciences
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/02/2016 10:49:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 3.1
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 4,617
            CDS (total)                       :: 4,489
            Genes (coding)                    :: 4,407
            CDS (coding)                      :: 4,407
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 41 of 82
            Pseudo Genes (incomplete)         :: 34 of 82
            Pseudo Genes (internal stop)      :: 23 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            Annotation Pipeline
            set; GeneMarkS+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:47076"
                     /db_xref="taxon:511145"
     gene            complement(1..693)
                     /gene="ompL"
                     /locus_tag="AW869_11135"
     CDS             complement(1..693)
                     /gene="ompL"
                     /locus_tag="AW869_11135"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001309878.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P76773|1.B.35.2.2"
                     /protein_id="AMC96743.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVGY
                     NFDMGAGIMLTNTYNFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGSGG
                     AVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKFSYT
                     FEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHREQNQI
                     RIGTKYFF"
     gene            complement(761..2164)
                     /locus_tag="AW869_11140"
     CDS             complement(761..2164)
                     /locus_tag="AW869_11140"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001279690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P32137|2.A.2.3.9"
                     /protein_id="AMC96744.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDEL
                     GMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIATLQ
                     FIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQGGAT
                     IGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTPTGHK
                     ASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWMGFFSM
                     GCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCVAFFGTA
                     FVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIMLTQIGYVP
                     NIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQRKNKEMAT
                     EEKTASVTL"
     gene            complement(2207..3592)
                     /locus_tag="AW869_11145"
     CDS             complement(2207..3592)
                     /locus_tag="AW869_11145"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000018363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P32137|2.A.2.3.9"
                     /protein_id="AMC96745.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFYT
                     DVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTLLA
                     IANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAWRQG
                     GATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYVETQP
                     ANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDPILLSY
                     MGFFSMGCIFIGVFLMPASVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSFVAFSCL
                     AFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGFFPGWMLT
                     QIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRIVEEIEARK
                     RTA"
     gene            complement(3638..5674)
                     /locus_tag="AW869_11150"
     CDS             complement(3638..5674)
                     /locus_tag="AW869_11150"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005121834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH31"
                     /protein_id="AMC96746.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSGI
                     ADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGRLL
                     LELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYV
                     TWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEDKA
                     TLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVK
                     VNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYINPYVAS
                     DKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGC
                     GGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEILFFMRAGS
                     TGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRS
                     KELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFTTLKPYLKEAV
                     ALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNW
                     VHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            complement(5873..6775)
                     /locus_tag="AW869_11155"
     CDS             complement(5873..6775)
                     /locus_tag="AW869_11155"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005052848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="AMC96747.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIPH
                     KPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQISE
                     LTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGIHPY
                     LTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDHTFKT
                     ANDLWEMTITHPQQALSVSLCSDQLWVQVYSGEKLQRQGLAVEPMSCPPNAFNSGIDLL
                     LLESGKPHRLFFNIYGQRK"
     gene            complement(6913..8154)
                     /locus_tag="AW869_11160"
     CDS             complement(6913..8154)
                     /locus_tag="AW869_11160"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000870897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="AMC96748.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKEE
                     MGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEGVV
                     DASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDEAFS
                     KTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNEHFDT
                     QWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLEDAKGL
                     FNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGDRQYETW
                     YQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPRIPLAPGM
                     APAVAAGLLDINAK"
     gene            complement(8171..9049)
                     /locus_tag="AW869_11165"
     CDS             complement(8171..9049)
                     /locus_tag="AW869_11165"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001737627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="AMC96749.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSVL
                     TDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVDSV
                     VIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEPVVR
                     PPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHINMPW
                     VILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPKLQQLG
                     DIVDEMMAKRR"
     gene            complement(9073..9969)
                     /locus_tag="AW869_11170"
     CDS             complement(9073..9969)
                     /locus_tag="AW869_11170"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001406350.1"
                     /note="catalyzes the formation of gamma hydroxybutyrate
                     from succinate semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-hydroxybutyrate dehydrogenase"
                     /protein_id="AMC96750.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGA
                     TPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQTDK
                     LIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINAGG
                     PGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPN
                     KVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSA
                     ILEQVRVSAGMTAKVKM"
     gene            10137..11033
                     /locus_tag="AW869_11175"
     CDS             10137..11033
                     /locus_tag="AW869_11175"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P32143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STP|PfkB"
                     /protein_id="AMC96751.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATAA
                     VAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTKGE
                     RIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLDGDI
                     TPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGCDWLE
                     NGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCTRPGGR
                     AGIPDCDQTRSFLSLFV"
     gene            11067..11852
                     /locus_tag="AW869_11180"
     CDS             11067..11852
                     /locus_tag="AW869_11180"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001612094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_DeoR,DBD-SUPERFAMILY|0043758"
                     /protein_id="AMC96752.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRDI
                     RKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFITI
                     GTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPSAAS
                     FVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHASAAV
                     EIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
ORIGIN
        1 tcagaagaaa tacttcgtcc cgatacggat ctggttttgc tcgcggtggt acggttcgac
       61 gttgcggtct aaccagcgca attcgaaata agggagccag tgttcattaa tacggtaacg
      121 gaaggtgtta gtgatttccc aatgatgatc tttcccgttg ctactattaa agtcattcac
      181 tcgcatgaag taatgtggct caaatgtata ggaaaattta tcggtgattt taaaattcca
      241 gtaggtgcca atttcatagg tgtcattatt atccagttcc ccgctcaaat ctgttgaact
      301 atagttatta tggttatagc gatttcgtac tgtcagatta aaccatggta caaatttata
      361 gttgacgtcc agatacactg caccacccga accgatactc ttatcattaa ttaagccacc
      421 gggctggatg gttaatttat cggttggttt aaataacgga taccagcctt caatttcgtt
      481 atatccatgt tttagttcat cttctcgctg aaagttgtag gtattggtta acataatacc
      541 cgcgcccata tcgaagttat aacccacacg tagcataacc tcgccctgat cggaggcaag
      601 attatatgcc tccctgtttt cgacgtatgc gccagcaaat accgaggcag aggtcagaga
      661 agataataaa attattgcat taatcttttt cataataagt ccatttttta aacgtaggga
      721 ggccgccacc caccaggatg gaaatagcgg ctattgacat ttataacgtt acggaagccg
      781 ttttttcttc tgtcgccatc tctttatttt tgcgttggtt gatttcctca ataatcaatg
      841 caaaccgttt ttcattgagt gtgtaaaaga atcccatcgt caatgcagca ataattgcca
      901 gtgcacaagg ccagataaaa atgagctgac gcaaaccctg taatgtcgca tcgctttgcg
      961 cgatgttggg aacataacca atttgcgtca gcataatgcc tggcaagaag ccagcaagtg
     1021 cggcagaaat tttacgaaag aaggtataac cggtataaac agaaccttcg gcacgaatac
     1081 cggttttcca ttcaccgtaa tctacggtat ctggtaccag tgcccagttc aggctgttta
     1141 caaaggccgt gccaaaaaag gcgacacaag agaacatgac gaaagtgaaa gagttacttc
     1201 cccagaaata attcagtata tcacccaccg cccacagcac catgccagcc agataaacct
     1261 gttttttacc aaaacatttt acagttaatg gcaccagtaa tacgccgatg aggatgcatc
     1321 ccatactgaa gaatcccatc cacgacaata aattaatatc attcagcaca tactgggtgt
     1381 aatagacctg aatcgccagt ttgatattaa atgccgccag ggtacacaag ttagcaatgc
     1441 ataaaaccag caatggcgga ttccgaaata tcgcgcaaaa tgatttgaga atactggctt
     1501 tatgtccggt tggcgttgtg tccacataat gctctttgac gcctctgtag cacagcatca
     1561 taaaaataaa gccgccaatg gagaacataa gtgccgcaca ggcataaccg acggttgagt
     1621 cagaaaaaag cgactgcaac ggaataaacg ccacggtaca aatcaatagc cctatggttg
     1681 caccaccctg gcggtaagcc gcgagctgcg cgcgctcatt cgggtttttg gtaattgccg
     1741 ggatcatcgc accatacgag cagttcatca gactgtatga aagtccaaac atcataaaca
     1801 gcgcggtggc aatcgtcgtt ttaaccggca aacaaaaggt ggtggcgata aactggagcg
     1861 ttgcaattaa cgccgccgga accgctgcat ataaaataaa gggacgaaat ttgcctttag
     1921 gcccaatatt tttccgcgag tcgagtaaaa atccggtgag catatcggta aacgcggtaa
     1981 aaaacttcgc caccagaaag ataatcccgc catagtaagc aggcatccct aattcatcgg
     2041 tataaaattt gaggagatac agagtaccga tgcagagcat taaattcgaa ccgacgtcgc
     2101 ccataccata ggcgattttt tcccgcagat tcagttttaa tgtcagtgga ttatggtcag
     2161 acataatcac tccttataac gccccgcagg gcgtcgttta ttattattac gccgtgcgtt
     2221 tacgggcttc tatctcttcc acaatgcgga catacatctt ctcgttcagg ctgtagaagc
     2281 aacccatcgc cacaatggtg actaccgcca gtgcgcttgg gtagatgaag atcaactggc
     2341 gtaacccttc aatggtgtgg tcagcctgtg cgacgttcgg cacatagcca atttgcgtca
     2401 gcatccagcc ggggaagaaa ccagccagcg cctgagacac tttgcgaaag aaggtaaaac
     2461 cggtgtagac cgttccttcc gaacgcacgc cggtgcgcca ctcgccgtac tcgacggtgt
     2521 cggaaaccag cgcccagttc aggctgttaa caaacgctga gccaaagaac gccaggcagg
     2581 agaacgccac gaagctgacc gaaccgccgc cgaagaaata gttgagcaga tcgcccagca
     2641 cccaaatcag caggccgccg atataaactt tcttcttgcc aaaacgtctg actgaggcag
     2701 gcatcaggaa tacgccgatg aaaatacagc ccatgctgaa aaatcccata tacgacaaca
     2761 ggatgggatc gttaagcacg tactgggtgt aatagacctg gatggcgagc ttgacgttaa
     2821 acgcccctaa agtgcagagg ttggcaatgc acagaatgaa cagcgggcgg ttacccgcaa
     2881 ttgcgcggaa agattgcagc aggcccggct tttgcgccgg attagcaggc tgggtttcga
     2941 cataacgctc tttcacgccc gagtagcaga tccacataaa cagcaggcca aacagtgaaa
     3001 acagcgtggc ggcgaagata tagccaagtt gctgattacc ttcgataaga ttcataactg
     3061 gcacgaatcc caccgtgcac agcagcaggc ccagcgtagc gccgccctga cgccatgccg
     3121 ccagtgaggc gcgttcgttg gggtttttgg tgatagcggg aaccattgcg ccgtaggagc
     3181 agttcatcat gctgaaaaac agtccgtaaa gcataaacag aatagtggcc atcaccgttt
     3241 taccggtgac atcaaacggt gtgccgacaa agttggcgat cgccagtaag gtgaccggga
     3301 atgacgcata cagaataaaa ggacggaact taccttttgg accgatcttg cgtcgggaat
     3361 ccaacataat gccggtaccc atatcggtaa acgcagtaaa gaattttgaa atcaaaaaga
     3421 taatgccgcc ataggtgcct ggcagcccca gaacgtcggt ataaaacttc aaaagataaa
     3481 gcgtaccgat atccagcagg atgttagagg ccaggtcgcc aataccgtaa gagagtttct
     3541 ctttaaaggg caggcgtaaa gttgctggat cttccgttgt gatgtgactc atcgatgttc
     3601 tccctttgcc catgagtttc cccacgggcg gatgtgatta gatgcttttt aacgacgcga
     3661 acagtgccgc ccattcgcta tcggcgcgat aaaagaccgg cggcttgccg atgggcgcat
     3721 taacggtaac ttccccgccc cggaacgctt cacccgtcca ggcgtggacc cagttatcct
     3781 ccggcagata gagcgtccag tcgctacggc cttcttcatg caccggagcg accagaatgt
     3841 cgcgacctaa caggtactga tatttcaggg tgtaagtgtg cgcatcgtct tcgtaatgca
     3901 ggaacagcgg gcgcataacc ggcaggccgg acttcgcatt cagcgcgacg gcctctttca
     3961 ggtaaggttt cagggtggtg aagacggtgg tcatacgggc gaaatgggcg atggtttctg
     4021 cgtcgccgtc aaactgccag ttgtcgccag gacggttacc ttcgtgggtg cgcatcatcg
     4081 gcgtgaaggc gctgaaatcg caccagcgca gcagcagctc tttgctgcgc ttcatctcaa
     4141 acagggtggt gtaaccgcca atgtcgctgt ggtgcaggcc atgtccggtc attgccagcg
     4201 acagcgccgc cgggacaacc gacgccaggc catcgtcgag actccagtcg acgttctggt
     4261 cgcccgccca catcatggtg gagtatttct ggctaccggt agaaccggcg cgcataaaga
     4321 aaaggatctc gccgagcttg cccgtttctt caagggcttc gtagttacac ttcgcccaca
     4381 gcgcaggcca ggcgttatgc ataatttcgg cactgacgcc gttatgcaag tacgtgtcgg
     4441 tgggcagata ctcgccgaag tcagccatcc agccgccgca gccgagttca atcatgttct
     4501 ttttgatcac ttccttgaac caggcgtagg cttctggatt agtgagatcg acaacgccgc
     4561 cgtaaaactc gccaaactcc accagatagt caccgccaga ggcatctttt gccagatagc
     4621 cgtgttgtgc cgcttcttcg cagagatctt tatcgctggc aacatacggg ttgatatagg
     4681 ccaggaactg cacgccctcc tgattccact gcttaatgcg tgaatccagt tgcgggtagt
     4741 tttcgctgtt ccacttccag ttccacatca cgcgtttgcc aaaagaggtc atacgaatac
     4801 cggaccagtc ctgcgcccag atgccgttga ccttcacgcc cgcgttacgc atggtgtcca
     4861 gtttcttctg gcacacttcc gtcccgccct gaatgccgag cgttactccg tcataaatcc
     4921 agtcgggcag ttctggctgg cgtcccagca gggcggttaa tttttccagc agggaaatgt
     4981 atgtgtcagc acattcaaaa cgcagcgttg ctttgtcttc ccacagcgcc agttcatggt
     5041 attccggggc actaaagtcg aagttcatat agcaactgtt atcaacatgg cagtaatact
     5101 tctgcgtgct gacaaacgta ggctgtggga agaaagtcca gtaatagtcg ccgcccgcat
     5161 tttctttgca gtcggcctgc caggtgacat aggtttgttt gttgcgacca acgccttgtt
     5221 cactggtcca tagcgggaac ggtttgccac gcagatcgaa gtaggaaaac tgttcgccgc
     5281 agccgtagat atggtcctct ggttgagcgg caaggcgcag ccagatacgg ttgtggttaa
     5341 ggttgtcgtt ttgtagttcc agcaataaac gcccctgatc gtcggcagag atattcagcg
     5401 tggcgctaat gtcagaacca cggctgaaat gaattaacca accatccggt gactggctga
     5461 cgatggcgtc ggtaagcgca attttctcct gtagtttatc tttaatgctg aaattaccgc
     5521 ggaacatatc gatatccgct atacctgagc caatccataa acaaggatta tctttgctat
     5581 gggttaaaat aagacgttgt tgaaaatgta gggtaaaact gtcgttattc tgatgaaatt
     5641 gaaaatctaa taactgtgga cgtggcgtat ccataactaa tctccataga gttgagaacg
     5701 agattgttgc cttcctgtta ttacaggctt atgtaaaatg attctcataa aactgatata
     5761 taaaaacttt acagaagctg agctgttttt aacttaataa attgaagata cataatgttg
     5821 cctttagcga gtgaaatgtt cttcaatgat tttgactgct aatcaataca agttattttc
     5881 tctgtccgta aatattaaaa aacaggcggt gcggttttcc tgattccagt aataataagt
     5941 cgatacctga attaaaggcg ttaggcgggc aactcatagg ttcaactgcc agtccctgac
     6001 gctgtaattt ttcaccgcta taaacctgca cccagagttg atcggaacaa aggcttaccg
     6061 aaagcgcctg ctgtggatgg gtaatggtca tttcccacaa atcattagcg gttttaaagg
     6121 tgtggtcgat ttttgtcgcg gctatttttt tcgcctgcgt gaaatttaaa tccagctcat
     6181 caacgtggtg aagcgttgtc ggattcgcgt gctcatcgac ggcataaacc tggtttgccg
     6241 gaagctggaa cagatattca tcaacagatg ttaagttgca ggttaaataa ggatgaatac
     6301 ccacgccata aggcgctgcg acagtgccga tattctggct tgcgatctca acggaaagac
     6361 ccgtgtgtgc gttgagcgaa taaacaactt gtgaagccag cataaacgga tagccgtaac
     6421 tgggcggcag aaaagctgtc agggtaacgc tggtggcggt taattcacta atttgccagt
     6481 ctcgccaggc aagcagcccg tgaatagcgg cttttgaact atgttcatta atgggtaact
     6541 gatattcctg cccctgatag cggtaacagc cgttggcgat ccggttcggc cacggaataa
     6601 gcactttacc cagatgcgcc agcggcatct cttcgggttt gtgcgggatg actaaatgac
     6661 aaccctgaaa ggttaattca gccagaccag caccgacggt cacaatggtc gcgtgataat
     6721 cccccgcggc aagtgaaacg gtctgtccac tgcaatgcat attggtgatt tgcatacaag
     6781 gcaccttaat taacgacata cagcctcccg tgaggctgat gtcaaaccca aaaactgcac
     6841 cggacggtcc cctcgcccct ttggggagag ggttagggag aggggaaaac ccgcacccta
     6901 ttttcagatc agttatttcg cattaatatc cagcagaccc gctgcaaccg ctggagccat
     6961 gccgggcgct aacgggatac gcgggatcac caggcaatgc agcaggtgat aaatatcctg
     7021 tttgccgtcc cagactttgg tggtgacctt attgtccgca tccagctcct gccaccagga
     7081 accattttca tagtccatca ggtacttaat gcagtactcc caccatgttt gataccaggt
     7141 ttcatactgg cgatcgccag tgacggtgta gagcgcgtag gccgtaccca ttgcttcgac
     7201 gataggccaa cgtacacgtt cgcggaccac cggttttcct tcccagtcaa cggtataaac
     7261 aatcccgtcc gcgccatcgg gtgcccaggc atcgcgcacg gtggcgttaa acagaccttt
     7321 ggcatcttct agcagccatg ctggtggttg ttcgcaacgg gcttccaggg cagcatggat
     7381 gtgcagcatt aaacggcccc attcgatcca gtggcctggt gtacctccga acgcgcggaa
     7441 gcgatgcgcc gggttatctt tgttgtaatc cggcagcgga ttccactggg tatcgaagtg
     7501 ttcgttaacg cgataatgat tatttctggc gacgtcgtgg ataatcacgg aagccacgcg
     7561 aatcgcgcga tccagccatt ttttgtcgtg agtgacgtca taaacaatca agaaagcttc
     7621 caccgcgtgc atattggcat tgccgccgcg gtactcttcg gttttgctga aggcttcgtc
     7681 ccaggattcc aggcacatct gctcttcttc gctccagaaa tatttctcga taatttcaat
     7741 ggtgtaatcg agcagcttgc gcgcttccgg gtgacccgtt gtgacggcgc tggcggcacc
     7801 cagcagagca aagaagtgtt gatagccctg tttggaggca tccaccacgc cctcgtcatt
     7861 cacgcaggca taccagccgc catatttttt atcacgcagt gcgccgttca tggctttgat
     7921 accgtgatca accaacgagt aagcgccagg tcgacccatc gccgcagcaa cggaataaac
     7981 gtgcaacata cgagcggtga tccacagatg ggtgcccatc tcttctttga tttgcccttt
     8041 attgcctaac cagccaaaac cagtcggcac tacggaattt ttaccaaaat caaagatgcg
     8101 gtcggtttcc tgttccagcc aacggttgtg gcttagggtg ttaaaccatt tcattccttt
     8161 tatcctcatc ttagcggcgt ttagccatca tttcgtcgac gatatcgcca agttgttgta
     8221 atttcggtgc gcaaacgtca cgcagcatca gctcgttgtc tggtaaaccg acgaccgatg
     8281 cccagactgc acggcctgcc aggaatcccg atgcgcccgc cgtcattgcc acgcgtacgg
     8341 cacgcgggaa cagtttttcg tcgacaccgg aagagaggat cacccatggc atattgatat
     8401 ggtcattcag acgttgtgaa gcacagagaa gctcttgttg cggacctttg ccataaagcg
     8461 gcatttcaac tttgtagagg tcagcgccac tgtcgcccag ctctttggcg gcatcgatga
     8521 tcgcttgttc gcgatcgaat ttatcgccac gacgcggtgg gcggacgact ggctcaatga
     8581 tgcttaccag accgtgtgag tggcacagtt cgttgaactc tttcaccata tccagacgtt
     8641 gctgcgcatc ttcatcacta cgccacagca ccagcagttt taaggctttg ccaccgtctt
     8701 gtttgatctg tagcggattg attttgcggt caataaccac gctatcgacc ggaataccgt
     8761 tgccaggaat gaactcatcg gcggcgacaa tcatggcgca acttttggca atcgcgtttt
     8821 gctcaaccac ctggcggtag cagaattgtt gatctaccag aatcgccgag gcataaggcg
     8881 agagggcctt tgcagcgtta actttgaaat cagttaaaac gctatcggct accggagcgg
     8941 gtgccccagc cgcggcaaac atcatgcgca tggcttcgcg ctgatcgacc gccagcatgg
     9001 caaaaccgcc cgatgcgcgc gtaatgtcgt tgatggtgta cttattcatt catttattcc
     9061 tttatccagt cgttacattt ttactttggc agtcatcccg gcactgacac ggacctgttc
     9121 cagaatggcg gaccagtcct ggcgaccgcg acccgctgcg cgcgcctggc tataaacctc
     9181 ccgtgaggcg gcccccagcg gcattggcac atgcagctgg ttggcgacat caagggcgat
     9241 gccaagatcc ttatgggcaa gatcgatcat gaaggcggga gaaagatctc cgctgaggac
     9301 tttgtttggc caggaagtgg tgaagtgacc tttaccggcg gcggtaccgc tcatcacttt
     9361 gacggcaaca tcgaagggaa gattcagggc ttcgcacaaa acggcggctt ctgccgaaag
     9421 cgcattgagc gcgatgctca tgtagttgtt gatgagctta acgcggatcc ccatgcccgg
     9481 accgccagcg ttgatcaact cactgcccat cgccatcagg atcggtgtgg cacgttcaac
     9541 ttgttcagcg gtgccgccag ccagcagtaa cagagtaccg gtaatggcat ttgcagaagt
     9601 acggcctacc ggaacatcca tcatgctgaa gcctttggct tgcatatcgg caatcaattt
     9661 atcggtttgc agcggatgga tggtggacat atcaatgacc agcgcatcgg tagataagcc
     9721 ttcgcaaacg ccgttttcac cgaacaacac gttgcgcacc agatcgccat tcggcaacat
     9781 ggtaatgata aattcggcat ctttagcggc ctgcgccggg ttggcggcgg gagtcgcacc
     9841 tttgtctacc agatgccgca cagcttcggc gttcacatca aagacgcgaa gttgatgccc
     9901 ttgctgcaat aaattgctcg ccattggcga acccatttgt cctaaaccga taaacgcgat
     9961 tgctgccata accctctcct gaatacagtt atgtcacttt ttgtcattta tgacatgctt
    10021 tgcttgtctg tttttgatcg tatttgtaat ttatcgtcaa aaaattgaca gccgtcactt
    10081 tttaaacaat tggtgaaatt aaaatgaacg catcccaaaa tgtttaagga atgaccatga
    10141 ttcgtgttgc ttgtgtaggt ataaccgtga tggatcgcat ctattacgtg gaagggttac
    10201 cgacggagag cggtaaatac gtggcgagaa attatacgga agttggtggc gggccagcgg
    10261 cgacggcagc ggttgcggcg gcaaggctgg gggcgcaggt cgattttatt ggtcgcgtag
    10321 gtgatgacga cacgggcaac agcctgctgg cagagctgga atcctggggc gttaacaccc
    10381 gttacaccaa acggtataac caggcgaaat cttcgcaatc cgccatcatg gtggatacca
    10441 aaggcgagcg gataatcatt aactacccca gcccggatct gctgcctgac gcagagtggt
    10501 tggaggaaat tgatttctct cagtgggatg ttgtgctggc agatgtacgc tggcacgacg
    10561 gcgctaaaaa agccttcacc ctggcccgtc aggcgggtgt gatgaccgtt ctggacgggg
    10621 acattacgcc gcaggatatc agtgagctgg tggcattaag cgatcacgcg gccttttcag
    10681 aaccgggtct ggcgcgctta acgggcgtga aagagatggc cagtgcgcta aaacaggcac
    10741 aaacgctcac aaatggtcat gtctatgtga cccagggtag cgcaggctgc gactggctgg
    10801 aaaatggtgg gcgtcagcat caaccggcct tcaaagttga tgtggtagat accacaggtg
    10861 cgggtgatgt ttttcacggc gctttggcgg tggcgctggc aacaagtggg gatttagcgg
    10921 agtcagtccg cttcgccagc ggtgtagcgg cgttaaaatg cacacgtccc ggtggacgag
    10981 ccgggatccc tgactgtgat caaacccgat cttttttgtc actttttgta taaaatgcca
    11041 gggtgatggt ttttcgagga attttcatga gccttaccga actaaccggt aacccgcggc
    11101 acgaccaact cctcatgctg atcgccgagc gtgggtatat gaatattgat gagctggcaa
    11161 atctgctgga tgtctccacg cagacggtcc gccgggatat tcgtaaatta agcgagcaag
    11221 gcctgattac gcgccatcac ggtggcgcgg gtcgggcttc cagcgtcgtt aatacggcgt
    11281 tcgagcagcg tgaggtttcg caaaccgagg aaaaaaaagc gattgccgaa gcggtggcag
    11341 actatattcc tgatggttca acaatattta tcaccattgg tacgactgtt gagcatgttg
    11401 cccgggcgtt acttaaccat aatcatttgc ggataatcac caacagcctg cgtgtggcgc
    11461 atattcttta ccacaacccg cgctttgaag tgatggtgcc cggcggtacg ttgcgctctc
    11521 ataatagcgg gatcattggc ccttcagcgg cgtcctttgt ggctgatttt cgtgctgatt
    11581 atctggtaac aagcgttggg gcgattgaga gcgatggcgc gttgatggag tttgatgtaa
    11641 acgaagctaa cgtggtgaaa acgatgatgg cgcacgcgag aaatattctg ctggtcgccg
    11701 atcacactaa gtatcatgct tcggcggcgg ttgaaattgg taacgtggca caggtcactg
    11761 cgctctttac cgacgagctg ccgcccgctg cgctaaaatc acgcttacaa gacagccaaa
    11821 ttgaaatcat tcttccccag gaagacgcgt ag
//
