LOCUS       CP000303                7061 bp    DNA     linear   BCT 31-JAN-2014
DEFINITION  Bifidobacterium breve UCC2003, complete genome.
ACCESSION   CP000303
VERSION     CP000303.1
DBLINK      BioProject: PRJNA13487
            BioSample: SAMN02604112
KEYWORDS    .
SOURCE      Bifidobacterium breve UCC2003
  ORGANISM  Bifidobacterium breve UCC2003
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   O'Connell Motherway,M., Zomer,A., Leahy,S.C., Reunanen,J.,
            Bottacini,F., Claesson,M.J., O'Brien,F., Flynn,K., Casey,P.G.,
            Moreno Munoz,J.A., Kearney,B., Houston,A.M., O'Mahony,C.,
            Higgins,D.G., Shanahan,F., Palva,A., de Vos,W.M., Fitzgerald,G.F.,
            Ventura,M., O'Toole,P.W. and van Sinderen,D.
  TITLE     Functional genome analysis of Bifidobacterium breve UCC2003 reveals
            type IVb tight adherence (Tad) pili as an essential and conserved
            host-colonization factor
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2011) In press
   PUBMED   21690406
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   O'Connell Motherway,M., Zomer,A., Leahy,S., Reunanen,J.,
            Bottacini,F., Claesson,M., O'Brien,F., O'Flynn,K., Casey,P.,
            Moreno-Munoz,J., Higgins,D.G., Shanahan,F., de Vos,W.,
            Fitzgerald,G.F., Ventura,M., O'Toole,P. and van Sinderen,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2006) Department of Microbiology, Alimentary
            Pharmabiotic Centre, University College Cork, College Road, Cork,
            Munster, Ireland
COMMENT     Source DNA or strain is available from Douwe van Sinderen,
            d.vansinderen@ucc.ie.
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Bifidobacterium breve UCC2003"
                     /mol_type="genomic DNA"
                     /strain="UCC2003"
                     /db_xref="taxon:326426"
     gene            <1..8
                     /locus_tag="Bbr_0525"
     CDS             <1..8
                     /locus_tag="Bbr_0525"
                     /EC_number="4.2.1.70"
                     /codon_start=2
                     /transl_table=11
                     /product="Ribosomal large subunit pseudouridine synthase D"
                     /protein_id="ABE95222.1"
                     /translation="MTWISPATSPSPSPLSKPHRYVIDEPHIPFEMDVLYEDERIIVVD
                     KPHFLATMPRGMWYRETALIRLRERLGEPDITPAHRLDRMTAGVLVFVRDPACRGAYQM
                     LFQNKQATKVYECLASCAPIRRPRFGTITQAEPPRPFPLLRRSHITKERGTLAAFEEPG
                     AVNAETLIERGELILTSAGGRWREAPEGGPCLENPPICRYTLHPRTGKTHQLRVHMNSL
                     GLPIVGDDFYPRIQTRLYDDFSQPLQLVARVLRFTDPVTGKRQEFVSRIPLIS"
     gene            complement(1..1011)
                     /locus_tag="Bbr_0526"
     CDS             complement(1..1011)
                     /locus_tag="Bbr_0526"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="ABE95223.1"
                     /translation="MARPTQVSMQDVAKAAGVSPQTVSRVANDSNAVKPETRQRVEAAM
                     ERLGYRPNYAARALKHGRFQDIGVVMFDTTRFGNARILDSIVTHAAEDGYSVTIQTMSN
                     GAERTLASAIDRMQRQPVDGVIVVLEERISDFVGYKPPRELPVVLISEAAADHCPTVDA
                     DQYGCSTAIVDYLMSKGHKTVYHIAGPVTSRAAESRAQGWREALEQIGARVPSMYIGDW
                     CADSGYQAGVALAHDPECTAIYAANDQMAYGAMLGLESAGKRVPEDVSIVGVDDSLVDV
                     VPRLNLTTMRMRNDAIGATAFSMVRRQCEGEKVPAGIKTVIPTELIKRGSVRDIS"
     gene            1227..2153
                     /locus_tag="Bbr_0527"
     CDS             1227..2153
                     /locus_tag="Bbr_0527"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|D6ZW63|3.A.1.1.48"
                     /protein_id="ABE95224.1"
                     /translation="MASADVAAVPMHTDKARENKHKADWRGWKFMWPFTVVFVFVFIIP
                     IVYAVYISFFKKQMIGGTRFVGVENYARLLQDGQFWSSVGRVALFTCVQVPIMLFLSAA
                     MALALDSMKLHGAKFFRISTFLPYAVPAVVSTLVWGFVYGAKYGLVGSFNDFFGTNVDV
                     LQPGVLLASIGNISTWEFTGYNMLIFYSSLSTIPHSLYEAASIDGASEWQIVKSIKLPE
                     LKGSLAITVIFSIIGSFQLFNEPSILQNMVPGNAITTYYTPNMYAYNLSFSGNQSNYAA
                     ALAIVMAVITMAIAYAVQLKSMKEQMK"
     gene            2206..3204
                     /locus_tag="Bbr_0528"
     CDS             2206..3204
                     /locus_tag="Bbr_0528"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|D6ZW62|3.A.1.1.48"
                     /protein_id="ABE95225.1"
                     /translation="MSAAASTRLTDAELKAREKAARKAEKERQKRIAADEAAERKRSAR
                     AGFANVNNPRRSTLMTVLCAIFAVYCLFPFVYLVINATKTQADFTSTFGLGFGKSFALW
                     DNIVTVFTYQGGIFGRWLVNTLLYVVVGAGGATLLAIMGGYALAKFRFPGRKAVFAVII
                     GSISVPGIALAVPQFLLFAKLGLTNTPWAMIIPSLISPFGLYLMWIFSEQAVPTELLEA
                     ARVDGASEFRTFWTISLPLLAPGIVTTALFTIVATWNNYFLPLIMLKDADWYPLTIGLN
                     QWKDQASTAGGQAIQNLVITGSLITIIPLVIAFLCLQKYWQSGLAAGAVKE"
     gene            3384..5459
                     /gene="lacZ5"
                     /locus_tag="Bbr_0529"
     CDS             3384..5459
                     /gene="lacZ5"
                     /locus_tag="Bbr_0529"
                     /EC_number="3.2.1.23"
                     /codon_start=1
                     /transl_table=11
                     /product="GH42"
                     /protein_id="ABE95226.1"
                     /translation="MEHREFKWPQPLAGGKPRIWYGGDYNPDQWPEEVWDEDVALMQKA
                     GVNLVSVAIFSWAKLEPEEGVYDFDWLDRVIDKLGKAGIAVDLASGTASPPMWMTQAHP
                     EILWVDYRGDVCQPGARQHWRATSPVFLDYALNLCRKMAEHYKDNPYVVSWHVSNEYGC
                     HNRFDYSEDAERAFQKWCEKKYGTIDAVNDAWGTAFWAQRMNNFSEIIPPRFIGDGNFM
                     NPGKLLDWKRFSSDALLDFYKAERDALLEIAPKPQTTNFMVSAGGAGIDYDKWGYDVDF
                     VSNDHYFTPGEAHFDELAYSASLCDGIARKNPWFLMEHSSSAVNWRPINYRVEPGELVR
                     DSLAHLAMGADAICYFQWRQSKAGAEKWHSSMVPHAGPDSQIFRDVCELGADLNKLADE
                     GLLSTKLVKSKVAVVFDYESQWATEHTATPTQEVRHWTEPLAWFRALADNGLTADVVPV
                     RGPWDEYEAVVLPSLTILSEETTRRVREYVANGGKLFVTYYTGLVDDKDHVWLGGYPGS
                     IRDVVGVRVEEFAPMGNDFPGAMDHLDLDNGTVAHDFADVITSVADTAHVVASFKADKW
                     TGFDGAPAITINDFGDGKAAYVGARLGREGLAKSLPALLEELGIETSAEDDRGEVLRVE
                     RADETGENHFVFLFNRTHDVAIVDVDGEPLVASLAQVNESEHTAAIQPNGVLVVKL"
     gene            5715..7061
                     /locus_tag="Bbr_0530"
     CDS             5715..7061
                     /locus_tag="Bbr_0530"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|D6ZW64|3.A.1.1.48"
                     /protein_id="ABE95227.1"
                     /translation="MKFTTAKKAVALTGAVAMLVSVAACGSDSGKSSQPAQDSDVKEIT
                     VWAWEPSLTQVAKDFEKETGIKVDLKNVGTNTKEYTQLDNAIEAGSGAPDVAQVEYYAV
                     PQYAIKGNLLDITDKTSGYKDFYTPGPWASVQFAGKVYGLPMDSGPMAFFYNKEVFDKA
                     GVDAEQIKTWDQYYDAAKKIHALGDNYYITSDTGDAGFFDSMTWLAGAKPFQTSSDGSE
                     VTVNLTEDKGVKTFTDFWQKLLDEGLLDTKTAGWSEDWFKGMVDGTIASLFTGAWMPAN
                     LANSAADGAGKWRVTQMPTADGSTTNSENGGSSLAVLASTKKADAAYQFIEYANHGAGV
                     ATRVAGGAFPADKASLEKDSFKNATTVKNADGQDVDYFGGQKYNEVLAQAAENVSSGYQ
                     FLPFEVKARTIFGDYFGKSYTGDQKLSDGVAAWQKALQDYGKDQGFTVK"
ORIGIN
        1 tcagctaatg tcgcggacgg aaccgcgctt gatgagttcg gtggggatta cggttttgat
       61 gccggccggt actttctcgc cttcgcattg acggcggacc atggagaacg cggtggcgcc
      121 gatggcgtca ttgcgcatgc gcatggtggt gaggttgagt cttggcacca cgtcgaccag
      181 cgaatcgtcc acgcccacaa tgcttacgtc ttccggcacg cgcttgccgg ctgattccaa
      241 tccgagcatc gcaccgtagg ccatctgatc gttggccgca tagattgcgg tgcattccgg
      301 gtcgtgagcc agcgccacgc ctgcctgata gccggagtcg gcacaccagt cgccgatata
      361 catgctcggt acgcgcgcgc ctatttgttc caaggcttca cgccagcctt gcgctcggct
      421 ttcggcagca cgtgaagtga ctgggccggc gatatggtag acggtcttat ggcctttgct
      481 catcaaatag tcgacgatgg ctgtggagca tccgtattga tcggcgtcaa cggtggggca
      541 gtggtcggct gccgcctcgg aaatcagcac aaccggtagt tctcgcggtg gtttgtatcc
      601 cacgaagtcg gaaatgcgtt cctcaagcac cacgatgacg ccatctacgg gttgacgttg
      661 catgcggtcg atggcggagg ccagcgtacg ttctgcgccg ttgctcatag tctgaatggt
      721 gacggaatag ccgtcctcgg ctgcatgggt gacaatggaa tcgagtatcc gggcattacc
      781 gaacctggtg gtgtcgaaca tcactacgcc gatatcctgg aatcgcccat gcttcaatgc
      841 tcgggcggcg taattcggac gatatccgag gcgttccatc gcggcttcaa cgcgctggcg
      901 ggtttccggc ttgaccgcat tgctgtcatt cgccacacgg gatacggttt gcggcgaaac
      961 gccggccgcc ttagccacat cttgcatgga aacctgtgtt ggtctcgcca tctcgcccct
     1021 cgcttgtctc tcaaacgaca gcggtcatac cgctgcgatg tctaccatgt atcgcgtgaa
     1081 cggtaacgat aacatcattg ttttcgtgac cattgtaaca gctagcgcaa aaacattgaa
     1141 ttcaacacga ttggacaatg ttatgaatgg tgttatgatg ataacgtaaa caattttggt
     1201 caaccttcga aggagagggg ttggaaatgg cttcagccga tgtcgctgcc gtgcctatgc
     1261 acacggataa agcgcgtgag aacaagcata aggccgattg gcgtggatgg aagttcatgt
     1321 ggccgttcac ggtggtgttc gtgttcgtgt tcattattcc gattgtgtac gccgtttaca
     1381 tcagcttctt caagaagcag atgatcggcg gcacgaggtt cgtcggggtc gagaactacg
     1441 cgcgtctgct gcaggacggc cagttctggt cgtccgtggg tcgtgtggcg ctgttcacct
     1501 gcgtgcaggt gccgatcatg ctgttcctgt cggctgccat ggccctcgcc ctggattcga
     1561 tgaagctgca cggcgccaag ttcttccgca tcagcacgtt cctgccctat gcggtgcccg
     1621 cagtggtgtc cacgttggtg tggggcttcg tgtacggcgc gaagtacggc ctggtgggct
     1681 cgttcaacga cttcttcggc acgaacgtcg acgtgctgca gccgggcgtg ctgcttgcct
     1741 ccatcggcaa catcagcacg tgggagttca ccggctacaa catgctgatc ttctactcgt
     1801 cgctgtccac catcccgcac tccctgtacg aggccgcctc catcgacggc gcctccgaat
     1861 ggcagatcgt caagtccatc aagctgcccg agctgaaggg ctcgctggcc atcacggtga
     1921 tcttctcgat catcggcagc ttccagctgt tcaacgagcc gagcatcttg cagaacatgg
     1981 ttccgggcaa cgcgatcacc acgtactaca cgccgaacat gtacgcgtac aacctgagct
     2041 tctccggcaa ccagtcgaat tacgcggccg ctctggccat cgtgatggcc gtcatcacca
     2101 tggccatcgc ctacgccgtg cagctgaaga gcatgaagga gcagatgaag tgacgacgac
     2161 cgcccgtatt gccggattgg tttcgattgt gaaggagcgt gcgtgatgag tgccgcagca
     2221 tctacccgtc tgactgacgc cgaactgaag gcgcgggaga aggccgccag gaaggccgag
     2281 aaggaacgcc agaagcgcat cgccgccgat gaggcggccg agcgcaagcg cagcgccagg
     2341 gccggtttcg cgaacgtgaa caacccgcgc cgttcgaccc tgatgaccgt gctgtgcgcg
     2401 atcttcgccg tgtactgcct gttcccgttc gtgtacctgg tgatcaacgc caccaagacc
     2461 caggccgact tcacctccac gttcggcctc ggcttcggca agagcttcgc cctgtgggac
     2521 aacatcgtca cggtgttcac ctaccagggc ggcatcttcg gccgctggct ggtcaacacc
     2581 ctgctctacg tggtcgtcgg cgccggcggc gccaccctcc tggccatcat gggcggctac
     2641 gcgctggcca agttccgctt ccccggccgc aaggccgtgt tcgccgtgat catcggctcg
     2701 atctccgtgc cgggcatcgc cctggccgtg ccgcagttcc tcctgttcgc caagctgggc
     2761 ctgaccaaca ccccgtgggc catgatcatc ccgtcgctga tctccccgtt cggcctgtac
     2821 ctgatgtgga tcttcagcga gcaggccgtg cccaccgagc tcctggaggc cgcccgcgtg
     2881 gacggcgcgt ccgagttccg cacgttctgg accatcagcc tgcccctttt ggcccccggt
     2941 atcgtgacga ccgccctgtt cacgatcgtg gcgacgtgga acaactactt cctgccgttg
     3001 atcatgttga aggacgccga ctggtacccg ctgaccatcg gtctgaacca gtggaaggac
     3061 caggcctcga ccgcaggcgg ccaggccatc cagaacctgg tgatcaccgg ctccctgatc
     3121 acgatcatcc cgctggtcat cgcgttcctg tgcctgcaga agtactggca gtccggcctc
     3181 gccgcaggcg ccgtcaagga gtaaccgtac ttcggctccc ttctggtgga gggagccgag
     3241 ataaaggcat aaccactaac aggaaggagg cgtctatgca gatattcgca ttgttgagaa
     3301 tcgtgcaggc gcctccttgt gtatctgcgt gactcccatt tgcccacacg tagatattta
     3361 ttcattaaag gaacagcatc atcatggaac atcgcgaatt caagtggccg cagcctcttg
     3421 cgggtggcaa gccccgcatc tggtacggcg gcgattacaa ccccgaccaa tggcctgagg
     3481 aagtgtggga cgaagatgta gccctcatgc agaaggccgg cgtcaacctc gtctccgtag
     3541 ccatcttctc ttgggccaag cttgagcccg aagaaggcgt gtatgacttc gattggctcg
     3601 accgcgtcat cgacaagctc ggcaaggccg gcatcgccgt cgatctcgcc tccggcaccg
     3661 catccccgcc gatgtggatg acccaggccc acccggagat cctctgggtc gactaccgcg
     3721 gcgacgtctg ccagcccggt gcccgacagc actggcgggc caccagcccg gtcttccttg
     3781 actacgcgct caacctgtgt cgcaagatgg ccgagcacta caaggacaac ccctatgtgg
     3841 tctcttggca cgtgagcaac gagtacggct gccacaaccg cttcgactac tccgaagatg
     3901 ctgagcgcgc cttccagaag tggtgcgaga agaagtacgg caccatcgac gccgtcaatg
     3961 acgcctgggg caccgccttc tgggcgcagc gcatgaacaa cttctccgag atcatcccgc
     4021 cgcgcttcat cggcgatggc aacttcatga acccgggcaa actgcttgac tggaagcgct
     4081 tcagctccga cgccctgctc gacttttaca aggccgagcg cgacgccctg ctcgagatcg
     4141 cccccaagcc gcagaccacc aacttcatgg tctccgccgg cggtgccggt atcgattacg
     4201 acaagtgggg ttacgacgtg gacttcgtgt ccaacgatca ctacttcact cctggcgaag
     4261 ctcacttcga tgagctggcc tactcggcct ccctgtgtga cggcatcgcc cgcaagaatc
     4321 cgtggttcct catggagcac tccagctccg ccgtcaactg gcgtccgatc aactaccgcg
     4381 tcgaacccgg cgaactggtg cgcgactccc tggcccacct ggccatgggc gccgacgcca
     4441 tctgctactt ccagtggcgc cagtccaagg ccggtgccga gaagtggcac tcctcgatgg
     4501 ttcctcacgc gggccccgac tcccagatct tccgcgacgt gtgcgagctg ggtgccgacc
     4561 tcaacaagct tgctgacgag ggcctgctga gcaccaagct ggtcaagtcc aaggtcgccg
     4621 tcgtcttcga ttacgagtct cagtgggcta cggaacacac cgccacgcct actcaggagg
     4681 tacgccattg gaccgagccg ctcgcgtggt tccgcgcgct ggcggacaac ggtctgaccg
     4741 cagacgtggt gccggttcgc ggtccctggg acgagtacga agccgtcgtg ctgccgagcc
     4801 tgaccatcct gtctgaagag accacgcgcc gcgtgcgcga gtatgtggcg aacggcggca
     4861 agctgttcgt gacctactac accggtctgg tggacgacaa ggatcatgtc tggctgggtg
     4921 gctaccccgg ctccatccgt gacgtggtgg gcgtgcgcgt tgaggaattc gccccgatgg
     4981 gcaacgactt ccccggtgcc atggaccacc tcgacttgga caacgggacc gtggcgcacg
     5041 atttcgccga cgtgatcacc tccgtggctg ataccgctca cgtggttgct tccttcaagg
     5101 ctgataagtg gaccggtttc gacggcgctc ccgccatcac catcaacgac ttcggcgacg
     5161 gcaaggccgc atacgtcggt gcccgtctcg gccgtgaggg cttggccaag agcctgcccg
     5221 cgctgctgga ggaactcggc atcgagacct cggccgaaga cgatcgcggc gaagtgctgc
     5281 gcgttgagcg tgcggatgaa actggcgaga accacttcgt gttcctgttc aaccgcaccc
     5341 acgatgtggc gattgtggac gtggacggcg agccgctggt cgcctcgctg gctcaggtca
     5401 acgaatccga gcatacggcg gccattcagc cgaacggtgt gctggtggta aagctgtaaa
     5461 gaggtctatt tatagcgatt caaatgcgat ggcgggcgtg ttagcgataa cacgcccgcc
     5521 atcgcatttg cgatttgttt tgagatgtgt atactaaaag atgttatcgc aaccatagcg
     5581 aaaggaatgg cttcatagag ccacttctca gtgtcgagga gaaatcaata attgacacta
     5641 ggtaacacgc aatgattgcg agcgacaaaa cagtttgaat cgttctagac gattcgagga
     5701 aggagaacag aacgatgaag ttcactaccg ccaagaaggc cgttgccctc accggtgcgg
     5761 ttgccatgct ggtttccgtt gccgcctgcg gctctgatag cggcaagtcc agccagccgg
     5821 ctcaggattc cgacgtcaag gaaatcaccg tctgggcttg ggagccttcg ctgacccagg
     5881 ttgccaagga cttcgagaag gagaccggca tcaaggtcga cctgaagaac gtcggtacca
     5941 acaccaagga atacactcag ctggacaacg ccattgaggc tggctctggc gctccggacg
     6001 tcgctcaggt tgagtactac gccgtcccgc agtacgccat caagggcaac ctgctggaca
     6061 tcaccgacaa gacctctggt tacaaggact tctacacccc tggcccgtgg gcttctgtgc
     6121 agttcgccgg caaggtctac ggcctgccga tggattccgg cccaatggcc ttcttctaca
     6181 acaaggaagt cttcgacaag gccggcgttg atgccgagca gatcaagacc tgggatcagt
     6241 actacgacgc cgccaagaag attcacgccc tcggcgacaa ctactacatc acctccgaca
     6301 ccggcgacgc cggcttcttc gactccatga cctggctggc cggcgccaag ccgttccaga
     6361 cctcctccga tggttccgaa gtcaccgtca acctgaccga agacaagggc gtcaagacct
     6421 tcaccgactt ctggcagaag ctgctggacg agggtctgct cgacaccaag accgccggct
     6481 ggtccgagga ttggttcaag ggcatggtcg acggcaccat cgcctccctg ttcactggcg
     6541 cttggatgcc tgccaacctt gctaactccg ctgctgacgg tgccggcaag tggcgtgtga
     6601 cccagatgcc gaccgctgac ggctccacca ccaactctga gaacggtggt tcttcgctgg
     6661 ctgtgctcgc ctccaccaag aaggctgatg ctgcttacca gttcatcgag tacgccaacc
     6721 acggtgctgg tgtggccact cgtgtggctg gcggcgcctt cccggctgac aaggcctccc
     6781 tggaaaagga ctccttcaag aacgccacca ccgtgaagaa cgccgatggt caggatgttg
     6841 actacttcgg tggtcagaag tacaacgagg ttctggctca ggctgctgag aacgtgtcct
     6901 ccggctacca gttcctgccc ttcgaggtca aggcccgcac catcttcggc gactactttg
     6961 gcaagtccta caccggtgac cagaagctga gcgacggtgt cgctgcttgg cagaaggccc
     7021 tgcaggatta cggcaaggat cagggcttca ccgtgaagta a
//
