LOCUS       CP000303                6197 bp    DNA     linear   BCT 31-JAN-2014
DEFINITION  Bifidobacterium breve UCC2003, complete genome.
ACCESSION   CP000303
VERSION     CP000303.1
DBLINK      BioProject: PRJNA13487
            BioSample: SAMN02604112
KEYWORDS    .
SOURCE      Bifidobacterium breve UCC2003
  ORGANISM  Bifidobacterium breve UCC2003
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   O'Connell Motherway,M., Zomer,A., Leahy,S.C., Reunanen,J.,
            Bottacini,F., Claesson,M.J., O'Brien,F., Flynn,K., Casey,P.G.,
            Moreno Munoz,J.A., Kearney,B., Houston,A.M., O'Mahony,C.,
            Higgins,D.G., Shanahan,F., Palva,A., de Vos,W.M., Fitzgerald,G.F.,
            Ventura,M., O'Toole,P.W. and van Sinderen,D.
  TITLE     Functional genome analysis of Bifidobacterium breve UCC2003 reveals
            type IVb tight adherence (Tad) pili as an essential and conserved
            host-colonization factor
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2011) In press
   PUBMED   21690406
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   O'Connell Motherway,M., Zomer,A., Leahy,S., Reunanen,J.,
            Bottacini,F., Claesson,M., O'Brien,F., O'Flynn,K., Casey,P.,
            Moreno-Munoz,J., Higgins,D.G., Shanahan,F., de Vos,W.,
            Fitzgerald,G.F., Ventura,M., O'Toole,P. and van Sinderen,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2006) Department of Microbiology, Alimentary
            Pharmabiotic Centre, University College Cork, College Road, Cork,
            Munster, Ireland
COMMENT     Source DNA or strain is available from Douwe van Sinderen,
            d.vansinderen@ucc.ie.
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Bifidobacterium breve UCC2003"
                     /mol_type="genomic DNA"
                     /strain="UCC2003"
                     /db_xref="taxon:326426"
     gene            complement(1..1482)
                     /gene="lacS"
                     /locus_tag="Bbr_1551"
     CDS             complement(1..1482)
                     /gene="lacS"
                     /locus_tag="Bbr_1551"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9X761|2.A.2.2.3"
                     /protein_id="ABE96222.1"
                     /translation="MSGSTTQRMATSEQPHATPSGNMGQKIAYAFGNLGQAAFYNTMST
                     FFITFVTTALFIDVDKTLAKRLIAVITGLIVVIRIAEIFLDPLLGNLVDNTNTRWGRFR
                     PWQFIGGIVPGILLIMVFTGLFGLVDVNTGVFMVLFVIVFILLDVIYSLRDISYWGMIP
                     AISSDSHERSTYTALGAFTGSIGYNGVTVIVVPVVSWFTWKFTGQWEQGRTGWAAFAII
                     IAVLGLLTAWSVAFGTRENQGELRAKAEANGNPIEAFKAIAQNDQLLWVALSYLLYSVA
                     NVATTGVLFYQFKYVLGAPDSFSIAGVIPVITGLVTTPLYPVLNRRIPRRWLYTAGMAL
                     MIAGYTLFIIAPMNLPVVIVALVLFYLPAQTIQMTAILSMTDSIEYGQLKTGKRNEAVT
                     LSVRPMLDKIAGALSNGIVGFVVVAAGMVGNATAADMTAANIRTFKTCAYYLPLAGIVA
                     SLVVFLLAVKIDENMHDHIVEQLEERFASEETDKQ"
     gene            1850..4987
                     /gene="lacZ6"
                     /locus_tag="Bbr_1552"
     CDS             1850..4987
                     /gene="lacZ6"
                     /locus_tag="Bbr_1552"
                     /EC_number="3.2.1.23"
                     /codon_start=1
                     /transl_table=11
                     /product="GH2"
                     /protein_id="ABE96223.1"
                     /translation="MNTTDDQLKNGDPIVSPSMPTTAWLTDPRVYAVHRLDAHSDHACW
                     SHAPVNGEGSDLGQSLDGEWRVRVETAPTGRFPDGTSDVSPLFAASDFDDSSFSRVQVP
                     SHLETAGLLAPQYVNVQYPWDGHEDPEAPAIPEHGHVAVYRREFDADGEVAQAVREGRP
                     VTLTFQGAATAIYVWLNGSFVGYAEDSFTPSEFDVTDAIKMEGNVLAVACYEYSSASWL
                     EDQDFWRLHGLFRSVELNARPSAHVADIHADTDWDPATSRGSLSLDILVDGTPNAATAD
                     LVLRDKNGTTVWHTSTEATGTLHAEAEIDGASPWSAERPDLYTLSVALLDADGRILEIA
                     RTRIGFRRVSIEDGILKLNGKRLVFRGVNRHEFDCRRGRAITEEDMLWDIRFMKRHNIN
                     AVRTSHYPNQSRWYELCDEYGIYLIDETNLETHGSWNSPGDIPVGTSVPGDDEAWLGAC
                     IDRLDSMIMRDRNHPSVLIWSLGNESYAGEVLKAMSAHAHRLDPGRPVHYEGVNWNHAY
                     DEISDFESRMYAKPAEIRDWLEHGDERGEASKPFVSCEYMHAMGNSCGGLSEFIDLERY
                     ERYSGGFIWDYIDQGLVQRLPDGCERLSVGGDWGDRPTDYEFVGNGIVFADRTPSPKAQ
                     EVKQLYSPVKLTPDGHGVTIENRNLFANTDGYVFAARLLEDGREIWHADYRFDVAAGDT
                     QHHDIAFPDIDSDEGTREVTYEVDLLLAEATAWAPAGYELAFGQLTDTLNPEGDITGND
                     QDDGRATVTLSRWNAGIRRDDEEILMSRTQGGIVSWKRNGREMVIRRPEIVTFRPLTDN
                     DRGNRSGYDRAAWFAAGRYAVVTDTSITQSDDGGLTAAYRYELADPDHTPVSVTYRVTP
                     GMRMQLTVEYPGNATGAASLPAFGIEWELPGEYQHLRYYGTGPEETYRDRKQGGKLGIW
                     DTTSEASTAPYLMVQETGSHEDVRWLEATDIQGHGLRIIQRGDRHFTASLLPWNTYTIE
                     AARRHEDLPAPRHNYLRLLAAQMGVGGDDSWGAPVHTAYQLPADRPLTLDVNLELI"
     gene            complement(5187..6197)
                     /locus_tag="Bbr_1553"
     CDS             complement(5187..6197)
                     /locus_tag="Bbr_1553"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955"
                     /protein_id="ABE96224.1"
                     /translation="MATLKEVAERAGVSQSTVSRLLNDSSFSIKEETRRRVLRVCEELG
                     YRNVFRPAIAVLDAPPSGEELQDVYFADLRKVLAECAESMELDPPASIRSIHELTERAS
                     EFDGFITVGATVFPEADLRALHAVLPHGVCIDTNPAPHLFDSVRPDLSQTMLDALDAMI
                     ATGRKRVAFLGGVGSIMGTHDYPEDIRELSFRQWATHLRLETDGLVYSSGTFTVENGYR
                     LAEQLVSDHRGTGTMPDGLIVAADVLAVGALQALNALRVAVPDELAVVSVNNQSIARYT
                     SPSLSSYAIDQRELARMAFSTLIDGLKHERRVRRHILLTTELVPRASFVPSAMMN"
ORIGIN
        1 ttactgcttg tcggtttcct cggaggcgaa tcgttcttcg agctgttcga cgatgtggtc
       61 gtgcatgttc tcgtcgatct taacggccag gaggaagacg acgaggctgg cgacgatacc
      121 ggcgagcggc aggtagtagg cgcaggtctt gaacgtgcga atgttcgcgg cggtcatgtc
      181 ggcggcggtg gcgttgccga ccattccggc ggcgacgacc acgaagccca cgatgccgtt
      241 ggacagggcg ccagcgatct tgtcgagcat cgggcgcacg gagagggtga cggcctcgtt
      301 gcgcttgccg gtcttgagct ggccgtattc gatggagtcg gtcatgctca ggatggcggt
      361 catctggatg gtctgcgcgg gaaggtagaa gaggacgagc gcgacgatga cgacgggcag
      421 gttcatgggc gcgatgatga acagcgtgta gccggcgatc atgagcgcca tgccggcggt
      481 gtacagccag cggcgcggaa tgcggcggtt gagcaccggg tagaggggcg tggtgaccag
      541 tccggtgatg accgggatga cgccggcgat ggagaagctg tccggggcgc cgagcacgta
      601 tttgaactgg tagaagagca cgccggtggt ggcgacgttg gccacggagt acagcaagta
      661 gctgagggcg acccacagga gctggtcgtt ctgggcgatg gccttgaacg cctcgatggg
      721 gttgccgttg gcctcggcct tggcgcgcag ctcgccctgg ttctctcggg tgccgaacgc
      781 gacgctccac gcggtgagca agccgagcac ggcaatgatg atggcgaacg ccgcccagcc
      841 ggtgcggccc tgctcccact ggccggtgaa cttccaggtg aaccagctga cgacgggcac
      901 gacgatgacg gtgacgccgt tgtagccgat ggatccggtg aacgcgccga gcgcggtgta
      961 cgtgctgcgc tcgtgcgagt cggaggaaat cgcggggatc atgccccagt acgagatgtc
     1021 gcgcagcgag tagatcacgt cgagcaggat gaacacgatg acgaacagga ccatgaacac
     1081 gccggtgttc acgtccacga ggccgaacag gccggtgaac accatgatca gcaggatgcc
     1141 gggcacgatg ccgccgatga actgccaggg acggaaccgg ccccatcggg tgttcgtgtt
     1201 gtccacgagg ttgccgagca gcgggtcgag gaagatctcc gcgatgcgga tgaccactat
     1261 gagtccggtg atcaccgcga tgaggcgctt ggccagcgtc ttgtccacat cgatgaacag
     1321 cgcggtggtc acgaatgtga tgaagaacgt gctcatcgtg ttgtagaacg cggcctggcc
     1381 caggttgccg aatgcgtatg cgatcttctg ccccatgttt cctgaaggcg tggcatgcgg
     1441 ctgttccgac gtcgccatgc gctgtgtggt ggatccgctc atggtgtggt ggcctccttg
     1501 cgacctataa agaatccgtg cgcgggaacc gctccaatcc cgcaaagcgt cagtatagaa
     1561 ctctcttgaa aaagtaaaaa aatttaccgc gtgtcgcaaa tcatgccaac gttttgcaac
     1621 cagcactccg tgtggatgag taaggtttga agcttgcttg atgtgcttgg gtcttaagaa
     1681 atccacgtat tttgcatgtt gtaggcctta tgccgcgaga tgctggaaag aatttgcgca
     1741 atcaagtaaa aatatttatc cttattatat ataaggaacc cgattcaacg aggttccctc
     1801 actgcggcgg caacgacgcg acgcaatccg atgcgaaagc gaggacatca tgaacacaac
     1861 cgacgatcag ctgaagaacg gcgatccgat cgtctcaccg tccatgccga caacggcatg
     1921 gctcacggac ccgcgcgtgt acgcggtcca ccggctcgac gcccattccg atcatgcgtg
     1981 ctggtcgcac gcccccgtca acggcgaggg ttctgatctc gggcagagcc ttgacggcga
     2041 atggcgggtc cgcgtcgaga cggcgccgac gggccgtttc cccgatggga cgagcgacgt
     2101 gtcgcctctg ttcgccgcgt ccgacttcga cgattcgtcg ttctcccgcg tgcaggtgcc
     2161 ctcgcatctg gagactgcgg ggctgcttgc cccgcagtac gtgaacgtgc agtacccatg
     2221 ggacggacat gaggacccgg aggctccggc catccccgag cacggccatg tggcggtcta
     2281 ccggcgcgag tttgacgcgg atggcgaagt cgcccaggcc gtgcgcgagg ggcgcccggt
     2341 gacgcttacc ttccagggcg cagccacagc catctacgtg tggctcaacg gttcgttcgt
     2401 tggctacgcc gaggactcct tcacgcccag cgagttcgac gtgacggacg cgatcaagat
     2461 ggaaggcaac gtgctggcgg tcgcctgcta cgagtattcg agcgcgagct ggttggagga
     2521 tcaggatttc tggcggctgc acggcctgtt ccgctccgtc gaactcaacg cgaggccttc
     2581 cgcccatgtc gccgacatcc acgccgacac cgattgggac cccgccacat ctcgcggatc
     2641 gctctcgctg gacatactag tcgacggcac accgaacgcc gcgacggccg acctcgtgct
     2701 gcgcgataag aacggcacga ccgtctggca cacctccacc gaagcgacag gaacactgca
     2761 cgccgaggcc gagatcgacg gcgcgtcgcc atggagcgcg gagcgacccg acctgtacac
     2821 cctgtccgtc gccctgctcg acgcggacgg caggatcctg gaaatcgcgc gcacccgcat
     2881 cggcttccgc cgcgtatcca tcgaggacgg catcctcaag ctcaacggca agcgcctcgt
     2941 gttccgcggc gtcaaccgcc acgagttcga ctgccggcgc ggccgggcca tcaccgaaga
     3001 ggacatgctg tgggacatcc gcttcatgaa gcgccacaac atcaacgcgg tacgcacctc
     3061 gcactatccg aaccagtcac gctggtacga actgtgcgac gaatacggca tctacctgat
     3121 cgacgagacc aacctggaaa cccacggcag ctggaacagc cccggcgaca tccccgtggg
     3181 aacctccgtc cccggcgacg acgaagcctg gctcggcgca tgcatcgacc gactcgacag
     3241 catgatcatg cgcgaccgca accatcccag cgtgctcatc tggtcgctgg gcaacgaatc
     3301 ctatgcgggc gaagtcctca aggccatgag cgcgcacgcg caccggcttg atccgggtcg
     3361 tcccgtccac tacgaaggcg tcaactggaa ccacgcctac gacgagatca gcgatttcga
     3421 gagccgtatg tacgccaagc cggccgagat ccgagactgg ctcgaacacg gcgacgaacg
     3481 aggcgaggcg agcaagccgt tcgtcagctg cgagtacatg catgccatgg gcaactcatg
     3541 cggcggcctg agcgagttca tcgacctcga acggtacgag cgctactccg gcggattcat
     3601 ctgggactac atcgaccaag ggctcgtcca gcgtctgccc gacggatgcg aacgcctcag
     3661 cgtcggcgga gactggggcg accggccgac cgactacgaa ttcgtgggca acggcatcgt
     3721 attcgccgac cgcaccccca gccccaaggc gcaggaggtc aagcagctgt attcgccggt
     3781 caagctcacc cccgacggac acggcgtgac catcgagaac cgcaacctgt tcgccaacac
     3841 cgacggctat gtattcgccg cacggctcct cgaagacgga cgcgagattt ggcatgccga
     3901 ctaccgtttc gacgtggccg caggagatac ccaacaccat gacatcgcct tcccggacat
     3961 cgactccgac gagggcacgc gcgaggtcac ctatgaggtc gatctcctgc tcgcagaggc
     4021 caccgcgtgg gcacccgccg gctacgaact cgccttcgga caactcaccg acaccctcaa
     4081 ccccgaaggg gacatcaccg ggaacgacca agacgacggc cgcgcgacgg tcactctcag
     4141 ccgatggaac gccggcatcc gccgcgacga cgaggaaatc ctcatgtcac gcacccaggg
     4201 aggcatcgtc tcctggaagc gcaacgggcg ggaaatggtc atccgccgtc ccgagatcgt
     4261 caccttccgt ccgctgaccg acaacgaccg cggcaatcgc tccggatacg accgggccgc
     4321 atggttcgcg gccggccgct acgccgtcgt gaccgatacg agcatcacgc aatccgacga
     4381 tggcggactc acggccgcat accggtacga actcgccgac ccggatcaca cgccggtgtc
     4441 tgtcacttac cgcgtcacgc ccggcatgcg catgcaactg accgtcgaat accccggcaa
     4501 cgccacagga gcggccagcc tgcccgcatt cggcatcgaa tgggagctgc caggcgaata
     4561 ccagcacctg cgctactacg gtaccggccc cgaggagacc tatcgcgacc gcaagcaggg
     4621 cggcaagctc ggcatctggg acaccacctc agaggcgagc acggcaccgt atctcatggt
     4681 gcaggaaacc ggcagccatg aggacgtccg ctggctcgaa gccaccgaca tccaaggcca
     4741 cggattgcgt atcatccaac gcggcgaccg ccacttcacg gcgagcctgc tgccctggaa
     4801 cacctacacg atcgaagccg cgcggcgcca cgaggacctg cccgcgccgc gccacaacta
     4861 tctgcgcctg ctcgcggccc agatgggagt cggcggagac gactcctggg gagcccccgt
     4921 ccacacggcc taccagctgc ccgccgacag gccgctcacc ctcgacgtga acctcgaact
     4981 catctgaccg gcaacgggcg gcggcatgca ccaccatgcc gccgcccgta cccgtatata
     5041 ttcatattcc ccaagcacaa caacgacatc aaccaggtgc cgacccactg catcacgcag
     5101 ggcatcggca cggtgctcaa ggcccgccac ctcgtgctgc tcgcgttcgg cgcgggcaag
     5161 gccgaggcca gtgccgtccg tcttcgtcaa ttcatcatgg ccgaggggac gaaactggct
     5221 cgcggcacca gctcggtcgt cagcagaatg tgccgtcgca cccgacgctc atgcttcaac
     5281 ccatcgatca gggtggagaa tgccatgcgc gccaactccc tttgatcgat tgcatatgag
     5341 ctcagcgacg gtgacgtgta ccgagcgatc gactggttgt tcacgcttac cacggccaac
     5401 tcgtcaggaa ccgccacgcg tagcgcgttc agtgcttgca atgcccccac cgcgagcacg
     5461 tcagccgcca cgatcagacc gtcgggcatg gtgcctgtcc cgcgatggtc agaaaccaat
     5521 tgctccgcga gacgatagcc gttctccacg gtgaacgtgc cggacgaata caccagcccg
     5581 tccgtttcca atctcaagtg agtagcccac tgacggaacg acagctcgcg gatatcctcc
     5641 ggataatcat gcgtacccat gatgctgcct acgccgccaa gaaacgcgac acgcttgcgg
     5701 cccgtcgcga tcattgcatc cagagcgtcc agcatcgtct gcgacagatc gggtcgcacc
     5761 gaatcgaaca gatgaggcgc cggattcgtg tcgatacaca cgccatgagg cagcaccgcg
     5821 tgcaacgcac gtaaatccgc ctcggggaac accgtggcac ccacggtgat gaacccgtcg
     5881 aactccgatg cgcgctccgt cagctcatgt atggaacgga tggatgcggg agggtccaat
     5941 tccatcgatt ccgcgcactc cgcaaggact ttccgcagat cggcgaagta tacatcctgt
     6001 agctcctccc cagacggagg cgcatccagc actgcgatcg cagggcggaa cacgttgcga
     6061 taccccagct cttcgcatac acgcagcaca cgccgccgcg tctcctcctt gatggagaac
     6121 gatgagtcgt tcagcagacg cgacaccgtg ctctgcgaca ccccggcccg ttccgcgact
     6181 tccttgagcg tggccat
//
