LOCUS       JF327803               13109 bp    DNA     linear   BCT 24-SEP-2012
DEFINITION  Geobacillus stearothermophilus strain T-6 methyl-accepting
            chemotaxis protein (mcp) gene, complete cds; and galactan
            utilization gene cluster, complete sequence.
ACCESSION   JF327803
VERSION     JF327803.1
KEYWORDS    .
SOURCE      Geobacillus stearothermophilus
  ORGANISM  Geobacillus stearothermophilus
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Tabachnikov,O., Shulami,S. and Shoham,Y.
  TITLE     The galactan utilization gene cluster in Geobacillus
            stearothermophilus T-6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13109)
  AUTHORS   Tabachnikov,O., Shulami,S. and Shoham,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2011) Department of Biotechnology and Food
            Engineering, Technion-Israel Institute of Technology, Haifa 32000,
            Israel
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Geobacillus stearothermophilus"
                     /mol_type="genomic DNA"
                     /strain="T-6"
                     /culture_collection="NCIMB:40222"
                     /db_xref="taxon:1422"
                     /note="type strain of Geobacillus stearothermophilus"
     gene            complement(81..1775)
                     /gene="mcp"
     CDS             complement(81..1775)
                     /gene="mcp"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|M3JA40|1.B.52.2.1"
                     /protein_id="AEH26457.1"
                     /translation="MNVMFSCTIVLLTTVIMTVASIQIKEGIKKETLERVKSNLILAYR
                     YIDEKYKGNWMLKDGMIYKGETQINGDNQLVDEIGKMIGGTVTIFQNDTRVATNIILHG
                     QRAIGTTAPKPVVNKVLKEGGTFFREVEILGEWYQAAYMPIKSHNGEIIGMFYVGIPQK
                     HVNTVVSSLFRMLLLSAVAVLAIAASVTVWFTRHIRVRLDAVSKALQQAGEGDFTVTLN
                     DGARDEIGQIVESYNKMRDNLSELLARVTAASEQVAAAAEELTASAEQTVLSTEQVSAG
                     IQEVANRADIQSSKTDATAASMEQMAKGVASIVDRSTAIADLSVHMIAQAEDGGKSVEK
                     TVEQMNAIQQSVLQSEKVIRSLQERSKEIESIVNIINQIAEQTNLLALNAAIEAARAGE
                     HGAGFAVVADEVRKLAEQSQESTKQIAHLIARIQQDIENTAQIMGDVIQNVQSGMNTSQ
                     ETSQKFHVILKNTKTISAQLEDVSASVQQLSAGIQELDSTITELAATAEKTMETSKEMA
                     ASAEEQVASMEEITSSAQSLANLSETLQEIAQTFHIVPKEASRFKQS"
     misc_feature    2379..11724
                     /note="galactan utilization gene cluster"
     gene            2379..3371
                     /gene="ganR"
     CDS             2379..3371
                     /gene="ganR"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|LacI,DBD-SUPERFAMILY|0044558"
                     /protein_id="AEH26451.1"
                     /translation="MATIRDIAKQAGYSISTVSRVLNNDPDLSVSEETREKIYEVAEKL
                     NYRKKTIRKVIRDIAFLYWLTDKEELEDVYYKTMRMEMEKLAKEFNLELQMYKISDGID
                     QIPENIEGFIAVGIFSDRELERLRSITTNGVFIDLSPDPRCFDSVRPDLTQITERAIDF
                     FLEKGHTDIGFIGGTYPNPNTGKDEMDSRERAFRNYMKAKGLLKEEYIFCHRGFSFDNG
                     YELMTKAIETLGDRLPTAFFVAADPIAVGCLQALNEHRIAIPDRVSVISVNNISITKYV
                     SPPLTTFHIDIHEICKSAIELLLERIQRKRKIVKTLLLGAELIVRKSTN"
     gene            3493..4782
                     /gene="ganE"
     CDS             3493..4782
                     /gene="ganE"
                     /note="sugar binding protein"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|O07009|3.A.1.1.2"
                     /protein_id="AEH26452.2"
                     /translation="MMRSISKHLALVSVMAFSLSLAACGPKENANQGSRAGENKENNGK
                     VELVVWEDIQKSAGIKDAIAQFEKENNVKVKVIEKPYAKQIEDLRLDGPAGTGPDVLTM
                     PSDQIGTAVTEGLIKELNVGEDIQSIYTEAAMQSQKVNNKVYGLPKAVETTTLYYNKDL
                     ISEEELPKTLEEWYEYSKKVTDGKKFGFLALFDQIYYAQSVMSGYGGYIFGKDENGNYN
                     PKDIGLNNAGAVEGAKIIQKFYKEGLFPSGMIGEQGINVLESLFTEGKAVAIISGPWNI
                     EPFTKAGINFGVTKLPALENGKNMSAFIGVKSYNVSAYSKHPDLAEKLVIFLANEENSK
                     KRYEVTKEVPAVKALVNDPIVKNSEAARAVAEQSQFSEMIPNIPEMNEVWKPADSALQT
                     IATGKAEPKVALDQAVKTIKGQIEAKHSGK"
     gene            4957..6213
                     /gene="ganF"
     CDS             4957..6213
                     /gene="ganF"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|O32261|3.A.1.1.2"
                     /protein_id="AEH26453.2"
                     /translation="MPHRKIALFLSIIPGLGQLYNRQWAKGILFLVFSVSFLIVFADLF
                     NMGFWGIVTLGTEVQRDNSIFLLAEGIIAVIVAVFGLTFYYFNLRDAYKNGERRDRGIR
                     LSTLKEDYHQLIEQGYPYVISSPAFFLLIFSVIFPVLFSFALAFTNYDLYHSPPARLVD
                     WVGFDTFLKIFTVDIWRSTFFDVLAWTVVWTVVASTLQVAIGILLAVVVNQKDLRLKNF
                     YRTIFVLPWAVPGFVTILVFAGLFNDSFGAINHTILAALGIDPIPWMTDEHWTRVALIL
                     MQGWLGFPYVFVVTTGVLQSIPEELYEAATIDGASVFAKFRYITLPMVLLAMAPIIITQ
                     FTFNFNNFNIIYLFNGGGPAVPGSTAGGTDILVSWIYKLTMQSSQYALAAALTILLSLF
                     VMGIALWQFRRTNSFKEGV"
     gene            6217..7068
                     /gene="ganG"
     CDS             6217..7068
                     /gene="ganG"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|O07011|3.A.1.1.2"
                     /protein_id="AEH26454.1"
                     /translation="MRASVRSKKFLRLFVSYAILTFVAMAIIYPLLWTIGASLNPGNSL
                     ISTTLIPNNPTFDHYRELFAGKESLPYARWYWNSMKISFFTMIGSVISVSFTAYAFSRF
                     RFKGRKNALTLFLLLQMVPQFSALIALFVLAQMLGMINSHWLLILLYIGGQIPMNTYLM
                     KGYMDSIPRDLDESAKIDGASSTRIFFQILLPLSKPMIAVVAMNGFTGPLGDFVLSSAI
                     LRTPDAYTLPIGLYNLVNEVMGASYTTFAAGALLISIPVAIVFLMLQRNFVSGLTLGGI
                     KG"
     gene            7088..9151
                     /gene="ganB"
     CDS             7091..9151
                     /gene="ganB"
                     /EC_number="3.2.1.23"
                     /function="hydrolyzes beta-1,4-D-galactosidic linkage of
                     galactobiose and short galacto-oligosaccharides"
                     /note="glycoside hydrolase family 42"
                     /codon_start=1
                     /transl_table=11
                     /product="GH42"
                     /protein_id="AEH26455.1"
                     /translation="MPKYERTYTTQANFILHGGDYNPDQWLDRPDILQADLELMKLSHT
                     NTFTVGVFAWSALEPEEGVYRFEWLDKVFDDIYRIGGRVILATPSGARPAWLSQKYPEV
                     LRVNAARVRQLHGGRHNHCFTSSVYREKTQHINRLLAERYGDHPALLMWHVSNEYGGEC
                     HCNLCQEAFREWLKKKYNHDLDALNAAWWTSFWSHTYTDWSQIESPSPIGEHTIHGLNL
                     DWKRFVTDQTISFFENEIVPLRELTPHIPITTNFMADTHDLIPFQGLDYSKFAKHLDVI
                     SWDAYPAWHNDWESTADLAMKVGFINDLYRSLKQQPFLLMECTPSLVNWHKVNKAKRPG
                     MHFLSSMQMIAHGSDSILYFQWRKSRGSFEKFHGAVVDHDNRTDSRVFQEVAEVGKALK
                     KMSGIVGTNRPAEVAILYDWENNWALNDAQGFAAETKRYPQTLVQHYRPFWERDIPVDV
                     ITKEHDFSRYKLLIAPMLYLVSEETIARLKEFVANGGTLVMTYISGIVDEHDLAYLGGW
                     HQDLREMFGMEPIETDTLYPRDRNSVHYRGRSYELKDYATVIKIHAATVEGVYEDDFYA
                     DTPAVTSNQYGKGQAYYIGGRLEDQFHRDFYQELMEKLDLRPVLFVKHEKGVSVQARQA
                     PECDYVFIMNFTEEKQAVVLEEKVKDLFTGEEIVGEIMLDKYEVRVVEKRR"
     gene            9280..11724
                     /gene="ganA"
     CDS             9280..11724
                     /gene="ganA"
                     /EC_number="3.2.1.89"
                     /function="hydrolyzes beta-1,4-galactan yielding
                     galactotetraose"
                     /note="glycoside hydrolase family 53"
                     /codon_start=1
                     /transl_table=11
                     /product="extra-cellular endo-beta-1,4-galactanase
                     precursor"
                     /protein_id="AEH26456.2"
                     /translation="MGKQFLFVLVAMMVMIMSFSSNHIHKAAADSYNYLINGGFESDFW
                     ADGSWTVTTQDWSSVDLQRFSYSNDAWIVPNEGDSAFKYWIRDIANGNQTFTLKQTIGQ
                     LPPGSYELSVQSMGGAGDEAGYIQLFAGDKMADAVVTKGYNSWETVKLEFTLEEEASNF
                     EVGAIVTGEPNAWGYMDHFQLVSLNSAGGREMPKPVPADIFVKRVDGLSKEFIKGVDVS
                     SIIALEDSGVKFYNAAGKKQDIFKTLKEAGINYVRVRVWNDPYDAKGHGYGGGNNDLEK
                     AIEIGKRATASGMKLLVDFHYSDFWADPAKQRPPKAWAKLNFEAKKKALYRFTKESLKA
                     MLKEKIQVGMVQIGNETNGAFVGETDWAKICELLNAGSRAVRETSPNILVVLHFTNPET
                     PGRYASIAKTLAEHKVDYDVFASSYYPFWHGTLANLTSVLKYVANTYLKKVMVAETSYP
                     YTTEDGDGHENTAPKSSGQTLNYPITVQGQANAIRDVIQAVAAVGKPGIGVFYWEPAWI
                     PVGPPEQLKQNEKKWEKYGSGWASSFAAEYDPDAAMWHGGSAVDNQALFDFNGRPLPSL
                     NVFKYVDTGAVAPLKIDEIKDIYIRAAFGEQVVLPTTVTATYNDGSKHLVSVKWDQAAL
                     EQALRTGVGTYVIQGVVEGGETVKAHLEITPKNYVVNPSFEDQDRTMWNIIYRNGTSPH
                     TDYLEKATDAKSGNYSLHFYSNEAVDFQVEQTITGLKPGYYNLSMFLQAGDAPNAEIYL
                     YAKTGDKEYRAAASVNGWLNWSSPEIHDIYIADGTVTIGASIKANGGAWGTLDDFQLYQ
                     TK"
     sig_peptide     9280..9366
                     /gene="ganA"
     mat_peptide     9367..11721
                     /gene="ganA"
                     /product="extra-cellular endo-beta-1,4-galactanase"
ORIGIN
        1 tacgctcacg cgtggaagtg ggggtcttct cggcgcgaga tgataaaacg gttattgact
       61 ggagcgtgaa cgattcccgc ttagctttgc ttgaagcggg aggcttcttt cggaacgata
      121 tgaaatgttt gcgcgatttc ttgcaatgtt tccgacaaat tcgctaatga ttgggctgat
      181 gatgtaatct cttccattga agcgacctgt tcttcagcgg aagcagccat ttctttagac
      241 gtttccatcg ttttttcggc tgtggctgct agttcagtaa tggttgaatc caattcttga
      301 atgcctgctg agagctgctg tacggacgcg gatacgtctt ctaattgtgc cgaaatcgtt
      361 ttggtgtttt tcaaaatgac atgaaacttt tgggacgttt cttgcgacgt gttcattccg
      421 ctttgaacgt tttgaatgac gtctcccatg atctgggcag tgttttcgat atcttgctgt
      481 atgcgagcaa ttaaatgagc aatctgtttc gttgactctt gggactgttc ggccagtttt
      541 ctcacttcat cggcgaccac cgcaaacccc gctccgtgtt ctccagctct tgccgcttca
      601 atcgcggcgt ttaaagcgag gagatttgtc tgttcagcga tttggttgat gatgttgacg
      661 atcgattcga tttcttttga ccgctcctga agggagcgaa tgactttttc tgattggagg
      721 acagactgtt gaatggcgtt catctgttct acggtttttt ccacagattt tcccccatcc
      781 tccgcttggg caatcatatg gacagacaaa tcggctatgg ctgtcgaacg atctacgata
      841 ctggccactc cttttgccat ttgttccatg gatgcggccg ttgcgtctgt tttgctgctc
      901 tggatgtctg ccctgttcgc tacttcttga atgcctgcgg aaacttgttc ggtcgacaga
      961 acggtttgtt ccgcgctggc tgtcagttcc tcggcagctg cggcgacttg ctctgacgcg
     1021 gctgtgacgc gtgcgagcaa ctcggaaagg ttgtctctca ttttattgta gctttcaaca
     1081 atttgtccga tttcatcgcg tgcaccatcg ttcagtgtga cagtaaagtc tccttccccg
     1141 gcttgctgga gcgccttgga gacagcatct aatctgacgc ggatgtgtcg tgtgaaccaa
     1201 accgtaacgc ttgcagcgat cgctaatacg gcaacagcag aaagaagcag cattctgaaa
     1261 agcgaagaca ctaccgtgtt cacatgtttt tggggaattc cgacataaaa catccctata
     1321 atctccccat tatggctctt aataggcata tatgccgctt gataccattc acctaaaatt
     1381 tcgacttccc ggaaaaaggt gcctccttcc tttaacactt tgttgacgac tggctttgga
     1441 gccgttgtgc caattgctcg ttggccatga aggatgatat tagtcgctac tcgtgtatcg
     1501 ttttgaaaaa tagtgaccgt tccgccaatc atttttccga tttcgtcaac cagctgattg
     1561 tcgccattga tttgggtttc tcctttatag atcattccgt cttttaacat ccaatttcct
     1621 ttgtacttct cgtctatgta acggtaagcc aatataaggt ttgatttgac tctttccagc
     1681 gtttcttttt ttattccttc tttgatctgg atgcttgcga ccgtcataat cactgtggtc
     1741 aataaaacga tcgtacagga aaacatgaca ttcatctttg tccccagttt gaacgatttc
     1801 aataaacctt ccccccctag tttcttcaaa tatgtctata attatagtta tgaagccctg
     1861 cttgataaat agtattgcat tcctaatcgt ctatgttagt tgataaggat agcgtttgcg
     1921 tctttcgata cacgatcatg catttgatcg atagctgggg ggcgcaattt ccggaaaggc
     1981 agacagcctg ctttttttgg agattcagga atgatagaaa agactttgat ccaattttat
     2041 ttctttaaac ttctataaat attaaacttc tataaataaa agggaatctt agtttattgc
     2101 catttaatca tgaggcgggg gaatgcgcct tggcaacagc aaatccattg accggttttt
     2161 acacaaaaat tttaggatcc gcatccaggc aaagtggaag ttataaaaat tgaacctatt
     2221 gtaatttcgt atcctctcga caaaaattga gaagattttt atcgagagga agtactgaaa
     2281 acgatcaaca gctgctatat agtaaaaaat tttagtaaaa acattgaatg tctgttataa
     2341 taataatgga caaaactgag tagaatgggg taatgcacat ggccacaatt cgcgacattg
     2401 ccaaacaagc aggctattca atttcaactg tctcgcgggt gttgaacaat gatccagatc
     2461 tttcggtatc tgaggagacg cgggagaaaa tttatgaagt agcagaaaag ttgaactacc
     2521 gcaagaaaac catcagaaaa gtgattcgcg atattgcttt tttatattgg ttgacggata
     2581 aagaggaatt agaagatgtg tattacaaaa caatgaggat ggaaatggaa aagctggcga
     2641 aagagtttaa tctagagctg cagatgtata aaatttcaga tggcattgac caaattccag
     2701 agaacattga ggggtttatc gccgttggaa ttttttcgga tcgggaactt gagcgtctgc
     2761 gatcgattac gacgaatgga gtatttattg atttatcgcc cgacccgcgc tgttttgatt
     2821 cggtgcgccc ggatttaaca cagatcactg agcgggcgat cgacttcttt ctcgagaaag
     2881 ggcatacgga catcggcttt attggcggga cgtatccgaa tccgaataca ggaaaggatg
     2941 agatggactc gcgcgagcgg gcgtttcgaa attatatgaa agcaaagggg ttgctcaagg
     3001 aagagtatat tttttgccac cgagggtttt cttttgacaa tggctatgaa ctcatgacca
     3061 aggcgattga aaccctgggg gatcgattgc ctaccgcgtt ttttgtggcg gctgatccga
     3121 ttgccgtcgg ttgcctgcag gcgttgaatg agcatcgcat tgccatccct gacagggtga
     3181 gcgtcatcag tgtcaataac atcagtatta ccaaatatgt ttcgccgccg ttgacgacat
     3241 ttcatattga tattcatgaa atttgcaaaa gtgcgatcga gttgttgctg gagcggatac
     3301 aaagaaagag aaaaattgtc aagacattgc tgttaggggc tgaactcatc gtacgaaaaa
     3361 gtacgaacta gcctcttttt tgtttcgtaa atatttttat taattttagt aaaaagttgt
     3421 ttacattttt tattgttggt gctatattga aggtagttaa gcgtttccaa aagagaagga
     3481 ggaatggggg aaatgatgag gagtattagt aaacatcttg cacttgtgag tgtgatggct
     3541 tttagtttgt ctttagctgc atgtgggcct aaagaaaatg ctaatcaagg atctcgtgcg
     3601 ggagaaaaca aagagaacaa tggaaaagta gagctcgtcg tttgggaaga cattcaaaaa
     3661 agcgcgggaa ttaaagatgc cattgcccaa tttgaaaagg aaaacaatgt gaaagtaaaa
     3721 gtcattgaga agccgtatgc gaaacaaatt gaggatttgc gtttggacgg gccggccggc
     3781 actgggccgg atgtattgac gatgccgagc gaccagattg ggacagccgt gacggaaggg
     3841 ctcattaaag aattgaatgt tggtgaagac attcaatcga tctacacgga agccgccatg
     3901 cagtcgcaaa aagtaaacaa caaagtgtat ggtctgccaa aagcggtcga gacaacgacg
     3961 ctttactaca acaaagatct catctctgaa gaggaattgc cgaaaacgct tgaggaatgg
     4021 tatgagtatt caaagaaagt gacagatggt aaaaaattcg gctttttggc gctgtttgac
     4081 cagatttact acgcccaaag tgtgatgagc ggatatggtg gttatatttt tggcaaggac
     4141 gaaaatggga attataatcc gaaagatatt ggattaaaca atgccggcgc tgttgaagga
     4201 gctaaaatta ttcaaaagtt ttataaagaa ggactgttcc catcggggat gattggggag
     4261 caggggatta atgtgctcga atcgctgttt acggaaggca aagcagtagc gatcatttct
     4321 ggtccgtgga atattgagcc gttcacaaaa gcaggaatca actttggggt gacgaaactc
     4381 ccagctttag aaaacggaaa aaatatgagc gcgtttattg gggtaaaaag ctacaacgta
     4441 agtgcctatt caaaacatcc agaccttgct gagaaattgg tcatcttcct agctaacgaa
     4501 gaaaattcga aaaagcgata tgaagtgaca aaagaggttc ctgctgtcaa agctttagtg
     4561 aatgatccga ttgtgaaaaa cagcgaggcc gcacgtgcgg ttgccgagca gtctcagttt
     4621 tctgaaatga ttccgaacat tccggaaatg aacgaagtat ggaaaccggc tgattcggca
     4681 ttacaaacga ttgcgactgg aaaggcggag ccaaaagtag cgctggatca ggcagtaaaa
     4741 acgattaaag ggcaaattga ggccaaacat agcggcaaat aaggggcgtc gcaaaaggtc
     4801 cttgcgcctt gttatatcaa agagccaaag cgcaagggcc taagccttgg cccataaatg
     4861 aatcgatcgt gatggtgtta aacagccgtt ttgtttgaca ccttttgttt tcgagaaaaa
     4921 atgtgttcgg atttgccaag gaaagagggg aaaaagatgc cgcatcggaa aatcgcattg
     4981 tttttatcca tcattcccgg cctcggtcag ctttacaatc gtcaatgggc gaaaggaatc
     5041 ttatttctcg ttttctctgt atcttttttg atcgtctttg ccgatctttt caatatgggg
     5101 ttttggggga ttgtgacgct aggaacggaa gtgcagcggg ataattcgat ctttcttttg
     5161 gcggaaggaa ttattgcggt gattgtcgct gttttcgggt taacttttta ttattttaat
     5221 ttgcgggatg cctataaaaa cggcgagcgg agagaccgag ggattagatt aagcactctt
     5281 aaggaagact atcatcagct cattgagcaa ggctatccat atgtcattag cagccctgcc
     5341 ttttttctct tgattttttc ggtgattttt cctgtgttgt tcagctttgc attagctttc
     5401 acgaactatg atttatacca ttctcctcct gcccgtttgg tcgactgggt tggttttgat
     5461 acatttttga aaattttcac ggttgatatt tggcgatcaa cattttttga tgtgttggcg
     5521 tggaccgttg tttggacggt tgtcgcctcc actcttcaag tggcgattgg aattttgttg
     5581 gctgttgttg tcaatcagaa agaccttcgc ttgaagaact tttatcgtac gattttcgtt
     5641 ttgccatggg ccgtgccggg gtttgtcacg attttagtgt ttgccggctt gtttaatgat
     5701 agttttggag cgatcaacca tacgatttta gcggcgcttg gcatcgatcc aatcccttgg
     5761 atgaccgatg agcattggac gagggtggcg ctcatcctca tgcaaggatg gttgggtttc
     5821 ccatatgtgt ttgtcgttac gacgggcgtt ttgcagtcga ttcctgaaga gctgtatgaa
     5881 gcagcgacga ttgacggagc ttccgtgttt gccaaatttc gatatattac attgccgatg
     5941 gtgttgttgg cgatggcgcc gattattatc acacagttta catttaactt taataacttc
     6001 aacattattt atctgtttaa tggaggcgga ccagctgttc cgggatcgac agcgggggga
     6061 acggatattc ttgtgtcttg gatttataaa ttgacgatgc aatcaagcca atatgcgttg
     6121 gctgccgctt taacgatctt gttatctttg tttgtcatgg ggattgcgtt atggcaattt
     6181 cgacggacca attcttttaa agagggagta tagaacatga gagccagtgt gaggtcaaaa
     6241 aagttccttc gcctctttgt atcctatgcc attttaacgt ttgtcgccat ggcgatcatc
     6301 tatccgctgc tttggacaat tggcgccagt ctgaatccgg ggaacagctt aattagcacg
     6361 actcttattc cgaataaccc tacttttgac cattatcgtg aattgtttgc tggcaaggaa
     6421 agcctgccgt atgcacgttg gtattggaat tcgatgaaaa tcagtttttt tacaatgatc
     6481 ggttccgtta tcagcgtttc atttacggcc tatgcttttt cacgatttcg ctttaaaggg
     6541 agaaaaaatg cgttaacgtt atttttgttg ctgcaaatgg tcccacaatt ttcagcattg
     6601 attgccttat ttgttttggc gcaaatgctc gggatgatca atagtcactg gctgttaatt
     6661 ttgctttata tcggcgggca aattccgatg aatacgtact tgatgaaggg atatatggat
     6721 tcgatcccga gagatttgga cgaaagcgcc aaaatcgatg gggcgagcag tacgaggatc
     6781 tttttccaaa ttcttttgcc gttatctaag ccaatgattg cggtcgtggc gatgaacgga
     6841 tttaccggac cgcttggcga ttttgtttta tcatcggcca ttctccgaac tcctgatgct
     6901 tatacgcttc cgatagggct gtataatctc gttaacgaag tgatgggggc gagctataca
     6961 acgtttgcag ccggggcgct tctgattagc attcccgtag cgattgtctt tttgatgttg
     7021 caacggaact ttgtgtccgg attgacattg ggtggaatca aaggatgatg cagtgaaagg
     7081 ggacgacatg atgccaaaat atgaacgaac gtatacaaca caagcgaact tcattcttca
     7141 tggaggagac tacaaccctg atcaatggct ggatcggccg gatattttgc aggcggatct
     7201 tgagttgatg aaactctccc atacaaacac attcacggtt ggtgtgtttg cttggtctgc
     7261 tctcgaaccg gaagaagggg tttatcgttt tgaatggctc gataaagtgt ttgacgacat
     7321 ttaccgtatt ggcgggcggg tgattttggc cacgccaagc ggcgcccggc cagcatggtt
     7381 gtcgcaaaag tatccggaag tgttgcgggt gaatgcggcg cgtgttcgac agttgcatgg
     7441 tgggcggcat aatcattgtt ttacctcgag cgtataccgc gaaaaaactc aacatatcaa
     7501 tcgattgctg gcggaacggt acggagatca tcctgctttg ctcatgtggc acgtttccaa
     7561 cgaatacggc ggggaatgcc attgtaatct gtgccaagag gcgtttcgcg aatggttgaa
     7621 aaagaaatac aatcatgacc ttgacgcgct gaatgccgct tggtggacat cgttttggag
     7681 ccatacgtac accgactggt cgcaaatcga gtcgccgtct ccgattggtg aacatacgat
     7741 tcatgggttg aatcttgatt ggaaacggtt tgtgacagat cagacgattt ccttttttga
     7801 aaacgagatc gttccgcttc gggagttgac accgcatatt ccgattacaa cgaattttat
     7861 ggcggatacg catgacttaa ttccttttca agggcttgac tacagcaagt ttgcgaaaca
     7921 tcttgatgtc attagttggg acgcctaccc tgcttggcac aatgattggg aaagcaccgc
     7981 cgatttggcc atgaaagtcg gttttatcaa tgatttatat cgaagtctga agcagcagcc
     8041 gtttttattg atggaatgca cgccgagttt agtgaactgg cataaagtga acaaagcgaa
     8101 gcggccaggc atgcactttt tatcttcgat gcaaatgatc gcccatggtt ccgacagcat
     8161 cctctatttt caatggcgca agtcgcgggg atcgtttgag aagtttcatg gcgcggttgt
     8221 cgaccacgac aatcgaacag acagccgtgt atttcaagaa gtcgccgaag tcggaaaggc
     8281 gctcaagaag atgtctggca tcgtcggaac caaccggccg gcggaagtgg cgattttgta
     8341 tgactgggaa aacaattggg cgttaaatga tgcgcaaggg tttgcggccg aaacgaagcg
     8401 ctatccgcaa acactagtac aacattatcg tccgttttgg gagcgtgata ttcctgttga
     8461 tgtaatcaca aaagagcatg atttttcccg ctacaagttg cttattgcgc cgatgcttta
     8521 cttagtgagc gaagagacga ttgctcgttt gaaagagttt gtagcgaacg gtgggacgct
     8581 tgtcatgacc tatataagcg ggatcgtcga cgaacatgat ttagcgtatt taggaggttg
     8641 gcatcaagac ttgagggaaa tgttcggcat ggagccgata gaaacggata cactttatcc
     8701 acgcgatcgg aattcggttc actatcgtgg acgatcttac gagctgaaag actatgcaac
     8761 ggtgatcaag attcatgccg caacagtcga aggagtttat gaagatgatt tttatgcgga
     8821 tacgcccgct gtgacgagca accaatacgg gaaaggccaa gcctattata ttggcggccg
     8881 cctagaggat caattccata gagattttta tcaagaattg atggaaaaac tggatctgag
     8941 gcctgtcttg tttgtaaaac atgaaaaagg agtatccgtg caagcgcggc aagcgccgga
     9001 atgtgattat gtgtttatta tgaattttac ggaggaaaaa caggctgtag ttcttgagga
     9061 aaaggtgaag gatctcttca ctggtgagga aattgtaggg gagattatgc tggataaata
     9121 cgaggtgcgg gtcgtggaga aaaggagata aaggagagag gttcgttcaa gcaacaaatc
     9181 tatcagatag cagacaggac ggtcaggttc cataaggtag tcaatcaagt tttttgggtg
     9241 cgttatgttc tgaaaatatt catggaggcg ggggagagga tgggaaaaca atttcttttc
     9301 gtactagtcg cgatgatggt gatgatcatg tcgttttcgt ccaatcatat tcataaagca
     9361 gcggctgatt cctataatta tttaattaac gggggatttg aatctgattt ttgggctgat
     9421 gggtcatgga cagtcactac acaggattgg agttctgttg atctacagcg tttttcttat
     9481 tcaaatgatg catggatcgt tccgaatgaa ggagatagcg cctttaaata ctggattcgt
     9541 gatatagcga acggaaatca aacattcacg ttgaaacaaa cgatcggaca gctgccgcca
     9601 gggagttatg aactgtccgt tcaatcgatg ggaggagcag gagacgaagc gggttatatt
     9661 caattgttcg cgggtgataa gatggcggat gctgttgtta caaaaggata taattcttgg
     9721 gagacggtta aattggaatt cactctggaa gaagaggcat ctaactttga agtcggtgca
     9781 atagttactg gggaaccaaa cgcttggggc tatatggatc attttcagtt ggtatcgctg
     9841 aacagcgcgg gaggcagaga gatgccaaag ccggttccgg ctgatatttt cgtaaaaagg
     9901 gtggatggcc taagcaaaga gttcattaaa ggggtggatg tttctagcat tatcgcactt
     9961 gaggacagcg gggtgaaatt ttacaatgcg gccggtaaga agcaagatat ttttaaaacg
    10021 ctaaaagaag ctgggatcaa ttatgtgcgg gtgcgcgtat ggaacgatcc ttatgatgcc
    10081 aaagggcatg gctatggagg cgggaataat gatttggaaa aggccattga gattggaaaa
    10141 cgggctacgg ccagcgggat gaagctacta gtggacttcc actattccga tttttgggcc
    10201 gatcctgcca aacaacgccc tccaaaagca tgggcaaaac tcaattttga agcgaaaaag
    10261 aaagctttat accgattcac gaaagaaagt ttaaaagcaa tgttgaaaga aaagatccaa
    10321 gtcggcatgg tgcagattgg aaatgaaaca aacggggctt ttgtcggtga aacggattgg
    10381 gctaaaatat gtgagttgtt gaatgcaggg agccgggcgg taagggagac gagtccgaat
    10441 atattggtgg tgctgcattt cactaatccc gaaactccag gcagatatgc atccatagct
    10501 aaaacgcttg ctgaacataa agtagattac gatgtgttcg ccagttccta ttacccgttt
    10561 tggcatggta ctttagccaa tttaacgtct gtgttgaaat atgtagccaa tacttacctc
    10621 aaaaaggtta tggtggcaga aacatcctat ccgtatacga cagaagatgg agacggccat
    10681 gaaaatacag caccaaagag ttccggacaa acattgaact atcctattac cgttcaagga
    10741 caagcgaacg cgattagaga tgtcattcaa gcggtggcag ctgtaggcaa accggggatc
    10801 ggtgtatttt attgggaacc agcatggatt cctgtcgggc ctcctgagca actaaagcaa
    10861 aatgaaaaga aatgggaaaa atacggatct ggttgggcgt ctagttttgc agctgaatat
    10921 gaccctgatg cggcgatgtg gcatggggga agtgccgtag ataatcaagc tttgtttgat
    10981 tttaatgggc gccctttgcc gtcgttaaat gtgtttaagt atgtggatac aggagctgtg
    11041 gccccattaa aaatcgatga gatcaaagat atatatattc gtgctgcgtt cggtgaacag
    11101 gttgttttgc ctacgacggt aactgccaca tataatgacg ggagcaaaca ccttgtttct
    11161 gtgaaatggg accaagccgc gttggaacaa gcactgagaa ccggcgtcgg aacatatgtg
    11221 attcaaggtg tagtggaagg tggagagacg gtaaaagccc atcttgaaat tacaccgaaa
    11281 aactatgtag tcaatccgag cttcgaggat caagacagaa cgatgtggaa catcatttat
    11341 cgaaacggca cctctccaca tacggattat ttggagaaag ctacggatgc gaaatcagga
    11401 aactattccc ttcacttcta ttcgaatgag gctgttgatt tccaagtaga acagacgatt
    11461 acgggattga agccgggata ttacaattta tcgatgtttt tgcaagcagg ggatgccccg
    11521 aatgcagaaa tatatttgta tgctaaaaca ggagacaaag agtatcgagc ggctgcctcg
    11581 gtgaacggat ggttgaattg gagcagcccc gaaatccatg atatttatat cgctgacgga
    11641 acggtgacga ttggtgcgag catcaaggca aatggcggag catgggggac actggacgat
    11701 tttcagttat accaaacaaa gtaattaacg aagcttccgc tgcttttcaa gacggtgaaa
    11761 caacgggaaa tttaagttgt tttctttcgt tatgaagatc aaccgggcga aaaaaatcgc
    11821 ccggtttttc tttgatgagg tggaaaatta atggactatg tgcggcaatc aacatcacgc
    11881 ctacgctttc tgcacacctt cctgccattc atgagggtta tattgaaatc tagcatgatc
    11941 aagaagctgg ctagctccga ccgctcttga ccgcaatgcg gggaaagtgg aagccgttca
    12001 cgccgcccta tgccatagac gaatgacaaa gctagctagc aaaatcagaa cgacggaacc
    12061 gatcagagca gggatgatgt caaatccgcc cacagtcggc cctcagttgc ccaatagcgc
    12121 tgtgccaagc catgctccga taaacccggc gatgatgttg ccgatgattc cgcctggaat
    12181 gtcacgtcct gtcatcaaac ctgccaacca accgatgacg cccccgacga ttaagctcca
    12241 aagaaacgac atgatgcatt cctcccgatg tgtaaaatct tttcctccta cattaattat
    12301 tatgtgcatc agaacgaaag aaattatgcg attgatatgt ccattgcatc ggaggaaacg
    12361 atcttgtcac catcatgcat gattgttttt agcatggtct atgttgaccg cggcttgccg
    12421 cacaggcgca tggatcaaat gttgttatga tgtccgtaaa tcgttagctt cttctctacc
    12481 tttgtgcgcg gccgggcagg agggatttgg tcgggatcga agaagccgag atgcaaaaat
    12541 caaagccgcg cttgactcga gtcaaacgcg gctttcggat gaaggaaaac tagcgaatga
    12601 attcaagctg cttggcaaga tggtggagtt ttttctcatg ctggatggtt ttggacgcga
    12661 ggaaatggac ggtttcttgc gtttctgtca gctccacgcg caagccggcg aatttttgct
    12721 cgagtcggtc gaaccgctcg ttcacctcgg cgcgaaattg ctgcatttcc gccctcagct
    12781 gattagcagt gtcttggatt tccgccctca gctggcttcc catttcgtca atttgggtgc
    12841 ggatgtcgcg ggagaatatt ttcagcgcct caaccacttc tttcacaacc gtatcgcgtt
    12901 ccatgcttgt tcacctcctc ttttttgatt atacaacgtg gagcagggaa agaaaactat
    12961 gttttgaaca agctttttct ccgttttcat ttgattgatc gttggctcga gttgcggcgt
    13021 cgtgggtgag gttggaaagt tcatgtcaat gatcatggcg gcgttttgag ggaacgggtg
    13081 gatctgcctc ttcgttaatc aaaatatta
//
