LOCUS       CP000033               14963 bp    DNA     linear   BCT 30-JAN-2014
DEFINITION  Lactobacillus acidophilus NCFM, complete genome.
ACCESSION   CP000033
VERSION     CP000033.3
DBLINK      BioProject: PRJNA82
            BioSample: SAMN02603047
KEYWORDS    .
SOURCE      Lactobacillus acidophilus NCFM
  ORGANISM  Lactobacillus acidophilus NCFM
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactobacillus.
REFERENCE   1  (bases 1 to 14963)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Complete genome sequence of the probiotic lactic acid bacterium
            Lactobacillus acidophilus NCFM
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (11), 3906-3912 (2005)
   PUBMED   15671160
REFERENCE   2  (bases 1 to 14963)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
REFERENCE   3  (bases 1 to 14963)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2007) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 14963)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2007) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
  REMARK    Sequence update by submitter
COMMENT     On Nov 1, 2007 this sequence version replaced CP000033.2.
FEATURES             Location/Qualifiers
     source          1..14963
                     /organism="Lactobacillus acidophilus NCFM"
                     /mol_type="genomic DNA"
                     /strain="NCFM"
                     /db_xref="taxon:272621"
     gene            1..940
                     /gene="trxB"
                     /locus_tag="LBA0679"
     regulatory      1..6
                     /regulatory_class="ribosome_binding_site"
                     /gene="trxB"
                     /locus_tag="LBA0679"
     CDS             14..940
                     /gene="trxB"
                     /locus_tag="LBA0679"
                     /EC_number="1.8.1.9"
                     /note="TrxB; COG0492 Thioredoxin reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|Pyr_redox_2"
                     /protein_id="AAV42555.1"
                     /translation="MADKYDVIVIGAGPGGMTAALYATRANLNVLVLDRGPYGGQMNNT
                     DAIDNYPGFTEVKGPELSQKMYDTLMKFEPDYKYGNVQSVELDGDEKVIKTDDEEYRAP
                     VLIIATGADHRHLNVPGEEEYSGRGVSYCAVCDAAFFKDEDVVVVGGGDSAIEEGIYLS
                     QMAKSVTVIHRRDQLRAQPTLQKRAFANEKMKFIWNAQTEEILGDGNRVTGVKYLDKEN
                     GETKEVSAAGVFIYVGVLPQTEPFKKLGILNEQGWIPTNEHMETKVSGIFALGDVRDKD
                     LRQIANAVGEGSVAGQAAYNYFQDLKN"
     gene            1105..3034
                     /gene="glgB"
                     /locus_tag="LBA0680"
     regulatory      1105..1110
                     /regulatory_class="ribosome_binding_site"
                     /gene="glgB"
                     /locus_tag="LBA0680"
     CDS             1118..3034
                     /gene="glgB"
                     /locus_tag="LBA0680"
                     /EC_number="2.4.1.18"
                     /note="GlgB; hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13_9|CBM48|GH13"
                     /protein_id="AAV42556.1"
                     /translation="MVTIVEVKQHIKKFAAGNELYLQEVLGCHYENDIYTFRVWAPNAQ
                     KVWLVGDFNDWDKSLEMSQTLDGVWEIKTSLPKEGQLYKFLVKQADGREVMKIDPMAFE
                     LEPRPGSAAVIVKLPNKKWLDGAWMGRNKRSNHFARPINIYEVHASSWKRHTDGSLYTL
                     KDLQKELIPYVKEQGFNYIEFLPLTAHPLDASWGYQTIGYYALERTYGTPRELQDFVEA
                     CHKENIGVLADWVPGHFCINDDALAYYDGTPCYEFSEKWRAENKGWGALNFDLGKPEVQ
                     SFLLSSALFWLEFYHLDGLRVDAVSNMIYRDYDRSDGEWKTDKFGGNRNLEGIEFLQKL
                     NRTIKGKHPECLMIAEESSAQVKITGRIEDGGLGFDFKWNMGWMNDILRFYEMDPLFRK
                     FNFNLATFSFMYRMSENFILPLSHDEVVHGKRSLMNKMFGDRDKQFAQLRNLLTLQMTY
                     PGKKLLFMGSEFGQYLEWRYNDGLDWAELKDELNAKMKHFDQDLNSFYLNEPALWQLEQ
                     REDSVQIIDADNKDESVLSFIRQGKTRHDFLIVILNFTPVDRKKITIGVPYAGKYCEVF
                     NSARKEYGGSWNQEKQNLKTQNNSFKNFNYQVQLDIPGFSAVILKPVDVHIKRRINRKT
                     KTK"
     gene            3037..4195
                     /gene="glgC"
                     /locus_tag="LBA0681"
     regulatory      3037..3043
                     /regulatory_class="ribosome_binding_site"
                     /gene="glgC"
                     /locus_tag="LBA0681"
     CDS             3050..4195
                     /gene="glgC"
                     /locus_tag="LBA0681"
                     /EC_number="2.7.7.27"
                     /note="GlgC"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="AAV42557.1"
                     /translation="MSTEMLGLILAGGKGTRLGKLTSDQAKPAVPFGGRYRIIDFTLSN
                     CANSGVKNIGIITQYQPLLLNEHIGNGVSWGLDGLNASATILQPYTDNSGSKWFEGTAH
                     AIYQNIDYIDSKDPEYVLILSGDHIYKMDYESMLEEHIKNGASLTVAVIDVPMKEASRF
                     GIMNTDVSGRITEFEEKPEHPKSNHASMGIYIFNWKRLREVLTTGFTTNDDMVDFGKNV
                     IPYYLKSDERVFAYHFSGYWKDVGTIDSLWAANMEFLDGSDGLNLYDRSWRIYSKNPID
                     PAQVITENAEVNNSMIVDGCYVDGTINHSILSTDVDVQRGSEITDSVIMPGVKIGKDVV
                     IKHAIVGENAQIGDGAVVEGNKDNIKVIGNSERIGVLPNED"
     gene            4185..5327
                     /gene="glgD"
                     /locus_tag="LBA0682"
     CDS             4185..5327
                     /gene="glgD"
                     /locus_tag="LBA0682"
                     /EC_number="2.7.7.27"
                     /note="GlgD; Required for glycogen biosynthesis"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="AAV42558.1"
                     /translation="MKTNKMAAIFSNQHEYNELRPLTDERSLSALYFAGKYRIMDFALS
                     SIVNAGINNVYTLISQEKVRSYLDHLGGGKEWGLDTIGSYAYLDFYQKLLQSQANGGEY
                     FNDLITFLATANAPYTVFLENKIVANVDLQSVLHFHQENGNRITAVFKRVSKKNVAPDD
                     QLYILDEENTIMSCQQAIDTQSQDSYNLGLNIYVADTKWLIAELKKAQKCGAPIDIGQR
                     FASLAAKYRTNAYEYTGYLHNVHDIKSYYDSNIEMLDKKNRDSLLHGNQRIITRIRNEV
                     GTYYTTSSKVKNSLFATGCRINGTVENSILSRRIDVAEDSNVKNSIVMASCTLEAGSSV
                     EYAILDKNVVIKKDVVVKGTPDNPVVIKKGDVVTKDLIVE"
     gene            5327..6767
                     /locus_tag="LBA0683"
     regulatory      5327..5332
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA0683"
     CDS             5337..6767
                     /locus_tag="LBA0683"
                     /EC_number="2.4.1.21"
                     /codon_start=1
                     /transl_table=11
                     /product="GT5"
                     /protein_id="AAV42559.1"
                     /translation="MRVLFTGAECAPFFKTGGLGDVLGSLPNQLAKEGVDVGVVLPLYQ
                     DLPEKYRKNLKYQGNFIVPVGWRNQYCGIFTLKLNGVNYFFIDNEYYFKRPGIYGYYDD
                     GERYAYFQQAVIMMMERFDFIPDVLHCNDYHTAFIPFLLHEKWGFVDAYKGIKTILTIH
                     NLEFQGKYNAKTLPDFFGMNYDWFDSGIVRMDNDVNWMKTGILYADRVTTVSPSYAREI
                     QTVEFGQGLDAILRMCSHKLTGILNGIDFEKYNPKTDPVIKKNYDVYHLHHKSKDKIAL
                     QKELDLPIKPNIPLIGMVSRLTAQKGCQLLLDELDNILQFNVQIVILGNGDPYYEHRLM
                     EIAERYPDKLKVILAFDVKLAQRIYAGADSFLMPSAFEPCGLSQLIALRYGTLPIVHEI
                     GGLADTVWVYDETTNEGTGFGFKEFSGYQMVQAIKKMLALYQQKNNWLKIQKIAMKSDF
                     SWKNSADKYKWMYGELIG"
     gene            6786..9197
                     /locus_tag="LBA0685"
     CDS             6786..9197
                     /locus_tag="LBA0685"
                     /EC_number="2.4.1.1"
                     /codon_start=1
                     /transl_table=11
                     /product="GT35"
                     /protein_id="AAV42560.1"
                     /translation="MQLTKDEFKKKLKNKVNIYFEEELDEASNSELYTALSGVVRDGYA
                     PQWRRTRISEADQGQKQVYYFSIEFLPGTLLKTNLLNLGWLDTVRAALSDLGLDLDKIA
                     AAEPDMALGNGGLGRLAAAFMDSLASTGYTGNGNGIRYKYGLFKQKFVNGYQKELPNDW
                     LKKDDHWEVRRESKSVLVRFGGKVRMVDDNGWMTPQYEGGDVVRAVPYDTAIVGYRDGV
                     TNTLRLWDAEIPPEEELSYPTISDRRRIEDLTSILYPDDSNYEGRLLRLKQEYFFVSAG
                     LQSILDYYVKKLGNKDFTKLPDYVAVHINDTHPAMAIAELMRLLVDEHRVDWETAWDIT
                     LKVMSYTNHTIMSEAMEKWDTNMLSQLLPRIMQIITEIDRRYCAYLNGQVSNDVIERTR
                     IIKNGQVQMAHLAIIGSHSINGVAALHTQLLETKVLKDFYNLYPDRFNNKTNGITLRRW
                     LQIANPELSDLLDQTIGKDWRKNSDKMLNFEKYYNDTLVLERINQIKLDNKKKLAEFIK
                     EQMGVEVDPNAIFDVQVKRLHEYKRQTLKLLHILKLYQDLKAGIDHPKRVVIFGAKAAP
                     SYVFAKQVIKVINETANMINSDPDINGKLKVIFLENYDVSLAEKIIPAADVSEQISTTT
                     KEASGTSNMKLMANGALTVATMDGANIEIADAVGEDNIITFGLNKDQVYKYYAEHSYHP
                     REMYESDPVMKKTVDALTDGTIPNCFSEGQALANKFLSDNEQFFVLADFADYLKAQEKV
                     EQEWKDKHSWAQMSLVNIAHSERFDVDKTIERYAQDIWHLKKLKVEKVDH"
     gene            9197..10966
                     /locus_tag="LBA0686"
     CDS             9197..10966
                     /locus_tag="LBA0686"
                     /EC_number="3.2.1.41"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13_39|CBM34|GH13"
                     /protein_id="AAV42561.1"
                     /translation="MKITYDSWQEQYKNPFGAVKAGNTVKWSIKIDQVIQGAVLWLTKS
                     RETPVAYPMNYDEQTKMYTTQVKIGTSGLYNYYFALQQNNQIVYIDQGLFGKGHVTKSD
                     HDLRQFQLTCYDIATPRIDWYQKGIVYQIFPDRFANGNPYEEVIGKKRNSFIYATKEDI
                     PYYIKNSEGAIVRWDFFGGNLTGIRKKIPYLKQLGVTVLYLNPIFLAKSNHRYDTTDFM
                     KIDPMLGDEKDLADLIRELHENNMHLILDGVFNHVGFDSIYFQGAITDKNSNYRSWFNF
                     QDYPNKYQSWWGVKSLPTVNKDNSEYQNLVYGDHGVLAKWKVDGWRLDVADELPMDFLR
                     NIRNRLIKENCPILIGEVWEDASNKFVNGEYRTYTAGDNLMGVMNYPIRNFIISLLSAQ
                     DSTIEIEAMNDLALLIENYPTDFLHNCLNNIGTHDTVRIKTVLNKNDNLVMMAFGLLFM
                     MPGVPCIYYGDEAGLIGKEDPDNRRYFLWGHEDKKLIDCVSSWTKIRKQNPVLVNGKIG
                     FVHLSAGVNSIVRYNDQEMIMYCVNCTNEDVIPLREKYSFYWLPSIIIDKIKDTLDQIQ
                     LKAQTDFIKKISL"
     gene            11074..12809
                     /locus_tag="LBA0687"
     regulatory      11074..11080
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA0687"
     CDS             11085..12809
                     /locus_tag="LBA0687"
                     /EC_number="5.4.2.2"
                     /note="COG1109 Phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="AAV42562.1"
                     /translation="MEAKEIFKEWQNATGLPDYLKDQLDTLGKDEKWIEDAFGQDINFG
                     TAGMRGRLEPGTNRINLFTVGRVTEGLARLIDENGEEAKKRGVVIAFDSRYHSREFAEH
                     AARVLGAHGIHVYLFDDLRPTPELSYAVRHLNTFAGINITASHNAKQYNGYKVYGEDGA
                     QMAPENADRLFAYAQKVTDIFGVEVASVKELRANNTLQLIGEDVDEAYLNELKQVTVDK
                     EMVKANADKLKIIYTPLHGTGKMLYDRAFRQGGFDNVIPVPSQSIIDPEFPTTIKPNPE
                     YRDVFEPGFKLADKVDANVIIATDPDADRMGAAVRKSDGDFQVLTGNQIATLMAYYLLA
                     HLKESGKLTDDYEIVTSVVSSALPFKIAEDFGIKTKHVLTGFKYIGEEVDRMNKAGDAK
                     FLMGFEESYGYLFKPFARDKDAMQGALMFAEVASYYASRGMTVFDGLQEIWKKYGVAYE
                     ITRAIEMPGIGGQKKMAELMSKLRNEHLTEIGGSKVVKIQDFLKQETIENGEKTPLEGF
                     PESNVLKYFLDDETWVALRPSGTEPVIKAYVGVNKENIEKAEKAAEDYQTALANLLK"
     gene            12897..14963
                     /gene="uvrB"
                     /locus_tag="LBA0688"
     regulatory      12897..12904
                     /regulatory_class="ribosome_binding_site"
                     /gene="uvrB"
                     /locus_tag="LBA0688"
     CDS             12912..14963
                     /gene="uvrB"
                     /locus_tag="LBA0688"
                     /note="UvrB"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC (subunit B)"
                     /protein_id="AAV42563.1"
                     /translation="MIKRQKDRKFELVSKFKPAGDQQQAINKLTAGFEKGYKEQILEGA
                     TGTGKTFTMANIIAKLNKPTLVITHNKTLVGQLYGEFKGFFPNNAVEYFVSYYDYYQPE
                     AYVPQSDTYIEKDSAINDEIDQLRHATTSALMERNDVIVVASVSCIYGLGDPKEYARSV
                     LMIHEGQEYERNTLLRDLVNLQYDRNDIDFQRGRFRVRGDVVEIFPAGNSNHAYRVEFF
                     GDEIDRIVEIDSLTGEVIGERESISLFPATHFMTNDEQLRRALKAISKEMKVQVKKFEG
                     EGKLLEAERIKQRTTYDMEMMGEVGYTNGIENYSRHMEGRKVGEPPYTLLDFFPDDFLI
                     LIDESHATMPEIRAMYNGDRNRKKTLIDYGFRLPSALDNRPLKLAEFEKHVNQILYVSA
                     TPGDYELERTDHKVEQIIRPTGLLDPKIEVRPIEGQIDDLVGEINKRIDRNERVFVTTL
                     TKKMAEDLTDYLKDLGIKVRYLHSDIKTLERMQILRDLRLGKFDVLIGINLLREGIDVP
                     EVSLVAILDADKEGFLRAYRPLVQTMGRAARNANGEVIMYADTITDSMKAAIEATQRRR
                     KLQEEFNKEHGIVPKTIIKPVHDVISITKPSEDSKKEKTDSFADLNFDELTAKQKKTMI
                     KNLQEQMKEAAKKLDFEEAANLRDAIMELQSSSRRPKTRKGKALNGKR"
     gene            14950..>14963
                     /gene="uvrA"
                     /locus_tag="LBA0689"
     CDS             14950..>14963
                     /gene="uvrA"
                     /locus_tag="LBA0689"
                     /note="UvrA"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|A9WBR9|3.A.1.32.3"
                     /protein_id="AAV42564.1"
                     /translation="MVNDKIVVRGAREHNLKDIDLTIPKDKLVVITGLSGSGKSSLAFD
                     TLYAEGRRRYVQSLSSYARQFLGQMDKPDVDSIDGLNPAISIDQKTTSHNPRSTVGTVT
                     EINDYLRLLWARVGHPVCPNDGTLIERQSVDQMVDRIMGLPERTKIQLLAPVVRAKRGE
                     HKEVFKRIQRAGYVRVIVDGEMHEIGEEFNLEKNKRHSIDIVVDRLIVKDSIRSRLFDS
                     TEAALRLSDGYMNVDVIDGKMLNFSEDYACPKCGFTVGEMEPRLFSFNAPFGACPDCDG
                     LGMKLEVDEDLVVPDKSLTLEEGALAPWKTSNYYSEMLKQACDALGIPMDKPFNKLTKK
                     QKDIILHGSTKPVKFNITGDFGVNATTQPFEGVMTNVERRYRRPMSKFMRDVMGKYMTE
                     LTCSTCHGKRLNEKALAVKVMGKDIAQASDLSIKKALDFFSNIELSEQETIIAKPVLKE
                     VRDRLSFLDSVGLDYLTLSRTANTLSGGEAQRIRLATQIGSNLSGVMYVLDEPSIGLHQ
                     RDNDRLIASLKRMRDLGNSLIVVEHDDETMRQADYLVDMGPGAGVYGGKVMAQGTPEEV
                     EKDPNSLTGQYLSGKKFVPVPLKRRKGNGKKITITGATENNLKDIDVSFPLGKFIVVSG
                     VSGSGKSTLVNMILKRALAQKLNNNSTKPGKYKSIRGYKNIEKIIDIDQSPIGRTPRSN
                     PATYTSVFDDIRTLFAQTNEAKMRGYTKARFSFNVKGGRCEACHGDGIIKIEMNFLPDV
                     YVPCEVCHGTRYNSETLEVTYREKNIAQVLDMTINEACKFFENIPKIERKLKTIVDVGL
                     GYVKLGQSATTLSGGEAQRMKLAAELQKLSTGKNFYILDEPTTGLHTDDIKRLLEVLQR
                     LVDEGNTVLIIEHNLDVIKNADWLIDLGPEGGEGGGTIVATGTPEEVAEVRESYTGQYL
                     KPVLRRDILLTKRAQK"
ORIGIN
        1 aggagcggcg aatatggccg ataaatatga cgtaattgta attggcgctg gaccaggtgg
       61 tatgacagcg gctttatatg ctacccgtgc taatttaaat gtattggtgc ttgatcgtgg
      121 accatacggt ggtcaaatga acaatactga tgcaattgat aattatccag gctttactga
      181 agttaaagga ccagaactta gtcaaaagat gtatgatact ttaatgaagt ttgaacctga
      241 ttataaatac ggtaatgtac aatcagttga attagatgga gacgaaaaag ttattaagac
      301 tgacgatgaa gaatatcgcg ctccagtctt aattatcgct actggtgcag atcatagaca
      361 cttgaatgtt ccaggtgaag aggaatactc aggtagagga gtttcttatt gtgctgtttg
      421 tgatgctgca ttttttaagg atgaagatgt ggttgtagtg ggtggcggtg attccgctat
      481 cgaagaagga atttatcttt ctcaaatggc caagagcgta actgttattc accgtcgtga
      541 tcaattacgt gctcagccta ctttgcaaaa acgtgcattt gcaaatgaaa aaatgaagtt
      601 catttggaat gctcaaacag aagaaattct aggtgatggt aatcgtgtaa ccggtgttaa
      661 gtatctagat aaagaaaatg gagaaactaa agaagtttct gctgcaggtg tatttattta
      721 tgtgggtgtg ttaccacaaa ctgaaccatt taaaaaacta ggtattttaa atgaacaagg
      781 ttggattcca actaatgagc acatggaaac aaaagtatca ggtatttttg ctttaggtga
      841 tgttcgtgat aaagatttgc gtcaaattgc taatgcggtt ggtgaaggta gtgtagcggg
      901 gcaggctgca tataattatt ttcaagattt aaaaaactga tataaaaatt aaaaatttta
      961 aaaataagac catattttat taataaatat ggtcttattt ttttactata catagataag
     1021 tgatatataa ttaaaagtaa aggagaatct tatttataat caaagtaagc gctttccgct
     1081 ttttgtaaac tgtaattttg attttggaga aagcagtatg gtaacgatag tggaggttaa
     1141 acaacatatt aaaaaatttg cagctggcaa tgagctatat cttcaagaag tattagggtg
     1201 tcattatgaa aatgatatct atacttttag agtatgggca ccaaatgcgc aaaaagtttg
     1261 gctagttggc gattttaatg attgggataa atcattagaa atgagtcaga ctttagatgg
     1321 agtttgggaa ataaagacca gcttacccaa agaaggacag ctatataagt ttctagttaa
     1381 acaagctgat ggtcgtgagg taatgaaaat tgatcctatg gcgttcgaat tagagcctag
     1441 accaggcagt gcagctgtga ttgttaagtt acctaataaa aagtggcttg acggtgcatg
     1501 gatgggacga aataaaagat cgaatcattt tgctcgacca attaatattt atgaagtaca
     1561 tgctagttca tggaaaagac atacagatgg gtctctgtat actttgaagg atttacaaaa
     1621 ggaattaatt ccgtatgtta aagaacaagg atttaattat atcgaatttt tgcctttaac
     1681 agcacatcct ttagatgctt catggggata tcaaacaatt ggttattatg ctttggagcg
     1741 tacttatggt acaccacgag agctgcaaga ttttgttgaa gcatgtcaca aagaaaacat
     1801 cggtgttttg gctgattggg taccaggaca cttttgtatt aatgatgatg ccttggctta
     1861 ttatgatggc acaccatgtt atgaatttag cgaaaaatgg agagccgaaa ataaaggctg
     1921 gggagctcta aattttgatt taggcaaacc ggaagtgcaa tcatttttac tttctagtgc
     1981 cctattttgg ctagaatttt atcatttgga tggcttgaga gttgatgctg tttctaacat
     2041 gatttatcgc gattatgacc gcagtgacgg tgaatggaaa acagataaat ttggtggaaa
     2101 tcgtaattta gaggggatag aattcctaca aaaactaaat cgaaccatta aaggcaaaca
     2161 tccagaatgt ttaatgattg ccgaggaaag ttctgctcaa gtaaagataa ctggtcgaat
     2221 agaagatggt gggttaggtt ttgattttaa atggaatatg ggttggatga acgatatttt
     2281 acgtttttat gagatggacc cattatttag aaaatttaat tttaatctag ctactttttc
     2341 attcatgtat cgaatgagtg aaaactttat tttaccttta tcacatgacg aggttgttca
     2401 tggtaagcga agcctaatga ataaaatgtt tggcgaccgt gataagcaat ttgctcaatt
     2461 acgtaattta cttacattgc aaatgaccta tcctggtaaa aagcttttat ttatgggaag
     2521 tgaatttgga caatatcttg aatggcgata taacgatggc ttagattggg cagaattaaa
     2581 ggatgaactg aatgctaaaa tgaagcattt tgatcaagat ttaaatagtt tttatttaaa
     2641 tgagccggct ttgtggcaat tagaacaaag agaagattct gttcaaatta ttgacgcaga
     2701 taataaagat gagtcagtct tgtcatttat tcgtcaaggt aaaactagac atgacttttt
     2761 aatagttatt ttgaatttta cacctgttga tcgaaaaaaa attacaattg gtgttccata
     2821 tgctggtaaa tattgtgaag tctttaatag tgccagaaaa gaatatggtg gcagttggaa
     2881 ccaagaaaag cagaatttga aaactcaaaa taattcattt aagaatttta attatcaagt
     2941 gcaattggat ataccgggtt ttagtgctgt gattttaaaa cctgtagatg ttcatataaa
     3001 aagaagaatt aatagaaaaa caaaaactaa gtaataagga gaaaaaacaa tgagtaccga
     3061 aatgttgggc ttgattttgg caggaggaaa aggaacaaga ttaggtaaat taaccagtga
     3121 tcaagcaaaa ccagctgtcc cttttggtgg ccgatataga attattgatt ttactttaag
     3181 taattgtgct aattctggtg taaaaaatat aggaatcatt acacaatatc aaccattatt
     3241 gcttaatgag catattggta atggagtaag ctggggatta gatggattaa atgcaagtgc
     3301 cacaatctta caaccatata ctgacaattc tggtagtaaa tggtttgagg gcacggctca
     3361 tgccatttat caaaatattg attatattga tagtaaggat ccagaatatg tattaattct
     3421 ttccggtgat catatttata aaatggatta cgaatctatg ttggaagaac atattaagaa
     3481 cggtgcgtca ttgaccgtag ctgtaattga tgttcctatg aaagaagcat ctcgttttgg
     3541 aattatgaat acggatgtta gtggtagaat tacagaattt gaagaaaagc ctgagcatcc
     3601 aaaaagtaac catgcatcaa tgggaattta catctttaat tggaagagat tgcgtgaagt
     3661 tttgaccacc ggattcacta ctaatgatga tatggtggat tttggtaaaa atgttattcc
     3721 atattattta aagagcgatg aaagagtatt tgcatatcat ttttctggtt attggaaaga
     3781 tgtaggaact attgattcct tatgggctgc aaacatggaa tttttagatg gcagtgatgg
     3841 tcttaactta tatgatcgtt cctggagaat atattctaaa aatccaattg atcctgcaca
     3901 agttattact gagaatgcag aagttaacaa ttcaatgatt gtagatggat gctatgtaga
     3961 tggtacgatt aatcattcga ttttatcgac agatgttgat gtacaacgtg gctctgaaat
     4021 tactgattct gtgattatgc caggagtaaa aataggtaag gacgttgtta ttaagcacgc
     4081 gattgttggt gagaatgctc agattgggga cggagctgta gttgagggga ataaagataa
     4141 cattaaagtg ataggtaatt cagaaagaat tggagtattg ccaaatgaag actaataaaa
     4201 tggctgcaat ttttagtaat caacatgagt ataatgaatt acgcccgttg actgacgaac
     4261 gttcattatc tgctttatat ttcgcaggta aatatcgcat tatggatttt gctctttcaa
     4321 gtattgtaaa tgcaggaatt aataatgtgt ataccctgat tagtcaagaa aaagtaagat
     4381 catatcttga tcatttaggc ggaggcaaag aatgggggct agatactata ggctcttatg
     4441 catatcttga tttttatcaa aaattattgc aaagccaagc aaatggagga gaatatttta
     4501 atgatttaat taccttcttg gctacagcta atgctccata tactgttttt cttgaaaata
     4561 aaatagttgc taatgttgat ttgcagtcgg tattacattt ccatcaagaa aatggaaatc
     4621 gtattacagc tgtctttaaa cgtgtaagta agaaaaatgt ggcgccagat gatcagctat
     4681 atattttaga cgaagaaaat accataatgt cttgccaaca agctattgat actcaaagtc
     4741 aagatagcta taacttaggt ttgaatattt atgtggcaga tactaaatgg ttgattgctg
     4801 aattgaagaa agcccaaaaa tgtggcgctc ctattgatat tggtcaaaga tttgcaagtc
     4861 ttgcagcaaa ataccgtaca aatgcttatg aatataccgg ttatttgcat aatgtccatg
     4921 acattaagag ttattacgat tccaatattg aaatgcttga taagaaaaat cgtgactcac
     4981 ttttacacgg taatcaaaga attattacaa gaattagaaa tgaagtaggt acgtattaca
     5041 ctacttcttc taaagttaag aattctttat ttgcaactgg ctgtagaata aatggaacag
     5101 tagaaaatag tatcttatca agaagaatag atgttgctga agatagtaac gttaagaact
     5161 ctattgtaat ggcaagttgt actttagaag ccggtagtag tgttgaatat gctattttag
     5221 ataaaaatgt agtaataaaa aaagatgtag tagttaaggg aaccccagat aatccagtgg
     5281 ttattaagaa gggtgacgtt gtaactaaag atttaattgt tgaataagga gaaatcgtgc
     5341 gagttttatt tactggtgct gaatgtgcac cttttttcaa aactggggga ttgggcgatg
     5401 tattaggctc tttgcctaac caattagcta aagaaggtgt tgatgttggt gtcgtccttc
     5461 ctttgtatca agatttacca gaaaagtatc gtaaaaattt aaaatatcaa ggtaacttta
     5521 ttgtgccagt aggttggcgt aatcaatatt gtggaatttt tactttgaaa ttaaatggtg
     5581 tgaattactt ctttattgat aatgaatatt attttaagcg tccgggaatt tatggttatt
     5641 atgatgatgg cgaaagatat gcttatttcc aacaagcagt catcatgatg atggagcgtt
     5701 ttgattttat tccggatgtg ttgcattgca atgattatca cactgcattt attccatttt
     5761 tactccatga aaaatggggc tttgtcgatg catataaagg gattaaaaca attttaacta
     5821 ttcataactt agagtttcag gggaaatata atgcaaaaac tttaccggat ttctttggta
     5881 tgaattacga ctggtttgat agtggtattg tccgaatgga taatgatgtt aactggatga
     5941 aaaccggcat tttatatgca gaccgtgtaa ctacggttag tccatcttat gctcgtgaaa
     6001 ttcaaactgt tgaattcgga caaggcttag atgcgatttt aagaatgtgt tcacataagt
     6061 taactggtat tttaaatggg attgattttg aaaaatataa tcccaagact gatcccgtaa
     6121 ttaagaaaaa ttatgatgtt tatcatttac atcataaatc aaaggataaa atagcacttc
     6181 aaaaagaatt agatttgcca attaagccaa atattccttt aattggtatg gtttcacgtt
     6241 taactgcaca aaagggctgt caattattgc ttgacgagtt agataatatt ttgcaattta
     6301 atgttcagat tgtaatttta ggtaatggtg atccatatta tgaacatcgt ctaatggaaa
     6361 ttgctgagcg ttatccagat aaactaaagg ttattttagc gtttgatgtt aaattggctc
     6421 aaagaattta tgctggagca gatagtttct taatgccttc tgcgtttgaa ccgtgtggac
     6481 tttcacagct aattgcattg cgttatggta ctttaccaat tgttcatgaa attggtgggt
     6541 tagcagatac tgtttgggta tatgatgaga ctaccaatga aggtactggc tttggcttta
     6601 aggaatttag tggttatcaa atggtacaag caataaagaa aatgttagcg ttgtatcaac
     6661 aaaagaataa ttggcttaaa atacaaaaaa tagccatgaa atcggacttt tcatggaaaa
     6721 attctgctga taagtataag tggatgtatg gagagttaat tgggtaatcc aattggtgta
     6781 aaaatatgca attaacaaaa gatgaattta aaaagaaact aaaaaataag gtaaatattt
     6841 attttgaaga agaacttgat gaagcatcaa atagtgagtt atacactgca ttaagtggag
     6901 tggttagaga tggatatgca ccgcaatggc gtcgtacccg tatttccgaa gctgatcaag
     6961 gacaaaaaca ggtttattat ttttcaattg aatttttgcc aggaacttta cttaaaacta
     7021 atctgctaaa tttaggctgg ctggatactg ttagagctgc attgtcagat ttgggattag
     7081 atctagacaa gattgctgca gcagaaccag atatggcttt aggtaatggc ggtctgggac
     7141 gattggctgc agcttttatg gattctcttg cttcaactgg ctataccgga aatggaaatg
     7201 gtattcgtta taagtatggt ttattcaagc aaaaatttgt aaatggctat caaaaagaac
     7261 taccaaatga ttggcttaaa aaagacgatc attgggaagt tagaagagag agcaaatctg
     7321 ttttagtaag atttggcggt aaagtacgta tggttgatga taatgggtgg atgacacctc
     7381 aatatgaagg tggcgatgtt gtcagagctg taccgtatga tactgcaatt gtaggttata
     7441 gagatggtgt aaccaataca ttgagattgt gggatgcaga aattccacca gaagaagaat
     7501 tgtcatatcc tactatttca gatagacgtc gtatagaaga tttaacttca attttgtatc
     7561 cagatgattc taattatgaa ggtagattat taagattaaa gcaagaatat ttctttgttt
     7621 cagctggatt acaaagtatt ttagattatt atgttaaaaa acttggtaat aaagatttca
     7681 ctaaattacc agattacgtg gctgttcata tcaatgatac gcatccagca atggcgattg
     7741 cagaattgat gagattgcta gttgatgaac atcgtgtcga ttgggagact gcctgggata
     7801 tcactttaaa ggtgatgagt tataccaatc atacaattat gtcagaggca atggaaaaat
     7861 gggatacaaa tatgctgtct caattgttgc cgagaattat gcaaattatt actgaaatag
     7921 atcgtcgtta ttgtgcttat ttaaacggac aagtctctaa tgatgtaatt gaacgtactc
     7981 gaattataaa aaatggacag gtccaaatgg cgcatttagc aattattggc tcacactcaa
     8041 ttaatggggt agcagcattg cacacccaat tattggaaac caaagtgtta aaagattttt
     8101 ataatttata tcctgatcgc tttaataata aaactaatgg aatcactttg cgtagatggc
     8161 tacaaattgc taatccagaa ttatctgatt tacttgatca aactattggt aaagattggc
     8221 gtaaaaatag tgataagatg ctcaattttg aaaagtacta caacgacact ttagtattag
     8281 agcgtattaa tcaaattaaa ctagataata agaaaaaact agctgagttt atcaaagaac
     8341 aaatgggagt agaagttgat ccaaacgcta tctttgatgt gcaagtcaag cgactacatg
     8401 aatataaacg ccagacttta aagttattac atattctaaa gttatatcaa gatttaaaag
     8461 cagggattga tcatcctaag cgagtagtta tatttggggc taaagctgca ccaagctatg
     8521 tatttgctaa acaagtaatt aaagtgatca atgaaacagc taatatgatc aattcagatc
     8581 cagatattaa cggcaagtta aaggtaatct ttttagaaaa ttatgatgtt tctctagctg
     8641 aaaaaattat tcccgcagct gatgtaagcg agcaaatttc gaccactact aaagaagcta
     8701 gtggtacaag caacatgaaa ttgatggcca atggtgcttt aactgtggct acaatggatg
     8761 gtgcaaatat tgaaattgcg gatgcagtag gtgaggataa tatcattacg tttggcttaa
     8821 ataaggatca ggtttataaa tattatgcag aacattctta tcacccaaga gaaatgtacg
     8881 aatcagatcc agtaatgaag aaaacagttg atgcgttaac tgatggtaca attcctaatt
     8941 gtttttcgga aggacaagct ctagcaaata aatttttgag tgataatgag caattctttg
     9001 ttttagcaga ctttgctgat tatttaaaag ctcaagagaa agtagaacaa gaatggaaag
     9061 ataaacattc ttgggctcaa atgagtttgg taaatattgc tcattcagaa cgttttgatg
     9121 ttgataagac aattgaacga tacgcacaag atatttggca tttaaaaaaa ttaaaagttg
     9181 aaaaagttga tcactaatga aaataactta tgattcttgg caagagcagt ataaaaaccc
     9241 ttttggtgct gttaaagctg gtaatacagt taaatggtca atcaaaatag atcaagtaat
     9301 acaaggagct gtcttgtggt tgactaaaag tcgagaaaca ccagtggctt atccgatgaa
     9361 ctatgatgaa cagactaaaa tgtataccac acaggtaaaa attggtacat ctggtttata
     9421 caattactac tttgctttgc aacaaaataa tcaaatagtt tatatagatc aaggactttt
     9481 tggtaaagga cacgtaacta aaagtgatca tgatttaaga cagtttcaat taacttgtta
     9541 tgatattgca actccacgaa tcgattggta tcaaaagggc attgtttatc aaatttttcc
     9601 ggatcgcttt gctaatggaa atccatatga agaagttata ggtaaaaaaa ggaatagttt
     9661 tatctatgct actaaagaag atattccata ttatataaaa aatagtgaag gtgctatagt
     9721 gcggtgggac ttttttggcg gtaatttaac tggaattaga aaaaagatcc cttatttaaa
     9781 acaactaggt gttacagtcc tttatcttaa tccaattttt ttagctaaaa gtaatcatcg
     9841 ctatgataca acagatttta tgaaaatcga tccaatgttg ggtgatgaaa aagatttagc
     9901 tgacttaatt agggaattac atgagaataa tatgcatcta atattagatg gagtatttaa
     9961 tcatgtaggt tttgatagca tttattttca aggagcgatt actgacaaaa atagtaatta
    10021 tcgttcttgg ttcaactttc aagactatcc aaataaatat cagtcttggt ggggtgtaaa
    10081 gtctttaccg actgttaata aagataattc tgaatatcaa aatcttgttt atggcgatca
    10141 tggtgtatta gctaagtgga aagttgatgg ttggcgactt gatgtagctg atgagttacc
    10201 gatggatttc ttgcgtaata ttcgtaaccg tttgataaag gaaaattgtc caattttgat
    10261 tggggaagtt tgggaagatg cttcaaataa atttgtaaat ggtgaatatc gaacttatac
    10321 agctggtgat aatttaatgg gggttatgaa ttatccgata cgcaatttta ttatcagttt
    10381 gttatcagct caagatagta ctatagagat agaagcaatg aatgacctgg ctttactgat
    10441 tgaaaattat ccaactgact ttttgcataa ttgtttaaat aatatcggta cccatgatac
    10501 ggtaagaatt aaaaccgttt tgaataaaaa tgataattta gtcatgatgg cttttggctt
    10561 attatttatg atgcccggag ttccatgtat ttattatggc gatgaggctg gcttaattgg
    10621 taaagaagat ccagataatc gacgttactt tctatgggga catgaagata aaaagctaat
    10681 agattgtgtt agcagttgga ccaaaatacg caagcaaaat ccagtattgg taaatggcaa
    10741 aatcggtttt gtacatttgt cggctggggt taatagtata gttagatata atgatcaaga
    10801 gatgattatg tattgcgtta attgtactaa cgaagatgtg ataccgttgc gtgaaaagta
    10861 ttcattttat tggttgccga gcataattat tgataaaatc aaggatactt tggaccaaat
    10921 tcaacttaaa gcacaaacag attttattaa gaaaatatcg ctatagttat ttaaattagt
    10981 taaatttaag ttttataata atacgtggat aatagaaaaa tattgataat ttaaaaagac
    11041 attagaaatc cataaatata cgattttaat gaatagagga aaagatggaa gctaaggaaa
    11101 tttttaaaga atggcaaaat gctactgggt taccagatta tttaaaagat caattagata
    11161 ctttaggtaa agatgaaaaa tggattgaag atgcctttgg tcaggatatt aattttggta
    11221 ctgcaggaat gcgcggacgc cttgaaccag gtactaatcg aatcaatctg tttactgtgg
    11281 gtcgcgttac tgaaggttta gcgcgtttga ttgatgaaaa tggtgaggaa gctaaaaaac
    11341 gtggagtagt aattgctttt gactcaagat atcattcaag agaatttgca gaacacgctg
    11401 ctagagtact tggtgcacat ggtattcatg tttacttatt tgatgaccta agaccaactc
    11461 cagaattatc atatgcagtt cgtcacttaa atacttttgc aggaataaat atcactgctt
    11521 cgcataatgc taagcaatat aatggataca aggtttacgg tgaagatggt gcacaaatgg
    11581 ccccagaaaa tgccgatcgt ttatttgctt atgcacaaaa agttactgat atttttggtg
    11641 ttgaagttgc ttcagttaaa gaattacgcg caaataatac tttgcaatta attggtgaag
    11701 atgttgatga agcttattta aatgaattaa agcaagttac agttgataaa gaaatggtaa
    11761 aagctaatgc tgacaagctt aaaatcattt atactccact tcatggtaca ggtaaaatgc
    11821 tttatgatcg tgcatttaga caaggcggtt ttgacaatgt aattcctgtt ccaagtcaat
    11881 caattatcga tccagaattt ccaacaacta ttaagcctaa cccagaatat cgtgatgtat
    11941 ttgaaccagg ctttaagctg gctgataaag tagatgccaa tgtaattatt gcaactgatc
    12001 ctgatgcaga tagaatgggt gcagctgtga gaaaatccga tggtgatttc caagttctaa
    12061 ctggtaatca aattgctact ttaatggcat attatttgtt agctcacctt aaagagagtg
    12121 gcaagttaac tgatgattat gaaattgtta cttcagtagt ttcaagtgca ttgccattta
    12181 agattgccga agattttggt attaagacta aacatgtttt aactggtttt aagtatatcg
    12241 gtgaagaagt tgaccgtatg aataaagctg gagatgctaa gttcttaatg ggctttgaag
    12301 agagttatgg ttacttattt aagccttttg cacgtgataa agatgctatg caaggtgcat
    12361 taatgtttgc tgaagttgca agctactatg cttctcgtgg tatgaccgta tttgatggct
    12421 tacaagaaat ttggaagaaa tatggtgtcg cttacgaaat tactcgtgcc attgaaatgc
    12481 ctggtattgg tggtcagaag aagatggctg aattgatgag caagcttcgt aatgaacatc
    12541 ttactgaaat tggcggtagc aaagtagtta aaattcaaga ctttttaaaa caagaaacaa
    12601 ttgaaaatgg tgaaaagaca ccacttgaag gtttcccaga atcaaatgtt ttgaaatact
    12661 tcttagatga tgaaacttgg gttgctcttc gtccatcagg cactgaacca gttattaaag
    12721 cttatgttgg tgtaaataaa gaaaatattg aaaaagcaga aaaggctgca gaagattacc
    12781 aaactgcctt ggcaaattta cttaaataat caagcatatt gtgtgcgaaa acacgttcgt
    12841 ggtagtaaaa tataaatact aattagagaa gttggacaaa aagtccaact tttttcgtga
    12901 ggagctttgc tatgataaaa cgacaaaaag atcgtaagtt tgaattagta tcaaaattta
    12961 agccggcagg ggaccagcag caagctatca ataagttgac agcgggtttt gaaaagggat
    13021 ataaggaaca aattttagag ggtgccactg gtactgggaa gacttttacc atggctaaca
    13081 ttattgctaa gcttaacaag ccaactttgg tcattacaca taataaaaca ttggttggtc
    13141 agctttatgg cgaatttaag ggatttttcc ctaataatgc ggttgaatat tttgtctcat
    13201 actatgatta ctatcaacca gaagcgtatg ttccacaatc cgatacatac attgaaaaag
    13261 attctgcaat taatgatgaa attgatcaat tgcggcatgc tactacttca gctttaatgg
    13321 agagaaatga tgtaatagtt gttgcttcgg tttcatgtat ttatgggttg ggtgatccta
    13381 aagaatatgc tcgtagtgta ttaatgattc atgaaggaca agaatatgaa cgtaatacgt
    13441 tattacgtga tttagtaaac ttgcaatatg atcgaaatga tattgatttt caacgaggac
    13501 gttttcgggt ccgtggtgac gttgtagaaa ttttcccagc aggaaattct aaccatgcct
    13561 atcgtgttga atttttcggt gatgaaattg accgaattgt tgagatcgat tctttaactg
    13621 gggaagtaat tggtgagcgt gagagtatat cgctgtttcc ggctacgcac ttcatgacta
    13681 atgatgaaca attaagacga gctcttaagg cgatttctaa agaaatgaaa gtgcaagtta
    13741 aaaagttcga aggcgaaggt aaattgcttg aagccgaaag aattaaacaa agaactacct
    13801 atgatatgga aatgatggga gaagttggtt ataccaacgg aattgaaaat tactcaaggc
    13861 atatggaagg tagaaaagta ggagagccac catatacttt gcttgatttc ttcccagatg
    13921 attttttgat tttaattgat gaatctcatg caaccatgcc agaaattaga gcgatgtaca
    13981 atggagatcg taatcgtaaa aagaccttaa ttgattatgg ctttcgttta ccatcggcac
    14041 ttgataaccg tccactaaag ttggcagaat ttgaaaaaca tgttaaccaa attttatacg
    14101 tttctgctac tcctggagat tatgaacttg agaggacaga tcacaaggta gagcagatta
    14161 ttcgtccaac gggattattg gatcctaaga ttgaagtaag gccaatcgaa ggacaaattg
    14221 atgatttagt cggagaaata aataagcgga tagatcgtaa tgaacgtgtt tttgttacta
    14281 ctttaactaa aaagatggcc gaagatctga cagattattt aaaagatctg ggtattaaag
    14341 ttcgttattt acattcagat attaaaacgc ttgaaagaat gcagatctta cgtgatttac
    14401 gtttgggtaa atttgatgta ctgattggga ttaacttact tcgtgaaggt attgacgtgc
    14461 ctgaggtatc gctagtggcg attttagatg cagataaaga aggttttctt agagcatacc
    14521 ggccattagt tcaaacaatg gggcgtgcag cacgtaatgc aaatggagaa gtaattatgt
    14581 atgctgatac gattaccgat tcgatgaaag cagctatcga ggcaacccag agaagacgca
    14641 agttacagga agaatttaat aaagaacacg ggattgttcc taagacgatt attaaacctg
    14701 ttcatgatgt tatttcaatt actaagccat ctgaagactc taagaaagaa aaaacagata
    14761 gttttgcaga tttgaatttt gatgaattaa cagctaaaca aaagaagacg atgattaaga
    14821 atttgcaaga acagatgaaa gaggccgcta agaaacttga ctttgaagaa gcagcaaact
    14881 tgcgggatgc aataatggaa ttgcagtcat catctagaag accaaaaaca agaaaaggga
    14941 aggcattaaa tggtaaacga taa
//
