LOCUS       CP000303                7519 bp    DNA     linear   BCT 31-JAN-2014
DEFINITION  Bifidobacterium breve UCC2003, complete genome.
ACCESSION   CP000303
VERSION     CP000303.1
DBLINK      BioProject: PRJNA13487
            BioSample: SAMN02604112
KEYWORDS    .
SOURCE      Bifidobacterium breve UCC2003
  ORGANISM  Bifidobacterium breve UCC2003
            Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
            Bifidobacterium.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   O'Connell Motherway,M., Zomer,A., Leahy,S.C., Reunanen,J.,
            Bottacini,F., Claesson,M.J., O'Brien,F., Flynn,K., Casey,P.G.,
            Moreno Munoz,J.A., Kearney,B., Houston,A.M., O'Mahony,C.,
            Higgins,D.G., Shanahan,F., Palva,A., de Vos,W.M., Fitzgerald,G.F.,
            Ventura,M., O'Toole,P.W. and van Sinderen,D.
  TITLE     Functional genome analysis of Bifidobacterium breve UCC2003 reveals
            type IVb tight adherence (Tad) pili as an essential and conserved
            host-colonization factor
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2011) In press
   PUBMED   21690406
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   O'Connell Motherway,M., Zomer,A., Leahy,S., Reunanen,J.,
            Bottacini,F., Claesson,M., O'Brien,F., O'Flynn,K., Casey,P.,
            Moreno-Munoz,J., Higgins,D.G., Shanahan,F., de Vos,W.,
            Fitzgerald,G.F., Ventura,M., O'Toole,P. and van Sinderen,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2006) Department of Microbiology, Alimentary
            Pharmabiotic Centre, University College Cork, College Road, Cork,
            Munster, Ireland
COMMENT     Source DNA or strain is available from Douwe van Sinderen,
            d.vansinderen@ucc.ie.
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Bifidobacterium breve UCC2003"
                     /mol_type="genomic DNA"
                     /strain="UCC2003"
                     /db_xref="taxon:326426"
     gene            complement(1..867)
                     /locus_tag="Bbr_1865"
     CDS             complement(1..867)
                     /locus_tag="Bbr_1865"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00751|3.A.1.1.28"
                     /protein_id="ABE96527.1"
                     /translation="MTTATVTPSKSGKPAKFRRDHKINWWLTAAVAVLSLTILIPLYFT
                     IVTALKTPAEAGTFALPTSWQWHNFADASAKVNYPKAALNSAIITVAAVVLTLLTNTFV
                     AYAVARNMDKRFFRFLYYFFIAAMFVPFPVIMLPIAKQMGSLHLDNQVGLIILYTVLGL
                     GTNLFIATGFIRSIPVSLEEAARIDGASTWRIFWTIIFPLMSPINATIAILTALWAWND
                     FLLPLIILTDQSNQTIPLAQYVFSSQFATNYPMAFSSYLMAMAPILIVYIFAQKWVVGG
                     VMRGAVK"
     gene            complement(886..1833)
                     /locus_tag="Bbr_1866"
     CDS             complement(886..1833)
                     /locus_tag="Bbr_1866"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00750|3.A.1.1.28"
                     /protein_id="ABE96528.1"
                     /translation="MSHATATKTAAPAAKSAAKKPKKKVSAFSTRKVDPAYYWMAVPAA
                     IIFAFFLYLPFLDGVKYSFTNSQGYGDYKFIGLKNYIALFQDNRVGHAYLFTFFIAILI
                     TVLINVIALFLSVMLNSKIAFKNGFRAIFFIPYTLSVLVIGYVFKYIFMNPLPELGKAL
                     GIKWLSTSLLTNEQLSWIPIVFLAVWQGIAYSVLIYLAGLQTIDDEIYEAAAIDGVNPW
                     QKFWKITFPLIGPFFTINLVLSMKNALGTFDQVVALTEGGPNSSTETVTYLIWKGGLTG
                     GEYAYQTANAVLFFIVLAIIAFVQLRISRSQEQI"
     gene            complement(1855..3144)
                     /locus_tag="Bbr_1867"
     CDS             complement(1855..3144)
                     /locus_tag="Bbr_1867"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q00749|3.A.1.1.28"
                     /protein_id="ABE96529.1"
                     /translation="MLSDSPHSTPRLKRLAVRTAAVACAIAVGFGLAGCGSSTAGTVTL
                     DFFQFKSEAADQFKSMVADFEKQNPTIKVNINNSANAQTDLRTRFVKNRVPDVITFNGD
                     ISFGNFAASGVFYDFTDEPIVDTLNPGMVQIAKNLVQTTDSSKKRLYGLPFAGNASGYI
                     YNKALFRKVGLDPENPPTTWAEFTDMLNTFKDAGINPLQGTVADAWTTQAPLASLTGTL
                     VPESKYTELKQGKTTFQELWKTAVEKESELFTYSTADTGVTYQQGTQNFAQGKAAIIPL
                     GTYAIPQILLINPDIELGFAQMPATDDASEQILTAGDDVMLTIGANTKYPEESMKLVEF
                     LMQKDQLDAYADAQSAITPLKDTYFGNEALETVRPFFEENRLADFCDHYIPSSINIGGY
                     LQTMVTSGNTDRFLNQMQTEWDKVQARTFE"
     gene            3317..4525
                     /locus_tag="Bbr_1868"
     CDS             3317..4525
                     /locus_tag="Bbr_1868"
                     /codon_start=1
                     /transl_table=11
                     /product="Transcriptional repressor, ROK family"
                     /protein_id="ABE96530.1"
                     /translation="MSDANAIPQWFSGSEHTHRVAAAIAQYGPIARTTLAQMLGLSQGA
                     LSRITSDLIYAGVIEELPAVAGTTGKLPERFTPKESSDRRGRPQTALVLCSNARTFVGV
                     KVHGTSIVSAAVNAHGEVVSGRHEIPIGADQSAEYVTSSIATLVRECSDDVAATGLPAP
                     TAVGVAVGGHVIDDSIVTFAPFLHWEGTTDLGAMVYEATGLPCGVFNDIDSLLVDASWF
                     GPGVGVDTFAVVTIGVGVGYSLAVHGEPVSYPDKSFGLVGHVLIDPEGPRCPSGHIGCS
                     QCLTDDSIAEQYSAIIGRAVTFDDFAHDARAHVPQASQLVHRTCFRLGSLVATVANIAM
                     PGHVMIAGESAFLAKLGTDSLRDGIRFYRHSQTQPVKFTIMDHDWQLWAKAAASRVIVK
                     HIG"
     gene            complement(4638..6953)
                     /gene="aga"
                     /locus_tag="Bbr_1869"
     CDS             complement(4638..6953)
                     /gene="aga"
                     /locus_tag="Bbr_1869"
                     /EC_number="3.2.1.22"
                     /codon_start=1
                     /transl_table=11
                     /product="GH36"
                     /protein_id="ABE96531.1"
                     /translation="MAQNTATFTGTAADGTALTAIYLVQPDANVAIGLVAAGDDLPRII
                     HWGRPLSNPETLLAAYDALKPQRVSGALDDTAWPSILPTQAESWIGEQRVVLRRDGIEL
                     FPKFTVTGMKFDGVVAASLDAVSGDSYTDVTGRARTTGPANVPGVAITARDEEQGVELE
                     WHLELLPGGLARQKAIVTNLFGAEAGAEAPLEIGKIELGFPLPESASEILTTTGHHLRE
                     RSPQRQPLTIGRFEKPQLAGRPDFDASLLLTAGVPGFGFEHGEAYSVHVGWSGNSVLSA
                     ERLPYTQGVIGGGELLFGGEVTLDDAAGEGQSSYETPWLFGSFGDGLNEIAARFHSYVR
                     SLHPRLFSHGRPVILNTWEAVYFDHNFDTLKALADKAAASGVERFVVDDGWFGSRRDDT
                     SGLGDWQISQDVWPDGPKSLKALADYVHAKGMEFGLWFEPEMVNPDSDVARNHPDWILS
                     PTAGRLPLQGRTQQVLDLTNPEAFDYIYSCMDQLVGELGIDYIKWDHNKLVTEPGSRRS
                     GRPAVHAQTIAVYEIFRGLKTAHPGLEIESCSSGGGRVDLGILEYADRIWVSDCVDPVE
                     RADIQRYTSLLVPPAMMGEHVGASPAHSTRRETSQELRMAMAFFGHMGIEWNLLKEPDD
                     ALAKLAVWVAEFKKHRDWFAIDTCVHADSSDPAVRLDGMVMPNRNAAIYRFAQLTTSQT
                     YPAAPVHLPGLDPKRKYRISPLDPSLDLEGLTNGQSTLGWWNEEGVVLTGEALQRYGIR
                     PPSLHPQQAVLFKAVALS"
     gene            complement(7070..7519)
                     /gene="mesJ"
                     /locus_tag="Bbr_1870"
     CDS             complement(7070..7519)
                     /gene="mesJ"
                     /locus_tag="Bbr_1870"
                     /EC_number="3.5.4.-"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-specific adenosine deaminase"
                     /protein_id="ABE96532.1"
                     /translation="MGYDEMMHGALELAKRAAAAGDVPVGAVVVNADGQIIGRGYNTRE
                     ADGDPLAHAEIIAMREAARALGSWNLSDCTLAVTLEPCPMCAGACIQTHIGTIVFGAWD
                     SKLGACGSIWDIPRDPHIGHAPEVHGGVAEADCQTLLSTFFAHRR"
ORIGIN
        1 ctacttcaca gcgcccctca tcactccgcc cacgacccac ttttgggcga agatgtagac
       61 gatgaggatc ggggccatgg ccatcagata gctggagaat gccatcgggt agttggtggc
      121 aaactgcgag ctgaagacgt actgggcgag cgggatggtc tggttcgact ggtcggtcag
      181 aatgatcaac ggcaggagga agtcgttcca ggcccacagc gcggtcagaa tggcgatggt
      241 ggcgttaatc gggctcatca gcgggaagat gatggtccag aaaatgcgcc acgtggacgc
      301 gccatcgatg cgggccgcct cttccagcga aaccggaatc gagcggatga agccggtggc
      361 gatgaacagg ttcgtgccca ggccgagcac cgtgtacagg atgatcaggc cgacctggtt
      421 gtctaggtgc agggaaccca tctgcttcgc aatcggcagc atgatgaccg ggaacggcac
      481 gaacatggcg gcgatgaaga agtagtacag gaagcggaag aagcgcttgt ccatgttgcg
      541 ggccacggcg taggccacga aggtgttggt cagcagggtg agcaccacgg cggccaccgt
      601 gatgatcgcg gagttgagcg cggccttcgg gtagttgacc ttggcggagg cgtcggcgaa
      661 gttatgccac tgccacgagg tgggcagagc gaaggtgccg gcttcggccg gggtcttcag
      721 cgcggtgacg atggtgaagt agagcgggat cagaatggtc aggctcagca cggccacggc
      781 ggcggtcagc caccagttga tcttgtggtc cctgcggaac ttggcgggct tgccggactt
      841 ggacggggta actgttgcag tagtcatttc ttatcctccc ctaaatcaga tctgttcctg
      901 gctgcgggag atgcgcagct gcacgaaggc gatgatcgcc agcacgatga agaagagcac
      961 ggcgtttgcg gtctggtagg cgtactcgcc accggtcagg ccacccttcc aaatgaggta
     1021 ggtgacggtt tcggtggagg agttcggacc gccctcggtc aaggcgacga cctgatcgaa
     1081 ggtgccgagc gcgttcttca tggacagcac gaggttgatg gtgaagaacg gtccgatcag
     1141 cgggaaggtg atcttccaga acttctgcca gggattgacg ccgtcgatgg cggcggcctc
     1201 gtagatttca tcgtcaatgg tctgcagacc agcgaggtag atgagcaccg agtaggcgat
     1261 gccctgccag acggcgagga aaacaatcgg gatccagctg agctgctcat tggtcagcag
     1321 cgaagtggac agccacttga tgcccagcgc cttgccaagc tcgggcaacg ggttcatgaa
     1381 gatgtacttg aacacgtagc cgatgaccaa cacggagagg gtgtagggaa tgaagaagat
     1441 ggcacggaag ccgtttttga aggcgatctt cgagttcagc atcaccgata ggaacagggc
     1501 aatcacattg atcagcactg tgatgagaat ggcgatgaag aatgtgaaca ggtaggcgtg
     1561 gcccacacgg ttgtcttgaa agagagcgat gtagttcttc aggccgatga acttgtagtc
     1621 gccgtatccc tgcgaattgg tgaaggaata ctttacgccg tcaagaaatg gcaggtagag
     1681 gaagaaggcg aaaatgatag ccgccggaac cgccatccaa taataggcgg ggtccacttt
     1741 gcgggtggag aaggcggaaa ccttcttctt gggtttcttg gcagcggact tagcggccgg
     1801 agcagcggtc ttagttgcag ttgcgtgtga catgatgttc tcctctccta ccgatcactc
     1861 gaaggtcctg gcttggacct tgtcccattc ggtctgcatc tggttcagga agcggtcggt
     1921 gttgccggag gtcaccatgg tctgcaggta gccgccgata ttgatggacg aaggaatgta
     1981 gtgatcgcag aagtcggcca gtcggttttc ctcgaagaac gggcgcacgg tttccagcgc
     2041 ctcattgccg aagtaggtgt ccttcagcgg cgtgatggcg gactgggcgt cggcgtaggc
     2101 gtcgagctga tccttctgca tgaggaattc gacgagtttc attgattctt cggggtactt
     2161 ggtgtttgcg ccgatggtca gcatcacatc atcgcctgcg gtgaggatct gctcgctggc
     2221 gtcatcggtg gccggcatct gggcgaaacc aagttcaatg tccgggttga tgagcaggat
     2281 ctgtggaatg gcgtaggtac cgagcgggat gatcgcggcc ttgccttggg cgaagttctg
     2341 cgtgccctgc tggtaggtga cgccagtatc ggcagtggag taggtgaaga gttcggattc
     2401 cttctcgact gcggttttcc acagttcctg gaatgtggtc ttgccttgct tgagctcagt
     2461 gtacttcgac tcgggaacca gcgtaccggt cagcgaggcg agcggggcct gggtggtcca
     2521 ggcgtcggcc actgtgccct gcaacggatt gatgccggcg tccttgaagg tgttgagcat
     2581 gtcggtgaat tcggcccacg tggtcggcgg gttctccggg tcgagaccta ccttgcggaa
     2641 cagcgccttg ttgtagatgt aaccggaggc gttgccggca aagggcagac catacaggcg
     2701 cttcttggag gaatcggtgg tctgcaccag gtttttggcg atctgcacca tgccggggtt
     2761 gagggtgtcc acgatgggct cgtcggtgaa gtcgtagaaa acaccggatg cggcgaagtt
     2821 gccgaagctg atgtcgccgt tgaaggtgat tacgtccggc acgcggttct tgacgaagcg
     2881 ggtgcgtaga tcggtctggg cgttggcgga gttgttgatg ttgaccttga tggtcgggtt
     2941 ctgcttttcg aagtcggcga ccatggattt gaactggtct gcagcctcgg acttgaactg
     3001 gaagaaatcc agcgtaacgg tacctgcggt gctcgatccg cagcctgcca ggccgaagcc
     3061 gacggcgata gcgcacgcca cagcagcagt gcgcacggcc agacgcttca gtctaggcgt
     3121 tgaatgtggg gaatcggaca gcatattgtt gactccttta tctttttcct tccggcttca
     3181 gcgccggcgg ctcttccact ttcaaaagta acgattgcgc catttcattc ccagcgccag
     3241 cgagcccact tgcgcaagaa acaaaaagcg cggtattttg tgattaacgc aataaataat
     3301 gcaaaggggc ttaagtatgt ctgacgccaa tgcgattccg caatggtttt ccggatccga
     3361 gcatacgcat cgcgtggcgg cagccatcgc ccaatatggg ccgattgcgc gcaccacatt
     3421 ggcacagatg ctgggattga gccagggtgc gctctcccgc attaccagcg atttgattta
     3481 tgccggagtc atcgaagaac tgccggctgt ggccggaact acaggcaagc tgcctgaacg
     3541 ttttactccc aaggagagct cggatcgtcg cggccggccg cagaccgcat tggtattatg
     3601 ctcgaacgca cgcaccttcg taggcgtcaa agtgcacggc acatccattg tgtcggccgc
     3661 cgtgaacgcg catggcgagg tggtttccgg ccggcatgag ataccaattg gtgctgatca
     3721 gtcggcagaa tatgtgacat cgtcgatagc cacgctggtt cgtgaatgct ctgatgatgt
     3781 cgccgccacc ggcctgcccg ctcccactgc cgtgggcgta gcggtcggag gtcacgtgat
     3841 cgatgattcc atcgtcacgt tcgccccatt cctgcactgg gagggcacca cagatttagg
     3901 cgcaatggtg tacgaggcca ccggattgcc atgcggcgta ttcaacgata tcgattcgct
     3961 gctggtggat gcctcctggt tcgggccggg agtgggcgtg gatacgttcg ccgtagtgac
     4021 catcggcgtg ggcgtgggct attcgctggc tgtgcatggc gaaccggtca gttacccgga
     4081 caagagtttt ggcctagtgg ggcatgtgct catcgacccg gagggcccgc gctgccccag
     4141 tgggcatatc gggtgttcgc aatgcctgac tgacgattcg attgcggaac agtattcggc
     4201 gatcatcggc cgcgcagtga ccttcgatga ttttgcgcac gatgccagag cccatgtgcc
     4261 gcaagcctcc cagctagtgc accgcacttg cttccgattg ggatcgctgg tggccaccgt
     4321 ggcgaacatc gctatgccgg ggcatgtaat gatcgccggc gaatcggcat ttttggcgaa
     4381 gcttggtacc gattcgctgc gtgacggcat ccgcttctat cggcatagcc aaactcaacc
     4441 ggtgaagttc acgattatgg atcacgactg gcagctctgg gccaaagccg ctgccagccg
     4501 tgtaatcgtg aagcatattg ggtaggtcac tccgccacga ccagcaatta cggacttgcc
     4561 tatcggcaag gctcaatccc accttcactc agctatcgct tcgctcaggc catggctact
     4621 gacagcccac tgggctgtca gcttaacgcc acggccttga acagtactgc ctgctgaggg
     4681 tggagggacg ggggacggat gccgtagcgc tgcagggctt cgccggtcag gactacgcct
     4741 tcttcgttcc accaaccgag ggtgctttga ccattggtca gaccttcgag gtccaggctt
     4801 gggtcgagcg ggctaatgcg atacttgcgc ttggggtcaa ggccaggcag atgcacggga
     4861 gcggcaggat aggtctggct cgtggtcagc tgggcgaagc ggtagatggc agcattgcgg
     4921 ttcggcatca ccatgccatc gaggcgcacg gccggatcgc tggagtcggc gtgcacgcag
     4981 gtatcgatgg cgaaccaatc acgatgcttc ttgaactcgg ccacccacac tgccagcttg
     5041 gcaagtgcat cgtccggctc cttgagcagg ttccattcga tgcccatgtg cccgaagaat
     5101 gccatcgcca tgcgcaattc ctgactggtc tcacgacgag tggagtgcgc ggggctggca
     5161 cccacatgct cgcccatcat ggcaggcggc acaagcagtg atgtgtaacg ctggatgtcc
     5221 gcgcgttcga ccggatccac gcaatcggac acccagatac ggtcggcgta ttcgaggata
     5281 ccgagatcca cacggccgcc accggaagag cagctttcga tttcgagacc cgggtgagcg
     5341 gtcttgaggc ccctgaaaat ctcatagacg gcaatagttt gcgcatgcac ggccggacgg
     5401 ccggagcggc gagaaccggg ctcagtgacc agcttgttgt gatcccattt gatgtagtcg
     5461 atgccgagct cgccgacgag ctgatccatg cagctgtaaa tgtaatcgaa agcttccgga
     5521 ttggtcaggt cgagcacttg ctgagtgcgg ccctgcagcg gcagacggcc ggcagtgggc
     5581 gaaagaatcc aatcagggtg gttgcgggcc acgtcggaat cggggttgac catttccggc
     5641 tcgaaccaca ggccgaactc catgcccttg gcatgcacat aatcggcgag cgccttgagg
     5701 gacttcgggc catcgggcca tacgtcctga gaaatctgcc agtcgccaag gccggaggtg
     5761 tcgtcgcggc gagaaccgaa ccaaccgtca tccaccacga atcgttccac accagaagcg
     5821 gctgccttgt cggcgagcgc cttgagcgta tcgaagttgt ggtcgaagta cacggcttcc
     5881 caggtgttga ggattaccgg acggccgtgc gagaacaagc gcgggtgtaa agaacgcaca
     5941 taggagtgga agcgtgcggc aatctcgttc agaccatcgc cgaaggagcc gaacagccat
     6001 ggggtttcgt aagagctctg gccttcgccg gctgcatcat caagcgttac ttcgccgcca
     6061 aacagcagct cgccgccgcc aatcacaccc tgcgtgtacg gcagtcgctc ggcggacagc
     6121 acggagttgc cgctccagcc cacgtgcacc gagtaggctt cgccatgctc gaagccgaag
     6181 ccgggaacgc cggcggtaag cagcagggag gcgtcgaaat cagggcgacc cgcgagctgc
     6241 ggcttctcaa aacgtccgat ggtgagcggc tgacgctgcg ggctgcgttc gcgaagatgg
     6301 tggccggtgg tggtcaggat ttcggatgcg gattcgggta gcgggaaacc gagctcgatc
     6361 ttgccgattt ccagcggggc ctcggcaccg gcttctgctc cgaacaggtt ggtcacgatg
     6421 gccttctgac gggccaaacc accgggcaga agttcgagat gccattcgag ttccacgcct
     6481 tgctcttcgt cgcgcgcagt gattgccacg ccgggcacgt tggccgggcc agtggtacgg
     6541 gcacgtccag tcacatcggt gtagctgtcg ccggacacag catcgaggct agcagccacc
     6601 acgccgtcga atttcatacc ggtcacggtg aacttgggga agagttcgat gccgtcgcgg
     6661 cgcagcacca cacgctgctc gccaatccag ctttcggcct gtgtcggcag gatggacggc
     6721 caggcggtgt catcgagtgc accggaaacg cgctgcggct tgagtgcatc atacgcagcg
     6781 agcaaggtct caggattgct cagcgggcga ccccaatgaa tgatacgcgg caggtcatcg
     6841 ccggcagcga ccagaccgat tgccacgttg gcatcgggct gtacaagata gatggcggtg
     6901 agcgcggttc cgtcagcggc ggtaccggtg aaggtggcgg tgttctgggc catagtgtct
     6961 ccttcgacgt atacggaatg cattgaacta ctgattgtga tagtagaaga agtagaatat
     7021 gttgccttgg tgccgcgtgt tggatggatg aagtaagtat ttgagcgatt taccgccgat
     7081 gagcgaagaa cgtgctgagg agagtctggc aatcggcttc tgccacgccg ccatgcactt
     7141 caggggcgtg accaatatgg ggatcgcgcg ggatatccca gattgacccg caagcaccga
     7201 gcttggagtc ccacgcgcca aacacgatgg tgccgatatg ggtttggatg catgcgccgg
     7261 cgcacatcgg gcaaggctca agcgtaacag ccagtgtgca atcggagaga ttccacgagc
     7321 ctaatgcgcg ggcggcctca cgcatggcaa tgatttcggc atgcgccagt ggatcgccat
     7381 cggcctcgcg tgtgttgtat cctcgcccga taatctgtcc gtcggcgttc accaccactg
     7441 cgccaaccgg cacatcgccg gctgccgcgg cccttttggc gagctccagc gcgccgtgca
     7501 tcatctcgtc atatcccat
//
